AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0997310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGTGTAGGCTTGGCTTTC+TGG 0.089164 6_4:+76685576 Msa0997310:five_prime_UTR
TGTTTAGGCTGTTGAAGGTT+GGG 0.310010 6_4:+76685971 Msa0997310:intron
TGTTGGTTTGTTTGTGCTCA+TGG 0.333097 6_4:+76683962 Msa0997310:intron
ACTTATTTATATTTAGTAAT+GGG 0.349767 6_4:-76686207 None:intergenic
TAAAGTCAGGAGATGAATTA+TGG 0.353844 6_4:+76686015 Msa0997310:CDS
CCATACTTAGGTGGCTCATT+TGG 0.362701 6_4:+76685820 Msa0997310:CDS
ACTTGATGCAAGACATGCTT+TGG 0.365400 6_4:-76686344 None:intergenic
CTGGATTAGCATGGCACCTT+GGG 0.371240 6_4:+76685595 Msa0997310:exon
TGCAAGACATGCTTTGGAAT+TGG 0.380086 6_4:-76686338 None:intergenic
CCAAATGAGCCACCTAAGTA+TGG 0.382983 6_4:-76685820 None:intergenic
GTTTAGGCTGTTGAAGGTTG+GGG 0.389434 6_4:+76685972 Msa0997310:intron
TATCTTGTTTAGGCTGTTGA+AGG 0.389713 6_4:+76685966 Msa0997310:intron
TTGTTTAGGCTGTTGAAGGT+TGG 0.438805 6_4:+76685970 Msa0997310:intron
GACTTATTTATATTTAGTAA+TGG 0.441698 6_4:-76686208 None:intergenic
TACAATAGAGTGTTTGTTGT+TGG 0.465606 6_4:-76685330 None:intergenic
TTGGATGTTGCAGAAAGAAA+AGG 0.471032 6_4:-76686404 None:intergenic
GGATTCACGAAAACAGCAAA+TGG 0.474560 6_4:+76686053 Msa0997310:CDS
TCTGGATTAGCATGGCACCT+TGG 0.479623 6_4:+76685594 Msa0997310:exon
GTGTGTTTGTAGTGGAGACT+TGG 0.486501 6_4:-76686179 None:intergenic
GAAGCTGCATGAAAAGTAAG+AGG 0.486955 6_4:-76686257 None:intergenic
GACAAAGAATGGTGTAGGCT+TGG 0.487669 6_4:+76685568 Msa0997310:five_prime_UTR
TAAACTCAATGGACAAAGAA+TGG 0.488753 6_4:+76685557 Msa0997310:five_prime_UTR
GCAAAAGCAGGGTTAAAGTC+AGG 0.500832 6_4:+76686002 Msa0997310:CDS
ATAGAAATTATTTGTCTCGA+AGG 0.504218 6_4:+76686106 Msa0997310:exon
CTTGGCTTTCTGGATTAGCA+TGG 0.510281 6_4:+76685586 Msa0997310:exon
TTATATTTAGTAATGGGTGT+TGG 0.513099 6_4:-76686201 None:intergenic
GGGTGTTGGTGTGTTTGTAG+TGG 0.525806 6_4:-76686187 None:intergenic
TACTAAATATAAATAAGTCG+TGG 0.532381 6_4:+76686212 Msa0997310:three_prime_UTR
GTGAATCCAAGCTTATCTGC+TGG 0.535393 6_4:-76686038 None:intergenic
TTATGGCCAGCAGATAAGCT+TGG 0.539708 6_4:+76686032 Msa0997310:CDS
TGTTGTTGGTGTAGAAAATG+AGG 0.543974 6_4:-76685316 None:intergenic
TTTAGGCTGTTGAAGGTTGG+GGG 0.552652 6_4:+76685973 Msa0997310:intron
CTATGGCAGGTACCATACTT+AGG 0.555359 6_4:+76685808 Msa0997310:intron
ACTAAATATAAATAAGTCGT+GGG 0.563621 6_4:+76686213 Msa0997310:three_prime_UTR
CACTCACAGCAGGTTTCTCA+AGG 0.568836 6_4:-76685855 None:intergenic
CAATGGACAAAGAATGGTGT+AGG 0.571360 6_4:+76685563 Msa0997310:five_prime_UTR
TTGAAGACGACATAGCTGTA+AGG 0.582330 6_4:+76685282 Msa0997310:five_prime_UTR
GAGGAATTAATATAGCAAGT+AGG 0.597914 6_4:-76686238 None:intergenic
AAGTGATTTGGTGTGTGAGT+GGG 0.605770 6_4:-76683607 None:intergenic
GGTGTAGAAAATGAGGATGC+AGG 0.627573 6_4:-76685309 None:intergenic
TCACAAGAAAATGCATGACC+TGG 0.631525 6_4:-76686374 None:intergenic
GAAGTGATTTGGTGTGTGAG+TGG 0.631848 6_4:-76683608 None:intergenic
TTGGAGATAGAACAGCTGAA+AGG 0.635079 6_4:-76686319 None:intergenic
TCACAATCGCTGATTCGATG+AGG 0.682337 6_4:+76684068 Msa0997310:five_prime_UTR
TGGCAGGTACCATACTTAGG+TGG 0.694561 6_4:+76685811 Msa0997310:intron
GATGAAGCAGATCTGATGAG+CGG 0.792182 6_4:-76684008 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTATTTATATTTAGTAAT+GGG - chr6_4:76686210-76686229 None:intergenic 10.0%
!!! TAATTTCAAGTTTTTTTTGT+TGG + chr6_4:76684507-76684526 Msa0997310:intron 15.0%
!!! AATATTCAACAACATTTTTA+TGG - chr6_4:76684671-76684690 None:intergenic 15.0%
!!! GGAAACAAAATTATAAATTT+TGG + chr6_4:76684819-76684838 Msa0997310:intron 15.0%
!! TAATAAATTTATCATGTATG+AGG - chr6_4:76685894-76685913 None:intergenic 15.0%
!! GACTTATTTATATTTAGTAA+TGG - chr6_4:76686211-76686230 None:intergenic 15.0%
!!! GAATTATGATGTTTTAAGTT+TGG + chr6_4:76684144-76684163 Msa0997310:intron 20.0%
!!! TTTTTTTGTTGGTTTATTTG+TGG + chr6_4:76684518-76684537 Msa0997310:intron 20.0%
!!! AATTTTGGAATAGTAAACAT+AGG + chr6_4:76684834-76684853 Msa0997310:intron 20.0%
!!! ATTTTGGAATAGTAAACATA+GGG + chr6_4:76684835-76684854 Msa0997310:intron 20.0%
!!! TTCTTTTTTAGTAAACTCAA+TGG + chr6_4:76685546-76685565 Msa0997310:intron 20.0%
!! TAAGCATGTAAATTTGTATA+AGG - chr6_4:76685726-76685745 None:intergenic 20.0%
!! TACAAATTTACATGCTTATA+TGG + chr6_4:76685728-76685747 Msa0997310:intron 20.0%
!!! AAACTCAATAACTTTTATCA+TGG - chr6_4:76685782-76685801 None:intergenic 20.0%
!! TTATTGAGTTTAATTCACTA+TGG + chr6_4:76685791-76685810 Msa0997310:intron 20.0%
!!! ATTGATTCTAACTATAAAAG+TGG - chr6_4:76685918-76685937 None:intergenic 20.0%
!!! ACTTTTATAGTTAGAATCAA+TGG + chr6_4:76685917-76685936 Msa0997310:intron 20.0%
!! TACTAAATATAAATAAGTCG+TGG + chr6_4:76686212-76686231 Msa0997310:three_prime_UTR 20.0%
!! ACTAAATATAAATAAGTCGT+GGG + chr6_4:76686213-76686232 Msa0997310:three_prime_UTR 20.0%
! TGCTCTAATTTGATATTTCT+AGG + chr6_4:76683775-76683794 Msa0997310:intron 25.0%
!!! TGATGCAATTGACATTTTTT+AGG + chr6_4:76683815-76683834 Msa0997310:intron 25.0%
!!! GTTGTTTTTGTTTGTTTTGT+TGG + chr6_4:76683945-76683964 Msa0997310:intron 25.0%
!!! ATGTGATGTTTAAGTTTTGT+TGG + chr6_4:76684300-76684319 Msa0997310:intron 25.0%
!! ATGAGAATAAGATGATTTGT+AGG + chr6_4:76684339-76684358 Msa0997310:intron 25.0%
! ACTAATCAAAAGCTATACTA+AGG - chr6_4:76684964-76684983 None:intergenic 25.0%
!!! CTACTTTGAGGAAATATTTT+TGG - chr6_4:76684992-76685011 None:intergenic 25.0%
! AAGCACAAACATACATATAT+TGG - chr6_4:76685078-76685097 None:intergenic 25.0%
! TCTTTAATGCTCATATTGTT+AGG + chr6_4:76685097-76685116 Msa0997310:intron 25.0%
! CAGCTTGAAGTATAAATTTA+TGG - chr6_4:76685224-76685243 None:intergenic 25.0%
! TCTGTTGAAGATAAAATACA+AGG - chr6_4:76685370-76685389 None:intergenic 25.0%
! ACAATGAGAAAACTATTCTA+GGG - chr6_4:76685394-76685413 None:intergenic 25.0%
! TACAATGAGAAAACTATTCT+AGG - chr6_4:76685395-76685414 None:intergenic 25.0%
! CTAGTTATCTAACAAGTAAA+AGG - chr6_4:76685492-76685511 None:intergenic 25.0%
!! ACTTGTTAGATAACTAGATA+TGG + chr6_4:76685495-76685514 Msa0997310:intron 25.0%
!! TTATCAATTTTACTTACCCA+AGG - chr6_4:76685614-76685633 None:intergenic 25.0%
! AGATAGATAATTGCAATATC+AGG - chr6_4:76685951-76685970 None:intergenic 25.0%
!! TGCAATTATCTATCTTGTTT+AGG + chr6_4:76685956-76685975 Msa0997310:intron 25.0%
! ATAGAAATTATTTGTCTCGA+AGG + chr6_4:76686106-76686125 Msa0997310:exon 25.0%
!!! TTATATTTAGTAATGGGTGT+TGG - chr6_4:76686204-76686223 None:intergenic 25.0%
! GATTTTCAGATGAAGTGATT+TGG - chr6_4:76683622-76683641 None:intergenic 30.0%
AGAGCAAAATAAATACGCAA+AGG - chr6_4:76683761-76683780 None:intergenic 30.0%
GATTTGTAGGTTAATCTGAT+TGG + chr6_4:76684352-76684371 Msa0997310:intron 30.0%
TTTGTGGATGATTTATCTAC+CGG + chr6_4:76684534-76684553 Msa0997310:intron 30.0%
TAATCAAAATGCAGCAACAT+GGG - chr6_4:76684639-76684658 None:intergenic 30.0%
!!! TTGCTGCATTTTGATTAGTT+TGG + chr6_4:76684642-76684661 Msa0997310:intron 30.0%
AAAATTTGTGCACAGTAGTA+TGG + chr6_4:76684734-76684753 Msa0997310:intron 30.0%
TGTTGCTGAAAAATAAACTG+TGG + chr6_4:76684767-76684786 Msa0997310:intron 30.0%
GTTGCTGAAAAATAAACTGT+GGG + chr6_4:76684768-76684787 Msa0997310:intron 30.0%
AGCTTTCATTGATCATTAGA+TGG + chr6_4:76684798-76684817 Msa0997310:intron 30.0%
! AATCCAAAGTGTTTGTTAAG+TGG + chr6_4:76685051-76685070 Msa0997310:intron 30.0%
!! TACAATAGAGTGTTTGTTGT+TGG - chr6_4:76685333-76685352 None:intergenic 30.0%
ACAACAAACACTCTATTGTA+TGG + chr6_4:76685333-76685352 Msa0997310:intron 30.0%
TAAACTCAATGGACAAAGAA+TGG + chr6_4:76685557-76685576 Msa0997310:five_prime_UTR 30.0%
ATAGTTAGAATCAATGGTCT+TGG + chr6_4:76685923-76685942 Msa0997310:intron 30.0%
TAAAGTCAGGAGATGAATTA+TGG + chr6_4:76686015-76686034 Msa0997310:CDS 30.0%
GAGGAATTAATATAGCAAGT+AGG - chr6_4:76686241-76686260 None:intergenic 30.0%
TTTCCGTGTAATGAAAAAAC+TGG - chr6_4:76686296-76686315 None:intergenic 30.0%
! CACTTTTCTTCACGAATTTG+CGG + chr6_4:76683723-76683742 Msa0997310:intron 35.0%
CTAATCAAAATGCAGCAACA+TGG - chr6_4:76684640-76684659 None:intergenic 35.0%
TCCTCAAAGTAGATCTTCAT+TGG + chr6_4:76685000-76685019 Msa0997310:intron 35.0%
TCCAATGAAGATCTACTTTG+AGG - chr6_4:76685004-76685023 None:intergenic 35.0%
!! GGAGTAGCTTTTGATTAGTT+TGG + chr6_4:76685021-76685040 Msa0997310:intron 35.0%
TGTTGTTGGTGTAGAAAATG+AGG - chr6_4:76685319-76685338 None:intergenic 35.0%
TGAGTTTAATTCACTATGGC+AGG + chr6_4:76685795-76685814 Msa0997310:intron 35.0%
! TATCTTGTTTAGGCTGTTGA+AGG + chr6_4:76685966-76685985 Msa0997310:intron 35.0%
!!! CCTCCAGTTTTTTCATTACA+CGG + chr6_4:76686290-76686309 Msa0997310:three_prime_UTR 35.0%
!! AAGTGATTTGGTGTGTGAGT+GGG - chr6_4:76683610-76683629 None:intergenic 40.0%
!! TGTTGGTTTGTTTGTGCTCA+TGG + chr6_4:76683962-76683981 Msa0997310:intron 40.0%
AACTTGGATCGCAAGAGATA+CGG - chr6_4:76684588-76684607 None:intergenic 40.0%
CACGACAAAGTGAACAAACT+TGG - chr6_4:76684604-76684623 None:intergenic 40.0%
GGTCCACTTAACAAACACTT+TGG - chr6_4:76685057-76685076 None:intergenic 40.0%
TTGAAGACGACATAGCTGTA+AGG + chr6_4:76685282-76685301 Msa0997310:five_prime_UTR 40.0%
CAATGGACAAAGAATGGTGT+AGG + chr6_4:76685563-76685582 Msa0997310:five_prime_UTR 40.0%
ATTAATCTACCACTCACAGC+AGG - chr6_4:76685868-76685887 None:intergenic 40.0%
TTGTTTAGGCTGTTGAAGGT+TGG + chr6_4:76685970-76685989 Msa0997310:intron 40.0%
TGTTTAGGCTGTTGAAGGTT+GGG + chr6_4:76685971-76685990 Msa0997310:intron 40.0%
GGATTCACGAAAACAGCAAA+TGG + chr6_4:76686053-76686072 Msa0997310:CDS 40.0%
GAAGCTGCATGAAAAGTAAG+AGG - chr6_4:76686260-76686279 None:intergenic 40.0%
CCGTGTAATGAAAAAACTGG+AGG - chr6_4:76686293-76686312 None:intergenic 40.0%
TTGGAGATAGAACAGCTGAA+AGG - chr6_4:76686322-76686341 None:intergenic 40.0%
!! TGCAAGACATGCTTTGGAAT+TGG - chr6_4:76686341-76686360 None:intergenic 40.0%
ACTTGATGCAAGACATGCTT+TGG - chr6_4:76686347-76686366 None:intergenic 40.0%
! TTGCATCAAGTCTTTTCACC+AGG + chr6_4:76686356-76686375 Msa0997310:three_prime_UTR 40.0%
TCACAAGAAAATGCATGACC+TGG - chr6_4:76686377-76686396 None:intergenic 40.0%
!! GAAGTGATTTGGTGTGTGAG+TGG - chr6_4:76683611-76683630 None:intergenic 45.0%
GATGAAGCAGATCTGATGAG+CGG - chr6_4:76684011-76684030 None:intergenic 45.0%
!! TCACAATCGCTGATTCGATG+AGG + chr6_4:76684068-76684087 Msa0997310:five_prime_UTR 45.0%
GCAAATGCTCAGTGAAGTAC+CGG - chr6_4:76684556-76684575 None:intergenic 45.0%
CCACAATCGATACACGTGAA+CGG - chr6_4:76684709-76684728 None:intergenic 45.0%
GGTGTAGAAAATGAGGATGC+AGG - chr6_4:76685312-76685331 None:intergenic 45.0%
GACAAAGAATGGTGTAGGCT+TGG + chr6_4:76685568-76685587 Msa0997310:five_prime_UTR 45.0%
!! CTTGGCTTTCTGGATTAGCA+TGG + chr6_4:76685586-76685605 Msa0997310:exon 45.0%
CTATGGCAGGTACCATACTT+AGG + chr6_4:76685808-76685827 Msa0997310:intron 45.0%
CCAAATGAGCCACCTAAGTA+TGG - chr6_4:76685823-76685842 None:intergenic 45.0%
CCATACTTAGGTGGCTCATT+TGG + chr6_4:76685820-76685839 Msa0997310:CDS 45.0%
GTTTAGGCTGTTGAAGGTTG+GGG + chr6_4:76685972-76685991 Msa0997310:intron 45.0%
TTTAGGCTGTTGAAGGTTGG+GGG + chr6_4:76685973-76685992 Msa0997310:intron 45.0%
GCAAAAGCAGGGTTAAAGTC+AGG + chr6_4:76686002-76686021 Msa0997310:CDS 45.0%
TTATGGCCAGCAGATAAGCT+TGG + chr6_4:76686032-76686051 Msa0997310:CDS 45.0%
GTGAATCCAAGCTTATCTGC+TGG - chr6_4:76686041-76686060 None:intergenic 45.0%
! GTGTGTTTGTAGTGGAGACT+TGG - chr6_4:76686182-76686201 None:intergenic 45.0%
!!! TACTAATTTTATTATTTAAT+TGG + chr6_4:76685178-76685197 Msa0997310:intron 5.0%
!! CCGTTCACGTGTATCGATTG+TGG + chr6_4:76684706-76684725 Msa0997310:intron 50.0%
! ATGGTGTAGGCTTGGCTTTC+TGG + chr6_4:76685576-76685595 Msa0997310:five_prime_UTR 50.0%
!! TCTGGATTAGCATGGCACCT+TGG + chr6_4:76685594-76685613 Msa0997310:exon 50.0%
!! CTGGATTAGCATGGCACCTT+GGG + chr6_4:76685595-76685614 Msa0997310:exon 50.0%
TGGCAGGTACCATACTTAGG+TGG + chr6_4:76685811-76685830 Msa0997310:intron 50.0%
CACTCACAGCAGGTTTCTCA+AGG - chr6_4:76685858-76685877 None:intergenic 50.0%
CTTGAGAAACCTGCTGTGAG+TGG + chr6_4:76685856-76685875 Msa0997310:intron 50.0%
!! GGGTGTTGGTGTGTTTGTAG+TGG - chr6_4:76686190-76686209 None:intergenic 50.0%
TGGGGGAATGCAGCAAAAGC+AGG + chr6_4:76685990-76686009 Msa0997310:CDS 55.0%
GGGGGAATGCAGCAAAAGCA+GGG + chr6_4:76685991-76686010 Msa0997310:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 76683586 76686413 76683586 ID=Msa0997310;Name=Msa0997310
chr6_4 mRNA 76683586 76686413 76683586 ID=Msa0997310-mRNA-1;Parent=Msa0997310;Name=Msa0997310-mRNA-1;_AED=0.01;_eAED=0.01;_QI=380|1|1|1|0.4|0.33|6|304|65
chr6_4 exon 76683586 76683735 76683586 ID=Msa0997310-mRNA-1:exon:14439;Parent=Msa0997310-mRNA-1
chr6_4 exon 76683976 76684089 76683976 ID=Msa0997310-mRNA-1:exon:14440;Parent=Msa0997310-mRNA-1
chr6_4 exon 76685282 76685349 76685282 ID=Msa0997310-mRNA-1:exon:14441;Parent=Msa0997310-mRNA-1
chr6_4 exon 76685557 76685616 76685557 ID=Msa0997310-mRNA-1:exon:14442;Parent=Msa0997310-mRNA-1
chr6_4 exon 76685817 76685870 76685817 ID=Msa0997310-mRNA-1:exon:14443;Parent=Msa0997310-mRNA-1
chr6_4 exon 76685978 76686413 76685978 ID=Msa0997310-mRNA-1:exon:14444;Parent=Msa0997310-mRNA-1
chr6_4 five_prime_UTR 76683586 76683735 76683586 ID=Msa0997310-mRNA-1:five_prime_utr;Parent=Msa0997310-mRNA-1
chr6_4 five_prime_UTR 76683976 76684089 76683976 ID=Msa0997310-mRNA-1:five_prime_utr;Parent=Msa0997310-mRNA-1
chr6_4 five_prime_UTR 76685282 76685349 76685282 ID=Msa0997310-mRNA-1:five_prime_utr;Parent=Msa0997310-mRNA-1
chr6_4 five_prime_UTR 76685557 76685604 76685557 ID=Msa0997310-mRNA-1:five_prime_utr;Parent=Msa0997310-mRNA-1
chr6_4 CDS 76685605 76685616 76685605 ID=Msa0997310-mRNA-1:cds;Parent=Msa0997310-mRNA-1
chr6_4 CDS 76685817 76685870 76685817 ID=Msa0997310-mRNA-1:cds;Parent=Msa0997310-mRNA-1
chr6_4 CDS 76685978 76686109 76685978 ID=Msa0997310-mRNA-1:cds;Parent=Msa0997310-mRNA-1
chr6_4 three_prime_UTR 76686110 76686413 76686110 ID=Msa0997310-mRNA-1:three_prime_utr;Parent=Msa0997310-mRNA-1
Gene Sequence

>Msa0997310

ATGGCACCTTGGGTACCATACTTAGGTGGCTCATTTGGCATTATCTATATCCTTGAGAAACCTGCTGCTGTTGAAGGTTGGGGGAATGCAGCAAAAGCAGGGTTAAAGTCAGGAGATGAATTATGGCCAGCAGATAAGCTTGGATTCACGAAAACAGCAAATGGTTTTGTCTCTAAAGTTTCTATAGAAGAGAAATAG

Protein sequence

>Msa0997310

MAPWVPYLGGSFGIIYILEKPAAVEGWGNAAKAGLKSGDELWPADKLGFTKTANGFVSKVSIEEK*