AlfalfaGEDB | Msa0396520

M. sativa cultivar zhongmu-4 / Msa0396520

KEGG: K04506|K08742
GO: GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080134 GO:0080148 GO:0140096 GO:1901564 GO:2000070
PFAM: PF03145.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0396520	XP_003598630.1	100.000	304	0	0	1	304	1	304	0.0	640

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0396520	sp\|Q10L91\|DIS1_ORYSJ	83.113	302	49	2	3	304	2	301	0.0	530

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0396520	A8W462	100.000	304	0	0	1	304	1	304	0.0	640

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0209140	Msa0396520	0.811209	7.848314e-51	-8.615850e-47
Msa0288620	Msa0396520	0.801566	8.475031e-49	-8.615850e-47
Msa0349720	Msa0396520	0.947728	3.819709e-106	-8.615850e-47
Msa0396520	Msa1207330	0.827121	1.863681e-54	-8.615850e-47
Msa0396520	Msa1331300	0.832441	9.438401e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0396520	MtrunA17_Chr3g0083571	100.000	304	0	0	1	304	1	304	0.0	640
Msa0396520	MtrunA17_Chr8g0352701	84.539	304	47	0	1	304	1	304	0.0	556
Msa0396520	MtrunA17_Chr3g0126851	79.630	270	54	1	36	304	42	311	4.12e-169	471
Msa0396520	MtrunA17_Chr1g0147041	83.333	258	43	0	47	304	62	319	2.12e-168	470
Msa0396520	MtrunA17_Chr8g0346801	77.256	277	62	1	29	304	19	295	7.76e-165	460
Msa0396520	MtrunA17_Chr7g0275701	79.008	262	55	0	43	304	51	312	2.02e-162	454
Msa0396520	MtrunA17_Chr7g0258941	73.704	270	67	1	38	303	40	309	3.56e-153	431
Msa0396520	MtrunA17_Chr1g0175721	74.525	263	66	1	43	304	47	309	2.61e-152	428
Msa0396520	MtrunA17_Chr7g0223111	26.147	218	149	5	45	262	31	236	7.14e-22	94.0
Msa0396520	MtrunA17_Chr5g0433631	26.778	239	159	5	23	261	9	231	1.84e-21	92.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0396520	AT3G61790.1	82.239	259	46	0	46	304	53	311	1.44e-166	466
Msa0396520	AT4G27880.1	79.775	267	53	1	39	304	46	312	7.30e-165	461
Msa0396520	AT3G58040.1	79.070	258	54	0	47	304	51	308	4.05e-159	446
Msa0396520	AT3G58040.3	79.070	258	54	0	47	304	51	308	4.05e-159	446
Msa0396520	AT2G41980.1	78.295	258	56	0	47	304	48	305	2.34e-158	444
Msa0396520	AT5G53360.1	85.784	204	29	0	101	304	15	218	9.12e-136	384
Msa0396520	AT5G53360.3	85.784	204	29	0	101	304	15	218	9.12e-136	384
Msa0396520	AT3G58040.2	73.964	169	38	1	47	209	51	219	4.40e-88	263
Msa0396520	AT3G13672.1	58.333	168	67	1	140	304	45	212	1.84e-73	225
Msa0396520	AT3G13672.2	50.769	195	66	2	140	304	45	239	9.01e-68	212

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Find 88 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAACCTGGTGGATTGG+AGG	0.544752	3_2:-15051281	None:intergenic
AAACAACCTGGTGGATTGGA+GGG	0.552550	3_2:-15051280	None:intergenic
AAATGCCACGTGGATGTTAA+CGG	0.491591	3_2:+15052396	Msa0396520:CDS
AACTGTCACGAACCTTGCGG+TGG	0.618648	3_2:-15053098	None:intergenic
AAGTAGAAATGATGAGAAAT+AGG	0.232134	3_2:-15049634	None:intergenic
AATAATGTTTAGATGAAGAA+AGG	0.496248	3_2:-15053235	None:intergenic
AATGGTCACACTTTATGCTC+TGG	0.475297	3_2:+15052037	Msa0396520:CDS
ACATAGCATTCAAGCACACA+GGG	0.641099	3_2:-15051256	None:intergenic
ACCTACAGTCTTGAGGTAGG+TGG	0.614102	3_2:+15053024	Msa0396520:CDS
ACGAAGGGGCTGCTGATCGT+TGG	0.529410	3_2:+15051056	Msa0396520:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGATCAAAACCATCATTTC+AGG	+	chr3_2:15049678-15049697	Msa0396520:five_prime_UTR	30.0%
CAATGAAAACATTCATGAGA+GGG	-	chr3_2:15049939-15049958	None:intergenic	30.0%
TCAATGAAAACATTCATGAG+AGG	-	chr3_2:15049940-15049959	None:intergenic	30.0%
AAAATCTGCATACCATTTGA+GGG	-	chr3_2:15050109-15050128	None:intergenic	30.0%
AAAAATCTGCATACCATTTG+AGG	-	chr3_2:15050110-15050129	None:intergenic	30.0%
AACTTCAAGTATTCAAATGG+TGG	-	chr3_2:15050654-15050673	None:intergenic	30.0%
TGCCAAAAACAATTAGCAAT+AGG	-	chr3_2:15050936-15050955	None:intergenic	30.0%
AGACTGTTGTTAAACCTAAT+GGG	+	chr3_2:15051198-15051217	Msa0396520:CDS	30.0%
TCTCTAATTAAGAAAGCAAG+GGG	-	chr3_2:15051627-15051646	None:intergenic	30.0%
AGTAAGTATAGTAAGTGTCA+TGG	-	chr3_2:15051738-15051757	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	15049468	15053450	15049468	ID=Msa0396520;Name=Msa0396520
chr3_2	mRNA	15049468	15053450	15049468	ID=Msa0396520-mRNA-1;Parent=Msa0396520;Name=Msa0396520-mRNA-1;_AED=0.06;_eAED=0.06;_QI=259\|1\|1\|1\|0.5\|0.4\|5\|206\|304
chr3_2	exon	15049468	15049699	15049468	ID=Msa0396520-mRNA-1:exon:3339;Parent=Msa0396520-mRNA-1
chr3_2	exon	15051062	15051295	15051062	ID=Msa0396520-mRNA-1:exon:3340;Parent=Msa0396520-mRNA-1
chr3_2	exon	15052031	15052417	15052031	ID=Msa0396520-mRNA-1:exon:3341;Parent=Msa0396520-mRNA-1
chr3_2	exon	15052904	15053260	15052904	ID=Msa0396520-mRNA-1:exon:3342;Parent=Msa0396520-mRNA-1
chr3_2	exon	15053281	15053450	15053281	ID=Msa0396520-mRNA-1:exon:3343;Parent=Msa0396520-mRNA-1
chr3_2	five_prime_UTR	15049468	15049699	15049468	ID=Msa0396520-mRNA-1:five_prime_utr;Parent=Msa0396520-mRNA-1
chr3_2	five_prime_UTR	15051062	15051088	15051062	ID=Msa0396520-mRNA-1:five_prime_utr;Parent=Msa0396520-mRNA-1
chr3_2	CDS	15051089	15051295	15051089	ID=Msa0396520-mRNA-1:cds;Parent=Msa0396520-mRNA-1
chr3_2	CDS	15052031	15052417	15052031	ID=Msa0396520-mRNA-1:cds;Parent=Msa0396520-mRNA-1
chr3_2	CDS	15052904	15053224	15052904	ID=Msa0396520-mRNA-1:cds;Parent=Msa0396520-mRNA-1
chr3_2	three_prime_UTR	15053225	15053260	15053225	ID=Msa0396520-mRNA-1:three_prime_utr;Parent=Msa0396520-mRNA-1
chr3_2	three_prime_UTR	15053281	15053450	15053281	ID=Msa0396520-mRNA-1:three_prime_utr;Parent=Msa0396520-mRNA-1

Gene Sequence

>Msa0396520

ATGACATCTAGTAATCCTTTCTTTGATGATATACGGAGCAGACCTGGGGTTGATCCTCCTCAAACTGAAGAGTCAACAGAAATTCCTGAACTCGTAAATGATCCTATACAGACTGTTGTTAAACCTAATGGGACTGTTTCAAGCAGTGTTCGTGAGCTTTTAGAGTGCCCTGTGTGCTTGAATGCTATGTACCCTCCAATCCACCAGTGCTCAAATGGTCACACTTTATGCTCTGGTTGCAAACCAAGAGTCCATGACCGGTGCCCTACTTGTAGGCATGAGCTCGGCAATATTAGATGTCTGGCATTGGAGAAGGTGGCTGCATCTCTAGAACTTCCTTGTAAATATCAAGGTTTTGGGTGCATAGGCATATACCCATATTACAGCAAGCTAAAACACGAGTCTCAATGTGTTTTTAGACCCTATAACTGTCCATACGCTGGCTCAGAATGTGCTGTCGTCGGTGATGTACAATTCTTGGTGGATCACCTGAAAGATGATCACAAAGTTGACATGCATAGTGGCTGCACTTTTAATCATCGCTATGTCAAATCAAATCCACAGGAAGTTGAAAATGCCACGTGGATGTTAACGGTTTTTAGTTGCTTCGGTCAGTATTTTTGCCTGCATTTTGAAGCTTTTCAACTGGGAATGGCTCCTGTCTACATAGCATTTTTGCGGTTTATGGGCGATGATGATCAAGCAAAGAACTATACCTACAGTCTTGAGGTAGGTGGAAATGGGAGGAAGATGACATGGCAGGGAGTGCCTAGAAGCATTAGGGAAAGCCACCGCAAGGTTCGTGACAGTTTTGACGGTCTCATCATTCAGAGGAACATGGCTCTCTTCTTTTCCGGGGGTGATAGGAAGGAATTGAAACTACGAGTTACCGGAAGGATTTGGAAAGAGCAGTGA

Protein sequence

>Msa0396520

MTSSNPFFDDIRSRPGVDPPQTEESTEIPELVNDPIQTVVKPNGTVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHDRCPTCRHELGNIRCLALEKVAASLELPCKYQGFGCIGIYPYYSKLKHESQCVFRPYNCPYAGSECAVVGDVQFLVDHLKDDHKVDMHSGCTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDDQAKNYTYSLEVGGNGRKMTWQGVPRSIRESHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ*