Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399140 | RHN66131.1 | 86.154 | 65 | 9 | 0 | 1 | 65 | 1 | 65 | 3.20e-31 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399140 | A0A396IMW8 | 86.154 | 65 | 9 | 0 | 1 | 65 | 1 | 65 | 1.53e-31 | 113 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0399140 | Msa0627410 | 0.808516 | 2.981251e-50 | -8.615850e-47 |
| Msa0353190 | Msa0399140 | 0.801685 | 8.012023e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399140 | MtrunA17_Chr3g0087451 | 86.154 | 65 | 9 | 0 | 1 | 65 | 1 | 65 | 2.94e-35 | 113 |
| Msa0399140 | MtrunA17_Chr3g0087441 | 93.846 | 65 | 4 | 0 | 1 | 65 | 1 | 65 | 8.08e-32 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 7 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATTGTTGACAGGGAAATA+AGG | 0.339005 | 3_2:+21485190 | None:intergenic |
| TCAGTGTTCTTCAATTGCTT+TGG | 0.344671 | 3_2:-21485334 | Msa0399140:CDS |
| GGGAAATAAGGAACTACAAT+TGG | 0.362410 | 3_2:+21485202 | None:intergenic |
| GAGGAATAGTTATTGTTGAC+AGG | 0.418186 | 3_2:+21485181 | None:intergenic |
| TTCAGAGAAACCAAAGAGCA+AGG | 0.587633 | 3_2:-21485242 | Msa0399140:CDS |
| AGGAATAGTTATTGTTGACA+GGG | 0.594177 | 3_2:+21485182 | None:intergenic |
| TGCTAGTGACTACAAGAGTG+AGG | 0.660681 | 3_2:+21485162 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGATTTTTCCTTGCTCTT+TGG | + | chr3_2:21485288-21485307 | None:intergenic | 30.0% |
| AAAGAGCAAGGAAAAATCAA+TGG | - | chr3_2:21485287-21485306 | Msa0399140:CDS | 30.0% | |
| AAGAGCAAGGAAAAATCAAT+GGG | - | chr3_2:21485288-21485307 | Msa0399140:CDS | 30.0% | |
| TTATTGTTGACAGGGAAATA+AGG | + | chr3_2:21485330-21485349 | None:intergenic | 30.0% | |
| AGGAATAGTTATTGTTGACA+GGG | + | chr3_2:21485338-21485357 | None:intergenic | 30.0% | |
| TCAGTGTTCTTCAATTGCTT+TGG | - | chr3_2:21485183-21485202 | Msa0399140:CDS | 35.0% | |
| GGGAAATAAGGAACTACAAT+TGG | + | chr3_2:21485318-21485337 | None:intergenic | 35.0% | |
| GAGGAATAGTTATTGTTGAC+AGG | + | chr3_2:21485339-21485358 | None:intergenic | 35.0% | |
| TTCAGAGAAACCAAAGAGCA+AGG | - | chr3_2:21485275-21485294 | Msa0399140:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 21485171 | 21485368 | 21485171 | ID=Msa0399140;Name=Msa0399140 |
| chr3_2 | mRNA | 21485171 | 21485368 | 21485171 | ID=Msa0399140-mRNA-1;Parent=Msa0399140;Name=Msa0399140-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|65 |
| chr3_2 | exon | 21485171 | 21485368 | 21485171 | ID=Msa0399140-mRNA-1:exon:4687;Parent=Msa0399140-mRNA-1 |
| chr3_2 | CDS | 21485171 | 21485368 | 21485171 | ID=Msa0399140-mRNA-1:cds;Parent=Msa0399140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
