AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0408370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0408370 MtrunA17_Chr3g0099031 88.674 362 15 2 4 339 2 363 0.0 651
Msa0408370 MtrunA17_Chr3g0108941 67.493 363 91 3 4 339 2 364 7.08e-180 508
Msa0408370 MtrunA17_Chr3g0109001 66.942 363 93 3 4 339 2 364 3.27e-178 504
Msa0408370 MtrunA17_Chr3g0109021 67.403 362 92 2 4 339 2 363 5.46e-178 503
Msa0408370 MtrunA17_Chr3g0105691 66.575 362 95 2 4 339 35 396 3.05e-176 500
Msa0408370 MtrunA17_Chr3g0108991 66.575 362 95 2 4 339 2 363 5.48e-175 495
Msa0408370 MtrunA17_Chr3g0108951 66.575 362 95 2 4 339 2 363 5.48e-175 495
Msa0408370 MtrunA17_Chr3g0109031 66.851 362 94 2 4 339 2 363 3.78e-174 493
Msa0408370 MtrunA17_Chr3g0109011 68.249 337 81 2 29 339 2 338 4.00e-169 479
Msa0408370 MtrunA17_Chr3g0108981 68.343 338 80 3 29 339 2 339 8.34e-167 474
Msa0408370 MtrunA17_Chr3g0108961 68.343 338 80 3 29 339 2 339 8.34e-167 474
Msa0408370 MtrunA17_Chr3g0108971 67.359 337 84 2 29 339 2 338 5.05e-166 472
Msa0408370 MtrunA17_Chr6g0477111 65.579 337 90 2 29 339 2 338 5.40e-162 461
Msa0408370 MtrunA17_Chr2g0280581 60.606 363 113 3 7 339 5 367 2.03e-156 448
Msa0408370 MtrunA17_Chr6g0477121 69.751 281 83 1 61 339 1 281 2.12e-145 417
Msa0408370 MtrunA17_Chr4g0048911 44.345 336 160 4 29 339 76 409 5.22e-91 283
Msa0408370 MtrunA17_Chr4g0048921 42.647 340 169 7 24 339 80 417 1.38e-78 250
Msa0408370 MtrunA17_Chr2g0323131 33.714 350 199 7 15 339 72 413 4.94e-60 202
Msa0408370 MtrunA17_Chr2g0323151 33.435 329 192 6 33 339 6 329 1.51e-55 188
Msa0408370 MtrunA17_Chr2g0310331 30.726 358 222 6 6 339 79 434 4.74e-49 173
Msa0408370 MtrunA17_Chr8g0357411 28.969 359 228 8 6 339 81 437 1.40e-42 155
Msa0408370 MtrunA17_Chr4g0023911 30.685 365 219 9 4 339 69 428 5.33e-42 153
Msa0408370 MtrunA17_Chr4g0024691 29.310 348 218 6 18 339 63 408 2.73e-41 151
Msa0408370 MtrunA17_Chr2g0319041 27.701 361 228 6 10 338 45 404 7.86e-39 144
Msa0408370 MtrunA17_Chr4g0042111 28.846 364 221 8 9 339 51 409 2.57e-38 143
Msa0408370 MtrunA17_Chr2g0310401 31.970 269 180 3 74 339 12 280 4.48e-38 140
Msa0408370 MtrunA17_Chr6g0465851 28.448 348 221 5 18 339 20 365 5.39e-38 142
Msa0408370 MtrunA17_Chr6g0465911 28.448 348 221 5 18 339 55 400 7.34e-38 142
Msa0408370 MtrunA17_Chr6g0466051 28.161 348 222 5 18 339 55 400 7.79e-38 142
Msa0408370 MtrunA17_Chr7g0236961 28.035 346 219 6 21 339 3 345 1.12e-37 140
Msa0408370 MtrunA17_Chr7g0236991 28.080 349 221 6 18 339 55 400 1.45e-37 141
Msa0408370 MtrunA17_Chr6g0451711 27.536 345 222 5 21 339 62 404 1.72e-37 140
Msa0408370 MtrunA17_Chr7g0237011 26.857 350 218 4 21 339 3 345 4.77e-37 139
Msa0408370 MtrunA17_Chr7g0237001 26.912 353 220 4 18 339 55 400 6.12e-37 139
Msa0408370 MtrunA17_Chr7g0237041 26.912 353 220 4 18 339 55 400 6.12e-37 139
Msa0408370 MtrunA17_Chr7g0237081 26.912 353 220 4 18 339 55 400 6.12e-37 139
Msa0408370 MtrunA17_Chr7g0237051 26.912 353 220 4 18 339 55 400 6.12e-37 139
Msa0408370 MtrunA17_Chr6g0465441 27.507 349 223 6 18 339 55 400 8.02e-37 139
Msa0408370 MtrunA17_Chr3g0105071 26.781 351 218 7 21 339 61 404 3.40e-36 137
Msa0408370 MtrunA17_Chr5g0431651 27.624 362 220 9 8 339 50 399 2.84e-35 134
Msa0408370 MtrunA17_Chr5g0431571 29.545 352 207 10 21 339 53 396 4.37e-35 134
Msa0408370 MtrunA17_Chr5g0425371 27.557 352 219 7 18 339 55 400 1.73e-34 132
Msa0408370 MtrunA17_Chr3g0082691 27.119 354 219 10 18 339 54 400 6.25e-34 130
Msa0408370 MtrunA17_Chr7g0236971 28.916 249 174 2 93 339 1 248 1.89e-32 124
Msa0408370 MtrunA17_Chr4g0008621 25.138 362 236 7 9 338 52 410 1.22e-31 123
Msa0408370 MtrunA17_Chr2g0319051 25.905 359 237 6 9 338 3 361 3.60e-31 122
Msa0408370 MtrunA17_Chr6g0466111 28.859 298 184 5 18 289 20 315 1.82e-30 118
Msa0408370 MtrunA17_Chr6g0465981 28.859 298 184 5 18 289 55 350 3.11e-30 118
Msa0408370 MtrunA17_Chr6g0465871 28.859 298 184 5 18 289 55 350 3.11e-30 118
Msa0408370 MtrunA17_Chr6g0466081 28.859 298 184 5 18 289 55 350 3.11e-30 118
Msa0408370 MtrunA17_Chr6g0465931 28.523 298 185 5 18 289 20 315 3.59e-29 114
Msa0408370 MtrunA17_Chr7g0236951 26.415 318 204 6 21 311 3 317 3.89e-28 111
Msa0408370 MtrunA17_Chr7g0237031 25.155 322 203 4 21 311 3 317 1.48e-27 110
Msa0408370 MtrunA17_Chr5g0445511 29.245 212 141 4 89 297 2 207 3.80e-27 107
Msa0408370 MtrunA17_Chr8g0336181 25.141 354 219 9 22 338 59 403 4.17e-27 111
Msa0408370 MtrunA17_Chr7g0217361 34.211 152 99 1 189 339 6 157 6.07e-24 99.8
Msa0408370 MtrunA17_Chr8g0357231 38.318 107 65 1 234 339 1 107 1.96e-20 89.4
Msa0408370 MtrunA17_Chr7g0237061 38.679 106 65 0 234 339 2 107 5.85e-20 87.4
Msa0408370 MtrunA17_Chr2g0319081 26.712 146 106 1 194 338 140 285 5.54e-19 87.4
Msa0408370 MtrunA17_Chr6g0466031 33.000 100 67 0 240 339 8 107 1.86e-15 75.1
Msa0408370 MtrunA17_Chr2g0310121 22.992 361 228 12 26 339 271 628 2.30e-12 68.2
Msa0408370 MtrunA17_Chr2g0281651 28.655 171 116 4 171 339 45 211 3.94e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0408370 AT1G61680.2 36.705 346 200 8 12 339 74 418 1.50e-65 216
Msa0408370 AT1G61680.1 36.544 353 198 8 12 339 74 425 6.52e-65 215
Msa0408370 AT4G16730.1 30.028 363 223 7 8 339 21 383 8.48e-44 158
Msa0408370 AT4G16740.1 30.137 365 215 6 9 339 56 414 1.63e-42 155
Msa0408370 AT4G16730.2 29.970 337 206 6 33 339 1 337 3.57e-41 150
Msa0408370 AT4G16740.2 29.155 343 202 7 9 317 56 391 2.39e-34 130
Msa0408370 AT2G24210.1 27.424 361 232 7 8 339 74 433 7.11e-34 131
Msa0408370 AT3G25820.1 28.000 350 202 8 29 339 98 436 1.24e-31 125
Msa0408370 AT3G25830.1 28.000 350 202 8 29 339 98 436 1.24e-31 125
Msa0408370 AT3G25810.1 26.453 344 216 8 29 339 99 438 2.06e-29 119
Msa0408370 AT5G23960.2 24.932 365 221 9 9 338 46 392 1.92e-28 115
Msa0408370 AT5G23960.1 24.932 365 223 9 9 338 46 394 1.95e-28 115
Msa0408370 AT3G25820.2 26.901 342 200 8 29 331 98 428 2.50e-27 111
Msa0408370 AT1G70080.1 25.806 279 193 5 71 339 183 457 3.97e-25 106
Msa0408370 AT4G16740.4 26.220 328 197 7 9 302 56 372 3.83e-24 102
Msa0408370 AT4G16740.3 26.220 328 197 7 9 302 56 372 3.83e-24 102
Msa0408370 AT3G14490.1 22.006 359 249 7 10 339 92 448 1.26e-23 102
Msa0408370 AT3G14520.1 20.000 360 250 6 8 339 101 450 2.28e-21 95.5
Msa0408370 AT5G48110.1 32.571 175 112 3 168 339 18 189 6.14e-20 89.7
Msa0408370 AT3G29410.2 23.235 340 227 9 29 339 11 345 1.67e-19 89.7
Msa0408370 AT3G29410.1 23.235 340 227 9 29 339 116 450 2.46e-19 89.4
Msa0408370 AT3G14540.2 19.783 369 240 6 8 339 98 447 5.90e-19 88.2
Msa0408370 AT1G33750.1 21.229 358 249 7 10 339 98 450 6.95e-19 88.2
Msa0408370 AT3G14540.1 19.783 369 240 6 8 339 98 447 7.61e-19 87.8
Msa0408370 AT1G31950.2 19.444 360 240 6 18 339 106 453 1.28e-18 87.0
Msa0408370 AT1G31950.3 20.339 354 244 5 18 339 106 453 1.36e-18 87.0
Msa0408370 AT1G31950.1 20.339 354 244 5 18 339 105 452 1.42e-18 87.0
Msa0408370 AT3G25820.4 26.715 277 153 8 29 266 98 363 5.75e-18 84.7
Msa0408370 AT3G25820.3 26.715 277 153 8 29 266 98 363 5.75e-18 84.7
Msa0408370 AT2G23230.2 24.928 345 226 9 25 339 105 446 9.14e-18 84.7
Msa0408370 AT2G23230.1 24.198 343 226 8 25 339 105 441 9.16e-17 81.6
Msa0408370 AT3G32030.1 20.054 369 243 12 10 339 95 450 4.84e-15 76.6
Msa0408370 AT4G15870.1 24.561 285 184 8 25 281 117 398 7.26e-15 75.9

Find 56 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGGTTTGTCAAGATTTA+TGG 0.092924 3_2:-48131987 Msa0408370:intron
GAAGATGGTCTCAATGATTT+AGG 0.178561 3_2:-48132106 Msa0408370:CDS
GACCATTTCAGCAAAATTAT+TGG 0.211226 3_2:+48131161 None:intergenic
TATTTACAGAAGCTGTTAAT+AGG 0.296508 3_2:-48131424 Msa0408370:intron
GAGGCTCATTGGCTGAATTC+TGG 0.327673 3_2:-48130917 Msa0408370:CDS
CAAAGAAACTTTCATGAAGT+TGG 0.330290 3_2:+48131203 None:intergenic
CTTCTCCTTCAATACTTAGT+TGG 0.335760 3_2:+48132125 None:intergenic
CTTGACAAACCATAGTGAAT+AGG 0.338294 3_2:+48131995 None:intergenic
CTTCAATACCCTATTCACTA+TGG 0.354611 3_2:-48132004 Msa0408370:CDS
TTCTCCTTCAATACTTAGTT+GGG 0.360234 3_2:+48132126 None:intergenic
CTTTCTTGAAAGAGGCTCAT+TGG 0.375504 3_2:-48130928 Msa0408370:CDS
CATATTCATCAATGATTTGA+AGG 0.384746 3_2:-48131957 Msa0408370:CDS
GCCAAGGAGATGAAGTTTGC+AGG 0.387170 3_2:-48131599 Msa0408370:CDS
AGTGTTCAATGCCAAGCCTT+TGG 0.393705 3_2:+48133575 None:intergenic
ATAATATAAACAAGAGAGAT+TGG 0.396389 3_2:+48131491 None:intergenic
ATTAAATGCTTTCTTGAAAG+AGG 0.400182 3_2:-48130936 Msa0408370:CDS
AAAGAAACTTTCATGAAGTT+GGG 0.408850 3_2:+48131204 None:intergenic
TGGCTACTAACGAAATCAAT+TGG 0.412017 3_2:+48133493 None:intergenic
TTGACAAACCATAGTGAATA+GGG 0.413942 3_2:+48131996 None:intergenic
AAATCATTGAGAGTAAAATA+TGG 0.425108 3_2:-48132187 Msa0408370:CDS
ATTTGAAGGCTAAGAACAAA+TGG 0.457215 3_2:-48131943 Msa0408370:CDS
CCCTGCAAACTTCATCTCCT+TGG 0.463904 3_2:+48131598 None:intergenic
AAGATGGTCTCAATGATTTA+GGG 0.471621 3_2:-48132105 Msa0408370:CDS
GCATCCCAACTAAGTATTGA+AGG 0.471653 3_2:-48132130 Msa0408370:CDS
CAGGGTACAATCCTCTGAAA+TGG 0.472535 3_2:-48131580 Msa0408370:CDS
CTAAGTATTGAAGGAGAAGA+TGG 0.480587 3_2:-48132121 Msa0408370:CDS
ATTGACATCATCCAAAGGCT+TGG 0.485610 3_2:-48133586 Msa0408370:CDS
CTTACACATCAAAGCTTGCT+TGG 0.488498 3_2:+48133663 None:intergenic
GTTGTGAGCTTCTTCAAGCA+TGG 0.495898 3_2:-48132075 Msa0408370:CDS
GTTCATGAACTGAGTTATGC+TGG 0.497239 3_2:+48131889 None:intergenic
AAGAGCCACTTTGATCTCCT+CGG 0.504444 3_2:+48133546 None:intergenic
AAACATATATAGCTTGGCTA+TGG 0.510587 3_2:+48132035 None:intergenic
ATCAAATGAAAATTCTCAGT+TGG 0.512307 3_2:+48133607 None:intergenic
ACCCTTTCGTTCGAAAAGCA+TGG 0.515766 3_2:+48131527 None:intergenic
TTTCGATGTTCACGGAACAT+TGG 0.523216 3_2:-48131459 Msa0408370:CDS
ATAAACAAGAGAGATTGGTT+TGG 0.523296 3_2:+48131496 None:intergenic
GGAAGACCTTGGACTGGCCA+AGG 0.524821 3_2:-48131615 Msa0408370:CDS
TGTTTGAAACATATATAGCT+TGG 0.529765 3_2:+48132029 None:intergenic
TGGATCCGTAAAACATGCCA+TGG 0.534660 3_2:+48131547 None:intergenic
TAGTTGGGATGCTTCATACA+AGG 0.535663 3_2:+48132141 None:intergenic
CAATTCAGATGGGAAATGAA+TGG 0.540526 3_2:-48131240 Msa0408370:intron
TCATCTCCTTGGCCAGTCCA+AGG 0.564304 3_2:+48131609 None:intergenic
TTCCTGAATTGATAGATGGT+GGG 0.570853 3_2:-48131636 Msa0408370:intron
GATAGATGGTGGGAAGACCT+TGG 0.578924 3_2:-48131626 Msa0408370:intron
ATTTGATTGACATCATCCAA+AGG 0.587022 3_2:-48133591 Msa0408370:CDS
CCAAGGAGATGAAGTTTGCA+GGG 0.604048 3_2:-48131598 Msa0408370:CDS
ATCCTCTGAAATGGTACATG+TGG 0.610340 3_2:-48131571 Msa0408370:CDS
GGCCACATGTACCATTTCAG+AGG 0.612528 3_2:+48131569 None:intergenic
ATTGAGAGTAAAATATGGTG+AGG 0.627693 3_2:-48132182 Msa0408370:CDS
AAATATGGTGAGGATGTGAA+AGG 0.628992 3_2:-48132172 Msa0408370:CDS
ATGGTGGGAAGACCTTGGAC+TGG 0.644827 3_2:-48131621 Msa0408370:CDS
GGATCCGTAAAACATGCCAT+GGG 0.664006 3_2:+48131548 None:intergenic
CTTGAAGAAGCTCACAACTG+AGG 0.664155 3_2:+48132080 None:intergenic
CATTGAACACTACTTTGCCG+AGG 0.665299 3_2:-48133563 Msa0408370:CDS
CTTTGCCGAGGAGATCAAAG+TGG 0.670162 3_2:-48133551 Msa0408370:CDS
GAAATGGTACATGTGGCCCA+TGG 0.691281 3_2:-48131564 Msa0408370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAAAAAAAAAATTATCTT+TGG - chr3_2:48131560-48131579 Msa0408370:CDS 10.0%
!! TGAAAAAAAAAATTATCTTT+GGG - chr3_2:48131561-48131580 Msa0408370:CDS 10.0%
!!! TTTTTAAAATATGATTAACT+AGG + chr3_2:48133050-48133069 None:intergenic 10.0%
!! AATTATGAAATAAGTGAATA+TGG + chr3_2:48131685-48131704 None:intergenic 15.0%
!!! AATTCTGAAAACAAAATTAT+TGG - chr3_2:48131701-48131720 Msa0408370:intron 15.0%
!!! TTTTTTATTGAGTGATTTTT+TGG + chr3_2:48132557-48132576 None:intergenic 15.0%
!!! AAATGTATTTTGTTTACAAT+TGG - chr3_2:48132579-48132598 Msa0408370:intron 15.0%
!!! ATATTTTTAGTGTATCTATA+GGG + chr3_2:48133711-48133730 None:intergenic 15.0%
!! TATAGATACACTAAAAATAT+CGG - chr3_2:48133711-48133730 Msa0408370:intron 15.0%
!!! ATCCTTTAAAATGAATAAGT+GGG + chr3_2:48131518-48131537 None:intergenic 20.0%
!! GATGTAATTGATATAATACA+AGG + chr3_2:48131749-48131768 None:intergenic 20.0%
!! AACAAAAATTAGAAGTTTAG+TGG + chr3_2:48131822-48131841 None:intergenic 20.0%
!!! ACTAAACTTCTAATTTTTGT+TGG - chr3_2:48131821-48131840 Msa0408370:intron 20.0%
!!! AATTTTTGTTGGTTATATTC+AGG - chr3_2:48131832-48131851 Msa0408370:intron 20.0%
!!! TTTTTTTCGTAGTGATATTT+TGG + chr3_2:48132040-48132059 None:intergenic 20.0%
!!! ATTTTACTCTCAATGATTTT+TGG + chr3_2:48132638-48132657 None:intergenic 20.0%
!! AAATCATTGAGAGTAAAATA+TGG - chr3_2:48132639-48132658 Msa0408370:intron 20.0%
!! GATAAATAATATTGAGTAGA+TGG - chr3_2:48133073-48133092 Msa0408370:intron 20.0%
!! TCAATTTACGATCAAAATTA+TGG - chr3_2:48133101-48133120 Msa0408370:intron 20.0%
!! ATAATATAAACAAGAGAGAT+TGG + chr3_2:48133338-48133357 None:intergenic 20.0%
!!! GATATTTTTAGTGTATCTAT+AGG + chr3_2:48133712-48133731 None:intergenic 20.0%
! TATATCTTAAATAATGCACG+TGG + chr3_2:48130897-48130916 None:intergenic 25.0%
! ATTAGAAATGATGATATGTG+AGG + chr3_2:48130925-48130944 None:intergenic 25.0%
!!! TTAATTTCAGTTTTCAAGCA+TGG - chr3_2:48130996-48131015 Msa0408370:intron 25.0%
! ATCAAATGAAAATTCTCAGT+TGG + chr3_2:48131222-48131241 None:intergenic 25.0%
!! TTCCCACTTATTCATTTTAA+AGG - chr3_2:48131513-48131532 Msa0408370:CDS 25.0%
!!! CATCCTTTAAAATGAATAAG+TGG + chr3_2:48131519-48131538 None:intergenic 25.0%
!!! CACTTATTCATTTTAAAGGA+TGG - chr3_2:48131517-48131536 Msa0408370:CDS 25.0%
! TAGTATACGTTTACTTTATG+TGG + chr3_2:48131859-48131878 None:intergenic 25.0%
! AAAAAAACGTGATTTAGTGA+AGG - chr3_2:48132053-48132072 Msa0408370:CDS 25.0%
! GAAAAGTTATATACACTATC+CGG - chr3_2:48132124-48132143 Msa0408370:CDS 25.0%
! ATAATAAAGATCATAACGAC+CGG + chr3_2:48132146-48132165 None:intergenic 25.0%
! CATTCATTAAATGATGTATG+TGG + chr3_2:48132172-48132191 None:intergenic 25.0%
!! TCATTAAATTCACACTGATT+AGG + chr3_2:48132291-48132310 None:intergenic 25.0%
! TAATCAGTGTGAATTTAATG+AGG - chr3_2:48132290-48132309 Msa0408370:intron 25.0%
!! TGTTTGAAACATATATAGCT+TGG + chr3_2:48132800-48132819 None:intergenic 25.0%
! CATATTCATCAATGATTTGA+AGG - chr3_2:48132869-48132888 Msa0408370:intron 25.0%
!! TTTTAGTCAGAAAACATACT+TGG + chr3_2:48132985-48133004 None:intergenic 25.0%
! TTATTTCCTGAATTGATAGA+TGG - chr3_2:48133186-48133205 Msa0408370:intron 25.0%
! TATTTACAGAAGCTGTTAAT+AGG - chr3_2:48133402-48133421 Msa0408370:intron 25.0%
! TATGTTTAACTCAATTCAGA+TGG - chr3_2:48133575-48133594 Msa0408370:CDS 25.0%
! ATGTTTAACTCAATTCAGAT+GGG - chr3_2:48133576-48133595 Msa0408370:CDS 25.0%
! AAAGAAACTTTCATGAAGTT+GGG + chr3_2:48133625-48133644 None:intergenic 25.0%
!! TACCAATAATTTTGCTGAAA+TGG - chr3_2:48133663-48133682 Msa0408370:CDS 25.0%
!!! TTCTAGCTTAACTAAGATTA+TGG - chr3_2:48133771-48133790 Msa0408370:intron 25.0%
! ATTAAATGCTTTCTTGAAAG+AGG - chr3_2:48133890-48133909 Msa0408370:intron 25.0%
! AGAAAAACTGACTTTACTCA+AGG + chr3_2:48130966-48130985 None:intergenic 30.0%
! TCAATCTGTTCTACATTTTC+AGG - chr3_2:48131128-48131147 Msa0408370:CDS 30.0%
ATTTGATTGACATCATCCAA+AGG - chr3_2:48131235-48131254 Msa0408370:CDS 30.0%
TTGGTTATATTCTCTTTGGT+CGG - chr3_2:48131422-48131441 Msa0408370:intron 30.0%
AAGTTAGTGACTGAAATTAG+TGG - chr3_2:48131941-48131960 Msa0408370:CDS 30.0%
AGTTAGTGACTGAAATTAGT+GGG - chr3_2:48131942-48131961 Msa0408370:CDS 30.0%
CTAACAAACACAATAAGACA+TGG - chr3_2:48131972-48131991 Msa0408370:CDS 30.0%
TTTAGTGAAGGAAATTAGCA+AGG - chr3_2:48132065-48132084 Msa0408370:CDS 30.0%
! AATTTTCGTCACTAATACGT+AGG - chr3_2:48132355-48132374 Msa0408370:intron 30.0%
!! AGCGGTGATATATTTTAAGA+TGG - chr3_2:48132383-48132402 Msa0408370:intron 30.0%
ATTGAGAGTAAAATATGGTG+AGG - chr3_2:48132644-48132663 Msa0408370:intron 30.0%
TTCTCCTTCAATACTTAGTT+GGG + chr3_2:48132703-48132722 None:intergenic 30.0%
AAGATGGTCTCAATGATTTA+GGG - chr3_2:48132721-48132740 Msa0408370:intron 30.0%
AAACATATATAGCTTGGCTA+TGG + chr3_2:48132794-48132813 None:intergenic 30.0%
TTGACAAACCATAGTGAATA+GGG + chr3_2:48132833-48132852 None:intergenic 30.0%
! CTATGGTTTGTCAAGATTTA+TGG - chr3_2:48132839-48132858 Msa0408370:intron 30.0%
ATTTGAAGGCTAAGAACAAA+TGG - chr3_2:48132883-48132902 Msa0408370:intron 30.0%
TCAGGAAATAAATTCGTTAG+TGG + chr3_2:48133177-48133196 None:intergenic 30.0%
! ATAAACAAGAGAGATTGGTT+TGG + chr3_2:48133333-48133352 None:intergenic 30.0%
! GATGATATTTTCGATGTTCA+CGG - chr3_2:48133359-48133378 Msa0408370:intron 30.0%
CAAAGAAACTTTCATGAAGT+TGG + chr3_2:48133626-48133645 None:intergenic 30.0%
GACCATTTCAGCAAAATTAT+TGG + chr3_2:48133668-48133687 None:intergenic 30.0%
ACAAACTTTGTATGAAACAG+TGG - chr3_2:48133859-48133878 Msa0408370:intron 30.0%
CAAACTTTGTATGAAACAGT+GGG - chr3_2:48133860-48133879 Msa0408370:intron 30.0%
!! TCTGTTCTACATTTTCAGGA+TGG - chr3_2:48131132-48131151 Msa0408370:CDS 35.0%
TGGCTACTAACGAAATCAAT+TGG + chr3_2:48131336-48131355 None:intergenic 35.0%
GTGCAACTTCATAAAGTTCA+TGG + chr3_2:48131356-48131375 None:intergenic 35.0%
!! CAGGTTGGTTATATTCTCTT+TGG - chr3_2:48131418-48131437 Msa0408370:intron 35.0%
CGAAATTTATCCCACTCTAA+CGG - chr3_2:48132323-48132342 Msa0408370:intron 35.0%
TTAGTGACGAAAATTGTGAC+AGG + chr3_2:48132350-48132369 None:intergenic 35.0%
AAATATGGTGAGGATGTGAA+AGG - chr3_2:48132654-48132673 Msa0408370:intron 35.0%
CTTCTCCTTCAATACTTAGT+TGG + chr3_2:48132704-48132723 None:intergenic 35.0%
!! CTAAGTATTGAAGGAGAAGA+TGG - chr3_2:48132705-48132724 Msa0408370:intron 35.0%
GAAGATGGTCTCAATGATTT+AGG - chr3_2:48132720-48132739 Msa0408370:intron 35.0%
CTTCAATACCCTATTCACTA+TGG - chr3_2:48132822-48132841 Msa0408370:intron 35.0%
CTTGACAAACCATAGTGAAT+AGG + chr3_2:48132834-48132853 None:intergenic 35.0%
! TGCTGATTATCGGGAAAATA+TGG + chr3_2:48133148-48133167 None:intergenic 35.0%
TTTCCTGAATTGATAGATGG+TGG - chr3_2:48133189-48133208 Msa0408370:intron 35.0%
TTCCTGAATTGATAGATGGT+GGG - chr3_2:48133190-48133209 Msa0408370:intron 35.0%
AATCTCACTTATCTTGATGC+AGG + chr3_2:48133487-48133506 None:intergenic 35.0%
CAATTCAGATGGGAAATGAA+TGG - chr3_2:48133586-48133605 Msa0408370:CDS 35.0%
GAAATGGTCTACAAAAAGCA+TGG - chr3_2:48133679-48133698 Msa0408370:CDS 35.0%
TATGGATTTGAATCTAGCCT+TGG - chr3_2:48133789-48133808 Msa0408370:intron 35.0%
TGCAGTAGCGTTAAAACTAT+TGG - chr3_2:48133815-48133834 Msa0408370:intron 35.0%
GCAGTAGCGTTAAAACTATT+GGG - chr3_2:48133816-48133835 Msa0408370:intron 35.0%
! CTTACACATCAAAGCTTGCT+TGG + chr3_2:48131166-48131185 None:intergenic 40.0%
ATTGACATCATCCAAAGGCT+TGG - chr3_2:48131240-48131259 Msa0408370:intron 40.0%
! ACCAACCTGCATTGACATAA+TGG + chr3_2:48131407-48131426 None:intergenic 40.0%
TGCCAACTTAACTATGCTCA+CGG + chr3_2:48131451-48131470 None:intergenic 40.0%
CAGATATGTTGCCGTATGTA+GGG - chr3_2:48131469-48131488 Msa0408370:CDS 40.0%
TCTTTCGTCGCAACTAGTAA+GGG + chr3_2:48132099-48132118 None:intergenic 40.0%
TTCTTTCGTCGCAACTAGTA+AGG + chr3_2:48132100-48132119 None:intergenic 40.0%
ACTAATACGTAGGATGCTAG+CGG - chr3_2:48132365-48132384 Msa0408370:intron 40.0%
TAGTTGGGATGCTTCATACA+AGG + chr3_2:48132688-48132707 None:intergenic 40.0%
!! GCATCCCAACTAAGTATTGA+AGG - chr3_2:48132696-48132715 Msa0408370:intron 40.0%
GTTCATGAACTGAGTTATGC+TGG + chr3_2:48132940-48132959 None:intergenic 40.0%
! TATGATTAACTAGGTGTCGC+AGG + chr3_2:48133041-48133060 None:intergenic 40.0%
! TGGTCTCTTTGCTGATTATC+GGG + chr3_2:48133157-48133176 None:intergenic 40.0%
! GTGGTCTCTTTGCTGATTAT+CGG + chr3_2:48133158-48133177 None:intergenic 40.0%
TTCCCACCATCTATCAATTC+AGG + chr3_2:48133195-48133214 None:intergenic 40.0%
!! ATCCTCTGAAATGGTACATG+TGG - chr3_2:48133255-48133274 Msa0408370:intron 40.0%
! ATCCATGCTTTTCGAACGAA+AGG - chr3_2:48133297-48133316 Msa0408370:intron 40.0%
! TCCATGCTTTTCGAACGAAA+GGG - chr3_2:48133298-48133317 Msa0408370:intron 40.0%
TTTCGATGTTCACGGAACAT+TGG - chr3_2:48133367-48133386 Msa0408370:intron 40.0%
CTTTCTTGAAAGAGGCTCAT+TGG - chr3_2:48133898-48133917 Msa0408370:intron 40.0%
! TGGCATTTTACCAACAACTG+AGG - chr3_2:48133929-48133948 Msa0408370:intron 40.0%
AGTGTTCAATGCCAAGCCTT+TGG + chr3_2:48131254-48131273 None:intergenic 45.0%
CATTGAACACTACTTTGCCG+AGG - chr3_2:48131263-48131282 Msa0408370:intron 45.0%
! AAGAGCCACTTTGATCTCCT+CGG + chr3_2:48131283-48131302 None:intergenic 45.0%
GCCATTATGTCAATGCAGGT+TGG - chr3_2:48131403-48131422 Msa0408370:intron 45.0%
CTCCGTGAGCATAGTTAAGT+TGG - chr3_2:48131446-48131465 Msa0408370:CDS 45.0%
GCAGATATGTTGCCGTATGT+AGG - chr3_2:48131468-48131487 Msa0408370:CDS 45.0%
TGAACTCTGAGCCCTACATA+CGG + chr3_2:48131483-48131502 None:intergenic 45.0%
CTTGAAGAAGCTCACAACTG+AGG + chr3_2:48132749-48132768 None:intergenic 45.0%
GTTGTGAGCTTCTTCAAGCA+TGG - chr3_2:48132751-48132770 Msa0408370:intron 45.0%
CCAAGGAGATGAAGTTTGCA+GGG - chr3_2:48133228-48133247 Msa0408370:intron 45.0%
! CAGGGTACAATCCTCTGAAA+TGG - chr3_2:48133246-48133265 Msa0408370:intron 45.0%
GGATCCGTAAAACATGCCAT+GGG + chr3_2:48133281-48133300 None:intergenic 45.0%
TGGATCCGTAAAACATGCCA+TGG + chr3_2:48133282-48133301 None:intergenic 45.0%
ACCCTTTCGTTCGAAAAGCA+TGG + chr3_2:48133302-48133321 None:intergenic 45.0%
TTAACGCTACTGCATGTCCA+AGG + chr3_2:48133809-48133828 None:intergenic 45.0%
!!! TTTATTATTTTTTTTTTTTG+AGG - chr3_2:48131031-48131050 Msa0408370:intron 5.0%
!!! TTATTATTTTTTTTTTTTGA+GGG - chr3_2:48131032-48131051 Msa0408370:intron 5.0%
CTTTGCCGAGGAGATCAAAG+TGG - chr3_2:48131275-48131294 Msa0408370:intron 50.0%
GGAGGCCATTATGTCAATGC+AGG - chr3_2:48131399-48131418 Msa0408370:intron 50.0%
TGTGACAGGACCGTTAGAGT+GGG + chr3_2:48132336-48132355 None:intergenic 50.0%
TTGTGACAGGACCGTTAGAG+TGG + chr3_2:48132337-48132356 None:intergenic 50.0%
!! GATAGATGGTGGGAAGACCT+TGG - chr3_2:48133200-48133219 Msa0408370:intron 50.0%
GCCAAGGAGATGAAGTTTGC+AGG - chr3_2:48133227-48133246 Msa0408370:intron 50.0%
CCCTGCAAACTTCATCTCCT+TGG + chr3_2:48133231-48133250 None:intergenic 50.0%
GGCCACATGTACCATTTCAG+AGG + chr3_2:48133260-48133279 None:intergenic 50.0%
GAAATGGTACATGTGGCCCA+TGG - chr3_2:48133262-48133281 Msa0408370:intron 50.0%
! GTGGCCCATGGCATGTTTTA+CGG - chr3_2:48133274-48133293 Msa0408370:intron 50.0%
! GAGGCTCATTGGCTGAATTC+TGG - chr3_2:48133909-48133928 Msa0408370:intron 50.0%
GCATTCCGCTTGCTCAGACA+AGG - chr3_2:48131378-48131397 Msa0408370:intron 55.0%
TTCCGCTTGCTCAGACAAGG+AGG - chr3_2:48131381-48131400 Msa0408370:intron 55.0%
! ATGGTGGGAAGACCTTGGAC+TGG - chr3_2:48133205-48133224 Msa0408370:intron 55.0%
TCATCTCCTTGGCCAGTCCA+AGG + chr3_2:48133220-48133239 None:intergenic 55.0%
GGCCTCCTTGTCTGAGCAAG+CGG + chr3_2:48131386-48131405 None:intergenic 60.0%
!! GGAAGACCTTGGACTGGCCA+AGG - chr3_2:48133211-48133230 Msa0408370:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 48130889 48133959 48130889 ID=Msa0408370;Name=Msa0408370
chr3_2 mRNA 48130889 48133959 48130889 ID=Msa0408370-mRNA-1;Parent=Msa0408370;Name=Msa0408370-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0.5|0.28|0.85|0.33|0.28|7|0|339
chr3_2 exon 48130889 48130969 48130889 ID=Msa0408370-mRNA-1:exon:9326;Parent=Msa0408370-mRNA-1
chr3_2 exon 48131116 48131254 48131116 ID=Msa0408370-mRNA-1:exon:9325;Parent=Msa0408370-mRNA-1
chr3_2 exon 48131425 48131643 48131425 ID=Msa0408370-mRNA-1:exon:9324;Parent=Msa0408370-mRNA-1
chr3_2 exon 48131863 48131994 48131863 ID=Msa0408370-mRNA-1:exon:9323;Parent=Msa0408370-mRNA-1
chr3_2 exon 48132001 48132238 48132001 ID=Msa0408370-mRNA-1:exon:9322;Parent=Msa0408370-mRNA-1
chr3_2 exon 48133500 48133698 48133500 ID=Msa0408370-mRNA-1:exon:9321;Parent=Msa0408370-mRNA-1
chr3_2 exon 48133951 48133959 48133951 ID=Msa0408370-mRNA-1:exon:9320;Parent=Msa0408370-mRNA-1
chr3_2 CDS 48133951 48133959 48133951 ID=Msa0408370-mRNA-1:cds;Parent=Msa0408370-mRNA-1
chr3_2 CDS 48133500 48133698 48133500 ID=Msa0408370-mRNA-1:cds;Parent=Msa0408370-mRNA-1
chr3_2 CDS 48132001 48132238 48132001 ID=Msa0408370-mRNA-1:cds;Parent=Msa0408370-mRNA-1
chr3_2 CDS 48131863 48131994 48131863 ID=Msa0408370-mRNA-1:cds;Parent=Msa0408370-mRNA-1
chr3_2 CDS 48131425 48131643 48131425 ID=Msa0408370-mRNA-1:cds;Parent=Msa0408370-mRNA-1
chr3_2 CDS 48131116 48131254 48131116 ID=Msa0408370-mRNA-1:cds;Parent=Msa0408370-mRNA-1
chr3_2 CDS 48130889 48130969 48130889 ID=Msa0408370-mRNA-1:cds;Parent=Msa0408370-mRNA-1
Gene Sequence

>Msa0408370

ATGAACCACGATGGTATTTATGCCAAGCAAGCTTTGATGTGTAAGCAAGTTAACAAGAAACTTGCTACTAGTGAAGATCCAACTGAGAATTTTCATTTGATTGACATCATCCAAAGGCTTGGCATTGAACACTACTTTGCCGAGGAGATCAAAGTGGCTCTTGAAAAACAATTTTTGATATTGAGCAGCAATCCAATTGATTTCGTTAAGTTATTTGACTGCTTGAAGTGTAGCCAAAAATCATTGAGAGTAAAATATGGTGAGGATGTGAAAGGTCTCATTGCCTTGTATGAAGCATCCCAACTAAGTATTGAAGGAGAAGATGGTCTCAATGATTTAGGGCACCTCAGTTGTGAGCTTCTTCAAGCATGGCTTTCAAGACATCAAGACCATAGCCAAGCTATATATGTTTCAAACACTCTTCAATACCCTATTCACTATGGTTTATTTATGGATAAAAGCATATTCATCAATGATTTGAAGGCTAAGAACAAATGGATCTGTTTAGATGAACTTGCAAAAATCAACTCCAGCATAACTCAGTTCATGAACAAAAATGAGAGCGTTGAAGTTTCCAAATGGTGGGAAGACCTTGGACTGGCCAAGGAGATGAAGTTTGCAGGGTACAATCCTCTGAAATGGTACATGTGGCCCATGGCATGTTTTACGGATCCATGCTTTTCGAACGAAAGGGTTGAGCTCACCAAACCAATCTCTCTTGTTTATATTATTGATGATATTTTCGATGTTCACGGAACATTGGATCAACTTACTCTATTTACAGAAGCTGTTAATAGATGGGAAATGAATGGTGCAGAGAATCTTCCCAACTTCATGAAAGTTTCTTTGAGTTCTCTTTACAAAGTTACCAATAATTTTGCTGAAATGGTCTACAAAAAGCATGGATTCAACCCTATAGATACACTAAAAATATCGTGGGTGCGCTTATTAAATGCTTTCTTGAAAGAGGCTCATTGGCTGAATTCTGGCATTTTACCAACAACTGAGGAATACTTG
Protein sequence

>Msa0408370

MNHDGIYAKQALMCKQVNKKLATSEDPTENFHLIDIIQRLGIEHYFAEEIKVALEKQFLILSSNPIDFVKLFDCLKCSQKSLRVKYGEDVKGLIALYEASQLSIEGEDGLNDLGHLSCELLQAWLSRHQDHSQAIYVSNTLQYPIHYGLFMDKSIFINDLKAKNKWICLDELAKINSSITQFMNKNESVEVSKWWEDLGLAKEMKFAGYNPLKWYMWPMACFTDPCFSNERVELTKPISLVYIIDDIFDVHGTLDQLTLFTEAVNRWEMNGAENLPNFMKVSLSSLYKVTNNFAEMVYKKHGFNPIDTLKISWVRLLNAFLKEAHWLNSGILPTTEEYL