AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0421770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0421770 MtrunA17_Chr3g0122411 94.183 447 13 1 1 447 1 434 0.0 863
Msa0421770 MtrunA17_Chr8g0377421 47.748 333 128 5 1 325 1 295 2.09e-86 266
Msa0421770 MtrunA17_Chr8g0377401 55.061 247 88 5 1 237 1 234 1.17e-68 219
Msa0421770 MtrunA17_Chr8g0377431 47.748 222 94 6 1 218 1 204 4.64e-48 164
Msa0421770 MtrunA17_Chr5g0420051 41.827 208 112 4 39 240 52 256 4.05e-40 149
Msa0421770 MtrunA17_Chr7g0231911 48.447 161 79 3 93 249 73 233 5.36e-38 140
Msa0421770 MtrunA17_Chr7g0258471 36.897 290 123 9 120 387 91 342 1.08e-36 139
Msa0421770 MtrunA17_Chr8g0360361 70.000 80 24 0 163 242 63 142 5.84e-36 130
Msa0421770 MtrunA17_Chr3g0132091 49.606 127 61 3 151 276 58 182 1.52e-35 134
Msa0421770 MtrunA17_Chr4g0061651 50.781 128 56 2 117 240 74 198 2.84e-35 136
Msa0421770 MtrunA17_Chr4g0046991 55.963 109 32 1 130 238 54 146 9.50e-35 126
Msa0421770 MtrunA17_Chr8g0334671 48.684 152 63 3 118 261 147 291 4.80e-34 130
Msa0421770 MtrunA17_Chr7g0224821 59.341 91 34 1 154 244 69 156 8.74e-33 127
Msa0421770 MtrunA17_Chr8g0386271 56.311 103 42 2 164 266 98 197 1.68e-32 127
Msa0421770 MtrunA17_Chr2g0282521 57.895 95 39 1 148 242 48 141 3.86e-32 119
Msa0421770 MtrunA17_Chr2g0308321 60.000 90 36 0 151 240 157 246 8.72e-31 124
Msa0421770 MtrunA17_Chr1g0194121 56.842 95 41 0 150 244 132 226 3.04e-29 121
Msa0421770 MtrunA17_Chr2g0302791 47.934 121 55 2 134 247 102 221 1.40e-28 119
Msa0421770 MtrunA17_Chr1g0162871 58.621 87 36 0 163 249 102 188 2.28e-28 118
Msa0421770 MtrunA17_Chr8g0377391 47.531 162 74 6 262 412 1 162 5.53e-28 108
Msa0421770 MtrunA17_Chr7g0270541 61.250 80 31 0 165 244 145 224 2.17e-26 112
Msa0421770 MtrunA17_Chr2g0286661 48.315 89 46 0 150 238 88 176 7.72e-22 98.6
Msa0421770 MtrunA17_Chr2g0317041 56.452 62 27 0 150 211 84 145 2.71e-19 84.7
Msa0421770 MtrunA17_Chr1g0175031 46.602 103 49 2 115 211 18 120 9.36e-19 82.4
Msa0421770 MtrunA17_Chr8g0377411 50.000 98 41 3 346 438 3 97 7.67e-14 67.4
Msa0421770 MtrunA17_Chr1g0175021 33.721 172 94 5 212 381 8 161 6.40e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0421770 AT5G43270.3 39.031 392 167 16 1 366 1 346 7.29e-55 188
Msa0421770 AT5G43270.1 39.031 392 167 16 1 366 1 346 7.29e-55 188
Msa0421770 AT5G43270.2 39.031 392 167 16 1 366 1 346 7.29e-55 188
Msa0421770 AT1G27370.6 47.674 258 110 7 1 240 1 251 1.65e-53 182
Msa0421770 AT1G27370.1 47.674 258 110 7 1 240 1 251 5.90e-53 182
Msa0421770 AT1G27370.5 47.674 258 110 7 1 240 1 251 5.90e-53 182
Msa0421770 AT1G27370.3 47.674 258 110 7 1 240 1 251 5.90e-53 182
Msa0421770 AT1G27370.2 47.674 258 110 7 1 240 1 251 5.90e-53 182
Msa0421770 AT1G27370.4 47.674 258 110 7 1 240 1 251 5.90e-53 182
Msa0421770 AT1G27370.7 47.674 258 110 7 1 240 1 251 5.90e-53 182
Msa0421770 AT1G27360.2 38.402 388 200 14 1 366 1 371 1.26e-51 179
Msa0421770 AT1G27360.1 38.402 388 200 14 1 366 1 371 1.26e-51 179
Msa0421770 AT1G27360.3 38.402 388 200 14 1 366 1 371 1.26e-51 179
Msa0421770 AT1G27360.4 38.402 388 200 14 1 366 1 371 1.26e-51 179
Msa0421770 AT2G42200.1 38.996 259 122 10 120 371 32 261 1.23e-35 135
Msa0421770 AT1G69170.1 66.304 92 31 0 148 239 107 198 1.85e-35 136
Msa0421770 AT3G57920.1 53.279 122 44 3 120 241 27 135 3.55e-34 131
Msa0421770 AT1G02065.1 53.279 122 49 2 118 238 147 261 7.15e-33 127
Msa0421770 AT5G50570.1 60.976 82 32 0 163 244 99 180 1.80e-31 124
Msa0421770 AT5G50570.2 60.976 82 32 0 163 244 99 180 1.80e-31 124
Msa0421770 AT5G50670.1 60.976 82 32 0 163 244 99 180 1.80e-31 124
Msa0421770 AT5G50670.2 60.976 82 32 0 163 244 99 180 1.80e-31 124
Msa0421770 AT2G33810.1 59.091 88 33 1 151 238 43 127 1.04e-30 115
Msa0421770 AT1G76580.2 60.674 89 35 0 163 251 112 200 2.64e-30 124
Msa0421770 AT1G76580.3 60.674 89 35 0 163 251 112 200 2.96e-30 124
Msa0421770 AT1G76580.1 60.674 89 35 0 163 251 112 200 5.23e-30 124
Msa0421770 AT3G60030.1 61.250 80 31 0 165 244 127 206 3.57e-29 121
Msa0421770 AT1G53160.3 59.494 79 32 0 165 243 54 132 1.22e-28 111
Msa0421770 AT2G47070.1 60.215 93 36 1 165 256 106 198 1.40e-28 119
Msa0421770 AT1G53160.2 45.385 130 56 4 165 288 54 174 1.56e-28 111
Msa0421770 AT1G53160.1 45.385 130 56 4 165 288 54 174 1.56e-28 111
Msa0421770 AT1G20980.1 60.000 95 37 1 151 244 106 200 3.99e-28 118
Msa0421770 AT3G15270.1 60.811 74 29 0 165 238 63 136 5.41e-28 109
Msa0421770 AT1G02065.2 46.875 96 43 2 118 212 147 235 5.94e-19 86.3
Msa0421770 AT5G18830.2 51.316 76 36 1 165 239 138 213 5.09e-18 87.4
Msa0421770 AT5G18830.3 51.316 76 36 1 165 239 138 213 5.70e-18 87.4
Msa0421770 AT5G18830.1 51.316 76 36 1 165 239 138 213 6.16e-18 87.0
Msa0421770 AT2G47070.2 60.294 68 26 1 190 256 2 69 2.38e-16 82.0

Find 171 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCTCTTTACACTCTTT+TGG 0.140706 3_2:+70793342 Msa0421770:five_prime_UTR
GGTTTAGCTTGATTGGTTTC+TGG 0.141242 3_2:+70793699 Msa0421770:five_prime_UTR
TTTGGATCAGACAAGGTATT+TGG 0.193506 3_2:-70796407 None:intergenic
TTCCAACTAAACACAGATTT+TGG 0.195484 3_2:-70793786 None:intergenic
GCTAAATTTGAAGTATTCTT+TGG 0.209568 3_2:-70796034 None:intergenic
TGCAGAGGAAGAGGCTTTCT+TGG 0.222637 3_2:-70795071 None:intergenic
TTGATATTGGCTTTCCATTA+TGG 0.227294 3_2:+70793536 Msa0421770:five_prime_UTR
CAAGCTTTCATGATGACTTT+TGG 0.235533 3_2:+70793270 Msa0421770:five_prime_UTR
AGCTTGCTGTTGGGCATGTC+TGG 0.241731 3_2:-70796061 None:intergenic
CTGTGATAAATGTGAATTAT+TGG 0.244929 3_2:+70793883 Msa0421770:five_prime_UTR
TCATGCAAATATTAAATTAC+TGG 0.261187 3_2:+70794155 Msa0421770:five_prime_UTR
GGTTTCTGGCATTTATGATA+TGG 0.273991 3_2:+70793713 Msa0421770:five_prime_UTR
GGATTCAATTTCACTGCTTC+AGG 0.274502 3_2:-70795493 None:intergenic
GAGCATAAAATCTTTGGTTT+GGG 0.292731 3_2:-70796086 None:intergenic
TTTGGGTTTATCATATTCTA+AGG 0.293376 3_2:+70793360 Msa0421770:five_prime_UTR
CCATTATGGTTTAAAACTTC+AGG 0.298202 3_2:+70793550 Msa0421770:five_prime_UTR
TCCAAACATGTACTCATTAT+AGG 0.303289 3_2:+70794062 Msa0421770:five_prime_UTR
TGAATTAGACATCATACTTA+TGG 0.306180 3_2:+70794120 Msa0421770:five_prime_UTR
ACCTATAATGAGTACATGTT+TGG 0.321644 3_2:-70794063 None:intergenic
TTTAGACGATTCTTTCTTAC+CGG 0.323854 3_2:-70794951 None:intergenic
TGAGCATAAAATCTTTGGTT+TGG 0.329729 3_2:-70796087 None:intergenic
GCTGAGAAAGCTTGAGATAA+AGG 0.330586 3_2:+70793394 Msa0421770:five_prime_UTR
TTAACATCTTGTGTAGCATT+TGG 0.347549 3_2:-70796425 None:intergenic
GCAAGGGAAACTTGACTCAT+AGG 0.354069 3_2:-70796687 None:intergenic
AATCTTGGTTCCTTTGCCTT+TGG 0.356006 3_2:-70796176 None:intergenic
TTGTGGCCTCGACCTCTCTT+TGG 0.373974 3_2:+70795168 Msa0421770:CDS
AAGTTTAGGTAGTGCATGTT+TGG 0.375544 3_2:+70793934 Msa0421770:five_prime_UTR
CTCTTCTGTCAACCAATTCA+TGG 0.377885 3_2:+70796459 Msa0421770:CDS
TACATCTTAAATGCTGGCTT+TGG 0.377938 3_2:-70793624 None:intergenic
TACTACCACCACAGGCGATT+TGG 0.381836 3_2:-70795233 None:intergenic
TAGAAGAGTCTCTTTCCTTA+AGG 0.384235 3_2:-70793499 None:intergenic
TTCTTACCGGTGGAATTGTC+TGG 0.385894 3_2:-70794938 None:intergenic
TGATATGGAGGTTTGGTTGT+TGG 0.388695 3_2:+70793728 Msa0421770:five_prime_UTR
ACATGCACCTGCTACATTGC+TGG 0.389565 3_2:-70795279 None:intergenic
ATTGATAGAATACATATTAC+AGG 0.392874 3_2:+70796374 Msa0421770:CDS
GCATGTTTGGATTGGAGGGT+GGG 0.395614 3_2:+70793947 Msa0421770:five_prime_UTR
AATCTCCCGGGCAATGGGAC+TGG 0.397861 3_2:+70794678 Msa0421770:CDS
GCTTATGTTGCAACCGGAAA+AGG 0.401245 3_2:-70793248 None:intergenic
GCTGCTGGAGAAGTTTCAAT+TGG 0.402606 3_2:-70794980 None:intergenic
CCATGTTGAAAGTGAAGTTT+AGG 0.402615 3_2:+70793920 Msa0421770:five_prime_UTR
GCATTTATGATATGGAGGTT+TGG 0.408107 3_2:+70793721 Msa0421770:five_prime_UTR
AGCTGCCAGGTGGAAGGTTG+TGG 0.411491 3_2:+70795151 Msa0421770:CDS
ATGTACTAGCAAGGTCCTTA+AGG 0.412487 3_2:+70793484 Msa0421770:five_prime_UTR
ATCTCCCGGGCAATGGGACT+GGG 0.414643 3_2:+70794679 Msa0421770:CDS
CAGCGCCTGTATCTCGTTCT+CGG 0.419065 3_2:+70796616 Msa0421770:CDS
AATTTCACTGCTTCAGGCTG+AGG 0.421915 3_2:-70795487 None:intergenic
ACAATCTACTGATTGGAGTA+TGG 0.424631 3_2:+70794748 Msa0421770:CDS
TGCATGTTTGGATTGGAGGG+TGG 0.426451 3_2:+70793946 Msa0421770:five_prime_UTR
CGTGAAATCTCCCGGGCAAT+GGG 0.426658 3_2:+70794673 Msa0421770:CDS
CAGCATTTAAGATGTAGAAT+AGG 0.427718 3_2:+70793631 Msa0421770:five_prime_UTR
CAATATTCCAGTGACGAAAA+AGG 0.427818 3_2:-70796566 None:intergenic
GTGAGCAATGGTTCTGCTGC+TGG 0.432022 3_2:-70794995 None:intergenic
GTTGAGGTTGTTGTTAATGT+GGG 0.433390 3_2:+70793830 Msa0421770:five_prime_UTR
GTATTCTTTGGAAAAGCAAA+TGG 0.434089 3_2:-70796022 None:intergenic
ACAACCTGGGACAGCAATCT+TGG 0.439368 3_2:-70796191 None:intergenic
AAGTTTCCCTTGCAACCAGC+TGG 0.440887 3_2:+70796697 Msa0421770:CDS
TAGTGCATGTTTGGATTGGA+GGG 0.445875 3_2:+70793943 Msa0421770:five_prime_UTR
AAGCTTGCTTATGTTGCAAC+CGG 0.446076 3_2:-70793254 None:intergenic
GAATTAGACATCATACTTAT+GGG 0.446634 3_2:+70794121 Msa0421770:five_prime_UTR
ATACACTCCAATCGGACAAC+CGG 0.451604 3_2:+70796512 Msa0421770:CDS
TACTTTGAGGATGTTAGCAC+TGG 0.451656 3_2:+70795040 Msa0421770:CDS
TCTTCTGTCAACCAATTCAT+GGG 0.452114 3_2:+70796460 Msa0421770:CDS
CGAGATACAGGCGCTGGAGC+TGG 0.455430 3_2:-70796609 None:intergenic
TGTTTGGATTGGAGGGTGGG+TGG 0.460095 3_2:+70793950 Msa0421770:five_prime_UTR
CATCAAACTCTGAGAGATCA+TGG 0.461380 3_2:-70795408 None:intergenic
ACATTTATCACAGCAATGTC+AGG 0.466045 3_2:-70793873 None:intergenic
CAACCTTCCACCTGGCAGCT+TGG 0.467365 3_2:-70795148 None:intergenic
TGTTGAGGTTGTTGTTAATG+TGG 0.470186 3_2:+70793829 Msa0421770:five_prime_UTR
TCGCCTGTGGTGGTAGTAGC+TGG 0.470263 3_2:+70795238 Msa0421770:CDS
GTAGTGCATGTTTGGATTGG+AGG 0.471021 3_2:+70793942 Msa0421770:five_prime_UTR
GTTGGGATGTTGATTCCATC+TGG 0.471449 3_2:+70794791 Msa0421770:CDS
CCTTTCTTTCCGCACAAAAG+AGG 0.471950 3_2:-70795097 None:intergenic
TAGGTAGTGCATGTTTGGAT+TGG 0.472523 3_2:+70793939 Msa0421770:five_prime_UTR
CTATTCTACATCTTAAATGC+TGG 0.472880 3_2:-70793630 None:intergenic
GTAATTTAATATTTGCATGA+AGG 0.476058 3_2:-70794152 None:intergenic
ACAGGTTACAATCTACTGAT+TGG 0.476801 3_2:+70794741 Msa0421770:CDS
TGGTAAAAGATTCTACTTTG+AGG 0.477123 3_2:+70795027 Msa0421770:CDS
AGGGGTGCCGGTTGTCCGAT+TGG 0.477616 3_2:-70796519 None:intergenic
TGTGGTGGTAGTAGCTGGTT+TGG 0.481417 3_2:+70795243 Msa0421770:CDS
TGCCAAAATCTGTGTTTAGT+TGG 0.483513 3_2:+70793784 Msa0421770:five_prime_UTR
ACGTGAAATCTCCCGGGCAA+TGG 0.485685 3_2:+70794672 Msa0421770:CDS
TAATTTAATATTTGCATGAA+GGG 0.486333 3_2:-70794151 None:intergenic
GAAGATCGAGAAATCAATGT+TGG 0.486543 3_2:+70794773 Msa0421770:CDS
AAGAGGAACTGCAGAGGAAG+AGG 0.487764 3_2:-70795080 None:intergenic
CCTAAACTTCACTTTCAACA+TGG 0.491187 3_2:-70793920 None:intergenic
ATCATATGGACCCCATGAAT+TGG 0.492799 3_2:-70796471 None:intergenic
TTGTCCGATTGGAGTGTATG+AGG 0.494492 3_2:-70796508 None:intergenic
AGCATACTCATTAATCCAGC+TGG 0.497222 3_2:-70796712 None:intergenic
GATTGGAGTGTATGAGGGAG+GGG 0.501793 3_2:-70796502 None:intergenic
CTTTGCCTTTGGATATAAAG+TGG 0.501899 3_2:-70796165 None:intergenic
GAGTGGAACGTGAAATCTCC+CGG 0.503280 3_2:+70794665 Msa0421770:CDS
CATTAATCCAGCTGGTTGCA+AGG 0.507260 3_2:-70796704 None:intergenic
TTGATTCCATCTGGTGGTAG+TGG 0.508737 3_2:+70794800 Msa0421770:CDS
AAGATCGAGAAATCAATGTT+GGG 0.508795 3_2:+70794774 Msa0421770:CDS
CACAAATTCTATGTTTCCGA+TGG 0.510237 3_2:-70795201 None:intergenic
TAATGTGGGGACTCATTCAA+TGG 0.511610 3_2:+70793844 Msa0421770:five_prime_UTR
CAAGGGAAACTTGACTCATA+GGG 0.517421 3_2:-70796686 None:intergenic
CATGCTAAATCCAAGCTGCC+AGG 0.528294 3_2:+70795138 Msa0421770:CDS
GTAACCCGAGAACGAGATAC+AGG 0.528459 3_2:-70796621 None:intergenic
CGATTGGAGTGTATGAGGGA+GGG 0.531040 3_2:-70796503 None:intergenic
TTCTGGCATTTATGATATGG+AGG 0.531477 3_2:+70793716 Msa0421770:five_prime_UTR
TGCAGATGGGAGGCAGACAA+TGG 0.534432 3_2:+70795997 Msa0421770:intron
CCTCCCTCATACACTCCAAT+CGG 0.535964 3_2:+70796504 Msa0421770:CDS
TATTCTTTGGAAAAGCAAAT+GGG 0.538218 3_2:-70796021 None:intergenic
AGCGCCTGTATCTCGTTCTC+GGG 0.540985 3_2:+70796617 Msa0421770:CDS
ACATCAATGTTAACTCAAGA+AGG 0.548574 3_2:+70794911 Msa0421770:CDS
GGAACCAAGATTGCTGTCCC+AGG 0.548599 3_2:+70796187 Msa0421770:CDS
GCAGATGGGAGGCAGACAAT+GGG 0.549297 3_2:+70795998 Msa0421770:intron
CAAAAGCAGCAGAAACTCAC+AGG 0.550624 3_2:+70794723 Msa0421770:CDS
GGTAAGAAAGAATCGTCTAA+AGG 0.551763 3_2:+70794953 Msa0421770:CDS
TTGGCACACTGATCAACATC+AGG 0.552387 3_2:+70796586 Msa0421770:CDS
TTTGGATATAAAGTGGCCAC+TGG 0.552682 3_2:-70796158 None:intergenic
AGTTGCATACTGAGGGGTGC+CGG 0.554723 3_2:-70796531 None:intergenic
CCAAGCTTTAGTGTGAGCAA+TGG 0.557830 3_2:-70795007 None:intergenic
AGCTCAAATCTAACAAAGGA+AGG 0.557965 3_2:-70793656 None:intergenic
GGGGACGAAGAGAGAGCTGA+TGG 0.559258 3_2:-70795514 None:intergenic
CTAAACTTCACTTTCAACAT+GGG 0.559810 3_2:-70793919 None:intergenic
AAAGAGAGGAAGAAGATCAC+AGG 0.559961 3_2:-70793330 None:intergenic
ATTGGAGTGTATGAGGGAGG+GGG 0.561383 3_2:-70796501 None:intergenic
AGAATACTTCAAATTTAGCA+TGG 0.561391 3_2:+70796038 Msa0421770:CDS
GAATAATTCATCATCAAATG+AGG 0.562543 3_2:+70794880 Msa0421770:CDS
ATTAATCCAGCTGGTTGCAA+GGG 0.568989 3_2:-70796703 None:intergenic
CGCTGAGCAGTTGCATACTG+AGG 0.570454 3_2:-70796539 None:intergenic
AATCCAAGCTGCCAGGTGGA+AGG 0.571632 3_2:+70795145 Msa0421770:CDS
CTTTAGCCAAAGAGAGGTCG+AGG 0.573405 3_2:-70795174 None:intergenic
TGTGAATTATTGGAATCTGA+TGG 0.578849 3_2:+70793893 Msa0421770:five_prime_UTR
CGAGGCCACAACCTTCCACC+TGG 0.580184 3_2:-70795156 None:intergenic
CTTGAGATTTATACCATAGG+GGG 0.580458 3_2:-70795533 None:intergenic
AGACGATTCTTTCTTACCGG+TGG 0.582162 3_2:-70794948 None:intergenic
TTAAAGCTCAAATCTAACAA+AGG 0.586460 3_2:-70793660 None:intergenic
AGTGGCCACTTTATATCCAA+AGG 0.587042 3_2:+70796160 Msa0421770:CDS
CCATTGCTCACACTAAAGCT+TGG 0.588106 3_2:+70795007 Msa0421770:CDS
CTTTGGCTAAAGATTACCAT+CGG 0.588534 3_2:+70795185 Msa0421770:CDS
TGTCCGATTGGAGTGTATGA+GGG 0.588850 3_2:-70796507 None:intergenic
GTCTTTCAGATCACAATGCA+AGG 0.591028 3_2:+70795455 Msa0421770:CDS
GCATAAAATCTTTGGTTTGG+GGG 0.594228 3_2:-70796084 None:intergenic
AATATGTGATTCATTTGTTG+AGG 0.594917 3_2:+70793814 Msa0421770:five_prime_UTR
GCTGAGCAGTTGCATACTGA+GGG 0.602329 3_2:-70796538 None:intergenic
TTGACAGAAGAGAGAGAGCA+CGG 0.603893 3_2:-70796448 None:intergenic
AGCATAAAATCTTTGGTTTG+GGG 0.604215 3_2:-70796085 None:intergenic
CCGATTGGAGTGTATGAGGG+AGG 0.604444 3_2:-70796504 None:intergenic
TTGAGGTTGTTGTTAATGTG+GGG 0.607836 3_2:+70793831 Msa0421770:five_prime_UTR
CACTTTATATCCAAAGGCAA+AGG 0.613225 3_2:+70796166 Msa0421770:CDS
ATCACAGCAATGTCAGGAGT+CGG 0.614030 3_2:-70793867 None:intergenic
CGAGAACGAGATACAGGCGC+TGG 0.614056 3_2:-70796615 None:intergenic
AAACCAGCTACTACCACCAC+AGG 0.617364 3_2:-70795241 None:intergenic
TTGCATTGTGATCTGAAAGA+CGG 0.621589 3_2:-70795453 None:intergenic
TGTGAGGACTTGTCATGGAG+TGG 0.623994 3_2:+70794648 Msa0421770:exon
GAATTTGTGAGGACTTGTCA+TGG 0.630777 3_2:+70794643 Msa0421770:exon
GAATAGCCACTACCACCAGA+TGG 0.631021 3_2:-70794806 None:intergenic
TCATTCCAAATCGCCTGTGG+TGG 0.632811 3_2:+70795228 Msa0421770:CDS
CTTCTGTCAACCAATTCATG+GGG 0.634971 3_2:+70796461 Msa0421770:CDS
AGTGGAACGTGAAATCTCCC+GGG 0.641060 3_2:+70794666 Msa0421770:CDS
ACCCAAAAGAGTGTAAAGAG+AGG 0.641317 3_2:-70793344 None:intergenic
TAGTGAGTTTGACAAAATCA+CGG 0.643134 3_2:-70794035 None:intergenic
ATCTACTGATTGGAGTATGG+AGG 0.643141 3_2:+70794751 Msa0421770:CDS
TTGGAGTGTATGAGGGAGGG+GGG 0.645515 3_2:-70796500 None:intergenic
GGGATGTTGATTCCATCTGG+TGG 0.647072 3_2:+70794794 Msa0421770:CDS
CAGTCATTCCAAATCGCCTG+TGG 0.648152 3_2:+70795225 Msa0421770:CDS
TGTAGCATTTGGATCAGACA+AGG 0.648361 3_2:-70796414 None:intergenic
ATATTCATTCTGCAGATGGG+AGG 0.649420 3_2:+70795987 Msa0421770:intron
GGTTCTCCAGACAATTCCAC+CGG 0.652614 3_2:+70794932 Msa0421770:CDS
TCTTGAGATTTATACCATAG+GGG 0.653113 3_2:-70795534 None:intergenic
GGTAATCTTTAGCCAAAGAG+AGG 0.662641 3_2:-70795180 None:intergenic
CTGAGCAGTTGCATACTGAG+GGG 0.662729 3_2:-70796537 None:intergenic
GCTAAATCCAAGCTGCCAGG+TGG 0.663879 3_2:+70795141 Msa0421770:CDS
ATGCTATCTGATGATTCCAG+TGG 0.666446 3_2:+70796142 Msa0421770:CDS
TAATGCATGCTTCATCCTCG+AGG 0.674783 3_2:+70794840 Msa0421770:CDS
CATCTGTATATGAATTTGTG+AGG 0.689586 3_2:+70794632 Msa0421770:five_prime_UTR
GCACAAAAGAGGAACTGCAG+AGG 0.694164 3_2:-70795086 None:intergenic
AAAAGAAAATGACAATGACA+TGG 0.723335 3_2:+70793084 Msa0421770:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACTTTTATTTTTATTTTTG+TGG + chr3_2:70793131-70793150 Msa0421770:five_prime_UTR 10.0%
!!! TGATTTTCTTTTTCTTTTTT+GGG + chr3_2:70793107-70793126 Msa0421770:five_prime_UTR 15.0%
!!! TACATAATAGATTTTGATAT+TGG + chr3_2:70793523-70793542 Msa0421770:five_prime_UTR 15.0%
!!! ATTTTTTTTTTGGTACAATT+TGG - chr3_2:70793577-70793596 None:intergenic 15.0%
!!! GGTACAAAATATTTTTTTTT+TGG - chr3_2:70793587-70793606 None:intergenic 15.0%
!! TAATTTAATATTTGCATGAA+GGG - chr3_2:70794154-70794173 None:intergenic 15.0%
!!! TTGGTTTGTTTAAAATAAAT+TGG + chr3_2:70795349-70795368 Msa0421770:intron 15.0%
!!! ACACTTAATATTTTCTTATA+AGG - chr3_2:70795875-70795894 None:intergenic 15.0%
!!! ATTAAATTTAGTTTGTTCAA+TGG + chr3_2:70796255-70796274 Msa0421770:intron 15.0%
!!! GTGATTTTCTTTTTCTTTTT+TGG + chr3_2:70793106-70793125 Msa0421770:five_prime_UTR 20.0%
!!! GTTATTATTATTTCCTTTTC+CGG + chr3_2:70793235-70793254 Msa0421770:five_prime_UTR 20.0%
!!! CAAATGTTTCTGTTTTTTTA+GGG + chr3_2:70793437-70793456 Msa0421770:five_prime_UTR 20.0%
!!! TTTAAAAAAGATGCTTTTTG+AGG + chr3_2:70793678-70793697 Msa0421770:five_prime_UTR 20.0%
!! GTAATTTAATATTTGCATGA+AGG - chr3_2:70794155-70794174 None:intergenic 20.0%
!! TCATGCAAATATTAAATTAC+TGG + chr3_2:70794155-70794174 Msa0421770:five_prime_UTR 20.0%
!!! GTAAACATTTTACATTAGTA+GGG + chr3_2:70794177-70794196 Msa0421770:intron 20.0%
!! AGAATTTATACAGATATACT+TGG + chr3_2:70794453-70794472 Msa0421770:intron 20.0%
!! AACTATACAAGATAATAACA+AGG - chr3_2:70794544-70794563 None:intergenic 20.0%
!! AACTACTAGTCAAATAATAA+TGG - chr3_2:70794599-70794618 None:intergenic 20.0%
!!! AACTTAAACTATGATTTTGA+AGG + chr3_2:70795383-70795402 Msa0421770:intron 20.0%
!! TTATGAGAGAATTATAAGAA+TGG + chr3_2:70795655-70795674 Msa0421770:intron 20.0%
!!! TTAGAATTTCTTTTGAGAAA+TGG - chr3_2:70795695-70795714 None:intergenic 20.0%
!!! TACTCTATGACTATATTTTT+GGG - chr3_2:70795801-70795820 None:intergenic 20.0%
!!! ATACTCTATGACTATATTTT+TGG - chr3_2:70795802-70795821 None:intergenic 20.0%
!! TTTAATAAGAAATCATGACT+CGG - chr3_2:70796241-70796260 None:intergenic 20.0%
!! ATTGATAGAATACATATTAC+AGG + chr3_2:70796374-70796393 Msa0421770:CDS 20.0%
! AAAAGAAAATGACAATGACA+TGG + chr3_2:70793084-70793103 Msa0421770:five_prime_UTR 25.0%
!!! AAAAGGCATCTTTTTTTCAT+GGG - chr3_2:70793301-70793320 None:intergenic 25.0%
!!! AAAAAGGCATCTTTTTTTCA+TGG - chr3_2:70793302-70793321 None:intergenic 25.0%
! ATCACAGGAAGAAAAAAAAA+AGG - chr3_2:70793318-70793337 None:intergenic 25.0%
!! TTTGGGTTTATCATATTCTA+AGG + chr3_2:70793360-70793379 Msa0421770:five_prime_UTR 25.0%
!!! GCAAATGTTTCTGTTTTTTT+AGG + chr3_2:70793436-70793455 Msa0421770:five_prime_UTR 25.0%
!!! TTCTGTTTTTTTAGGGTAAT+AGG + chr3_2:70793444-70793463 Msa0421770:five_prime_UTR 25.0%
!!! TCTGTTTTTTTAGGGTAATA+GGG + chr3_2:70793445-70793464 Msa0421770:five_prime_UTR 25.0%
! CTAGTACATATAAAAAGCTT+TGG - chr3_2:70793473-70793492 None:intergenic 25.0%
! TTAAAGCTCAAATCTAACAA+AGG - chr3_2:70793663-70793682 None:intergenic 25.0%
!! AATATGTGATTCATTTGTTG+AGG + chr3_2:70793814-70793833 Msa0421770:five_prime_UTR 25.0%
! CTGTGATAAATGTGAATTAT+TGG + chr3_2:70793883-70793902 Msa0421770:five_prime_UTR 25.0%
!! GTAGCTTTTGCAAAAATTTA+CGG + chr3_2:70794005-70794024 Msa0421770:five_prime_UTR 25.0%
! TGAATTAGACATCATACTTA+TGG + chr3_2:70794120-70794139 Msa0421770:five_prime_UTR 25.0%
! GAATTAGACATCATACTTAT+GGG + chr3_2:70794121-70794140 Msa0421770:five_prime_UTR 25.0%
!! GGTAAACATTTTACATTAGT+AGG + chr3_2:70794176-70794195 Msa0421770:intron 25.0%
!! ATTTTACATTAGTAGGGTTT+AGG + chr3_2:70794183-70794202 Msa0421770:intron 25.0%
!! TTTTACATTAGTAGGGTTTA+GGG + chr3_2:70794184-70794203 Msa0421770:intron 25.0%
! AAAATACCCTTGTAATTCTT+TGG + chr3_2:70794227-70794246 Msa0421770:intron 25.0%
! GAATAATTCATCATCAAATG+AGG + chr3_2:70794880-70794899 Msa0421770:CDS 25.0%
! TTTAAACAAACCAAAAAGAC+AGG - chr3_2:70795343-70795362 None:intergenic 25.0%
! TTCTTGAGATTTATACCATA+GGG - chr3_2:70795538-70795557 None:intergenic 25.0%
! ATTCTTGAGATTTATACCAT+AGG - chr3_2:70795539-70795558 None:intergenic 25.0%
! TTCAGTTTCTTTGTAAGAAA+TGG + chr3_2:70795821-70795840 Msa0421770:intron 25.0%
! TTTAATATTCATTCTGCAGA+TGG + chr3_2:70795983-70796002 Msa0421770:intron 25.0%
! TTAATATTCATTCTGCAGAT+GGG + chr3_2:70795984-70796003 Msa0421770:intron 25.0%
!! TATTCTTTGGAAAAGCAAAT+GGG - chr3_2:70796024-70796043 None:intergenic 25.0%
!! GCTAAATTTGAAGTATTCTT+TGG - chr3_2:70796037-70796056 None:intergenic 25.0%
! AGAATACTTCAAATTTAGCA+TGG + chr3_2:70796038-70796057 Msa0421770:CDS 25.0%
!!! AGTTTTGAGCATAAAATCTT+TGG - chr3_2:70796095-70796114 None:intergenic 25.0%
!!! TAACAGTTGTTTCTACAAAT+AGG - chr3_2:70796349-70796368 None:intergenic 25.0%
!! TATTTTTGTGGATGTGTGAT+TGG + chr3_2:70793143-70793162 Msa0421770:five_prime_UTR 30.0%
!!! ATGAGTGCTATTTTTGTTGA+AGG + chr3_2:70793196-70793215 Msa0421770:five_prime_UTR 30.0%
!!! TGAGTGCTATTTTTGTTGAA+GGG + chr3_2:70793197-70793216 Msa0421770:five_prime_UTR 30.0%
!!! CTGTTTTTTTAGGGTAATAG+GGG + chr3_2:70793446-70793465 Msa0421770:five_prime_UTR 30.0%
! GCTTTTTATATGTACTAGCA+AGG + chr3_2:70793475-70793494 Msa0421770:five_prime_UTR 30.0%
!! TTGATATTGGCTTTCCATTA+TGG + chr3_2:70793536-70793555 Msa0421770:five_prime_UTR 30.0%
!!! CCTGAAGTTTTAAACCATAA+TGG - chr3_2:70793553-70793572 None:intergenic 30.0%
! CCATTATGGTTTAAAACTTC+AGG + chr3_2:70793550-70793569 Msa0421770:five_prime_UTR 30.0%
TTGTACCAAAAAAACACTTC+AGG + chr3_2:70793600-70793619 Msa0421770:five_prime_UTR 30.0%
CTATTCTACATCTTAAATGC+TGG - chr3_2:70793633-70793652 None:intergenic 30.0%
! CAGCATTTAAGATGTAGAAT+AGG + chr3_2:70793631-70793650 Msa0421770:five_prime_UTR 30.0%
!!! TTTTTGAGGTTTAGCTTGAT+TGG + chr3_2:70793692-70793711 Msa0421770:five_prime_UTR 30.0%
! TTCCAACTAAACACAGATTT+TGG - chr3_2:70793789-70793808 None:intergenic 30.0%
TGTGAATTATTGGAATCTGA+TGG + chr3_2:70793893-70793912 Msa0421770:five_prime_UTR 30.0%
CTAAACTTCACTTTCAACAT+GGG - chr3_2:70793922-70793941 None:intergenic 30.0%
TAGTGAGTTTGACAAAATCA+CGG - chr3_2:70794038-70794057 None:intergenic 30.0%
TCCAAACATGTACTCATTAT+AGG + chr3_2:70794062-70794081 Msa0421770:five_prime_UTR 30.0%
ACCTATAATGAGTACATGTT+TGG - chr3_2:70794066-70794085 None:intergenic 30.0%
TAGTTGTAGTAGAACTATCT+TGG + chr3_2:70794267-70794286 Msa0421770:intron 30.0%
TATAATCCATGTCTTGCTTT+AGG + chr3_2:70794349-70794368 Msa0421770:intron 30.0%
TTTAGGAATTAGCAGTTCAT+AGG + chr3_2:70794366-70794385 Msa0421770:intron 30.0%
!!! TCGTACTGTTTTTAAGTGAT+TGG + chr3_2:70794425-70794444 Msa0421770:intron 30.0%
CATCTGTATATGAATTTGTG+AGG + chr3_2:70794632-70794651 Msa0421770:five_prime_UTR 30.0%
AAGATCGAGAAATCAATGTT+GGG + chr3_2:70794774-70794793 Msa0421770:CDS 30.0%
ACATCAATGTTAACTCAAGA+AGG + chr3_2:70794911-70794930 Msa0421770:CDS 30.0%
TTTAGACGATTCTTTCTTAC+CGG - chr3_2:70794954-70794973 None:intergenic 30.0%
TGGTAAAAGATTCTACTTTG+AGG + chr3_2:70795027-70795046 Msa0421770:CDS 30.0%
! ATTGTCAATGTCATTTGCAT+TGG - chr3_2:70795314-70795333 None:intergenic 30.0%
TCTTGAGATTTATACCATAG+GGG - chr3_2:70795537-70795556 None:intergenic 30.0%
! AAGGCTAATTGATCTTTTAG+GGG - chr3_2:70795566-70795585 None:intergenic 30.0%
! CAAGGCTAATTGATCTTTTA+GGG - chr3_2:70795567-70795586 None:intergenic 30.0%
! ACAAGGCTAATTGATCTTTT+AGG - chr3_2:70795568-70795587 None:intergenic 30.0%
TAAACTAATGAAGCAAGACA+AGG - chr3_2:70795585-70795604 None:intergenic 30.0%
TGAATGGCGATATCTTAAAA+AGG + chr3_2:70795632-70795651 Msa0421770:intron 30.0%
TACAACCAATAGCATATCAA+TGG - chr3_2:70795721-70795740 None:intergenic 30.0%
! ATATGCTATTGGTTGTATGA+AGG + chr3_2:70795724-70795743 Msa0421770:intron 30.0%
! GTATTCTTTGGAAAAGCAAA+TGG - chr3_2:70796025-70796044 None:intergenic 30.0%
!! AGCATAAAATCTTTGGTTTG+GGG - chr3_2:70796088-70796107 None:intergenic 30.0%
! GAGCATAAAATCTTTGGTTT+GGG - chr3_2:70796089-70796108 None:intergenic 30.0%
TGAGCATAAAATCTTTGGTT+TGG - chr3_2:70796090-70796109 None:intergenic 30.0%
!!! ACAAACTTGCTGAAGTTTTT+TGG - chr3_2:70796118-70796137 None:intergenic 30.0%
TTAAGATTAGAGAACAACCT+GGG - chr3_2:70796207-70796226 None:intergenic 30.0%
! TTTAAGATTAGAGAACAACC+TGG - chr3_2:70796208-70796227 None:intergenic 30.0%
TGAAACATGCAATAAACTAG+AGG - chr3_2:70796307-70796326 None:intergenic 30.0%
TTAACATCTTGTGTAGCATT+TGG - chr3_2:70796428-70796447 None:intergenic 30.0%
! CAAGCTTTCATGATGACTTT+TGG + chr3_2:70793270-70793289 Msa0421770:five_prime_UTR 35.0%
! TTCCTCTCTTTACACTCTTT+TGG + chr3_2:70793342-70793361 Msa0421770:five_prime_UTR 35.0%
! TCCTCTCTTTACACTCTTTT+GGG + chr3_2:70793343-70793362 Msa0421770:five_prime_UTR 35.0%
TAGAAGAGTCTCTTTCCTTA+AGG - chr3_2:70793502-70793521 None:intergenic 35.0%
!!! TTGGTCCTGAAGTGTTTTTT+TGG - chr3_2:70793608-70793627 None:intergenic 35.0%
!! TACATCTTAAATGCTGGCTT+TGG - chr3_2:70793627-70793646 None:intergenic 35.0%
AGCTCAAATCTAACAAAGGA+AGG - chr3_2:70793659-70793678 None:intergenic 35.0%
!! GGTTTCTGGCATTTATGATA+TGG + chr3_2:70793713-70793732 Msa0421770:five_prime_UTR 35.0%
TTCTGGCATTTATGATATGG+AGG + chr3_2:70793716-70793735 Msa0421770:five_prime_UTR 35.0%
GCATTTATGATATGGAGGTT+TGG + chr3_2:70793721-70793740 Msa0421770:five_prime_UTR 35.0%
TGCCAAAATCTGTGTTTAGT+TGG + chr3_2:70793784-70793803 Msa0421770:five_prime_UTR 35.0%
! TGTTGAGGTTGTTGTTAATG+TGG + chr3_2:70793829-70793848 Msa0421770:five_prime_UTR 35.0%
! GTTGAGGTTGTTGTTAATGT+GGG + chr3_2:70793830-70793849 Msa0421770:five_prime_UTR 35.0%
! TTGAGGTTGTTGTTAATGTG+GGG + chr3_2:70793831-70793850 Msa0421770:five_prime_UTR 35.0%
ACATTTATCACAGCAATGTC+AGG - chr3_2:70793876-70793895 None:intergenic 35.0%
CCTAAACTTCACTTTCAACA+TGG - chr3_2:70793923-70793942 None:intergenic 35.0%
CCATGTTGAAAGTGAAGTTT+AGG + chr3_2:70793920-70793939 Msa0421770:five_prime_UTR 35.0%
AAGTTTAGGTAGTGCATGTT+TGG + chr3_2:70793934-70793953 Msa0421770:five_prime_UTR 35.0%
TACCCTTGTAATTCTTTGGT+TGG + chr3_2:70794231-70794250 Msa0421770:intron 35.0%
CTCCAACCAAAGAATTACAA+GGG - chr3_2:70794236-70794255 None:intergenic 35.0%
CTTGTAATTCTTTGGTTGGA+GGG + chr3_2:70794235-70794254 Msa0421770:intron 35.0%
TTGTAATTCTTTGGTTGGAG+GGG + chr3_2:70794236-70794255 Msa0421770:intron 35.0%
!! ATCTTGGTTTGCATTTGCAT+AGG + chr3_2:70794283-70794302 Msa0421770:intron 35.0%
CTAATTCCTAAAGCAAGACA+TGG - chr3_2:70794358-70794377 None:intergenic 35.0%
ACAGGTTACAATCTACTGAT+TGG + chr3_2:70794741-70794760 Msa0421770:CDS 35.0%
! ACAATCTACTGATTGGAGTA+TGG + chr3_2:70794748-70794767 Msa0421770:CDS 35.0%
GAAGATCGAGAAATCAATGT+TGG + chr3_2:70794773-70794792 Msa0421770:CDS 35.0%
GGTAAGAAAGAATCGTCTAA+AGG + chr3_2:70794953-70794972 Msa0421770:CDS 35.0%
CTTTGGCTAAAGATTACCAT+CGG + chr3_2:70795185-70795204 Msa0421770:CDS 35.0%
CACAAATTCTATGTTTCCGA+TGG - chr3_2:70795204-70795223 None:intergenic 35.0%
!! CAATTAGCTTCCTGTCTTTT+TGG + chr3_2:70795330-70795349 Msa0421770:intron 35.0%
TTGCATTGTGATCTGAAAGA+CGG - chr3_2:70795456-70795475 None:intergenic 35.0%
CTTGAGATTTATACCATAGG+GGG - chr3_2:70795536-70795555 None:intergenic 35.0%
!! CTAATTGATCTTTTAGGGGA+GGG - chr3_2:70795562-70795581 None:intergenic 35.0%
!!! TTGCCATTTTGTTTCTCGTT+TGG + chr3_2:70795607-70795626 Msa0421770:intron 35.0%
!!! TGCCATTTTGTTTCTCGTTT+GGG + chr3_2:70795608-70795627 Msa0421770:intron 35.0%
ATCCCAAACGAGAAACAAAA+TGG - chr3_2:70795613-70795632 None:intergenic 35.0%
AACCTCCATTGATATGCTAT+TGG + chr3_2:70795713-70795732 Msa0421770:intron 35.0%
AACCAATAGCATATCAATGG+AGG - chr3_2:70795718-70795737 None:intergenic 35.0%
!! GCATAAAATCTTTGGTTTGG+GGG - chr3_2:70796087-70796106 None:intergenic 35.0%
CTTTGCCTTTGGATATAAAG+TGG - chr3_2:70796168-70796187 None:intergenic 35.0%
CACTTTATATCCAAAGGCAA+AGG + chr3_2:70796166-70796185 Msa0421770:CDS 35.0%
! AATAAGAAATCATGACTCGG+AGG - chr3_2:70796238-70796257 None:intergenic 35.0%
!! CAGTTGTTTCTACAAATAGG+AGG - chr3_2:70796346-70796365 None:intergenic 35.0%
! TTTGGATCAGACAAGGTATT+TGG - chr3_2:70796410-70796429 None:intergenic 35.0%
TCTTCTGTCAACCAATTCAT+GGG + chr3_2:70796460-70796479 Msa0421770:CDS 35.0%
CAATATTCCAGTGACGAAAA+AGG - chr3_2:70796569-70796588 None:intergenic 35.0%
!! CTTTTTCGTCACTGGAATAT+TGG + chr3_2:70796567-70796586 Msa0421770:CDS 35.0%
! AAGCTTGCTTATGTTGCAAC+CGG - chr3_2:70793257-70793276 None:intergenic 40.0%
AAAGAGAGGAAGAAGATCAC+AGG - chr3_2:70793333-70793352 None:intergenic 40.0%
! ACCCAAAAGAGTGTAAAGAG+AGG - chr3_2:70793347-70793366 None:intergenic 40.0%
GCTGAGAAAGCTTGAGATAA+AGG + chr3_2:70793394-70793413 Msa0421770:five_prime_UTR 40.0%
ATGTACTAGCAAGGTCCTTA+AGG + chr3_2:70793484-70793503 Msa0421770:five_prime_UTR 40.0%
!! GGTTTAGCTTGATTGGTTTC+TGG + chr3_2:70793699-70793718 Msa0421770:five_prime_UTR 40.0%
! TGATATGGAGGTTTGGTTGT+TGG + chr3_2:70793728-70793747 Msa0421770:five_prime_UTR 40.0%
TAATGTGGGGACTCATTCAA+TGG + chr3_2:70793844-70793863 Msa0421770:five_prime_UTR 40.0%
TAGGTAGTGCATGTTTGGAT+TGG + chr3_2:70793939-70793958 Msa0421770:five_prime_UTR 40.0%
! TAGTGCATGTTTGGATTGGA+GGG + chr3_2:70793943-70793962 Msa0421770:five_prime_UTR 40.0%
CATTAGTAGGGTTTAGGGAT+TGG + chr3_2:70794189-70794208 Msa0421770:intron 40.0%
CCTCCAACCAAAGAATTACA+AGG - chr3_2:70794237-70794256 None:intergenic 40.0%
CCTTGTAATTCTTTGGTTGG+AGG + chr3_2:70794234-70794253 Msa0421770:intron 40.0%
CCAGAGTAATGATTGGAAGA+AGG - chr3_2:70794491-70794510 None:intergenic 40.0%
CCTTCTTCCAATCATTACTC+TGG + chr3_2:70794488-70794507 Msa0421770:intron 40.0%
GCAGAAACCAGAGTAATGAT+TGG - chr3_2:70794498-70794517 None:intergenic 40.0%
GAATTTGTGAGGACTTGTCA+TGG + chr3_2:70794643-70794662 Msa0421770:exon 40.0%
! ATCTACTGATTGGAGTATGG+AGG + chr3_2:70794751-70794770 Msa0421770:CDS 40.0%
! TACTTTGAGGATGTTAGCAC+TGG + chr3_2:70795040-70795059 Msa0421770:CDS 40.0%
GGTAATCTTTAGCCAAAGAG+AGG - chr3_2:70795183-70795202 None:intergenic 40.0%
! CATCAAACTCTGAGAGATCA+TGG - chr3_2:70795411-70795430 None:intergenic 40.0%
GTCTTTCAGATCACAATGCA+AGG + chr3_2:70795455-70795474 Msa0421770:CDS 40.0%
GGATTCAATTTCACTGCTTC+AGG - chr3_2:70795496-70795515 None:intergenic 40.0%
!! GCTAATTGATCTTTTAGGGG+AGG - chr3_2:70795563-70795582 None:intergenic 40.0%
! TGTTTCTCGTTTGGGATGAA+TGG + chr3_2:70795616-70795635 Msa0421770:intron 40.0%
ATATTCATTCTGCAGATGGG+AGG + chr3_2:70795987-70796006 Msa0421770:intron 40.0%
ATGCTATCTGATGATTCCAG+TGG + chr3_2:70796142-70796161 Msa0421770:CDS 40.0%
TTTGGATATAAAGTGGCCAC+TGG - chr3_2:70796161-70796180 None:intergenic 40.0%
! AGTGGCCACTTTATATCCAA+AGG + chr3_2:70796160-70796179 Msa0421770:CDS 40.0%
AATCTTGGTTCCTTTGCCTT+TGG - chr3_2:70796179-70796198 None:intergenic 40.0%
TGTAGCATTTGGATCAGACA+AGG - chr3_2:70796417-70796436 None:intergenic 40.0%
CTCTTCTGTCAACCAATTCA+TGG + chr3_2:70796459-70796478 Msa0421770:CDS 40.0%
CTTCTGTCAACCAATTCATG+GGG + chr3_2:70796461-70796480 Msa0421770:CDS 40.0%
ATCATATGGACCCCATGAAT+TGG - chr3_2:70796474-70796493 None:intergenic 40.0%
CAAGGGAAACTTGACTCATA+GGG - chr3_2:70796689-70796708 None:intergenic 40.0%
GCTTATGTTGCAACCGGAAA+AGG - chr3_2:70793251-70793270 None:intergenic 45.0%
ATCACAGCAATGTCAGGAGT+CGG - chr3_2:70793870-70793889 None:intergenic 45.0%
! GTAGTGCATGTTTGGATTGG+AGG + chr3_2:70793942-70793961 Msa0421770:five_prime_UTR 45.0%
TAATTCTTTGGTTGGAGGGG+TGG + chr3_2:70794239-70794258 Msa0421770:intron 45.0%
CAAAAGCAGCAGAAACTCAC+AGG + chr3_2:70794723-70794742 Msa0421770:CDS 45.0%
!! GTTGGGATGTTGATTCCATC+TGG + chr3_2:70794791-70794810 Msa0421770:CDS 45.0%
TTGATTCCATCTGGTGGTAG+TGG + chr3_2:70794800-70794819 Msa0421770:CDS 45.0%
TAATGCATGCTTCATCCTCG+AGG + chr3_2:70794840-70794859 Msa0421770:CDS 45.0%
TTCTTACCGGTGGAATTGTC+TGG - chr3_2:70794941-70794960 None:intergenic 45.0%
AGACGATTCTTTCTTACCGG+TGG - chr3_2:70794951-70794970 None:intergenic 45.0%
GCTGCTGGAGAAGTTTCAAT+TGG - chr3_2:70794983-70795002 None:intergenic 45.0%
! CCAAGCTTTAGTGTGAGCAA+TGG - chr3_2:70795010-70795029 None:intergenic 45.0%
CCATTGCTCACACTAAAGCT+TGG + chr3_2:70795007-70795026 Msa0421770:CDS 45.0%
! TCTGCAGTTCCTCTTTTGTG+CGG + chr3_2:70795088-70795107 Msa0421770:CDS 45.0%
CCTTTCTTTCCGCACAAAAG+AGG - chr3_2:70795100-70795119 None:intergenic 45.0%
! CCTCTTTTGTGCGGAAAGAA+AGG + chr3_2:70795097-70795116 Msa0421770:CDS 45.0%
! GATTTTGCCAGCAATGTAGC+AGG + chr3_2:70795272-70795291 Msa0421770:CDS 45.0%
AATTTCACTGCTTCAGGCTG+AGG - chr3_2:70795490-70795509 None:intergenic 45.0%
TTGACAGAAGAGAGAGAGCA+CGG - chr3_2:70796451-70796470 None:intergenic 45.0%
TGTCCGATTGGAGTGTATGA+GGG - chr3_2:70796510-70796529 None:intergenic 45.0%
TTGTCCGATTGGAGTGTATG+AGG - chr3_2:70796511-70796530 None:intergenic 45.0%
ATACACTCCAATCGGACAAC+CGG + chr3_2:70796512-70796531 Msa0421770:CDS 45.0%
TTGGCACACTGATCAACATC+AGG + chr3_2:70796586-70796605 Msa0421770:CDS 45.0%
GCAAGGGAAACTTGACTCAT+AGG - chr3_2:70796690-70796709 None:intergenic 45.0%
! TGCATGTTTGGATTGGAGGG+TGG + chr3_2:70793946-70793965 Msa0421770:five_prime_UTR 50.0%
! GCATGTTTGGATTGGAGGGT+GGG + chr3_2:70793947-70793966 Msa0421770:five_prime_UTR 50.0%
GCATTTGCATAGGTGCTAGC+AGG + chr3_2:70794293-70794312 Msa0421770:intron 50.0%
TGTGAGGACTTGTCATGGAG+TGG + chr3_2:70794648-70794667 Msa0421770:exon 50.0%
GAGTGGAACGTGAAATCTCC+CGG + chr3_2:70794665-70794684 Msa0421770:CDS 50.0%
AGTGGAACGTGAAATCTCCC+GGG + chr3_2:70794666-70794685 Msa0421770:CDS 50.0%
!! GGGATGTTGATTCCATCTGG+TGG + chr3_2:70794794-70794813 Msa0421770:CDS 50.0%
GAATAGCCACTACCACCAGA+TGG - chr3_2:70794809-70794828 None:intergenic 50.0%
! AGCAGATTTTGAGCTCCTCG+AGG - chr3_2:70794858-70794877 None:intergenic 50.0%
GGTTCTCCAGACAATTCCAC+CGG + chr3_2:70794932-70794951 Msa0421770:CDS 50.0%
TGCAGAGGAAGAGGCTTTCT+TGG - chr3_2:70795074-70795093 None:intergenic 50.0%
AAGAGGAACTGCAGAGGAAG+AGG - chr3_2:70795083-70795102 None:intergenic 50.0%
GCACAAAAGAGGAACTGCAG+AGG - chr3_2:70795089-70795108 None:intergenic 50.0%
CATGCTAAATCCAAGCTGCC+AGG + chr3_2:70795138-70795157 Msa0421770:CDS 50.0%
CTTTAGCCAAAGAGAGGTCG+AGG - chr3_2:70795177-70795196 None:intergenic 50.0%
CAGTCATTCCAAATCGCCTG+TGG + chr3_2:70795225-70795244 Msa0421770:CDS 50.0%
TCATTCCAAATCGCCTGTGG+TGG + chr3_2:70795228-70795247 Msa0421770:CDS 50.0%
TACTACCACCACAGGCGATT+TGG - chr3_2:70795236-70795255 None:intergenic 50.0%
AAACCAGCTACTACCACCAC+AGG - chr3_2:70795244-70795263 None:intergenic 50.0%
TGTGGTGGTAGTAGCTGGTT+TGG + chr3_2:70795243-70795262 Msa0421770:CDS 50.0%
ACATGCACCTGCTACATTGC+TGG - chr3_2:70795282-70795301 None:intergenic 50.0%
ACAACCTGGGACAGCAATCT+TGG - chr3_2:70796194-70796213 None:intergenic 50.0%
ATTGGAGTGTATGAGGGAGG+GGG - chr3_2:70796504-70796523 None:intergenic 50.0%
GATTGGAGTGTATGAGGGAG+GGG - chr3_2:70796505-70796524 None:intergenic 50.0%
CGATTGGAGTGTATGAGGGA+GGG - chr3_2:70796506-70796525 None:intergenic 50.0%
CCTCCCTCATACACTCCAAT+CGG + chr3_2:70796504-70796523 Msa0421770:CDS 50.0%
CTGAGCAGTTGCATACTGAG+GGG - chr3_2:70796540-70796559 None:intergenic 50.0%
GCTGAGCAGTTGCATACTGA+GGG - chr3_2:70796541-70796560 None:intergenic 50.0%
GTAACCCGAGAACGAGATAC+AGG - chr3_2:70796624-70796643 None:intergenic 50.0%
AAGTTTCCCTTGCAACCAGC+TGG + chr3_2:70796697-70796716 Msa0421770:CDS 50.0%
! TGTTTGGATTGGAGGGTGGG+TGG + chr3_2:70793950-70793969 Msa0421770:five_prime_UTR 55.0%
ACGTGAAATCTCCCGGGCAA+TGG + chr3_2:70794672-70794691 Msa0421770:CDS 55.0%
CGTGAAATCTCCCGGGCAAT+GGG + chr3_2:70794673-70794692 Msa0421770:CDS 55.0%
! TTTTCCCAGTCCCATTGCCC+GGG - chr3_2:70794686-70794705 None:intergenic 55.0%
! GTTTTCCCAGTCCCATTGCC+CGG - chr3_2:70794687-70794706 None:intergenic 55.0%
! GTGAGCAATGGTTCTGCTGC+TGG - chr3_2:70794998-70795017 None:intergenic 55.0%
GCTAAATCCAAGCTGCCAGG+TGG + chr3_2:70795141-70795160 Msa0421770:CDS 55.0%
AATCCAAGCTGCCAGGTGGA+AGG + chr3_2:70795145-70795164 Msa0421770:CDS 55.0%
TTGTGGCCTCGACCTCTCTT+TGG + chr3_2:70795168-70795187 Msa0421770:CDS 55.0%
TGCAGATGGGAGGCAGACAA+TGG + chr3_2:70795997-70796016 Msa0421770:intron 55.0%
GCAGATGGGAGGCAGACAAT+GGG + chr3_2:70795998-70796017 Msa0421770:intron 55.0%
!! AGCTTGCTGTTGGGCATGTC+TGG - chr3_2:70796064-70796083 None:intergenic 55.0%
!! GTTTGGGGGAGCTTGCTGTT+GGG - chr3_2:70796073-70796092 None:intergenic 55.0%
GGAACCAAGATTGCTGTCCC+AGG + chr3_2:70796187-70796206 Msa0421770:CDS 55.0%
! GAGGGGGGCTTGGTATCATA+TGG - chr3_2:70796488-70796507 None:intergenic 55.0%
TTGGAGTGTATGAGGGAGGG+GGG - chr3_2:70796503-70796522 None:intergenic 55.0%
CCGATTGGAGTGTATGAGGG+AGG - chr3_2:70796507-70796526 None:intergenic 55.0%
! AGTTGCATACTGAGGGGTGC+CGG - chr3_2:70796534-70796553 None:intergenic 55.0%
CGCTGAGCAGTTGCATACTG+AGG - chr3_2:70796542-70796561 None:intergenic 55.0%
!! GCGCTCACCTTTTTCGTCAC+TGG + chr3_2:70796559-70796578 Msa0421770:CDS 55.0%
CAGCGCCTGTATCTCGTTCT+CGG + chr3_2:70796616-70796635 Msa0421770:CDS 55.0%
AGCGCCTGTATCTCGTTCTC+GGG + chr3_2:70796617-70796636 Msa0421770:CDS 55.0%
AATCTCCCGGGCAATGGGAC+TGG + chr3_2:70794678-70794697 Msa0421770:CDS 60.0%
ATCTCCCGGGCAATGGGACT+GGG + chr3_2:70794679-70794698 Msa0421770:CDS 60.0%
CAACCTTCCACCTGGCAGCT+TGG - chr3_2:70795151-70795170 None:intergenic 60.0%
AGCTGCCAGGTGGAAGGTTG+TGG + chr3_2:70795151-70795170 Msa0421770:CDS 60.0%
TCGCCTGTGGTGGTAGTAGC+TGG + chr3_2:70795238-70795257 Msa0421770:CDS 60.0%
GGGGACGAAGAGAGAGCTGA+TGG - chr3_2:70795517-70795536 None:intergenic 60.0%
GCTCTCTCTTCGTCCCCCTA+TGG + chr3_2:70795520-70795539 Msa0421770:CDS 60.0%
!! GGTTTGGGGGAGCTTGCTGT+TGG - chr3_2:70796074-70796093 None:intergenic 60.0%
CGAGAACGAGATACAGGCGC+TGG - chr3_2:70796618-70796637 None:intergenic 60.0%
CGAGGCCACAACCTTCCACC+TGG - chr3_2:70795159-70795178 None:intergenic 65.0%
GTGTATGAGGGAGGGGGGCT+TGG - chr3_2:70796498-70796517 None:intergenic 65.0%
!! AGGGGTGCCGGTTGTCCGAT+TGG - chr3_2:70796522-70796541 None:intergenic 65.0%
!! CGAGATACAGGCGCTGGAGC+TGG - chr3_2:70796612-70796631 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 70793058 70796724 70793058 ID=Msa0421770;Name=Msa0421770
chr3_2 mRNA 70793058 70796724 70793058 ID=Msa0421770-mRNA-1;Parent=Msa0421770;Name=Msa0421770-mRNA-1;_AED=0.16;_eAED=0.17;_QI=1164|0.75|0.8|1|0.75|0.6|5|0|447
chr3_2 exon 70793058 70794176 70793058 ID=Msa0421770-mRNA-1:exon:17121;Parent=Msa0421770-mRNA-1
chr3_2 exon 70794617 70795293 70794617 ID=Msa0421770-mRNA-1:exon:17122;Parent=Msa0421770-mRNA-1
chr3_2 exon 70795405 70795541 70795405 ID=Msa0421770-mRNA-1:exon:17123;Parent=Msa0421770-mRNA-1
chr3_2 exon 70796002 70796208 70796002 ID=Msa0421770-mRNA-1:exon:17124;Parent=Msa0421770-mRNA-1
chr3_2 exon 70796357 70796724 70796357 ID=Msa0421770-mRNA-1:exon:17125;Parent=Msa0421770-mRNA-1
chr3_2 five_prime_UTR 70793058 70794176 70793058 ID=Msa0421770-mRNA-1:five_prime_utr;Parent=Msa0421770-mRNA-1
chr3_2 five_prime_UTR 70794617 70794661 70794617 ID=Msa0421770-mRNA-1:five_prime_utr;Parent=Msa0421770-mRNA-1
chr3_2 CDS 70794662 70795293 70794662 ID=Msa0421770-mRNA-1:cds;Parent=Msa0421770-mRNA-1
chr3_2 CDS 70795405 70795541 70795405 ID=Msa0421770-mRNA-1:cds;Parent=Msa0421770-mRNA-1
chr3_2 CDS 70796002 70796208 70796002 ID=Msa0421770-mRNA-1:cds;Parent=Msa0421770-mRNA-1
chr3_2 CDS 70796357 70796724 70796357 ID=Msa0421770-mRNA-1:cds;Parent=Msa0421770-mRNA-1
Gene Sequence

>Msa0421770

ATGGAGTGGAACGTGAAATCTCCCGGGCAATGGGACTGGGAAAACTTATTCTTCTCGAATTCAAAAGCAGCAGAAACTCACAGGTTACAATCTACTGATTGGAGTATGGAGGAAGATCGAGAAATCAATGTTGGGATGTTGATTCCATCTGGTGGTAGTGGCTATTCAGTGTCTAAACTAATGCATGCTTCATCCTCGAGGAGCTCAAAATCTGCTTCGAATAATTCATCATCAAATGAGGACAGCAAGACATCAATGTTAACTCAAGAAGGTTCTCCAGACAATTCCACCGGTAAGAAAGAATCGTCTAAAGGAGATCCAATTGAAACTTCTCCAGCAGCAGAACCATTGCTCACACTAAAGCTTGGTAAAAGATTCTACTTTGAGGATGTTAGCACTGGAAGTCATTCCAAGAAAGCCTCTTCCTCTGCAGTTCCTCTTTTGTGCGGAAAGAAAGGTAAATCGAGCAGTCAGAACATGCTAAATCCAAGCTGCCAGGTGGAAGGTTGTGGCCTCGACCTCTCTTTGGCTAAAGATTACCATCGGAAACATAGAATTTGTGACAGTCATTCCAAATCGCCTGTGGTGGTAGTAGCTGGTTTGGAGCGTCGATTTTGCCAGCAATGTAGCAGGTTCCATGATCTCTCAGAGTTTGATGATAAAAAAAGAAGCTGCAGACGCCGTCTTTCAGATCACAATGCAAGGCGTCGCAAACCTCAGCCTGAAGCAGTGAAATTGAATCCATCAGCTCTCTCTTCGTCCCCCTATGATGGGAGGCAGACAATGGGCCCATTTGCTTTTCCAAAGAATACTTCAAATTTAGCATGGCCAGACATGCCCAACAGCAAGCTCCCCCAAACCAAAGATTTTATGCTCAAAACTCCAAAAAACTTCAGCAAGTTTGTCACTATGCTATCTGATGATTCCAGTGGCCACTTTATATCCAAAGGCAAAGGAACCAAGATTGCTGTCCCAGAAACAACTGTTATTCACATTGATAGAATACATATTACAGGTCTAGAAGATCCAAATACCTTGTCTGATCCAAATGCTACACAAGATGTTAACCGTGCTCTCTCTCTTCTGTCAACCAATTCATGGGGTCCATATGATACCAAGCCCCCCTCCCTCATACACTCCAATCGGACAACCGGCACCCCTCAGTATGCAACTGCTCAGCGCTCACCTTTTTCGTCACTGGAATATTGGCACACTGATCAACATCAGGCCAGCTCCAGCGCCTGTATCTCGTTCTCGGGTTACGACAATAGCAATCGCTTTCAAGACTTTCAGCTGTTCAGCGAACCCTATGAGTCAAGTTTCCCTTGCAACCAGCTGGATTAA
Protein sequence

>Msa0421770

MEWNVKSPGQWDWENLFFSNSKAAETHRLQSTDWSMEEDREINVGMLIPSGGSGYSVSKLMHASSSRSSKSASNNSSSNEDSKTSMLTQEGSPDNSTGKKESSKGDPIETSPAAEPLLTLKLGKRFYFEDVSTGSHSKKASSSAVPLLCGKKGKSSSQNMLNPSCQVEGCGLDLSLAKDYHRKHRICDSHSKSPVVVVAGLERRFCQQCSRFHDLSEFDDKKRSCRRRLSDHNARRRKPQPEAVKLNPSALSSSPYDGRQTMGPFAFPKNTSNLAWPDMPNSKLPQTKDFMLKTPKNFSKFVTMLSDDSSGHFISKGKGTKIAVPETTVIHIDRIHITGLEDPNTLSDPNATQDVNRALSLLSTNSWGPYDTKPPSLIHSNRTTGTPQYATAQRSPFSSLEYWHTDQHQASSSACISFSGYDNSNRFQDFQLFSEPYESSFPCNQLD*