AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0423620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423620 MtrunA17_Chr3g0125261 98.127 267 5 0 1 267 1 267 0.0 545
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423620 AT1G77290.3 68.914 267 82 1 1 267 1 266 7.63e-141 397
Msa0423620 AT1G77290.1 68.914 267 82 1 1 267 1 266 7.63e-141 397
Msa0423620 AT1G77290.2 68.914 267 82 1 1 267 1 266 7.63e-141 397
Msa0423620 AT4G02520.1 24.280 243 143 4 1 237 4 211 7.00e-14 69.3

Find 86 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGCTGAGTATTGGATTT+TGG 0.152263 3_2:+73561478 Msa0423620:CDS
TCCTGTAACTGGCAAGAATT+TGG 0.171996 3_2:+73560521 Msa0423620:CDS
TTTAGCTCGTTTAAAACTTT+TGG 0.180573 3_2:+73561421 Msa0423620:CDS
ACTGAGTGAAACACCTTATT+TGG 0.190936 3_2:+73561355 Msa0423620:CDS
CTCGGTGTTTCTCTGGTATA+TGG 0.211375 3_2:-73561148 None:intergenic
TCCAAATTCTTGCCAGTTAC+AGG 0.236554 3_2:-73560522 None:intergenic
AACTCTTCGCCTGCCAAATA+AGG 0.264517 3_2:-73561368 None:intergenic
TTACTTATTCAACCATTTCT+TGG 0.265920 3_2:-73561649 None:intergenic
AGGCTTCTCTCAACTTTCTA+TGG 0.276841 3_2:-73561244 None:intergenic
TGGTATGCACCTGCTAATTC+AGG 0.285480 3_2:-73561224 None:intergenic
ATATTAGAACTTTGCTTAAT+CGG 0.286800 3_2:+73561594 Msa0423620:CDS
ATATCATCATCCGTTTGATT+TGG 0.293342 3_2:+73560434 Msa0423620:CDS
CGGATTCATCTGAAAGAATA+AGG 0.297726 3_2:-73560547 None:intergenic
CCCTGAACTGTTTCAGTACT+TGG 0.317517 3_2:-73561892 None:intergenic
TACTTGGTCCAATGAAATTC+TGG 0.327240 3_2:-73561876 None:intergenic
TTTACGTTTCCAAGTTCTTA+AGG 0.342669 3_2:+73561171 Msa0423620:CDS
CCAATGCAAGTCTCACCTTC+TGG 0.346297 3_2:-73560461 None:intergenic
TGTGTATTATTGATTGATAT+TGG 0.347592 3_2:+73561995 Msa0423620:three_prime_UTR
AAATGATGTGAGAGCCGTTT+TGG 0.353112 3_2:-73560590 None:intergenic
AGACTTGCATTGGAAGAAAA+AGG 0.354375 3_2:+73560471 Msa0423620:CDS
TGGAACAATGCCTTCTTGTA+AGG 0.374184 3_2:+73562015 Msa0423620:three_prime_UTR
TTCCATACCAATCCTGTAAC+TGG 0.385351 3_2:+73560510 Msa0423620:CDS
GAGAAGGAGCGAGGAGCATT+TGG 0.386161 3_2:+73561304 Msa0423620:CDS
GTTTGTCTCCAGAATTTCAT+TGG 0.390685 3_2:+73561868 Msa0423620:three_prime_UTR
CACCACACGCCTTAAGAACT+TGG 0.391896 3_2:-73561180 None:intergenic
TTTATTTCTCCCTTACTGCT+TGG 0.399542 3_2:+73561768 Msa0423620:three_prime_UTR
ACCAAGTACTGAAACAGTTC+AGG 0.403387 3_2:+73561891 Msa0423620:three_prime_UTR
CTCTCATGGTTGCCAAGAAA+TGG 0.406293 3_2:+73561637 Msa0423620:three_prime_UTR
ATCCAATACTCAGCAATGTT+TGG 0.413409 3_2:-73561473 None:intergenic
TGTTATGTGTGACACTAGTT+TGG 0.426185 3_2:+73562047 Msa0423620:three_prime_UTR
ACGTAAACTCGGTGTTTCTC+TGG 0.433917 3_2:-73561155 None:intergenic
GGCCAAACATTGCTGAGTAT+TGG 0.435036 3_2:+73561471 Msa0423620:CDS
CCACCTAGGAAGTTGCTGAT+AGG 0.448934 3_2:-73560393 None:intergenic
AGAATTGCTGTGGTCTCCGC+AGG 0.450946 3_2:+73561038 Msa0423620:CDS
CTGAGGATATCAGTAGCAAT+AGG 0.470164 3_2:+73561063 Msa0423620:CDS
TTTGCTTAATCGGTACTAGT+AGG 0.481196 3_2:+73561604 Msa0423620:exon
ATTCTTGCCAGTTACAGGAT+TGG 0.485946 3_2:-73560517 None:intergenic
ACTAACATCACCAAGCAGTA+AGG 0.489289 3_2:-73561778 None:intergenic
GTTGTTGTTTATTGACAACT+TGG 0.490653 3_2:+73560282 Msa0423620:five_prime_UTR
TTCTGGCTGTCCAAATCAAA+CGG 0.493106 3_2:-73560444 None:intergenic
ACAAAACATTGTTCAAAACT+TGG 0.494072 3_2:+73561561 Msa0423620:CDS
TGCCAGTTACAGGATTGGTA+TGG 0.497143 3_2:-73560512 None:intergenic
GCAATCACCTTTCTATAACT+AGG 0.510277 3_2:-73561512 None:intergenic
GGTTAGATTGCTTGATGAAG+CGG 0.515391 3_2:+73561325 Msa0423620:CDS
ATACCTATCAGCAACTTCCT+AGG 0.523035 3_2:+73560390 Msa0423620:five_prime_UTR
GGTATGCACCTGCTAATTCA+GGG 0.526259 3_2:-73561223 None:intergenic
TCGGTGTTTCTCTGGTATAT+GGG 0.528301 3_2:-73561147 None:intergenic
ATGAGAAGGGAGGAAAATCA+AGG 0.529179 3_2:+73561831 Msa0423620:three_prime_UTR
GGATGAAGAGGATACAAGAG+TGG 0.530840 3_2:+73561102 Msa0423620:CDS
AGTGAAACACCTTATTTGGC+AGG 0.533423 3_2:+73561359 Msa0423620:CDS
TGTGGTGATCGCTCGTATGT+CGG 0.536691 3_2:+73561196 Msa0423620:CDS
TCGGAGTCCCCTGAATTAGC+AGG 0.546117 3_2:+73561215 Msa0423620:CDS
TGTGGTCTCCGCAGGGTCTG+AGG 0.546519 3_2:+73561046 Msa0423620:CDS
CCAGAAGGTGAGACTTGCAT+TGG 0.548807 3_2:+73560461 Msa0423620:CDS
GCAGAGGCCTAGTTATAGAA+AGG 0.552401 3_2:+73561505 Msa0423620:CDS
ATAGGAGTGAAGTGATTGAA+TGG 0.553982 3_2:+73561081 Msa0423620:CDS
AATCAAGGTATGCATGTAAG+TGG 0.555188 3_2:+73561846 Msa0423620:three_prime_UTR
TTCCAAGTTCTTAAGGCGTG+TGG 0.557777 3_2:+73561178 Msa0423620:CDS
GTTTCTCTGGTATATGGGAG+AGG 0.560412 3_2:-73561142 None:intergenic
CACTTATCCTAGAATGAGAA+GGG 0.565199 3_2:+73561818 Msa0423620:three_prime_UTR
CTAACATCACCAAGCAGTAA+GGG 0.567559 3_2:-73561777 None:intergenic
CATTGTCAAAAGAAAAGAAG+TGG 0.576035 3_2:+73561929 Msa0423620:three_prime_UTR
GGGTGAAATAAAGTAGCCAT+TGG 0.581067 3_2:-73562119 None:intergenic
AGAATGAGTACATAACTATG+AGG 0.584947 3_2:+73561450 Msa0423620:CDS
CGCTCCTTCTCAAAACATGA+GGG 0.586987 3_2:-73561292 None:intergenic
CCAAGTACTGAAACAGTTCA+GGG 0.589412 3_2:+73561892 Msa0423620:three_prime_UTR
GCACTTATCCTAGAATGAGA+AGG 0.590458 3_2:+73561817 Msa0423620:three_prime_UTR
TCGCTCCTTCTCAAAACATG+AGG 0.593199 3_2:-73561293 None:intergenic
AAGCAGCAATCCTTACAAGA+AGG 0.594753 3_2:-73562025 None:intergenic
GGCAGGCGAAGAGTTTAGCA+TGG 0.595558 3_2:+73561376 Msa0423620:CDS
GTTTGATTTGGACAGCCAGA+AGG 0.595603 3_2:+73560446 Msa0423620:CDS
TAGGTACATATATACTCTCA+TGG 0.604593 3_2:+73561623 Msa0423620:three_prime_UTR
AAAACAGGAAGGCTTCCACT+CGG 0.612067 3_2:-73560567 None:intergenic
TTTCTCTGGTATATGGGAGA+GGG 0.623180 3_2:-73561141 None:intergenic
GATGAAGAGGATACAAGAGT+GGG 0.626925 3_2:+73561103 Msa0423620:CDS
AGAACTTGGAAACGTAAACT+CGG 0.634065 3_2:-73561166 None:intergenic
GTACATAGAAAGAATTGCTG+TGG 0.635061 3_2:+73561028 Msa0423620:CDS
TAGATCAAGAACGTAACCAA+TGG 0.635563 3_2:+73562103 Msa0423620:three_prime_UTR
AAGTGATTGAATGGATGAAG+AGG 0.651845 3_2:+73561090 Msa0423620:CDS
TTCTTTCAGATGAATCCGAG+TGG 0.664252 3_2:+73560552 Msa0423620:CDS
GAATTGCTGTGGTCTCCGCA+GGG 0.673553 3_2:+73561039 Msa0423620:CDS
AAAATAAAAGATAGGGCACA+CGG 0.678796 3_2:-73561720 None:intergenic
GTATGCACCTGCTAATTCAG+GGG 0.678849 3_2:-73561222 None:intergenic
TTATCCTAGAATGAGAAGGG+AGG 0.687206 3_2:+73561821 Msa0423620:three_prime_UTR
CCTATCAGCAACTTCCTAGG+TGG 0.692964 3_2:+73560393 Msa0423620:five_prime_UTR
ACTGATATCCTCAGACCCTG+CGG 0.699635 3_2:-73561054 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATCTTTAAACTACTATA+TGG - chr3_2:73560749-73560768 None:intergenic 15.0%
!! TTCAACAAAAATAAAAGATA+GGG - chr3_2:73561730-73561749 None:intergenic 15.0%
!! TTTCAACAAAAATAAAAGAT+AGG - chr3_2:73561731-73561750 None:intergenic 15.0%
!!! TTTATTTTTGTTGAAATAGA+AGG + chr3_2:73561735-73561754 Msa0423620:three_prime_UTR 15.0%
!!! TTATTTTTGTTGAAATAGAA+GGG + chr3_2:73561736-73561755 Msa0423620:three_prime_UTR 15.0%
!! TTTATCTTTAAACTACTATA+TGG - chr3_2:73560749-73560768 None:intergenic 15.0%
!! TTCAACAAAAATAAAAGATA+GGG - chr3_2:73561730-73561749 None:intergenic 15.0%
!! TTTCAACAAAAATAAAAGAT+AGG - chr3_2:73561731-73561750 None:intergenic 15.0%
!!! TTTATTTTTGTTGAAATAGA+AGG + chr3_2:73561735-73561754 Msa0423620:three_prime_UTR 15.0%
!!! TTATTTTTGTTGAAATAGAA+GGG + chr3_2:73561736-73561755 Msa0423620:three_prime_UTR 15.0%
!! GAAGATTTATACATCATTAT+AGG + chr3_2:73560719-73560738 Msa0423620:intron 20.0%
!! ATTCAACTTTACTTTCTAAA+CGG + chr3_2:73560819-73560838 Msa0423620:intron 20.0%
!! ATATTAGAACTTTGCTTAAT+CGG + chr3_2:73561594-73561613 Msa0423620:CDS 20.0%
!!! TATTTTTGTTGAAATAGAAG+GGG + chr3_2:73561737-73561756 Msa0423620:three_prime_UTR 20.0%
!!! TGTGTATTATTGATTGATAT+TGG + chr3_2:73561995-73562014 Msa0423620:three_prime_UTR 20.0%
!! GAAGATTTATACATCATTAT+AGG + chr3_2:73560719-73560738 Msa0423620:intron 20.0%
!! ATTCAACTTTACTTTCTAAA+CGG + chr3_2:73560819-73560838 Msa0423620:intron 20.0%
!! ATATTAGAACTTTGCTTAAT+CGG + chr3_2:73561594-73561613 Msa0423620:CDS 20.0%
!!! TATTTTTGTTGAAATAGAAG+GGG + chr3_2:73561737-73561756 Msa0423620:three_prime_UTR 20.0%
!!! TGTGTATTATTGATTGATAT+TGG + chr3_2:73561995-73562014 Msa0423620:three_prime_UTR 20.0%
! AATAATGAAGAAGGTTTGAA+GGG - chr3_2:73560649-73560668 None:intergenic 25.0%
! ATGCTACATTGAATATAAAG+TGG + chr3_2:73560874-73560893 Msa0423620:intron 25.0%
! TTCAACATTTCATTCTTAGT+GGG - chr3_2:73560943-73560962 None:intergenic 25.0%
!!! AGTTTTAAACGAGCTAAAAT+CGG - chr3_2:73561419-73561438 None:intergenic 25.0%
!!! TTTAGCTCGTTTAAAACTTT+TGG + chr3_2:73561421-73561440 Msa0423620:CDS 25.0%
! ACAAAACATTGTTCAAAACT+TGG + chr3_2:73561561-73561580 Msa0423620:CDS 25.0%
! TTACTTATTCAACCATTTCT+TGG - chr3_2:73561652-73561671 None:intergenic 25.0%
!! ATTTTTGTTGAAATAGAAGG+GGG + chr3_2:73561738-73561757 Msa0423620:three_prime_UTR 25.0%
!! TTCTTTTCTTTTGACAATGA+AGG - chr3_2:73561928-73561947 None:intergenic 25.0%
! AATAATGAAGAAGGTTTGAA+GGG - chr3_2:73560649-73560668 None:intergenic 25.0%
! ATGCTACATTGAATATAAAG+TGG + chr3_2:73560874-73560893 Msa0423620:intron 25.0%
! TTCAACATTTCATTCTTAGT+GGG - chr3_2:73560943-73560962 None:intergenic 25.0%
!!! AGTTTTAAACGAGCTAAAAT+CGG - chr3_2:73561419-73561438 None:intergenic 25.0%
!!! TTTAGCTCGTTTAAAACTTT+TGG + chr3_2:73561421-73561440 Msa0423620:CDS 25.0%
! ACAAAACATTGTTCAAAACT+TGG + chr3_2:73561561-73561580 Msa0423620:CDS 25.0%
! TTACTTATTCAACCATTTCT+TGG - chr3_2:73561652-73561671 None:intergenic 25.0%
!! ATTTTTGTTGAAATAGAAGG+GGG + chr3_2:73561738-73561757 Msa0423620:three_prime_UTR 25.0%
!! TTCTTTTCTTTTGACAATGA+AGG - chr3_2:73561928-73561947 None:intergenic 25.0%
!! GTTGTTGTTTATTGACAACT+TGG + chr3_2:73560282-73560301 Msa0423620:five_prime_UTR 30.0%
CAAAAATCACTCAGAACATT+GGG - chr3_2:73560331-73560350 None:intergenic 30.0%
ATATCATCATCCGTTTGATT+TGG + chr3_2:73560434-73560453 Msa0423620:CDS 30.0%
GAATAATGAAGAAGGTTTGA+AGG - chr3_2:73560650-73560669 None:intergenic 30.0%
TGATGACAGAATAATGAAGA+AGG - chr3_2:73560658-73560677 None:intergenic 30.0%
AAAGTTGAATATGCAGAAAG+CGG - chr3_2:73560809-73560828 None:intergenic 30.0%
CAATGTAGCATATACACAAT+AGG - chr3_2:73560865-73560884 None:intergenic 30.0%
! AAGCTTTTATCGAGAATTGA+TGG - chr3_2:73560903-73560922 None:intergenic 30.0%
CTTCAACATTTCATTCTTAG+TGG - chr3_2:73560944-73560963 None:intergenic 30.0%
TTTACGTTTCCAAGTTCTTA+AGG + chr3_2:73561171-73561190 Msa0423620:CDS 30.0%
! CAACTTTTCTTCAGTTTGAT+AGG - chr3_2:73561267-73561286 None:intergenic 30.0%
AGAATGAGTACATAACTATG+AGG + chr3_2:73561450-73561469 Msa0423620:CDS 30.0%
! GTGATTGCTAAACATTTTGA+TGG + chr3_2:73561527-73561546 Msa0423620:CDS 30.0%
TAGGTACATATATACTCTCA+TGG + chr3_2:73561623-73561642 Msa0423620:three_prime_UTR 30.0%
AAAATAAAAGATAGGGCACA+CGG - chr3_2:73561723-73561742 None:intergenic 30.0%
CATTGTCAAAAGAAAAGAAG+TGG + chr3_2:73561929-73561948 Msa0423620:three_prime_UTR 30.0%
!! GTTGTTGTTTATTGACAACT+TGG + chr3_2:73560282-73560301 Msa0423620:five_prime_UTR 30.0%
CAAAAATCACTCAGAACATT+GGG - chr3_2:73560331-73560350 None:intergenic 30.0%
ATATCATCATCCGTTTGATT+TGG + chr3_2:73560434-73560453 Msa0423620:CDS 30.0%
GAATAATGAAGAAGGTTTGA+AGG - chr3_2:73560650-73560669 None:intergenic 30.0%
TGATGACAGAATAATGAAGA+AGG - chr3_2:73560658-73560677 None:intergenic 30.0%
AAAGTTGAATATGCAGAAAG+CGG - chr3_2:73560809-73560828 None:intergenic 30.0%
CAATGTAGCATATACACAAT+AGG - chr3_2:73560865-73560884 None:intergenic 30.0%
! AAGCTTTTATCGAGAATTGA+TGG - chr3_2:73560903-73560922 None:intergenic 30.0%
CTTCAACATTTCATTCTTAG+TGG - chr3_2:73560944-73560963 None:intergenic 30.0%
TTTACGTTTCCAAGTTCTTA+AGG + chr3_2:73561171-73561190 Msa0423620:CDS 30.0%
! CAACTTTTCTTCAGTTTGAT+AGG - chr3_2:73561267-73561286 None:intergenic 30.0%
AGAATGAGTACATAACTATG+AGG + chr3_2:73561450-73561469 Msa0423620:CDS 30.0%
! GTGATTGCTAAACATTTTGA+TGG + chr3_2:73561527-73561546 Msa0423620:CDS 30.0%
TAGGTACATATATACTCTCA+TGG + chr3_2:73561623-73561642 Msa0423620:three_prime_UTR 30.0%
AAAATAAAAGATAGGGCACA+CGG - chr3_2:73561723-73561742 None:intergenic 30.0%
CATTGTCAAAAGAAAAGAAG+TGG + chr3_2:73561929-73561948 Msa0423620:three_prime_UTR 30.0%
CCAAAAATCACTCAGAACAT+TGG - chr3_2:73560332-73560351 None:intergenic 35.0%
!! CCAATGTTCTGAGTGATTTT+TGG + chr3_2:73560329-73560348 Msa0423620:five_prime_UTR 35.0%
!!! TTCTGAGTGATTTTTGGTGT+TGG + chr3_2:73560335-73560354 Msa0423620:five_prime_UTR 35.0%
! GCATTTTATTATCACCACCT+AGG - chr3_2:73560410-73560429 None:intergenic 35.0%
AGACTTGCATTGGAAGAAAA+AGG + chr3_2:73560471-73560490 Msa0423620:CDS 35.0%
CGGATTCATCTGAAAGAATA+AGG - chr3_2:73560550-73560569 None:intergenic 35.0%
CTTCATTATTCTGTCATCAC+TGG + chr3_2:73560659-73560678 Msa0423620:intron 35.0%
ACAAATGTCTTACAGTGCTA+TGG + chr3_2:73560996-73561015 Msa0423620:intron 35.0%
! TACAGTGCTATGGATTTTTC+AGG + chr3_2:73561006-73561025 Msa0423620:intron 35.0%
GTACATAGAAAGAATTGCTG+TGG + chr3_2:73561028-73561047 Msa0423620:CDS 35.0%
ATAGGAGTGAAGTGATTGAA+TGG + chr3_2:73561081-73561100 Msa0423620:CDS 35.0%
AAGTGATTGAATGGATGAAG+AGG + chr3_2:73561090-73561109 Msa0423620:CDS 35.0%
TGGGAGAGGGAAAAATATTT+AGG - chr3_2:73561131-73561150 None:intergenic 35.0%
AGAACTTGGAAACGTAAACT+CGG - chr3_2:73561169-73561188 None:intergenic 35.0%
ACTGAGTGAAACACCTTATT+TGG + chr3_2:73561355-73561374 Msa0423620:CDS 35.0%
ATCCAATACTCAGCAATGTT+TGG - chr3_2:73561476-73561495 None:intergenic 35.0%
!!! CATTGCTGAGTATTGGATTT+TGG + chr3_2:73561478-73561497 Msa0423620:CDS 35.0%
GCAATCACCTTTCTATAACT+AGG - chr3_2:73561515-73561534 None:intergenic 35.0%
!! TTGCTAAACATTTTGATGGC+TGG + chr3_2:73561531-73561550 Msa0423620:CDS 35.0%
TTTGCTTAATCGGTACTAGT+AGG + chr3_2:73561604-73561623 Msa0423620:exon 35.0%
! TTTATTTCTCCCTTACTGCT+TGG + chr3_2:73561768-73561787 Msa0423620:three_prime_UTR 35.0%
CACTTATCCTAGAATGAGAA+GGG + chr3_2:73561818-73561837 Msa0423620:three_prime_UTR 35.0%
AATCAAGGTATGCATGTAAG+TGG + chr3_2:73561846-73561865 Msa0423620:three_prime_UTR 35.0%
GTTTGTCTCCAGAATTTCAT+TGG + chr3_2:73561868-73561887 Msa0423620:three_prime_UTR 35.0%
TACTTGGTCCAATGAAATTC+TGG - chr3_2:73561879-73561898 None:intergenic 35.0%
! CTTTTGACAATGAAGGAAGA+TGG - chr3_2:73561921-73561940 None:intergenic 35.0%
TGTTATGTGTGACACTAGTT+TGG + chr3_2:73562047-73562066 Msa0423620:three_prime_UTR 35.0%
TAGATCAAGAACGTAACCAA+TGG + chr3_2:73562103-73562122 Msa0423620:three_prime_UTR 35.0%
CCAAAAATCACTCAGAACAT+TGG - chr3_2:73560332-73560351 None:intergenic 35.0%
!! CCAATGTTCTGAGTGATTTT+TGG + chr3_2:73560329-73560348 Msa0423620:five_prime_UTR 35.0%
!!! TTCTGAGTGATTTTTGGTGT+TGG + chr3_2:73560335-73560354 Msa0423620:five_prime_UTR 35.0%
! GCATTTTATTATCACCACCT+AGG - chr3_2:73560410-73560429 None:intergenic 35.0%
AGACTTGCATTGGAAGAAAA+AGG + chr3_2:73560471-73560490 Msa0423620:CDS 35.0%
CGGATTCATCTGAAAGAATA+AGG - chr3_2:73560550-73560569 None:intergenic 35.0%
CTTCATTATTCTGTCATCAC+TGG + chr3_2:73560659-73560678 Msa0423620:intron 35.0%
ACAAATGTCTTACAGTGCTA+TGG + chr3_2:73560996-73561015 Msa0423620:intron 35.0%
! TACAGTGCTATGGATTTTTC+AGG + chr3_2:73561006-73561025 Msa0423620:intron 35.0%
GTACATAGAAAGAATTGCTG+TGG + chr3_2:73561028-73561047 Msa0423620:CDS 35.0%
ATAGGAGTGAAGTGATTGAA+TGG + chr3_2:73561081-73561100 Msa0423620:CDS 35.0%
AAGTGATTGAATGGATGAAG+AGG + chr3_2:73561090-73561109 Msa0423620:CDS 35.0%
TGGGAGAGGGAAAAATATTT+AGG - chr3_2:73561131-73561150 None:intergenic 35.0%
AGAACTTGGAAACGTAAACT+CGG - chr3_2:73561169-73561188 None:intergenic 35.0%
ACTGAGTGAAACACCTTATT+TGG + chr3_2:73561355-73561374 Msa0423620:CDS 35.0%
ATCCAATACTCAGCAATGTT+TGG - chr3_2:73561476-73561495 None:intergenic 35.0%
!!! CATTGCTGAGTATTGGATTT+TGG + chr3_2:73561478-73561497 Msa0423620:CDS 35.0%
GCAATCACCTTTCTATAACT+AGG - chr3_2:73561515-73561534 None:intergenic 35.0%
!! TTGCTAAACATTTTGATGGC+TGG + chr3_2:73561531-73561550 Msa0423620:CDS 35.0%
TTTGCTTAATCGGTACTAGT+AGG + chr3_2:73561604-73561623 Msa0423620:exon 35.0%
! TTTATTTCTCCCTTACTGCT+TGG + chr3_2:73561768-73561787 Msa0423620:three_prime_UTR 35.0%
CACTTATCCTAGAATGAGAA+GGG + chr3_2:73561818-73561837 Msa0423620:three_prime_UTR 35.0%
AATCAAGGTATGCATGTAAG+TGG + chr3_2:73561846-73561865 Msa0423620:three_prime_UTR 35.0%
GTTTGTCTCCAGAATTTCAT+TGG + chr3_2:73561868-73561887 Msa0423620:three_prime_UTR 35.0%
TACTTGGTCCAATGAAATTC+TGG - chr3_2:73561879-73561898 None:intergenic 35.0%
! CTTTTGACAATGAAGGAAGA+TGG - chr3_2:73561921-73561940 None:intergenic 35.0%
TGTTATGTGTGACACTAGTT+TGG + chr3_2:73562047-73562066 Msa0423620:three_prime_UTR 35.0%
TAGATCAAGAACGTAACCAA+TGG + chr3_2:73562103-73562122 Msa0423620:three_prime_UTR 35.0%
ATACCTATCAGCAACTTCCT+AGG + chr3_2:73560390-73560409 Msa0423620:five_prime_UTR 40.0%
! TTCTGGCTGTCCAAATCAAA+CGG - chr3_2:73560447-73560466 None:intergenic 40.0%
TTCCATACCAATCCTGTAAC+TGG + chr3_2:73560510-73560529 Msa0423620:CDS 40.0%
ATTCTTGCCAGTTACAGGAT+TGG - chr3_2:73560520-73560539 None:intergenic 40.0%
TCCTGTAACTGGCAAGAATT+TGG + chr3_2:73560521-73560540 Msa0423620:CDS 40.0%
TCCAAATTCTTGCCAGTTAC+AGG - chr3_2:73560525-73560544 None:intergenic 40.0%
TTCTTTCAGATGAATCCGAG+TGG + chr3_2:73560552-73560571 Msa0423620:CDS 40.0%
! AGCCTTCCTGTTTTCCAAAA+CGG + chr3_2:73560576-73560595 Msa0423620:CDS 40.0%
! AAATGATGTGAGAGCCGTTT+TGG - chr3_2:73560593-73560612 None:intergenic 40.0%
AATAGGCTGCTTCAGTTCAT+GGG - chr3_2:73560848-73560867 None:intergenic 40.0%
CTGAGGATATCAGTAGCAAT+AGG + chr3_2:73561063-73561082 Msa0423620:CDS 40.0%
GATGAAGAGGATACAAGAGT+GGG + chr3_2:73561103-73561122 Msa0423620:CDS 40.0%
TTTCTCTGGTATATGGGAGA+GGG - chr3_2:73561144-73561163 None:intergenic 40.0%
! TCGGTGTTTCTCTGGTATAT+GGG - chr3_2:73561150-73561169 None:intergenic 40.0%
AGGCTTCTCTCAACTTTCTA+TGG - chr3_2:73561247-73561266 None:intergenic 40.0%
!!! AAGACCCTCATGTTTTGAGA+AGG + chr3_2:73561288-73561307 Msa0423620:CDS 40.0%
GGTTAGATTGCTTGATGAAG+CGG + chr3_2:73561325-73561344 Msa0423620:CDS 40.0%
! AGTGAAACACCTTATTTGGC+AGG + chr3_2:73561359-73561378 Msa0423620:CDS 40.0%
!!! ATTGGATTTTGGTTCAGCAG+AGG + chr3_2:73561489-73561508 Msa0423620:CDS 40.0%
CTAACATCACCAAGCAGTAA+GGG - chr3_2:73561780-73561799 None:intergenic 40.0%
ACTAACATCACCAAGCAGTA+AGG - chr3_2:73561781-73561800 None:intergenic 40.0%
GCACTTATCCTAGAATGAGA+AGG + chr3_2:73561817-73561836 Msa0423620:three_prime_UTR 40.0%
TTATCCTAGAATGAGAAGGG+AGG + chr3_2:73561821-73561840 Msa0423620:three_prime_UTR 40.0%
! TTTTCCTCCCTTCTCATTCT+AGG - chr3_2:73561828-73561847 None:intergenic 40.0%
ATGAGAAGGGAGGAAAATCA+AGG + chr3_2:73561831-73561850 Msa0423620:three_prime_UTR 40.0%
!! ACCAAGTACTGAAACAGTTC+AGG + chr3_2:73561891-73561910 Msa0423620:three_prime_UTR 40.0%
!! CCAAGTACTGAAACAGTTCA+GGG + chr3_2:73561892-73561911 Msa0423620:three_prime_UTR 40.0%
TGGAACAATGCCTTCTTGTA+AGG + chr3_2:73562015-73562034 Msa0423620:three_prime_UTR 40.0%
AAGCAGCAATCCTTACAAGA+AGG - chr3_2:73562028-73562047 None:intergenic 40.0%
ATACCTATCAGCAACTTCCT+AGG + chr3_2:73560390-73560409 Msa0423620:five_prime_UTR 40.0%
! TTCTGGCTGTCCAAATCAAA+CGG - chr3_2:73560447-73560466 None:intergenic 40.0%
TTCCATACCAATCCTGTAAC+TGG + chr3_2:73560510-73560529 Msa0423620:CDS 40.0%
ATTCTTGCCAGTTACAGGAT+TGG - chr3_2:73560520-73560539 None:intergenic 40.0%
TCCTGTAACTGGCAAGAATT+TGG + chr3_2:73560521-73560540 Msa0423620:CDS 40.0%
TCCAAATTCTTGCCAGTTAC+AGG - chr3_2:73560525-73560544 None:intergenic 40.0%
TTCTTTCAGATGAATCCGAG+TGG + chr3_2:73560552-73560571 Msa0423620:CDS 40.0%
! AGCCTTCCTGTTTTCCAAAA+CGG + chr3_2:73560576-73560595 Msa0423620:CDS 40.0%
! AAATGATGTGAGAGCCGTTT+TGG - chr3_2:73560593-73560612 None:intergenic 40.0%
AATAGGCTGCTTCAGTTCAT+GGG - chr3_2:73560848-73560867 None:intergenic 40.0%
CTGAGGATATCAGTAGCAAT+AGG + chr3_2:73561063-73561082 Msa0423620:CDS 40.0%
GATGAAGAGGATACAAGAGT+GGG + chr3_2:73561103-73561122 Msa0423620:CDS 40.0%
TTTCTCTGGTATATGGGAGA+GGG - chr3_2:73561144-73561163 None:intergenic 40.0%
! TCGGTGTTTCTCTGGTATAT+GGG - chr3_2:73561150-73561169 None:intergenic 40.0%
AGGCTTCTCTCAACTTTCTA+TGG - chr3_2:73561247-73561266 None:intergenic 40.0%
!!! AAGACCCTCATGTTTTGAGA+AGG + chr3_2:73561288-73561307 Msa0423620:CDS 40.0%
GGTTAGATTGCTTGATGAAG+CGG + chr3_2:73561325-73561344 Msa0423620:CDS 40.0%
! AGTGAAACACCTTATTTGGC+AGG + chr3_2:73561359-73561378 Msa0423620:CDS 40.0%
!!! ATTGGATTTTGGTTCAGCAG+AGG + chr3_2:73561489-73561508 Msa0423620:CDS 40.0%
CTAACATCACCAAGCAGTAA+GGG - chr3_2:73561780-73561799 None:intergenic 40.0%
ACTAACATCACCAAGCAGTA+AGG - chr3_2:73561781-73561800 None:intergenic 40.0%
GCACTTATCCTAGAATGAGA+AGG + chr3_2:73561817-73561836 Msa0423620:three_prime_UTR 40.0%
TTATCCTAGAATGAGAAGGG+AGG + chr3_2:73561821-73561840 Msa0423620:three_prime_UTR 40.0%
! TTTTCCTCCCTTCTCATTCT+AGG - chr3_2:73561828-73561847 None:intergenic 40.0%
ATGAGAAGGGAGGAAAATCA+AGG + chr3_2:73561831-73561850 Msa0423620:three_prime_UTR 40.0%
!! ACCAAGTACTGAAACAGTTC+AGG + chr3_2:73561891-73561910 Msa0423620:three_prime_UTR 40.0%
!! CCAAGTACTGAAACAGTTCA+GGG + chr3_2:73561892-73561911 Msa0423620:three_prime_UTR 40.0%
TGGAACAATGCCTTCTTGTA+AGG + chr3_2:73562015-73562034 Msa0423620:three_prime_UTR 40.0%
AAGCAGCAATCCTTACAAGA+AGG - chr3_2:73562028-73562047 None:intergenic 40.0%
!! GTTTGATTTGGACAGCCAGA+AGG + chr3_2:73560446-73560465 Msa0423620:CDS 45.0%
! TGCCAGTTACAGGATTGGTA+TGG - chr3_2:73560515-73560534 None:intergenic 45.0%
AAAACAGGAAGGCTTCCACT+CGG - chr3_2:73560570-73560589 None:intergenic 45.0%
!!! AGCCGTTTTGGAAAACAGGA+AGG - chr3_2:73560581-73560600 None:intergenic 45.0%
!!! TGAGAGCCGTTTTGGAAAAC+AGG - chr3_2:73560585-73560604 None:intergenic 45.0%
CAATAGGCTGCTTCAGTTCA+TGG - chr3_2:73560849-73560868 None:intergenic 45.0%
GGATGAAGAGGATACAAGAG+TGG + chr3_2:73561102-73561121 Msa0423620:CDS 45.0%
GTTTCTCTGGTATATGGGAG+AGG - chr3_2:73561145-73561164 None:intergenic 45.0%
!! CTCGGTGTTTCTCTGGTATA+TGG - chr3_2:73561151-73561170 None:intergenic 45.0%
!! ACGTAAACTCGGTGTTTCTC+TGG - chr3_2:73561158-73561177 None:intergenic 45.0%
TTCCAAGTTCTTAAGGCGTG+TGG + chr3_2:73561178-73561197 Msa0423620:CDS 45.0%
GTATGCACCTGCTAATTCAG+GGG - chr3_2:73561225-73561244 None:intergenic 45.0%
GGTATGCACCTGCTAATTCA+GGG - chr3_2:73561226-73561245 None:intergenic 45.0%
TGGTATGCACCTGCTAATTC+AGG - chr3_2:73561227-73561246 None:intergenic 45.0%
CGCTCCTTCTCAAAACATGA+GGG - chr3_2:73561295-73561314 None:intergenic 45.0%
TCGCTCCTTCTCAAAACATG+AGG - chr3_2:73561296-73561315 None:intergenic 45.0%
!!! TCATGTTTTGAGAAGGAGCG+AGG + chr3_2:73561295-73561314 Msa0423620:CDS 45.0%
AACTCTTCGCCTGCCAAATA+AGG - chr3_2:73561371-73561390 None:intergenic 45.0%
GGCCAAACATTGCTGAGTAT+TGG + chr3_2:73561471-73561490 Msa0423620:CDS 45.0%
GCAGAGGCCTAGTTATAGAA+AGG + chr3_2:73561505-73561524 Msa0423620:CDS 45.0%
CTCTCATGGTTGCCAAGAAA+TGG + chr3_2:73561637-73561656 Msa0423620:three_prime_UTR 45.0%
CCCTGAACTGTTTCAGTACT+TGG - chr3_2:73561895-73561914 None:intergenic 45.0%
!! GTTTGATTTGGACAGCCAGA+AGG + chr3_2:73560446-73560465 Msa0423620:CDS 45.0%
! TGCCAGTTACAGGATTGGTA+TGG - chr3_2:73560515-73560534 None:intergenic 45.0%
AAAACAGGAAGGCTTCCACT+CGG - chr3_2:73560570-73560589 None:intergenic 45.0%
!!! AGCCGTTTTGGAAAACAGGA+AGG - chr3_2:73560581-73560600 None:intergenic 45.0%
!!! TGAGAGCCGTTTTGGAAAAC+AGG - chr3_2:73560585-73560604 None:intergenic 45.0%
CAATAGGCTGCTTCAGTTCA+TGG - chr3_2:73560849-73560868 None:intergenic 45.0%
GGATGAAGAGGATACAAGAG+TGG + chr3_2:73561102-73561121 Msa0423620:CDS 45.0%
GTTTCTCTGGTATATGGGAG+AGG - chr3_2:73561145-73561164 None:intergenic 45.0%
!! CTCGGTGTTTCTCTGGTATA+TGG - chr3_2:73561151-73561170 None:intergenic 45.0%
!! ACGTAAACTCGGTGTTTCTC+TGG - chr3_2:73561158-73561177 None:intergenic 45.0%
TTCCAAGTTCTTAAGGCGTG+TGG + chr3_2:73561178-73561197 Msa0423620:CDS 45.0%
GTATGCACCTGCTAATTCAG+GGG - chr3_2:73561225-73561244 None:intergenic 45.0%
GGTATGCACCTGCTAATTCA+GGG - chr3_2:73561226-73561245 None:intergenic 45.0%
TGGTATGCACCTGCTAATTC+AGG - chr3_2:73561227-73561246 None:intergenic 45.0%
CGCTCCTTCTCAAAACATGA+GGG - chr3_2:73561295-73561314 None:intergenic 45.0%
TCGCTCCTTCTCAAAACATG+AGG - chr3_2:73561296-73561315 None:intergenic 45.0%
!!! TCATGTTTTGAGAAGGAGCG+AGG + chr3_2:73561295-73561314 Msa0423620:CDS 45.0%
AACTCTTCGCCTGCCAAATA+AGG - chr3_2:73561371-73561390 None:intergenic 45.0%
GGCCAAACATTGCTGAGTAT+TGG + chr3_2:73561471-73561490 Msa0423620:CDS 45.0%
GCAGAGGCCTAGTTATAGAA+AGG + chr3_2:73561505-73561524 Msa0423620:CDS 45.0%
CTCTCATGGTTGCCAAGAAA+TGG + chr3_2:73561637-73561656 Msa0423620:three_prime_UTR 45.0%
CCCTGAACTGTTTCAGTACT+TGG - chr3_2:73561895-73561914 None:intergenic 45.0%
CCACCTAGGAAGTTGCTGAT+AGG - chr3_2:73560396-73560415 None:intergenic 50.0%
CCTATCAGCAACTTCCTAGG+TGG + chr3_2:73560393-73560412 Msa0423620:five_prime_UTR 50.0%
CCAATGCAAGTCTCACCTTC+TGG - chr3_2:73560464-73560483 None:intergenic 50.0%
CCAGAAGGTGAGACTTGCAT+TGG + chr3_2:73560461-73560480 Msa0423620:CDS 50.0%
ACTGATATCCTCAGACCCTG+CGG - chr3_2:73561057-73561076 None:intergenic 50.0%
CACCACACGCCTTAAGAACT+TGG - chr3_2:73561183-73561202 None:intergenic 50.0%
TGTGGTGATCGCTCGTATGT+CGG + chr3_2:73561196-73561215 Msa0423620:CDS 50.0%
CCACCTAGGAAGTTGCTGAT+AGG - chr3_2:73560396-73560415 None:intergenic 50.0%
CCTATCAGCAACTTCCTAGG+TGG + chr3_2:73560393-73560412 Msa0423620:five_prime_UTR 50.0%
CCAATGCAAGTCTCACCTTC+TGG - chr3_2:73560464-73560483 None:intergenic 50.0%
CCAGAAGGTGAGACTTGCAT+TGG + chr3_2:73560461-73560480 Msa0423620:CDS 50.0%
ACTGATATCCTCAGACCCTG+CGG - chr3_2:73561057-73561076 None:intergenic 50.0%
CACCACACGCCTTAAGAACT+TGG - chr3_2:73561183-73561202 None:intergenic 50.0%
TGTGGTGATCGCTCGTATGT+CGG + chr3_2:73561196-73561215 Msa0423620:CDS 50.0%
AGAATTGCTGTGGTCTCCGC+AGG + chr3_2:73561038-73561057 Msa0423620:CDS 55.0%
GAATTGCTGTGGTCTCCGCA+GGG + chr3_2:73561039-73561058 Msa0423620:CDS 55.0%
TCGGAGTCCCCTGAATTAGC+AGG + chr3_2:73561215-73561234 Msa0423620:CDS 55.0%
!! GAGAAGGAGCGAGGAGCATT+TGG + chr3_2:73561304-73561323 Msa0423620:CDS 55.0%
GGCAGGCGAAGAGTTTAGCA+TGG + chr3_2:73561376-73561395 Msa0423620:CDS 55.0%
AGAATTGCTGTGGTCTCCGC+AGG + chr3_2:73561038-73561057 Msa0423620:CDS 55.0%
GAATTGCTGTGGTCTCCGCA+GGG + chr3_2:73561039-73561058 Msa0423620:CDS 55.0%
TCGGAGTCCCCTGAATTAGC+AGG + chr3_2:73561215-73561234 Msa0423620:CDS 55.0%
!! GAGAAGGAGCGAGGAGCATT+TGG + chr3_2:73561304-73561323 Msa0423620:CDS 55.0%
GGCAGGCGAAGAGTTTAGCA+TGG + chr3_2:73561376-73561395 Msa0423620:CDS 55.0%
TGTGGTCTCCGCAGGGTCTG+AGG + chr3_2:73561046-73561065 Msa0423620:CDS 65.0%
TGTGGTCTCCGCAGGGTCTG+AGG + chr3_2:73561046-73561065 Msa0423620:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 73560278 73562136 73560278 ID=Msa0423620;Name=Msa0423620
chr3_2 mRNA 73560278 73562136 73560278 ID=Msa0423620-mRNA-1;Parent=Msa0423620;Name=Msa0423620-mRNA-1;_AED=0.01;_eAED=0.01;_QI=148|1|1|1|1|1|2|514|267
chr3_2 exon 73560278 73560634 73560278 ID=Msa0423620-mRNA-1:exon:18322;Parent=Msa0423620-mRNA-1
chr3_2 exon 73561028 73562136 73561028 ID=Msa0423620-mRNA-1:exon:18323;Parent=Msa0423620-mRNA-1
chr3_2 five_prime_UTR 73560278 73560425 73560278 ID=Msa0423620-mRNA-1:five_prime_utr;Parent=Msa0423620-mRNA-1
chr3_2 CDS 73560426 73560634 73560426 ID=Msa0423620-mRNA-1:cds;Parent=Msa0423620-mRNA-1
chr3_2 CDS 73561028 73561622 73561028 ID=Msa0423620-mRNA-1:cds;Parent=Msa0423620-mRNA-1
chr3_2 three_prime_UTR 73561623 73562136 73561623 ID=Msa0423620-mRNA-1:three_prime_utr;Parent=Msa0423620-mRNA-1
Gene Sequence

>Msa0423620

ATGCAGCTATATCATCATCCGTTTGATTTGGACAGCCAGAAGGTGAGACTTGCATTGGAAGAAAAAGGCATTGATTATACATCTTTCCATACCAATCCTGTAACTGGCAAGAATTTGGATTCCTTATTCTTTCAGATGAATCCGAGTGGAAGCCTTCCTGTTTTCCAAAACGGCTCTCACATCATTTACAAGACTATTGATATAATTCAGTACATAGAAAGAATTGCTGTGGTCTCCGCAGGGTCTGAGGATATCAGTAGCAATAGGAGTGAAGTGATTGAATGGATGAAGAGGATACAAGAGTGGGATCCTAAATATTTTTCCCTCTCCCATATACCAGAGAAACACCGAGTTTACGTTTCCAAGTTCTTAAGGCGTGTGGTGATCGCTCGTATGTCGGAGTCCCCTGAATTAGCAGGTGCATACCATAGAAAGTTGAGAGAAGCCTATCAAACTGAAGAAAAGTTGAAAGACCCTCATGTTTTGAGAAGGAGCGAGGAGCATTTGGTTAGATTGCTTGATGAAGCGGAAAGACAACTGAGTGAAACACCTTATTTGGCAGGCGAAGAGTTTAGCATGGCTGATGTGATGCTCATTCCGATTTTAGCTCGTTTAAAACTTTTGGATTTAGAGAATGAGTACATAACTATGAGGCCAAACATTGCTGAGTATTGGATTTTGGTTCAGCAGAGGCCTAGTTATAGAAAGGTGATTGCTAAACATTTTGATGGCTGGAGAAAGCACAAAACATTGTTCAAAACTTGGTGCTTTGTTCATATTAGAACTTTGCTTAATCGGTACTAG
Protein sequence

>Msa0423620

MQLYHHPFDLDSQKVRLALEEKGIDYTSFHTNPVTGKNLDSLFFQMNPSGSLPVFQNGSHIIYKTIDIIQYIERIAVVSAGSEDISSNRSEVIEWMKRIQEWDPKYFSLSHIPEKHRVYVSKFLRRVVIARMSESPELAGAYHRKLREAYQTEEKLKDPHVLRRSEEHLVRLLDEAERQLSETPYLAGEEFSMADVMLIPILARLKLLDLENEYITMRPNIAEYWILVQQRPSYRKVIAKHFDGWRKHKTLFKTWCFVHIRTLLNRY*