AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0624670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0624670 MtrunA17_Chr3g0125261 98.127 267 5 0 1 267 1 267 0.0 545
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0624670 AT1G77290.3 68.914 267 82 1 1 267 1 266 7.63e-141 397
Msa0624670 AT1G77290.1 68.914 267 82 1 1 267 1 266 7.63e-141 397
Msa0624670 AT1G77290.2 68.914 267 82 1 1 267 1 266 7.63e-141 397
Msa0624670 AT4G02520.1 24.280 243 143 4 1 237 4 211 7.00e-14 69.3

Find 88 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTCTTCTTTCTTCTTC+TGG 0.032764 4_3:+3209612 Msa0624670:five_prime_UTR
CATTGCTGAGTATTGGATTT+TGG 0.152263 4_3:+3211606 Msa0624670:CDS
TCCTGTAACTGGCAAGAATT+TGG 0.171996 4_3:+3210648 Msa0624670:CDS
TTTAGCTCGTTTAAAACTTT+TGG 0.180573 4_3:+3211549 Msa0624670:CDS
ACTGAGTGAAACACCTTATT+TGG 0.190936 4_3:+3211483 Msa0624670:CDS
CTCGGTGTTTCTCTGGTATA+TGG 0.211375 4_3:-3211276 None:intergenic
TCCAAATTCTTGCCAGTTAC+AGG 0.236554 4_3:-3210649 None:intergenic
AACTCTTCGCCTGCCAAATA+AGG 0.264517 4_3:-3211496 None:intergenic
TTACTTATTCAACCATTTCT+TGG 0.265920 4_3:-3211777 None:intergenic
TCTTTCTTCTTTCTTCTTCT+GGG 0.273205 4_3:+3209613 Msa0624670:five_prime_UTR
AGGCTTCTCTCAACTTTCTA+TGG 0.276841 4_3:-3211372 None:intergenic
TGGTATGCACCTGCTAATTC+AGG 0.285480 4_3:-3211352 None:intergenic
ATATTAGAACTTTGCTTAAT+CGG 0.286800 4_3:+3211722 Msa0624670:CDS
ATATCATCATCCGTTTGATT+TGG 0.293342 4_3:+3210561 Msa0624670:CDS
CGGATTCATCTGAAAGAATA+AGG 0.297726 4_3:-3210674 None:intergenic
AAGTGGAGAGAGTGAGAAAA+AGG 0.302361 4_3:-3209562 None:intergenic
CCCTGAACTGTTTCAGTACT+TGG 0.317517 4_3:-3212021 None:intergenic
TACTTGGTCCAATGAAATTC+TGG 0.327240 4_3:-3212005 None:intergenic
TTTACGTTTCCAAGTTCTTA+AGG 0.342669 4_3:+3211299 Msa0624670:CDS
CCAATGCAAGTCTCACCTTC+TGG 0.346297 4_3:-3210588 None:intergenic
TGTGTATTATTGATTGATAT+TGG 0.347592 4_3:+3212124 Msa0624670:three_prime_UTR
AAATGATGTGAGAGCCGTTT+TGG 0.353112 4_3:-3210717 None:intergenic
AGACTTGCATTGGAAGAAAA+AGG 0.354375 4_3:+3210598 Msa0624670:CDS
GTTATGTGTGACACTAGTTT+TGG 0.361960 4_3:+3212177 Msa0624670:three_prime_UTR
TGGAACAATGCCTTCTTGTA+AGG 0.374184 4_3:+3212144 Msa0624670:three_prime_UTR
TTCCATACCAATCCTGTAAC+TGG 0.385351 4_3:+3210637 Msa0624670:CDS
GAGAAGGAGCGAGGAGCATT+TGG 0.386161 4_3:+3211432 Msa0624670:CDS
GTTTGTCTCCAGAATTTCAT+TGG 0.390685 4_3:+3211997 Msa0624670:three_prime_UTR
CACCACACGCCTTAAGAACT+TGG 0.391896 4_3:-3211308 None:intergenic
TTTATTTCTCCCTTACTGCT+TGG 0.399542 4_3:+3211897 Msa0624670:three_prime_UTR
ACCAAGTACTGAAACAGTTC+AGG 0.403387 4_3:+3212020 Msa0624670:three_prime_UTR
CTCTCATGGTTGCCAAGAAA+TGG 0.406293 4_3:+3211765 Msa0624670:three_prime_UTR
ATCCAATACTCAGCAATGTT+TGG 0.413409 4_3:-3211601 None:intergenic
ACGTAAACTCGGTGTTTCTC+TGG 0.433917 4_3:-3211283 None:intergenic
GGCCAAACATTGCTGAGTAT+TGG 0.435036 4_3:+3211599 Msa0624670:CDS
CCACCTAGGAAGTTGCTGAT+AGG 0.448934 4_3:-3210520 None:intergenic
AGAATTGCTGTGGTCTCCGC+AGG 0.450946 4_3:+3211166 Msa0624670:CDS
CTGAGGATATCAGTAGCAAT+AGG 0.470164 4_3:+3211191 Msa0624670:CDS
TTTGCTTAATCGGTACTAGT+AGG 0.481148 4_3:+3211732 Msa0624670:exon
AAGAAAGAAGAAAAGAAGAA+AGG 0.482172 4_3:-3209598 None:intergenic
ATTCTTGCCAGTTACAGGAT+TGG 0.485946 4_3:-3210644 None:intergenic
ACTAACATCACCAAGCAGTA+AGG 0.489289 4_3:-3211907 None:intergenic
TTCTGGCTGTCCAAATCAAA+CGG 0.493106 4_3:-3210571 None:intergenic
ACAAAACATTGTTCAAAACT+TGG 0.494072 4_3:+3211689 Msa0624670:CDS
TGCCAGTTACAGGATTGGTA+TGG 0.497143 4_3:-3210639 None:intergenic
GCAATCACCTTTCTATAACT+AGG 0.510277 4_3:-3211640 None:intergenic
GGTTAGATTGCTTGATGAAG+CGG 0.515391 4_3:+3211453 Msa0624670:CDS
ATACCTATCAGCAACTTCCT+AGG 0.523035 4_3:+3210517 Msa0624670:five_prime_UTR
GGTATGCACCTGCTAATTCA+GGG 0.526259 4_3:-3211351 None:intergenic
TCGGTGTTTCTCTGGTATAT+GGG 0.528301 4_3:-3211275 None:intergenic
ATGAGAAGGGAGGAAAATCA+AGG 0.529179 4_3:+3211960 Msa0624670:three_prime_UTR
GGATGAAGAGGATACAAGAG+TGG 0.530840 4_3:+3211230 Msa0624670:CDS
AGTGAAACACCTTATTTGGC+AGG 0.533423 4_3:+3211487 Msa0624670:CDS
TGTGGTGATCGCTCGTATGT+CGG 0.536691 4_3:+3211324 Msa0624670:CDS
TCGGAGTCCCCTGAATTAGC+AGG 0.546117 4_3:+3211343 Msa0624670:CDS
TGTGGTCTCCGCAGGGTCTG+AGG 0.546519 4_3:+3211174 Msa0624670:CDS
CCAGAAGGTGAGACTTGCAT+TGG 0.548807 4_3:+3210588 Msa0624670:CDS
GCAGAGGCCTAGTTATAGAA+AGG 0.552401 4_3:+3211633 Msa0624670:CDS
ATAGGAGTGAAGTGATTGAA+TGG 0.553982 4_3:+3211209 Msa0624670:CDS
AATCAAGGTATGCATGTAAG+TGG 0.555188 4_3:+3211975 Msa0624670:three_prime_UTR
TTCCAAGTTCTTAAGGCGTG+TGG 0.557777 4_3:+3211306 Msa0624670:CDS
GTTTCTCTGGTATATGGGAG+AGG 0.560412 4_3:-3211270 None:intergenic
CACTTATCCTAGAATGAGAA+GGG 0.565199 4_3:+3211947 Msa0624670:three_prime_UTR
CTAACATCACCAAGCAGTAA+GGG 0.567559 4_3:-3211906 None:intergenic
CATTGTCAAAAGAAAAGAAG+TGG 0.576035 4_3:+3212058 Msa0624670:three_prime_UTR
GGGTGAAATAAAGTAGCCAT+TGG 0.581067 4_3:-3212249 None:intergenic
AGAATGAGTACATAACTATG+AGG 0.584947 4_3:+3211578 Msa0624670:CDS
CGCTCCTTCTCAAAACATGA+GGG 0.586987 4_3:-3211420 None:intergenic
CCAAGTACTGAAACAGTTCA+GGG 0.589412 4_3:+3212021 Msa0624670:three_prime_UTR
GCACTTATCCTAGAATGAGA+AGG 0.590458 4_3:+3211946 Msa0624670:three_prime_UTR
TCGCTCCTTCTCAAAACATG+AGG 0.593199 4_3:-3211421 None:intergenic
AAGCAGCAATCCTTACAAGA+AGG 0.594753 4_3:-3212154 None:intergenic
GGCAGGCGAAGAGTTTAGCA+TGG 0.595558 4_3:+3211504 Msa0624670:CDS
GTTTGATTTGGACAGCCAGA+AGG 0.595603 4_3:+3210573 Msa0624670:CDS
AAAACAGGAAGGCTTCCACT+CGG 0.612067 4_3:-3210694 None:intergenic
TTTCTCTGGTATATGGGAGA+GGG 0.623180 4_3:-3211269 None:intergenic
TAGGTGCATATATACTCTCA+TGG 0.625805 4_3:+3211751 Msa0624670:three_prime_UTR
GATGAAGAGGATACAAGAGT+GGG 0.626925 4_3:+3211231 Msa0624670:CDS
AGAACTTGGAAACGTAAACT+CGG 0.634065 4_3:-3211294 None:intergenic
GTACATAGAAAGAATTGCTG+TGG 0.635061 4_3:+3211156 Msa0624670:CDS
TAGATCAAGAACGTAACCAA+TGG 0.635563 4_3:+3212233 Msa0624670:three_prime_UTR
AAGTGATTGAATGGATGAAG+AGG 0.651845 4_3:+3211218 Msa0624670:CDS
TTCTTTCAGATGAATCCGAG+TGG 0.664252 4_3:+3210679 Msa0624670:CDS
GAATTGCTGTGGTCTCCGCA+GGG 0.673553 4_3:+3211167 Msa0624670:CDS
GTATGCACCTGCTAATTCAG+GGG 0.678849 4_3:-3211350 None:intergenic
TTATCCTAGAATGAGAAGGG+AGG 0.687206 4_3:+3211950 Msa0624670:three_prime_UTR
CCTATCAGCAACTTCCTAGG+TGG 0.692964 4_3:+3210520 Msa0624670:five_prime_UTR
ACTGATATCCTCAGACCCTG+CGG 0.699635 4_3:-3211182 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTGTTGAAATAAA+AGG + chr4_3:3211864-3211883 Msa0624670:three_prime_UTR 10.0%
!!! TTATTTTTGTTGAAATAAAA+GGG + chr4_3:3211865-3211884 Msa0624670:three_prime_UTR 10.0%
!! ATATTTGATATTTCAATATG+TGG + chr4_3:3210183-3210202 Msa0624670:intron 15.0%
!! TTTATCTTTAAACTACTATA+TGG - chr4_3:3210876-3210895 None:intergenic 15.0%
!! TCAACAAAAATAAAAAGATA+GGG - chr4_3:3211858-3211877 None:intergenic 15.0%
!! TTCAACAAAAATAAAAAGAT+AGG - chr4_3:3211859-3211878 None:intergenic 15.0%
!!! TATTTTTGTTGAAATAAAAG+GGG + chr4_3:3211866-3211885 Msa0624670:three_prime_UTR 15.0%
!! AAAAATCAATAAATTCAGTG+AGG - chr4_3:3209799-3209818 None:intergenic 20.0%
!!! AGATAATTTTATCCGATAAA+GGG - chr4_3:3209856-3209875 None:intergenic 20.0%
!!! AAGATAATTTTATCCGATAA+AGG - chr4_3:3209857-3209876 None:intergenic 20.0%
!! TTCAATTTACAAAAACAAGA+CGG - chr4_3:3209918-3209937 None:intergenic 20.0%
!!! ATTGAAATTACTTTTTCGAT+TGG + chr4_3:3209932-3209951 Msa0624670:intron 20.0%
!! TAATCAAAGATGTTGAATAT+TGG + chr4_3:3209965-3209984 Msa0624670:intron 20.0%
!! GAAGATTTATACATCATTAT+AGG + chr4_3:3210846-3210865 Msa0624670:intron 20.0%
!! ATTCAACTTTACTTTCTAAA+CGG + chr4_3:3210946-3210965 Msa0624670:intron 20.0%
!! ATATTAGAACTTTGCTTAAT+CGG + chr4_3:3211722-3211741 Msa0624670:CDS 20.0%
!!! ATTTTTGTTGAAATAAAAGG+GGG + chr4_3:3211867-3211886 Msa0624670:three_prime_UTR 20.0%
!!! TGTGTATTATTGATTGATAT+TGG + chr4_3:3212124-3212143 Msa0624670:three_prime_UTR 20.0%
!! AGAAAAGAAGAAAGGTTTTA+GGG - chr4_3:3209593-3209612 None:intergenic 25.0%
!! AAGAAAAGAAGAAAGGTTTT+AGG - chr4_3:3209594-3209613 None:intergenic 25.0%
! AAGAAAGAAGAAAAGAAGAA+AGG - chr4_3:3209601-3209620 None:intergenic 25.0%
! TACTTTAACAAACCCTTTAT+CGG + chr4_3:3209841-3209860 Msa0624670:intron 25.0%
! ACTTCAGTACTCTTTATATT+AGG + chr4_3:3210036-3210055 Msa0624670:intron 25.0%
! AGCACTAAAATGAAATGAAA+GGG - chr4_3:3210099-3210118 None:intergenic 25.0%
! TAGCACTAAAATGAAATGAA+AGG - chr4_3:3210100-3210119 None:intergenic 25.0%
! TGATATCACTTCTTACTTAT+AGG + chr4_3:3210252-3210271 Msa0624670:intron 25.0%
! AATAATGAAGAAGGTTTGAA+GGG - chr4_3:3210776-3210795 None:intergenic 25.0%
! ATGCTACATTGAATATAAAG+TGG + chr4_3:3211002-3211021 Msa0624670:intron 25.0%
! TTCAACATTTCATTCTTAGT+GGG - chr4_3:3211071-3211090 None:intergenic 25.0%
!!! AGTTTTAAACGAGCTAAAAT+CGG - chr4_3:3211547-3211566 None:intergenic 25.0%
!!! TTTAGCTCGTTTAAAACTTT+TGG + chr4_3:3211549-3211568 Msa0624670:CDS 25.0%
! ACAAAACATTGTTCAAAACT+TGG + chr4_3:3211689-3211708 Msa0624670:CDS 25.0%
! TTACTTATTCAACCATTTCT+TGG - chr4_3:3211780-3211799 None:intergenic 25.0%
!! TTCTTTTCTTTTGACAATGA+AGG - chr4_3:3212057-3212076 None:intergenic 25.0%
TTCTTTCTTCTTTCTTCTTC+TGG + chr4_3:3209612-3209631 Msa0624670:five_prime_UTR 30.0%
TCTTTCTTCTTTCTTCTTCT+GGG + chr4_3:3209613-3209632 Msa0624670:five_prime_UTR 30.0%
! TGCTTTCACTTGTGTTTTAT+CGG + chr4_3:3209725-3209744 Msa0624670:intron 30.0%
GCATGTCAAATTTCTGTTAT+TGG + chr4_3:3210142-3210161 Msa0624670:intron 30.0%
TTCACATTATTCTGTCGTAA+AGG - chr4_3:3210355-3210374 None:intergenic 30.0%
!! GTTGTTGTTTATTGACAACT+TGG + chr4_3:3210409-3210428 Msa0624670:intron 30.0%
CAAAAATCACTCAGAACATT+GGG - chr4_3:3210458-3210477 None:intergenic 30.0%
ATATCATCATCCGTTTGATT+TGG + chr4_3:3210561-3210580 Msa0624670:CDS 30.0%
GAATAATGAAGAAGGTTTGA+AGG - chr4_3:3210777-3210796 None:intergenic 30.0%
TGATGACAGAATAATGAAGA+AGG - chr4_3:3210785-3210804 None:intergenic 30.0%
AAAGTTGAATATGCAGAAAG+CGG - chr4_3:3210936-3210955 None:intergenic 30.0%
CAATGTAGCATATACACAAT+AGG - chr4_3:3210993-3211012 None:intergenic 30.0%
! AAGCTTTTATCGAGAATTGA+TGG - chr4_3:3211031-3211050 None:intergenic 30.0%
CTTCAACATTTCATTCTTAG+TGG - chr4_3:3211072-3211091 None:intergenic 30.0%
TTTACGTTTCCAAGTTCTTA+AGG + chr4_3:3211299-3211318 Msa0624670:CDS 30.0%
! CAACTTTTCTTCAGTTTGAT+AGG - chr4_3:3211395-3211414 None:intergenic 30.0%
AGAATGAGTACATAACTATG+AGG + chr4_3:3211578-3211597 Msa0624670:CDS 30.0%
! GTGATTGCTAAACATTTTGA+TGG + chr4_3:3211655-3211674 Msa0624670:CDS 30.0%
AAATAAAAAGATAGGGCACA+CGG - chr4_3:3211851-3211870 None:intergenic 30.0%
CATTGTCAAAAGAAAAGAAG+TGG + chr4_3:3212058-3212077 Msa0624670:three_prime_UTR 30.0%
!!! AAGGTTTTAGGGATTGAAAG+TGG - chr4_3:3209582-3209601 None:intergenic 35.0%
! TCACTTGTGTTTTATCGGAT+TGG + chr4_3:3209730-3209749 Msa0624670:intron 35.0%
ACAATGTAAGAAAGATTGGC+AGG - chr4_3:3210308-3210327 None:intergenic 35.0%
CGAGACAATGTAAGAAAGAT+TGG - chr4_3:3210312-3210331 None:intergenic 35.0%
CCAAAAATCACTCAGAACAT+TGG - chr4_3:3210459-3210478 None:intergenic 35.0%
!! CCAATGTTCTGAGTGATTTT+TGG + chr4_3:3210456-3210475 Msa0624670:five_prime_UTR 35.0%
!!! TTCTGAGTGATTTTTGGTGT+TGG + chr4_3:3210462-3210481 Msa0624670:five_prime_UTR 35.0%
! GCATTTTATTATCACCACCT+AGG - chr4_3:3210537-3210556 None:intergenic 35.0%
AGACTTGCATTGGAAGAAAA+AGG + chr4_3:3210598-3210617 Msa0624670:CDS 35.0%
CGGATTCATCTGAAAGAATA+AGG - chr4_3:3210677-3210696 None:intergenic 35.0%
CTTCATTATTCTGTCATCAC+TGG + chr4_3:3210786-3210805 Msa0624670:intron 35.0%
ACAAATGTCTTACAGTGCTA+TGG + chr4_3:3211124-3211143 Msa0624670:intron 35.0%
! TACAGTGCTATGGATTTTTC+AGG + chr4_3:3211134-3211153 Msa0624670:intron 35.0%
GTACATAGAAAGAATTGCTG+TGG + chr4_3:3211156-3211175 Msa0624670:CDS 35.0%
ATAGGAGTGAAGTGATTGAA+TGG + chr4_3:3211209-3211228 Msa0624670:CDS 35.0%
AAGTGATTGAATGGATGAAG+AGG + chr4_3:3211218-3211237 Msa0624670:CDS 35.0%
TGGGAGAGGGAAAAATATTT+AGG - chr4_3:3211259-3211278 None:intergenic 35.0%
AGAACTTGGAAACGTAAACT+CGG - chr4_3:3211297-3211316 None:intergenic 35.0%
ACTGAGTGAAACACCTTATT+TGG + chr4_3:3211483-3211502 Msa0624670:CDS 35.0%
ATCCAATACTCAGCAATGTT+TGG - chr4_3:3211604-3211623 None:intergenic 35.0%
!!! CATTGCTGAGTATTGGATTT+TGG + chr4_3:3211606-3211625 Msa0624670:CDS 35.0%
GCAATCACCTTTCTATAACT+AGG - chr4_3:3211643-3211662 None:intergenic 35.0%
!! TTGCTAAACATTTTGATGGC+TGG + chr4_3:3211659-3211678 Msa0624670:CDS 35.0%
TTTGCTTAATCGGTACTAGT+AGG + chr4_3:3211732-3211751 Msa0624670:exon 35.0%
TAGGTGCATATATACTCTCA+TGG + chr4_3:3211751-3211770 Msa0624670:three_prime_UTR 35.0%
! TTTATTTCTCCCTTACTGCT+TGG + chr4_3:3211897-3211916 Msa0624670:three_prime_UTR 35.0%
CACTTATCCTAGAATGAGAA+GGG + chr4_3:3211947-3211966 Msa0624670:three_prime_UTR 35.0%
AATCAAGGTATGCATGTAAG+TGG + chr4_3:3211975-3211994 Msa0624670:three_prime_UTR 35.0%
GTTTGTCTCCAGAATTTCAT+TGG + chr4_3:3211997-3212016 Msa0624670:three_prime_UTR 35.0%
TACTTGGTCCAATGAAATTC+TGG - chr4_3:3212008-3212027 None:intergenic 35.0%
! CTTTTGACAATGAAGGAAGA+TGG - chr4_3:3212050-3212069 None:intergenic 35.0%
!! GTTATGTGTGACACTAGTTT+TGG + chr4_3:3212177-3212196 Msa0624670:three_prime_UTR 35.0%
TAGATCAAGAACGTAACCAA+TGG + chr4_3:3212233-3212252 Msa0624670:three_prime_UTR 35.0%
AAGTGGAGAGAGTGAGAAAA+AGG - chr4_3:3209565-3209584 None:intergenic 40.0%
! TATAGGCTTCTGTCACGTTT+TGG + chr4_3:3210269-3210288 Msa0624670:intron 40.0%
ATACCTATCAGCAACTTCCT+AGG + chr4_3:3210517-3210536 Msa0624670:five_prime_UTR 40.0%
! TTCTGGCTGTCCAAATCAAA+CGG - chr4_3:3210574-3210593 None:intergenic 40.0%
TTCCATACCAATCCTGTAAC+TGG + chr4_3:3210637-3210656 Msa0624670:CDS 40.0%
ATTCTTGCCAGTTACAGGAT+TGG - chr4_3:3210647-3210666 None:intergenic 40.0%
TCCTGTAACTGGCAAGAATT+TGG + chr4_3:3210648-3210667 Msa0624670:CDS 40.0%
TCCAAATTCTTGCCAGTTAC+AGG - chr4_3:3210652-3210671 None:intergenic 40.0%
TTCTTTCAGATGAATCCGAG+TGG + chr4_3:3210679-3210698 Msa0624670:CDS 40.0%
! AGCCTTCCTGTTTTCCAAAA+CGG + chr4_3:3210703-3210722 Msa0624670:CDS 40.0%
! AAATGATGTGAGAGCCGTTT+TGG - chr4_3:3210720-3210739 None:intergenic 40.0%
! ATAGGCTGCTTCAAGTTCAT+GGG - chr4_3:3210975-3210994 None:intergenic 40.0%
! AATAGGCTGCTTCAAGTTCA+TGG - chr4_3:3210976-3210995 None:intergenic 40.0%
CTGAGGATATCAGTAGCAAT+AGG + chr4_3:3211191-3211210 Msa0624670:CDS 40.0%
GATGAAGAGGATACAAGAGT+GGG + chr4_3:3211231-3211250 Msa0624670:CDS 40.0%
TTTCTCTGGTATATGGGAGA+GGG - chr4_3:3211272-3211291 None:intergenic 40.0%
! TCGGTGTTTCTCTGGTATAT+GGG - chr4_3:3211278-3211297 None:intergenic 40.0%
AGGCTTCTCTCAACTTTCTA+TGG - chr4_3:3211375-3211394 None:intergenic 40.0%
!!! AAGACCCTCATGTTTTGAGA+AGG + chr4_3:3211416-3211435 Msa0624670:CDS 40.0%
GGTTAGATTGCTTGATGAAG+CGG + chr4_3:3211453-3211472 Msa0624670:CDS 40.0%
! AGTGAAACACCTTATTTGGC+AGG + chr4_3:3211487-3211506 Msa0624670:CDS 40.0%
!!! ATTGGATTTTGGTTCAGCAG+AGG + chr4_3:3211617-3211636 Msa0624670:CDS 40.0%
CTAACATCACCAAGCAGTAA+GGG - chr4_3:3211909-3211928 None:intergenic 40.0%
ACTAACATCACCAAGCAGTA+AGG - chr4_3:3211910-3211929 None:intergenic 40.0%
GCACTTATCCTAGAATGAGA+AGG + chr4_3:3211946-3211965 Msa0624670:three_prime_UTR 40.0%
TTATCCTAGAATGAGAAGGG+AGG + chr4_3:3211950-3211969 Msa0624670:three_prime_UTR 40.0%
! TTTTCCTCCCTTCTCATTCT+AGG - chr4_3:3211957-3211976 None:intergenic 40.0%
ATGAGAAGGGAGGAAAATCA+AGG + chr4_3:3211960-3211979 Msa0624670:three_prime_UTR 40.0%
!! ACCAAGTACTGAAACAGTTC+AGG + chr4_3:3212020-3212039 Msa0624670:three_prime_UTR 40.0%
!! CCAAGTACTGAAACAGTTCA+GGG + chr4_3:3212021-3212040 Msa0624670:three_prime_UTR 40.0%
TGGAACAATGCCTTCTTGTA+AGG + chr4_3:3212144-3212163 Msa0624670:three_prime_UTR 40.0%
AAGCAGCAATCCTTACAAGA+AGG - chr4_3:3212157-3212176 None:intergenic 40.0%
GGGTGAAATAAAGTAGCCAT+TGG - chr4_3:3212252-3212271 None:intergenic 40.0%
!! GTTTGATTTGGACAGCCAGA+AGG + chr4_3:3210573-3210592 Msa0624670:CDS 45.0%
! TGCCAGTTACAGGATTGGTA+TGG - chr4_3:3210642-3210661 None:intergenic 45.0%
AAAACAGGAAGGCTTCCACT+CGG - chr4_3:3210697-3210716 None:intergenic 45.0%
!!! AGCCGTTTTGGAAAACAGGA+AGG - chr4_3:3210708-3210727 None:intergenic 45.0%
!!! TGAGAGCCGTTTTGGAAAAC+AGG - chr4_3:3210712-3210731 None:intergenic 45.0%
GGATGAAGAGGATACAAGAG+TGG + chr4_3:3211230-3211249 Msa0624670:CDS 45.0%
GTTTCTCTGGTATATGGGAG+AGG - chr4_3:3211273-3211292 None:intergenic 45.0%
!! CTCGGTGTTTCTCTGGTATA+TGG - chr4_3:3211279-3211298 None:intergenic 45.0%
!! ACGTAAACTCGGTGTTTCTC+TGG - chr4_3:3211286-3211305 None:intergenic 45.0%
TTCCAAGTTCTTAAGGCGTG+TGG + chr4_3:3211306-3211325 Msa0624670:CDS 45.0%
GTATGCACCTGCTAATTCAG+GGG - chr4_3:3211353-3211372 None:intergenic 45.0%
GGTATGCACCTGCTAATTCA+GGG - chr4_3:3211354-3211373 None:intergenic 45.0%
TGGTATGCACCTGCTAATTC+AGG - chr4_3:3211355-3211374 None:intergenic 45.0%
CGCTCCTTCTCAAAACATGA+GGG - chr4_3:3211423-3211442 None:intergenic 45.0%
TCGCTCCTTCTCAAAACATG+AGG - chr4_3:3211424-3211443 None:intergenic 45.0%
!!! TCATGTTTTGAGAAGGAGCG+AGG + chr4_3:3211423-3211442 Msa0624670:CDS 45.0%
AACTCTTCGCCTGCCAAATA+AGG - chr4_3:3211499-3211518 None:intergenic 45.0%
GGCCAAACATTGCTGAGTAT+TGG + chr4_3:3211599-3211618 Msa0624670:CDS 45.0%
GCAGAGGCCTAGTTATAGAA+AGG + chr4_3:3211633-3211652 Msa0624670:CDS 45.0%
CTCTCATGGTTGCCAAGAAA+TGG + chr4_3:3211765-3211784 Msa0624670:three_prime_UTR 45.0%
CCCTGAACTGTTTCAGTACT+TGG - chr4_3:3212024-3212043 None:intergenic 45.0%
!! AAAATTGATATATTAATAAA+TGG - chr4_3:3210226-3210245 None:intergenic 5.0%
CCACCTAGGAAGTTGCTGAT+AGG - chr4_3:3210523-3210542 None:intergenic 50.0%
CCTATCAGCAACTTCCTAGG+TGG + chr4_3:3210520-3210539 Msa0624670:five_prime_UTR 50.0%
CCAATGCAAGTCTCACCTTC+TGG - chr4_3:3210591-3210610 None:intergenic 50.0%
CCAGAAGGTGAGACTTGCAT+TGG + chr4_3:3210588-3210607 Msa0624670:CDS 50.0%
ACTGATATCCTCAGACCCTG+CGG - chr4_3:3211185-3211204 None:intergenic 50.0%
CACCACACGCCTTAAGAACT+TGG - chr4_3:3211311-3211330 None:intergenic 50.0%
TGTGGTGATCGCTCGTATGT+CGG + chr4_3:3211324-3211343 Msa0624670:CDS 50.0%
AGAATTGCTGTGGTCTCCGC+AGG + chr4_3:3211166-3211185 Msa0624670:CDS 55.0%
GAATTGCTGTGGTCTCCGCA+GGG + chr4_3:3211167-3211186 Msa0624670:CDS 55.0%
TCGGAGTCCCCTGAATTAGC+AGG + chr4_3:3211343-3211362 Msa0624670:CDS 55.0%
!! GAGAAGGAGCGAGGAGCATT+TGG + chr4_3:3211432-3211451 Msa0624670:CDS 55.0%
GGCAGGCGAAGAGTTTAGCA+TGG + chr4_3:3211504-3211523 Msa0624670:CDS 55.0%
TGTGGTCTCCGCAGGGTCTG+AGG + chr4_3:3211174-3211193 Msa0624670:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 3209519 3212275 3209519 ID=Msa0624670;Name=Msa0624670
chr4_3 mRNA 3209519 3212275 3209519 ID=Msa0624670-mRNA-1;Parent=Msa0624670;Name=Msa0624670-mRNA-1;_AED=0.17;_eAED=0.17;_QI=216|1|1|1|0.5|0.33|3|525|267
chr4_3 exon 3209519 3209634 3209519 ID=Msa0624670-mRNA-1:exon:734;Parent=Msa0624670-mRNA-1
chr4_3 exon 3210453 3210761 3210453 ID=Msa0624670-mRNA-1:exon:735;Parent=Msa0624670-mRNA-1
chr4_3 exon 3211156 3212275 3211156 ID=Msa0624670-mRNA-1:exon:736;Parent=Msa0624670-mRNA-1
chr4_3 five_prime_UTR 3209519 3209634 3209519 ID=Msa0624670-mRNA-1:five_prime_utr;Parent=Msa0624670-mRNA-1
chr4_3 five_prime_UTR 3210453 3210552 3210453 ID=Msa0624670-mRNA-1:five_prime_utr;Parent=Msa0624670-mRNA-1
chr4_3 CDS 3210553 3210761 3210553 ID=Msa0624670-mRNA-1:cds;Parent=Msa0624670-mRNA-1
chr4_3 CDS 3211156 3211750 3211156 ID=Msa0624670-mRNA-1:cds;Parent=Msa0624670-mRNA-1
chr4_3 three_prime_UTR 3211751 3212275 3211751 ID=Msa0624670-mRNA-1:three_prime_utr;Parent=Msa0624670-mRNA-1
Gene Sequence

>Msa0624670

ATGCAGCTATATCATCATCCGTTTGATTTGGACAGCCAGAAGGTGAGACTTGCATTGGAAGAAAAAGGCATTGATTATACATCTTTCCATACCAATCCTGTAACTGGCAAGAATTTGGATTCCTTATTCTTTCAGATGAATCCGAGTGGAAGCCTTCCTGTTTTCCAAAACGGCTCTCACATCATTTACAAGACTATTGATATAATTCAGTACATAGAAAGAATTGCTGTGGTCTCCGCAGGGTCTGAGGATATCAGTAGCAATAGGAGTGAAGTGATTGAATGGATGAAGAGGATACAAGAGTGGGATCCTAAATATTTTTCCCTCTCCCATATACCAGAGAAACACCGAGTTTACGTTTCCAAGTTCTTAAGGCGTGTGGTGATCGCTCGTATGTCGGAGTCCCCTGAATTAGCAGGTGCATACCATAGAAAGTTGAGAGAAGCCTATCAAACTGAAGAAAAGTTGAAAGACCCTCATGTTTTGAGAAGGAGCGAGGAGCATTTGGTTAGATTGCTTGATGAAGCGGAAAGACAACTGAGTGAAACACCTTATTTGGCAGGCGAAGAGTTTAGCATGGCTGATGTGATGCTCATTCCGATTTTAGCTCGTTTAAAACTTTTGGATTTAGAGAATGAGTACATAACTATGAGGCCAAACATTGCTGAGTATTGGATTTTGGTTCAGCAGAGGCCTAGTTATAGAAAGGTGATTGCTAAACATTTTGATGGCTGGAGAAAGCACAAAACATTGTTCAAAACTTGGTGCTTTGTTCATATTAGAACTTTGCTTAATCGGTACTAG
Protein sequence

>Msa0624670

MQLYHHPFDLDSQKVRLALEEKGIDYTSFHTNPVTGKNLDSLFFQMNPSGSLPVFQNGSHIIYKTIDIIQYIERIAVVSAGSEDISSNRSEVIEWMKRIQEWDPKYFSLSHIPEKHRVYVSKFLRRVVIARMSESPELAGAYHRKLREAYQTEEKLKDPHVLRRSEEHLVRLLDEAERQLSETPYLAGEEFSMADVMLIPILARLKLLDLENEYITMRPNIAEYWILVQQRPSYRKVIAKHFDGWRKHKTLFKTWCFVHIRTLLNRY*