AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0515700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515700 MtrunA17_Chr3g0125261 98.127 267 5 0 1 267 1 267 0.0 545
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515700 AT1G77290.3 68.914 267 82 1 1 267 1 266 7.63e-141 397
Msa0515700 AT1G77290.1 68.914 267 82 1 1 267 1 266 7.63e-141 397
Msa0515700 AT1G77290.2 68.914 267 82 1 1 267 1 266 7.63e-141 397
Msa0515700 AT4G02520.1 24.280 243 143 4 1 237 4 211 7.00e-14 69.3

Find 88 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTCTTCTTTCTTCTTC+TGG 0.032764 3_4:+67023445 Msa0515700:five_prime_UTR
CATTGCTGAGTATTGGATTT+TGG 0.152263 3_4:+67025439 Msa0515700:CDS
TCCTGTAACTGGCAAGAATT+TGG 0.171996 3_4:+67024481 Msa0515700:CDS
TTTAGCTCGTTTAAAACTTT+TGG 0.180573 3_4:+67025382 Msa0515700:CDS
ACTGAGTGAAACACCTTATT+TGG 0.190936 3_4:+67025316 Msa0515700:CDS
CTCGGTGTTTCTCTGGTATA+TGG 0.211375 3_4:-67025109 None:intergenic
TCCAAATTCTTGCCAGTTAC+AGG 0.236554 3_4:-67024482 None:intergenic
AACTCTTCGCCTGCCAAATA+AGG 0.264517 3_4:-67025329 None:intergenic
TTACTTATTCAACCATTTCT+TGG 0.265920 3_4:-67025610 None:intergenic
TCTTTCTTCTTTCTTCTTCT+GGG 0.273205 3_4:+67023446 Msa0515700:five_prime_UTR
AGGCTTCTCTCAACTTTCTA+TGG 0.276841 3_4:-67025205 None:intergenic
TGGTATGCACCTGCTAATTC+AGG 0.285480 3_4:-67025185 None:intergenic
ATATTAGAACTTTGCTTAAT+CGG 0.286800 3_4:+67025555 Msa0515700:CDS
ATATCATCATCCGTTTGATT+TGG 0.293342 3_4:+67024394 Msa0515700:CDS
CGGATTCATCTGAAAGAATA+AGG 0.297726 3_4:-67024507 None:intergenic
AAGTGGAGAGAGTGAGAAAA+AGG 0.302361 3_4:-67023395 None:intergenic
CCCTGAACTGTTTCAGTACT+TGG 0.317517 3_4:-67025854 None:intergenic
TACTTGGTCCAATGAAATTC+TGG 0.327240 3_4:-67025838 None:intergenic
TTTACGTTTCCAAGTTCTTA+AGG 0.342669 3_4:+67025132 Msa0515700:CDS
CCAATGCAAGTCTCACCTTC+TGG 0.346297 3_4:-67024421 None:intergenic
TGTGTATTATTGATTGATAT+TGG 0.347592 3_4:+67025957 Msa0515700:three_prime_UTR
AAATGATGTGAGAGCCGTTT+TGG 0.353112 3_4:-67024550 None:intergenic
AGACTTGCATTGGAAGAAAA+AGG 0.354375 3_4:+67024431 Msa0515700:CDS
GTTATGTGTGACACTAGTTT+TGG 0.361960 3_4:+67026010 Msa0515700:three_prime_UTR
TGGAACAATGCCTTCTTGTA+AGG 0.374184 3_4:+67025977 Msa0515700:three_prime_UTR
TTCCATACCAATCCTGTAAC+TGG 0.385351 3_4:+67024470 Msa0515700:CDS
GAGAAGGAGCGAGGAGCATT+TGG 0.386161 3_4:+67025265 Msa0515700:CDS
GTTTGTCTCCAGAATTTCAT+TGG 0.390685 3_4:+67025830 Msa0515700:three_prime_UTR
CACCACACGCCTTAAGAACT+TGG 0.391896 3_4:-67025141 None:intergenic
TTTATTTCTCCCTTACTGCT+TGG 0.399542 3_4:+67025730 Msa0515700:three_prime_UTR
ACCAAGTACTGAAACAGTTC+AGG 0.403387 3_4:+67025853 Msa0515700:three_prime_UTR
CTCTCATGGTTGCCAAGAAA+TGG 0.406293 3_4:+67025598 Msa0515700:three_prime_UTR
ATCCAATACTCAGCAATGTT+TGG 0.413409 3_4:-67025434 None:intergenic
ACGTAAACTCGGTGTTTCTC+TGG 0.433917 3_4:-67025116 None:intergenic
GGCCAAACATTGCTGAGTAT+TGG 0.435036 3_4:+67025432 Msa0515700:CDS
CCACCTAGGAAGTTGCTGAT+AGG 0.448934 3_4:-67024353 None:intergenic
AGAATTGCTGTGGTCTCCGC+AGG 0.450946 3_4:+67024999 Msa0515700:CDS
CTGAGGATATCAGTAGCAAT+AGG 0.470164 3_4:+67025024 Msa0515700:CDS
TTTGCTTAATCGGTACTAGT+AGG 0.481148 3_4:+67025565 Msa0515700:exon
AAGAAAGAAGAAAAGAAGAA+AGG 0.482172 3_4:-67023431 None:intergenic
ATTCTTGCCAGTTACAGGAT+TGG 0.485946 3_4:-67024477 None:intergenic
ACTAACATCACCAAGCAGTA+AGG 0.489289 3_4:-67025740 None:intergenic
TTCTGGCTGTCCAAATCAAA+CGG 0.493106 3_4:-67024404 None:intergenic
ACAAAACATTGTTCAAAACT+TGG 0.494072 3_4:+67025522 Msa0515700:CDS
TGCCAGTTACAGGATTGGTA+TGG 0.497143 3_4:-67024472 None:intergenic
GCAATCACCTTTCTATAACT+AGG 0.510277 3_4:-67025473 None:intergenic
GGTTAGATTGCTTGATGAAG+CGG 0.515391 3_4:+67025286 Msa0515700:CDS
ATACCTATCAGCAACTTCCT+AGG 0.523035 3_4:+67024350 Msa0515700:five_prime_UTR
GGTATGCACCTGCTAATTCA+GGG 0.526259 3_4:-67025184 None:intergenic
TCGGTGTTTCTCTGGTATAT+GGG 0.528301 3_4:-67025108 None:intergenic
ATGAGAAGGGAGGAAAATCA+AGG 0.529179 3_4:+67025793 Msa0515700:three_prime_UTR
GGATGAAGAGGATACAAGAG+TGG 0.530840 3_4:+67025063 Msa0515700:CDS
AGTGAAACACCTTATTTGGC+AGG 0.533423 3_4:+67025320 Msa0515700:CDS
TGTGGTGATCGCTCGTATGT+CGG 0.536691 3_4:+67025157 Msa0515700:CDS
TCGGAGTCCCCTGAATTAGC+AGG 0.546117 3_4:+67025176 Msa0515700:CDS
TGTGGTCTCCGCAGGGTCTG+AGG 0.546519 3_4:+67025007 Msa0515700:CDS
CCAGAAGGTGAGACTTGCAT+TGG 0.548807 3_4:+67024421 Msa0515700:CDS
GCAGAGGCCTAGTTATAGAA+AGG 0.552401 3_4:+67025466 Msa0515700:CDS
ATAGGAGTGAAGTGATTGAA+TGG 0.553982 3_4:+67025042 Msa0515700:CDS
AATCAAGGTATGCATGTAAG+TGG 0.555188 3_4:+67025808 Msa0515700:three_prime_UTR
TTCCAAGTTCTTAAGGCGTG+TGG 0.557777 3_4:+67025139 Msa0515700:CDS
GTTTCTCTGGTATATGGGAG+AGG 0.560412 3_4:-67025103 None:intergenic
CACTTATCCTAGAATGAGAA+GGG 0.565199 3_4:+67025780 Msa0515700:three_prime_UTR
CTAACATCACCAAGCAGTAA+GGG 0.567559 3_4:-67025739 None:intergenic
CATTGTCAAAAGAAAAGAAG+TGG 0.576035 3_4:+67025891 Msa0515700:three_prime_UTR
GGGTGAAATAAAGTAGCCAT+TGG 0.581067 3_4:-67026082 None:intergenic
AGAATGAGTACATAACTATG+AGG 0.584947 3_4:+67025411 Msa0515700:CDS
CGCTCCTTCTCAAAACATGA+GGG 0.586987 3_4:-67025253 None:intergenic
CCAAGTACTGAAACAGTTCA+GGG 0.589412 3_4:+67025854 Msa0515700:three_prime_UTR
GCACTTATCCTAGAATGAGA+AGG 0.590458 3_4:+67025779 Msa0515700:three_prime_UTR
TCGCTCCTTCTCAAAACATG+AGG 0.593199 3_4:-67025254 None:intergenic
AAGCAGCAATCCTTACAAGA+AGG 0.594753 3_4:-67025987 None:intergenic
GGCAGGCGAAGAGTTTAGCA+TGG 0.595558 3_4:+67025337 Msa0515700:CDS
GTTTGATTTGGACAGCCAGA+AGG 0.595603 3_4:+67024406 Msa0515700:CDS
AAAACAGGAAGGCTTCCACT+CGG 0.612067 3_4:-67024527 None:intergenic
TTTCTCTGGTATATGGGAGA+GGG 0.623180 3_4:-67025102 None:intergenic
TAGGTGCATATATACTCTCA+TGG 0.625805 3_4:+67025584 Msa0515700:three_prime_UTR
GATGAAGAGGATACAAGAGT+GGG 0.626925 3_4:+67025064 Msa0515700:CDS
AGAACTTGGAAACGTAAACT+CGG 0.634065 3_4:-67025127 None:intergenic
GTACATAGAAAGAATTGCTG+TGG 0.635061 3_4:+67024989 Msa0515700:CDS
TAGATCAAGAACGTAACCAA+TGG 0.635563 3_4:+67026066 Msa0515700:three_prime_UTR
AAGTGATTGAATGGATGAAG+AGG 0.651845 3_4:+67025051 Msa0515700:CDS
TTCTTTCAGATGAATCCGAG+TGG 0.664252 3_4:+67024512 Msa0515700:CDS
GAATTGCTGTGGTCTCCGCA+GGG 0.673553 3_4:+67025000 Msa0515700:CDS
GTATGCACCTGCTAATTCAG+GGG 0.678849 3_4:-67025183 None:intergenic
TTATCCTAGAATGAGAAGGG+AGG 0.687206 3_4:+67025783 Msa0515700:three_prime_UTR
CCTATCAGCAACTTCCTAGG+TGG 0.692964 3_4:+67024353 Msa0515700:five_prime_UTR
ACTGATATCCTCAGACCCTG+CGG 0.699635 3_4:-67025015 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTGTTGAAATAAA+AGG + chr3_4:67025697-67025716 Msa0515700:three_prime_UTR 10.0%
!!! TTATTTTTGTTGAAATAAAA+GGG + chr3_4:67025698-67025717 Msa0515700:three_prime_UTR 10.0%
!! ATATTTGATATTTCAATATG+TGG + chr3_4:67024016-67024035 Msa0515700:intron 15.0%
!! TTTATCTTTAAACTACTATA+TGG - chr3_4:67024709-67024728 None:intergenic 15.0%
!! TCAACAAAAATAAAAAGATA+GGG - chr3_4:67025691-67025710 None:intergenic 15.0%
!! TTCAACAAAAATAAAAAGAT+AGG - chr3_4:67025692-67025711 None:intergenic 15.0%
!!! TATTTTTGTTGAAATAAAAG+GGG + chr3_4:67025699-67025718 Msa0515700:three_prime_UTR 15.0%
!! AAAAATCAATAAATTCAGTG+AGG - chr3_4:67023632-67023651 None:intergenic 20.0%
!!! AGATAATTTTATCCGATAAA+GGG - chr3_4:67023689-67023708 None:intergenic 20.0%
!!! AAGATAATTTTATCCGATAA+AGG - chr3_4:67023690-67023709 None:intergenic 20.0%
!! TTCAATTTACAAAAACAAGA+CGG - chr3_4:67023751-67023770 None:intergenic 20.0%
!!! ATTGAAATTACTTTTTCGAT+TGG + chr3_4:67023765-67023784 Msa0515700:intron 20.0%
!! TAATCAAAGATGTTGAATAT+TGG + chr3_4:67023798-67023817 Msa0515700:intron 20.0%
!! GAAGATTTATACATCATTAT+AGG + chr3_4:67024679-67024698 Msa0515700:intron 20.0%
!! ATTCAACTTTACTTTCTAAA+CGG + chr3_4:67024779-67024798 Msa0515700:intron 20.0%
!! ATATTAGAACTTTGCTTAAT+CGG + chr3_4:67025555-67025574 Msa0515700:CDS 20.0%
!!! ATTTTTGTTGAAATAAAAGG+GGG + chr3_4:67025700-67025719 Msa0515700:three_prime_UTR 20.0%
!!! TGTGTATTATTGATTGATAT+TGG + chr3_4:67025957-67025976 Msa0515700:three_prime_UTR 20.0%
!! AGAAAAGAAGAAAGGTTTTA+GGG - chr3_4:67023426-67023445 None:intergenic 25.0%
!! AAGAAAAGAAGAAAGGTTTT+AGG - chr3_4:67023427-67023446 None:intergenic 25.0%
! AAGAAAGAAGAAAAGAAGAA+AGG - chr3_4:67023434-67023453 None:intergenic 25.0%
! TACTTTAACAAACCCTTTAT+CGG + chr3_4:67023674-67023693 Msa0515700:intron 25.0%
! ACTTCAGTACTCTTTATATT+AGG + chr3_4:67023869-67023888 Msa0515700:intron 25.0%
! AGCACTAAAATGAAATGAAA+GGG - chr3_4:67023932-67023951 None:intergenic 25.0%
! TAGCACTAAAATGAAATGAA+AGG - chr3_4:67023933-67023952 None:intergenic 25.0%
! TGATATCACTTCTTACTTAT+AGG + chr3_4:67024085-67024104 Msa0515700:intron 25.0%
! AATAATGAAGAAGGTTTGAA+GGG - chr3_4:67024609-67024628 None:intergenic 25.0%
! ATGCTACATTGAATATAAAG+TGG + chr3_4:67024835-67024854 Msa0515700:intron 25.0%
! TTCAACATTTCATTCTTAGT+GGG - chr3_4:67024904-67024923 None:intergenic 25.0%
!!! AGTTTTAAACGAGCTAAAAT+CGG - chr3_4:67025380-67025399 None:intergenic 25.0%
!!! TTTAGCTCGTTTAAAACTTT+TGG + chr3_4:67025382-67025401 Msa0515700:CDS 25.0%
! ACAAAACATTGTTCAAAACT+TGG + chr3_4:67025522-67025541 Msa0515700:CDS 25.0%
! TTACTTATTCAACCATTTCT+TGG - chr3_4:67025613-67025632 None:intergenic 25.0%
!! TTCTTTTCTTTTGACAATGA+AGG - chr3_4:67025890-67025909 None:intergenic 25.0%
TTCTTTCTTCTTTCTTCTTC+TGG + chr3_4:67023445-67023464 Msa0515700:five_prime_UTR 30.0%
TCTTTCTTCTTTCTTCTTCT+GGG + chr3_4:67023446-67023465 Msa0515700:five_prime_UTR 30.0%
! TGCTTTCACTTGTGTTTTAT+CGG + chr3_4:67023558-67023577 Msa0515700:intron 30.0%
GCATGTCAAATTTCTGTTAT+TGG + chr3_4:67023975-67023994 Msa0515700:intron 30.0%
TTCACATTATTCTGTCGTAA+AGG - chr3_4:67024188-67024207 None:intergenic 30.0%
!! GTTGTTGTTTATTGACAACT+TGG + chr3_4:67024242-67024261 Msa0515700:intron 30.0%
CAAAAATCACTCAGAACATT+GGG - chr3_4:67024291-67024310 None:intergenic 30.0%
ATATCATCATCCGTTTGATT+TGG + chr3_4:67024394-67024413 Msa0515700:CDS 30.0%
GAATAATGAAGAAGGTTTGA+AGG - chr3_4:67024610-67024629 None:intergenic 30.0%
TGATGACAGAATAATGAAGA+AGG - chr3_4:67024618-67024637 None:intergenic 30.0%
AAAGTTGAATATGCAGAAAG+CGG - chr3_4:67024769-67024788 None:intergenic 30.0%
CAATGTAGCATATACACAAT+AGG - chr3_4:67024826-67024845 None:intergenic 30.0%
! AAGCTTTTATCGAGAATTGA+TGG - chr3_4:67024864-67024883 None:intergenic 30.0%
CTTCAACATTTCATTCTTAG+TGG - chr3_4:67024905-67024924 None:intergenic 30.0%
TTTACGTTTCCAAGTTCTTA+AGG + chr3_4:67025132-67025151 Msa0515700:CDS 30.0%
! CAACTTTTCTTCAGTTTGAT+AGG - chr3_4:67025228-67025247 None:intergenic 30.0%
AGAATGAGTACATAACTATG+AGG + chr3_4:67025411-67025430 Msa0515700:CDS 30.0%
! GTGATTGCTAAACATTTTGA+TGG + chr3_4:67025488-67025507 Msa0515700:CDS 30.0%
AAATAAAAAGATAGGGCACA+CGG - chr3_4:67025684-67025703 None:intergenic 30.0%
CATTGTCAAAAGAAAAGAAG+TGG + chr3_4:67025891-67025910 Msa0515700:three_prime_UTR 30.0%
!!! AAGGTTTTAGGGATTGAAAG+TGG - chr3_4:67023415-67023434 None:intergenic 35.0%
! TCACTTGTGTTTTATCGGAT+TGG + chr3_4:67023563-67023582 Msa0515700:intron 35.0%
ACAATGTAAGAAAGATTGGC+AGG - chr3_4:67024141-67024160 None:intergenic 35.0%
CGAGACAATGTAAGAAAGAT+TGG - chr3_4:67024145-67024164 None:intergenic 35.0%
CCAAAAATCACTCAGAACAT+TGG - chr3_4:67024292-67024311 None:intergenic 35.0%
!! CCAATGTTCTGAGTGATTTT+TGG + chr3_4:67024289-67024308 Msa0515700:five_prime_UTR 35.0%
!!! TTCTGAGTGATTTTTGGTGT+TGG + chr3_4:67024295-67024314 Msa0515700:five_prime_UTR 35.0%
! GCATTTTATTATCACCACCT+AGG - chr3_4:67024370-67024389 None:intergenic 35.0%
AGACTTGCATTGGAAGAAAA+AGG + chr3_4:67024431-67024450 Msa0515700:CDS 35.0%
CGGATTCATCTGAAAGAATA+AGG - chr3_4:67024510-67024529 None:intergenic 35.0%
CTTCATTATTCTGTCATCAC+TGG + chr3_4:67024619-67024638 Msa0515700:intron 35.0%
ACAAATGTCTTACAGTGCTA+TGG + chr3_4:67024957-67024976 Msa0515700:intron 35.0%
! TACAGTGCTATGGATTTTTC+AGG + chr3_4:67024967-67024986 Msa0515700:intron 35.0%
GTACATAGAAAGAATTGCTG+TGG + chr3_4:67024989-67025008 Msa0515700:CDS 35.0%
ATAGGAGTGAAGTGATTGAA+TGG + chr3_4:67025042-67025061 Msa0515700:CDS 35.0%
AAGTGATTGAATGGATGAAG+AGG + chr3_4:67025051-67025070 Msa0515700:CDS 35.0%
TGGGAGAGGGAAAAATATTT+AGG - chr3_4:67025092-67025111 None:intergenic 35.0%
AGAACTTGGAAACGTAAACT+CGG - chr3_4:67025130-67025149 None:intergenic 35.0%
ACTGAGTGAAACACCTTATT+TGG + chr3_4:67025316-67025335 Msa0515700:CDS 35.0%
ATCCAATACTCAGCAATGTT+TGG - chr3_4:67025437-67025456 None:intergenic 35.0%
!!! CATTGCTGAGTATTGGATTT+TGG + chr3_4:67025439-67025458 Msa0515700:CDS 35.0%
GCAATCACCTTTCTATAACT+AGG - chr3_4:67025476-67025495 None:intergenic 35.0%
!! TTGCTAAACATTTTGATGGC+TGG + chr3_4:67025492-67025511 Msa0515700:CDS 35.0%
TTTGCTTAATCGGTACTAGT+AGG + chr3_4:67025565-67025584 Msa0515700:exon 35.0%
TAGGTGCATATATACTCTCA+TGG + chr3_4:67025584-67025603 Msa0515700:three_prime_UTR 35.0%
! TTTATTTCTCCCTTACTGCT+TGG + chr3_4:67025730-67025749 Msa0515700:three_prime_UTR 35.0%
CACTTATCCTAGAATGAGAA+GGG + chr3_4:67025780-67025799 Msa0515700:three_prime_UTR 35.0%
AATCAAGGTATGCATGTAAG+TGG + chr3_4:67025808-67025827 Msa0515700:three_prime_UTR 35.0%
GTTTGTCTCCAGAATTTCAT+TGG + chr3_4:67025830-67025849 Msa0515700:three_prime_UTR 35.0%
TACTTGGTCCAATGAAATTC+TGG - chr3_4:67025841-67025860 None:intergenic 35.0%
! CTTTTGACAATGAAGGAAGA+TGG - chr3_4:67025883-67025902 None:intergenic 35.0%
!! GTTATGTGTGACACTAGTTT+TGG + chr3_4:67026010-67026029 Msa0515700:three_prime_UTR 35.0%
TAGATCAAGAACGTAACCAA+TGG + chr3_4:67026066-67026085 Msa0515700:three_prime_UTR 35.0%
AAGTGGAGAGAGTGAGAAAA+AGG - chr3_4:67023398-67023417 None:intergenic 40.0%
! TATAGGCTTCTGTCACGTTT+TGG + chr3_4:67024102-67024121 Msa0515700:intron 40.0%
ATACCTATCAGCAACTTCCT+AGG + chr3_4:67024350-67024369 Msa0515700:five_prime_UTR 40.0%
! TTCTGGCTGTCCAAATCAAA+CGG - chr3_4:67024407-67024426 None:intergenic 40.0%
TTCCATACCAATCCTGTAAC+TGG + chr3_4:67024470-67024489 Msa0515700:CDS 40.0%
ATTCTTGCCAGTTACAGGAT+TGG - chr3_4:67024480-67024499 None:intergenic 40.0%
TCCTGTAACTGGCAAGAATT+TGG + chr3_4:67024481-67024500 Msa0515700:CDS 40.0%
TCCAAATTCTTGCCAGTTAC+AGG - chr3_4:67024485-67024504 None:intergenic 40.0%
TTCTTTCAGATGAATCCGAG+TGG + chr3_4:67024512-67024531 Msa0515700:CDS 40.0%
! AGCCTTCCTGTTTTCCAAAA+CGG + chr3_4:67024536-67024555 Msa0515700:CDS 40.0%
! AAATGATGTGAGAGCCGTTT+TGG - chr3_4:67024553-67024572 None:intergenic 40.0%
! ATAGGCTGCTTCAAGTTCAT+GGG - chr3_4:67024808-67024827 None:intergenic 40.0%
! AATAGGCTGCTTCAAGTTCA+TGG - chr3_4:67024809-67024828 None:intergenic 40.0%
CTGAGGATATCAGTAGCAAT+AGG + chr3_4:67025024-67025043 Msa0515700:CDS 40.0%
GATGAAGAGGATACAAGAGT+GGG + chr3_4:67025064-67025083 Msa0515700:CDS 40.0%
TTTCTCTGGTATATGGGAGA+GGG - chr3_4:67025105-67025124 None:intergenic 40.0%
! TCGGTGTTTCTCTGGTATAT+GGG - chr3_4:67025111-67025130 None:intergenic 40.0%
AGGCTTCTCTCAACTTTCTA+TGG - chr3_4:67025208-67025227 None:intergenic 40.0%
!!! AAGACCCTCATGTTTTGAGA+AGG + chr3_4:67025249-67025268 Msa0515700:CDS 40.0%
GGTTAGATTGCTTGATGAAG+CGG + chr3_4:67025286-67025305 Msa0515700:CDS 40.0%
! AGTGAAACACCTTATTTGGC+AGG + chr3_4:67025320-67025339 Msa0515700:CDS 40.0%
!!! ATTGGATTTTGGTTCAGCAG+AGG + chr3_4:67025450-67025469 Msa0515700:CDS 40.0%
CTAACATCACCAAGCAGTAA+GGG - chr3_4:67025742-67025761 None:intergenic 40.0%
ACTAACATCACCAAGCAGTA+AGG - chr3_4:67025743-67025762 None:intergenic 40.0%
GCACTTATCCTAGAATGAGA+AGG + chr3_4:67025779-67025798 Msa0515700:three_prime_UTR 40.0%
TTATCCTAGAATGAGAAGGG+AGG + chr3_4:67025783-67025802 Msa0515700:three_prime_UTR 40.0%
! TTTTCCTCCCTTCTCATTCT+AGG - chr3_4:67025790-67025809 None:intergenic 40.0%
ATGAGAAGGGAGGAAAATCA+AGG + chr3_4:67025793-67025812 Msa0515700:three_prime_UTR 40.0%
!! ACCAAGTACTGAAACAGTTC+AGG + chr3_4:67025853-67025872 Msa0515700:three_prime_UTR 40.0%
!! CCAAGTACTGAAACAGTTCA+GGG + chr3_4:67025854-67025873 Msa0515700:three_prime_UTR 40.0%
TGGAACAATGCCTTCTTGTA+AGG + chr3_4:67025977-67025996 Msa0515700:three_prime_UTR 40.0%
AAGCAGCAATCCTTACAAGA+AGG - chr3_4:67025990-67026009 None:intergenic 40.0%
GGGTGAAATAAAGTAGCCAT+TGG - chr3_4:67026085-67026104 None:intergenic 40.0%
!! GTTTGATTTGGACAGCCAGA+AGG + chr3_4:67024406-67024425 Msa0515700:CDS 45.0%
! TGCCAGTTACAGGATTGGTA+TGG - chr3_4:67024475-67024494 None:intergenic 45.0%
AAAACAGGAAGGCTTCCACT+CGG - chr3_4:67024530-67024549 None:intergenic 45.0%
!!! AGCCGTTTTGGAAAACAGGA+AGG - chr3_4:67024541-67024560 None:intergenic 45.0%
!!! TGAGAGCCGTTTTGGAAAAC+AGG - chr3_4:67024545-67024564 None:intergenic 45.0%
GGATGAAGAGGATACAAGAG+TGG + chr3_4:67025063-67025082 Msa0515700:CDS 45.0%
GTTTCTCTGGTATATGGGAG+AGG - chr3_4:67025106-67025125 None:intergenic 45.0%
!! CTCGGTGTTTCTCTGGTATA+TGG - chr3_4:67025112-67025131 None:intergenic 45.0%
!! ACGTAAACTCGGTGTTTCTC+TGG - chr3_4:67025119-67025138 None:intergenic 45.0%
TTCCAAGTTCTTAAGGCGTG+TGG + chr3_4:67025139-67025158 Msa0515700:CDS 45.0%
GTATGCACCTGCTAATTCAG+GGG - chr3_4:67025186-67025205 None:intergenic 45.0%
GGTATGCACCTGCTAATTCA+GGG - chr3_4:67025187-67025206 None:intergenic 45.0%
TGGTATGCACCTGCTAATTC+AGG - chr3_4:67025188-67025207 None:intergenic 45.0%
CGCTCCTTCTCAAAACATGA+GGG - chr3_4:67025256-67025275 None:intergenic 45.0%
TCGCTCCTTCTCAAAACATG+AGG - chr3_4:67025257-67025276 None:intergenic 45.0%
!!! TCATGTTTTGAGAAGGAGCG+AGG + chr3_4:67025256-67025275 Msa0515700:CDS 45.0%
AACTCTTCGCCTGCCAAATA+AGG - chr3_4:67025332-67025351 None:intergenic 45.0%
GGCCAAACATTGCTGAGTAT+TGG + chr3_4:67025432-67025451 Msa0515700:CDS 45.0%
GCAGAGGCCTAGTTATAGAA+AGG + chr3_4:67025466-67025485 Msa0515700:CDS 45.0%
CTCTCATGGTTGCCAAGAAA+TGG + chr3_4:67025598-67025617 Msa0515700:three_prime_UTR 45.0%
CCCTGAACTGTTTCAGTACT+TGG - chr3_4:67025857-67025876 None:intergenic 45.0%
!! AAAATTGATATATTAATAAA+TGG - chr3_4:67024059-67024078 None:intergenic 5.0%
CCACCTAGGAAGTTGCTGAT+AGG - chr3_4:67024356-67024375 None:intergenic 50.0%
CCTATCAGCAACTTCCTAGG+TGG + chr3_4:67024353-67024372 Msa0515700:five_prime_UTR 50.0%
CCAATGCAAGTCTCACCTTC+TGG - chr3_4:67024424-67024443 None:intergenic 50.0%
CCAGAAGGTGAGACTTGCAT+TGG + chr3_4:67024421-67024440 Msa0515700:CDS 50.0%
ACTGATATCCTCAGACCCTG+CGG - chr3_4:67025018-67025037 None:intergenic 50.0%
CACCACACGCCTTAAGAACT+TGG - chr3_4:67025144-67025163 None:intergenic 50.0%
TGTGGTGATCGCTCGTATGT+CGG + chr3_4:67025157-67025176 Msa0515700:CDS 50.0%
AGAATTGCTGTGGTCTCCGC+AGG + chr3_4:67024999-67025018 Msa0515700:CDS 55.0%
GAATTGCTGTGGTCTCCGCA+GGG + chr3_4:67025000-67025019 Msa0515700:CDS 55.0%
TCGGAGTCCCCTGAATTAGC+AGG + chr3_4:67025176-67025195 Msa0515700:CDS 55.0%
!! GAGAAGGAGCGAGGAGCATT+TGG + chr3_4:67025265-67025284 Msa0515700:CDS 55.0%
GGCAGGCGAAGAGTTTAGCA+TGG + chr3_4:67025337-67025356 Msa0515700:CDS 55.0%
TGTGGTCTCCGCAGGGTCTG+AGG + chr3_4:67025007-67025026 Msa0515700:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 67023352 67026108 67023352 ID=Msa0515700;Name=Msa0515700
chr3_4 mRNA 67023352 67026108 67023352 ID=Msa0515700-mRNA-1;Parent=Msa0515700;Name=Msa0515700-mRNA-1;_AED=0.17;_eAED=0.17;_QI=216|1|1|1|0.5|0.33|3|525|267
chr3_4 exon 67023352 67023467 67023352 ID=Msa0515700-mRNA-1:exon:17364;Parent=Msa0515700-mRNA-1
chr3_4 exon 67024286 67024594 67024286 ID=Msa0515700-mRNA-1:exon:17365;Parent=Msa0515700-mRNA-1
chr3_4 exon 67024989 67026108 67024989 ID=Msa0515700-mRNA-1:exon:17366;Parent=Msa0515700-mRNA-1
chr3_4 five_prime_UTR 67023352 67023467 67023352 ID=Msa0515700-mRNA-1:five_prime_utr;Parent=Msa0515700-mRNA-1
chr3_4 five_prime_UTR 67024286 67024385 67024286 ID=Msa0515700-mRNA-1:five_prime_utr;Parent=Msa0515700-mRNA-1
chr3_4 CDS 67024386 67024594 67024386 ID=Msa0515700-mRNA-1:cds;Parent=Msa0515700-mRNA-1
chr3_4 CDS 67024989 67025583 67024989 ID=Msa0515700-mRNA-1:cds;Parent=Msa0515700-mRNA-1
chr3_4 three_prime_UTR 67025584 67026108 67025584 ID=Msa0515700-mRNA-1:three_prime_utr;Parent=Msa0515700-mRNA-1
Gene Sequence

>Msa0515700

ATGCAGCTATATCATCATCCGTTTGATTTGGACAGCCAGAAGGTGAGACTTGCATTGGAAGAAAAAGGCATTGATTATACATCTTTCCATACCAATCCTGTAACTGGCAAGAATTTGGATTCCTTATTCTTTCAGATGAATCCGAGTGGAAGCCTTCCTGTTTTCCAAAACGGCTCTCACATCATTTACAAGACTATTGATATAATTCAGTACATAGAAAGAATTGCTGTGGTCTCCGCAGGGTCTGAGGATATCAGTAGCAATAGGAGTGAAGTGATTGAATGGATGAAGAGGATACAAGAGTGGGATCCTAAATATTTTTCCCTCTCCCATATACCAGAGAAACACCGAGTTTACGTTTCCAAGTTCTTAAGGCGTGTGGTGATCGCTCGTATGTCGGAGTCCCCTGAATTAGCAGGTGCATACCATAGAAAGTTGAGAGAAGCCTATCAAACTGAAGAAAAGTTGAAAGACCCTCATGTTTTGAGAAGGAGCGAGGAGCATTTGGTTAGATTGCTTGATGAAGCGGAAAGACAACTGAGTGAAACACCTTATTTGGCAGGCGAAGAGTTTAGCATGGCTGATGTGATGCTCATTCCGATTTTAGCTCGTTTAAAACTTTTGGATTTAGAGAATGAGTACATAACTATGAGGCCAAACATTGCTGAGTATTGGATTTTGGTTCAGCAGAGGCCTAGTTATAGAAAGGTGATTGCTAAACATTTTGATGGCTGGAGAAAGCACAAAACATTGTTCAAAACTTGGTGCTTTGTTCATATTAGAACTTTGCTTAATCGGTACTAG
Protein sequence

>Msa0515700

MQLYHHPFDLDSQKVRLALEEKGIDYTSFHTNPVTGKNLDSLFFQMNPSGSLPVFQNGSHIIYKTIDIIQYIERIAVVSAGSEDISSNRSEVIEWMKRIQEWDPKYFSLSHIPEKHRVYVSKFLRRVVIARMSESPELAGAYHRKLREAYQTEEKLKDPHVLRRSEEHLVRLLDEAERQLSETPYLAGEEFSMADVMLIPILARLKLLDLENEYITMRPNIAEYWILVQQRPSYRKVIAKHFDGWRKHKTLFKTWCFVHIRTLLNRY*