AlfalfaGEDB | Msa0425300

M. sativa cultivar zhongmu-4 / Msa0425300

KEGG: -
GO: -
PFAM: PF12937.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0425300	XP_003602304.1	98.148	162	3	0	1	162	1	162	8.53e-114	329

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0425300	G7J857	98.148	162	3	0	1	162	1	162	4.07e-114	329

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0425300	Msa0480600	0.844621	6.747502e-59	-8.615850e-47
Msa0425300	Msa1404670	0.847564	1.067806e-59	-8.615850e-47
Msa0425300	Msa1455390	0.844928	5.578870e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0425300	MtrunA17_Chr3g0127331	98.148	162	3	0	1	162	1	162	7.85e-118	329

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 40 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTAGCAACAGTTTCCT+TGG	0.390750	3_2:-75695739	None:intergenic
AATATATAATTTAATTATCA+AGG	0.424029	3_2:-75698009	None:intergenic
ACTTTCACTATTGGGTTGAA+TGG	0.462372	3_2:-75697818	None:intergenic
AGTTCAGAAACGGACTTCAC+TGG	0.455167	3_2:+75697671	Msa0425300:CDS
ATCATGGAAAGAAGTGTATG+AGG	0.545115	3_2:+75697063	Msa0425300:CDS
ATCTTCATACAGATTTCCAA+AGG	0.614774	3_2:-75695851	None:intergenic
ATTGGGTTGAATGGTAGAGA+TGG	0.590103	3_2:-75697809	None:intergenic
CAAAATGGAAACCTTACCTT+TGG	0.392712	3_2:+75695835	Msa0425300:exon
CAAAGGTAAGGTTTCCATTT+TGG	0.114718	3_2:-75695834	None:intergenic
CAAGATCGATGCGATCGAGT+TGG	0.561970	3_2:+75697088	Msa0425300:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTATAATCTAATCCCCAAAA+TGG	+	chr3_2:75695820-75695839	Msa0425300:exon	30.0%
ATCTTCATACAGATTTCCAA+AGG	-	chr3_2:75695854-75695873	None:intergenic	30.0%
TCTTTATCAGAAAACAGTGT+TGG	+	chr3_2:75696186-75696205	Msa0425300:intron	30.0%
AAATATGAATCAAGTCCATG+TGG	-	chr3_2:75696499-75696518	None:intergenic	30.0%
ATAGGTTAAAACTGACGATT+TGG	+	chr3_2:75696786-75696805	Msa0425300:intron	30.0%
TTTCTGCACACTTATAACAT+TGG	+	chr3_2:75697340-75697359	Msa0425300:intron	30.0%
ATTATTGAACTCTGACACAA+AGG	+	chr3_2:75697506-75697525	Msa0425300:intron	30.0%
TTATTGAACTCTGACACAAA+GGG	+	chr3_2:75697507-75697526	Msa0425300:intron	30.0%
GATCACATAAGTAGTGTTTA+TGG	+	chr3_2:75698071-75698090	Msa0425300:three_prime_UTR	30.0%
CTATAATCTAATCCCCAAAA+TGG	+	chr3_2:75695820-75695839	Msa0425300:exon	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	75695657	75698091	75695657	ID=Msa0425300;Name=Msa0425300
chr3_2	mRNA	75695657	75698091	75695657	ID=Msa0425300-mRNA-1;Parent=Msa0425300;Name=Msa0425300-mRNA-1;_AED=0.03;_eAED=0.03;_QI=182\|1\|1\|1\|1\|1\|3\|283\|162
chr3_2	exon	75695657	75695917	75695657	ID=Msa0425300-mRNA-1:exon:19413;Parent=Msa0425300-mRNA-1
chr3_2	exon	75696954	75697113	75696954	ID=Msa0425300-mRNA-1:exon:19414;Parent=Msa0425300-mRNA-1
chr3_2	exon	75697559	75698091	75697559	ID=Msa0425300-mRNA-1:exon:19415;Parent=Msa0425300-mRNA-1
chr3_2	five_prime_UTR	75695657	75695838	75695657	ID=Msa0425300-mRNA-1:five_prime_utr;Parent=Msa0425300-mRNA-1
chr3_2	CDS	75695839	75695917	75695839	ID=Msa0425300-mRNA-1:cds;Parent=Msa0425300-mRNA-1
chr3_2	CDS	75696954	75697113	75696954	ID=Msa0425300-mRNA-1:cds;Parent=Msa0425300-mRNA-1
chr3_2	CDS	75697559	75697808	75697559	ID=Msa0425300-mRNA-1:cds;Parent=Msa0425300-mRNA-1
chr3_2	three_prime_UTR	75697809	75698091	75697809	ID=Msa0425300-mRNA-1:three_prime_utr;Parent=Msa0425300-mRNA-1

Gene Sequence

>Msa0425300

ATGGAAACCTTACCTTTGGAAATCTGTATGAAGATATTCTGTTTCTTAGACCACACCCATCTTGCTGTTTCTCAACAAGTCTGTAGGAAGTGGAAGCTCATGGCCTCGGACAATGCTCTATGGTCAAACCTTTTTAAGGAGAGATGGGGAGGAGATCATGCTGCTTTTTATGCTCCTCTTGGTTCAAAATCATGGAAAGAAGTGTATGAGGCGCAAGATCGATGCGATCGAGTTGGATTGGGTTTGAAGATTGTTAGAGAAGGAAGTGACTACTATCTTGTTCACCAAGGTGAGGTACAACGATATCTAGGTTCAAGAAAAAATCGGAGACAAGTATCTGGTCATACTTCTAGTTCAGAAACGGACTTCACTGGAGAAAGTTTTGTAGCTGAAGAGAGATCATGTCGAGGAATCTTGGACAAAATTCTTTTTTTCATTGGGGATTTGGAAGCTGCTTCTGTTGATGCCAAACGCAGCCGTGTGATATAA

Protein sequence

>Msa0425300

METLPLEICMKIFCFLDHTHLAVSQQVCRKWKLMASDNALWSNLFKERWGGDHAAFYAPLGSKSWKEVYEAQDRCDRVGLGLKIVREGSDYYLVHQGEVQRYLGSRKNRRQVSGHTSSSETDFTGESFVAEERSCRGILDKILFFIGDLEAASVDAKRSRVI*