AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0425300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0425300 MtrunA17_Chr3g0127331 98.148 162 3 0 1 162 1 162 7.85e-118 329
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGTAAGGTTTCCATTT+TGG 0.114718 3_2:-75695834 None:intergenic
CTCCCCATCTCTCCTTAAAA+AGG 0.245866 3_2:-75697003 None:intergenic
CACTGTGAACTTTCACTATT+GGG 0.252264 3_2:-75697826 None:intergenic
CCACTGTGAACTTTCACTAT+TGG 0.288552 3_2:-75697827 None:intergenic
GTTATATCTTACTACAATAT+TGG 0.321352 3_2:+75697953 Msa0425300:three_prime_UTR
CTATAATCTAATCCCCAAAA+TGG 0.329374 3_2:+75695820 Msa0425300:exon
CACTCTATCTATCTGTCTTT+CGG 0.341213 3_2:-75695684 None:intergenic
AAAGGTAGCAACAGTTTCCT+TGG 0.390750 3_2:-75695739 None:intergenic
CAAAATGGAAACCTTACCTT+TGG 0.392712 3_2:+75695835 Msa0425300:exon
CTCCTCTTGGTTCAAAATCA+TGG 0.393681 3_2:+75697047 Msa0425300:CDS
GAGATCATGTCGAGGAATCT+TGG 0.402090 3_2:+75697715 Msa0425300:CDS
TAGAAGGAGGAAGCAGTTGT+TGG 0.420420 3_2:+75695652 None:intergenic
AATATATAATTTAATTATCA+AGG 0.424029 3_2:-75698009 None:intergenic
TGGCCTCGGACAATGCTCTA+TGG 0.432233 3_2:+75696975 Msa0425300:CDS
TTATATCACACGGCTGCGTT+TGG 0.432650 3_2:-75697786 None:intergenic
TAGAGTGTTAGATTAGAAAC+AGG 0.436604 3_2:+75695700 Msa0425300:five_prime_UTR
CATACAGATTTCCAAAGGTA+AGG 0.450513 3_2:-75695846 None:intergenic
AGTTCAGAAACGGACTTCAC+TGG 0.455167 3_2:+75697671 Msa0425300:CDS
ACTTTCACTATTGGGTTGAA+TGG 0.462372 3_2:-75697818 None:intergenic
GGTGAGGTACAACGATATCT+AGG 0.477811 3_2:+75697608 Msa0425300:CDS
GAAGTGGAAGCTCATGGCCT+CGG 0.508465 3_2:+75696961 Msa0425300:CDS
GGTTTGAAGATTGTTAGAGA+AGG 0.530829 3_2:+75697560 Msa0425300:CDS
TTGTTGAGAAACAGCAAGAT+GGG 0.537051 3_2:-75695894 None:intergenic
ATCATGGAAAGAAGTGTATG+AGG 0.545115 3_2:+75697063 Msa0425300:CDS
CATCTTGCTGTTTCTCAACA+AGG 0.545411 3_2:+75695896 Msa0425300:CDS
TCATACTTCTAGTTCAGAAA+CGG 0.559652 3_2:+75697661 Msa0425300:CDS
CAAGATCGATGCGATCGAGT+TGG 0.561970 3_2:+75697088 Msa0425300:CDS
ATTGGGTTGAATGGTAGAGA+TGG 0.590103 3_2:-75697809 None:intergenic
CTGTAGGAAGTGGAAGCTCA+TGG 0.592096 3_2:+75696955 Msa0425300:CDS
CTTGTTGAGAAACAGCAAGA+TGG 0.595023 3_2:-75695895 None:intergenic
ATCTTCATACAGATTTCCAA+AGG 0.614774 3_2:-75695851 None:intergenic
TTTAAGTTGTATTTCAGACA+AGG 0.626907 3_2:+75698049 Msa0425300:three_prime_UTR
GAGAAACAGCAAGATGGGTG+TGG 0.636024 3_2:-75695889 None:intergenic
GATATCGTTGTACCTCACCT+TGG 0.640965 3_2:-75697604 None:intergenic
CTATCTTGTTCACCAAGGTG+AGG 0.666547 3_2:+75697592 Msa0425300:CDS
GTAGAGATGGTTATATCACA+CGG 0.677630 3_2:-75697796 None:intergenic
TGACCATAGAGCATTGTCCG+AGG 0.683112 3_2:-75696978 None:intergenic
GCTGAAGAGAGATCATGTCG+AGG 0.706201 3_2:+75697707 Msa0425300:CDS
GACTACTATCTTGTTCACCA+AGG 0.711703 3_2:+75697587 Msa0425300:CDS
CCAATAGTGAAAGTTCACAG+TGG 0.758241 3_2:+75697827 Msa0425300:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAATTAATAATAATTT+TGG + chr3_2:75697276-75697295 Msa0425300:intron 0.0%
!!! TTATTAATTAATAATAATTT+TGG + chr3_2:75697276-75697295 Msa0425300:intron 0.0%
!!! AAAAAATTAACAAAATGTTT+TGG - chr3_2:75696742-75696761 None:intergenic 10.0%
!!! TTTTAGAAGAAAAAAAATAT+GGG - chr3_2:75697305-75697324 None:intergenic 10.0%
!!! AAAAAATTAACAAAATGTTT+TGG - chr3_2:75696742-75696761 None:intergenic 10.0%
!!! TTTTAGAAGAAAAAAAATAT+GGG - chr3_2:75697305-75697324 None:intergenic 10.0%
!! AATTGTATAACAAAATTTGT+TGG - chr3_2:75696327-75696346 None:intergenic 15.0%
!!! AACAATAAAGATTGAATTTT+AGG - chr3_2:75696535-75696554 None:intergenic 15.0%
!! AATTGAATCAATCATAATAT+TGG + chr3_2:75696641-75696660 Msa0425300:intron 15.0%
!!! GTTTTAGAAGAAAAAAAATA+TGG - chr3_2:75697306-75697325 None:intergenic 15.0%
!!! ATAATTTGTTATGTTGAATA+TGG - chr3_2:75697410-75697429 None:intergenic 15.0%
!!! TTTTTAATTCTTCACAAAAT+AGG + chr3_2:75697537-75697556 Msa0425300:intron 15.0%
!!! TTTTAATTCTTCACAAAATA+GGG + chr3_2:75697538-75697557 Msa0425300:intron 15.0%
!!! ACAAAATTCTTTTTTTCATT+GGG + chr3_2:75697738-75697757 Msa0425300:CDS 15.0%
!! AATTGTATAACAAAATTTGT+TGG - chr3_2:75696327-75696346 None:intergenic 15.0%
!!! AACAATAAAGATTGAATTTT+AGG - chr3_2:75696535-75696554 None:intergenic 15.0%
!! AATTGAATCAATCATAATAT+TGG + chr3_2:75696641-75696660 Msa0425300:intron 15.0%
!!! GTTTTAGAAGAAAAAAAATA+TGG - chr3_2:75697306-75697325 None:intergenic 15.0%
!!! ATAATTTGTTATGTTGAATA+TGG - chr3_2:75697410-75697429 None:intergenic 15.0%
!!! TTTTTAATTCTTCACAAAAT+AGG + chr3_2:75697537-75697556 Msa0425300:intron 15.0%
!!! TTTTAATTCTTCACAAAATA+GGG + chr3_2:75697538-75697557 Msa0425300:intron 15.0%
!!! ACAAAATTCTTTTTTTCATT+GGG + chr3_2:75697738-75697757 Msa0425300:CDS 15.0%
!! CAAGAATATAAAAATGAAGA+TGG - chr3_2:75695952-75695971 None:intergenic 20.0%
!!! ATTTGAAATTCATAAGTCAA+CGG - chr3_2:75696018-75696037 None:intergenic 20.0%
!!! AAATTTGTTGGATATCATAT+AGG - chr3_2:75696315-75696334 None:intergenic 20.0%
!!! TCAATCTTTATTGTTTTGAT+CGG + chr3_2:75696540-75696559 Msa0425300:intron 20.0%
!! TTGACTGTAATGATATAAAA+AGG - chr3_2:75696623-75696642 None:intergenic 20.0%
!! ATCAATCATAATATTGGATT+AGG + chr3_2:75696647-75696666 Msa0425300:intron 20.0%
!!! TTGTCAAATATCTTTTTAGA+GGG - chr3_2:75696849-75696868 None:intergenic 20.0%
!! TAAACTCAGAATAATCTAAA+CGG - chr3_2:75696888-75696907 None:intergenic 20.0%
!!! CTGAGTTTATTACATTATTT+TGG + chr3_2:75696899-75696918 Msa0425300:intron 20.0%
!! AAAACAATTTGTCAAATTCA+AGG - chr3_2:75696930-75696949 None:intergenic 20.0%
!! TTTAATTCTTCACAAAATAG+GGG + chr3_2:75697539-75697558 Msa0425300:intron 20.0%
!!! GACAAAATTCTTTTTTTCAT+TGG + chr3_2:75697737-75697756 Msa0425300:CDS 20.0%
!!! CAAAATTCTTTTTTTCATTG+GGG + chr3_2:75697739-75697758 Msa0425300:CDS 20.0%
!! GTTATATCTTACTACAATAT+TGG + chr3_2:75697953-75697972 Msa0425300:three_prime_UTR 20.0%
!! TATGTATGTATAAAAAACTG+TGG + chr3_2:75697979-75697998 Msa0425300:three_prime_UTR 20.0%
!! CAAGAATATAAAAATGAAGA+TGG - chr3_2:75695952-75695971 None:intergenic 20.0%
!!! ATTTGAAATTCATAAGTCAA+CGG - chr3_2:75696018-75696037 None:intergenic 20.0%
!!! AAATTTGTTGGATATCATAT+AGG - chr3_2:75696315-75696334 None:intergenic 20.0%
!!! TCAATCTTTATTGTTTTGAT+CGG + chr3_2:75696540-75696559 Msa0425300:intron 20.0%
!! TTGACTGTAATGATATAAAA+AGG - chr3_2:75696623-75696642 None:intergenic 20.0%
!! ATCAATCATAATATTGGATT+AGG + chr3_2:75696647-75696666 Msa0425300:intron 20.0%
!!! TTGTCAAATATCTTTTTAGA+GGG - chr3_2:75696849-75696868 None:intergenic 20.0%
!! TAAACTCAGAATAATCTAAA+CGG - chr3_2:75696888-75696907 None:intergenic 20.0%
!!! CTGAGTTTATTACATTATTT+TGG + chr3_2:75696899-75696918 Msa0425300:intron 20.0%
!! AAAACAATTTGTCAAATTCA+AGG - chr3_2:75696930-75696949 None:intergenic 20.0%
!! TTTAATTCTTCACAAAATAG+GGG + chr3_2:75697539-75697558 Msa0425300:intron 20.0%
!!! GACAAAATTCTTTTTTTCAT+TGG + chr3_2:75697737-75697756 Msa0425300:CDS 20.0%
!!! CAAAATTCTTTTTTTCATTG+GGG + chr3_2:75697739-75697758 Msa0425300:CDS 20.0%
!! GTTATATCTTACTACAATAT+TGG + chr3_2:75697953-75697972 Msa0425300:three_prime_UTR 20.0%
!! TATGTATGTATAAAAAACTG+TGG + chr3_2:75697979-75697998 Msa0425300:three_prime_UTR 20.0%
! GTTTCAAGAAAGAGAAAAAA+AGG - chr3_2:75695760-75695779 None:intergenic 25.0%
! AATAACAATATTTCAACCGA+TGG - chr3_2:75696053-75696072 None:intergenic 25.0%
! TTATTAGATCTGAATCTGTA+TGG + chr3_2:75696068-75696087 Msa0425300:intron 25.0%
! TTATTGCTTCAAATTGAACT+AGG - chr3_2:75696371-75696390 None:intergenic 25.0%
!! ACTGTGATTTTATGATACAT+TGG + chr3_2:75696576-75696595 Msa0425300:intron 25.0%
!! TTCCTTTTAATGTTACACAT+AGG + chr3_2:75696768-75696787 Msa0425300:intron 25.0%
! AACCTATGTGTAACATTAAA+AGG - chr3_2:75696773-75696792 None:intergenic 25.0%
!! CTTGTCAAATATCTTTTTAG+AGG - chr3_2:75696850-75696869 None:intergenic 25.0%
! TTAATTGCCATGATTATTGA+AGG + chr3_2:75697186-75697205 Msa0425300:intron 25.0%
! AAAATCTCCTTCAATAATCA+TGG - chr3_2:75697196-75697215 None:intergenic 25.0%
! ATCTAGGTTCAAGAAAAAAT+CGG + chr3_2:75697624-75697643 Msa0425300:CDS 25.0%
!!! TTTAAGTTGTATTTCAGACA+AGG + chr3_2:75698049-75698068 Msa0425300:three_prime_UTR 25.0%
! GTTTCAAGAAAGAGAAAAAA+AGG - chr3_2:75695760-75695779 None:intergenic 25.0%
! AATAACAATATTTCAACCGA+TGG - chr3_2:75696053-75696072 None:intergenic 25.0%
! TTATTAGATCTGAATCTGTA+TGG + chr3_2:75696068-75696087 Msa0425300:intron 25.0%
! TTATTGCTTCAAATTGAACT+AGG - chr3_2:75696371-75696390 None:intergenic 25.0%
!! ACTGTGATTTTATGATACAT+TGG + chr3_2:75696576-75696595 Msa0425300:intron 25.0%
!! TTCCTTTTAATGTTACACAT+AGG + chr3_2:75696768-75696787 Msa0425300:intron 25.0%
! AACCTATGTGTAACATTAAA+AGG - chr3_2:75696773-75696792 None:intergenic 25.0%
!! CTTGTCAAATATCTTTTTAG+AGG - chr3_2:75696850-75696869 None:intergenic 25.0%
! TTAATTGCCATGATTATTGA+AGG + chr3_2:75697186-75697205 Msa0425300:intron 25.0%
! AAAATCTCCTTCAATAATCA+TGG - chr3_2:75697196-75697215 None:intergenic 25.0%
! ATCTAGGTTCAAGAAAAAAT+CGG + chr3_2:75697624-75697643 Msa0425300:CDS 25.0%
!!! TTTAAGTTGTATTTCAGACA+AGG + chr3_2:75698049-75698068 Msa0425300:three_prime_UTR 25.0%
! TAGAGTGTTAGATTAGAAAC+AGG + chr3_2:75695700-75695719 Msa0425300:five_prime_UTR 30.0%
CTATAATCTAATCCCCAAAA+TGG + chr3_2:75695820-75695839 Msa0425300:exon 30.0%
! AAAGGTAAGGTTTCCATTTT+GGG - chr3_2:75695836-75695855 None:intergenic 30.0%
ATCTTCATACAGATTTCCAA+AGG - chr3_2:75695854-75695873 None:intergenic 30.0%
TCTTTATCAGAAAACAGTGT+TGG + chr3_2:75696186-75696205 Msa0425300:intron 30.0%
!!! TTTTGTTTTCTGCGAAGAAA+TGG + chr3_2:75696255-75696274 Msa0425300:intron 30.0%
AAATATGAATCAAGTCCATG+TGG - chr3_2:75696499-75696518 None:intergenic 30.0%
ATAGGTTAAAACTGACGATT+TGG + chr3_2:75696786-75696805 Msa0425300:intron 30.0%
!! AAAACTGACGATTTGGTATT+TGG + chr3_2:75696793-75696812 Msa0425300:intron 30.0%
!! CAAATTGTTTTACAGTCTGT+AGG + chr3_2:75696939-75696958 Msa0425300:intron 30.0%
! TCTATGGTCAAACCTTTTTA+AGG + chr3_2:75696991-75697010 Msa0425300:CDS 30.0%
!! AAACCTTTTTAAGGAGAGAT+GGG + chr3_2:75697000-75697019 Msa0425300:CDS 30.0%
TTTCTGCACACTTATAACAT+TGG + chr3_2:75697340-75697359 Msa0425300:intron 30.0%
ATTATTGAACTCTGACACAA+AGG + chr3_2:75697506-75697525 Msa0425300:intron 30.0%
TTATTGAACTCTGACACAAA+GGG + chr3_2:75697507-75697526 Msa0425300:intron 30.0%
!! TCATACTTCTAGTTCAGAAA+CGG + chr3_2:75697661-75697680 Msa0425300:CDS 30.0%
!!! TCTTTTTTTCATTGGGGATT+TGG + chr3_2:75697745-75697764 Msa0425300:CDS 30.0%
GATCACATAAGTAGTGTTTA+TGG + chr3_2:75698071-75698090 Msa0425300:three_prime_UTR 30.0%
! TAGAGTGTTAGATTAGAAAC+AGG + chr3_2:75695700-75695719 Msa0425300:five_prime_UTR 30.0%
CTATAATCTAATCCCCAAAA+TGG + chr3_2:75695820-75695839 Msa0425300:exon 30.0%
! AAAGGTAAGGTTTCCATTTT+GGG - chr3_2:75695836-75695855 None:intergenic 30.0%
ATCTTCATACAGATTTCCAA+AGG - chr3_2:75695854-75695873 None:intergenic 30.0%
TCTTTATCAGAAAACAGTGT+TGG + chr3_2:75696186-75696205 Msa0425300:intron 30.0%
!!! TTTTGTTTTCTGCGAAGAAA+TGG + chr3_2:75696255-75696274 Msa0425300:intron 30.0%
AAATATGAATCAAGTCCATG+TGG - chr3_2:75696499-75696518 None:intergenic 30.0%
ATAGGTTAAAACTGACGATT+TGG + chr3_2:75696786-75696805 Msa0425300:intron 30.0%
!! AAAACTGACGATTTGGTATT+TGG + chr3_2:75696793-75696812 Msa0425300:intron 30.0%
!! CAAATTGTTTTACAGTCTGT+AGG + chr3_2:75696939-75696958 Msa0425300:intron 30.0%
! TCTATGGTCAAACCTTTTTA+AGG + chr3_2:75696991-75697010 Msa0425300:CDS 30.0%
!! AAACCTTTTTAAGGAGAGAT+GGG + chr3_2:75697000-75697019 Msa0425300:CDS 30.0%
TTTCTGCACACTTATAACAT+TGG + chr3_2:75697340-75697359 Msa0425300:intron 30.0%
ATTATTGAACTCTGACACAA+AGG + chr3_2:75697506-75697525 Msa0425300:intron 30.0%
TTATTGAACTCTGACACAAA+GGG + chr3_2:75697507-75697526 Msa0425300:intron 30.0%
!! TCATACTTCTAGTTCAGAAA+CGG + chr3_2:75697661-75697680 Msa0425300:CDS 30.0%
!!! TCTTTTTTTCATTGGGGATT+TGG + chr3_2:75697745-75697764 Msa0425300:CDS 30.0%
CACTCTATCTATCTGTCTTT+CGG - chr3_2:75695687-75695706 None:intergenic 35.0%
!!! TTCCTTGGATTCTGTTTTTG+TGG - chr3_2:75695727-75695746 None:intergenic 35.0%
! AAGGTAAGGTTTCCATTTTG+GGG - chr3_2:75695835-75695854 None:intergenic 35.0%
! CAAAGGTAAGGTTTCCATTT+TGG - chr3_2:75695837-75695856 None:intergenic 35.0%
CAAAATGGAAACCTTACCTT+TGG + chr3_2:75695835-75695854 Msa0425300:exon 35.0%
CATACAGATTTCCAAAGGTA+AGG - chr3_2:75695849-75695868 None:intergenic 35.0%
TTGTTGAGAAACAGCAAGAT+GGG - chr3_2:75695897-75695916 None:intergenic 35.0%
AAATCAGTTGCATGTTCCAT+CGG + chr3_2:75696034-75696053 Msa0425300:intron 35.0%
! TTATCAGAAAACAGTGTTGG+TGG + chr3_2:75696189-75696208 Msa0425300:intron 35.0%
GGATTAACAATATGTGACAG+AGG - chr3_2:75696294-75696313 None:intergenic 35.0%
! CTGTGTTTCTTTGAAAAGCA+TGG - chr3_2:75696434-75696453 None:intergenic 35.0%
GGTCAAATTCCAGATCATAA+TGG - chr3_2:75696468-75696487 None:intergenic 35.0%
TTTGACCAAAATTTGCCACA+TGG + chr3_2:75696481-75696500 Msa0425300:intron 35.0%
ATTTGGCAACTGAGTGTTAA+CGG + chr3_2:75696810-75696829 Msa0425300:intron 35.0%
!! CAAACCTTTTTAAGGAGAGA+TGG + chr3_2:75696999-75697018 Msa0425300:CDS 35.0%
!! AACCTTTTTAAGGAGAGATG+GGG + chr3_2:75697001-75697020 Msa0425300:CDS 35.0%
! TTCCATGATTTTGAACCAAG+AGG - chr3_2:75697052-75697071 None:intergenic 35.0%
! ATCATGGAAAGAAGTGTATG+AGG + chr3_2:75697063-75697082 Msa0425300:CDS 35.0%
ATAATTGCAAGCGTGTGATT+AGG - chr3_2:75697230-75697249 None:intergenic 35.0%
ATTATTCTTGTCCCTTGAGA+TGG + chr3_2:75697245-75697264 Msa0425300:intron 35.0%
! TAATTTTCACGCCATCTCAA+GGG - chr3_2:75697259-75697278 None:intergenic 35.0%
! ATAATTTTCACGCCATCTCA+AGG - chr3_2:75697260-75697279 None:intergenic 35.0%
CACACTTATAACATTGGTGT+TGG + chr3_2:75697346-75697365 Msa0425300:intron 35.0%
CTAAAGCAAATCCAAGTAAG+AGG - chr3_2:75697375-75697394 None:intergenic 35.0%
! ACAATAGAAAGTCTTGTGTG+TGG + chr3_2:75697478-75697497 Msa0425300:intron 35.0%
GGTTTGAAGATTGTTAGAGA+AGG + chr3_2:75697560-75697579 Msa0425300:CDS 35.0%
AAAAATCGGAGACAAGTATC+TGG + chr3_2:75697638-75697657 Msa0425300:CDS 35.0%
GTAGAGATGGTTATATCACA+CGG - chr3_2:75697799-75697818 None:intergenic 35.0%
!! ACTTTCACTATTGGGTTGAA+TGG - chr3_2:75697821-75697840 None:intergenic 35.0%
CACTGTGAACTTTCACTATT+GGG - chr3_2:75697829-75697848 None:intergenic 35.0%
CACTCTATCTATCTGTCTTT+CGG - chr3_2:75695687-75695706 None:intergenic 35.0%
!!! TTCCTTGGATTCTGTTTTTG+TGG - chr3_2:75695727-75695746 None:intergenic 35.0%
! AAGGTAAGGTTTCCATTTTG+GGG - chr3_2:75695835-75695854 None:intergenic 35.0%
! CAAAGGTAAGGTTTCCATTT+TGG - chr3_2:75695837-75695856 None:intergenic 35.0%
CAAAATGGAAACCTTACCTT+TGG + chr3_2:75695835-75695854 Msa0425300:exon 35.0%
CATACAGATTTCCAAAGGTA+AGG - chr3_2:75695849-75695868 None:intergenic 35.0%
TTGTTGAGAAACAGCAAGAT+GGG - chr3_2:75695897-75695916 None:intergenic 35.0%
AAATCAGTTGCATGTTCCAT+CGG + chr3_2:75696034-75696053 Msa0425300:intron 35.0%
! TTATCAGAAAACAGTGTTGG+TGG + chr3_2:75696189-75696208 Msa0425300:intron 35.0%
GGATTAACAATATGTGACAG+AGG - chr3_2:75696294-75696313 None:intergenic 35.0%
! CTGTGTTTCTTTGAAAAGCA+TGG - chr3_2:75696434-75696453 None:intergenic 35.0%
GGTCAAATTCCAGATCATAA+TGG - chr3_2:75696468-75696487 None:intergenic 35.0%
TTTGACCAAAATTTGCCACA+TGG + chr3_2:75696481-75696500 Msa0425300:intron 35.0%
ATTTGGCAACTGAGTGTTAA+CGG + chr3_2:75696810-75696829 Msa0425300:intron 35.0%
!! CAAACCTTTTTAAGGAGAGA+TGG + chr3_2:75696999-75697018 Msa0425300:CDS 35.0%
!! AACCTTTTTAAGGAGAGATG+GGG + chr3_2:75697001-75697020 Msa0425300:CDS 35.0%
! TTCCATGATTTTGAACCAAG+AGG - chr3_2:75697052-75697071 None:intergenic 35.0%
! ATCATGGAAAGAAGTGTATG+AGG + chr3_2:75697063-75697082 Msa0425300:CDS 35.0%
ATAATTGCAAGCGTGTGATT+AGG - chr3_2:75697230-75697249 None:intergenic 35.0%
ATTATTCTTGTCCCTTGAGA+TGG + chr3_2:75697245-75697264 Msa0425300:intron 35.0%
! TAATTTTCACGCCATCTCAA+GGG - chr3_2:75697259-75697278 None:intergenic 35.0%
! ATAATTTTCACGCCATCTCA+AGG - chr3_2:75697260-75697279 None:intergenic 35.0%
CACACTTATAACATTGGTGT+TGG + chr3_2:75697346-75697365 Msa0425300:intron 35.0%
CTAAAGCAAATCCAAGTAAG+AGG - chr3_2:75697375-75697394 None:intergenic 35.0%
! ACAATAGAAAGTCTTGTGTG+TGG + chr3_2:75697478-75697497 Msa0425300:intron 35.0%
GGTTTGAAGATTGTTAGAGA+AGG + chr3_2:75697560-75697579 Msa0425300:CDS 35.0%
AAAAATCGGAGACAAGTATC+TGG + chr3_2:75697638-75697657 Msa0425300:CDS 35.0%
GTAGAGATGGTTATATCACA+CGG - chr3_2:75697799-75697818 None:intergenic 35.0%
!! ACTTTCACTATTGGGTTGAA+TGG - chr3_2:75697821-75697840 None:intergenic 35.0%
CACTGTGAACTTTCACTATT+GGG - chr3_2:75697829-75697848 None:intergenic 35.0%
GACCACAAAAACAGAATCCA+AGG + chr3_2:75695722-75695741 Msa0425300:five_prime_UTR 40.0%
AAAGGTAGCAACAGTTTCCT+TGG - chr3_2:75695742-75695761 None:intergenic 40.0%
CGTGCAACAAAAACAGTAAC+TGG - chr3_2:75695787-75695806 None:intergenic 40.0%
CTTGTTGAGAAACAGCAAGA+TGG - chr3_2:75695898-75695917 None:intergenic 40.0%
!! CATCTTGCTGTTTCTCAACA+AGG + chr3_2:75695896-75695915 Msa0425300:CDS 40.0%
! GTGTTGGTGGAATTGAAAAC+AGG + chr3_2:75696202-75696221 Msa0425300:intron 40.0%
GGTTCACAGAACACAGTTAT+TGG - chr3_2:75696350-75696369 None:intergenic 40.0%
AAACACACCGCTATGTTTCT+TGG + chr3_2:75696390-75696409 Msa0425300:intron 40.0%
AACACACCGCTATGTTTCTT+GGG + chr3_2:75696391-75696410 Msa0425300:intron 40.0%
! CAAGTCCATGTGGCAAATTT+TGG - chr3_2:75696489-75696508 None:intergenic 40.0%
GCAAATCAGCAAGACATATG+CGG - chr3_2:75696720-75696739 None:intergenic 40.0%
! GTTTTACAGTCTGTAGGAAG+TGG + chr3_2:75696945-75696964 Msa0425300:intron 40.0%
CTCCTCTTGGTTCAAAATCA+TGG + chr3_2:75697047-75697066 Msa0425300:CDS 40.0%
TTACTCTGCTGAGAGTAGTA+CGG + chr3_2:75697128-75697147 Msa0425300:intron 40.0%
GACTACTATCTTGTTCACCA+AGG + chr3_2:75697587-75697606 Msa0425300:CDS 40.0%
!! ATTGGGTTGAATGGTAGAGA+TGG - chr3_2:75697812-75697831 None:intergenic 40.0%
CCACTGTGAACTTTCACTAT+TGG - chr3_2:75697830-75697849 None:intergenic 40.0%
CCAATAGTGAAAGTTCACAG+TGG + chr3_2:75697827-75697846 Msa0425300:three_prime_UTR 40.0%
GACCACAAAAACAGAATCCA+AGG + chr3_2:75695722-75695741 Msa0425300:five_prime_UTR 40.0%
AAAGGTAGCAACAGTTTCCT+TGG - chr3_2:75695742-75695761 None:intergenic 40.0%
CGTGCAACAAAAACAGTAAC+TGG - chr3_2:75695787-75695806 None:intergenic 40.0%
CTTGTTGAGAAACAGCAAGA+TGG - chr3_2:75695898-75695917 None:intergenic 40.0%
!! CATCTTGCTGTTTCTCAACA+AGG + chr3_2:75695896-75695915 Msa0425300:CDS 40.0%
! GTGTTGGTGGAATTGAAAAC+AGG + chr3_2:75696202-75696221 Msa0425300:intron 40.0%
GGTTCACAGAACACAGTTAT+TGG - chr3_2:75696350-75696369 None:intergenic 40.0%
AAACACACCGCTATGTTTCT+TGG + chr3_2:75696390-75696409 Msa0425300:intron 40.0%
AACACACCGCTATGTTTCTT+GGG + chr3_2:75696391-75696410 Msa0425300:intron 40.0%
! CAAGTCCATGTGGCAAATTT+TGG - chr3_2:75696489-75696508 None:intergenic 40.0%
GCAAATCAGCAAGACATATG+CGG - chr3_2:75696720-75696739 None:intergenic 40.0%
! GTTTTACAGTCTGTAGGAAG+TGG + chr3_2:75696945-75696964 Msa0425300:intron 40.0%
CTCCTCTTGGTTCAAAATCA+TGG + chr3_2:75697047-75697066 Msa0425300:CDS 40.0%
TTACTCTGCTGAGAGTAGTA+CGG + chr3_2:75697128-75697147 Msa0425300:intron 40.0%
GACTACTATCTTGTTCACCA+AGG + chr3_2:75697587-75697606 Msa0425300:CDS 40.0%
!! ATTGGGTTGAATGGTAGAGA+TGG - chr3_2:75697812-75697831 None:intergenic 40.0%
CCACTGTGAACTTTCACTAT+TGG - chr3_2:75697830-75697849 None:intergenic 40.0%
CCAATAGTGAAAGTTCACAG+TGG + chr3_2:75697827-75697846 Msa0425300:three_prime_UTR 40.0%
GCATGGTGAAAGTTAGAGTC+TGG - chr3_2:75696417-75696436 None:intergenic 45.0%
! CCAGATCATAATGGAAGCTG+AGG - chr3_2:75696459-75696478 None:intergenic 45.0%
CCTCAGCTTCCATTATGATC+TGG + chr3_2:75696456-75696475 Msa0425300:intron 45.0%
CTCCCCATCTCTCCTTAAAA+AGG - chr3_2:75697006-75697025 None:intergenic 45.0%
!! CTTTTTAAGGAGAGATGGGG+AGG + chr3_2:75697004-75697023 Msa0425300:CDS 45.0%
!!! GCTGCTTTTTATGCTCCTCT+TGG + chr3_2:75697034-75697053 Msa0425300:CDS 45.0%
CTATCTTGTTCACCAAGGTG+AGG + chr3_2:75697592-75697611 Msa0425300:CDS 45.0%
GATATCGTTGTACCTCACCT+TGG - chr3_2:75697607-75697626 None:intergenic 45.0%
GGTGAGGTACAACGATATCT+AGG + chr3_2:75697608-75697627 Msa0425300:CDS 45.0%
! AGTTCAGAAACGGACTTCAC+TGG + chr3_2:75697671-75697690 Msa0425300:CDS 45.0%
GAGATCATGTCGAGGAATCT+TGG + chr3_2:75697715-75697734 Msa0425300:CDS 45.0%
TTATATCACACGGCTGCGTT+TGG - chr3_2:75697789-75697808 None:intergenic 45.0%
GCATGGTGAAAGTTAGAGTC+TGG - chr3_2:75696417-75696436 None:intergenic 45.0%
! CCAGATCATAATGGAAGCTG+AGG - chr3_2:75696459-75696478 None:intergenic 45.0%
CCTCAGCTTCCATTATGATC+TGG + chr3_2:75696456-75696475 Msa0425300:intron 45.0%
CTCCCCATCTCTCCTTAAAA+AGG - chr3_2:75697006-75697025 None:intergenic 45.0%
!! CTTTTTAAGGAGAGATGGGG+AGG + chr3_2:75697004-75697023 Msa0425300:CDS 45.0%
!!! GCTGCTTTTTATGCTCCTCT+TGG + chr3_2:75697034-75697053 Msa0425300:CDS 45.0%
CTATCTTGTTCACCAAGGTG+AGG + chr3_2:75697592-75697611 Msa0425300:CDS 45.0%
GATATCGTTGTACCTCACCT+TGG - chr3_2:75697607-75697626 None:intergenic 45.0%
GGTGAGGTACAACGATATCT+AGG + chr3_2:75697608-75697627 Msa0425300:CDS 45.0%
! AGTTCAGAAACGGACTTCAC+TGG + chr3_2:75697671-75697690 Msa0425300:CDS 45.0%
GAGATCATGTCGAGGAATCT+TGG + chr3_2:75697715-75697734 Msa0425300:CDS 45.0%
TTATATCACACGGCTGCGTT+TGG - chr3_2:75697789-75697808 None:intergenic 45.0%
!! AATATATAATTTAATTATCA+AGG - chr3_2:75698012-75698031 None:intergenic 5.0%
!! AATATATAATTTAATTATCA+AGG - chr3_2:75698012-75698031 None:intergenic 5.0%
GAGAAACAGCAAGATGGGTG+TGG - chr3_2:75695892-75695911 None:intergenic 50.0%
GTCTGGCCCAAGAAACATAG+CGG - chr3_2:75696400-75696419 None:intergenic 50.0%
CTGTAGGAAGTGGAAGCTCA+TGG + chr3_2:75696955-75696974 Msa0425300:CDS 50.0%
!! TGACCATAGAGCATTGTCCG+AGG - chr3_2:75696981-75697000 None:intergenic 50.0%
CAAGATCGATGCGATCGAGT+TGG + chr3_2:75697088-75697107 Msa0425300:CDS 50.0%
!! GGTGTTGGCTTCCTCTTACT+TGG + chr3_2:75697361-75697380 Msa0425300:intron 50.0%
GCTGAAGAGAGATCATGTCG+AGG + chr3_2:75697707-75697726 Msa0425300:CDS 50.0%
GAGAAACAGCAAGATGGGTG+TGG - chr3_2:75695892-75695911 None:intergenic 50.0%
GTCTGGCCCAAGAAACATAG+CGG - chr3_2:75696400-75696419 None:intergenic 50.0%
CTGTAGGAAGTGGAAGCTCA+TGG + chr3_2:75696955-75696974 Msa0425300:CDS 50.0%
!! TGACCATAGAGCATTGTCCG+AGG - chr3_2:75696981-75697000 None:intergenic 50.0%
CAAGATCGATGCGATCGAGT+TGG + chr3_2:75697088-75697107 Msa0425300:CDS 50.0%
!! GGTGTTGGCTTCCTCTTACT+TGG + chr3_2:75697361-75697380 Msa0425300:intron 50.0%
GCTGAAGAGAGATCATGTCG+AGG + chr3_2:75697707-75697726 Msa0425300:CDS 50.0%
GAAGTGGAAGCTCATGGCCT+CGG + chr3_2:75696961-75696980 Msa0425300:CDS 55.0%
TGGCCTCGGACAATGCTCTA+TGG + chr3_2:75696975-75696994 Msa0425300:CDS 55.0%
GAAGTGGAAGCTCATGGCCT+CGG + chr3_2:75696961-75696980 Msa0425300:CDS 55.0%
TGGCCTCGGACAATGCTCTA+TGG + chr3_2:75696975-75696994 Msa0425300:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 75695657 75698091 75695657 ID=Msa0425300;Name=Msa0425300
chr3_2 mRNA 75695657 75698091 75695657 ID=Msa0425300-mRNA-1;Parent=Msa0425300;Name=Msa0425300-mRNA-1;_AED=0.03;_eAED=0.03;_QI=182|1|1|1|1|1|3|283|162
chr3_2 exon 75695657 75695917 75695657 ID=Msa0425300-mRNA-1:exon:19413;Parent=Msa0425300-mRNA-1
chr3_2 exon 75696954 75697113 75696954 ID=Msa0425300-mRNA-1:exon:19414;Parent=Msa0425300-mRNA-1
chr3_2 exon 75697559 75698091 75697559 ID=Msa0425300-mRNA-1:exon:19415;Parent=Msa0425300-mRNA-1
chr3_2 five_prime_UTR 75695657 75695838 75695657 ID=Msa0425300-mRNA-1:five_prime_utr;Parent=Msa0425300-mRNA-1
chr3_2 CDS 75695839 75695917 75695839 ID=Msa0425300-mRNA-1:cds;Parent=Msa0425300-mRNA-1
chr3_2 CDS 75696954 75697113 75696954 ID=Msa0425300-mRNA-1:cds;Parent=Msa0425300-mRNA-1
chr3_2 CDS 75697559 75697808 75697559 ID=Msa0425300-mRNA-1:cds;Parent=Msa0425300-mRNA-1
chr3_2 three_prime_UTR 75697809 75698091 75697809 ID=Msa0425300-mRNA-1:three_prime_utr;Parent=Msa0425300-mRNA-1
Gene Sequence

>Msa0425300

ATGGAAACCTTACCTTTGGAAATCTGTATGAAGATATTCTGTTTCTTAGACCACACCCATCTTGCTGTTTCTCAACAAGTCTGTAGGAAGTGGAAGCTCATGGCCTCGGACAATGCTCTATGGTCAAACCTTTTTAAGGAGAGATGGGGAGGAGATCATGCTGCTTTTTATGCTCCTCTTGGTTCAAAATCATGGAAAGAAGTGTATGAGGCGCAAGATCGATGCGATCGAGTTGGATTGGGTTTGAAGATTGTTAGAGAAGGAAGTGACTACTATCTTGTTCACCAAGGTGAGGTACAACGATATCTAGGTTCAAGAAAAAATCGGAGACAAGTATCTGGTCATACTTCTAGTTCAGAAACGGACTTCACTGGAGAAAGTTTTGTAGCTGAAGAGAGATCATGTCGAGGAATCTTGGACAAAATTCTTTTTTTCATTGGGGATTTGGAAGCTGCTTCTGTTGATGCCAAACGCAGCCGTGTGATATAA
Protein sequence

>Msa0425300

METLPLEICMKIFCFLDHTHLAVSQQVCRKWKLMASDNALWSNLFKERWGGDHAAFYAPLGSKSWKEVYEAQDRCDRVGLGLKIVREGSDYYLVHQGEVQRYLGSRKNRRQVSGHTSSSETDFTGESFVAEERSCRGILDKILFFIGDLEAASVDAKRSRVI*