AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0480600


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0480600 MtrunA17_Chr3g0127331 98.765 162 2 0 1 162 1 162 5.16e-119 332
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGTAAGGTTTCCATTT+TGG 0.114718 3_3:-85906224 None:intergenic
CTCCCCATCTCTCCTTAAAA+AGG 0.245866 3_3:-85907384 None:intergenic
CACTGTGAACTTTCACTATT+GGG 0.252264 3_3:-85908247 None:intergenic
CCACTGTGAACTTTCACTAT+TGG 0.288552 3_3:-85908248 None:intergenic
GTTATATCTTACTACAATAT+TGG 0.321352 3_3:+85908374 Msa0480600:three_prime_UTR
CTATAATCTAATCCCCAAAA+TGG 0.329374 3_3:+85906210 Msa0480600:exon
CACTCTATCTATCTGTCTTT+CGG 0.341213 3_3:-85906074 None:intergenic
AAAGGTAGCAACAGTTTCCT+TGG 0.390750 3_3:-85906129 None:intergenic
CAAAATGGAAACCTTACCTT+TGG 0.392712 3_3:+85906225 Msa0480600:exon
CTCCTCTTGGTTCAAAATCA+TGG 0.393681 3_3:+85907428 Msa0480600:CDS
GAGATCATGTCGAGGAATCT+TGG 0.402090 3_3:+85908136 Msa0480600:CDS
TAGAAGGAGGAAGCAGTTGT+TGG 0.420420 3_3:+85906042 None:intergenic
AATATATAATTTAATTATCA+AGG 0.424029 3_3:-85908430 None:intergenic
TGGCCTCGGACAATGCTCTA+TGG 0.432233 3_3:+85907356 Msa0480600:CDS
TTATATCACACGGCTGCGTT+TGG 0.432650 3_3:-85908207 None:intergenic
TAGAGTGTTAGATTAGAAAC+AGG 0.436604 3_3:+85906090 Msa0480600:five_prime_UTR
CATACAGATTTCCAAAGGTA+AGG 0.450513 3_3:-85906236 None:intergenic
ACTTTCACTATTGGGTTGAA+TGG 0.462372 3_3:-85908239 None:intergenic
GGTGAGGTACAACGATATCT+AGG 0.477811 3_3:+85908029 Msa0480600:CDS
AGTTCAGAAACAGACTTCAC+TGG 0.488792 3_3:+85908092 Msa0480600:CDS
GAAGTGGAAGCTCATGGCCT+CGG 0.508465 3_3:+85907342 Msa0480600:CDS
GGTTTGAAGATTGTTAGAGA+AGG 0.530829 3_3:+85907981 Msa0480600:CDS
TTGTTGAGAAACAGCAAGAT+GGG 0.537051 3_3:-85906284 None:intergenic
ATCATGGAAAGAAGTGTATG+AGG 0.545115 3_3:+85907444 Msa0480600:CDS
CATCTTGCTGTTTCTCAACA+AGG 0.545411 3_3:+85906286 Msa0480600:CDS
CAAGATCGATGCGATCGAGT+TGG 0.561970 3_3:+85907469 Msa0480600:CDS
ATTGGGTTGAATGGTAGAGA+TGG 0.590103 3_3:-85908230 None:intergenic
CTGTAGGAAGTGGAAGCTCA+TGG 0.592096 3_3:+85907336 Msa0480600:CDS
CTTGTTGAGAAACAGCAAGA+TGG 0.595023 3_3:-85906285 None:intergenic
ATCTTCATACAGATTTCCAA+AGG 0.614774 3_3:-85906241 None:intergenic
TTTAAGTTGTATTTCAGACA+AGG 0.626907 3_3:+85908470 Msa0480600:three_prime_UTR
GAGAAACAGCAAGATGGGTG+TGG 0.636024 3_3:-85906279 None:intergenic
GATATCGTTGTACCTCACCT+TGG 0.640965 3_3:-85908025 None:intergenic
CTATCTTGTTCACCAAGGTG+AGG 0.666547 3_3:+85908013 Msa0480600:CDS
GTAGAGATGGTTATATCACA+CGG 0.677630 3_3:-85908217 None:intergenic
TGACCATAGAGCATTGTCCG+AGG 0.683112 3_3:-85907359 None:intergenic
GCTGAAGAGAGATCATGTCG+AGG 0.706201 3_3:+85908128 Msa0480600:CDS
GACTACTATCTTGTTCACCA+AGG 0.711703 3_3:+85908008 Msa0480600:CDS
CCAATAGTGAAAGTTCACAG+TGG 0.758241 3_3:+85908248 Msa0480600:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAATTAATAATAATTT+TGG + chr3_3:85907657-85907676 Msa0480600:intron 0.0%
!!! AAAAAATTAACAAATTGTTT+TGG - chr3_3:85907123-85907142 None:intergenic 10.0%
!! AATTGTATAACAAAATTTGT+TGG - chr3_3:85906708-85906727 None:intergenic 15.0%
!!! AACAATAAAGATTGAATTTT+AGG - chr3_3:85906916-85906935 None:intergenic 15.0%
!! AATTGAATCAATCATAATAT+TGG + chr3_3:85907022-85907041 Msa0480600:intron 15.0%
!!! TTTTAGAAGGAAAAAAATAT+GGG - chr3_3:85907686-85907705 None:intergenic 15.0%
!!! ATAATTTGTTATGTTGAATA+TGG - chr3_3:85907826-85907845 None:intergenic 15.0%
!!! TTTTTAATTCTTCACAAAAT+AGG + chr3_3:85907958-85907977 Msa0480600:intron 15.0%
!!! TTTTAATTCTTCACAAAATA+GGG + chr3_3:85907959-85907978 Msa0480600:intron 15.0%
!!! ACAAAATTCTTTTTTTCATT+GGG + chr3_3:85908159-85908178 Msa0480600:CDS 15.0%
!! CAAGAATATAAAAATGAAGA+TGG - chr3_3:85906342-85906361 None:intergenic 20.0%
!!! ATTTGAAATTCATAAGTCAA+CGG - chr3_3:85906408-85906427 None:intergenic 20.0%
!! TTATTGCTTCAAATTAAACT+AGG - chr3_3:85906752-85906771 None:intergenic 20.0%
!!! TCAATCTTTATTGTTTTGAT+CGG + chr3_3:85906921-85906940 Msa0480600:intron 20.0%
!! TTGACTGTAATGATATAAAA+AGG - chr3_3:85907004-85907023 None:intergenic 20.0%
!! ATCAATCATAATATTGGATT+AGG + chr3_3:85907028-85907047 Msa0480600:intron 20.0%
!!! TTGTCAAATATCTTTTTAGA+GGG - chr3_3:85907230-85907249 None:intergenic 20.0%
!!! CTGAGTTTATTACATTATTT+TGG + chr3_3:85907280-85907299 Msa0480600:intron 20.0%
!! AAAACAATTTGTCAAATTCA+AGG - chr3_3:85907311-85907330 None:intergenic 20.0%
!!! GTTTTAGAAGGAAAAAAATA+TGG - chr3_3:85907687-85907706 None:intergenic 20.0%
!! TTTAAGAGAACTTATAACAT+TGG + chr3_3:85907756-85907775 Msa0480600:intron 20.0%
!! TTTAATTCTTCACAAAATAG+GGG + chr3_3:85907960-85907979 Msa0480600:intron 20.0%
!!! GACAAAATTCTTTTTTTCAT+TGG + chr3_3:85908158-85908177 Msa0480600:CDS 20.0%
!!! CAAAATTCTTTTTTTCATTG+GGG + chr3_3:85908160-85908179 Msa0480600:CDS 20.0%
!! GTTATATCTTACTACAATAT+TGG + chr3_3:85908374-85908393 Msa0480600:three_prime_UTR 20.0%
!! TATGTATGTATAAAAAACTG+TGG + chr3_3:85908400-85908419 Msa0480600:three_prime_UTR 20.0%
! GTTTCAAGAAAGAGAAAAAA+AGG - chr3_3:85906150-85906169 None:intergenic 25.0%
! AATAACAATATTTCAACCGA+TGG - chr3_3:85906443-85906462 None:intergenic 25.0%
! TTATTAGATCTGAATCTGTA+TGG + chr3_3:85906458-85906477 Msa0480600:intron 25.0%
! TGTTGGATATCATATGATAT+AGG - chr3_3:85906691-85906710 None:intergenic 25.0%
!! ACTGTGATTTTATGATACAT+TGG + chr3_3:85906957-85906976 Msa0480600:intron 25.0%
!! TTCCTTTTAATGTTACACAT+AGG + chr3_3:85907149-85907168 Msa0480600:intron 25.0%
! AACCTATGTGTAACATTAAA+AGG - chr3_3:85907154-85907173 None:intergenic 25.0%
!! CTTGTCAAATATCTTTTTAG+AGG - chr3_3:85907231-85907250 None:intergenic 25.0%
! TTAAATGCCATGATTATTGA+AGG + chr3_3:85907567-85907586 Msa0480600:intron 25.0%
! AAAATCTCCTTCAATAATCA+TGG - chr3_3:85907577-85907596 None:intergenic 25.0%
!!! AGCAATTATGTTGTTTTAGA+AGG - chr3_3:85907699-85907718 None:intergenic 25.0%
! ATCTAGGTTCAAGAAAAAAT+CGG + chr3_3:85908045-85908064 Msa0480600:CDS 25.0%
!!! TTTAAGTTGTATTTCAGACA+AGG + chr3_3:85908470-85908489 Msa0480600:three_prime_UTR 25.0%
! TAGAGTGTTAGATTAGAAAC+AGG + chr3_3:85906090-85906109 Msa0480600:five_prime_UTR 30.0%
CTATAATCTAATCCCCAAAA+TGG + chr3_3:85906210-85906229 Msa0480600:exon 30.0%
! AAAGGTAAGGTTTCCATTTT+GGG - chr3_3:85906226-85906245 None:intergenic 30.0%
ATCTTCATACAGATTTCCAA+AGG - chr3_3:85906244-85906263 None:intergenic 30.0%
TCTTTATCAGAAAACAGTGT+TGG + chr3_3:85906562-85906581 Msa0480600:intron 30.0%
!!! TTTTGTTTTCTGCGAAGAAA+TGG + chr3_3:85906631-85906650 Msa0480600:intron 30.0%
AAATATGAATCAAGTCCATG+TGG - chr3_3:85906880-85906899 None:intergenic 30.0%
ATAGGTTAAAACCGACTATT+TGG + chr3_3:85907167-85907186 Msa0480600:intron 30.0%
AAAACCGACTATTTGGTATT+TGG + chr3_3:85907174-85907193 Msa0480600:intron 30.0%
!! CAAATTGTTTTACAGTCTGT+AGG + chr3_3:85907320-85907339 Msa0480600:intron 30.0%
! TCTATGGTCAAACCTTTTTA+AGG + chr3_3:85907372-85907391 Msa0480600:CDS 30.0%
!! AAACCTTTTTAAGGAGAGAT+GGG + chr3_3:85907381-85907400 Msa0480600:CDS 30.0%
! TAATTTTCATGCCATCTCAA+GGG - chr3_3:85907640-85907659 None:intergenic 30.0%
! ATAATTTTCATGCCATCTCA+AGG - chr3_3:85907641-85907660 None:intergenic 30.0%
TTTCTGCACACTTATAACAT+TGG + chr3_3:85907721-85907740 Msa0480600:intron 30.0%
AGAACTTATAACATTGGTGT+TGG + chr3_3:85907762-85907781 Msa0480600:intron 30.0%
ATTATTGAACTCTGACACAA+AGG + chr3_3:85907927-85907946 Msa0480600:intron 30.0%
TTATTGAACTCTGACACAAA+GGG + chr3_3:85907928-85907947 Msa0480600:intron 30.0%
!!! TCTTTTTTTCATTGGGGATT+TGG + chr3_3:85908166-85908185 Msa0480600:CDS 30.0%
CACTCTATCTATCTGTCTTT+CGG - chr3_3:85906077-85906096 None:intergenic 35.0%
!!! TTCCTTGGATTCTGTTTTTG+TGG - chr3_3:85906117-85906136 None:intergenic 35.0%
! AAGGTAAGGTTTCCATTTTG+GGG - chr3_3:85906225-85906244 None:intergenic 35.0%
! CAAAGGTAAGGTTTCCATTT+TGG - chr3_3:85906227-85906246 None:intergenic 35.0%
CAAAATGGAAACCTTACCTT+TGG + chr3_3:85906225-85906244 Msa0480600:exon 35.0%
CATACAGATTTCCAAAGGTA+AGG - chr3_3:85906239-85906258 None:intergenic 35.0%
TTGTTGAGAAACAGCAAGAT+GGG - chr3_3:85906287-85906306 None:intergenic 35.0%
AAATCAGTTGCATGTTCCAT+CGG + chr3_3:85906424-85906443 Msa0480600:intron 35.0%
! TTATCAGAAAACAGTGTTGG+TGG + chr3_3:85906565-85906584 Msa0480600:intron 35.0%
GGATTAACAATATGTGACAG+AGG - chr3_3:85906670-85906689 None:intergenic 35.0%
! CTGTGTTTCTTTGAAAAGCA+TGG - chr3_3:85906815-85906834 None:intergenic 35.0%
GGTCAAATTCCAGATCATAA+TGG - chr3_3:85906849-85906868 None:intergenic 35.0%
TTTGACCAAAATTTGCCACA+TGG + chr3_3:85906862-85906881 Msa0480600:intron 35.0%
GTTGCCAAATACCAAATAGT+CGG - chr3_3:85907181-85907200 None:intergenic 35.0%
ATTTGGCAACTGAGTGTTAA+CGG + chr3_3:85907191-85907210 Msa0480600:intron 35.0%
!! CAAACCTTTTTAAGGAGAGA+TGG + chr3_3:85907380-85907399 Msa0480600:CDS 35.0%
!! AACCTTTTTAAGGAGAGATG+GGG + chr3_3:85907382-85907401 Msa0480600:CDS 35.0%
! TTCCATGATTTTGAACCAAG+AGG - chr3_3:85907433-85907452 None:intergenic 35.0%
! ATCATGGAAAGAAGTGTATG+AGG + chr3_3:85907444-85907463 Msa0480600:CDS 35.0%
ATAATTGCAAGCGTGTGATT+AGG - chr3_3:85907611-85907630 None:intergenic 35.0%
ATTATTCTTGTCCCTTGAGA+TGG + chr3_3:85907626-85907645 Msa0480600:intron 35.0%
CTAAAGCAAATCCAAGTAAG+AGG - chr3_3:85907791-85907810 None:intergenic 35.0%
! ACAATAGAAAGTCTTGTGTG+TGG + chr3_3:85907899-85907918 Msa0480600:intron 35.0%
GGTTTGAAGATTGTTAGAGA+AGG + chr3_3:85907981-85908000 Msa0480600:CDS 35.0%
GTAGAGATGGTTATATCACA+CGG - chr3_3:85908220-85908239 None:intergenic 35.0%
!! ACTTTCACTATTGGGTTGAA+TGG - chr3_3:85908242-85908261 None:intergenic 35.0%
CACTGTGAACTTTCACTATT+GGG - chr3_3:85908250-85908269 None:intergenic 35.0%
GACCACAAAAACAGAATCCA+AGG + chr3_3:85906112-85906131 Msa0480600:five_prime_UTR 40.0%
AAAGGTAGCAACAGTTTCCT+TGG - chr3_3:85906132-85906151 None:intergenic 40.0%
CGTGCAACAAAAACAGTAAC+TGG - chr3_3:85906177-85906196 None:intergenic 40.0%
CTTGTTGAGAAACAGCAAGA+TGG - chr3_3:85906288-85906307 None:intergenic 40.0%
!! CATCTTGCTGTTTCTCAACA+AGG + chr3_3:85906286-85906305 Msa0480600:CDS 40.0%
! GTGTTGGTGGAATTGAAAAC+AGG + chr3_3:85906578-85906597 Msa0480600:intron 40.0%
GGTTCACAGAACACAGTTAT+TGG - chr3_3:85906731-85906750 None:intergenic 40.0%
AAACACACCGCTATGTTTCT+TGG + chr3_3:85906771-85906790 Msa0480600:intron 40.0%
AACACACCGCTATGTTTCTT+GGG + chr3_3:85906772-85906791 Msa0480600:intron 40.0%
! CAAGTCCATGTGGCAAATTT+TGG - chr3_3:85906870-85906889 None:intergenic 40.0%
GCAAATCAGCAAGACATATG+CGG - chr3_3:85907101-85907120 None:intergenic 40.0%
! GTTTTACAGTCTGTAGGAAG+TGG + chr3_3:85907326-85907345 Msa0480600:intron 40.0%
CTCCTCTTGGTTCAAAATCA+TGG + chr3_3:85907428-85907447 Msa0480600:CDS 40.0%
TTACTCTGCTGAGAGTAGTA+CGG + chr3_3:85907509-85907528 Msa0480600:intron 40.0%
GACTACTATCTTGTTCACCA+AGG + chr3_3:85908008-85908027 Msa0480600:CDS 40.0%
AAAAATCGGCGACAAGTATC+TGG + chr3_3:85908059-85908078 Msa0480600:CDS 40.0%
AGTTCAGAAACAGACTTCAC+TGG + chr3_3:85908092-85908111 Msa0480600:CDS 40.0%
!! ATTGGGTTGAATGGTAGAGA+TGG - chr3_3:85908233-85908252 None:intergenic 40.0%
CCACTGTGAACTTTCACTAT+TGG - chr3_3:85908251-85908270 None:intergenic 40.0%
CCAATAGTGAAAGTTCACAG+TGG + chr3_3:85908248-85908267 Msa0480600:three_prime_UTR 40.0%
GCATGGTGAAAGTTAGAGTC+TGG - chr3_3:85906798-85906817 None:intergenic 45.0%
! CCAGATCATAATGGAAGCTG+AGG - chr3_3:85906840-85906859 None:intergenic 45.0%
CCTCAGCTTCCATTATGATC+TGG + chr3_3:85906837-85906856 Msa0480600:intron 45.0%
CTCCCCATCTCTCCTTAAAA+AGG - chr3_3:85907387-85907406 None:intergenic 45.0%
!! CTTTTTAAGGAGAGATGGGG+AGG + chr3_3:85907385-85907404 Msa0480600:CDS 45.0%
!!! GCTGCTTTTTATGCTCCTCT+TGG + chr3_3:85907415-85907434 Msa0480600:CDS 45.0%
CTATCTTGTTCACCAAGGTG+AGG + chr3_3:85908013-85908032 Msa0480600:CDS 45.0%
GATATCGTTGTACCTCACCT+TGG - chr3_3:85908028-85908047 None:intergenic 45.0%
GGTGAGGTACAACGATATCT+AGG + chr3_3:85908029-85908048 Msa0480600:CDS 45.0%
GAGATCATGTCGAGGAATCT+TGG + chr3_3:85908136-85908155 Msa0480600:CDS 45.0%
TTATATCACACGGCTGCGTT+TGG - chr3_3:85908210-85908229 None:intergenic 45.0%
!! AATATATAATTTAATTATCA+AGG - chr3_3:85908433-85908452 None:intergenic 5.0%
GAGAAACAGCAAGATGGGTG+TGG - chr3_3:85906282-85906301 None:intergenic 50.0%
GTCTGGCCCAAGAAACATAG+CGG - chr3_3:85906781-85906800 None:intergenic 50.0%
CTGTAGGAAGTGGAAGCTCA+TGG + chr3_3:85907336-85907355 Msa0480600:CDS 50.0%
!! TGACCATAGAGCATTGTCCG+AGG - chr3_3:85907362-85907381 None:intergenic 50.0%
CAAGATCGATGCGATCGAGT+TGG + chr3_3:85907469-85907488 Msa0480600:CDS 50.0%
!! GGTGTTGGCTTCCTCTTACT+TGG + chr3_3:85907777-85907796 Msa0480600:intron 50.0%
GCTGAAGAGAGATCATGTCG+AGG + chr3_3:85908128-85908147 Msa0480600:CDS 50.0%
GAAGTGGAAGCTCATGGCCT+CGG + chr3_3:85907342-85907361 Msa0480600:CDS 55.0%
TGGCCTCGGACAATGCTCTA+TGG + chr3_3:85907356-85907375 Msa0480600:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 85906047 85908512 85906047 ID=Msa0480600;Name=Msa0480600
chr3_3 mRNA 85906047 85908512 85906047 ID=Msa0480600-mRNA-1;Parent=Msa0480600;Name=Msa0480600-mRNA-1;_AED=0.03;_eAED=0.03;_QI=182|1|1|1|1|1|3|283|162
chr3_3 exon 85906047 85906307 85906047 ID=Msa0480600-mRNA-1:exon:24772;Parent=Msa0480600-mRNA-1
chr3_3 exon 85907335 85907494 85907335 ID=Msa0480600-mRNA-1:exon:24773;Parent=Msa0480600-mRNA-1
chr3_3 exon 85907980 85908512 85907980 ID=Msa0480600-mRNA-1:exon:24774;Parent=Msa0480600-mRNA-1
chr3_3 five_prime_UTR 85906047 85906228 85906047 ID=Msa0480600-mRNA-1:five_prime_utr;Parent=Msa0480600-mRNA-1
chr3_3 CDS 85906229 85906307 85906229 ID=Msa0480600-mRNA-1:cds;Parent=Msa0480600-mRNA-1
chr3_3 CDS 85907335 85907494 85907335 ID=Msa0480600-mRNA-1:cds;Parent=Msa0480600-mRNA-1
chr3_3 CDS 85907980 85908229 85907980 ID=Msa0480600-mRNA-1:cds;Parent=Msa0480600-mRNA-1
chr3_3 three_prime_UTR 85908230 85908512 85908230 ID=Msa0480600-mRNA-1:three_prime_utr;Parent=Msa0480600-mRNA-1
Gene Sequence

>Msa0480600

ATGGAAACCTTACCTTTGGAAATCTGTATGAAGATATTCTGTTTCTTAGACCACACCCATCTTGCTGTTTCTCAACAAGTCTGTAGGAAGTGGAAGCTCATGGCCTCGGACAATGCTCTATGGTCAAACCTTTTTAAGGAGAGATGGGGAGGAGATCATGCTGCTTTTTATGCTCCTCTTGGTTCAAAATCATGGAAAGAAGTGTATGAGGCGCAAGATCGATGCGATCGAGTTGGATTGGGTTTGAAGATTGTTAGAGAAGGAAGTGACTACTATCTTGTTCACCAAGGTGAGGTACAACGATATCTAGGTTCAAGAAAAAATCGGCGACAAGTATCTGGTCATACTTCTAGTTCAGAAACAGACTTCACTGGAGAAAGTTCTGTAGCTGAAGAGAGATCATGTCGAGGAATCTTGGACAAAATTCTTTTTTTCATTGGGGATTTGGAAGCTGCTTCTGTTGATGCCAAACGCAGCCGTGTGATATAA

Protein sequence

>Msa0480600

METLPLEICMKIFCFLDHTHLAVSQQVCRKWKLMASDNALWSNLFKERWGGDHAAFYAPLGSKSWKEVYEAQDRCDRVGLGLKIVREGSDYYLVHQGEVQRYLGSRKNRRQVSGHTSSSETDFTGESSVAEERSCRGILDKILFFIGDLEAASVDAKRSRVI*