AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0428630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 80 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCGGCTGCTTGGTAATT+TGG 0.141074 3_2:-79580175 Msa0428630:three_prime_UTR
AGACTTGGCTTCTTTATTTA+TGG 0.146631 3_2:-79580232 Msa0428630:three_prime_UTR
CACTTCTGCACCAAACATTT+TGG 0.189073 3_2:+79580633 None:intergenic
CTTCGGCTGCTTGGTAATTT+GGG 0.194034 3_2:-79580174 Msa0428630:three_prime_UTR
ACAACTTCTCAAATCTTTAC+AGG 0.209090 3_2:+79580382 None:intergenic
TTTGAAGTTTCTCCTGTTAC+AGG 0.213794 3_2:-79580055 Msa0428630:three_prime_UTR
GACAACGGGAGCCTGAAATT+TGG 0.254950 3_2:+79582832 None:intergenic
TGTTGAGTAAGTCTTATTCT+AGG 0.272769 3_2:-79580362 Msa0428630:three_prime_UTR
TTCCAATAGAAAAGAGATAT+TGG 0.280187 3_2:-79580088 Msa0428630:three_prime_UTR
TTGACAAATATACTAATTCT+TGG 0.281522 3_2:-79579914 Msa0428630:three_prime_UTR
TCCAATAGAAAAGAGATATT+GGG 0.302551 3_2:-79580087 Msa0428630:three_prime_UTR
GTATATTAAATATTCCCTTC+TGG 0.303331 3_2:-79580257 Msa0428630:three_prime_UTR
GACTTGGCTTCTTTATTTAT+GGG 0.304417 3_2:-79580231 Msa0428630:three_prime_UTR
CGCTCAACTGGTTGATTTGA+AGG 0.309764 3_2:-79580413 Msa0428630:three_prime_UTR
ACCGCTCGCGTCCAAATTTC+AGG 0.313707 3_2:-79582843 Msa0428630:CDS
AAATCCATTTCTTCTCTTGC+GGG 0.335431 3_2:-79582800 Msa0428630:CDS
AACATCGATATGAGGAATTT+CGG 0.343643 3_2:+79582886 None:intergenic
GACTCAAGACTGTCAAAGTA+TGG 0.351651 3_2:+79580689 None:intergenic
TATATTAAATATTCCCTTCT+GGG 0.374453 3_2:-79580256 Msa0428630:three_prime_UTR
TGGATGATCAAGTGCTGTTT+TGG 0.381295 3_2:+79580709 None:intergenic
GGATCGTTATCGCTCCTTCC+CGG 0.382171 3_2:-79582726 Msa0428630:CDS
CAATCTGTTTCAGCTTGAAT+AGG 0.382751 3_2:+79582351 None:intergenic
TATAGCCACGAGCCTGTAAC+AGG 0.392712 3_2:+79580043 None:intergenic
TCTCTACTTATTCGCTCAAC+TGG 0.410693 3_2:-79580425 Msa0428630:three_prime_UTR
CGAATAAGTAGAGAATATTC+TGG 0.415773 3_2:+79580434 None:intergenic
TTTCTTCTCTTGCGGGTCCA+TGG 0.418919 3_2:-79582793 Msa0428630:CDS
AATTTGGACGCGAGCGGTGG+TGG 0.421068 3_2:+79582848 None:intergenic
TTGCAAAACTTCTTCATGAC+TGG 0.424344 3_2:-79582305 Msa0428630:CDS
GAAATCCATTTCTTCTCTTG+CGG 0.431932 3_2:-79582801 Msa0428630:CDS
CAACTATCTAAATCCATGAT+AGG 0.435106 3_2:+79580516 None:intergenic
TGGACCCGCAAGAGAAGAAA+TGG 0.439446 3_2:+79582796 None:intergenic
TTGGCATATATTCTCTCAGC+AGG 0.451017 3_2:+79580728 None:intergenic
AATAGAACAGTAGTGACTAT+AGG 0.453983 3_2:+79579981 None:intergenic
ATAGTTGTCAAAAGAATATG+AGG 0.472384 3_2:-79580500 Msa0428630:three_prime_UTR
TGGACCTGTGGAAATACTAT+AGG 0.472845 3_2:-79580476 Msa0428630:three_prime_UTR
TTGGTAATTTGGGGTTTAGA+AGG 0.473415 3_2:-79580164 Msa0428630:three_prime_UTR
GATTTCACGAAAACGACAAC+GGG 0.478240 3_2:+79582818 None:intergenic
CTATTCAAGCTGAAACAGAT+TGG 0.478401 3_2:-79582350 Msa0428630:CDS
TTCGGCTGCTTGGTAATTTG+GGG 0.483603 3_2:-79580173 Msa0428630:three_prime_UTR
AAATGTTGGCTTCGGCTGCT+TGG 0.485908 3_2:-79580183 Msa0428630:three_prime_UTR
TATGTGAAAATAGCCTATCA+TGG 0.496512 3_2:-79580529 Msa0428630:three_prime_UTR
ACAAATCTCAATATTAATGA+TGG 0.498885 3_2:-79580662 Msa0428630:CDS
TATTCAAGCTGAAACAGATT+GGG 0.504544 3_2:-79582349 Msa0428630:CDS
GTTTGAAATCAAATATTCCA+TGG 0.505698 3_2:+79579869 None:intergenic
TTTAGAAGGTCTATATGCCT+TGG 0.506552 3_2:-79580150 Msa0428630:three_prime_UTR
AACAACACTGTCATAAATGT+TGG 0.506637 3_2:-79580197 Msa0428630:three_prime_UTR
AGAAAAGAGATATTGGGGAT+TGG 0.507927 3_2:-79580081 Msa0428630:three_prime_UTR
TATTCCCTTCTGGGCAGACT+TGG 0.517816 3_2:-79580247 Msa0428630:three_prime_UTR
ACTGTCATAAATGTTGGCTT+CGG 0.521944 3_2:-79580191 Msa0428630:three_prime_UTR
TCTGTCGTTATCAAAATCCA+TGG 0.523019 3_2:-79579886 Msa0428630:three_prime_UTR
ATGGCTCTAGCCAAAATGTT+TGG 0.526572 3_2:-79580643 Msa0428630:CDS
CCAATAGAAAAGAGATATTG+GGG 0.529315 3_2:-79580086 Msa0428630:three_prime_UTR
CTTGCGAAAACATCGATATG+AGG 0.531413 3_2:+79582878 None:intergenic
TGGTGCAGAAGTGTCTGTTA+AGG 0.538845 3_2:-79580623 Msa0428630:exon
AGAAGCCAAGTCTGCCCAGA+AGG 0.544881 3_2:+79580242 None:intergenic
CACCACGACTGTAAAGTGTA+AGG 0.546440 3_2:+79582748 None:intergenic
ATTTGGACGCGAGCGGTGGT+GGG 0.548219 3_2:+79582849 None:intergenic
AGAATTAGCATTTAACTCCA+AGG 0.557775 3_2:+79580133 None:intergenic
TTTGGACGCGAGCGGTGGTG+GGG 0.559838 3_2:+79582850 None:intergenic
CTTCTCTTGCGGGTCCATGG+AGG 0.564143 3_2:-79582790 Msa0428630:CDS
ATCCTTACACTTTACAGTCG+TGG 0.566131 3_2:-79582750 Msa0428630:CDS
AGAATATGAGGTTGGACCTG+TGG 0.568102 3_2:-79580488 Msa0428630:three_prime_UTR
TGAAATTTGGACGCGAGCGG+TGG 0.569521 3_2:+79582845 None:intergenic
AAAATTATACTGTTTGTCAG+AGG 0.572025 3_2:-79582702 Msa0428630:CDS
CTGTTTATAAACTGCTGCCA+CGG 0.577431 3_2:-79580112 Msa0428630:three_prime_UTR
TGTTTCAGCTTGAATAGGAG+AGG 0.589502 3_2:+79582356 None:intergenic
CATTGTATTGCAGTCAGTGA+AGG 0.592085 3_2:-79580597 Msa0428630:three_prime_UTR
AAATTATACTGTTTGTCAGA+GGG 0.592529 3_2:-79582701 Msa0428630:CDS
TTTCTCCTGTTACAGGCTCG+TGG 0.593798 3_2:-79580048 Msa0428630:three_prime_UTR
GTTTCAGCTTGAATAGGAGA+GGG 0.593887 3_2:+79582357 None:intergenic
GTGAAGGTAAATTCACTACA+TGG 0.596530 3_2:-79580581 Msa0428630:three_prime_UTR
GGATTTCACGAAAACGACAA+CGG 0.596944 3_2:+79582817 None:intergenic
GCATCCTATAGTATTTCCAC+AGG 0.604758 3_2:+79580472 None:intergenic
TTGTCAAAAGAATATGAGGT+TGG 0.611042 3_2:-79580496 Msa0428630:three_prime_UTR
CAAGCTGAAACAGATTGGGT+AGG 0.611478 3_2:-79582345 Msa0428630:CDS
GCCTGAAATTTGGACGCGAG+CGG 0.616835 3_2:+79582842 None:intergenic
GAAGCCAAGTCTGCCCAGAA+GGG 0.634146 3_2:+79580243 None:intergenic
ACTGGCAACAAACACAAACA+AGG 0.659426 3_2:-79582287 Msa0428630:CDS
CTTACACTTTACAGTCGTGG+TGG 0.668224 3_2:-79582747 Msa0428630:CDS
GGCAACAAACACAAACAAGG+AGG 0.748838 3_2:-79582284 Msa0428630:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTCATGAATAAAAAAA+CGG + chr3_2:79580860-79580879 None:intergenic 10.0%
!!! TGATTAAAATGTAATTTTTA+TGG + chr3_2:79581685-79581704 None:intergenic 10.0%
!!! ATAAAAATTACATTTTAATC+AGG - chr3_2:79581684-79581703 Msa0428630:intron 10.0%
!!! ATATGATTTTGTTAATTCAA+AGG - chr3_2:79580558-79580577 Msa0428630:three_prime_UTR 15.0%
!!! TCTTCAATAATTTTTTTGAA+AGG - chr3_2:79582480-79582499 Msa0428630:intron 15.0%
!!! TTGTTTTTATCTACTTTTGT+AGG - chr3_2:79580377-79580396 Msa0428630:three_prime_UTR 20.0%
!!! ATTCTTTTTTCTTTGCTTAT+CGG - chr3_2:79580970-79580989 Msa0428630:intron 20.0%
!!! TCAACATTGATATATTTTCT+TGG - chr3_2:79581262-79581281 Msa0428630:intron 20.0%
!!! TAATAAAAGCATATAGTCAT+TGG + chr3_2:79581585-79581604 None:intergenic 20.0%
!! ACAAATCTCAATATTAATGA+TGG - chr3_2:79582116-79582135 Msa0428630:intron 20.0%
!!! ATTTGTTTTACTCTTTTAGA+GGG - chr3_2:79582451-79582470 Msa0428630:intron 20.0%
!! TATATTAAATATTCCCTTCT+GGG - chr3_2:79582522-79582541 Msa0428630:intron 20.0%
!! TTGACAAATATACTAATTCT+TGG - chr3_2:79582864-79582883 Msa0428630:CDS 20.0%
!! ACAAACAGTATAATTTTACC+GGG + chr3_2:79580073-79580092 None:intergenic 25.0%
!! GACAAACAGTATAATTTTAC+CGG + chr3_2:79580074-79580093 None:intergenic 25.0%
! AAAATTATACTGTTTGTCAG+AGG - chr3_2:79580076-79580095 Msa0428630:three_prime_UTR 25.0%
! AAATTATACTGTTTGTCAGA+GGG - chr3_2:79580077-79580096 Msa0428630:three_prime_UTR 25.0%
! AAGGAACACAATTATTCAAT+TGG + chr3_2:79580164-79580183 None:intergenic 25.0%
! ACTTCCAGATTAAATAAACA+GGG + chr3_2:79580189-79580208 None:intergenic 25.0%
! TACTTCCAGATTAAATAAAC+AGG + chr3_2:79580190-79580209 None:intergenic 25.0%
!! GAAGTTTTGCAAATTCTTTT+TGG + chr3_2:79580465-79580484 None:intergenic 25.0%
! CATATTCTGCAATTGATTAA+GGG + chr3_2:79580543-79580562 None:intergenic 25.0%
! TCATATTCTGCAATTGATTA+AGG + chr3_2:79580544-79580563 None:intergenic 25.0%
! CACAAAACATCAGAAAAAAA+TGG + chr3_2:79580593-79580612 None:intergenic 25.0%
! CAAAAATGCAAAATTTGAAG+AGG + chr3_2:79580636-79580655 None:intergenic 25.0%
! AGGTAATTATAATGTTGATG+AGG + chr3_2:79580823-79580842 None:intergenic 25.0%
! TCATCAACATTATAATTACC+TGG - chr3_2:79580822-79580841 Msa0428630:intron 25.0%
! TCATGAATAAAAAAACGGAA+TGG + chr3_2:79580855-79580874 None:intergenic 25.0%
! ATTAAATTGTGATCCTGAAA+TGG - chr3_2:79581071-79581090 Msa0428630:intron 25.0%
!! ACTTATATGTTGTCATTTAG+TGG - chr3_2:79581170-79581189 Msa0428630:intron 25.0%
! TCATCATCTATATTCAATTC+AGG - chr3_2:79581204-79581223 Msa0428630:intron 25.0%
! GGAGTATGAATATTAGAAAT+TGG + chr3_2:79581290-79581309 None:intergenic 25.0%
! AGTGAAATAAAAGATGTAAG+AGG + chr3_2:79581311-79581330 None:intergenic 25.0%
! ATAGTGAATAACATGCATTA+AGG + chr3_2:79581436-79581455 None:intergenic 25.0%
!!! CATTTTAATCAGGTGATATA+TGG - chr3_2:79581694-79581713 Msa0428630:intron 25.0%
! ATAGTTGTCAAAAGAATATG+AGG - chr3_2:79582278-79582297 Msa0428630:intron 25.0%
!!! CATTTGTTTTACTCTTTTAG+AGG - chr3_2:79582450-79582469 Msa0428630:intron 25.0%
! GTATATTAAATATTCCCTTC+TGG - chr3_2:79582521-79582540 Msa0428630:intron 25.0%
! TTCCAATAGAAAAGAGATAT+TGG - chr3_2:79582690-79582709 Msa0428630:CDS 25.0%
! TCCAATAGAAAAGAGATATT+GGG - chr3_2:79582691-79582710 Msa0428630:CDS 25.0%
!! GTTTGAAATCAAATATTCCA+TGG + chr3_2:79582912-79582931 None:intergenic 25.0%
AACATCGATATGAGGAATTT+CGG + chr3_2:79579895-79579914 None:intergenic 30.0%
TTCCAGATTAAATAAACAGG+GGG + chr3_2:79580187-79580206 None:intergenic 30.0%
CTTCCAGATTAAATAAACAG+GGG + chr3_2:79580188-79580207 None:intergenic 30.0%
ACAGAAAACTAAAATGCAGA+GGG + chr3_2:79580279-79580298 None:intergenic 30.0%
AACAGAAAACTAAAATGCAG+AGG + chr3_2:79580280-79580299 None:intergenic 30.0%
! TAGTTTTCTGTTTGTGAACT+TGG - chr3_2:79580288-79580307 Msa0428630:three_prime_UTR 30.0%
TATTCAAGCTGAAACAGATT+GGG - chr3_2:79580429-79580448 Msa0428630:three_prime_UTR 30.0%
ATGTATGCAGGATATGAATT+GGG + chr3_2:79580926-79580945 None:intergenic 30.0%
TATGTATGCAGGATATGAAT+TGG + chr3_2:79580927-79580946 None:intergenic 30.0%
ATTTACCAATCTATGTATGC+AGG + chr3_2:79580938-79580957 None:intergenic 30.0%
TCGGATTTATTTGAAAAGAG+AGG - chr3_2:79580989-79581008 Msa0428630:intron 30.0%
CGGATTTATTTGAAAAGAGA+GGG - chr3_2:79580990-79581009 Msa0428630:intron 30.0%
! CTTGCATGTTTGTTGAAATT+AGG + chr3_2:79581025-79581044 None:intergenic 30.0%
TCAAAACTGTTACACACAAT+AGG - chr3_2:79581127-79581146 Msa0428630:intron 30.0%
ACATATAAGTTTGAGTCTTG+AGG + chr3_2:79581160-79581179 None:intergenic 30.0%
ATGAAGATAGAATAGATGGA+AGG + chr3_2:79581461-79581480 None:intergenic 30.0%
TGACATGAAGATAGAATAGA+TGG + chr3_2:79581465-79581484 None:intergenic 30.0%
!!! GATTTTTTCTCCCTAACTTT+TGG - chr3_2:79581495-79581514 Msa0428630:intron 30.0%
TGTGAAATCTCCAAAAGTTA+GGG + chr3_2:79581508-79581527 None:intergenic 30.0%
ATGTGAAATCTCCAAAAGTT+AGG + chr3_2:79581509-79581528 None:intergenic 30.0%
GACAACACACGTATATAAAA+AGG + chr3_2:79581546-79581565 None:intergenic 30.0%
! ATTATTGCTGTTATTTGTGC+AGG - chr3_2:79581601-79581620 Msa0428630:intron 30.0%
! TTATTGCTGTTATTTGTGCA+GGG - chr3_2:79581602-79581621 Msa0428630:intron 30.0%
ATCGTAGTATATCTTAGTCT+TGG - chr3_2:79581822-79581841 Msa0428630:intron 30.0%
TAGTGATAGGAAATTATGAC+TGG - chr3_2:79581874-79581893 Msa0428630:intron 30.0%
!! AGGCTATTTTCACATACTTA+TGG + chr3_2:79582245-79582264 None:intergenic 30.0%
TATGTGAAAATAGCCTATCA+TGG - chr3_2:79582249-79582268 Msa0428630:intron 30.0%
CAACTATCTAAATCCATGAT+AGG + chr3_2:79582265-79582284 None:intergenic 30.0%
TTGTCAAAAGAATATGAGGT+TGG - chr3_2:79582282-79582301 Msa0428630:intron 30.0%
CGAATAAGTAGAGAATATTC+TGG + chr3_2:79582347-79582366 None:intergenic 30.0%
ACAACTTCTCAAATCTTTAC+AGG + chr3_2:79582399-79582418 None:intergenic 30.0%
!! TGTTGAGTAAGTCTTATTCT+AGG - chr3_2:79582416-79582435 Msa0428630:intron 30.0%
! AGACTTGGCTTCTTTATTTA+TGG - chr3_2:79582546-79582565 Msa0428630:intron 30.0%
! GACTTGGCTTCTTTATTTAT+GGG - chr3_2:79582547-79582566 Msa0428630:intron 30.0%
AACAACACTGTCATAAATGT+TGG - chr3_2:79582581-79582600 Msa0428630:intron 30.0%
AGAATTAGCATTTAACTCCA+AGG + chr3_2:79582648-79582667 None:intergenic 30.0%
CCAATAGAAAAGAGATATTG+GGG - chr3_2:79582692-79582711 Msa0428630:CDS 30.0%
AATAGAACAGTAGTGACTAT+AGG + chr3_2:79582800-79582819 None:intergenic 30.0%
! TCATATCGATGTTTTCGCAA+GGG - chr3_2:79579902-79579921 Msa0428630:three_prime_UTR 35.0%
GAAATCCATTTCTTCTCTTG+CGG - chr3_2:79579977-79579996 Msa0428630:three_prime_UTR 35.0%
AAATCCATTTCTTCTCTTGC+GGG - chr3_2:79579978-79579997 Msa0428630:three_prime_UTR 35.0%
! ACAGTATAATTTTACCGGGA+AGG + chr3_2:79580069-79580088 None:intergenic 35.0%
! CTGCATTATTTCTTGCTTGT+GGG - chr3_2:79580134-79580153 Msa0428630:three_prime_UTR 35.0%
! TGCATTATTTCTTGCTTGTG+GGG - chr3_2:79580135-79580154 Msa0428630:three_prime_UTR 35.0%
AGATTAAATAAACAGGGGGA+AGG + chr3_2:79580183-79580202 None:intergenic 35.0%
TTCCCCCTGTTTATTTAATC+TGG - chr3_2:79580182-79580201 Msa0428630:three_prime_UTR 35.0%
AATCGCTTGATTCTTTGTTG+TGG - chr3_2:79580317-79580336 Msa0428630:three_prime_UTR 35.0%
! ATCGCTTGATTCTTTGTTGT+GGG - chr3_2:79580318-79580337 Msa0428630:three_prime_UTR 35.0%
! TTGATTCTTTGTTGTGGGTA+TGG - chr3_2:79580323-79580342 Msa0428630:three_prime_UTR 35.0%
CAATCTGTTTCAGCTTGAAT+AGG + chr3_2:79580430-79580449 None:intergenic 35.0%
CTATTCAAGCTGAAACAGAT+TGG - chr3_2:79580428-79580447 Msa0428630:three_prime_UTR 35.0%
TTGCAAAACTTCTTCATGAC+TGG - chr3_2:79580473-79580492 Msa0428630:three_prime_UTR 35.0%
TTAAGGGAATGAGACAAAGA+CGG + chr3_2:79580527-79580546 None:intergenic 35.0%
CATATCCTGCATACATAGAT+TGG - chr3_2:79580930-79580949 Msa0428630:intron 35.0%
! ATATGTTGTCATTTAGTGGC+AGG - chr3_2:79581174-79581193 Msa0428630:intron 35.0%
! TATGTTGTCATTTAGTGGCA+GGG - chr3_2:79581175-79581194 Msa0428630:intron 35.0%
TTCAGTGCATCATTAGACAA+AGG - chr3_2:79581349-79581368 Msa0428630:intron 35.0%
GCATCATTAGACAAAGGTAA+AGG - chr3_2:79581355-79581374 Msa0428630:intron 35.0%
!! GCAAGTATCTTGGTTCTAAT+GGG + chr3_2:79581414-79581433 None:intergenic 35.0%
ATGCATTAAGGCAAGTATCT+TGG + chr3_2:79581424-79581443 None:intergenic 35.0%
AGATTTCACATTGCTCTGAT+TGG - chr3_2:79581518-79581537 Msa0428630:intron 35.0%
TGTGTTGTCAACTTTGATCA+TGG - chr3_2:79581557-79581576 Msa0428630:intron 35.0%
TGGTGATAGATGTCAGAATT+TGG - chr3_2:79581659-79581678 Msa0428630:intron 35.0%
AGCAAGGAAAAGTAACATTG+AGG + chr3_2:79581761-79581780 None:intergenic 35.0%
! GGTTACTTTCATGTAGTGAT+AGG - chr3_2:79581861-79581880 Msa0428630:intron 35.0%
ATTGAACATGGTGAAAGTGA+AGG + chr3_2:79581945-79581964 None:intergenic 35.0%
GAGACACACATTATTGAACA+TGG + chr3_2:79581957-79581976 None:intergenic 35.0%
ATCCAATTACAACCACAGTT+CGG + chr3_2:79581994-79582013 None:intergenic 35.0%
GTGAAGGTAAATTCACTACA+TGG - chr3_2:79582197-79582216 Msa0428630:intron 35.0%
!! ACTGTCATAAATGTTGGCTT+CGG - chr3_2:79582587-79582606 Msa0428630:intron 35.0%
! TTGGTAATTTGGGGTTTAGA+AGG - chr3_2:79582614-79582633 Msa0428630:intron 35.0%
TTTAGAAGGTCTATATGCCT+TGG - chr3_2:79582628-79582647 Msa0428630:intron 35.0%
! CCCCAATATCTCTTTTCTAT+TGG + chr3_2:79582695-79582714 None:intergenic 35.0%
AGAAAAGAGATATTGGGGAT+TGG - chr3_2:79582697-79582716 Msa0428630:CDS 35.0%
! TTTGAAGTTTCTCCTGTTAC+AGG - chr3_2:79582723-79582742 Msa0428630:CDS 35.0%
TCTGTCGTTATCAAAATCCA+TGG - chr3_2:79582892-79582911 Msa0428630:CDS 35.0%
CTTGCGAAAACATCGATATG+AGG + chr3_2:79579903-79579922 None:intergenic 40.0%
! CTCATATCGATGTTTTCGCA+AGG - chr3_2:79579901-79579920 Msa0428630:three_prime_UTR 40.0%
GATTTCACGAAAACGACAAC+GGG + chr3_2:79579963-79579982 None:intergenic 40.0%
GGATTTCACGAAAACGACAA+CGG + chr3_2:79579964-79579983 None:intergenic 40.0%
ATCCTTACACTTTACAGTCG+TGG - chr3_2:79580028-79580047 Msa0428630:three_prime_UTR 40.0%
! GCTGCATTATTTCTTGCTTG+TGG - chr3_2:79580133-79580152 Msa0428630:three_prime_UTR 40.0%
GTTTCAGCTTGAATAGGAGA+GGG + chr3_2:79580424-79580443 None:intergenic 40.0%
TGTTTCAGCTTGAATAGGAG+AGG + chr3_2:79580425-79580444 None:intergenic 40.0%
ACTGGCAACAAACACAAACA+AGG - chr3_2:79580491-79580510 Msa0428630:three_prime_UTR 40.0%
CAGGATATGAATTGGGCTTA+TGG + chr3_2:79580919-79580938 None:intergenic 40.0%
!! GGCAAGTATCTTGGTTCTAA+TGG + chr3_2:79581415-79581434 None:intergenic 40.0%
! GTAGAAAGCATACAGTCAAG+TGG - chr3_2:79581639-79581658 Msa0428630:intron 40.0%
! GAACTGACACTGATTAAGCA+AGG + chr3_2:79581777-79581796 None:intergenic 40.0%
! CTTGGTAGTTATCTGAACTG+AGG - chr3_2:79581840-79581859 Msa0428630:intron 40.0%
! TGTACTTGAGCATTTTCTGC+AGG + chr3_2:79582028-79582047 None:intergenic 40.0%
TTGGCATATATTCTCTCAGC+AGG + chr3_2:79582053-79582072 None:intergenic 40.0%
!!! TGGATGATCAAGTGCTGTTT+TGG + chr3_2:79582072-79582091 None:intergenic 40.0%
GACTCAAGACTGTCAAAGTA+TGG + chr3_2:79582092-79582111 None:intergenic 40.0%
!! ATGGCTCTAGCCAAAATGTT+TGG - chr3_2:79582135-79582154 Msa0428630:intron 40.0%
! CACTTCTGCACCAAACATTT+TGG + chr3_2:79582148-79582167 None:intergenic 40.0%
CATTGTATTGCAGTCAGTGA+AGG - chr3_2:79582181-79582200 Msa0428630:intron 40.0%
TGGACCTGTGGAAATACTAT+AGG - chr3_2:79582302-79582321 Msa0428630:CDS 40.0%
GCATCCTATAGTATTTCCAC+AGG + chr3_2:79582309-79582328 None:intergenic 40.0%
TCTCTACTTATTCGCTCAAC+TGG - chr3_2:79582353-79582372 Msa0428630:CDS 40.0%
CTGTTTATAAACTGCTGCCA+CGG - chr3_2:79582666-79582685 Msa0428630:intron 40.0%
! CTCTTTTCTATTGGAAACCG+TGG + chr3_2:79582686-79582705 None:intergenic 40.0%
! TCCATGGAGGTTTTTGTGTC+AGG - chr3_2:79580001-79580020 Msa0428630:three_prime_UTR 45.0%
! CCATGGAGGTTTTTGTGTCA+GGG - chr3_2:79580002-79580021 Msa0428630:three_prime_UTR 45.0%
CACCACGACTGTAAAGTGTA+AGG + chr3_2:79580033-79580052 None:intergenic 45.0%
CTTACACTTTACAGTCGTGG+TGG - chr3_2:79580031-79580050 Msa0428630:three_prime_UTR 45.0%
CTGGAAGTAGCAGTTATGTG+TGG - chr3_2:79580201-79580220 Msa0428630:three_prime_UTR 45.0%
CAAGCTGAAACAGATTGGGT+AGG - chr3_2:79580433-79580452 Msa0428630:three_prime_UTR 45.0%
GGCAACAAACACAAACAAGG+AGG - chr3_2:79580494-79580513 Msa0428630:three_prime_UTR 45.0%
AAACGGAATGGCATCAAACC+AGG + chr3_2:79580843-79580862 None:intergenic 45.0%
ATTGTGATCCTGAAATGGCG+TGG - chr3_2:79581076-79581095 Msa0428630:intron 45.0%
ATACAGTTCCACGCCATTTC+AGG + chr3_2:79581087-79581106 None:intergenic 45.0%
AAATGGCGTGGAACTGTATG+TGG - chr3_2:79581088-79581107 Msa0428630:intron 45.0%
AATGGCGTGGAACTGTATGT+GGG - chr3_2:79581089-79581108 Msa0428630:intron 45.0%
TGCCTAGAAACCATAGTGCT+AGG - chr3_2:79581227-79581246 Msa0428630:intron 45.0%
CACCTAGCACTATGGTTTCT+AGG + chr3_2:79581232-79581251 None:intergenic 45.0%
AAACCATAGTGCTAGGTGAC+AGG - chr3_2:79581234-79581253 Msa0428630:intron 45.0%
AAACCTGTCACCTAGCACTA+TGG + chr3_2:79581240-79581259 None:intergenic 45.0%
GAACATGGTGAAAGTGAAGG+TGG + chr3_2:79581942-79581961 None:intergenic 45.0%
! ATTTTCTTTCCGCCGAACTG+TGG - chr3_2:79581979-79581998 Msa0428630:intron 45.0%
CAATTACAACCACAGTTCGG+CGG + chr3_2:79581991-79582010 None:intergenic 45.0%
!! TGGTGCAGAAGTGTCTGTTA+AGG - chr3_2:79582155-79582174 Msa0428630:intron 45.0%
AGAATATGAGGTTGGACCTG+TGG - chr3_2:79582290-79582309 Msa0428630:CDS 45.0%
!! CGCTCAACTGGTTGATTTGA+AGG - chr3_2:79582365-79582384 Msa0428630:CDS 45.0%
! CTTCGGCTGCTTGGTAATTT+GGG - chr3_2:79582604-79582623 Msa0428630:intron 45.0%
! TTCGGCTGCTTGGTAATTTG+GGG - chr3_2:79582605-79582624 Msa0428630:intron 45.0%
GACAACGGGAGCCTGAAATT+TGG + chr3_2:79579949-79579968 None:intergenic 50.0%
TGGACCCGCAAGAGAAGAAA+TGG + chr3_2:79579985-79580004 None:intergenic 50.0%
TTTCTTCTCTTGCGGGTCCA+TGG - chr3_2:79579985-79580004 Msa0428630:three_prime_UTR 50.0%
CCCTGACACAAAAACCTCCA+TGG + chr3_2:79580005-79580024 None:intergenic 50.0%
CGATGGAGCAAACACACACA+AGG + chr3_2:79580250-79580269 None:intergenic 50.0%
AATGCAGAGGGATGAGACGA+TGG + chr3_2:79580267-79580286 None:intergenic 50.0%
! GTGGAACTGTATGTGGGCTA+TGG - chr3_2:79581095-79581114 Msa0428630:intron 50.0%
! GTATGTGGGCTATGGTAGTC+TGG - chr3_2:79581103-79581122 Msa0428630:intron 50.0%
CGCCGAACTGTGGTTGTAAT+TGG - chr3_2:79581989-79582008 Msa0428630:intron 50.0%
TATTCCCTTCTGGGCAGACT+TGG - chr3_2:79582531-79582550 Msa0428630:intron 50.0%
!! AAATGTTGGCTTCGGCTGCT+TGG - chr3_2:79582595-79582614 Msa0428630:intron 50.0%
! GCTTCGGCTGCTTGGTAATT+TGG - chr3_2:79582603-79582622 Msa0428630:intron 50.0%
TTTCTCCTGTTACAGGCTCG+TGG - chr3_2:79582730-79582749 Msa0428630:CDS 50.0%
TATAGCCACGAGCCTGTAAC+AGG + chr3_2:79582738-79582757 None:intergenic 50.0%
TGAAATTTGGACGCGAGCGG+TGG + chr3_2:79579936-79579955 None:intergenic 55.0%
ACCGCTCGCGTCCAAATTTC+AGG - chr3_2:79579935-79579954 Msa0428630:three_prime_UTR 55.0%
GCCTGAAATTTGGACGCGAG+CGG + chr3_2:79579939-79579958 None:intergenic 55.0%
GGATCGTTATCGCTCCTTCC+CGG - chr3_2:79580052-79580071 Msa0428630:three_prime_UTR 55.0%
GAAGCCAAGTCTGCCCAGAA+GGG + chr3_2:79582538-79582557 None:intergenic 55.0%
AGAAGCCAAGTCTGCCCAGA+AGG + chr3_2:79582539-79582558 None:intergenic 55.0%
ATTTGGACGCGAGCGGTGGT+GGG + chr3_2:79579932-79579951 None:intergenic 60.0%
AATTTGGACGCGAGCGGTGG+TGG + chr3_2:79579933-79579952 None:intergenic 60.0%
CTTCTCTTGCGGGTCCATGG+AGG - chr3_2:79579988-79580007 Msa0428630:three_prime_UTR 60.0%
! CAGAGGGTCGACGCTTTTCG+TGG - chr3_2:79580093-79580112 Msa0428630:three_prime_UTR 60.0%
! AGGGTCGACGCTTTTCGTGG+CGG - chr3_2:79580096-79580115 Msa0428630:three_prime_UTR 60.0%
TTTGGACGCGAGCGGTGGTG+GGG + chr3_2:79579931-79579950 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 79579869 79582931 79579869 ID=Msa0428630;Name=Msa0428630
chr3_2 mRNA 79579869 79582931 79579869 ID=Msa0428630-mRNA-1;Parent=Msa0428630;Name=Msa0428630-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0.33|0.5|1|1|1|4|756|173
chr3_2 exon 79579869 79580768 79579869 ID=Msa0428630-mRNA-1:exon:21493;Parent=Msa0428630-mRNA-1
chr3_2 exon 79581171 79581177 79581171 ID=Msa0428630-mRNA-1:exon:21492;Parent=Msa0428630-mRNA-1
chr3_2 exon 79582285 79582401 79582285 ID=Msa0428630-mRNA-1:exon:21491;Parent=Msa0428630-mRNA-1
chr3_2 exon 79582678 79582931 79582678 ID=Msa0428630-mRNA-1:exon:21490;Parent=Msa0428630-mRNA-1
chr3_2 CDS 79582678 79582931 79582678 ID=Msa0428630-mRNA-1:cds;Parent=Msa0428630-mRNA-1
chr3_2 CDS 79582285 79582401 79582285 ID=Msa0428630-mRNA-1:cds;Parent=Msa0428630-mRNA-1
chr3_2 CDS 79581171 79581177 79581171 ID=Msa0428630-mRNA-1:cds;Parent=Msa0428630-mRNA-1
chr3_2 CDS 79580625 79580768 79580625 ID=Msa0428630-mRNA-1:cds;Parent=Msa0428630-mRNA-1
chr3_2 three_prime_UTR 79579869 79580624 79579869 ID=Msa0428630-mRNA-1:three_prime_utr;Parent=Msa0428630-mRNA-1
Gene Sequence

>Msa0428630

ATGATGTTTCGAATTAGTTTGAACCGAAATTCCTCATATCGATGTTTTCGCAAGGGTTTCCCCACCACCGCTCGCGTCCAAATTTCAGGCTCCCGTTGTCGTTTTCGTGAAATCCATTTCTTCTCTTGCGGGTCCATGGAGGTTTTTGTGTCAGGGTACATCCTTACACTTTACAGTCGTGGTGGATCGTTATCGCTCCTTCCCGGTAAAATTATACTGTTTGTCAGAGGGTCGACGCTTTTCGTGGCGGATCAGAAATCTGATTTGTTTGTGTTTCCCTCTCCTATTCAAGCTGAAACAGATTGGGTAGGAAAGTTCCAAAAAGAATTTGCAAAACTTCTTCATGACTGGCAACAAACACAAACAAGGAGGGTGTGTAAAATGCTCAAGTACAATCCTGCTGAGAGAATATATGCCAAAACAGCACTTGATCATCCATACTTTGACAGTCTTGAGTCATTGACAAATCTCAATATTAATGATGGCTCTAGCCAAAATGTTTGGTGCAGAAGTGTCTGTTAA
Protein sequence

>Msa0428630

MMFRISLNRNSSYRCFRKGFPTTARVQISGSRCRFREIHFFSCGSMEVFVSGYILTLYSRGGSLSLLPGKIILFVRGSTLFVADQKSDLFVFPSPIQAETDWVGKFQKEFAKLLHDWQQTQTRRVCKMLKYNPAERIYAKTALDHPYFDSLESLTNLNINDGSSQNVWCRSVC*