AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0431890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431890 MtrunA17_Chr3g0136701 97.586 290 7 0 1 290 1 290 0.0 590
Msa0431890 MtrunA17_Chr1g0150471 54.403 318 115 7 1 290 1 316 3.82e-106 311
Msa0431890 MtrunA17_Chr4g0072871 51.205 332 101 11 4 280 20 345 4.65e-89 270
Msa0431890 MtrunA17_Chr7g0220721 63.043 92 34 0 3 94 23 114 1.31e-37 137
Msa0431890 MtrunA17_Chr7g0220721 71.429 70 9 2 231 289 326 395 4.99e-23 97.4
Msa0431890 MtrunA17_Chr5g0405151 50.602 83 33 1 5 79 4 86 1.06e-20 87.4
Msa0431890 MtrunA17_Chr5g0429341 43.333 90 51 0 5 94 4 93 2.51e-19 87.4
Msa0431890 MtrunA17_Chr1g0155811 43.299 97 45 1 6 92 5 101 4.99e-19 84.7
Msa0431890 MtrunA17_Chr2g0314671 44.792 96 44 1 6 92 5 100 1.08e-18 84.0
Msa0431890 MtrunA17_Chr4g0033641 43.750 96 45 1 6 92 5 100 1.10e-18 83.2
Msa0431890 MtrunA17_Chr2g0329511 42.708 96 46 1 6 92 5 100 1.56e-17 80.1
Msa0431890 MtrunA17_Chr4g0035651 39.175 97 49 1 6 92 5 101 5.64e-17 78.2
Msa0431890 MtrunA17_Chr3g0145061 38.144 97 50 1 6 92 5 101 6.44e-17 78.2
Msa0431890 MtrunA17_Chr7g0268691 39.326 89 53 1 6 94 20 107 3.01e-16 78.2
Msa0431890 MtrunA17_Chr3g0120711 39.216 102 61 1 5 106 4 104 3.82e-16 78.2
Msa0431890 MtrunA17_Chr3g0120711 44.872 78 40 1 202 279 327 401 3.16e-11 63.5
Msa0431890 MtrunA17_Chr2g0322941 45.122 82 45 0 5 86 43 124 3.12e-15 73.6
Msa0431890 MtrunA17_Chr3g0142221 37.895 95 48 1 6 89 5 99 8.82e-15 72.0
Msa0431890 MtrunA17_Chr7g0251781 44.444 72 40 0 6 77 5 76 1.33e-13 70.5
Msa0431890 MtrunA17_Chr2g0322951 38.318 107 46 2 189 279 37 139 1.25e-12 64.3
Msa0431890 MtrunA17_Chr1g0209071 39.189 74 45 0 4 77 3 76 1.53e-12 67.0
Msa0431890 MtrunA17_Chr1g0209861 61.818 55 17 1 224 274 370 424 3.06e-12 66.6
Msa0431890 MtrunA17_Chr8g0390971 70.455 44 13 0 232 275 358 401 4.87e-12 65.9
Msa0431890 MtrunA17_Chr5g0431351 66.667 45 15 0 230 274 172 216 7.14e-12 63.5
Msa0431890 MtrunA17_Chr3g0127941 49.231 65 32 1 219 283 617 680 7.36e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431890 AT5G24930.1 52.000 375 80 13 1 289 1 361 6.65e-95 285
Msa0431890 AT2G24790.1 54.266 293 95 11 2 277 4 274 4.73e-94 280
Msa0431890 AT5G57660.1 42.775 346 131 10 2 289 18 354 2.34e-74 232
Msa0431890 AT3G02380.1 40.351 342 141 9 3 289 13 346 4.42e-73 228
Msa0431890 AT5G15850.1 41.210 347 143 8 3 289 9 354 5.84e-70 220
Msa0431890 AT5G15840.1 39.335 361 134 14 6 289 20 372 1.29e-65 210
Msa0431890 AT2G24790.2 56.180 178 55 6 2 171 4 166 8.17e-58 185
Msa0431890 AT5G15840.2 41.935 186 82 7 6 166 20 204 2.12e-37 134
Msa0431890 AT1G49130.1 28.435 313 176 10 4 280 26 326 1.65e-22 95.5
Msa0431890 AT1G49130.2 28.435 313 176 10 4 280 19 319 1.85e-22 95.5
Msa0431890 AT4G38960.1 35.570 149 74 3 5 138 4 145 3.41e-20 86.3
Msa0431890 AT4G38960.6 35.570 149 74 3 5 138 4 145 3.41e-20 86.3
Msa0431890 AT2G21320.1 49.398 83 34 1 5 79 4 86 6.48e-20 85.1
Msa0431890 AT1G78600.2 42.017 119 56 3 6 111 5 123 4.97e-19 85.9
Msa0431890 AT1G78600.1 42.017 119 56 3 6 111 5 123 5.28e-19 85.5
Msa0431890 AT2G47890.3 42.857 91 50 1 4 94 11 99 4.59e-18 82.8
Msa0431890 AT2G47890.1 42.857 91 50 1 4 94 11 99 5.13e-18 83.2
Msa0431890 AT2G47890.1 27.273 308 161 14 3 276 53 331 1.35e-13 70.5
Msa0431890 AT2G47890.2 42.857 91 50 1 4 94 11 99 6.31e-18 81.3
Msa0431890 AT1G06040.1 43.750 96 45 1 6 92 5 100 7.36e-18 81.3
Msa0431890 AT1G06040.2 43.750 96 45 1 6 92 5 100 1.01e-17 79.3
Msa0431890 AT1G75540.1 40.404 99 47 1 6 92 5 103 1.17e-17 82.0
Msa0431890 AT2G33500.1 42.697 89 51 0 6 94 12 100 3.25e-17 81.6
Msa0431890 AT2G33500.1 52.632 57 27 0 220 276 345 401 1.09e-12 68.2
Msa0431890 AT2G33500.2 42.697 89 51 0 6 94 12 100 3.26e-17 81.6
Msa0431890 AT2G33500.2 52.632 57 27 0 220 276 344 400 1.12e-12 68.2
Msa0431890 AT2G31380.1 34.177 158 87 4 6 153 5 155 1.44e-16 77.8
Msa0431890 AT1G28050.1 41.573 89 52 0 6 94 9 97 4.62e-16 78.2
Msa0431890 AT1G28050.1 56.000 50 22 0 228 277 381 430 9.23e-11 62.4
Msa0431890 AT5G48250.1 38.043 92 55 1 5 94 4 95 1.11e-14 73.9
Msa0431890 AT3G07650.3 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
Msa0431890 AT3G07650.3 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
Msa0431890 AT3G07650.2 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
Msa0431890 AT3G07650.2 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
Msa0431890 AT3G07650.4 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
Msa0431890 AT3G07650.4 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
Msa0431890 AT3G07650.1 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
Msa0431890 AT3G07650.1 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
Msa0431890 AT4G10240.1 33.714 175 81 7 6 165 5 159 4.33e-14 68.9
Msa0431890 AT4G39070.1 37.755 98 51 1 5 92 4 101 1.71e-13 68.9
Msa0431890 AT1G73870.1 55.556 63 27 1 213 275 327 388 2.52e-13 70.1
Msa0431890 AT4G15250.1 40.476 84 50 0 6 89 5 88 4.70e-13 68.9
Msa0431890 AT1G68190.2 38.824 85 50 1 4 88 41 123 6.48e-13 68.9
Msa0431890 AT1G68190.1 38.824 85 50 1 4 88 12 94 7.72e-13 68.6
Msa0431890 AT1G68190.3 38.824 85 50 1 4 88 12 94 7.72e-13 68.6
Msa0431890 AT1G25440.1 36.029 136 72 5 158 286 288 415 4.88e-12 66.2
Msa0431890 AT3G21880.1 38.202 89 53 1 6 94 5 91 1.29e-11 64.7
Msa0431890 AT3G21880.3 38.202 89 53 1 6 94 9 95 1.41e-11 64.7
Msa0431890 AT3G21880.2 38.202 89 53 1 6 94 9 95 1.41e-11 64.7
Msa0431890 AT5G14370.1 63.043 46 17 0 232 277 294 339 1.94e-11 64.3
Msa0431890 AT4G25990.1 60.870 46 18 0 232 277 341 386 2.43e-11 63.9
Msa0431890 AT1G68520.1 68.182 44 14 0 232 275 357 400 2.60e-11 63.9
Msa0431890 AT5G60100.2 31.217 189 106 6 95 276 342 513 2.99e-11 63.9
Msa0431890 AT5G60100.5 31.217 189 106 6 95 276 315 486 3.42e-11 63.9
Msa0431890 AT5G60100.3 31.217 189 106 6 95 276 315 486 3.42e-11 63.9
Msa0431890 AT5G60100.1 31.217 189 106 6 95 276 315 486 3.42e-11 63.9

Find 81 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCAGTTGATTCCATTTC+TGG 0.107062 3_2:+84014224 Msa0431890:CDS
TCTTGCTTGCCAAACGATTT+TGG 0.181015 3_2:+84014399 Msa0431890:three_prime_UTR
ACAGAGTTTAGAAGCCATTT+TGG 0.192107 3_2:-84013288 None:intergenic
CAACATGCAAATCTCCAAAA+AGG 0.239203 3_2:-84014494 None:intergenic
GTAATAATGTTATGGTAAAA+AGG 0.244996 3_2:+84014432 Msa0431890:three_prime_UTR
GAAACTAGGCCTAGGATTAA+AGG 0.280004 3_2:+84014182 Msa0431890:CDS
AAACAAACTTCATTCCAAAA+TGG 0.295788 3_2:+84013274 Msa0431890:exon
CCGCAGAGGAAGGCGGAGTC+AGG 0.296878 3_2:-84013350 None:intergenic
TTCCGAAGCTATTCCGTTCA+TGG 0.298068 3_2:+84013721 Msa0431890:CDS
AGGCTAGGGTTATGAGGTAT+AGG 0.358381 3_2:+84014096 Msa0431890:CDS
GACGGTGTTGTTCCCGTTCA+TGG 0.369225 3_2:+84013776 Msa0431890:CDS
GCAAACCTCCCTTTAATCCT+AGG 0.392261 3_2:-84014191 None:intergenic
CCGTTCATGGATCTCGATTA+CGG 0.395344 3_2:+84013734 Msa0431890:CDS
TTTGTTAGCGGCGTGGACTT+TGG 0.416071 3_2:-84013384 None:intergenic
GGATCGAAGTTGCCATGAAC+GGG 0.430806 3_2:-84013788 None:intergenic
TGGCGGCGGATCGTGAGGCT+AGG 0.437396 3_2:+84014081 Msa0431890:CDS
GATTCGAAGAACGGAGTAAC+GGG 0.439450 3_2:-84013560 None:intergenic
AAATTCACCCCAAAATCGTT+TGG 0.440965 3_2:-84014408 None:intergenic
AGATATCGAACTGTGGTTAC+GGG 0.444139 3_2:+84014054 Msa0431890:CDS
GAGATATCGAACTGTGGTTA+CGG 0.445942 3_2:+84014053 Msa0431890:CDS
AACGATGCCGTATCCAGAAA+TGG 0.448783 3_2:-84014237 None:intergenic
GTTGATTCCATTTCTGGATA+CGG 0.451790 3_2:+84014230 Msa0431890:CDS
GATGAGTTGAGATCTGCTTT+CGG 0.461772 3_2:-84013680 None:intergenic
AGAAGCGTTGTTATGTGAAA+AGG 0.461814 3_2:-84013231 None:intergenic
AGGGTCTGAAAGACTGCAAC+CGG 0.468857 3_2:-84014474 None:intergenic
TCCGACACGGTGTTCCCGTC+AGG 0.479338 3_2:-84014032 None:intergenic
TTTAACAGAGTGAGAAGTGT+TGG 0.480554 3_2:-84013582 None:intergenic
GGCTAGGGTTATGAGGTATA+GGG 0.482522 3_2:+84014097 Msa0431890:CDS
TGGTTACGGGACGGTGGCGG+CGG 0.488159 3_2:+84014067 Msa0431890:CDS
AAACTAGGCCTAGGATTAAA+GGG 0.490422 3_2:+84014183 Msa0431890:CDS
AGTGTGGATGTCACGGTCAC+AGG 0.492186 3_2:-84013504 None:intergenic
ATCCATGAACGGAATAGCTT+CGG 0.492715 3_2:-84013723 None:intergenic
TTATGAGGTATAGGGAGAAG+AGG 0.496368 3_2:+84014105 Msa0431890:CDS
TTCTTGTAAGCTGAAACAAA+TGG 0.496560 3_2:-84013809 None:intergenic
GGCGGCGGATCGTGAGGCTA+GGG 0.502731 3_2:+84014082 Msa0431890:CDS
TTAGGTATCGTCTTCGTCGA+TGG 0.503016 3_2:+84013995 Msa0431890:intron
ATCTGTAATAACATTCGCTT+AGG 0.508607 3_2:+84014531 Msa0431890:three_prime_UTR
GTAAAGCATATGCGGAAACT+AGG 0.510714 3_2:+84014168 Msa0431890:CDS
CTGTGGTTACGGGACGGTGG+CGG 0.513006 3_2:+84014064 Msa0431890:CDS
CGCAGAGGAAGGCGGAGTCA+GGG 0.518288 3_2:-84013349 None:intergenic
TCGTCTTCGTCGATGGAGGT+TGG 0.523017 3_2:+84014002 Msa0431890:CDS
AGGTTGGAGTTGTTCCTGAC+GGG 0.524585 3_2:+84014018 Msa0431890:CDS
GGATTCGAAGAACGGAGTAA+CGG 0.536432 3_2:-84013561 None:intergenic
GCTTTACACGTGACATGTGC+AGG 0.538227 3_2:-84013458 None:intergenic
GGCGTGGACTTTGGAGTCAC+AGG 0.543388 3_2:-84013375 None:intergenic
GTCACAGGCGCCGCAGAGGA+AGG 0.545693 3_2:-84013360 None:intergenic
TCGGAATCAGAGAAAAGATA+AGG 0.547155 3_2:-84013704 None:intergenic
GACGGTGGCGGCGGATCGTG+AGG 0.549105 3_2:+84014076 Msa0431890:CDS
TGGATCGAAGTTGCCATGAA+CGG 0.558189 3_2:-84013789 None:intergenic
CGCTCGTGACGTGCGGCGAG+AGG 0.558417 3_2:-84013533 None:intergenic
ATGAAGCTGAAGTTGCTTCG+TGG 0.559796 3_2:+84013645 Msa0431890:CDS
GCAGTAGAGAGTAGCGGTGG+CGG 0.563031 3_2:-84013324 None:intergenic
GAAGTGTTGGATTCGAAGAA+CGG 0.565969 3_2:-84013569 None:intergenic
GATGCAGAGAGAAGCTGCAT+CGG 0.576026 3_2:-84013480 None:intergenic
CATATGCGGAAACTAGGCCT+AGG 0.577094 3_2:+84014174 Msa0431890:CDS
ACGAGAGGCGAGTTTGTTAG+CGG 0.583367 3_2:-84013396 None:intergenic
GGTATCGTCTTCGTCGATGG+AGG 0.585063 3_2:+84013998 Msa0431890:intron
ACAGGCGCCGCAGAGGAAGG+CGG 0.586506 3_2:-84013357 None:intergenic
GATTAGCAGTGTGGATGTCA+CGG 0.589251 3_2:-84013511 None:intergenic
CGCTTCTCGTAAAGCATATG+CGG 0.592961 3_2:+84014160 Msa0431890:CDS
GAGGTTGGAGTTGTTCCTGA+CGG 0.607148 3_2:+84014017 Msa0431890:CDS
GGGAACACGCTCGTGACGTG+CGG 0.610507 3_2:-84013540 None:intergenic
ATCGTGAGGCTAGGGTTATG+AGG 0.613658 3_2:+84014090 Msa0431890:CDS
TGGAGTCACAGGCGCCGCAG+AGG 0.616423 3_2:-84013364 None:intergenic
AGGCGAGTTTGTTAGCGGCG+TGG 0.630051 3_2:-84013391 None:intergenic
TGACTAATCTGCGACTGTGA+CGG 0.630286 3_2:-84013866 None:intergenic
AGTGACGCGAGGGTGACGAG+AGG 0.632213 3_2:-84013411 None:intergenic
GCGGCAGTAGAGAGTAGCGG+TGG 0.638763 3_2:-84013327 None:intergenic
AGGGCGGCAGTAGAGAGTAG+CGG 0.646899 3_2:-84013330 None:intergenic
CGGCGAGAGGATTAGCAGTG+TGG 0.659805 3_2:-84013520 None:intergenic
AGAGGAAGGCGGAGTCAGGG+CGG 0.663446 3_2:-84013346 None:intergenic
CCTGACTCCGCCTTCCTCTG+CGG 0.671751 3_2:+84013350 Msa0431890:CDS
ATCGAACTGTGGTTACGGGA+CGG 0.672790 3_2:+84014058 Msa0431890:CDS
TCCTGACGGGAACACCGTGT+CGG 0.682213 3_2:+84014031 Msa0431890:CDS
GAACTGTGGTTACGGGACGG+TGG 0.687113 3_2:+84014061 Msa0431890:CDS
CTTCGCAGAGAGTGACGCGA+GGG 0.694593 3_2:-84013421 None:intergenic
CTAGGCCTAGGATTAAAGGG+AGG 0.700310 3_2:+84014186 Msa0431890:CDS
ACTTCGCAGAGAGTGACGCG+AGG 0.710854 3_2:-84013422 None:intergenic
CCGTAATCGAGATCCATGAA+CGG 0.730276 3_2:-84013734 None:intergenic
ACAGTTCGATATCTCCGACA+CGG 0.744977 3_2:-84014045 None:intergenic
GTGTCGGAGATATCGAACTG+TGG 0.760977 3_2:+84014047 Msa0431890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATATTATAGTAATCAA+AGG + chr3_2:84014589-84014608 Msa0431890:three_prime_UTR 10.0%
!!! TTTTTTTGTTGTGATTAATT+AGG + chr3_2:84013977-84013996 Msa0431890:intron 15.0%
!!! TGAATTTTGTAATAATGTTA+TGG + chr3_2:84014424-84014443 Msa0431890:three_prime_UTR 15.0%
!!! TTTTTTTGTTGTGATTAATT+AGG + chr3_2:84013977-84013996 Msa0431890:intron 15.0%
!!! TGAATTTTGTAATAATGTTA+TGG + chr3_2:84014424-84014443 Msa0431890:three_prime_UTR 15.0%
!!! TAAGTTTGTTTTTATTGACA+TGG + chr3_2:84013918-84013937 Msa0431890:intron 20.0%
!! GTAATAATGTTATGGTAAAA+AGG + chr3_2:84014432-84014451 Msa0431890:three_prime_UTR 20.0%
!!! TAAGTTTGTTTTTATTGACA+TGG + chr3_2:84013918-84013937 Msa0431890:intron 20.0%
!! GTAATAATGTTATGGTAAAA+AGG + chr3_2:84014432-84014451 Msa0431890:three_prime_UTR 20.0%
!!! ATTTTGGAATGAAGTTTGTT+TGG - chr3_2:84013275-84013294 None:intergenic 25.0%
! AAACAAACTTCATTCCAAAA+TGG + chr3_2:84013274-84013293 Msa0431890:exon 25.0%
! GTAATTGAACATAAAAACGA+CGG + chr3_2:84013758-84013777 Msa0431890:CDS 25.0%
! CAAAAAAGAAATCAACAAGT+GGG - chr3_2:84014263-84014282 None:intergenic 25.0%
!!! ATTTTGGAATGAAGTTTGTT+TGG - chr3_2:84013275-84013294 None:intergenic 25.0%
! AAACAAACTTCATTCCAAAA+TGG + chr3_2:84013274-84013293 Msa0431890:exon 25.0%
! GTAATTGAACATAAAAACGA+CGG + chr3_2:84013758-84013777 Msa0431890:CDS 25.0%
! CAAAAAAGAAATCAACAAGT+GGG - chr3_2:84014263-84014282 None:intergenic 25.0%
TTCTTGTAAGCTGAAACAAA+TGG - chr3_2:84013812-84013831 None:intergenic 30.0%
GCAAAAAAGAAATCAACAAG+TGG - chr3_2:84014264-84014283 None:intergenic 30.0%
TATGGTAAAAAGGAAGATGA+TGG + chr3_2:84014442-84014461 Msa0431890:three_prime_UTR 30.0%
AACATGCAAATCTCCAAAAA+GGG - chr3_2:84014496-84014515 None:intergenic 30.0%
ATCTGTAATAACATTCGCTT+AGG + chr3_2:84014531-84014550 Msa0431890:three_prime_UTR 30.0%
TTCTTGTAAGCTGAAACAAA+TGG - chr3_2:84013812-84013831 None:intergenic 30.0%
GCAAAAAAGAAATCAACAAG+TGG - chr3_2:84014264-84014283 None:intergenic 30.0%
TATGGTAAAAAGGAAGATGA+TGG + chr3_2:84014442-84014461 Msa0431890:three_prime_UTR 30.0%
AACATGCAAATCTCCAAAAA+GGG - chr3_2:84014496-84014515 None:intergenic 30.0%
ATCTGTAATAACATTCGCTT+AGG + chr3_2:84014531-84014550 Msa0431890:three_prime_UTR 30.0%
!! AGAAGCGTTGTTATGTGAAA+AGG - chr3_2:84013234-84013253 None:intergenic 35.0%
! ACAGAGTTTAGAAGCCATTT+TGG - chr3_2:84013291-84013310 None:intergenic 35.0%
! TTTAACAGAGTGAGAAGTGT+TGG - chr3_2:84013585-84013604 None:intergenic 35.0%
TCGGAATCAGAGAAAAGATA+AGG - chr3_2:84013707-84013726 None:intergenic 35.0%
AAACTAGGCCTAGGATTAAA+GGG + chr3_2:84014183-84014202 Msa0431890:CDS 35.0%
! GTTGATTCCATTTCTGGATA+CGG + chr3_2:84014230-84014249 Msa0431890:CDS 35.0%
AAATTCACCCCAAAATCGTT+TGG - chr3_2:84014411-84014430 None:intergenic 35.0%
! AAGATGATGGCGAAAAATAC+CGG + chr3_2:84014455-84014474 Msa0431890:three_prime_UTR 35.0%
CAACATGCAAATCTCCAAAA+AGG - chr3_2:84014497-84014516 None:intergenic 35.0%
!! AGAAGCGTTGTTATGTGAAA+AGG - chr3_2:84013234-84013253 None:intergenic 35.0%
! ACAGAGTTTAGAAGCCATTT+TGG - chr3_2:84013291-84013310 None:intergenic 35.0%
! TTTAACAGAGTGAGAAGTGT+TGG - chr3_2:84013585-84013604 None:intergenic 35.0%
TCGGAATCAGAGAAAAGATA+AGG - chr3_2:84013707-84013726 None:intergenic 35.0%
AAACTAGGCCTAGGATTAAA+GGG + chr3_2:84014183-84014202 Msa0431890:CDS 35.0%
! GTTGATTCCATTTCTGGATA+CGG + chr3_2:84014230-84014249 Msa0431890:CDS 35.0%
AAATTCACCCCAAAATCGTT+TGG - chr3_2:84014411-84014430 None:intergenic 35.0%
! AAGATGATGGCGAAAAATAC+CGG + chr3_2:84014455-84014474 Msa0431890:three_prime_UTR 35.0%
CAACATGCAAATCTCCAAAA+AGG - chr3_2:84014497-84014516 None:intergenic 35.0%
!! GAAGTGTTGGATTCGAAGAA+CGG - chr3_2:84013572-84013591 None:intergenic 40.0%
! GATGAGTTGAGATCTGCTTT+CGG - chr3_2:84013683-84013702 None:intergenic 40.0%
ATCCATGAACGGAATAGCTT+CGG - chr3_2:84013726-84013745 None:intergenic 40.0%
GAGATATCGAACTGTGGTTA+CGG + chr3_2:84014053-84014072 Msa0431890:CDS 40.0%
AGATATCGAACTGTGGTTAC+GGG + chr3_2:84014054-84014073 Msa0431890:CDS 40.0%
TTATGAGGTATAGGGAGAAG+AGG + chr3_2:84014105-84014124 Msa0431890:CDS 40.0%
GTAAAGCATATGCGGAAACT+AGG + chr3_2:84014168-84014187 Msa0431890:CDS 40.0%
GAAACTAGGCCTAGGATTAA+AGG + chr3_2:84014182-84014201 Msa0431890:CDS 40.0%
! GATGCAGTTGATTCCATTTC+TGG + chr3_2:84014224-84014243 Msa0431890:CDS 40.0%
! TCTTGCTTGCCAAACGATTT+TGG + chr3_2:84014399-84014418 Msa0431890:three_prime_UTR 40.0%
! CTTGCTTGCCAAACGATTTT+GGG + chr3_2:84014400-84014419 Msa0431890:three_prime_UTR 40.0%
! TTGCTTGCCAAACGATTTTG+GGG + chr3_2:84014401-84014420 Msa0431890:three_prime_UTR 40.0%
!! GAAGTGTTGGATTCGAAGAA+CGG - chr3_2:84013572-84013591 None:intergenic 40.0%
! GATGAGTTGAGATCTGCTTT+CGG - chr3_2:84013683-84013702 None:intergenic 40.0%
ATCCATGAACGGAATAGCTT+CGG - chr3_2:84013726-84013745 None:intergenic 40.0%
GAGATATCGAACTGTGGTTA+CGG + chr3_2:84014053-84014072 Msa0431890:CDS 40.0%
AGATATCGAACTGTGGTTAC+GGG + chr3_2:84014054-84014073 Msa0431890:CDS 40.0%
TTATGAGGTATAGGGAGAAG+AGG + chr3_2:84014105-84014124 Msa0431890:CDS 40.0%
GTAAAGCATATGCGGAAACT+AGG + chr3_2:84014168-84014187 Msa0431890:CDS 40.0%
GAAACTAGGCCTAGGATTAA+AGG + chr3_2:84014182-84014201 Msa0431890:CDS 40.0%
! GATGCAGTTGATTCCATTTC+TGG + chr3_2:84014224-84014243 Msa0431890:CDS 40.0%
! TCTTGCTTGCCAAACGATTT+TGG + chr3_2:84014399-84014418 Msa0431890:three_prime_UTR 40.0%
! CTTGCTTGCCAAACGATTTT+GGG + chr3_2:84014400-84014419 Msa0431890:three_prime_UTR 40.0%
! TTGCTTGCCAAACGATTTTG+GGG + chr3_2:84014401-84014420 Msa0431890:three_prime_UTR 40.0%
GATTAGCAGTGTGGATGTCA+CGG - chr3_2:84013514-84013533 None:intergenic 45.0%
GATTCGAAGAACGGAGTAAC+GGG - chr3_2:84013563-84013582 None:intergenic 45.0%
GGATTCGAAGAACGGAGTAA+CGG - chr3_2:84013564-84013583 None:intergenic 45.0%
ATGAAGCTGAAGTTGCTTCG+TGG + chr3_2:84013645-84013664 Msa0431890:CDS 45.0%
TTCCGAAGCTATTCCGTTCA+TGG + chr3_2:84013721-84013740 Msa0431890:CDS 45.0%
CCGTAATCGAGATCCATGAA+CGG - chr3_2:84013737-84013756 None:intergenic 45.0%
CCGTTCATGGATCTCGATTA+CGG + chr3_2:84013734-84013753 Msa0431890:CDS 45.0%
TGGATCGAAGTTGCCATGAA+CGG - chr3_2:84013792-84013811 None:intergenic 45.0%
TGACTAATCTGCGACTGTGA+CGG - chr3_2:84013869-84013888 None:intergenic 45.0%
! TTAGGTATCGTCTTCGTCGA+TGG + chr3_2:84013995-84014014 Msa0431890:intron 45.0%
ACAGTTCGATATCTCCGACA+CGG - chr3_2:84014048-84014067 None:intergenic 45.0%
AGGCTAGGGTTATGAGGTAT+AGG + chr3_2:84014096-84014115 Msa0431890:CDS 45.0%
GGCTAGGGTTATGAGGTATA+GGG + chr3_2:84014097-84014116 Msa0431890:CDS 45.0%
CGCTTCTCGTAAAGCATATG+CGG + chr3_2:84014160-84014179 Msa0431890:CDS 45.0%
GCAAACCTCCCTTTAATCCT+AGG - chr3_2:84014194-84014213 None:intergenic 45.0%
AACGATGCCGTATCCAGAAA+TGG - chr3_2:84014240-84014259 None:intergenic 45.0%
! GCAGTCTTTCAGACCCTTTT+TGG + chr3_2:84014480-84014499 Msa0431890:three_prime_UTR 45.0%
GATTAGCAGTGTGGATGTCA+CGG - chr3_2:84013514-84013533 None:intergenic 45.0%
GATTCGAAGAACGGAGTAAC+GGG - chr3_2:84013563-84013582 None:intergenic 45.0%
GGATTCGAAGAACGGAGTAA+CGG - chr3_2:84013564-84013583 None:intergenic 45.0%
ATGAAGCTGAAGTTGCTTCG+TGG + chr3_2:84013645-84013664 Msa0431890:CDS 45.0%
TTCCGAAGCTATTCCGTTCA+TGG + chr3_2:84013721-84013740 Msa0431890:CDS 45.0%
CCGTAATCGAGATCCATGAA+CGG - chr3_2:84013737-84013756 None:intergenic 45.0%
CCGTTCATGGATCTCGATTA+CGG + chr3_2:84013734-84013753 Msa0431890:CDS 45.0%
TGGATCGAAGTTGCCATGAA+CGG - chr3_2:84013792-84013811 None:intergenic 45.0%
TGACTAATCTGCGACTGTGA+CGG - chr3_2:84013869-84013888 None:intergenic 45.0%
! TTAGGTATCGTCTTCGTCGA+TGG + chr3_2:84013995-84014014 Msa0431890:intron 45.0%
ACAGTTCGATATCTCCGACA+CGG - chr3_2:84014048-84014067 None:intergenic 45.0%
AGGCTAGGGTTATGAGGTAT+AGG + chr3_2:84014096-84014115 Msa0431890:CDS 45.0%
GGCTAGGGTTATGAGGTATA+GGG + chr3_2:84014097-84014116 Msa0431890:CDS 45.0%
CGCTTCTCGTAAAGCATATG+CGG + chr3_2:84014160-84014179 Msa0431890:CDS 45.0%
GCAAACCTCCCTTTAATCCT+AGG - chr3_2:84014194-84014213 None:intergenic 45.0%
AACGATGCCGTATCCAGAAA+TGG - chr3_2:84014240-84014259 None:intergenic 45.0%
! GCAGTCTTTCAGACCCTTTT+TGG + chr3_2:84014480-84014499 Msa0431890:three_prime_UTR 45.0%
TTTGTTAGCGGCGTGGACTT+TGG - chr3_2:84013387-84013406 None:intergenic 50.0%
!! ACGAGAGGCGAGTTTGTTAG+CGG - chr3_2:84013399-84013418 None:intergenic 50.0%
GCTTTACACGTGACATGTGC+AGG - chr3_2:84013461-84013480 None:intergenic 50.0%
GATGCAGAGAGAAGCTGCAT+CGG - chr3_2:84013483-84013502 None:intergenic 50.0%
GGATCGAAGTTGCCATGAAC+GGG - chr3_2:84013791-84013810 None:intergenic 50.0%
GAGGTTGGAGTTGTTCCTGA+CGG + chr3_2:84014017-84014036 Msa0431890:CDS 50.0%
AGGTTGGAGTTGTTCCTGAC+GGG + chr3_2:84014018-84014037 Msa0431890:CDS 50.0%
GTGTCGGAGATATCGAACTG+TGG + chr3_2:84014047-84014066 Msa0431890:CDS 50.0%
ATCGAACTGTGGTTACGGGA+CGG + chr3_2:84014058-84014077 Msa0431890:CDS 50.0%
ATCGTGAGGCTAGGGTTATG+AGG + chr3_2:84014090-84014109 Msa0431890:CDS 50.0%
CATATGCGGAAACTAGGCCT+AGG + chr3_2:84014174-84014193 Msa0431890:CDS 50.0%
CTAGGCCTAGGATTAAAGGG+AGG + chr3_2:84014186-84014205 Msa0431890:CDS 50.0%
AGGGTCTGAAAGACTGCAAC+CGG - chr3_2:84014477-84014496 None:intergenic 50.0%
TTTGTTAGCGGCGTGGACTT+TGG - chr3_2:84013387-84013406 None:intergenic 50.0%
!! ACGAGAGGCGAGTTTGTTAG+CGG - chr3_2:84013399-84013418 None:intergenic 50.0%
GCTTTACACGTGACATGTGC+AGG - chr3_2:84013461-84013480 None:intergenic 50.0%
GATGCAGAGAGAAGCTGCAT+CGG - chr3_2:84013483-84013502 None:intergenic 50.0%
GGATCGAAGTTGCCATGAAC+GGG - chr3_2:84013791-84013810 None:intergenic 50.0%
GAGGTTGGAGTTGTTCCTGA+CGG + chr3_2:84014017-84014036 Msa0431890:CDS 50.0%
AGGTTGGAGTTGTTCCTGAC+GGG + chr3_2:84014018-84014037 Msa0431890:CDS 50.0%
GTGTCGGAGATATCGAACTG+TGG + chr3_2:84014047-84014066 Msa0431890:CDS 50.0%
ATCGAACTGTGGTTACGGGA+CGG + chr3_2:84014058-84014077 Msa0431890:CDS 50.0%
ATCGTGAGGCTAGGGTTATG+AGG + chr3_2:84014090-84014109 Msa0431890:CDS 50.0%
CATATGCGGAAACTAGGCCT+AGG + chr3_2:84014174-84014193 Msa0431890:CDS 50.0%
CTAGGCCTAGGATTAAAGGG+AGG + chr3_2:84014186-84014205 Msa0431890:CDS 50.0%
AGGGTCTGAAAGACTGCAAC+CGG - chr3_2:84014477-84014496 None:intergenic 50.0%
AGTGTGGATGTCACGGTCAC+AGG - chr3_2:84013507-84013526 None:intergenic 55.0%
!! GACGGTGTTGTTCCCGTTCA+TGG + chr3_2:84013776-84013795 Msa0431890:CDS 55.0%
GGTATCGTCTTCGTCGATGG+AGG + chr3_2:84013998-84014017 Msa0431890:intron 55.0%
TCGTCTTCGTCGATGGAGGT+TGG + chr3_2:84014002-84014021 Msa0431890:CDS 55.0%
AGTGTGGATGTCACGGTCAC+AGG - chr3_2:84013507-84013526 None:intergenic 55.0%
!! GACGGTGTTGTTCCCGTTCA+TGG + chr3_2:84013776-84013795 Msa0431890:CDS 55.0%
GGTATCGTCTTCGTCGATGG+AGG + chr3_2:84013998-84014017 Msa0431890:intron 55.0%
TCGTCTTCGTCGATGGAGGT+TGG + chr3_2:84014002-84014021 Msa0431890:CDS 55.0%
GCAGTAGAGAGTAGCGGTGG+CGG - chr3_2:84013327-84013346 None:intergenic 60.0%
AGGGCGGCAGTAGAGAGTAG+CGG - chr3_2:84013333-84013352 None:intergenic 60.0%
!! GGCGTGGACTTTGGAGTCAC+AGG - chr3_2:84013378-84013397 None:intergenic 60.0%
!! AGGCGAGTTTGTTAGCGGCG+TGG - chr3_2:84013394-84013413 None:intergenic 60.0%
CTTCGCAGAGAGTGACGCGA+GGG - chr3_2:84013424-84013443 None:intergenic 60.0%
ACTTCGCAGAGAGTGACGCG+AGG - chr3_2:84013425-84013444 None:intergenic 60.0%
! CGGCGAGAGGATTAGCAGTG+TGG - chr3_2:84013523-84013542 None:intergenic 60.0%
TCCTGACGGGAACACCGTGT+CGG + chr3_2:84014031-84014050 Msa0431890:CDS 60.0%
GAACTGTGGTTACGGGACGG+TGG + chr3_2:84014061-84014080 Msa0431890:CDS 60.0%
GCAGTAGAGAGTAGCGGTGG+CGG - chr3_2:84013327-84013346 None:intergenic 60.0%
AGGGCGGCAGTAGAGAGTAG+CGG - chr3_2:84013333-84013352 None:intergenic 60.0%
!! GGCGTGGACTTTGGAGTCAC+AGG - chr3_2:84013378-84013397 None:intergenic 60.0%
!! AGGCGAGTTTGTTAGCGGCG+TGG - chr3_2:84013394-84013413 None:intergenic 60.0%
CTTCGCAGAGAGTGACGCGA+GGG - chr3_2:84013424-84013443 None:intergenic 60.0%
ACTTCGCAGAGAGTGACGCG+AGG - chr3_2:84013425-84013444 None:intergenic 60.0%
! CGGCGAGAGGATTAGCAGTG+TGG - chr3_2:84013523-84013542 None:intergenic 60.0%
TCCTGACGGGAACACCGTGT+CGG + chr3_2:84014031-84014050 Msa0431890:CDS 60.0%
GAACTGTGGTTACGGGACGG+TGG + chr3_2:84014061-84014080 Msa0431890:CDS 60.0%
GCGGCAGTAGAGAGTAGCGG+TGG - chr3_2:84013330-84013349 None:intergenic 65.0%
AGAGGAAGGCGGAGTCAGGG+CGG - chr3_2:84013349-84013368 None:intergenic 65.0%
CGCAGAGGAAGGCGGAGTCA+GGG - chr3_2:84013352-84013371 None:intergenic 65.0%
CCTGACTCCGCCTTCCTCTG+CGG + chr3_2:84013350-84013369 Msa0431890:CDS 65.0%
AGTGACGCGAGGGTGACGAG+AGG - chr3_2:84013414-84013433 None:intergenic 65.0%
GGGAACACGCTCGTGACGTG+CGG - chr3_2:84013543-84013562 None:intergenic 65.0%
TCCGACACGGTGTTCCCGTC+AGG - chr3_2:84014035-84014054 None:intergenic 65.0%
CTGTGGTTACGGGACGGTGG+CGG + chr3_2:84014064-84014083 Msa0431890:CDS 65.0%
GCGGCAGTAGAGAGTAGCGG+TGG - chr3_2:84013330-84013349 None:intergenic 65.0%
AGAGGAAGGCGGAGTCAGGG+CGG - chr3_2:84013349-84013368 None:intergenic 65.0%
CGCAGAGGAAGGCGGAGTCA+GGG - chr3_2:84013352-84013371 None:intergenic 65.0%
CCTGACTCCGCCTTCCTCTG+CGG + chr3_2:84013350-84013369 Msa0431890:CDS 65.0%
AGTGACGCGAGGGTGACGAG+AGG - chr3_2:84013414-84013433 None:intergenic 65.0%
GGGAACACGCTCGTGACGTG+CGG - chr3_2:84013543-84013562 None:intergenic 65.0%
TCCGACACGGTGTTCCCGTC+AGG - chr3_2:84014035-84014054 None:intergenic 65.0%
CTGTGGTTACGGGACGGTGG+CGG + chr3_2:84014064-84014083 Msa0431890:CDS 65.0%
CCGCAGAGGAAGGCGGAGTC+AGG - chr3_2:84013353-84013372 None:intergenic 70.0%
! ACAGGCGCCGCAGAGGAAGG+CGG - chr3_2:84013360-84013379 None:intergenic 70.0%
! GTCACAGGCGCCGCAGAGGA+AGG - chr3_2:84013363-84013382 None:intergenic 70.0%
! TGGAGTCACAGGCGCCGCAG+AGG - chr3_2:84013367-84013386 None:intergenic 70.0%
! TGGTTACGGGACGGTGGCGG+CGG + chr3_2:84014067-84014086 Msa0431890:CDS 70.0%
TGGCGGCGGATCGTGAGGCT+AGG + chr3_2:84014081-84014100 Msa0431890:CDS 70.0%
GGCGGCGGATCGTGAGGCTA+GGG + chr3_2:84014082-84014101 Msa0431890:CDS 70.0%
CCGCAGAGGAAGGCGGAGTC+AGG - chr3_2:84013353-84013372 None:intergenic 70.0%
! ACAGGCGCCGCAGAGGAAGG+CGG - chr3_2:84013360-84013379 None:intergenic 70.0%
! GTCACAGGCGCCGCAGAGGA+AGG - chr3_2:84013363-84013382 None:intergenic 70.0%
! TGGAGTCACAGGCGCCGCAG+AGG - chr3_2:84013367-84013386 None:intergenic 70.0%
! TGGTTACGGGACGGTGGCGG+CGG + chr3_2:84014067-84014086 Msa0431890:CDS 70.0%
TGGCGGCGGATCGTGAGGCT+AGG + chr3_2:84014081-84014100 Msa0431890:CDS 70.0%
GGCGGCGGATCGTGAGGCTA+GGG + chr3_2:84014082-84014101 Msa0431890:CDS 70.0%
CGCTCGTGACGTGCGGCGAG+AGG - chr3_2:84013536-84013555 None:intergenic 75.0%
! GACGGTGGCGGCGGATCGTG+AGG + chr3_2:84014076-84014095 Msa0431890:CDS 75.0%
CGCTCGTGACGTGCGGCGAG+AGG - chr3_2:84013536-84013555 None:intergenic 75.0%
! GACGGTGGCGGCGGATCGTG+AGG + chr3_2:84014076-84014095 Msa0431890:CDS 75.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 84013216 84014610 84013216 ID=Msa0431890;Name=Msa0431890
chr3_2 mRNA 84013216 84014610 84013216 ID=Msa0431890-mRNA-1;Parent=Msa0431890;Name=Msa0431890-mRNA-1;_AED=0.00;_eAED=0.00;_QI=77|1|1|1|1|1|2|339|290
chr3_2 exon 84013216 84013892 84013216 ID=Msa0431890-mRNA-1:exon:23634;Parent=Msa0431890-mRNA-1
chr3_2 exon 84013999 84014610 84013999 ID=Msa0431890-mRNA-1:exon:23635;Parent=Msa0431890-mRNA-1
chr3_2 five_prime_UTR 84013216 84013292 84013216 ID=Msa0431890-mRNA-1:five_prime_utr;Parent=Msa0431890-mRNA-1
chr3_2 CDS 84013293 84013892 84013293 ID=Msa0431890-mRNA-1:cds;Parent=Msa0431890-mRNA-1
chr3_2 CDS 84013999 84014271 84013999 ID=Msa0431890-mRNA-1:cds;Parent=Msa0431890-mRNA-1
chr3_2 three_prime_UTR 84014272 84014610 84014272 ID=Msa0431890-mRNA-1:three_prime_utr;Parent=Msa0431890-mRNA-1
Gene Sequence

>Msa0431890

ATGGCTTCTAAACTCTGTGACTCATGCAAATCCGCCACCGCTACTCTCTACTGCCGCCCTGACTCCGCCTTCCTCTGCGGCGCCTGTGACTCCAAAGTCCACGCCGCTAACAAACTCGCCTCTCGTCACCCTCGCGTCACTCTCTGCGAAGTCTGCGAACAAGCACCTGCACATGTCACGTGTAAAGCCGATGCAGCTTCTCTCTGCATCACCTGTGACCGTGACATCCACACTGCTAATCCTCTCGCCGCACGTCACGAGCGTGTTCCCGTTACTCCGTTCTTCGAATCCAACACTTCTCACTCTGTTAAATCGCTTCAAAACAATAACAACAACTACGATGCTGTTAAAGATGAAGCTGAAGTTGCTTCGTGGTTGATCTCTGATCCGAAAGCAGATCTCAACTCATCTCCTTATCTTTTCTCTGATTCCGAAGCTATTCCGTTCATGGATCTCGATTACGGTGTAATTGAACATAAAAACGACGGTGTTGTTCCCGTTCATGGCAACTTCGATCCATTTGTTTCAGCTTACAAGAACAACAACGTGCACTTGCATACAGAGTTAGAAACACCGTCACAGTCGCAGATTAGTCAGAGTGTATCGTCTTCGTCGATGGAGGTTGGAGTTGTTCCTGACGGGAACACCGTGTCGGAGATATCGAACTGTGGTTACGGGACGGTGGCGGCGGATCGTGAGGCTAGGGTTATGAGGTATAGGGAGAAGAGGAAGAATAGAAGATTCGAGAAGACGATTCGTTACGCTTCTCGTAAAGCATATGCGGAAACTAGGCCTAGGATTAAAGGGAGGTTTGCAAAACGCACTGATGCAGTTGATTCCATTTCTGGATACGGCATCGTTCCCACTTGTTGA
Protein sequence

>Msa0431890

MASKLCDSCKSATATLYCRPDSAFLCGACDSKVHAANKLASRHPRVTLCEVCEQAPAHVTCKADAASLCITCDRDIHTANPLAARHERVPVTPFFESNTSHSVKSLQNNNNNYDAVKDEAEVASWLISDPKADLNSSPYLFSDSEAIPFMDLDYGVIEHKNDGVVPVHGNFDPFVSAYKNNNVHLHTELETPSQSQISQSVSSSSMEVGVVPDGNTVSEISNCGYGTVAADREARVMRYREKRKNRRFEKTIRYASRKAYAETRPRIKGRFAKRTDAVDSISGYGIVPTC*