AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1368600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368600 MtrunA17_Chr3g0136701 97.586 290 7 0 1 290 1 290 0.0 590
Msa1368600 MtrunA17_Chr1g0150471 54.403 318 115 7 1 290 1 316 3.82e-106 311
Msa1368600 MtrunA17_Chr4g0072871 51.205 332 101 11 4 280 20 345 4.65e-89 270
Msa1368600 MtrunA17_Chr7g0220721 63.043 92 34 0 3 94 23 114 1.31e-37 137
Msa1368600 MtrunA17_Chr7g0220721 71.429 70 9 2 231 289 326 395 4.99e-23 97.4
Msa1368600 MtrunA17_Chr5g0405151 50.602 83 33 1 5 79 4 86 1.06e-20 87.4
Msa1368600 MtrunA17_Chr5g0429341 43.333 90 51 0 5 94 4 93 2.51e-19 87.4
Msa1368600 MtrunA17_Chr1g0155811 43.299 97 45 1 6 92 5 101 4.99e-19 84.7
Msa1368600 MtrunA17_Chr2g0314671 44.792 96 44 1 6 92 5 100 1.08e-18 84.0
Msa1368600 MtrunA17_Chr4g0033641 43.750 96 45 1 6 92 5 100 1.10e-18 83.2
Msa1368600 MtrunA17_Chr2g0329511 42.708 96 46 1 6 92 5 100 1.56e-17 80.1
Msa1368600 MtrunA17_Chr4g0035651 39.175 97 49 1 6 92 5 101 5.64e-17 78.2
Msa1368600 MtrunA17_Chr3g0145061 38.144 97 50 1 6 92 5 101 6.44e-17 78.2
Msa1368600 MtrunA17_Chr7g0268691 39.326 89 53 1 6 94 20 107 3.01e-16 78.2
Msa1368600 MtrunA17_Chr3g0120711 39.216 102 61 1 5 106 4 104 3.82e-16 78.2
Msa1368600 MtrunA17_Chr3g0120711 44.872 78 40 1 202 279 327 401 3.16e-11 63.5
Msa1368600 MtrunA17_Chr2g0322941 45.122 82 45 0 5 86 43 124 3.12e-15 73.6
Msa1368600 MtrunA17_Chr3g0142221 37.895 95 48 1 6 89 5 99 8.82e-15 72.0
Msa1368600 MtrunA17_Chr7g0251781 44.444 72 40 0 6 77 5 76 1.33e-13 70.5
Msa1368600 MtrunA17_Chr2g0322951 38.318 107 46 2 189 279 37 139 1.25e-12 64.3
Msa1368600 MtrunA17_Chr1g0209071 39.189 74 45 0 4 77 3 76 1.53e-12 67.0
Msa1368600 MtrunA17_Chr1g0209861 61.818 55 17 1 224 274 370 424 3.06e-12 66.6
Msa1368600 MtrunA17_Chr8g0390971 70.455 44 13 0 232 275 358 401 4.87e-12 65.9
Msa1368600 MtrunA17_Chr5g0431351 66.667 45 15 0 230 274 172 216 7.14e-12 63.5
Msa1368600 MtrunA17_Chr3g0127941 49.231 65 32 1 219 283 617 680 7.36e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368600 AT5G24930.1 52.000 375 80 13 1 289 1 361 6.65e-95 285
Msa1368600 AT2G24790.1 54.266 293 95 11 2 277 4 274 4.73e-94 280
Msa1368600 AT5G57660.1 42.775 346 131 10 2 289 18 354 2.34e-74 232
Msa1368600 AT3G02380.1 40.351 342 141 9 3 289 13 346 4.42e-73 228
Msa1368600 AT5G15850.1 41.210 347 143 8 3 289 9 354 5.84e-70 220
Msa1368600 AT5G15840.1 39.335 361 134 14 6 289 20 372 1.29e-65 210
Msa1368600 AT2G24790.2 56.180 178 55 6 2 171 4 166 8.17e-58 185
Msa1368600 AT5G15840.2 41.935 186 82 7 6 166 20 204 2.12e-37 134
Msa1368600 AT1G49130.1 28.435 313 176 10 4 280 26 326 1.65e-22 95.5
Msa1368600 AT1G49130.2 28.435 313 176 10 4 280 19 319 1.85e-22 95.5
Msa1368600 AT4G38960.1 35.570 149 74 3 5 138 4 145 3.41e-20 86.3
Msa1368600 AT4G38960.6 35.570 149 74 3 5 138 4 145 3.41e-20 86.3
Msa1368600 AT2G21320.1 49.398 83 34 1 5 79 4 86 6.48e-20 85.1
Msa1368600 AT1G78600.2 42.017 119 56 3 6 111 5 123 4.97e-19 85.9
Msa1368600 AT1G78600.1 42.017 119 56 3 6 111 5 123 5.28e-19 85.5
Msa1368600 AT2G47890.3 42.857 91 50 1 4 94 11 99 4.59e-18 82.8
Msa1368600 AT2G47890.1 42.857 91 50 1 4 94 11 99 5.13e-18 83.2
Msa1368600 AT2G47890.1 27.273 308 161 14 3 276 53 331 1.35e-13 70.5
Msa1368600 AT2G47890.2 42.857 91 50 1 4 94 11 99 6.31e-18 81.3
Msa1368600 AT1G06040.1 43.750 96 45 1 6 92 5 100 7.36e-18 81.3
Msa1368600 AT1G06040.2 43.750 96 45 1 6 92 5 100 1.01e-17 79.3
Msa1368600 AT1G75540.1 40.404 99 47 1 6 92 5 103 1.17e-17 82.0
Msa1368600 AT2G33500.1 42.697 89 51 0 6 94 12 100 3.25e-17 81.6
Msa1368600 AT2G33500.1 52.632 57 27 0 220 276 345 401 1.09e-12 68.2
Msa1368600 AT2G33500.2 42.697 89 51 0 6 94 12 100 3.26e-17 81.6
Msa1368600 AT2G33500.2 52.632 57 27 0 220 276 344 400 1.12e-12 68.2
Msa1368600 AT2G31380.1 34.177 158 87 4 6 153 5 155 1.44e-16 77.8
Msa1368600 AT1G28050.1 41.573 89 52 0 6 94 9 97 4.62e-16 78.2
Msa1368600 AT1G28050.1 56.000 50 22 0 228 277 381 430 9.23e-11 62.4
Msa1368600 AT5G48250.1 38.043 92 55 1 5 94 4 95 1.11e-14 73.9
Msa1368600 AT3G07650.3 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
Msa1368600 AT3G07650.3 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
Msa1368600 AT3G07650.2 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
Msa1368600 AT3G07650.2 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
Msa1368600 AT3G07650.4 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
Msa1368600 AT3G07650.4 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
Msa1368600 AT3G07650.1 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
Msa1368600 AT3G07650.1 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
Msa1368600 AT4G10240.1 33.714 175 81 7 6 165 5 159 4.33e-14 68.9
Msa1368600 AT4G39070.1 37.755 98 51 1 5 92 4 101 1.71e-13 68.9
Msa1368600 AT1G73870.1 55.556 63 27 1 213 275 327 388 2.52e-13 70.1
Msa1368600 AT4G15250.1 40.476 84 50 0 6 89 5 88 4.70e-13 68.9
Msa1368600 AT1G68190.2 38.824 85 50 1 4 88 41 123 6.48e-13 68.9
Msa1368600 AT1G68190.1 38.824 85 50 1 4 88 12 94 7.72e-13 68.6
Msa1368600 AT1G68190.3 38.824 85 50 1 4 88 12 94 7.72e-13 68.6
Msa1368600 AT1G25440.1 36.029 136 72 5 158 286 288 415 4.88e-12 66.2
Msa1368600 AT3G21880.1 38.202 89 53 1 6 94 5 91 1.29e-11 64.7
Msa1368600 AT3G21880.3 38.202 89 53 1 6 94 9 95 1.41e-11 64.7
Msa1368600 AT3G21880.2 38.202 89 53 1 6 94 9 95 1.41e-11 64.7
Msa1368600 AT5G14370.1 63.043 46 17 0 232 277 294 339 1.94e-11 64.3
Msa1368600 AT4G25990.1 60.870 46 18 0 232 277 341 386 2.43e-11 63.9
Msa1368600 AT1G68520.1 68.182 44 14 0 232 275 357 400 2.60e-11 63.9
Msa1368600 AT5G60100.2 31.217 189 106 6 95 276 342 513 2.99e-11 63.9
Msa1368600 AT5G60100.5 31.217 189 106 6 95 276 315 486 3.42e-11 63.9
Msa1368600 AT5G60100.3 31.217 189 106 6 95 276 315 486 3.42e-11 63.9
Msa1368600 AT5G60100.1 31.217 189 106 6 95 276 315 486 3.42e-11 63.9

Find 80 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCAGTTGATTCCATTTC+TGG 0.107062 tig0018650:+66944 Msa1368600:CDS
TCTTGCTTGCCAAACGATTT+TGG 0.181015 tig0018650:+67119 Msa1368600:three_prime_UTR
ACAGAGTTTAGAAGCCATTT+TGG 0.192107 tig0018650:-66008 None:intergenic
CAACATGCAAATCTCCAAAA+AGG 0.239203 tig0018650:-67214 None:intergenic
GTAATAATGTTATGGTAAAA+AGG 0.244996 tig0018650:+67152 Msa1368600:three_prime_UTR
GAAACTAGGCCTAGGATTAA+AGG 0.280004 tig0018650:+66902 Msa1368600:CDS
AAACAAACTTCATTCCAAAA+TGG 0.295788 tig0018650:+65994 Msa1368600:exon
CCGCAGAGGAAGGCGGAGTC+AGG 0.296878 tig0018650:-66070 None:intergenic
TTCCGAAGCTATTCCGTTCA+TGG 0.298068 tig0018650:+66441 Msa1368600:CDS
AAGCTAGGGTTATGAGGTAT+AGG 0.350898 tig0018650:+66816 Msa1368600:CDS
GACGGTGTTGTTCCCGTTCA+TGG 0.369225 tig0018650:+66496 Msa1368600:CDS
GCAAACCTCCCTTTAATCCT+AGG 0.392261 tig0018650:-66911 None:intergenic
CCGTTCATGGATCTCGATTA+CGG 0.395344 tig0018650:+66454 Msa1368600:CDS
TTTGTTAGCGGCGTGGACTT+TGG 0.416071 tig0018650:-66104 None:intergenic
GGATCGAAGTTGCCATGAAC+GGG 0.430806 tig0018650:-66508 None:intergenic
TGGCGGCGGATCGTGAAGCT+AGG 0.432649 tig0018650:+66801 Msa1368600:CDS
GATTCGAAGAACGGAGTAAC+GGG 0.439450 tig0018650:-66280 None:intergenic
AGCTAGGGTTATGAGGTATA+GGG 0.439658 tig0018650:+66817 Msa1368600:CDS
AAATTCACCCCAAAATCGTT+TGG 0.440965 tig0018650:-67128 None:intergenic
AGATATCGAACTGTGGTTAC+GGG 0.444139 tig0018650:+66774 Msa1368600:CDS
GAGATATCGAACTGTGGTTA+CGG 0.445942 tig0018650:+66773 Msa1368600:CDS
AACGATGCCGTATCCAGAAA+TGG 0.448783 tig0018650:-66957 None:intergenic
GTTGATTCCATTTCTGGATA+CGG 0.451790 tig0018650:+66950 Msa1368600:CDS
GATGAGTTGAGATCTGCTTT+CGG 0.461772 tig0018650:-66400 None:intergenic
AGAAGCGTTGTTATGTGAAA+AGG 0.461814 tig0018650:-65948 None:intergenic
AGGGTCTGAAAGACTGCAAC+CGG 0.468857 tig0018650:-67194 None:intergenic
TCCGACACGGTGTTCCCGTC+AGG 0.479338 tig0018650:-66752 None:intergenic
TTTAACAGAGTGAGAAGTGT+TGG 0.480554 tig0018650:-66302 None:intergenic
TGGTTACGGGACGGTGGCGG+CGG 0.488159 tig0018650:+66787 Msa1368600:CDS
AAACTAGGCCTAGGATTAAA+GGG 0.490422 tig0018650:+66903 Msa1368600:CDS
AGTGTGGATGTCACGGTCAC+AGG 0.492186 tig0018650:-66224 None:intergenic
ATCCATGAACGGAATAGCTT+CGG 0.492715 tig0018650:-66443 None:intergenic
TTATGAGGTATAGGGAGAAG+AGG 0.496368 tig0018650:+66825 Msa1368600:CDS
TTCTTGTAAGCTGAAACAAA+TGG 0.496560 tig0018650:-66529 None:intergenic
TTAGGTATCGTCTTCGTCGA+TGG 0.503016 tig0018650:+66715 Msa1368600:intron
ATCTGTAATAACATTCGCTT+AGG 0.508607 tig0018650:+67251 Msa1368600:three_prime_UTR
GTAAAGCATATGCGGAAACT+AGG 0.510714 tig0018650:+66888 Msa1368600:CDS
CTGTGGTTACGGGACGGTGG+CGG 0.513006 tig0018650:+66784 Msa1368600:CDS
CGCAGAGGAAGGCGGAGTCA+GGG 0.518288 tig0018650:-66069 None:intergenic
TCGTCTTCGTCGATGGAGGT+TGG 0.523017 tig0018650:+66722 Msa1368600:CDS
AGGTTGGAGTTGTTCCTGAC+GGG 0.524585 tig0018650:+66738 Msa1368600:CDS
GGATTCGAAGAACGGAGTAA+CGG 0.536432 tig0018650:-66281 None:intergenic
GCTTTACACGTGACATGTGC+AGG 0.538227 tig0018650:-66178 None:intergenic
GGCGTGGACTTTGGAGTCAC+AGG 0.543388 tig0018650:-66095 None:intergenic
GTCACAGGCGCCGCAGAGGA+AGG 0.545693 tig0018650:-66080 None:intergenic
TCGGAATCAGAGAAAAGATA+AGG 0.547155 tig0018650:-66424 None:intergenic
TGGATCGAAGTTGCCATGAA+CGG 0.558189 tig0018650:-66509 None:intergenic
CGCTCGTGACGTGCGGCGAG+AGG 0.558417 tig0018650:-66253 None:intergenic
ATGAAGCTGAAGTTGCTTCG+TGG 0.559796 tig0018650:+66365 Msa1368600:CDS
GCAGTAGAGAGTAGCGGTGG+CGG 0.563031 tig0018650:-66044 None:intergenic
GAAGTGTTGGATTCGAAGAA+CGG 0.565969 tig0018650:-66289 None:intergenic
GATGCAGAGAGAAGCTGCAT+CGG 0.576026 tig0018650:-66200 None:intergenic
CATATGCGGAAACTAGGCCT+AGG 0.577094 tig0018650:+66894 Msa1368600:CDS
GGCGGCGGATCGTGAAGCTA+GGG 0.577274 tig0018650:+66802 Msa1368600:CDS
ACGAGAGGCGAGTTTGTTAG+CGG 0.583367 tig0018650:-66116 None:intergenic
GGTATCGTCTTCGTCGATGG+AGG 0.585063 tig0018650:+66718 Msa1368600:intron
ACAGGCGCCGCAGAGGAAGG+CGG 0.586506 tig0018650:-66077 None:intergenic
GATTAGCAGTGTGGATGTCA+CGG 0.589251 tig0018650:-66231 None:intergenic
CGCTTCTCGTAAAGCATATG+CGG 0.592961 tig0018650:+66880 Msa1368600:CDS
GAGGTTGGAGTTGTTCCTGA+CGG 0.607148 tig0018650:+66737 Msa1368600:CDS
GGGAACACGCTCGTGACGTG+CGG 0.610507 tig0018650:-66260 None:intergenic
ATCGTGAAGCTAGGGTTATG+AGG 0.615678 tig0018650:+66810 Msa1368600:CDS
TGGAGTCACAGGCGCCGCAG+AGG 0.616423 tig0018650:-66084 None:intergenic
AGGCGAGTTTGTTAGCGGCG+TGG 0.630051 tig0018650:-66111 None:intergenic
TGACTAATCTGCGACTGTGA+CGG 0.630286 tig0018650:-66586 None:intergenic
AGTGACGCGAGGGTGACGAG+AGG 0.632213 tig0018650:-66131 None:intergenic
GCGGCAGTAGAGAGTAGCGG+TGG 0.638763 tig0018650:-66047 None:intergenic
AGGGCGGCAGTAGAGAGTAG+CGG 0.646899 tig0018650:-66050 None:intergenic
CGGCGAGAGGATTAGCAGTG+TGG 0.659805 tig0018650:-66240 None:intergenic
AGAGGAAGGCGGAGTCAGGG+CGG 0.663446 tig0018650:-66066 None:intergenic
CCTGACTCCGCCTTCCTCTG+CGG 0.671751 tig0018650:+66070 Msa1368600:CDS
ATCGAACTGTGGTTACGGGA+CGG 0.672790 tig0018650:+66778 Msa1368600:CDS
TCCTGACGGGAACACCGTGT+CGG 0.682213 tig0018650:+66751 Msa1368600:CDS
GAACTGTGGTTACGGGACGG+TGG 0.687113 tig0018650:+66781 Msa1368600:CDS
CTTCGCAGAGAGTGACGCGA+GGG 0.694593 tig0018650:-66141 None:intergenic
CTAGGCCTAGGATTAAAGGG+AGG 0.700310 tig0018650:+66906 Msa1368600:CDS
ACTTCGCAGAGAGTGACGCG+AGG 0.710854 tig0018650:-66142 None:intergenic
CCGTAATCGAGATCCATGAA+CGG 0.730276 tig0018650:-66454 None:intergenic
ACAGTTCGATATCTCCGACA+CGG 0.744977 tig0018650:-66765 None:intergenic
GTGTCGGAGATATCGAACTG+TGG 0.760977 tig0018650:+66767 Msa1368600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATATTATAGTAATCAA+AGG + tig0018650:67309-67328 Msa1368600:three_prime_UTR 10.0%
!!! TTTTTTTGTTGTGATTAATT+AGG + tig0018650:66697-66716 Msa1368600:intron 15.0%
!!! TGAATTTTGTAATAATGTTA+TGG + tig0018650:67144-67163 Msa1368600:three_prime_UTR 15.0%
!!! TTTTTTTGTTGTGATTAATT+AGG + tig0018650:66697-66716 Msa1368600:intron 15.0%
!!! TGAATTTTGTAATAATGTTA+TGG + tig0018650:67144-67163 Msa1368600:three_prime_UTR 15.0%
!!! TAAGTTTGTTTTTATTGACA+TGG + tig0018650:66638-66657 Msa1368600:intron 20.0%
!! GTAATAATGTTATGGTAAAA+AGG + tig0018650:67152-67171 Msa1368600:three_prime_UTR 20.0%
!!! TAAGTTTGTTTTTATTGACA+TGG + tig0018650:66638-66657 Msa1368600:intron 20.0%
!! GTAATAATGTTATGGTAAAA+AGG + tig0018650:67152-67171 Msa1368600:three_prime_UTR 20.0%
!!! ATTTTGGAATGAAGTTTGTT+TGG - tig0018650:65995-66014 None:intergenic 25.0%
! AAACAAACTTCATTCCAAAA+TGG + tig0018650:65994-66013 Msa1368600:exon 25.0%
! GTAATTGAACATAAAAACGA+CGG + tig0018650:66478-66497 Msa1368600:CDS 25.0%
! CAAAAAAGAAATCAACAAGT+GGG - tig0018650:66983-67002 None:intergenic 25.0%
!!! ATTTTGGAATGAAGTTTGTT+TGG - tig0018650:65995-66014 None:intergenic 25.0%
! AAACAAACTTCATTCCAAAA+TGG + tig0018650:65994-66013 Msa1368600:exon 25.0%
! GTAATTGAACATAAAAACGA+CGG + tig0018650:66478-66497 Msa1368600:CDS 25.0%
! CAAAAAAGAAATCAACAAGT+GGG - tig0018650:66983-67002 None:intergenic 25.0%
TTCTTGTAAGCTGAAACAAA+TGG - tig0018650:66532-66551 None:intergenic 30.0%
GCAAAAAAGAAATCAACAAG+TGG - tig0018650:66984-67003 None:intergenic 30.0%
TATGGTAAAAAGGAAGATGA+TGG + tig0018650:67162-67181 Msa1368600:three_prime_UTR 30.0%
AACATGCAAATCTCCAAAAA+GGG - tig0018650:67216-67235 None:intergenic 30.0%
ATCTGTAATAACATTCGCTT+AGG + tig0018650:67251-67270 Msa1368600:three_prime_UTR 30.0%
TTCTTGTAAGCTGAAACAAA+TGG - tig0018650:66532-66551 None:intergenic 30.0%
GCAAAAAAGAAATCAACAAG+TGG - tig0018650:66984-67003 None:intergenic 30.0%
TATGGTAAAAAGGAAGATGA+TGG + tig0018650:67162-67181 Msa1368600:three_prime_UTR 30.0%
AACATGCAAATCTCCAAAAA+GGG - tig0018650:67216-67235 None:intergenic 30.0%
ATCTGTAATAACATTCGCTT+AGG + tig0018650:67251-67270 Msa1368600:three_prime_UTR 30.0%
!! AGAAGCGTTGTTATGTGAAA+AGG - tig0018650:65951-65970 None:intergenic 35.0%
! ACAGAGTTTAGAAGCCATTT+TGG - tig0018650:66011-66030 None:intergenic 35.0%
! TTTAACAGAGTGAGAAGTGT+TGG - tig0018650:66305-66324 None:intergenic 35.0%
TCGGAATCAGAGAAAAGATA+AGG - tig0018650:66427-66446 None:intergenic 35.0%
AAACTAGGCCTAGGATTAAA+GGG + tig0018650:66903-66922 Msa1368600:CDS 35.0%
! GTTGATTCCATTTCTGGATA+CGG + tig0018650:66950-66969 Msa1368600:CDS 35.0%
AAATTCACCCCAAAATCGTT+TGG - tig0018650:67131-67150 None:intergenic 35.0%
! AAGATGATGGCGAAAAATAC+CGG + tig0018650:67175-67194 Msa1368600:three_prime_UTR 35.0%
CAACATGCAAATCTCCAAAA+AGG - tig0018650:67217-67236 None:intergenic 35.0%
!! AGAAGCGTTGTTATGTGAAA+AGG - tig0018650:65951-65970 None:intergenic 35.0%
! ACAGAGTTTAGAAGCCATTT+TGG - tig0018650:66011-66030 None:intergenic 35.0%
! TTTAACAGAGTGAGAAGTGT+TGG - tig0018650:66305-66324 None:intergenic 35.0%
TCGGAATCAGAGAAAAGATA+AGG - tig0018650:66427-66446 None:intergenic 35.0%
AAACTAGGCCTAGGATTAAA+GGG + tig0018650:66903-66922 Msa1368600:CDS 35.0%
! GTTGATTCCATTTCTGGATA+CGG + tig0018650:66950-66969 Msa1368600:CDS 35.0%
AAATTCACCCCAAAATCGTT+TGG - tig0018650:67131-67150 None:intergenic 35.0%
! AAGATGATGGCGAAAAATAC+CGG + tig0018650:67175-67194 Msa1368600:three_prime_UTR 35.0%
CAACATGCAAATCTCCAAAA+AGG - tig0018650:67217-67236 None:intergenic 35.0%
!! GAAGTGTTGGATTCGAAGAA+CGG - tig0018650:66292-66311 None:intergenic 40.0%
! GATGAGTTGAGATCTGCTTT+CGG - tig0018650:66403-66422 None:intergenic 40.0%
ATCCATGAACGGAATAGCTT+CGG - tig0018650:66446-66465 None:intergenic 40.0%
GAGATATCGAACTGTGGTTA+CGG + tig0018650:66773-66792 Msa1368600:CDS 40.0%
AGATATCGAACTGTGGTTAC+GGG + tig0018650:66774-66793 Msa1368600:CDS 40.0%
AAGCTAGGGTTATGAGGTAT+AGG + tig0018650:66816-66835 Msa1368600:CDS 40.0%
AGCTAGGGTTATGAGGTATA+GGG + tig0018650:66817-66836 Msa1368600:CDS 40.0%
TTATGAGGTATAGGGAGAAG+AGG + tig0018650:66825-66844 Msa1368600:CDS 40.0%
GTAAAGCATATGCGGAAACT+AGG + tig0018650:66888-66907 Msa1368600:CDS 40.0%
GAAACTAGGCCTAGGATTAA+AGG + tig0018650:66902-66921 Msa1368600:CDS 40.0%
! GATGCAGTTGATTCCATTTC+TGG + tig0018650:66944-66963 Msa1368600:CDS 40.0%
! TCTTGCTTGCCAAACGATTT+TGG + tig0018650:67119-67138 Msa1368600:three_prime_UTR 40.0%
! CTTGCTTGCCAAACGATTTT+GGG + tig0018650:67120-67139 Msa1368600:three_prime_UTR 40.0%
! TTGCTTGCCAAACGATTTTG+GGG + tig0018650:67121-67140 Msa1368600:three_prime_UTR 40.0%
!! GAAGTGTTGGATTCGAAGAA+CGG - tig0018650:66292-66311 None:intergenic 40.0%
! GATGAGTTGAGATCTGCTTT+CGG - tig0018650:66403-66422 None:intergenic 40.0%
ATCCATGAACGGAATAGCTT+CGG - tig0018650:66446-66465 None:intergenic 40.0%
GAGATATCGAACTGTGGTTA+CGG + tig0018650:66773-66792 Msa1368600:CDS 40.0%
AGATATCGAACTGTGGTTAC+GGG + tig0018650:66774-66793 Msa1368600:CDS 40.0%
AAGCTAGGGTTATGAGGTAT+AGG + tig0018650:66816-66835 Msa1368600:CDS 40.0%
AGCTAGGGTTATGAGGTATA+GGG + tig0018650:66817-66836 Msa1368600:CDS 40.0%
TTATGAGGTATAGGGAGAAG+AGG + tig0018650:66825-66844 Msa1368600:CDS 40.0%
GTAAAGCATATGCGGAAACT+AGG + tig0018650:66888-66907 Msa1368600:CDS 40.0%
GAAACTAGGCCTAGGATTAA+AGG + tig0018650:66902-66921 Msa1368600:CDS 40.0%
! GATGCAGTTGATTCCATTTC+TGG + tig0018650:66944-66963 Msa1368600:CDS 40.0%
! TCTTGCTTGCCAAACGATTT+TGG + tig0018650:67119-67138 Msa1368600:three_prime_UTR 40.0%
! CTTGCTTGCCAAACGATTTT+GGG + tig0018650:67120-67139 Msa1368600:three_prime_UTR 40.0%
! TTGCTTGCCAAACGATTTTG+GGG + tig0018650:67121-67140 Msa1368600:three_prime_UTR 40.0%
GATTAGCAGTGTGGATGTCA+CGG - tig0018650:66234-66253 None:intergenic 45.0%
GATTCGAAGAACGGAGTAAC+GGG - tig0018650:66283-66302 None:intergenic 45.0%
GGATTCGAAGAACGGAGTAA+CGG - tig0018650:66284-66303 None:intergenic 45.0%
ATGAAGCTGAAGTTGCTTCG+TGG + tig0018650:66365-66384 Msa1368600:CDS 45.0%
TTCCGAAGCTATTCCGTTCA+TGG + tig0018650:66441-66460 Msa1368600:CDS 45.0%
CCGTAATCGAGATCCATGAA+CGG - tig0018650:66457-66476 None:intergenic 45.0%
CCGTTCATGGATCTCGATTA+CGG + tig0018650:66454-66473 Msa1368600:CDS 45.0%
TGGATCGAAGTTGCCATGAA+CGG - tig0018650:66512-66531 None:intergenic 45.0%
TGACTAATCTGCGACTGTGA+CGG - tig0018650:66589-66608 None:intergenic 45.0%
! TTAGGTATCGTCTTCGTCGA+TGG + tig0018650:66715-66734 Msa1368600:intron 45.0%
ACAGTTCGATATCTCCGACA+CGG - tig0018650:66768-66787 None:intergenic 45.0%
ATCGTGAAGCTAGGGTTATG+AGG + tig0018650:66810-66829 Msa1368600:CDS 45.0%
CGCTTCTCGTAAAGCATATG+CGG + tig0018650:66880-66899 Msa1368600:CDS 45.0%
GCAAACCTCCCTTTAATCCT+AGG - tig0018650:66914-66933 None:intergenic 45.0%
AACGATGCCGTATCCAGAAA+TGG - tig0018650:66960-66979 None:intergenic 45.0%
! GCAGTCTTTCAGACCCTTTT+TGG + tig0018650:67200-67219 Msa1368600:three_prime_UTR 45.0%
GATTAGCAGTGTGGATGTCA+CGG - tig0018650:66234-66253 None:intergenic 45.0%
GATTCGAAGAACGGAGTAAC+GGG - tig0018650:66283-66302 None:intergenic 45.0%
GGATTCGAAGAACGGAGTAA+CGG - tig0018650:66284-66303 None:intergenic 45.0%
ATGAAGCTGAAGTTGCTTCG+TGG + tig0018650:66365-66384 Msa1368600:CDS 45.0%
TTCCGAAGCTATTCCGTTCA+TGG + tig0018650:66441-66460 Msa1368600:CDS 45.0%
CCGTAATCGAGATCCATGAA+CGG - tig0018650:66457-66476 None:intergenic 45.0%
CCGTTCATGGATCTCGATTA+CGG + tig0018650:66454-66473 Msa1368600:CDS 45.0%
TGGATCGAAGTTGCCATGAA+CGG - tig0018650:66512-66531 None:intergenic 45.0%
TGACTAATCTGCGACTGTGA+CGG - tig0018650:66589-66608 None:intergenic 45.0%
! TTAGGTATCGTCTTCGTCGA+TGG + tig0018650:66715-66734 Msa1368600:intron 45.0%
ACAGTTCGATATCTCCGACA+CGG - tig0018650:66768-66787 None:intergenic 45.0%
ATCGTGAAGCTAGGGTTATG+AGG + tig0018650:66810-66829 Msa1368600:CDS 45.0%
CGCTTCTCGTAAAGCATATG+CGG + tig0018650:66880-66899 Msa1368600:CDS 45.0%
GCAAACCTCCCTTTAATCCT+AGG - tig0018650:66914-66933 None:intergenic 45.0%
AACGATGCCGTATCCAGAAA+TGG - tig0018650:66960-66979 None:intergenic 45.0%
! GCAGTCTTTCAGACCCTTTT+TGG + tig0018650:67200-67219 Msa1368600:three_prime_UTR 45.0%
TTTGTTAGCGGCGTGGACTT+TGG - tig0018650:66107-66126 None:intergenic 50.0%
!! ACGAGAGGCGAGTTTGTTAG+CGG - tig0018650:66119-66138 None:intergenic 50.0%
GCTTTACACGTGACATGTGC+AGG - tig0018650:66181-66200 None:intergenic 50.0%
GATGCAGAGAGAAGCTGCAT+CGG - tig0018650:66203-66222 None:intergenic 50.0%
GGATCGAAGTTGCCATGAAC+GGG - tig0018650:66511-66530 None:intergenic 50.0%
GAGGTTGGAGTTGTTCCTGA+CGG + tig0018650:66737-66756 Msa1368600:CDS 50.0%
AGGTTGGAGTTGTTCCTGAC+GGG + tig0018650:66738-66757 Msa1368600:CDS 50.0%
GTGTCGGAGATATCGAACTG+TGG + tig0018650:66767-66786 Msa1368600:CDS 50.0%
ATCGAACTGTGGTTACGGGA+CGG + tig0018650:66778-66797 Msa1368600:CDS 50.0%
CATATGCGGAAACTAGGCCT+AGG + tig0018650:66894-66913 Msa1368600:CDS 50.0%
CTAGGCCTAGGATTAAAGGG+AGG + tig0018650:66906-66925 Msa1368600:CDS 50.0%
AGGGTCTGAAAGACTGCAAC+CGG - tig0018650:67197-67216 None:intergenic 50.0%
TTTGTTAGCGGCGTGGACTT+TGG - tig0018650:66107-66126 None:intergenic 50.0%
!! ACGAGAGGCGAGTTTGTTAG+CGG - tig0018650:66119-66138 None:intergenic 50.0%
GCTTTACACGTGACATGTGC+AGG - tig0018650:66181-66200 None:intergenic 50.0%
GATGCAGAGAGAAGCTGCAT+CGG - tig0018650:66203-66222 None:intergenic 50.0%
GGATCGAAGTTGCCATGAAC+GGG - tig0018650:66511-66530 None:intergenic 50.0%
GAGGTTGGAGTTGTTCCTGA+CGG + tig0018650:66737-66756 Msa1368600:CDS 50.0%
AGGTTGGAGTTGTTCCTGAC+GGG + tig0018650:66738-66757 Msa1368600:CDS 50.0%
GTGTCGGAGATATCGAACTG+TGG + tig0018650:66767-66786 Msa1368600:CDS 50.0%
ATCGAACTGTGGTTACGGGA+CGG + tig0018650:66778-66797 Msa1368600:CDS 50.0%
CATATGCGGAAACTAGGCCT+AGG + tig0018650:66894-66913 Msa1368600:CDS 50.0%
CTAGGCCTAGGATTAAAGGG+AGG + tig0018650:66906-66925 Msa1368600:CDS 50.0%
AGGGTCTGAAAGACTGCAAC+CGG - tig0018650:67197-67216 None:intergenic 50.0%
AGTGTGGATGTCACGGTCAC+AGG - tig0018650:66227-66246 None:intergenic 55.0%
!! GACGGTGTTGTTCCCGTTCA+TGG + tig0018650:66496-66515 Msa1368600:CDS 55.0%
GGTATCGTCTTCGTCGATGG+AGG + tig0018650:66718-66737 Msa1368600:intron 55.0%
TCGTCTTCGTCGATGGAGGT+TGG + tig0018650:66722-66741 Msa1368600:CDS 55.0%
AGTGTGGATGTCACGGTCAC+AGG - tig0018650:66227-66246 None:intergenic 55.0%
!! GACGGTGTTGTTCCCGTTCA+TGG + tig0018650:66496-66515 Msa1368600:CDS 55.0%
GGTATCGTCTTCGTCGATGG+AGG + tig0018650:66718-66737 Msa1368600:intron 55.0%
TCGTCTTCGTCGATGGAGGT+TGG + tig0018650:66722-66741 Msa1368600:CDS 55.0%
GCAGTAGAGAGTAGCGGTGG+CGG - tig0018650:66047-66066 None:intergenic 60.0%
AGGGCGGCAGTAGAGAGTAG+CGG - tig0018650:66053-66072 None:intergenic 60.0%
!! GGCGTGGACTTTGGAGTCAC+AGG - tig0018650:66098-66117 None:intergenic 60.0%
!! AGGCGAGTTTGTTAGCGGCG+TGG - tig0018650:66114-66133 None:intergenic 60.0%
CTTCGCAGAGAGTGACGCGA+GGG - tig0018650:66144-66163 None:intergenic 60.0%
ACTTCGCAGAGAGTGACGCG+AGG - tig0018650:66145-66164 None:intergenic 60.0%
! CGGCGAGAGGATTAGCAGTG+TGG - tig0018650:66243-66262 None:intergenic 60.0%
TCCTGACGGGAACACCGTGT+CGG + tig0018650:66751-66770 Msa1368600:CDS 60.0%
GAACTGTGGTTACGGGACGG+TGG + tig0018650:66781-66800 Msa1368600:CDS 60.0%
GCAGTAGAGAGTAGCGGTGG+CGG - tig0018650:66047-66066 None:intergenic 60.0%
AGGGCGGCAGTAGAGAGTAG+CGG - tig0018650:66053-66072 None:intergenic 60.0%
!! GGCGTGGACTTTGGAGTCAC+AGG - tig0018650:66098-66117 None:intergenic 60.0%
!! AGGCGAGTTTGTTAGCGGCG+TGG - tig0018650:66114-66133 None:intergenic 60.0%
CTTCGCAGAGAGTGACGCGA+GGG - tig0018650:66144-66163 None:intergenic 60.0%
ACTTCGCAGAGAGTGACGCG+AGG - tig0018650:66145-66164 None:intergenic 60.0%
! CGGCGAGAGGATTAGCAGTG+TGG - tig0018650:66243-66262 None:intergenic 60.0%
TCCTGACGGGAACACCGTGT+CGG + tig0018650:66751-66770 Msa1368600:CDS 60.0%
GAACTGTGGTTACGGGACGG+TGG + tig0018650:66781-66800 Msa1368600:CDS 60.0%
GCGGCAGTAGAGAGTAGCGG+TGG - tig0018650:66050-66069 None:intergenic 65.0%
AGAGGAAGGCGGAGTCAGGG+CGG - tig0018650:66069-66088 None:intergenic 65.0%
CGCAGAGGAAGGCGGAGTCA+GGG - tig0018650:66072-66091 None:intergenic 65.0%
CCTGACTCCGCCTTCCTCTG+CGG + tig0018650:66070-66089 Msa1368600:CDS 65.0%
AGTGACGCGAGGGTGACGAG+AGG - tig0018650:66134-66153 None:intergenic 65.0%
GGGAACACGCTCGTGACGTG+CGG - tig0018650:66263-66282 None:intergenic 65.0%
TCCGACACGGTGTTCCCGTC+AGG - tig0018650:66755-66774 None:intergenic 65.0%
CTGTGGTTACGGGACGGTGG+CGG + tig0018650:66784-66803 Msa1368600:CDS 65.0%
TGGCGGCGGATCGTGAAGCT+AGG + tig0018650:66801-66820 Msa1368600:CDS 65.0%
GGCGGCGGATCGTGAAGCTA+GGG + tig0018650:66802-66821 Msa1368600:CDS 65.0%
GCGGCAGTAGAGAGTAGCGG+TGG - tig0018650:66050-66069 None:intergenic 65.0%
AGAGGAAGGCGGAGTCAGGG+CGG - tig0018650:66069-66088 None:intergenic 65.0%
CGCAGAGGAAGGCGGAGTCA+GGG - tig0018650:66072-66091 None:intergenic 65.0%
CCTGACTCCGCCTTCCTCTG+CGG + tig0018650:66070-66089 Msa1368600:CDS 65.0%
AGTGACGCGAGGGTGACGAG+AGG - tig0018650:66134-66153 None:intergenic 65.0%
GGGAACACGCTCGTGACGTG+CGG - tig0018650:66263-66282 None:intergenic 65.0%
TCCGACACGGTGTTCCCGTC+AGG - tig0018650:66755-66774 None:intergenic 65.0%
CTGTGGTTACGGGACGGTGG+CGG + tig0018650:66784-66803 Msa1368600:CDS 65.0%
TGGCGGCGGATCGTGAAGCT+AGG + tig0018650:66801-66820 Msa1368600:CDS 65.0%
GGCGGCGGATCGTGAAGCTA+GGG + tig0018650:66802-66821 Msa1368600:CDS 65.0%
CCGCAGAGGAAGGCGGAGTC+AGG - tig0018650:66073-66092 None:intergenic 70.0%
! ACAGGCGCCGCAGAGGAAGG+CGG - tig0018650:66080-66099 None:intergenic 70.0%
! GTCACAGGCGCCGCAGAGGA+AGG - tig0018650:66083-66102 None:intergenic 70.0%
! TGGAGTCACAGGCGCCGCAG+AGG - tig0018650:66087-66106 None:intergenic 70.0%
! TGGTTACGGGACGGTGGCGG+CGG + tig0018650:66787-66806 Msa1368600:CDS 70.0%
CCGCAGAGGAAGGCGGAGTC+AGG - tig0018650:66073-66092 None:intergenic 70.0%
! ACAGGCGCCGCAGAGGAAGG+CGG - tig0018650:66080-66099 None:intergenic 70.0%
! GTCACAGGCGCCGCAGAGGA+AGG - tig0018650:66083-66102 None:intergenic 70.0%
! TGGAGTCACAGGCGCCGCAG+AGG - tig0018650:66087-66106 None:intergenic 70.0%
! TGGTTACGGGACGGTGGCGG+CGG + tig0018650:66787-66806 Msa1368600:CDS 70.0%
CGCTCGTGACGTGCGGCGAG+AGG - tig0018650:66256-66275 None:intergenic 75.0%
CGCTCGTGACGTGCGGCGAG+AGG - tig0018650:66256-66275 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0018650 gene 65933 67330 65933 ID=Msa1368600;Name=Msa1368600
tig0018650 mRNA 65933 67330 65933 ID=Msa1368600-mRNA-1;Parent=Msa1368600;Name=Msa1368600-mRNA-1;_AED=0.00;_eAED=0.00;_QI=80|1|1|1|1|1|2|339|290
tig0018650 exon 65933 66612 65933 ID=Msa1368600-mRNA-1:exon:518;Parent=Msa1368600-mRNA-1
tig0018650 exon 66719 67330 66719 ID=Msa1368600-mRNA-1:exon:519;Parent=Msa1368600-mRNA-1
tig0018650 five_prime_UTR 65933 66012 65933 ID=Msa1368600-mRNA-1:five_prime_utr;Parent=Msa1368600-mRNA-1
tig0018650 CDS 66013 66612 66013 ID=Msa1368600-mRNA-1:cds;Parent=Msa1368600-mRNA-1
tig0018650 CDS 66719 66991 66719 ID=Msa1368600-mRNA-1:cds;Parent=Msa1368600-mRNA-1
tig0018650 three_prime_UTR 66992 67330 66992 ID=Msa1368600-mRNA-1:three_prime_utr;Parent=Msa1368600-mRNA-1
Gene Sequence

>Msa1368600

ATGGCTTCTAAACTCTGTGACTCATGCAAATCCGCCACCGCTACTCTCTACTGCCGCCCTGACTCCGCCTTCCTCTGCGGCGCCTGTGACTCCAAAGTCCACGCCGCTAACAAACTCGCCTCTCGTCACCCTCGCGTCACTCTCTGCGAAGTCTGCGAACAAGCACCTGCACATGTCACGTGTAAAGCCGATGCAGCTTCTCTCTGCATCACCTGTGACCGTGACATCCACACTGCTAATCCTCTCGCCGCACGTCACGAGCGTGTTCCCGTTACTCCGTTCTTCGAATCCAACACTTCTCACTCTGTTAAATCGCTTCAAAACAATAACAACAACTACGATGCTGTTAAAGATGAAGCTGAAGTTGCTTCGTGGTTGATCTCTGATCCGAAAGCAGATCTCAACTCATCTCCTTATCTTTTCTCTGATTCCGAAGCTATTCCGTTCATGGATCTCGATTACGGTGTAATTGAACATAAAAACGACGGTGTTGTTCCCGTTCATGGCAACTTCGATCCATTTGTTTCAGCTTACAAGAACAACAACGTGCACTTGCATACAGAGTTAGAAACACCGTCACAGTCGCAGATTAGTCAGAGTGTATCGTCTTCGTCGATGGAGGTTGGAGTTGTTCCTGACGGGAACACCGTGTCGGAGATATCGAACTGTGGTTACGGGACGGTGGCGGCGGATCGTGAAGCTAGGGTTATGAGGTATAGGGAGAAGAGGAAGAATAGAAGATTCGAGAAGACGATTCGTTACGCTTCTCGTAAAGCATATGCGGAAACTAGGCCTAGGATTAAAGGGAGGTTTGCAAAACGCACTGATGCAGTTGATTCCATTTCTGGATACGGCATCGTTCCCACTTGTTGA
Protein sequence

>Msa1368600

MASKLCDSCKSATATLYCRPDSAFLCGACDSKVHAANKLASRHPRVTLCEVCEQAPAHVTCKADAASLCITCDRDIHTANPLAARHERVPVTPFFESNTSHSVKSLQNNNNNYDAVKDEAEVASWLISDPKADLNSSPYLFSDSEAIPFMDLDYGVIEHKNDGVVPVHGNFDPFVSAYKNNNVHLHTELETPSQSQISQSVSSSSMEVGVVPDGNTVSEISNCGYGTVAADREARVMRYREKRKNRRFEKTIRYASRKAYAETRPRIKGRFAKRTDAVDSISGYGIVPTC*