AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0520760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0520760 MtrunA17_Chr3g0136701 97.241 290 8 0 1 290 1 290 0.0 588
Msa0520760 MtrunA17_Chr1g0150471 54.403 318 115 7 1 290 1 316 3.78e-106 311
Msa0520760 MtrunA17_Chr4g0072871 51.205 332 101 11 4 280 20 345 3.59e-89 270
Msa0520760 MtrunA17_Chr7g0220721 63.736 91 33 0 3 93 23 113 1.64e-37 137
Msa0520760 MtrunA17_Chr7g0220721 71.429 70 9 2 231 289 326 395 5.35e-23 97.4
Msa0520760 MtrunA17_Chr5g0405151 50.602 83 33 1 5 79 4 86 1.09e-20 87.4
Msa0520760 MtrunA17_Chr5g0429341 43.333 90 51 0 5 94 4 93 2.51e-19 87.4
Msa0520760 MtrunA17_Chr1g0155811 43.299 97 45 1 6 92 5 101 4.94e-19 84.7
Msa0520760 MtrunA17_Chr2g0314671 44.792 96 44 1 6 92 5 100 1.04e-18 84.3
Msa0520760 MtrunA17_Chr4g0033641 43.750 96 45 1 6 92 5 100 1.05e-18 83.2
Msa0520760 MtrunA17_Chr2g0329511 42.708 96 46 1 6 92 5 100 1.56e-17 80.1
Msa0520760 MtrunA17_Chr4g0035651 39.175 97 49 1 6 92 5 101 5.05e-17 78.2
Msa0520760 MtrunA17_Chr3g0145061 38.144 97 50 1 6 92 5 101 6.31e-17 78.2
Msa0520760 MtrunA17_Chr3g0120711 39.423 104 62 1 5 108 4 106 1.79e-16 79.0
Msa0520760 MtrunA17_Chr3g0120711 44.872 78 40 1 202 279 327 401 3.28e-11 63.2
Msa0520760 MtrunA17_Chr7g0268691 39.326 89 53 1 6 94 20 107 3.01e-16 78.2
Msa0520760 MtrunA17_Chr2g0322941 45.122 82 45 0 5 86 43 124 3.21e-15 73.6
Msa0520760 MtrunA17_Chr3g0142221 37.895 95 48 1 6 89 5 99 8.23e-15 72.0
Msa0520760 MtrunA17_Chr7g0251781 44.444 72 40 0 6 77 5 76 1.28e-13 70.5
Msa0520760 MtrunA17_Chr2g0322951 38.318 107 46 2 189 279 37 139 1.26e-12 64.3
Msa0520760 MtrunA17_Chr1g0209071 39.189 74 45 0 4 77 3 76 1.43e-12 67.0
Msa0520760 MtrunA17_Chr1g0209861 61.818 55 17 1 224 274 370 424 3.14e-12 66.6
Msa0520760 MtrunA17_Chr8g0390971 70.455 44 13 0 232 275 358 401 4.74e-12 65.9
Msa0520760 MtrunA17_Chr5g0431351 66.667 45 15 0 230 274 172 216 6.93e-12 63.5
Msa0520760 MtrunA17_Chr3g0127941 49.231 65 32 1 219 283 617 680 7.29e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0520760 AT5G24930.1 52.000 375 80 13 1 289 1 361 4.03e-95 285
Msa0520760 AT2G24790.1 54.608 293 94 11 2 277 4 274 1.40e-94 281
Msa0520760 AT5G57660.1 42.775 346 131 10 2 289 18 354 2.40e-74 232
Msa0520760 AT3G02380.1 40.351 342 141 9 3 289 13 346 3.03e-73 229
Msa0520760 AT5G15850.1 41.210 347 143 8 3 289 9 354 3.56e-70 221
Msa0520760 AT5G15840.1 39.335 361 134 14 6 289 20 372 2.57e-65 209
Msa0520760 AT2G24790.2 56.742 178 54 6 2 171 4 166 2.74e-58 186
Msa0520760 AT5G15840.2 41.935 186 82 7 6 166 20 204 3.84e-37 133
Msa0520760 AT2G47890.1 28.235 340 158 13 4 276 11 331 1.81e-26 106
Msa0520760 AT1G49130.1 28.435 313 176 10 4 280 26 326 6.10e-23 96.7
Msa0520760 AT1G49130.2 28.435 313 176 10 4 280 19 319 6.23e-23 96.7
Msa0520760 AT4G38960.1 36.667 150 71 4 5 138 4 145 1.20e-20 87.4
Msa0520760 AT4G38960.6 36.667 150 71 4 5 138 4 145 1.20e-20 87.4
Msa0520760 AT2G21320.1 49.398 83 34 1 5 79 4 86 6.41e-20 85.1
Msa0520760 AT1G78600.1 42.017 119 56 3 6 111 5 123 3.21e-19 85.9
Msa0520760 AT1G78600.2 42.017 119 56 3 6 111 5 123 3.28e-19 86.3
Msa0520760 AT2G47890.3 42.857 91 50 1 4 94 11 99 4.59e-18 82.8
Msa0520760 AT2G47890.2 42.857 91 50 1 4 94 11 99 5.88e-18 81.3
Msa0520760 AT1G06040.1 43.750 96 45 1 6 92 5 100 7.51e-18 81.3
Msa0520760 AT1G06040.2 43.750 96 45 1 6 92 5 100 9.32e-18 79.3
Msa0520760 AT1G75540.1 40.404 99 47 1 6 92 5 103 1.24e-17 82.0
Msa0520760 AT2G33500.1 42.697 89 51 0 6 94 12 100 2.98e-17 81.6
Msa0520760 AT2G33500.1 52.632 57 27 0 220 276 345 401 1.01e-12 68.2
Msa0520760 AT2G33500.2 42.697 89 51 0 6 94 12 100 3.08e-17 81.6
Msa0520760 AT2G33500.2 52.632 57 27 0 220 276 344 400 1.08e-12 68.2
Msa0520760 AT2G31380.1 34.177 158 87 4 6 153 5 155 1.08e-16 77.8
Msa0520760 AT1G28050.1 41.573 89 52 0 6 94 9 97 4.67e-16 78.2
Msa0520760 AT1G28050.1 56.000 50 22 0 228 277 381 430 9.07e-11 62.4
Msa0520760 AT5G48250.1 35.849 106 66 1 5 108 4 109 7.32e-15 74.3
Msa0520760 AT3G07650.3 45.205 73 40 0 5 77 4 76 3.21e-14 72.4
Msa0520760 AT3G07650.3 35.185 108 65 2 174 279 258 362 9.32e-11 62.4
Msa0520760 AT3G07650.2 45.205 73 40 0 5 77 4 76 3.21e-14 72.4
Msa0520760 AT3G07650.2 35.185 108 65 2 174 279 258 362 9.32e-11 62.4
Msa0520760 AT3G07650.4 45.205 73 40 0 5 77 4 76 3.21e-14 72.4
Msa0520760 AT3G07650.4 35.185 108 65 2 174 279 258 362 9.32e-11 62.4
Msa0520760 AT3G07650.1 45.205 73 40 0 5 77 4 76 3.21e-14 72.4
Msa0520760 AT3G07650.1 35.185 108 65 2 174 279 258 362 9.32e-11 62.4
Msa0520760 AT4G10240.1 33.714 175 81 7 6 165 5 159 4.74e-14 68.9
Msa0520760 AT4G39070.1 37.755 98 51 1 5 92 4 101 1.61e-13 69.3
Msa0520760 AT1G73870.1 55.556 63 27 1 213 275 327 388 2.49e-13 70.1
Msa0520760 AT4G15250.1 40.476 84 50 0 6 89 5 88 4.66e-13 68.9
Msa0520760 AT1G68190.2 38.824 85 50 1 4 88 41 123 6.42e-13 68.9
Msa0520760 AT1G68190.1 38.824 85 50 1 4 88 12 94 7.37e-13 68.6
Msa0520760 AT1G68190.3 38.824 85 50 1 4 88 12 94 7.37e-13 68.6
Msa0520760 AT4G38960.3 28.497 193 71 5 5 138 4 188 1.01e-12 66.6
Msa0520760 AT1G25440.1 36.029 136 72 5 158 286 288 415 5.02e-12 66.2
Msa0520760 AT3G21880.1 38.202 89 53 1 6 94 5 91 1.20e-11 65.1
Msa0520760 AT3G21880.3 38.202 89 53 1 6 94 9 95 1.38e-11 64.7
Msa0520760 AT3G21880.2 38.202 89 53 1 6 94 9 95 1.38e-11 64.7
Msa0520760 AT5G14370.1 63.043 46 17 0 232 277 294 339 1.92e-11 64.3
Msa0520760 AT4G25990.1 60.870 46 18 0 232 277 341 386 2.35e-11 64.3
Msa0520760 AT1G68520.1 68.182 44 14 0 232 275 357 400 2.51e-11 63.9
Msa0520760 AT5G60100.2 31.217 189 106 6 95 276 342 513 3.49e-11 63.9
Msa0520760 AT5G60100.5 31.217 189 106 6 95 276 315 486 4.46e-11 63.5
Msa0520760 AT5G60100.3 31.217 189 106 6 95 276 315 486 4.46e-11 63.5
Msa0520760 AT5G60100.1 31.217 189 106 6 95 276 315 486 4.46e-11 63.5

Find 80 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCAGTTGATTCCATTTC+TGG 0.107062 3_4:+73291570 Msa0520760:CDS
TCTTGCTTGCCAAACGATTT+TGG 0.181015 3_4:+73291745 Msa0520760:three_prime_UTR
ACAGAGTTTAGAAGCCATTT+TGG 0.192107 3_4:-73290634 None:intergenic
CAACATGCAAATCTCCAAAA+AGG 0.239203 3_4:-73291830 None:intergenic
GTAATAATGTTATGGTAAAA+AGG 0.244996 3_4:+73291778 Msa0520760:three_prime_UTR
GAAACTAGGCCTAGGATTAA+AGG 0.280004 3_4:+73291528 Msa0520760:CDS
AAACAAACTTCATTCCAAAA+TGG 0.295788 3_4:+73290620 Msa0520760:exon
CCGCAGAGGAAGGCGGAGTC+AGG 0.296878 3_4:-73290696 None:intergenic
TTCCGAAGCTATTCCGTTCA+TGG 0.298068 3_4:+73291067 Msa0520760:CDS
AAGCTAGGGTTATGAGGTAT+AGG 0.350898 3_4:+73291442 Msa0520760:CDS
GACGGTGTTGTTCCCGTTCA+TGG 0.369225 3_4:+73291122 Msa0520760:CDS
GCAAACCTCCCTTTAATCCT+AGG 0.392261 3_4:-73291537 None:intergenic
CCGTTCATGGATCTCGATTA+CGG 0.395344 3_4:+73291080 Msa0520760:CDS
TTTGTTAGCGGCGTGGACTT+TGG 0.416071 3_4:-73290730 None:intergenic
GGATCGAAGTTGCCATGAAC+GGG 0.430806 3_4:-73291134 None:intergenic
TGGCGGCGGATCGTGAAGCT+AGG 0.432649 3_4:+73291427 Msa0520760:CDS
GATTCGAAGAACGGAGTAAC+GGG 0.439450 3_4:-73290906 None:intergenic
AGCTAGGGTTATGAGGTATA+GGG 0.439658 3_4:+73291443 Msa0520760:CDS
AAATTCACCCCAAAATCGTT+TGG 0.440965 3_4:-73291754 None:intergenic
AGATATCGAACTGTGGTTAC+GGG 0.444139 3_4:+73291400 Msa0520760:CDS
GAGATATCGAACTGTGGTTA+CGG 0.445942 3_4:+73291399 Msa0520760:CDS
AACGATGCCGTATCCAGAAA+TGG 0.448783 3_4:-73291583 None:intergenic
GTTGATTCCATTTCTGGATA+CGG 0.451790 3_4:+73291576 Msa0520760:CDS
GATGAGTTGAGATCTGCTTT+CGG 0.461772 3_4:-73291026 None:intergenic
AGAAGCGTTGTTATGTGAAA+AGG 0.461814 3_4:-73290571 None:intergenic
AGGGTCTGAAAGACTGCAAC+CGG 0.468857 3_4:-73291810 None:intergenic
TCCGACACGGTGTTCCCGTC+AGG 0.479338 3_4:-73291378 None:intergenic
TTTAACAGAGTGAGAAGTGT+TGG 0.480554 3_4:-73290928 None:intergenic
TGGTTACGGGACGGTGGCGG+CGG 0.488159 3_4:+73291413 Msa0520760:CDS
AAACTAGGCCTAGGATTAAA+GGG 0.490422 3_4:+73291529 Msa0520760:CDS
AGTGTGGATGTCACGGTCAC+AGG 0.492186 3_4:-73290850 None:intergenic
ATCCATGAACGGAATAGCTT+CGG 0.492715 3_4:-73291069 None:intergenic
TTATGAGGTATAGGGAGAAG+AGG 0.496368 3_4:+73291451 Msa0520760:CDS
TTCTTGTAAGCTGAAACAAA+TGG 0.496560 3_4:-73291155 None:intergenic
TTAGGTATCGTCTTCGTCGA+TGG 0.503016 3_4:+73291341 Msa0520760:intron
ATCTGTAATAACATTCGCTT+AGG 0.508607 3_4:+73291867 Msa0520760:three_prime_UTR
GTAAAGCATATGCGGAAACT+AGG 0.510714 3_4:+73291514 Msa0520760:CDS
CTGTGGTTACGGGACGGTGG+CGG 0.513006 3_4:+73291410 Msa0520760:CDS
CGCAGAGGAAGGCGGAGTCA+GGG 0.518288 3_4:-73290695 None:intergenic
TCGTCTTCGTCGATGGAGGT+TGG 0.523017 3_4:+73291348 Msa0520760:CDS
AGGTTGGAGTTGTTCCTGAC+GGG 0.524585 3_4:+73291364 Msa0520760:CDS
GGATTCGAAGAACGGAGTAA+CGG 0.536432 3_4:-73290907 None:intergenic
GCTTTACACGTGACATGTGC+AGG 0.538227 3_4:-73290804 None:intergenic
GGCGTGGACTTTGGAGTCAC+AGG 0.543388 3_4:-73290721 None:intergenic
GTCACAGGCGCCGCAGAGGA+AGG 0.545693 3_4:-73290706 None:intergenic
TCGGAATCAGAGAAAAGATA+AGG 0.547155 3_4:-73291050 None:intergenic
TGGATCGAAGTTGCCATGAA+CGG 0.558189 3_4:-73291135 None:intergenic
CGCTCGTGACGTGCGGCGAG+AGG 0.558417 3_4:-73290879 None:intergenic
ATGAAGCTGAAGTTGCTTCG+TGG 0.559796 3_4:+73290991 Msa0520760:CDS
GCAGTAGAGAGTAGCGGTGG+CGG 0.563031 3_4:-73290670 None:intergenic
GAAGTGTTGGATTCGAAGAA+CGG 0.565969 3_4:-73290915 None:intergenic
GATGCAGAGAGAAGCTGCAT+CGG 0.576026 3_4:-73290826 None:intergenic
CATATGCGGAAACTAGGCCT+AGG 0.577094 3_4:+73291520 Msa0520760:CDS
GGCGGCGGATCGTGAAGCTA+GGG 0.577274 3_4:+73291428 Msa0520760:CDS
ACGAGAGGCGAGTTTGTTAG+CGG 0.583367 3_4:-73290742 None:intergenic
GGTATCGTCTTCGTCGATGG+AGG 0.585063 3_4:+73291344 Msa0520760:intron
ACAGGCGCCGCAGAGGAAGG+CGG 0.586506 3_4:-73290703 None:intergenic
GATTAGCAGTGTGGATGTCA+CGG 0.589251 3_4:-73290857 None:intergenic
CGCTTCTCGTAAAGCATATG+CGG 0.592961 3_4:+73291506 Msa0520760:CDS
GAGGTTGGAGTTGTTCCTGA+CGG 0.607148 3_4:+73291363 Msa0520760:CDS
GGGAACACGCTCGTGACGTG+CGG 0.610507 3_4:-73290886 None:intergenic
ATCGTGAAGCTAGGGTTATG+AGG 0.615678 3_4:+73291436 Msa0520760:CDS
TGGAGTCACAGGCGCCGCAG+AGG 0.616423 3_4:-73290710 None:intergenic
AGGCGAGTTTGTTAGCGGCG+TGG 0.630051 3_4:-73290737 None:intergenic
TGACTAATCTGCGACTGTGA+CGG 0.630286 3_4:-73291212 None:intergenic
AGTGACGCGAGGGTGACGAG+AGG 0.632213 3_4:-73290757 None:intergenic
GCGGCAGTAGAGAGTAGCGG+TGG 0.638763 3_4:-73290673 None:intergenic
AGGGCGGCAGTAGAGAGTAG+CGG 0.646899 3_4:-73290676 None:intergenic
CGGCGAGAGGATTAGCAGTG+TGG 0.659805 3_4:-73290866 None:intergenic
AGAGGAAGGCGGAGTCAGGG+CGG 0.663446 3_4:-73290692 None:intergenic
CCTGACTCCGCCTTCCTCTG+CGG 0.671751 3_4:+73290696 Msa0520760:CDS
ATCGAACTGTGGTTACGGGA+CGG 0.672790 3_4:+73291404 Msa0520760:CDS
TCCTGACGGGAACACCGTGT+CGG 0.682213 3_4:+73291377 Msa0520760:CDS
GAACTGTGGTTACGGGACGG+TGG 0.687113 3_4:+73291407 Msa0520760:CDS
CTTCGCAGAGAGTGACGCGA+GGG 0.694593 3_4:-73290767 None:intergenic
CTAGGCCTAGGATTAAAGGG+AGG 0.700310 3_4:+73291532 Msa0520760:CDS
ACTTCGCAGAGAGTGACGCG+AGG 0.710854 3_4:-73290768 None:intergenic
CCGTAATCGAGATCCATGAA+CGG 0.730276 3_4:-73291080 None:intergenic
ACAGTTCGATATCTCCGACA+CGG 0.744977 3_4:-73291391 None:intergenic
GTGTCGGAGATATCGAACTG+TGG 0.760977 3_4:+73291393 Msa0520760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATATTATAGTAATCAA+AGG + chr3_4:73291925-73291944 Msa0520760:three_prime_UTR 10.0%
!!! TTTTTTTGTTGTGATTAATT+AGG + chr3_4:73291323-73291342 Msa0520760:intron 15.0%
!!! TGAATTTTGTAATAATGTTA+TGG + chr3_4:73291770-73291789 Msa0520760:three_prime_UTR 15.0%
!!! TTTTTTTGTTGTGATTAATT+AGG + chr3_4:73291323-73291342 Msa0520760:intron 15.0%
!!! TGAATTTTGTAATAATGTTA+TGG + chr3_4:73291770-73291789 Msa0520760:three_prime_UTR 15.0%
!!! TAAGTTTGTTTTTATTGACA+TGG + chr3_4:73291264-73291283 Msa0520760:intron 20.0%
!! GTAATAATGTTATGGTAAAA+AGG + chr3_4:73291778-73291797 Msa0520760:three_prime_UTR 20.0%
!!! TAAGTTTGTTTTTATTGACA+TGG + chr3_4:73291264-73291283 Msa0520760:intron 20.0%
!! GTAATAATGTTATGGTAAAA+AGG + chr3_4:73291778-73291797 Msa0520760:three_prime_UTR 20.0%
!!! ATTTTGGAATGAAGTTTGTT+TGG - chr3_4:73290621-73290640 None:intergenic 25.0%
! AAACAAACTTCATTCCAAAA+TGG + chr3_4:73290620-73290639 Msa0520760:exon 25.0%
! GTAATTGAACATAAAAACGA+CGG + chr3_4:73291104-73291123 Msa0520760:CDS 25.0%
! CAAAAAAGAAATCAACAAGT+GGG - chr3_4:73291609-73291628 None:intergenic 25.0%
!!! ATTTTGGAATGAAGTTTGTT+TGG - chr3_4:73290621-73290640 None:intergenic 25.0%
! AAACAAACTTCATTCCAAAA+TGG + chr3_4:73290620-73290639 Msa0520760:exon 25.0%
! GTAATTGAACATAAAAACGA+CGG + chr3_4:73291104-73291123 Msa0520760:CDS 25.0%
! CAAAAAAGAAATCAACAAGT+GGG - chr3_4:73291609-73291628 None:intergenic 25.0%
TTCTTGTAAGCTGAAACAAA+TGG - chr3_4:73291158-73291177 None:intergenic 30.0%
GCAAAAAAGAAATCAACAAG+TGG - chr3_4:73291610-73291629 None:intergenic 30.0%
AACATGCAAATCTCCAAAAA+GGG - chr3_4:73291832-73291851 None:intergenic 30.0%
ATCTGTAATAACATTCGCTT+AGG + chr3_4:73291867-73291886 Msa0520760:three_prime_UTR 30.0%
TTCTTGTAAGCTGAAACAAA+TGG - chr3_4:73291158-73291177 None:intergenic 30.0%
GCAAAAAAGAAATCAACAAG+TGG - chr3_4:73291610-73291629 None:intergenic 30.0%
AACATGCAAATCTCCAAAAA+GGG - chr3_4:73291832-73291851 None:intergenic 30.0%
ATCTGTAATAACATTCGCTT+AGG + chr3_4:73291867-73291886 Msa0520760:three_prime_UTR 30.0%
!! AGAAGCGTTGTTATGTGAAA+AGG - chr3_4:73290574-73290593 None:intergenic 35.0%
! ACAGAGTTTAGAAGCCATTT+TGG - chr3_4:73290637-73290656 None:intergenic 35.0%
! TTTAACAGAGTGAGAAGTGT+TGG - chr3_4:73290931-73290950 None:intergenic 35.0%
TCGGAATCAGAGAAAAGATA+AGG - chr3_4:73291053-73291072 None:intergenic 35.0%
AAACTAGGCCTAGGATTAAA+GGG + chr3_4:73291529-73291548 Msa0520760:CDS 35.0%
! GTTGATTCCATTTCTGGATA+CGG + chr3_4:73291576-73291595 Msa0520760:CDS 35.0%
AAATTCACCCCAAAATCGTT+TGG - chr3_4:73291757-73291776 None:intergenic 35.0%
GGTAAAAAGGAAGATGATAC+CGG + chr3_4:73291791-73291810 Msa0520760:three_prime_UTR 35.0%
CAACATGCAAATCTCCAAAA+AGG - chr3_4:73291833-73291852 None:intergenic 35.0%
!! AGAAGCGTTGTTATGTGAAA+AGG - chr3_4:73290574-73290593 None:intergenic 35.0%
! ACAGAGTTTAGAAGCCATTT+TGG - chr3_4:73290637-73290656 None:intergenic 35.0%
! TTTAACAGAGTGAGAAGTGT+TGG - chr3_4:73290931-73290950 None:intergenic 35.0%
TCGGAATCAGAGAAAAGATA+AGG - chr3_4:73291053-73291072 None:intergenic 35.0%
AAACTAGGCCTAGGATTAAA+GGG + chr3_4:73291529-73291548 Msa0520760:CDS 35.0%
! GTTGATTCCATTTCTGGATA+CGG + chr3_4:73291576-73291595 Msa0520760:CDS 35.0%
AAATTCACCCCAAAATCGTT+TGG - chr3_4:73291757-73291776 None:intergenic 35.0%
GGTAAAAAGGAAGATGATAC+CGG + chr3_4:73291791-73291810 Msa0520760:three_prime_UTR 35.0%
CAACATGCAAATCTCCAAAA+AGG - chr3_4:73291833-73291852 None:intergenic 35.0%
!! GAAGTGTTGGATTCGAAGAA+CGG - chr3_4:73290918-73290937 None:intergenic 40.0%
! GATGAGTTGAGATCTGCTTT+CGG - chr3_4:73291029-73291048 None:intergenic 40.0%
ATCCATGAACGGAATAGCTT+CGG - chr3_4:73291072-73291091 None:intergenic 40.0%
GAGATATCGAACTGTGGTTA+CGG + chr3_4:73291399-73291418 Msa0520760:CDS 40.0%
AGATATCGAACTGTGGTTAC+GGG + chr3_4:73291400-73291419 Msa0520760:CDS 40.0%
AAGCTAGGGTTATGAGGTAT+AGG + chr3_4:73291442-73291461 Msa0520760:CDS 40.0%
AGCTAGGGTTATGAGGTATA+GGG + chr3_4:73291443-73291462 Msa0520760:CDS 40.0%
TTATGAGGTATAGGGAGAAG+AGG + chr3_4:73291451-73291470 Msa0520760:CDS 40.0%
GTAAAGCATATGCGGAAACT+AGG + chr3_4:73291514-73291533 Msa0520760:CDS 40.0%
GAAACTAGGCCTAGGATTAA+AGG + chr3_4:73291528-73291547 Msa0520760:CDS 40.0%
! GATGCAGTTGATTCCATTTC+TGG + chr3_4:73291570-73291589 Msa0520760:CDS 40.0%
! TCTTGCTTGCCAAACGATTT+TGG + chr3_4:73291745-73291764 Msa0520760:three_prime_UTR 40.0%
! CTTGCTTGCCAAACGATTTT+GGG + chr3_4:73291746-73291765 Msa0520760:three_prime_UTR 40.0%
! TTGCTTGCCAAACGATTTTG+GGG + chr3_4:73291747-73291766 Msa0520760:three_prime_UTR 40.0%
!! GAAGTGTTGGATTCGAAGAA+CGG - chr3_4:73290918-73290937 None:intergenic 40.0%
! GATGAGTTGAGATCTGCTTT+CGG - chr3_4:73291029-73291048 None:intergenic 40.0%
ATCCATGAACGGAATAGCTT+CGG - chr3_4:73291072-73291091 None:intergenic 40.0%
GAGATATCGAACTGTGGTTA+CGG + chr3_4:73291399-73291418 Msa0520760:CDS 40.0%
AGATATCGAACTGTGGTTAC+GGG + chr3_4:73291400-73291419 Msa0520760:CDS 40.0%
AAGCTAGGGTTATGAGGTAT+AGG + chr3_4:73291442-73291461 Msa0520760:CDS 40.0%
AGCTAGGGTTATGAGGTATA+GGG + chr3_4:73291443-73291462 Msa0520760:CDS 40.0%
TTATGAGGTATAGGGAGAAG+AGG + chr3_4:73291451-73291470 Msa0520760:CDS 40.0%
GTAAAGCATATGCGGAAACT+AGG + chr3_4:73291514-73291533 Msa0520760:CDS 40.0%
GAAACTAGGCCTAGGATTAA+AGG + chr3_4:73291528-73291547 Msa0520760:CDS 40.0%
! GATGCAGTTGATTCCATTTC+TGG + chr3_4:73291570-73291589 Msa0520760:CDS 40.0%
! TCTTGCTTGCCAAACGATTT+TGG + chr3_4:73291745-73291764 Msa0520760:three_prime_UTR 40.0%
! CTTGCTTGCCAAACGATTTT+GGG + chr3_4:73291746-73291765 Msa0520760:three_prime_UTR 40.0%
! TTGCTTGCCAAACGATTTTG+GGG + chr3_4:73291747-73291766 Msa0520760:three_prime_UTR 40.0%
GATTAGCAGTGTGGATGTCA+CGG - chr3_4:73290860-73290879 None:intergenic 45.0%
GATTCGAAGAACGGAGTAAC+GGG - chr3_4:73290909-73290928 None:intergenic 45.0%
GGATTCGAAGAACGGAGTAA+CGG - chr3_4:73290910-73290929 None:intergenic 45.0%
ATGAAGCTGAAGTTGCTTCG+TGG + chr3_4:73290991-73291010 Msa0520760:CDS 45.0%
TTCCGAAGCTATTCCGTTCA+TGG + chr3_4:73291067-73291086 Msa0520760:CDS 45.0%
CCGTAATCGAGATCCATGAA+CGG - chr3_4:73291083-73291102 None:intergenic 45.0%
CCGTTCATGGATCTCGATTA+CGG + chr3_4:73291080-73291099 Msa0520760:CDS 45.0%
TGGATCGAAGTTGCCATGAA+CGG - chr3_4:73291138-73291157 None:intergenic 45.0%
TGACTAATCTGCGACTGTGA+CGG - chr3_4:73291215-73291234 None:intergenic 45.0%
! TTAGGTATCGTCTTCGTCGA+TGG + chr3_4:73291341-73291360 Msa0520760:intron 45.0%
ACAGTTCGATATCTCCGACA+CGG - chr3_4:73291394-73291413 None:intergenic 45.0%
ATCGTGAAGCTAGGGTTATG+AGG + chr3_4:73291436-73291455 Msa0520760:CDS 45.0%
CGCTTCTCGTAAAGCATATG+CGG + chr3_4:73291506-73291525 Msa0520760:CDS 45.0%
GCAAACCTCCCTTTAATCCT+AGG - chr3_4:73291540-73291559 None:intergenic 45.0%
AACGATGCCGTATCCAGAAA+TGG - chr3_4:73291586-73291605 None:intergenic 45.0%
! GCAGTCTTTCAGACCCTTTT+TGG + chr3_4:73291816-73291835 Msa0520760:three_prime_UTR 45.0%
GATTAGCAGTGTGGATGTCA+CGG - chr3_4:73290860-73290879 None:intergenic 45.0%
GATTCGAAGAACGGAGTAAC+GGG - chr3_4:73290909-73290928 None:intergenic 45.0%
GGATTCGAAGAACGGAGTAA+CGG - chr3_4:73290910-73290929 None:intergenic 45.0%
ATGAAGCTGAAGTTGCTTCG+TGG + chr3_4:73290991-73291010 Msa0520760:CDS 45.0%
TTCCGAAGCTATTCCGTTCA+TGG + chr3_4:73291067-73291086 Msa0520760:CDS 45.0%
CCGTAATCGAGATCCATGAA+CGG - chr3_4:73291083-73291102 None:intergenic 45.0%
CCGTTCATGGATCTCGATTA+CGG + chr3_4:73291080-73291099 Msa0520760:CDS 45.0%
TGGATCGAAGTTGCCATGAA+CGG - chr3_4:73291138-73291157 None:intergenic 45.0%
TGACTAATCTGCGACTGTGA+CGG - chr3_4:73291215-73291234 None:intergenic 45.0%
! TTAGGTATCGTCTTCGTCGA+TGG + chr3_4:73291341-73291360 Msa0520760:intron 45.0%
ACAGTTCGATATCTCCGACA+CGG - chr3_4:73291394-73291413 None:intergenic 45.0%
ATCGTGAAGCTAGGGTTATG+AGG + chr3_4:73291436-73291455 Msa0520760:CDS 45.0%
CGCTTCTCGTAAAGCATATG+CGG + chr3_4:73291506-73291525 Msa0520760:CDS 45.0%
GCAAACCTCCCTTTAATCCT+AGG - chr3_4:73291540-73291559 None:intergenic 45.0%
AACGATGCCGTATCCAGAAA+TGG - chr3_4:73291586-73291605 None:intergenic 45.0%
! GCAGTCTTTCAGACCCTTTT+TGG + chr3_4:73291816-73291835 Msa0520760:three_prime_UTR 45.0%
TTTGTTAGCGGCGTGGACTT+TGG - chr3_4:73290733-73290752 None:intergenic 50.0%
!! ACGAGAGGCGAGTTTGTTAG+CGG - chr3_4:73290745-73290764 None:intergenic 50.0%
GCTTTACACGTGACATGTGC+AGG - chr3_4:73290807-73290826 None:intergenic 50.0%
GATGCAGAGAGAAGCTGCAT+CGG - chr3_4:73290829-73290848 None:intergenic 50.0%
GGATCGAAGTTGCCATGAAC+GGG - chr3_4:73291137-73291156 None:intergenic 50.0%
GAGGTTGGAGTTGTTCCTGA+CGG + chr3_4:73291363-73291382 Msa0520760:CDS 50.0%
AGGTTGGAGTTGTTCCTGAC+GGG + chr3_4:73291364-73291383 Msa0520760:CDS 50.0%
GTGTCGGAGATATCGAACTG+TGG + chr3_4:73291393-73291412 Msa0520760:CDS 50.0%
ATCGAACTGTGGTTACGGGA+CGG + chr3_4:73291404-73291423 Msa0520760:CDS 50.0%
CATATGCGGAAACTAGGCCT+AGG + chr3_4:73291520-73291539 Msa0520760:CDS 50.0%
CTAGGCCTAGGATTAAAGGG+AGG + chr3_4:73291532-73291551 Msa0520760:CDS 50.0%
AGGGTCTGAAAGACTGCAAC+CGG - chr3_4:73291813-73291832 None:intergenic 50.0%
TTTGTTAGCGGCGTGGACTT+TGG - chr3_4:73290733-73290752 None:intergenic 50.0%
!! ACGAGAGGCGAGTTTGTTAG+CGG - chr3_4:73290745-73290764 None:intergenic 50.0%
GCTTTACACGTGACATGTGC+AGG - chr3_4:73290807-73290826 None:intergenic 50.0%
GATGCAGAGAGAAGCTGCAT+CGG - chr3_4:73290829-73290848 None:intergenic 50.0%
GGATCGAAGTTGCCATGAAC+GGG - chr3_4:73291137-73291156 None:intergenic 50.0%
GAGGTTGGAGTTGTTCCTGA+CGG + chr3_4:73291363-73291382 Msa0520760:CDS 50.0%
AGGTTGGAGTTGTTCCTGAC+GGG + chr3_4:73291364-73291383 Msa0520760:CDS 50.0%
GTGTCGGAGATATCGAACTG+TGG + chr3_4:73291393-73291412 Msa0520760:CDS 50.0%
ATCGAACTGTGGTTACGGGA+CGG + chr3_4:73291404-73291423 Msa0520760:CDS 50.0%
CATATGCGGAAACTAGGCCT+AGG + chr3_4:73291520-73291539 Msa0520760:CDS 50.0%
CTAGGCCTAGGATTAAAGGG+AGG + chr3_4:73291532-73291551 Msa0520760:CDS 50.0%
AGGGTCTGAAAGACTGCAAC+CGG - chr3_4:73291813-73291832 None:intergenic 50.0%
AGTGTGGATGTCACGGTCAC+AGG - chr3_4:73290853-73290872 None:intergenic 55.0%
!! GACGGTGTTGTTCCCGTTCA+TGG + chr3_4:73291122-73291141 Msa0520760:CDS 55.0%
GGTATCGTCTTCGTCGATGG+AGG + chr3_4:73291344-73291363 Msa0520760:intron 55.0%
TCGTCTTCGTCGATGGAGGT+TGG + chr3_4:73291348-73291367 Msa0520760:CDS 55.0%
AGTGTGGATGTCACGGTCAC+AGG - chr3_4:73290853-73290872 None:intergenic 55.0%
!! GACGGTGTTGTTCCCGTTCA+TGG + chr3_4:73291122-73291141 Msa0520760:CDS 55.0%
GGTATCGTCTTCGTCGATGG+AGG + chr3_4:73291344-73291363 Msa0520760:intron 55.0%
TCGTCTTCGTCGATGGAGGT+TGG + chr3_4:73291348-73291367 Msa0520760:CDS 55.0%
GCAGTAGAGAGTAGCGGTGG+CGG - chr3_4:73290673-73290692 None:intergenic 60.0%
AGGGCGGCAGTAGAGAGTAG+CGG - chr3_4:73290679-73290698 None:intergenic 60.0%
!! GGCGTGGACTTTGGAGTCAC+AGG - chr3_4:73290724-73290743 None:intergenic 60.0%
!! AGGCGAGTTTGTTAGCGGCG+TGG - chr3_4:73290740-73290759 None:intergenic 60.0%
CTTCGCAGAGAGTGACGCGA+GGG - chr3_4:73290770-73290789 None:intergenic 60.0%
ACTTCGCAGAGAGTGACGCG+AGG - chr3_4:73290771-73290790 None:intergenic 60.0%
! CGGCGAGAGGATTAGCAGTG+TGG - chr3_4:73290869-73290888 None:intergenic 60.0%
TCCTGACGGGAACACCGTGT+CGG + chr3_4:73291377-73291396 Msa0520760:CDS 60.0%
GAACTGTGGTTACGGGACGG+TGG + chr3_4:73291407-73291426 Msa0520760:CDS 60.0%
GCAGTAGAGAGTAGCGGTGG+CGG - chr3_4:73290673-73290692 None:intergenic 60.0%
AGGGCGGCAGTAGAGAGTAG+CGG - chr3_4:73290679-73290698 None:intergenic 60.0%
!! GGCGTGGACTTTGGAGTCAC+AGG - chr3_4:73290724-73290743 None:intergenic 60.0%
!! AGGCGAGTTTGTTAGCGGCG+TGG - chr3_4:73290740-73290759 None:intergenic 60.0%
CTTCGCAGAGAGTGACGCGA+GGG - chr3_4:73290770-73290789 None:intergenic 60.0%
ACTTCGCAGAGAGTGACGCG+AGG - chr3_4:73290771-73290790 None:intergenic 60.0%
! CGGCGAGAGGATTAGCAGTG+TGG - chr3_4:73290869-73290888 None:intergenic 60.0%
TCCTGACGGGAACACCGTGT+CGG + chr3_4:73291377-73291396 Msa0520760:CDS 60.0%
GAACTGTGGTTACGGGACGG+TGG + chr3_4:73291407-73291426 Msa0520760:CDS 60.0%
GCGGCAGTAGAGAGTAGCGG+TGG - chr3_4:73290676-73290695 None:intergenic 65.0%
AGAGGAAGGCGGAGTCAGGG+CGG - chr3_4:73290695-73290714 None:intergenic 65.0%
CGCAGAGGAAGGCGGAGTCA+GGG - chr3_4:73290698-73290717 None:intergenic 65.0%
CCTGACTCCGCCTTCCTCTG+CGG + chr3_4:73290696-73290715 Msa0520760:CDS 65.0%
AGTGACGCGAGGGTGACGAG+AGG - chr3_4:73290760-73290779 None:intergenic 65.0%
GGGAACACGCTCGTGACGTG+CGG - chr3_4:73290889-73290908 None:intergenic 65.0%
TCCGACACGGTGTTCCCGTC+AGG - chr3_4:73291381-73291400 None:intergenic 65.0%
CTGTGGTTACGGGACGGTGG+CGG + chr3_4:73291410-73291429 Msa0520760:CDS 65.0%
TGGCGGCGGATCGTGAAGCT+AGG + chr3_4:73291427-73291446 Msa0520760:CDS 65.0%
GGCGGCGGATCGTGAAGCTA+GGG + chr3_4:73291428-73291447 Msa0520760:CDS 65.0%
GCGGCAGTAGAGAGTAGCGG+TGG - chr3_4:73290676-73290695 None:intergenic 65.0%
AGAGGAAGGCGGAGTCAGGG+CGG - chr3_4:73290695-73290714 None:intergenic 65.0%
CGCAGAGGAAGGCGGAGTCA+GGG - chr3_4:73290698-73290717 None:intergenic 65.0%
CCTGACTCCGCCTTCCTCTG+CGG + chr3_4:73290696-73290715 Msa0520760:CDS 65.0%
AGTGACGCGAGGGTGACGAG+AGG - chr3_4:73290760-73290779 None:intergenic 65.0%
GGGAACACGCTCGTGACGTG+CGG - chr3_4:73290889-73290908 None:intergenic 65.0%
TCCGACACGGTGTTCCCGTC+AGG - chr3_4:73291381-73291400 None:intergenic 65.0%
CTGTGGTTACGGGACGGTGG+CGG + chr3_4:73291410-73291429 Msa0520760:CDS 65.0%
TGGCGGCGGATCGTGAAGCT+AGG + chr3_4:73291427-73291446 Msa0520760:CDS 65.0%
GGCGGCGGATCGTGAAGCTA+GGG + chr3_4:73291428-73291447 Msa0520760:CDS 65.0%
CCGCAGAGGAAGGCGGAGTC+AGG - chr3_4:73290699-73290718 None:intergenic 70.0%
! ACAGGCGCCGCAGAGGAAGG+CGG - chr3_4:73290706-73290725 None:intergenic 70.0%
! GTCACAGGCGCCGCAGAGGA+AGG - chr3_4:73290709-73290728 None:intergenic 70.0%
! TGGAGTCACAGGCGCCGCAG+AGG - chr3_4:73290713-73290732 None:intergenic 70.0%
! TGGTTACGGGACGGTGGCGG+CGG + chr3_4:73291413-73291432 Msa0520760:CDS 70.0%
CCGCAGAGGAAGGCGGAGTC+AGG - chr3_4:73290699-73290718 None:intergenic 70.0%
! ACAGGCGCCGCAGAGGAAGG+CGG - chr3_4:73290706-73290725 None:intergenic 70.0%
! GTCACAGGCGCCGCAGAGGA+AGG - chr3_4:73290709-73290728 None:intergenic 70.0%
! TGGAGTCACAGGCGCCGCAG+AGG - chr3_4:73290713-73290732 None:intergenic 70.0%
! TGGTTACGGGACGGTGGCGG+CGG + chr3_4:73291413-73291432 Msa0520760:CDS 70.0%
CGCTCGTGACGTGCGGCGAG+AGG - chr3_4:73290882-73290901 None:intergenic 75.0%
CGCTCGTGACGTGCGGCGAG+AGG - chr3_4:73290882-73290901 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 73290556 73291946 73290556 ID=Msa0520760;Name=Msa0520760
chr3_4 mRNA 73290556 73291946 73290556 ID=Msa0520760-mRNA-1;Parent=Msa0520760;Name=Msa0520760-mRNA-1;_AED=0.00;_eAED=0.00;_QI=83|1|1|1|1|1|2|329|290
chr3_4 exon 73290556 73291238 73290556 ID=Msa0520760-mRNA-1:exon:20532;Parent=Msa0520760-mRNA-1
chr3_4 exon 73291345 73291946 73291345 ID=Msa0520760-mRNA-1:exon:20533;Parent=Msa0520760-mRNA-1
chr3_4 five_prime_UTR 73290556 73290638 73290556 ID=Msa0520760-mRNA-1:five_prime_utr;Parent=Msa0520760-mRNA-1
chr3_4 CDS 73290639 73291238 73290639 ID=Msa0520760-mRNA-1:cds;Parent=Msa0520760-mRNA-1
chr3_4 CDS 73291345 73291617 73291345 ID=Msa0520760-mRNA-1:cds;Parent=Msa0520760-mRNA-1
chr3_4 three_prime_UTR 73291618 73291946 73291618 ID=Msa0520760-mRNA-1:three_prime_utr;Parent=Msa0520760-mRNA-1
Gene Sequence

>Msa0520760

ATGGCTTCTAAACTCTGTGACTCATGCAAATCCGCCACCGCTACTCTCTACTGCCGCCCTGACTCCGCCTTCCTCTGCGGCGCCTGTGACTCCAAAGTCCACGCCGCTAACAAACTCGCCTCTCGTCACCCTCGCGTCACTCTCTGCGAAGTCTGCGAACAAGCACCTGCACATGTCACGTGTAAAGCCGATGCAGCTTCTCTCTGCATCACCTGTGACCGTGACATCCACACTGCTAATCCTCTCGCCGCACGTCACGAGCGTGTTCCCGTTACTCCGTTCTTCGAATCCAACACTTCTCACTCTGTTAAATCGCTTCAAAGCAATAACAACAACTACGATGCTGTTAAAGATGAAGCTGAAGTTGCTTCGTGGTTGATCTCTGATCCGAAAGCAGATCTCAACTCATCTCCTTATCTTTTCTCTGATTCCGAAGCTATTCCGTTCATGGATCTCGATTACGGTGTAATTGAACATAAAAACGACGGTGTTGTTCCCGTTCATGGCAACTTCGATCCATTTGTTTCAGCTTACAAGAACAACAACGTGCACTTGCATACAGAGTTAGAAACACCGTCACAGTCGCAGATTAGTCAGAGTGTATCGTCTTCGTCGATGGAGGTTGGAGTTGTTCCTGACGGGAACACCGTGTCGGAGATATCGAACTGTGGTTACGGGACGGTGGCGGCGGATCGTGAAGCTAGGGTTATGAGGTATAGGGAGAAGAGGAAGAATAGAAGATTCGAGAAGACGATTCGTTACGCTTCTCGTAAAGCATATGCGGAAACTAGGCCTAGGATTAAAGGGAGGTTTGCAAAACGCACTGATGCAGTTGATTCCATTTCTGGATACGGCATCGTTCCCACTTGTTGA
Protein sequence

>Msa0520760

MASKLCDSCKSATATLYCRPDSAFLCGACDSKVHAANKLASRHPRVTLCEVCEQAPAHVTCKADAASLCITCDRDIHTANPLAARHERVPVTPFFESNTSHSVKSLQSNNNNYDAVKDEAEVASWLISDPKADLNSSPYLFSDSEAIPFMDLDYGVIEHKNDGVVPVHGNFDPFVSAYKNNNVHLHTELETPSQSQISQSVSSSSMEVGVVPDGNTVSEISNCGYGTVAADREARVMRYREKRKNRRFEKTIRYASRKAYAETRPRIKGRFAKRTDAVDSISGYGIVPTC*