AlfalfaGEDB | Msa0434850

M. sativa cultivar zhongmu-4 / Msa0434850

KEGG: -
GO: GO:0001558 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0030308 GO:0030544 GO:0031072 GO:0031090 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031965 GO:0031967 GO:0031975 GO:0034641 GO:0034645 GO:0040008 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045893 GO:0045926 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0061649 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0140030 GO:0140035 GO:0140036 GO:1901360 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001141
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0434850	XP_013462122.1	94.505	91	5	0	1	91	1	91	1.43e-58	184

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0434850	sp\|F4JVB8\|RADL1_ARATH	59.770	87	33	1	7	91	14	100	1.40e-31	109

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0434850	A0A072V2R8	94.505	91	5	0	1	91	1	91	6.85e-59	184

TFs/TRs:

Gene ID	Type	Classification
Msa0434850	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0384120	Msa0434850	0.843490	1.357049e-58	-8.615850e-47
Msa0434850	Msa0522840	0.898835	3.592723e-77	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0434850	MtrunA17_Chr3g0141601	94.505	91	5	0	1	91	1	91	1.32e-62	184
Msa0434850	MtrunA17_Chr1g0155181	75.862	87	17	2	1	84	1	86	2.28e-43	135
Msa0434850	MtrunA17_Chr3g0144631	72.059	68	19	0	5	72	14	81	2.20e-33	110
Msa0434850	MtrunA17_Chr3g0081631	57.609	92	34	2	1	91	4	91	1.79e-32	108
Msa0434850	MtrunA17_Chr7g0255841	60.241	83	29	1	9	91	14	92	2.65e-29	100
Msa0434850	MtrunA17_Chr6g0449111	57.143	70	30	0	2	71	5	74	3.20e-26	92.0
Msa0434850	MtrunA17_Chr8g0373111	55.714	70	30	1	4	73	2	70	2.12e-25	90.1
Msa0434850	MtrunA17_Chr8g0373121	55.714	70	30	1	4	73	2	70	2.12e-25	90.1
Msa0434850	MtrunA17_Chr8g0373131	55.714	70	30	1	4	73	2	70	2.63e-25	89.7
Msa0434850	MtrunA17_Chr5g0404101	61.194	67	26	0	3	69	15	81	1.78e-24	88.2
Msa0434850	MtrunA17_Chr3g0141641	45.556	90	44	1	7	91	3	92	2.62e-24	87.8
Msa0434850	MtrunA17_Chr8g0373101	58.571	70	28	1	4	73	2	70	6.00e-24	86.3
Msa0434850	MtrunA17_Chr5g0404301	61.905	63	23	1	5	67	2	63	1.14e-22	82.8
Msa0434850	MtrunA17_Chr8g0364741	53.623	69	32	0	1	69	4	72	2.51e-21	81.3
Msa0434850	MtrunA17_Chr5g0429521	45.652	92	39	1	4	84	26	117	4.68e-21	84.3
Msa0434850	MtrunA17_Chr5g0436501	55.385	65	29	0	5	69	25	89	5.19e-21	83.6
Msa0434850	MtrunA17_Chr1g0162081	56.522	69	30	0	4	72	9	77	5.33e-21	84.0
Msa0434850	MtrunA17_Chr8g0365011	57.576	66	28	0	4	69	5	70	6.11e-21	83.6
Msa0434850	MtrunA17_Chr7g0241761	50.000	78	36	1	5	82	33	107	2.62e-20	82.4
Msa0434850	MtrunA17_Chr3g0104071	53.731	67	31	0	4	70	40	106	3.37e-19	79.7
Msa0434850	MtrunA17_Chr4g0062741	46.667	75	37	2	2	73	3	77	1.50e-17	74.7
Msa0434850	MtrunA17_Chr1g0210471	46.269	67	36	0	5	71	22	88	4.25e-17	72.8
Msa0434850	MtrunA17_Chr1g0194071	49.153	59	29	1	5	62	9	67	3.07e-14	62.4
Msa0434850	MtrunA17_Chr7g0270711	49.206	63	31	1	3	64	7	69	5.72e-14	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0434850	AT4G39250.1	59.770	87	33	1	7	91	14	100	1.42e-32	109
Msa0434850	AT2G21650.1	57.955	88	34	1	7	91	14	101	2.28e-31	106
Msa0434850	AT1G75250.1	72.059	68	19	0	2	69	7	74	5.69e-31	105
Msa0434850	AT1G75250.2	62.637	91	32	2	2	90	7	97	1.02e-30	104
Msa0434850	AT1G19510.1	68.182	66	20	1	10	74	15	80	1.16e-25	91.7
Msa0434850	AT2G18328.1	64.516	62	22	0	10	71	14	75	4.46e-25	89.7
Msa0434850	AT2G38090.1	52.174	69	33	0	4	72	26	94	5.50e-21	84.3
Msa0434850	AT5G58900.1	54.545	66	30	0	4	69	31	96	1.57e-20	83.2
Msa0434850	AT5G05790.2	47.436	78	39	1	4	79	28	105	1.55e-19	80.1
Msa0434850	AT5G05790.1	47.436	78	39	1	4	79	28	105	1.55e-19	80.1
Msa0434850	AT4G36570.1	72.727	44	12	0	7	50	12	55	2.84e-18	72.0
Msa0434850	AT3G11280.2	46.053	76	38	1	7	79	33	108	1.87e-17	74.7
Msa0434850	AT3G11280.1	46.053	76	38	1	7	79	33	108	1.87e-17	74.7
Msa0434850	AT5G08520.1	44.737	76	42	0	1	76	6	81	9.86e-16	70.5
Msa0434850	AT5G01200.1	34.951	103	49	2	5	89	28	130	1.43e-15	69.7
Msa0434850	AT1G49010.1	41.250	80	42	2	5	81	6	83	5.58e-14	65.5
Msa0434850	AT5G04760.1	40.299	67	39	1	3	69	2	67	6.73e-13	61.6
Msa0434850	AT5G23650.1	42.029	69	40	0	1	69	7	75	5.60e-12	60.1

Find 39 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCCCTCTCTATGAAGTATC+TGG	0.232989	3_2:+87880020	Msa0434850:three_prime_UTR
TAAAATTATTTAATTAAATC+TGG	0.281554	3_2:-87880228	None:intergenic
TTGGTTGCTACTACAATTAA+TGG	0.306877	3_2:+87879199	Msa0434850:exon
CCAACATTATTTGTGTAATT+AGG	0.311673	3_2:-87879416	None:intergenic
AGAACCTTGTTGAAGATATT+AGG	0.315510	3_2:+87879366	Msa0434850:CDS
ACATAATGGATCAAGATAAA+AGG	0.337165	3_2:+87879447	Msa0434850:CDS
AGGGACAGAGAGTAATAAAA+AGG	0.360193	3_2:-87880003	None:intergenic
TAATGGCTTCAAGTTCGAAT+TGG	0.366111	3_2:+87879216	Msa0434850:exon
CTTGCACAATCACCCTTTAG+TGG	0.367498	3_2:-87879951	None:intergenic
ATCATACGTAGTATTGTTGT+TGG	0.383323	3_2:+87879180	Msa0434850:five_prime_UTR
TGTTGGTTACAATTACATAA+TGG	0.412132	3_2:+87879433	Msa0434850:CDS
TCTCCACATGTATACTATAT+AGG	0.413310	3_2:-87880088	None:intergenic
CATTATTTGTGTAATTAGGC+AGG	0.435090	3_2:-87879412	None:intergenic
GGCTCTAAGTCTCCACTAAA+GGG	0.466211	3_2:+87879939	Msa0434850:exon
GCCACCTTCTGCCAACGATC+AGG	0.467098	3_2:-87879296	None:intergenic
TGAGAATGCGTTGGCAATGT+TGG	0.488680	3_2:+87879262	Msa0434850:CDS
TGTAATTAGGCAGGGGAACA+TGG	0.488710	3_2:-87879403	None:intergenic
CCTAATTACACAAATAATGT+TGG	0.495931	3_2:+87879416	Msa0434850:CDS
AGGCTCTAAGTCTCCACTAA+AGG	0.510302	3_2:+87879938	Msa0434850:CDS
TCCCTCTCTATGAAGTATCT+GGG	0.519538	3_2:+87880021	Msa0434850:three_prime_UTR
ATTATTTGTGTAATTAGGCA+GGG	0.547965	3_2:-87879411	None:intergenic
GCAGAAGGTGGCTAGAGCTG+TGG	0.552998	3_2:+87879307	Msa0434850:CDS
CAAAAGATTTGAGAATGCGT+TGG	0.553213	3_2:+87879253	Msa0434850:CDS
TCATGCATGATGCAGGATGA+AGG	0.557115	3_2:+87879918	Msa0434850:intron
CTACGTATGATGTTTAATGA+AGG	0.557260	3_2:-87879168	None:intergenic
CAGAAGGTGGCTAGAGCTGT+GGG	0.558074	3_2:+87879308	Msa0434850:CDS
CACTCCTGATCGTTGGCAGA+AGG	0.576549	3_2:+87879292	Msa0434850:CDS
GATATTAGGCAGATCGAGGA+AGG	0.589514	3_2:+87879380	Msa0434850:CDS
TTATTTGTGTAATTAGGCAG+GGG	0.601636	3_2:-87879410	None:intergenic
TCCTGATCGTTGGCAGAAGG+TGG	0.602919	3_2:+87879295	Msa0434850:CDS
GCCCAGATACTTCATAGAGA+GGG	0.610811	3_2:-87880022	None:intergenic
ACAAGGACACTCCTGATCGT+TGG	0.625458	3_2:+87879285	Msa0434850:CDS
TGCCCAGATACTTCATAGAG+AGG	0.629461	3_2:-87880023	None:intergenic
TGCGTTGGCAATGTTGGACA+AGG	0.631324	3_2:+87879268	Msa0434850:CDS
TTTCCTATATAGTATACATG+TGG	0.641933	3_2:+87880085	Msa0434850:three_prime_UTR
TGAAGATATTAGGCAGATCG+AGG	0.643314	3_2:+87879376	Msa0434850:CDS
AGGTGGCTAGAGCTGTGGGA+GGG	0.662019	3_2:+87879312	Msa0434850:CDS
AAGGTGGCTAGAGCTGTGGG+AGG	0.685328	3_2:+87879311	Msa0434850:CDS
TCTGCCTAATATCTTCAACA+AGG	0.692316	3_2:-87879370	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATTTATATGTTTATAAGT+AGG	+	chr3_2:87879710-87879729	Msa0434850:intron	10.0%
!!	GATCAAATAAATAATATAAT+TGG	-	chr3_2:87879873-87879892	None:intergenic	10.0%
!!	ATATTTATATGTTTATAAGT+AGG	+	chr3_2:87879710-87879729	Msa0434850:intron	10.0%
!!	GATCAAATAAATAATATAAT+TGG	-	chr3_2:87879873-87879892	None:intergenic	10.0%
!!	TATATTATTTATTTGATCTC+TGG	+	chr3_2:87879875-87879894	Msa0434850:intron	15.0%
!!	GATAAATGATAAAAACATAT+TGG	+	chr3_2:87880145-87880164	Msa0434850:three_prime_UTR	15.0%
!!!	TCAAATAAAATTTTAATCTC+AGG	+	chr3_2:87880189-87880208	Msa0434850:three_prime_UTR	15.0%
!!	TATATTATTTATTTGATCTC+TGG	+	chr3_2:87879875-87879894	Msa0434850:intron	15.0%
!!	GATAAATGATAAAAACATAT+TGG	+	chr3_2:87880145-87880164	Msa0434850:three_prime_UTR	15.0%
!!!	TCAAATAAAATTTTAATCTC+AGG	+	chr3_2:87880189-87880208	Msa0434850:three_prime_UTR	15.0%
!!!	TGATTTGTTGTAGATTAATA+TGG	+	chr3_2:87879540-87879559	Msa0434850:intron	20.0%
!!!	TTTATTCAAGTTTTTTGGTT+TGG	-	chr3_2:87879624-87879643	None:intergenic	20.0%
!!!	TTGGTTTTATTCAAGTTTTT+TGG	-	chr3_2:87879629-87879648	None:intergenic	20.0%
!!!	ATTGCTGTTTTAATTGTTTT+TGG	-	chr3_2:87879648-87879667	None:intergenic	20.0%
!!	GATATTCTTAAAGTTAAGAT+TGG	-	chr3_2:87879780-87879799	None:intergenic	20.0%
!!!	ATTTTATTTGACAGAACTAA+CGG	-	chr3_2:87880180-87880199	None:intergenic	20.0%
!!!	TGATTTGTTGTAGATTAATA+TGG	+	chr3_2:87879540-87879559	Msa0434850:intron	20.0%
!!!	TTTATTCAAGTTTTTTGGTT+TGG	-	chr3_2:87879624-87879643	None:intergenic	20.0%
!!!	TTGGTTTTATTCAAGTTTTT+TGG	-	chr3_2:87879629-87879648	None:intergenic	20.0%
!!!	ATTGCTGTTTTAATTGTTTT+TGG	-	chr3_2:87879648-87879667	None:intergenic	20.0%
!!	GATATTCTTAAAGTTAAGAT+TGG	-	chr3_2:87879780-87879799	None:intergenic	20.0%
!!!	ATTTTATTTGACAGAACTAA+CGG	-	chr3_2:87880180-87880199	None:intergenic	20.0%
!	ATTATTTGTGTAATTAGGCA+GGG	-	chr3_2:87879414-87879433	None:intergenic	25.0%
!	CCAACATTATTTGTGTAATT+AGG	-	chr3_2:87879419-87879438	None:intergenic	25.0%
!	CCTAATTACACAAATAATGT+TGG	+	chr3_2:87879416-87879435	Msa0434850:CDS	25.0%
!!	TGTTGGTTACAATTACATAA+TGG	+	chr3_2:87879433-87879452	Msa0434850:CDS	25.0%
!!!	ACATAATGGATCAAGATAAA+AGG	+	chr3_2:87879447-87879466	Msa0434850:CDS	25.0%
!!	TTCATGTGTAACAAACATTT+TGG	-	chr3_2:87879806-87879825	None:intergenic	25.0%
!	TGCATGTATGAATAATAATC+AGG	-	chr3_2:87879832-87879851	None:intergenic	25.0%
!	TGATTATTATTCATACATGC+AGG	+	chr3_2:87879831-87879850	Msa0434850:intron	25.0%
!	TTTCCTATATAGTATACATG+TGG	+	chr3_2:87880085-87880104	Msa0434850:three_prime_UTR	25.0%
!	ATTATTTGTGTAATTAGGCA+GGG	-	chr3_2:87879414-87879433	None:intergenic	25.0%
!	CCAACATTATTTGTGTAATT+AGG	-	chr3_2:87879419-87879438	None:intergenic	25.0%
!	CCTAATTACACAAATAATGT+TGG	+	chr3_2:87879416-87879435	Msa0434850:CDS	25.0%
!!	TGTTGGTTACAATTACATAA+TGG	+	chr3_2:87879433-87879452	Msa0434850:CDS	25.0%
!!!	ACATAATGGATCAAGATAAA+AGG	+	chr3_2:87879447-87879466	Msa0434850:CDS	25.0%
!!	TTCATGTGTAACAAACATTT+TGG	-	chr3_2:87879806-87879825	None:intergenic	25.0%
!	TGCATGTATGAATAATAATC+AGG	-	chr3_2:87879832-87879851	None:intergenic	25.0%
!	TGATTATTATTCATACATGC+AGG	+	chr3_2:87879831-87879850	Msa0434850:intron	25.0%
!	TTTCCTATATAGTATACATG+TGG	+	chr3_2:87880085-87880104	Msa0434850:three_prime_UTR	25.0%
	CTACGTATGATGTTTAATGA+AGG	-	chr3_2:87879171-87879190	None:intergenic	30.0%
	ATCATACGTAGTATTGTTGT+TGG	+	chr3_2:87879180-87879199	Msa0434850:five_prime_UTR	30.0%
	TTGGTTGCTACTACAATTAA+TGG	+	chr3_2:87879199-87879218	Msa0434850:exon	30.0%
!	AAATCTTTTGTTCTGCTTTG+CGG	-	chr3_2:87879243-87879262	None:intergenic	30.0%
	AGAACCTTGTTGAAGATATT+AGG	+	chr3_2:87879366-87879385	Msa0434850:CDS	30.0%
	TTATTTGTGTAATTAGGCAG+GGG	-	chr3_2:87879413-87879432	None:intergenic	30.0%
	CATTATTTGTGTAATTAGGC+AGG	-	chr3_2:87879415-87879434	None:intergenic	30.0%
!!	CATTGTGTTTGATTGGTTTA+AGG	-	chr3_2:87879566-87879585	None:intergenic	30.0%
	CTAGTTTCATTGTGTTTGAT+TGG	-	chr3_2:87879573-87879592	None:intergenic	30.0%
!	ACTTTACATAACTAGCTTGA+AGG	-	chr3_2:87879748-87879767	None:intergenic	30.0%
	TCTCCACATGTATACTATAT+AGG	-	chr3_2:87880091-87880110	None:intergenic	30.0%
	CTACGTATGATGTTTAATGA+AGG	-	chr3_2:87879171-87879190	None:intergenic	30.0%
	ATCATACGTAGTATTGTTGT+TGG	+	chr3_2:87879180-87879199	Msa0434850:five_prime_UTR	30.0%
	TTGGTTGCTACTACAATTAA+TGG	+	chr3_2:87879199-87879218	Msa0434850:exon	30.0%
!	AAATCTTTTGTTCTGCTTTG+CGG	-	chr3_2:87879243-87879262	None:intergenic	30.0%
	AGAACCTTGTTGAAGATATT+AGG	+	chr3_2:87879366-87879385	Msa0434850:CDS	30.0%
	TTATTTGTGTAATTAGGCAG+GGG	-	chr3_2:87879413-87879432	None:intergenic	30.0%
	CATTATTTGTGTAATTAGGC+AGG	-	chr3_2:87879415-87879434	None:intergenic	30.0%
!!	CATTGTGTTTGATTGGTTTA+AGG	-	chr3_2:87879566-87879585	None:intergenic	30.0%
	CTAGTTTCATTGTGTTTGAT+TGG	-	chr3_2:87879573-87879592	None:intergenic	30.0%
!	ACTTTACATAACTAGCTTGA+AGG	-	chr3_2:87879748-87879767	None:intergenic	30.0%
	TCTCCACATGTATACTATAT+AGG	-	chr3_2:87880091-87880110	None:intergenic	30.0%
!	TAATGGCTTCAAGTTCGAAT+TGG	+	chr3_2:87879216-87879235	Msa0434850:exon	35.0%
	CAAAAGATTTGAGAATGCGT+TGG	+	chr3_2:87879253-87879272	Msa0434850:CDS	35.0%
	TCTGCCTAATATCTTCAACA+AGG	-	chr3_2:87879373-87879392	None:intergenic	35.0%
	GTATTTATCATGCATGATGC+AGG	+	chr3_2:87879911-87879930	Msa0434850:intron	35.0%
	AGGGACAGAGAGTAATAAAA+AGG	-	chr3_2:87880006-87880025	None:intergenic	35.0%
!	TAATGGCTTCAAGTTCGAAT+TGG	+	chr3_2:87879216-87879235	Msa0434850:exon	35.0%
	CAAAAGATTTGAGAATGCGT+TGG	+	chr3_2:87879253-87879272	Msa0434850:CDS	35.0%
	TCTGCCTAATATCTTCAACA+AGG	-	chr3_2:87879373-87879392	None:intergenic	35.0%
	GTATTTATCATGCATGATGC+AGG	+	chr3_2:87879911-87879930	Msa0434850:intron	35.0%
	AGGGACAGAGAGTAATAAAA+AGG	-	chr3_2:87880006-87880025	None:intergenic	35.0%
	TGAAGATATTAGGCAGATCG+AGG	+	chr3_2:87879376-87879395	Msa0434850:CDS	40.0%
!	TCCCTCTCTATGAAGTATCT+GGG	+	chr3_2:87880021-87880040	Msa0434850:three_prime_UTR	40.0%
	TGAAGATATTAGGCAGATCG+AGG	+	chr3_2:87879376-87879395	Msa0434850:CDS	40.0%
!	TCCCTCTCTATGAAGTATCT+GGG	+	chr3_2:87880021-87880040	Msa0434850:three_prime_UTR	40.0%
!	TGAGAATGCGTTGGCAATGT+TGG	+	chr3_2:87879262-87879281	Msa0434850:CDS	45.0%
	GATATTAGGCAGATCGAGGA+AGG	+	chr3_2:87879380-87879399	Msa0434850:CDS	45.0%
	TGTAATTAGGCAGGGGAACA+TGG	-	chr3_2:87879406-87879425	None:intergenic	45.0%
	TCATGCATGATGCAGGATGA+AGG	+	chr3_2:87879918-87879937	Msa0434850:intron	45.0%
!!	AGGCTCTAAGTCTCCACTAA+AGG	+	chr3_2:87879938-87879957	Msa0434850:CDS	45.0%
!!	GGCTCTAAGTCTCCACTAAA+GGG	+	chr3_2:87879939-87879958	Msa0434850:exon	45.0%
	CTTGCACAATCACCCTTTAG+TGG	-	chr3_2:87879954-87879973	None:intergenic	45.0%
!	GTCCCTCTCTATGAAGTATC+TGG	+	chr3_2:87880020-87880039	Msa0434850:three_prime_UTR	45.0%
	GCCCAGATACTTCATAGAGA+GGG	-	chr3_2:87880025-87880044	None:intergenic	45.0%
	TGCCCAGATACTTCATAGAG+AGG	-	chr3_2:87880026-87880045	None:intergenic	45.0%
!	TGAGAATGCGTTGGCAATGT+TGG	+	chr3_2:87879262-87879281	Msa0434850:CDS	45.0%
	GATATTAGGCAGATCGAGGA+AGG	+	chr3_2:87879380-87879399	Msa0434850:CDS	45.0%
	TGTAATTAGGCAGGGGAACA+TGG	-	chr3_2:87879406-87879425	None:intergenic	45.0%
	TCATGCATGATGCAGGATGA+AGG	+	chr3_2:87879918-87879937	Msa0434850:intron	45.0%
!!	AGGCTCTAAGTCTCCACTAA+AGG	+	chr3_2:87879938-87879957	Msa0434850:CDS	45.0%
!!	GGCTCTAAGTCTCCACTAAA+GGG	+	chr3_2:87879939-87879958	Msa0434850:exon	45.0%
	CTTGCACAATCACCCTTTAG+TGG	-	chr3_2:87879954-87879973	None:intergenic	45.0%
!	GTCCCTCTCTATGAAGTATC+TGG	+	chr3_2:87880020-87880039	Msa0434850:three_prime_UTR	45.0%
	GCCCAGATACTTCATAGAGA+GGG	-	chr3_2:87880025-87880044	None:intergenic	45.0%
	TGCCCAGATACTTCATAGAG+AGG	-	chr3_2:87880026-87880045	None:intergenic	45.0%
!!	TAATATATATAAATTGAAAT+TGG	-	chr3_2:87879497-87879516	None:intergenic	5.0%
!!	TAAAATTATTTAATTAAATC+TGG	-	chr3_2:87880231-87880250	None:intergenic	5.0%
!!	TAATATATATAAATTGAAAT+TGG	-	chr3_2:87879497-87879516	None:intergenic	5.0%
!!	TAAAATTATTTAATTAAATC+TGG	-	chr3_2:87880231-87880250	None:intergenic	5.0%
!	TGCGTTGGCAATGTTGGACA+AGG	+	chr3_2:87879268-87879287	Msa0434850:CDS	50.0%
	ACAAGGACACTCCTGATCGT+TGG	+	chr3_2:87879285-87879304	Msa0434850:CDS	50.0%
!	TGCGTTGGCAATGTTGGACA+AGG	+	chr3_2:87879268-87879287	Msa0434850:CDS	50.0%
	ACAAGGACACTCCTGATCGT+TGG	+	chr3_2:87879285-87879304	Msa0434850:CDS	50.0%
	CACTCCTGATCGTTGGCAGA+AGG	+	chr3_2:87879292-87879311	Msa0434850:CDS	55.0%
	TCCTGATCGTTGGCAGAAGG+TGG	+	chr3_2:87879295-87879314	Msa0434850:CDS	55.0%
	CAGAAGGTGGCTAGAGCTGT+GGG	+	chr3_2:87879308-87879327	Msa0434850:CDS	55.0%
	CACTCCTGATCGTTGGCAGA+AGG	+	chr3_2:87879292-87879311	Msa0434850:CDS	55.0%
	TCCTGATCGTTGGCAGAAGG+TGG	+	chr3_2:87879295-87879314	Msa0434850:CDS	55.0%
	CAGAAGGTGGCTAGAGCTGT+GGG	+	chr3_2:87879308-87879327	Msa0434850:CDS	55.0%
	GCCACCTTCTGCCAACGATC+AGG	-	chr3_2:87879299-87879318	None:intergenic	60.0%
	GCAGAAGGTGGCTAGAGCTG+TGG	+	chr3_2:87879307-87879326	Msa0434850:CDS	60.0%
	AAGGTGGCTAGAGCTGTGGG+AGG	+	chr3_2:87879311-87879330	Msa0434850:CDS	60.0%
	AGGTGGCTAGAGCTGTGGGA+GGG	+	chr3_2:87879312-87879331	Msa0434850:CDS	60.0%
	GCCACCTTCTGCCAACGATC+AGG	-	chr3_2:87879299-87879318	None:intergenic	60.0%
	GCAGAAGGTGGCTAGAGCTG+TGG	+	chr3_2:87879307-87879326	Msa0434850:CDS	60.0%
	AAGGTGGCTAGAGCTGTGGG+AGG	+	chr3_2:87879311-87879330	Msa0434850:CDS	60.0%
	AGGTGGCTAGAGCTGTGGGA+GGG	+	chr3_2:87879312-87879331	Msa0434850:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	87879154	87880265	87879154	ID=Msa0434850;Name=Msa0434850
chr3_2	mRNA	87879154	87880265	87879154	ID=Msa0434850-mRNA-1;Parent=Msa0434850;Name=Msa0434850-mRNA-1;_AED=0.01;_eAED=0.01;_QI=64\|1\|1\|1\|1\|1\|2\|308\|91
chr3_2	exon	87879154	87879468	87879154	ID=Msa0434850-mRNA-1:exon:25642;Parent=Msa0434850-mRNA-1
chr3_2	exon	87879933	87880265	87879933	ID=Msa0434850-mRNA-1:exon:25643;Parent=Msa0434850-mRNA-1
chr3_2	five_prime_UTR	87879154	87879217	87879154	ID=Msa0434850-mRNA-1:five_prime_utr;Parent=Msa0434850-mRNA-1
chr3_2	CDS	87879218	87879468	87879218	ID=Msa0434850-mRNA-1:cds;Parent=Msa0434850-mRNA-1
chr3_2	CDS	87879933	87879957	87879933	ID=Msa0434850-mRNA-1:cds;Parent=Msa0434850-mRNA-1
chr3_2	three_prime_UTR	87879958	87880265	87879958	ID=Msa0434850-mRNA-1:three_prime_utr;Parent=Msa0434850-mRNA-1

Gene Sequence	>Msa0434850 ATGGCTTCAAGTTCGAATTGGACCGCAAAGCAGAACAAAAGATTTGAGAATGCGTTGGCAATGTTGGACAAGGACACTCCTGATCGTTGGCAGAAGGTGGCTAGAGCTGTGGGAGGGAAGACTGTTGAAGAAGTGAAAAGACATTATGAGAACCTTGTTGAAGATATTAGGCAGATCGAGGAAGGCCATGTTCCCCTGCCTAATTACACAAATAATGTTGGTTACAATTACATAATGGATCAAGATAAAAGGATGAAGGCTCTAAGTCTCCACTAA
Protein sequence	>Msa0434850 MASSSNWTAKQNKRFENALAMLDKDTPDRWQKVARAVGGKTVEEVKRHYENLVEDIRQIEEGHVPLPNYTNNVGYNYIMDQDKRMKALSLH*