AlfalfaGEDB | Msa0522840

M. sativa cultivar zhongmu-4 / Msa0522840

KEGG: -
GO: GO:0001558 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0030308 GO:0030544 GO:0031072 GO:0031090 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031965 GO:0031967 GO:0031975 GO:0034641 GO:0034645 GO:0040008 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045893 GO:0045926 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0061649 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0140030 GO:0140035 GO:0140036 GO:1901360 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001141
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522840	XP_013462122.1	94.505	91	5	0	1	91	1	91	1.43e-58	184

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522840	sp\|F4JVB8\|RADL1_ARATH	59.770	87	33	1	7	91	14	100	1.40e-31	109

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522840	A0A072V2R8	94.505	91	5	0	1	91	1	91	6.85e-59	184

TFs/TRs:

Gene ID	Type	Classification
Msa0522840	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0384120	Msa0522840	0.855917	4.572827e-62	-8.615850e-47
Msa0434850	Msa0522840	0.898835	3.592723e-77	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522840	MtrunA17_Chr3g0141601	94.505	91	5	0	1	91	1	91	1.32e-62	184
Msa0522840	MtrunA17_Chr1g0155181	75.862	87	17	2	1	84	1	86	2.28e-43	135
Msa0522840	MtrunA17_Chr3g0144631	72.059	68	19	0	5	72	14	81	2.20e-33	110
Msa0522840	MtrunA17_Chr3g0081631	57.609	92	34	2	1	91	4	91	1.79e-32	108
Msa0522840	MtrunA17_Chr7g0255841	60.241	83	29	1	9	91	14	92	2.65e-29	100
Msa0522840	MtrunA17_Chr6g0449111	57.143	70	30	0	2	71	5	74	3.20e-26	92.0
Msa0522840	MtrunA17_Chr8g0373111	55.714	70	30	1	4	73	2	70	2.12e-25	90.1
Msa0522840	MtrunA17_Chr8g0373121	55.714	70	30	1	4	73	2	70	2.12e-25	90.1
Msa0522840	MtrunA17_Chr8g0373131	55.714	70	30	1	4	73	2	70	2.63e-25	89.7
Msa0522840	MtrunA17_Chr5g0404101	61.194	67	26	0	3	69	15	81	1.78e-24	88.2
Msa0522840	MtrunA17_Chr3g0141641	45.556	90	44	1	7	91	3	92	2.62e-24	87.8
Msa0522840	MtrunA17_Chr8g0373101	58.571	70	28	1	4	73	2	70	6.00e-24	86.3
Msa0522840	MtrunA17_Chr5g0404301	61.905	63	23	1	5	67	2	63	1.14e-22	82.8
Msa0522840	MtrunA17_Chr8g0364741	53.623	69	32	0	1	69	4	72	2.51e-21	81.3
Msa0522840	MtrunA17_Chr5g0429521	45.652	92	39	1	4	84	26	117	4.68e-21	84.3
Msa0522840	MtrunA17_Chr5g0436501	55.385	65	29	0	5	69	25	89	5.19e-21	83.6
Msa0522840	MtrunA17_Chr1g0162081	56.522	69	30	0	4	72	9	77	5.33e-21	84.0
Msa0522840	MtrunA17_Chr8g0365011	57.576	66	28	0	4	69	5	70	6.11e-21	83.6
Msa0522840	MtrunA17_Chr7g0241761	50.000	78	36	1	5	82	33	107	2.62e-20	82.4
Msa0522840	MtrunA17_Chr3g0104071	53.731	67	31	0	4	70	40	106	3.37e-19	79.7
Msa0522840	MtrunA17_Chr4g0062741	46.667	75	37	2	2	73	3	77	1.50e-17	74.7
Msa0522840	MtrunA17_Chr1g0210471	46.269	67	36	0	5	71	22	88	4.25e-17	72.8
Msa0522840	MtrunA17_Chr1g0194071	49.153	59	29	1	5	62	9	67	3.07e-14	62.4
Msa0522840	MtrunA17_Chr7g0270711	49.206	63	31	1	3	64	7	69	5.72e-14	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0522840	AT4G39250.1	59.770	87	33	1	7	91	14	100	1.42e-32	109
Msa0522840	AT2G21650.1	57.955	88	34	1	7	91	14	101	2.28e-31	106
Msa0522840	AT1G75250.1	72.059	68	19	0	2	69	7	74	5.69e-31	105
Msa0522840	AT1G75250.2	62.637	91	32	2	2	90	7	97	1.02e-30	104
Msa0522840	AT1G19510.1	68.182	66	20	1	10	74	15	80	1.16e-25	91.7
Msa0522840	AT2G18328.1	64.516	62	22	0	10	71	14	75	4.46e-25	89.7
Msa0522840	AT2G38090.1	52.174	69	33	0	4	72	26	94	5.50e-21	84.3
Msa0522840	AT5G58900.1	54.545	66	30	0	4	69	31	96	1.57e-20	83.2
Msa0522840	AT5G05790.2	47.436	78	39	1	4	79	28	105	1.55e-19	80.1
Msa0522840	AT5G05790.1	47.436	78	39	1	4	79	28	105	1.55e-19	80.1
Msa0522840	AT4G36570.1	72.727	44	12	0	7	50	12	55	2.84e-18	72.0
Msa0522840	AT3G11280.2	46.053	76	38	1	7	79	33	108	1.87e-17	74.7
Msa0522840	AT3G11280.1	46.053	76	38	1	7	79	33	108	1.87e-17	74.7
Msa0522840	AT5G08520.1	44.737	76	42	0	1	76	6	81	9.86e-16	70.5
Msa0522840	AT5G01200.1	34.951	103	49	2	5	89	28	130	1.43e-15	69.7
Msa0522840	AT1G49010.1	41.250	80	42	2	5	81	6	83	5.58e-14	65.5
Msa0522840	AT5G04760.1	40.299	67	39	1	3	69	2	67	6.73e-13	61.6
Msa0522840	AT5G23650.1	42.029	69	40	0	1	69	7	75	5.60e-12	60.1

Find 39 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCCCTCTCTATGAAGTATC+TGG	0.232989	3_4:+76164408	Msa0522840:three_prime_UTR
TAAAATTATTTAATTAAATC+TGG	0.281554	3_4:-76164616	None:intergenic
TTGGTTGCTACTACAATTAA+TGG	0.306877	3_4:+76163618	Msa0522840:exon
CCAACATTATTTGTGTAATT+AGG	0.311673	3_4:-76163835	None:intergenic
AGAACCTTGTTGAAGATATT+AGG	0.315510	3_4:+76163785	Msa0522840:CDS
ACATAATGGATCAAGATAAA+AGG	0.337165	3_4:+76163866	Msa0522840:CDS
AGGGACAGAGAGTAATAAAA+AGG	0.360193	3_4:-76164391	None:intergenic
TAATGGCTTCAAGTTCGAAT+TGG	0.366111	3_4:+76163635	Msa0522840:exon
CTTGCACAATCACCCTTTAG+TGG	0.367498	3_4:-76164339	None:intergenic
ATCATACGTAGTATTGTTGT+TGG	0.383323	3_4:+76163599	Msa0522840:five_prime_UTR
TGTTGGTTACAATTACATAA+TGG	0.412132	3_4:+76163852	Msa0522840:CDS
TCTCCACATGTATACTATAT+AGG	0.413310	3_4:-76164476	None:intergenic
CATTATTTGTGTAATTAGGC+AGG	0.435090	3_4:-76163831	None:intergenic
GGCTCTAAGTCTCCACTAAA+GGG	0.466211	3_4:+76164327	Msa0522840:exon
GCCACCTTCTGCCAACGATC+AGG	0.467098	3_4:-76163715	None:intergenic
TGAGAATGCGTTGGCAATGT+TGG	0.488680	3_4:+76163681	Msa0522840:CDS
TGTAATTAGGCAGGGGAACA+TGG	0.488710	3_4:-76163822	None:intergenic
CCTAATTACACAAATAATGT+TGG	0.495931	3_4:+76163835	Msa0522840:CDS
AGGCTCTAAGTCTCCACTAA+AGG	0.510302	3_4:+76164326	Msa0522840:CDS
TCCCTCTCTATGAAGTATCT+GGG	0.519538	3_4:+76164409	Msa0522840:three_prime_UTR
ATTATTTGTGTAATTAGGCA+GGG	0.547965	3_4:-76163830	None:intergenic
GCAGAAGGTGGCTAGAGCTG+TGG	0.552998	3_4:+76163726	Msa0522840:CDS
CAAAAGATTTGAGAATGCGT+TGG	0.553213	3_4:+76163672	Msa0522840:CDS
TCATGCATGATGCAGGATGA+AGG	0.557115	3_4:+76164306	Msa0522840:intron
CTACGTATGATGTTTAATGA+AGG	0.557260	3_4:-76163587	None:intergenic
CAGAAGGTGGCTAGAGCTGT+GGG	0.558074	3_4:+76163727	Msa0522840:CDS
CACTCCTGATCGTTGGCAGA+AGG	0.576549	3_4:+76163711	Msa0522840:CDS
GATATTAGGCAGATCGAGGA+AGG	0.589514	3_4:+76163799	Msa0522840:CDS
TTATTTGTGTAATTAGGCAG+GGG	0.601636	3_4:-76163829	None:intergenic
TCCTGATCGTTGGCAGAAGG+TGG	0.602919	3_4:+76163714	Msa0522840:CDS
GCCCAGATACTTCATAGAGA+GGG	0.610811	3_4:-76164410	None:intergenic
ACAAGGACACTCCTGATCGT+TGG	0.625458	3_4:+76163704	Msa0522840:CDS
TGCCCAGATACTTCATAGAG+AGG	0.629461	3_4:-76164411	None:intergenic
TGCGTTGGCAATGTTGGACA+AGG	0.631324	3_4:+76163687	Msa0522840:CDS
TTTCCTATATAGTATACATG+TGG	0.641933	3_4:+76164473	Msa0522840:three_prime_UTR
TGAAGATATTAGGCAGATCG+AGG	0.643314	3_4:+76163795	Msa0522840:CDS
AGGTGGCTAGAGCTGTGGGA+GGG	0.662019	3_4:+76163731	Msa0522840:CDS
AAGGTGGCTAGAGCTGTGGG+AGG	0.685328	3_4:+76163730	Msa0522840:CDS
TCTGCCTAATATCTTCAACA+AGG	0.692316	3_4:-76163789	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAAATATATAAATTGAAAT+TGG	-	chr3_4:76163916-76163935	None:intergenic	10.0%
!!	ATATTTATATGTTTATAAGT+AGG	+	chr3_4:76164098-76164117	Msa0522840:intron	10.0%
!!	GATCAAATAAATAATATAAT+TGG	-	chr3_4:76164261-76164280	None:intergenic	10.0%
!!	ACAAATATATAAATTGAAAT+TGG	-	chr3_4:76163916-76163935	None:intergenic	10.0%
!!	ATATTTATATGTTTATAAGT+AGG	+	chr3_4:76164098-76164117	Msa0522840:intron	10.0%
!!	GATCAAATAAATAATATAAT+TGG	-	chr3_4:76164261-76164280	None:intergenic	10.0%
!!!	ATATTTGTTGTAGATTAATA+TGG	+	chr3_4:76163928-76163947	Msa0522840:intron	15.0%
!!	TATATTATTTATTTGATCTC+TGG	+	chr3_4:76164263-76164282	Msa0522840:intron	15.0%
!!	GATAAATGATAAAAACATAT+TGG	+	chr3_4:76164533-76164552	Msa0522840:three_prime_UTR	15.0%
!!!	TCAAATAAAATTTTAATCTC+AGG	+	chr3_4:76164577-76164596	Msa0522840:three_prime_UTR	15.0%
!!!	ATATTTGTTGTAGATTAATA+TGG	+	chr3_4:76163928-76163947	Msa0522840:intron	15.0%
!!	TATATTATTTATTTGATCTC+TGG	+	chr3_4:76164263-76164282	Msa0522840:intron	15.0%
!!	GATAAATGATAAAAACATAT+TGG	+	chr3_4:76164533-76164552	Msa0522840:three_prime_UTR	15.0%
!!!	TCAAATAAAATTTTAATCTC+AGG	+	chr3_4:76164577-76164596	Msa0522840:three_prime_UTR	15.0%
!!!	TTTATTCAAGTTTTTTGGTT+TGG	-	chr3_4:76164012-76164031	None:intergenic	20.0%
!!!	TTGGTTTTATTCAAGTTTTT+TGG	-	chr3_4:76164017-76164036	None:intergenic	20.0%
!!!	ATTGCTGTTTTAATTGTTTT+TGG	-	chr3_4:76164036-76164055	None:intergenic	20.0%
!!	GATATTCTTAAAGTTAAGAT+TGG	-	chr3_4:76164168-76164187	None:intergenic	20.0%
!!!	ATTTTATTTGACAGAACTAA+CGG	-	chr3_4:76164568-76164587	None:intergenic	20.0%
!!!	TTTATTCAAGTTTTTTGGTT+TGG	-	chr3_4:76164012-76164031	None:intergenic	20.0%
!!!	TTGGTTTTATTCAAGTTTTT+TGG	-	chr3_4:76164017-76164036	None:intergenic	20.0%
!!!	ATTGCTGTTTTAATTGTTTT+TGG	-	chr3_4:76164036-76164055	None:intergenic	20.0%
!!	GATATTCTTAAAGTTAAGAT+TGG	-	chr3_4:76164168-76164187	None:intergenic	20.0%
!!!	ATTTTATTTGACAGAACTAA+CGG	-	chr3_4:76164568-76164587	None:intergenic	20.0%
!	ATTATTTGTGTAATTAGGCA+GGG	-	chr3_4:76163833-76163852	None:intergenic	25.0%
!	CCAACATTATTTGTGTAATT+AGG	-	chr3_4:76163838-76163857	None:intergenic	25.0%
!	CCTAATTACACAAATAATGT+TGG	+	chr3_4:76163835-76163854	Msa0522840:CDS	25.0%
!!	TGTTGGTTACAATTACATAA+TGG	+	chr3_4:76163852-76163871	Msa0522840:CDS	25.0%
!!!	ACATAATGGATCAAGATAAA+AGG	+	chr3_4:76163866-76163885	Msa0522840:CDS	25.0%
!!	TTCATGTGTAACAAACATTT+TGG	-	chr3_4:76164194-76164213	None:intergenic	25.0%
!	TGCATGTATGAATAATAATC+AGG	-	chr3_4:76164220-76164239	None:intergenic	25.0%
!	TGATTATTATTCATACATGC+AGG	+	chr3_4:76164219-76164238	Msa0522840:intron	25.0%
!	TTTCCTATATAGTATACATG+TGG	+	chr3_4:76164473-76164492	Msa0522840:three_prime_UTR	25.0%
!	ATTATTTGTGTAATTAGGCA+GGG	-	chr3_4:76163833-76163852	None:intergenic	25.0%
!	CCAACATTATTTGTGTAATT+AGG	-	chr3_4:76163838-76163857	None:intergenic	25.0%
!	CCTAATTACACAAATAATGT+TGG	+	chr3_4:76163835-76163854	Msa0522840:CDS	25.0%
!!	TGTTGGTTACAATTACATAA+TGG	+	chr3_4:76163852-76163871	Msa0522840:CDS	25.0%
!!!	ACATAATGGATCAAGATAAA+AGG	+	chr3_4:76163866-76163885	Msa0522840:CDS	25.0%
!!	TTCATGTGTAACAAACATTT+TGG	-	chr3_4:76164194-76164213	None:intergenic	25.0%
!	TGCATGTATGAATAATAATC+AGG	-	chr3_4:76164220-76164239	None:intergenic	25.0%
!	TGATTATTATTCATACATGC+AGG	+	chr3_4:76164219-76164238	Msa0522840:intron	25.0%
!	TTTCCTATATAGTATACATG+TGG	+	chr3_4:76164473-76164492	Msa0522840:three_prime_UTR	25.0%
	CTACGTATGATGTTTAATGA+AGG	-	chr3_4:76163590-76163609	None:intergenic	30.0%
	ATCATACGTAGTATTGTTGT+TGG	+	chr3_4:76163599-76163618	Msa0522840:five_prime_UTR	30.0%
	TTGGTTGCTACTACAATTAA+TGG	+	chr3_4:76163618-76163637	Msa0522840:exon	30.0%
!	AAATCTTTTGTTCTGCTTTG+CGG	-	chr3_4:76163662-76163681	None:intergenic	30.0%
	AGAACCTTGTTGAAGATATT+AGG	+	chr3_4:76163785-76163804	Msa0522840:CDS	30.0%
	TTATTTGTGTAATTAGGCAG+GGG	-	chr3_4:76163832-76163851	None:intergenic	30.0%
	CATTATTTGTGTAATTAGGC+AGG	-	chr3_4:76163834-76163853	None:intergenic	30.0%
!!	CATTGTGTTTGATTGGTTTA+AGG	-	chr3_4:76163954-76163973	None:intergenic	30.0%
	CTAGTTTCATTGTGTTTGAT+TGG	-	chr3_4:76163961-76163980	None:intergenic	30.0%
!	ACTTTACATAACTAGCTTGA+AGG	-	chr3_4:76164136-76164155	None:intergenic	30.0%
	TCTCCACATGTATACTATAT+AGG	-	chr3_4:76164479-76164498	None:intergenic	30.0%
	CTACGTATGATGTTTAATGA+AGG	-	chr3_4:76163590-76163609	None:intergenic	30.0%
	ATCATACGTAGTATTGTTGT+TGG	+	chr3_4:76163599-76163618	Msa0522840:five_prime_UTR	30.0%
	TTGGTTGCTACTACAATTAA+TGG	+	chr3_4:76163618-76163637	Msa0522840:exon	30.0%
!	AAATCTTTTGTTCTGCTTTG+CGG	-	chr3_4:76163662-76163681	None:intergenic	30.0%
	AGAACCTTGTTGAAGATATT+AGG	+	chr3_4:76163785-76163804	Msa0522840:CDS	30.0%
	TTATTTGTGTAATTAGGCAG+GGG	-	chr3_4:76163832-76163851	None:intergenic	30.0%
	CATTATTTGTGTAATTAGGC+AGG	-	chr3_4:76163834-76163853	None:intergenic	30.0%
!!	CATTGTGTTTGATTGGTTTA+AGG	-	chr3_4:76163954-76163973	None:intergenic	30.0%
	CTAGTTTCATTGTGTTTGAT+TGG	-	chr3_4:76163961-76163980	None:intergenic	30.0%
!	ACTTTACATAACTAGCTTGA+AGG	-	chr3_4:76164136-76164155	None:intergenic	30.0%
	TCTCCACATGTATACTATAT+AGG	-	chr3_4:76164479-76164498	None:intergenic	30.0%
!	TAATGGCTTCAAGTTCGAAT+TGG	+	chr3_4:76163635-76163654	Msa0522840:exon	35.0%
	CAAAAGATTTGAGAATGCGT+TGG	+	chr3_4:76163672-76163691	Msa0522840:CDS	35.0%
	TCTGCCTAATATCTTCAACA+AGG	-	chr3_4:76163792-76163811	None:intergenic	35.0%
	GTATTTATCATGCATGATGC+AGG	+	chr3_4:76164299-76164318	Msa0522840:intron	35.0%
	AGGGACAGAGAGTAATAAAA+AGG	-	chr3_4:76164394-76164413	None:intergenic	35.0%
!	TAATGGCTTCAAGTTCGAAT+TGG	+	chr3_4:76163635-76163654	Msa0522840:exon	35.0%
	CAAAAGATTTGAGAATGCGT+TGG	+	chr3_4:76163672-76163691	Msa0522840:CDS	35.0%
	TCTGCCTAATATCTTCAACA+AGG	-	chr3_4:76163792-76163811	None:intergenic	35.0%
	GTATTTATCATGCATGATGC+AGG	+	chr3_4:76164299-76164318	Msa0522840:intron	35.0%
	AGGGACAGAGAGTAATAAAA+AGG	-	chr3_4:76164394-76164413	None:intergenic	35.0%
	TGAAGATATTAGGCAGATCG+AGG	+	chr3_4:76163795-76163814	Msa0522840:CDS	40.0%
!	TCCCTCTCTATGAAGTATCT+GGG	+	chr3_4:76164409-76164428	Msa0522840:three_prime_UTR	40.0%
	TGAAGATATTAGGCAGATCG+AGG	+	chr3_4:76163795-76163814	Msa0522840:CDS	40.0%
!	TCCCTCTCTATGAAGTATCT+GGG	+	chr3_4:76164409-76164428	Msa0522840:three_prime_UTR	40.0%
!	TGAGAATGCGTTGGCAATGT+TGG	+	chr3_4:76163681-76163700	Msa0522840:CDS	45.0%
	GATATTAGGCAGATCGAGGA+AGG	+	chr3_4:76163799-76163818	Msa0522840:CDS	45.0%
	TGTAATTAGGCAGGGGAACA+TGG	-	chr3_4:76163825-76163844	None:intergenic	45.0%
	TCATGCATGATGCAGGATGA+AGG	+	chr3_4:76164306-76164325	Msa0522840:intron	45.0%
!!	AGGCTCTAAGTCTCCACTAA+AGG	+	chr3_4:76164326-76164345	Msa0522840:CDS	45.0%
!!	GGCTCTAAGTCTCCACTAAA+GGG	+	chr3_4:76164327-76164346	Msa0522840:exon	45.0%
	CTTGCACAATCACCCTTTAG+TGG	-	chr3_4:76164342-76164361	None:intergenic	45.0%
!	GTCCCTCTCTATGAAGTATC+TGG	+	chr3_4:76164408-76164427	Msa0522840:three_prime_UTR	45.0%
	GCCCAGATACTTCATAGAGA+GGG	-	chr3_4:76164413-76164432	None:intergenic	45.0%
	TGCCCAGATACTTCATAGAG+AGG	-	chr3_4:76164414-76164433	None:intergenic	45.0%
!	TGAGAATGCGTTGGCAATGT+TGG	+	chr3_4:76163681-76163700	Msa0522840:CDS	45.0%
	GATATTAGGCAGATCGAGGA+AGG	+	chr3_4:76163799-76163818	Msa0522840:CDS	45.0%
	TGTAATTAGGCAGGGGAACA+TGG	-	chr3_4:76163825-76163844	None:intergenic	45.0%
	TCATGCATGATGCAGGATGA+AGG	+	chr3_4:76164306-76164325	Msa0522840:intron	45.0%
!!	AGGCTCTAAGTCTCCACTAA+AGG	+	chr3_4:76164326-76164345	Msa0522840:CDS	45.0%
!!	GGCTCTAAGTCTCCACTAAA+GGG	+	chr3_4:76164327-76164346	Msa0522840:exon	45.0%
	CTTGCACAATCACCCTTTAG+TGG	-	chr3_4:76164342-76164361	None:intergenic	45.0%
!	GTCCCTCTCTATGAAGTATC+TGG	+	chr3_4:76164408-76164427	Msa0522840:three_prime_UTR	45.0%
	GCCCAGATACTTCATAGAGA+GGG	-	chr3_4:76164413-76164432	None:intergenic	45.0%
	TGCCCAGATACTTCATAGAG+AGG	-	chr3_4:76164414-76164433	None:intergenic	45.0%
!!	TAAAATTATTTAATTAAATC+TGG	-	chr3_4:76164619-76164638	None:intergenic	5.0%
!!	TAAAATTATTTAATTAAATC+TGG	-	chr3_4:76164619-76164638	None:intergenic	5.0%
!	TGCGTTGGCAATGTTGGACA+AGG	+	chr3_4:76163687-76163706	Msa0522840:CDS	50.0%
	ACAAGGACACTCCTGATCGT+TGG	+	chr3_4:76163704-76163723	Msa0522840:CDS	50.0%
!	TGCGTTGGCAATGTTGGACA+AGG	+	chr3_4:76163687-76163706	Msa0522840:CDS	50.0%
	ACAAGGACACTCCTGATCGT+TGG	+	chr3_4:76163704-76163723	Msa0522840:CDS	50.0%
	CACTCCTGATCGTTGGCAGA+AGG	+	chr3_4:76163711-76163730	Msa0522840:CDS	55.0%
	TCCTGATCGTTGGCAGAAGG+TGG	+	chr3_4:76163714-76163733	Msa0522840:CDS	55.0%
	CAGAAGGTGGCTAGAGCTGT+GGG	+	chr3_4:76163727-76163746	Msa0522840:CDS	55.0%
	CACTCCTGATCGTTGGCAGA+AGG	+	chr3_4:76163711-76163730	Msa0522840:CDS	55.0%
	TCCTGATCGTTGGCAGAAGG+TGG	+	chr3_4:76163714-76163733	Msa0522840:CDS	55.0%
	CAGAAGGTGGCTAGAGCTGT+GGG	+	chr3_4:76163727-76163746	Msa0522840:CDS	55.0%
	GCCACCTTCTGCCAACGATC+AGG	-	chr3_4:76163718-76163737	None:intergenic	60.0%
	GCAGAAGGTGGCTAGAGCTG+TGG	+	chr3_4:76163726-76163745	Msa0522840:CDS	60.0%
	AAGGTGGCTAGAGCTGTGGG+AGG	+	chr3_4:76163730-76163749	Msa0522840:CDS	60.0%
	AGGTGGCTAGAGCTGTGGGA+GGG	+	chr3_4:76163731-76163750	Msa0522840:CDS	60.0%
	GCCACCTTCTGCCAACGATC+AGG	-	chr3_4:76163718-76163737	None:intergenic	60.0%
	GCAGAAGGTGGCTAGAGCTG+TGG	+	chr3_4:76163726-76163745	Msa0522840:CDS	60.0%
	AAGGTGGCTAGAGCTGTGGG+AGG	+	chr3_4:76163730-76163749	Msa0522840:CDS	60.0%
	AGGTGGCTAGAGCTGTGGGA+GGG	+	chr3_4:76163731-76163750	Msa0522840:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	76163573	76164653	76163573	ID=Msa0522840;Name=Msa0522840
chr3_4	mRNA	76163573	76164653	76163573	ID=Msa0522840-mRNA-1;Parent=Msa0522840;Name=Msa0522840-mRNA-1;_AED=0.01;_eAED=0.01;_QI=64\|1\|1\|1\|1\|1\|2\|308\|91
chr3_4	exon	76163573	76163887	76163573	ID=Msa0522840-mRNA-1:exon:21910;Parent=Msa0522840-mRNA-1
chr3_4	exon	76164321	76164653	76164321	ID=Msa0522840-mRNA-1:exon:21911;Parent=Msa0522840-mRNA-1
chr3_4	five_prime_UTR	76163573	76163636	76163573	ID=Msa0522840-mRNA-1:five_prime_utr;Parent=Msa0522840-mRNA-1
chr3_4	CDS	76163637	76163887	76163637	ID=Msa0522840-mRNA-1:cds;Parent=Msa0522840-mRNA-1
chr3_4	CDS	76164321	76164345	76164321	ID=Msa0522840-mRNA-1:cds;Parent=Msa0522840-mRNA-1
chr3_4	three_prime_UTR	76164346	76164653	76164346	ID=Msa0522840-mRNA-1:three_prime_utr;Parent=Msa0522840-mRNA-1

Gene Sequence	>Msa0522840 ATGGCTTCAAGTTCGAATTGGACCGCAAAGCAGAACAAAAGATTTGAGAATGCGTTGGCAATGTTGGACAAGGACACTCCTGATCGTTGGCAGAAGGTGGCTAGAGCTGTGGGAGGGAAGACTGTTGAAGAAGTGAAAAGACATTATGAGAACCTTGTTGAAGATATTAGGCAGATCGAGGAAGGCCATGTTCCCCTGCCTAATTACACAAATAATGTTGGTTACAATTACATAATGGATCAAGATAAAAGGATGAAGGCTCTAAGTCTCCACTAA
Protein sequence	>Msa0522840 MASSSNWTAKQNKRFENALAMLDKDTPDRWQKVARAVGGKTVEEVKRHYENLVEDIRQIEEGHVPLPNYTNNVGYNYIMDQDKRMKALSLH*