AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0441960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0441960 MtrunA17_Chr3g0088851 79.048 105 12 1 8 112 1 95 2.07e-54 168
Msa0441960 MtrunA17_Chr3g0090311 46.667 165 67 6 13 166 2 156 4.00e-32 113
Msa0441960 MtrunA17_Chr3g0098461 40.000 125 64 4 44 166 37 152 6.30e-17 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0441960 AT5G13780.1 43.902 164 71 6 13 165 2 155 2.28e-28 104

Find 55 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACAAGTACTTTGATATTT+TGG 0.170716 3_3:-10697337 Msa0441960:three_prime_UTR
TTCCACGATCTGCCAATATT+CGG 0.311860 3_3:+10699561 None:intergenic
TGTTCCTTGTAACTGTTTCA+TGG 0.332180 3_3:+10699668 None:intergenic
ATGCATACTTCAAAATATTA+AGG 0.338549 3_3:+10697076 None:intergenic
TCCACGATCTGCCAATATTC+GGG 0.342849 3_3:+10699562 None:intergenic
GAGCTGCAGAGTTGCTCTTC+CGG 0.345662 3_3:+10698650 None:intergenic
AATCCTTAACACCTAATCTA+TGG 0.347939 3_3:+10697212 None:intergenic
TCTTGGTTTGTTTGAAAAGA+TGG 0.363394 3_3:-10698629 Msa0441960:CDS
GTTATATCAAATTGTTCATA+AGG 0.378902 3_3:+10697234 None:intergenic
AGAGGAAGAGGAAGAGGAAG+AGG 0.394447 3_3:-10699460 Msa0441960:CDS
AGAGGAAGTGGAAGAGGAAG+AGG 0.396334 3_3:-10699478 Msa0441960:CDS
CTCGTTACTCTCCCGAATAT+TGG 0.398957 3_3:-10699573 Msa0441960:CDS
TAACCATAGATTAGGTGTTA+AGG 0.408808 3_3:-10697215 Msa0441960:three_prime_UTR
AGTGGAAGAGGAAGAGGAAG+AGG 0.420722 3_3:-10699472 Msa0441960:CDS
AACAATACAGCCGCTGCAAT+CGG 0.422612 3_3:+10699518 None:intergenic
ATTTGATATAACCATAGATT+AGG 0.426119 3_3:-10697223 Msa0441960:three_prime_UTR
AGAGGAAGAGGAAGAGGAAG+AGG 0.426997 3_3:-10699466 Msa0441960:CDS
CTCCTCATCATCAAAATACT+TGG 0.430558 3_3:+10698582 None:intergenic
TGCATAAGAGTCGCAACAAT+TGG 0.433948 3_3:-10699627 Msa0441960:CDS
CTCTAAAGAGGAAGTGGAAG+AGG 0.438354 3_3:-10699484 Msa0441960:CDS
GAAGAGGAAGAGGAAGAGGA+AGG 0.438355 3_3:-10699456 Msa0441960:CDS
CGCCCAAAACACCATGGAAA+AGG 0.453353 3_3:-10699352 Msa0441960:intron
CTTGGTTTGTTTGAAAAGAT+GGG 0.459057 3_3:-10698628 Msa0441960:CDS
CCTCCTCTCTAAAGAGGAAG+TGG 0.459131 3_3:-10699490 Msa0441960:CDS
AAGAGCAACTCTGCAGCTCT+TGG 0.462439 3_3:-10698646 Msa0441960:CDS
TGCTTACCTCCTCTCTAAAG+AGG 0.467882 3_3:-10699496 Msa0441960:CDS
CAATTGGAGAAGTATTAGAT+TGG 0.473450 3_3:-10699611 Msa0441960:CDS
AAGATGTCTCGGTGCACGTC+CGG 0.505757 3_3:-10698669 Msa0441960:CDS
AACATGGATTCTCATGCTGC+AGG 0.509412 3_3:-10697438 Msa0441960:three_prime_UTR
CTTCCACTTCCTCTTTAGAG+AGG 0.515368 3_3:+10699487 None:intergenic
GAAGAGGAAGAGGAAGGGAA+TGG 0.522153 3_3:-10699450 Msa0441960:CDS
AAGAGGAAGAGGAAGAGGAA+GGG 0.522871 3_3:-10699455 Msa0441960:CDS
CCAAAATATCAAAGTACTTG+TGG 0.523332 3_3:+10697337 None:intergenic
CCACTTCCTCTTTAGAGAGG+AGG 0.536526 3_3:+10699490 None:intergenic
GAGTGGCTCTCACAGTTAGG+TGG 0.542114 3_3:+10698516 None:intergenic
GATTTAACATGTAGTACTGT+GGG 0.544885 3_3:-10697193 Msa0441960:three_prime_UTR
TTAATTTCAGGTCTCAAACA+TGG 0.547864 3_3:-10697454 Msa0441960:intron
AGGAACAATGTCGTCAAAGA+AGG 0.558119 3_3:-10699652 Msa0441960:CDS
CACGTCGCGGCCGATTGCAG+CGG 0.560201 3_3:-10699528 Msa0441960:CDS
TTACACACTTCTTGAGGTGA+AGG 0.565558 3_3:-10698605 Msa0441960:CDS
ATAGGTATGGTGAACAAAGA+GGG 0.575631 3_3:+10699410 None:intergenic
GATGGGTTACACACTTCTTG+AGG 0.581446 3_3:-10698611 Msa0441960:CDS
AGGAGGACGCTATTGTCTTG+TGG 0.587701 3_3:-10698564 Msa0441960:CDS
TCCCGAATATTGGCAGATCG+TGG 0.596909 3_3:-10699563 Msa0441960:CDS
CTAACCATGAAACAGTTACA+AGG 0.600808 3_3:-10699672 None:intergenic
GATAGGTATGGTGAACAAAG+AGG 0.607709 3_3:+10699409 None:intergenic
GGATTTAACATGTAGTACTG+TGG 0.615437 3_3:-10697194 Msa0441960:three_prime_UTR
CATGGTAAATCGTTAGCTGA+AGG 0.616527 3_3:+10697408 None:intergenic
CACCGCCGCCCAAAACACCA+TGG 0.620119 3_3:-10699358 Msa0441960:CDS
CACCATACCTATCGTAAACG+TGG 0.628725 3_3:-10699399 Msa0441960:CDS
ATGGATTCTCATGCTGCAGG+AGG 0.629432 3_3:-10697435 Msa0441960:three_prime_UTR
GGTGGGTGCTAAAGATGTCT+CGG 0.629911 3_3:-10698680 Msa0441960:CDS
AGTGAGTGCTGAGTAATGAG+AGG 0.650346 3_3:-10697047 Msa0441960:three_prime_UTR
TACCACGTTTACGATAGGTA+TGG 0.651983 3_3:+10699397 None:intergenic
AGCGATACCACGTTTACGAT+AGG 0.681782 3_3:+10699392 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTGTTATAATTTTGTTTTT+TGG - chr3_3:10697402-10697421 Msa0441960:intron 15.0%
!!! ATAATTTTGTTTTTTGGATT+AGG - chr3_3:10697408-10697427 Msa0441960:intron 15.0%
!! GAAAATAATTGAAACTTTGA+TGG - chr3_3:10698253-10698272 Msa0441960:intron 20.0%
!!! TTGATAGTCAATAATACTTA+GGG - chr3_3:10698356-10698375 Msa0441960:intron 20.0%
!! TAATCACAATTCATTAAACA+AGG + chr3_3:10699171-10699190 None:intergenic 20.0%
!!! AAAATATACAAAGTACTACA+TGG + chr3_3:10699326-10699345 None:intergenic 20.0%
!!! ACTTTGTATATTTTCTAGTA+TGG - chr3_3:10699332-10699351 Msa0441960:intron 20.0%
!!! ACTTTGTATATTTTCTAGTA+TGG - chr3_3:10699332-10699351 Msa0441960:intron 20.0%
!!! AAAAATTATCAAAGTACTTG+CGG + chr3_3:10699449-10699468 None:intergenic 20.0%
!! GTTATATCAAATTGTTCATA+AGG + chr3_3:10699482-10699501 None:intergenic 20.0%
!! ATTTGATATAACCATAGATT+AGG - chr3_3:10699490-10699509 Msa0441960:CDS 20.0%
!! ATGCATACTTCAAAATATTA+AGG + chr3_3:10699640-10699659 None:intergenic 20.0%
!! TTTCTTCTTGTTAGTGATTT+TGG - chr3_3:10697782-10697801 Msa0441960:intron 25.0%
! GAAAACCAAAAGAAAAATCT+CGG + chr3_3:10697865-10697884 None:intergenic 25.0%
!!! AGACCTTTTTTTGATGTTTT+TGG - chr3_3:10697954-10697973 Msa0441960:intron 25.0%
! CAACCAAAAACATCAAAAAA+AGG + chr3_3:10697960-10697979 None:intergenic 25.0%
! ACATTGTAACAGACTATTAA+TGG + chr3_3:10697999-10698018 None:intergenic 25.0%
! TCAAGAAAATGGAAAAAGAT+GGG + chr3_3:10698224-10698243 None:intergenic 25.0%
! TTCAAGAAAATGGAAAAAGA+TGG + chr3_3:10698225-10698244 None:intergenic 25.0%
! TCACAAAACATTCAAGAAAA+TGG + chr3_3:10698235-10698254 None:intergenic 25.0%
!! CTTGATAGTCAATAATACTT+AGG - chr3_3:10698355-10698374 Msa0441960:intron 25.0%
! TTAACTACTAGTACCTTATA+AGG - chr3_3:10698719-10698738 Msa0441960:intron 25.0%
! AAATATACTGTTTGAGTATG+TGG + chr3_3:10698791-10698810 None:intergenic 25.0%
! CATTTGTTCTCTTAATTGAT+TGG - chr3_3:10699008-10699027 Msa0441960:intron 25.0%
! GAAAATATGCAAGTAGTAAA+AGG + chr3_3:10699146-10699165 None:intergenic 25.0%
!! AATTAAATCATGGTGTTTTC+TGG - chr3_3:10699234-10699253 Msa0441960:intron 25.0%
!!! TGTTTTCTGGATTTAATTTC+AGG - chr3_3:10699247-10699266 Msa0441960:intron 25.0%
!!! AGTATGGTATCTTTTAAAGT+AGG - chr3_3:10699348-10699367 Msa0441960:intron 25.0%
!!! GTATGGTATCTTTTAAAGTA+GGG - chr3_3:10699349-10699368 Msa0441960:intron 25.0%
!!! TATGGTATCTTTTAAAGTAG+GGG - chr3_3:10699350-10699369 Msa0441960:intron 25.0%
!!! AGTATGGTATCTTTTAAAGT+AGG - chr3_3:10699348-10699367 Msa0441960:intron 25.0%
!!! GTATGGTATCTTTTAAAGTA+GGG - chr3_3:10699349-10699368 Msa0441960:intron 25.0%
!!! AGTTTGGTATCTTTTAAAGT+AGG - chr3_3:10699552-10699571 Msa0441960:CDS 25.0%
!!! GTTTGGTATCTTTTAAAGTA+GGG - chr3_3:10699553-10699572 Msa0441960:CDS 25.0%
!! CAATTGGAGAAGTATTAGAT+TGG - chr3_3:10697102-10697121 Msa0441960:three_prime_UTR 30.0%
TCCATACATAACCAGTTAAA+AGG + chr3_3:10697473-10697492 None:intergenic 30.0%
! TATGTATGGAGTTGGTTTAA+TGG - chr3_3:10697483-10697502 Msa0441960:intron 30.0%
AGAAAACAGAAAATCATCAC+AGG + chr3_3:10697611-10697630 None:intergenic 30.0%
! TGTGATGATTTTCTGTTTTC+TGG - chr3_3:10697610-10697629 Msa0441960:intron 30.0%
! CTAGGATTATCTTTTATTCC+TGG - chr3_3:10697654-10697673 Msa0441960:intron 30.0%
! TTATCTTTTATTCCTGGAAG+TGG - chr3_3:10697660-10697679 Msa0441960:intron 30.0%
!! TTTTCTTGTTTCCTAGTCTT+GGG - chr3_3:10697880-10697899 Msa0441960:intron 30.0%
ACAATGTGTAATTTGACTGA+AGG - chr3_3:10698012-10698031 Msa0441960:intron 30.0%
!! TCTTGGTTTGTTTGAAAAGA+TGG - chr3_3:10698084-10698103 Msa0441960:intron 30.0%
!! CTTGGTTTGTTTGAAAAGAT+GGG - chr3_3:10698085-10698104 Msa0441960:intron 30.0%
AAGAAAATGGAAAAAGATGG+GGG + chr3_3:10698222-10698241 None:intergenic 30.0%
CAAGAAAATGGAAAAAGATG+GGG + chr3_3:10698223-10698242 None:intergenic 30.0%
! ATAATTGAAACTTTGATGGC+TGG - chr3_3:10698257-10698276 Msa0441960:intron 30.0%
CTGGTTTAATATTGACATGA+TGG - chr3_3:10698276-10698295 Msa0441960:intron 30.0%
! TTTTGATCGCTATCTTTTTG+TGG - chr3_3:10698393-10698412 Msa0441960:intron 30.0%
TTATCCAAGGATGAAAAAGT+CGG + chr3_3:10698441-10698460 None:intergenic 30.0%
! TGTAAAGAGCATATTATCCA+AGG + chr3_3:10698454-10698473 None:intergenic 30.0%
TGGATAATATGCTCTTTACA+TGG - chr3_3:10698454-10698473 Msa0441960:intron 30.0%
!! TTTCTTGAGTGTAGTTCTAA+TGG - chr3_3:10698567-10698586 Msa0441960:CDS 30.0%
!! TTCTTGAGTGTAGTTCTAAT+GGG - chr3_3:10698568-10698587 Msa0441960:CDS 30.0%
!! TGTTGCTTATTGATTTCGAA+TGG - chr3_3:10698656-10698675 Msa0441960:CDS 30.0%
TAACGTCATGTAAAGTAACA+CGG + chr3_3:10698702-10698721 None:intergenic 30.0%
TCTAAAAAGTCGTCCTTATA+AGG + chr3_3:10698735-10698754 None:intergenic 30.0%
!! TTTGTCTTTTTCACAGATCT+AGG - chr3_3:10698839-10698858 Msa0441960:intron 30.0%
TTAATCCTTTCAGCTTTCTT+CGG - chr3_3:10698913-10698932 Msa0441960:intron 30.0%
GCTTATTGAGGATTAAGATT+AGG - chr3_3:10699054-10699073 Msa0441960:intron 30.0%
TGCTGATGTCAATTAAATCA+TGG - chr3_3:10699224-10699243 Msa0441960:intron 30.0%
! TTAATTTCAGGTCTCAAACA+TGG - chr3_3:10699259-10699278 Msa0441960:intron 30.0%
! CCAAAATATCAAAGTACTTG+TGG + chr3_3:10699379-10699398 None:intergenic 30.0%
! CCACAAGTACTTTGATATTT+TGG - chr3_3:10699376-10699395 Msa0441960:CDS 30.0%
TAACCATAGATTAGGTGTTA+AGG - chr3_3:10699498-10699517 Msa0441960:CDS 30.0%
AATCCTTAACACCTAATCTA+TGG + chr3_3:10699504-10699523 None:intergenic 30.0%
GATTTAACATGTAGTACTGT+GGG - chr3_3:10699520-10699539 Msa0441960:CDS 30.0%
ATAGGTATGGTGAACAAAGA+GGG + chr3_3:10697306-10697325 None:intergenic 35.0%
! AACCTTTTCCATGGTGTTTT+GGG + chr3_3:10697366-10697385 None:intergenic 35.0%
! TAACCTTTTCCATGGTGTTT+TGG + chr3_3:10697367-10697386 None:intergenic 35.0%
AAAACACCATGGAAAAGGTT+AGG - chr3_3:10697366-10697385 Msa0441960:intron 35.0%
! AACAAACCTAACCTTTTCCA+TGG + chr3_3:10697375-10697394 None:intergenic 35.0%
!! GCCTTTTAACTGGTTATGTA+TGG - chr3_3:10697469-10697488 Msa0441960:intron 35.0%
TAACTGGTTATGTATGGAGT+TGG - chr3_3:10697475-10697494 Msa0441960:intron 35.0%
! ATGGAGTTGGTTTAATGGTT+AGG - chr3_3:10697488-10697507 Msa0441960:intron 35.0%
TAATGGTTAGGTATGAAGCT+TGG - chr3_3:10697500-10697519 Msa0441960:intron 35.0%
AACAAAAAACCTAGCCTACT+CGG + chr3_3:10697554-10697573 None:intergenic 35.0%
! TTTTCAGATAGATCACACAG+TGG + chr3_3:10697588-10697607 None:intergenic 35.0%
! CTTCTTGTTAGTGATTTTGG+TGG - chr3_3:10697785-10697804 Msa0441960:intron 35.0%
!! TTGTTAGTGATTTTGGTGGT+TGG - chr3_3:10697789-10697808 Msa0441960:intron 35.0%
!! TTTGTGTAGTTAGTCTTGTC+TGG - chr3_3:10697823-10697842 Msa0441960:intron 35.0%
AACCAAAAGAAAAATCTCGG+AGG + chr3_3:10697862-10697881 None:intergenic 35.0%
!! GTTTTCTTGTTTCCTAGTCT+TGG - chr3_3:10697879-10697898 Msa0441960:intron 35.0%
!! TTTCTTGTTTCCTAGTCTTG+GGG - chr3_3:10697881-10697900 Msa0441960:intron 35.0%
ATGTGTAATTTGACTGAAGG+TGG - chr3_3:10698015-10698034 Msa0441960:intron 35.0%
TGTGTAATTTGACTGAAGGT+GGG - chr3_3:10698016-10698035 Msa0441960:intron 35.0%
CTCCTCATCATCAAAATACT+TGG + chr3_3:10698134-10698153 None:intergenic 35.0%
!!! CAAGTATTTTGATGATGAGG+AGG - chr3_3:10698132-10698151 Msa0441960:intron 35.0%
ATATTGACATGATGGCAATG+TGG - chr3_3:10698284-10698303 Msa0441960:intron 35.0%
AGTCGGTTAAATACTAGCAT+TGG + chr3_3:10698424-10698443 None:intergenic 35.0%
!!! TTAACCGACTTTTTCATCCT+TGG - chr3_3:10698434-10698453 Msa0441960:intron 35.0%
ATGAGCAATTTAGCTGTGTA+AGG - chr3_3:10698495-10698514 Msa0441960:intron 35.0%
!! TGCTTATTGATTTCGAATGG+AGG - chr3_3:10698659-10698678 Msa0441960:CDS 35.0%
! AAGGACGACTTTTTAGATAG+TGG - chr3_3:10698738-10698757 Msa0441960:intron 35.0%
AAAGCTGAAAGGATTAAAGC+TGG + chr3_3:10698910-10698929 None:intergenic 35.0%
ATAAACCGAAGAAAGCTGAA+AGG + chr3_3:10698921-10698940 None:intergenic 35.0%
! TTTTCTGACTCTGCTCTTAT+TGG - chr3_3:10698941-10698960 Msa0441960:intron 35.0%
ATGTTGCTCATTGCTTATTG+AGG - chr3_3:10699042-10699061 Msa0441960:intron 35.0%
! TGAGGATTAAGATTAGGTTG+TGG - chr3_3:10699060-10699079 Msa0441960:intron 35.0%
!!! AGATTAGGTTGTGGTTTTGT+TGG - chr3_3:10699069-10699088 Msa0441960:intron 35.0%
!!! TGGTATCTTTTAAAGTAGGG+TGG - chr3_3:10699352-10699371 Msa0441960:intron 35.0%
GGATTTAACATGTAGTACTG+TGG - chr3_3:10699519-10699538 Msa0441960:CDS 35.0%
!!! TGGTATCTTTTAAAGTAGGG+TGG - chr3_3:10699352-10699371 Msa0441960:intron 35.0%
AGGAACAATGTCGTCAAAGA+AGG - chr3_3:10697061-10697080 Msa0441960:three_prime_UTR 40.0%
TGCATAAGAGTCGCAACAAT+TGG - chr3_3:10697086-10697105 Msa0441960:three_prime_UTR 40.0%
TTCCACGATCTGCCAATATT+CGG + chr3_3:10697155-10697174 None:intergenic 40.0%
GATAGGTATGGTGAACAAAG+AGG + chr3_3:10697307-10697326 None:intergenic 40.0%
TACCACGTTTACGATAGGTA+TGG + chr3_3:10697319-10697338 None:intergenic 40.0%
! TGGATTAGGTTTGTCACTTG+TGG - chr3_3:10697422-10697441 Msa0441960:three_prime_UTR 40.0%
! GGATTAGGTTTGTCACTTGT+GGG - chr3_3:10697423-10697442 Msa0441960:three_prime_UTR 40.0%
! TGTGGTATAGGCCTTTTAAC+TGG - chr3_3:10697459-10697478 Msa0441960:intron 40.0%
GTTAGGTATGAAGCTTGGTT+CGG - chr3_3:10697505-10697524 Msa0441960:intron 40.0%
!! TTGGTTCGGTTGTTTCGTTA+GGG - chr3_3:10697519-10697538 Msa0441960:intron 40.0%
GGTAGAATCTGAGTTTCACT+AGG - chr3_3:10697631-10697650 Msa0441960:intron 40.0%
AATCTGAGTTTCACTAGGCT+AGG - chr3_3:10697636-10697655 Msa0441960:intron 40.0%
TATTCCTGGAAGTGGTTCTT+TGG - chr3_3:10697668-10697687 Msa0441960:intron 40.0%
AAAGGGAGTGGCAATGATTA+GGG + chr3_3:10697700-10697719 None:intergenic 40.0%
TAAAGGGAGTGGCAATGATT+AGG + chr3_3:10697701-10697720 None:intergenic 40.0%
AAACCAATTCTCTGTGGGAA+GGG - chr3_3:10697734-10697753 Msa0441960:intron 40.0%
! GTCCTCCGAGATTTTTCTTT+TGG - chr3_3:10697857-10697876 Msa0441960:intron 40.0%
AAAATCCATACCCCAAGACT+AGG + chr3_3:10697894-10697913 None:intergenic 40.0%
! TTGGGGTATGGATTTTCTCT+AGG - chr3_3:10697898-10697917 Msa0441960:intron 40.0%
TTACACACTTCTTGAGGTGA+AGG - chr3_3:10698108-10698127 Msa0441960:intron 40.0%
!!! GGCCAAGTATTTTGATGATG+AGG - chr3_3:10698129-10698148 Msa0441960:intron 40.0%
AGCAATTTAGCTGTGTAAGG+TGG - chr3_3:10698498-10698517 Msa0441960:intron 40.0%
GCAATTTAGCTGTGTAAGGT+GGG - chr3_3:10698499-10698518 Msa0441960:intron 40.0%
CAGTGCTTTCTATTTCAGTG+TGG - chr3_3:10698620-10698639 Msa0441960:CDS 40.0%
ACCAGACAGAGCACAATATA+CGG + chr3_3:10698887-10698906 None:intergenic 40.0%
GTTGGACTTAGACTAACACT+TGG - chr3_3:10699087-10699106 Msa0441960:intron 40.0%
ACTTGGAGTAAAAAGTCCTG+AGG - chr3_3:10699104-10699123 Msa0441960:intron 40.0%
AAACCATAGCTATGATCCTC+AGG + chr3_3:10699123-10699142 None:intergenic 40.0%
CATGGTAAATCGTTAGCTGA+AGG + chr3_3:10699308-10699327 None:intergenic 40.0%
!!! GGTATCTTTTAAAGTAGGGG+TGG - chr3_3:10699353-10699372 Msa0441960:CDS 40.0%
!! CTGTGGGATTTTTCGAAGTT+TGG - chr3_3:10699536-10699555 Msa0441960:CDS 40.0%
CTCGTTACTCTCCCGAATAT+TGG - chr3_3:10697140-10697159 Msa0441960:three_prime_UTR 45.0%
TCCACGATCTGCCAATATTC+GGG + chr3_3:10697154-10697173 None:intergenic 45.0%
! GAAAAAGCTTGTTCACGTCG+CGG - chr3_3:10697172-10697191 Msa0441960:three_prime_UTR 45.0%
AACAATACAGCCGCTGCAAT+CGG + chr3_3:10697198-10697217 None:intergenic 45.0%
! TGCTTACCTCCTCTCTAAAG+AGG - chr3_3:10697217-10697236 Msa0441960:three_prime_UTR 45.0%
! CTTCCACTTCCTCTTTAGAG+AGG + chr3_3:10697229-10697248 None:intergenic 45.0%
! CTCTAAAGAGGAAGTGGAAG+AGG - chr3_3:10697229-10697248 Msa0441960:three_prime_UTR 45.0%
AAGAGGAAGAGGAAGAGGAA+GGG - chr3_3:10697246-10697265 Msa0441960:three_prime_UTR 45.0%
CACCATACCTATCGTAAACG+TGG - chr3_3:10697314-10697333 Msa0441960:three_prime_UTR 45.0%
AGCGATACCACGTTTACGAT+AGG + chr3_3:10697324-10697343 None:intergenic 45.0%
!! CTTTTCCATGGTGTTTTGGG+CGG + chr3_3:10697363-10697382 None:intergenic 45.0%
!! TCACTTGTGGGTTAGCGTAT+AGG - chr3_3:10697435-10697454 Msa0441960:three_prime_UTR 45.0%
!! CACTTGTGGGTTAGCGTATA+GGG - chr3_3:10697436-10697455 Msa0441960:three_prime_UTR 45.0%
TAGCGTATAGGGTGTGGTAT+AGG - chr3_3:10697447-10697466 Msa0441960:three_prime_UTR 45.0%
!! CTTGGTTCGGTTGTTTCGTT+AGG - chr3_3:10697518-10697537 Msa0441960:intron 45.0%
!! TGGTTCGGTTGTTTCGTTAG+GGG - chr3_3:10697520-10697539 Msa0441960:intron 45.0%
TTCACCAAAGAACCACTTCC+AGG + chr3_3:10697675-10697694 None:intergenic 45.0%
ATTGCCACTCCCTTTATGAG+TGG - chr3_3:10697705-10697724 Msa0441960:intron 45.0%
TTGCCACTCCCTTTATGAGT+GGG - chr3_3:10697706-10697725 Msa0441960:intron 45.0%
TTTCGCCTCCCACTCATAAA+GGG + chr3_3:10697717-10697736 None:intergenic 45.0%
AGGCGAAACCAATTCTCTGT+GGG - chr3_3:10697729-10697748 Msa0441960:intron 45.0%
GAAACCAATTCTCTGTGGGA+AGG - chr3_3:10697733-10697752 Msa0441960:intron 45.0%
AAACCCTTCCCACAGAGAAT+TGG + chr3_3:10697740-10697759 None:intergenic 45.0%
! TTCTCTGTGGGAAGGGTTTT+TGG - chr3_3:10697741-10697760 Msa0441960:intron 45.0%
!! TGTTTCCTAGTCTTGGGGTA+TGG - chr3_3:10697886-10697905 Msa0441960:intron 45.0%
!! GATGGGTTACACACTTCTTG+AGG - chr3_3:10698102-10698121 Msa0441960:intron 45.0%
AATGGAAAAAGATGGGGGAG+TGG + chr3_3:10698217-10698236 None:intergenic 45.0%
TCCGTATATTGTGCTCTGTC+TGG - chr3_3:10698883-10698902 Msa0441960:intron 45.0%
AGTCCTGAGGATCATAGCTA+TGG - chr3_3:10699117-10699136 Msa0441960:intron 45.0%
AACATGGATTCTCATGCTGC+AGG - chr3_3:10699275-10699294 Msa0441960:intron 45.0%
TCCCGAATATTGGCAGATCG+TGG - chr3_3:10697150-10697169 Msa0441960:three_prime_UTR 50.0%
! CCACTTCCTCTTTAGAGAGG+AGG + chr3_3:10697226-10697245 None:intergenic 50.0%
! CCTCCTCTCTAAAGAGGAAG+TGG - chr3_3:10697223-10697242 Msa0441960:three_prime_UTR 50.0%
AGAGGAAGTGGAAGAGGAAG+AGG - chr3_3:10697235-10697254 Msa0441960:three_prime_UTR 50.0%
AGTGGAAGAGGAAGAGGAAG+AGG - chr3_3:10697241-10697260 Msa0441960:three_prime_UTR 50.0%
AGAGGAAGAGGAAGAGGAAG+AGG - chr3_3:10697247-10697266 Msa0441960:three_prime_UTR 50.0%
AGAGGAAGAGGAAGAGGAAG+AGG - chr3_3:10697247-10697266 Msa0441960:three_prime_UTR 50.0%
GAAGAGGAAGAGGAAGAGGA+AGG - chr3_3:10697245-10697264 Msa0441960:three_prime_UTR 50.0%
GAAGAGGAAGAGGAAGGGAA+TGG - chr3_3:10697263-10697282 Msa0441960:three_prime_UTR 50.0%
CGCCCAAAACACCATGGAAA+AGG - chr3_3:10697361-10697380 Msa0441960:intron 50.0%
! TTCGGTTGTTTCGTTAGGGG+TGG - chr3_3:10697523-10697542 Msa0441960:intron 50.0%
! TCGGTTGTTTCGTTAGGGGT+GGG - chr3_3:10697524-10697543 Msa0441960:intron 50.0%
TTGTTTCGTTAGGGGTGGGA+AGG - chr3_3:10697528-10697547 Msa0441960:intron 50.0%
GTTTCGCCTCCCACTCATAA+AGG + chr3_3:10697718-10697737 None:intergenic 50.0%
GAGGCGAAACCAATTCTCTG+TGG - chr3_3:10697728-10697747 Msa0441960:intron 50.0%
GTCTTGTCTGGTGAGTGTTC+TGG - chr3_3:10697835-10697854 Msa0441960:intron 50.0%
TACAACAGCTGCAGGTCTCA+AGG + chr3_3:10697933-10697952 None:intergenic 50.0%
AAGGTCTGTACAACAGCTGC+AGG + chr3_3:10697941-10697960 None:intergenic 50.0%
GGTGGGTGCTAAAGATGTCT+CGG - chr3_3:10698033-10698052 Msa0441960:intron 50.0%
AAGAGCAACTCTGCAGCTCT+TGG - chr3_3:10698067-10698086 Msa0441960:intron 50.0%
AGGAGGACGCTATTGTCTTG+TGG - chr3_3:10698149-10698168 Msa0441960:intron 50.0%
ATGGATTCTCATGCTGCAGG+AGG - chr3_3:10699278-10699297 Msa0441960:intron 50.0%
!! TTCCATGGTGTTTTGGGCGG+CGG + chr3_3:10697360-10697379 None:intergenic 55.0%
!! GTGGGTTAGCGTATAGGGTG+TGG - chr3_3:10697441-10697460 Msa0441960:three_prime_UTR 55.0%
CCTCCCACTCATAAAGGGAG+TGG + chr3_3:10697712-10697731 None:intergenic 55.0%
CCACTCCCTTTATGAGTGGG+AGG - chr3_3:10697709-10697728 Msa0441960:intron 55.0%
! AAGATGTCTCGGTGCACGTC+CGG - chr3_3:10698044-10698063 Msa0441960:intron 55.0%
GAGCTGCAGAGTTGCTCTTC+CGG + chr3_3:10698066-10698085 None:intergenic 55.0%
GAGTGGCTCTCACAGTTAGG+TGG + chr3_3:10698200-10698219 None:intergenic 55.0%
! GTGGGCGTGAGGTCAGATTT+TGG - chr3_3:10698517-10698536 Msa0441960:intron 55.0%
GGGGAGTGGCTCTCACAGTT+AGG + chr3_3:10698203-10698222 None:intergenic 60.0%
AGCTGTGTAAGGTGGGCGTG+AGG - chr3_3:10698506-10698525 Msa0441960:intron 60.0%
CACCGCCGCCCAAAACACCA+TGG - chr3_3:10697355-10697374 Msa0441960:intron 65.0%
TAGGGGTGGGAAGGCCGAGT+AGG - chr3_3:10697537-10697556 Msa0441960:intron 65.0%
GTGGGAAGGCCGAGTAGGCT+AGG - chr3_3:10697542-10697561 Msa0441960:intron 65.0%
CACGTCGCGGCCGATTGCAG+CGG - chr3_3:10697185-10697204 Msa0441960:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 10697047 10699688 10697047 ID=Msa0441960;Name=Msa0441960
chr3_3 mRNA 10697047 10699688 10697047 ID=Msa0441960-mRNA-1;Parent=Msa0441960;Name=Msa0441960-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|1|0.75|1|0.33|0.25|4|382|168
chr3_3 exon 10697047 10697368 10697047 ID=Msa0441960-mRNA-1:exon:2337;Parent=Msa0441960-mRNA-1
chr3_3 exon 10697409 10697466 10697409 ID=Msa0441960-mRNA-1:exon:2336;Parent=Msa0441960-mRNA-1
chr3_3 exon 10698529 10698701 10698529 ID=Msa0441960-mRNA-1:exon:2335;Parent=Msa0441960-mRNA-1
chr3_3 exon 10699353 10699688 10699353 ID=Msa0441960-mRNA-1:exon:2334;Parent=Msa0441960-mRNA-1
chr3_3 CDS 10699353 10699688 10699353 ID=Msa0441960-mRNA-1:cds;Parent=Msa0441960-mRNA-1
chr3_3 CDS 10698531 10698701 10698531 ID=Msa0441960-mRNA-1:cds;Parent=Msa0441960-mRNA-1
chr3_3 three_prime_UTR 10698529 10698530 10698529 ID=Msa0441960-mRNA-1:three_prime_utr;Parent=Msa0441960-mRNA-1
chr3_3 three_prime_UTR 10697409 10697466 10697409 ID=Msa0441960-mRNA-1:three_prime_utr;Parent=Msa0441960-mRNA-1
chr3_3 three_prime_UTR 10697047 10697368 10697047 ID=Msa0441960-mRNA-1:three_prime_utr;Parent=Msa0441960-mRNA-1
Gene Sequence

>Msa0441960

ATGAAACAGTTACAAGGAACAATGTCGTCAAAGAAGGTGTGCATAAGAGTCGCAACAATTGGAGAAGTATTAGATTGGAACAGTAACAACAATCTCGTTACTCTCCCGAATATTGGCAGATCGTGGAAAAAGCTTGTTCACGTCGCGGCCGATTGCAGCGGCTGTATTGTTGCTTACCTCCTCTCTAAAGAGGAAGTGGAAGAGGAAGAGGAAGAGGAAGAGGAAGAGGAAGGGAATGGTCACTTTTGTATCGCTTCCCTCTTTGTTCACCATACCTATCGTAAACGTGGTATCGCTACTGATCTTATCACCGCCGCCCAAAACACCATGGAAAAGGTGGGTGCTAAAGATGTCTCGGTGCACGTCCGGAAGAGCAACTCTGCAGCTCTTGGTTTGTTTGAAAAGATGGGTTACACACTTCTTGAGGTGAAGGCCAAGTATTTTGATGATGAGGAGGACGCTATTGTCTTGTGGAAGAAACTCAACGAGAAGCATATAGCCACCTAA
Protein sequence

>Msa0441960

MKQLQGTMSSKKVCIRVATIGEVLDWNSNNNLVTLPNIGRSWKKLVHVAADCSGCIVAYLLSKEEVEEEEEEEEEEEGNGHFCIASLFVHHTYRKRGIATDLITAAQNTMEKVGAKDVSVHVRKSNSAALGLFEKMGYTLLEVKAKYFDDEEDAIVLWKKLNEKHIAT*