AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0456060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0456060 MtrunA17_Chr3g0103091 99.537 216 1 0 44 259 1 216 1.83e-161 446
Msa0456060 MtrunA17_Chr5g0441031 73.125 160 20 1 100 259 1 137 6.25e-73 219
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0456060 AT3G13230.2 75.814 215 51 1 44 257 1 215 5.61e-123 349
Msa0456060 AT3G13230.1 75.814 215 51 1 44 257 1 215 5.61e-123 349

Find 66 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTTTGTTACCTAATTA+TGG 0.104484 3_3:+49338195 Msa0456060:CDS
GTTACAGAAATATCAAATTT+TGG 0.188544 3_3:+49338339 Msa0456060:three_prime_UTR
AGATCCCAGAATGTGAATTT+TGG 0.238915 3_3:-49338146 None:intergenic
CTGCTGGTGATCCCATAATT+AGG 0.273715 3_3:-49338207 None:intergenic
TGATACCAAAATTCACATTC+TGG 0.283493 3_3:+49338141 Msa0456060:CDS
AGTTGAGAGCCGTTACTTCT+AGG 0.295245 3_3:+49338245 Msa0456060:CDS
TGCTATTGCTATTCTGCGTT+TGG 0.312006 3_3:+49337970 Msa0456060:CDS
TCTCTTTGTTACCTAATTAT+GGG 0.325990 3_3:+49338196 Msa0456060:CDS
TTTGTTCATGCTTTCATGTT+GGG 0.347801 3_3:+49337932 Msa0456060:CDS
GGCTCAAACTTTGGCTTTGG+AGG 0.352039 3_3:-49337689 None:intergenic
AGAGGCTCAAACTTTGGCTT+TGG 0.356673 3_3:-49337692 None:intergenic
AAAACGTTGATGCATTTGAT+AGG 0.361018 3_3:+49338613 Msa0456060:three_prime_UTR
ATACACCTCTCAAGAAAGTT+TGG 0.369468 3_3:+49337780 Msa0456060:CDS
GATCACTTGTCCCGTGCTAT+TGG 0.373903 3_3:+49338046 Msa0456060:CDS
CGTGCTATTGGAAGATTATC+TGG 0.376662 3_3:+49338058 Msa0456060:CDS
TGCATTCTCAATTGTAAACT+TGG 0.386651 3_3:-49338095 None:intergenic
GGTTTCAGAGGCTCAAACTT+TGG 0.395402 3_3:-49337698 None:intergenic
TTCGACCCTTCAAATTCATT+CGG 0.417898 3_3:-49337847 None:intergenic
TGTTGAGTCCTTTGAGATCA+AGG 0.427401 3_3:+49338003 Msa0456060:CDS
TAAAACAACAATATATATCT+TGG 0.429057 3_3:+49338483 Msa0456060:three_prime_UTR
GGTGCTATGGATTTGAGCTC+TGG 0.432278 3_3:+49338504 Msa0456060:three_prime_UTR
CACTGTTCTCCCTTTCCAAA+GGG 0.463167 3_3:-49338458 None:intergenic
ATTGGAAGATTATCTGGAAA+AGG 0.469137 3_3:+49338064 Msa0456060:CDS
TTGCAAACATCAAGATTGCT+AGG 0.471818 3_3:+49338170 Msa0456060:CDS
TCTCCCTTTCCAAAGGGAAA+AGG 0.475899 3_3:-49338452 None:intergenic
GGAACCTGGACGGACTTTCT+CGG 0.477466 3_3:-49336730 None:intergenic
CAATATATATCTTGGTGCTA+TGG 0.479965 3_3:+49338491 Msa0456060:three_prime_UTR
AGAAGTAACGGCTCTCAACT+TGG 0.482912 3_3:-49338242 None:intergenic
TTTCCTTGTCTTGTACTGAA+AGG 0.486053 3_3:+49338363 Msa0456060:three_prime_UTR
TGACATCCGAATGAATTTGA+AGG 0.495230 3_3:+49337841 Msa0456060:CDS
TTGGAATATACCTTTCCTGC+TGG 0.496163 3_3:-49338223 None:intergenic
CGAAACTGAACCGCAGCACC+AGG 0.498813 3_3:-49337731 None:intergenic
GATACCAAAATTCACATTCT+GGG 0.511425 3_3:+49338142 Msa0456060:CDS
AAACGTTGATGCATTTGATA+GGG 0.511588 3_3:+49338614 Msa0456060:three_prime_UTR
GACTTTCTCGGTGTTTCTTG+CGG 0.514271 3_3:-49336718 None:intergenic
TATTTATGAATGCTTGCTGA+TGG 0.516149 3_3:+49338313 Msa0456060:three_prime_UTR
CTGAAACCTCATGAGATGCC+TGG 0.516679 3_3:+49337713 Msa0456060:CDS
TGCAAACATCAAGATTGCTA+GGG 0.524357 3_3:+49338171 Msa0456060:CDS
AAGGATGTTAAGACACTTCG+AGG 0.530319 3_3:+49338022 Msa0456060:CDS
CTAATTATGGGATCACCAGC+AGG 0.540701 3_3:+49338208 Msa0456060:CDS
TATGGGATCACCAGCAGGAA+AGG 0.547596 3_3:+49338213 Msa0456060:CDS
CAGATAATCTTCCAATAGCA+CGG 0.551311 3_3:-49338057 None:intergenic
GACATCCGAATGAATTTGAA+GGG 0.559638 3_3:+49337842 Msa0456060:CDS
CTCTTTAAACAGTTGCACAA+TGG 0.561554 3_3:+49337616 Msa0456060:intron
ACCTCTCAAGAAAGTTTGGA+TGG 0.563023 3_3:+49337784 Msa0456060:CDS
TCACTGTTCTCCCTTTCCAA+AGG 0.565955 3_3:-49338459 None:intergenic
TCATGAGATGCCTGGTGCTG+CGG 0.567566 3_3:+49337721 Msa0456060:CDS
TCCATCCAAACTTTCTTGAG+AGG 0.572045 3_3:-49337785 None:intergenic
GGAAGATTATCTGGAAAAGG+TGG 0.573573 3_3:+49338067 Msa0456060:CDS
TTCTGCCATCCTAGAAGTAA+CGG 0.576020 3_3:-49338254 None:intergenic
GAGAGCCGTTACTTCTAGGA+TGG 0.578516 3_3:+49338249 Msa0456060:CDS
TTACTTCTAGGATGGCAGAA+AGG 0.584575 3_3:+49338257 Msa0456060:CDS
CACAATGGCATCATCTACAA+TGG 0.590257 3_3:+49337631 Msa0456060:CDS
AGATAATCTTCCAATAGCAC+GGG 0.591480 3_3:-49338056 None:intergenic
AACACCGAGAAAGTCCGTCC+AGG 0.601247 3_3:+49336726 Msa0456060:CDS
TGCTGCGGTTCAGTTTCGCA+AGG 0.601400 3_3:+49337736 Msa0456060:CDS
CTTAACATCCTTGATCTCAA+AGG 0.606154 3_3:-49338011 None:intergenic
GGCATCTCATGAGGTTTCAG+AGG 0.608176 3_3:-49337710 None:intergenic
GCACTCAACTCAACTGCCGT+CGG 0.632641 3_3:+49336669 Msa0456060:CDS
TGGTGACTTAAATACTAACA+TGG 0.643332 3_3:+49338524 Msa0456060:three_prime_UTR
GCAGCACCAGGCATCTCATG+AGG 0.646562 3_3:-49337719 None:intergenic
GACGGCAGTTGAGTTGAGTG+CGG 0.649450 3_3:-49336667 None:intergenic
AATCCTTTCAGTACAAGACA+AGG 0.656921 3_3:-49338366 None:intergenic
TTGAGAGGTGTATAACGATG+TGG 0.685518 3_3:-49337770 None:intergenic
GAAAAGTTGAGTTGAAGACA+AGG 0.702510 3_3:+49337867 Msa0456060:CDS
AGAGGTGTATAACGATGTGG+TGG 0.702702 3_3:-49337767 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTAAATTTCATTATG+CGG + chr3_3:49337141-49337160 Msa0456060:intron 10.0%
!!! AAATTTTAAATTTCATTATG+CGG + chr3_3:49337141-49337160 Msa0456060:intron 10.0%
!!! AATTTTAAATTTCATTATGC+GGG + chr3_3:49337142-49337161 Msa0456060:intron 15.0%
!! TATAAACAGAATACTTATAT+AGG - chr3_3:49337286-49337305 None:intergenic 15.0%
!! AAATGTTTCAAAATTATGAA+TGG + chr3_3:49337339-49337358 Msa0456060:intron 15.0%
!! AATGTTTCAAAATTATGAAT+GGG + chr3_3:49337340-49337359 Msa0456060:intron 15.0%
!! TAAAACAACAATATATATCT+TGG + chr3_3:49338483-49338502 Msa0456060:three_prime_UTR 15.0%
!!! AATTTTAAATTTCATTATGC+GGG + chr3_3:49337142-49337161 Msa0456060:intron 15.0%
!! TATAAACAGAATACTTATAT+AGG - chr3_3:49337286-49337305 None:intergenic 15.0%
!! AAATGTTTCAAAATTATGAA+TGG + chr3_3:49337339-49337358 Msa0456060:intron 15.0%
!! AATGTTTCAAAATTATGAAT+GGG + chr3_3:49337340-49337359 Msa0456060:intron 15.0%
!! TAAAACAACAATATATATCT+TGG + chr3_3:49338483-49338502 Msa0456060:three_prime_UTR 15.0%
!! AAAGCCAAAATTAAGAAAAA+GGG - chr3_3:49336755-49336774 None:intergenic 20.0%
!! AAAAGCCAAAATTAAGAAAA+AGG - chr3_3:49336756-49336775 None:intergenic 20.0%
!! TAAAAAGCAAGCTAATTAAT+TGG - chr3_3:49336933-49336952 None:intergenic 20.0%
!! TTATTCTCTTAGTTTATCAT+TGG + chr3_3:49336957-49336976 Msa0456060:intron 20.0%
!! TATTCTCTTAGTTTATCATT+GGG + chr3_3:49336958-49336977 Msa0456060:intron 20.0%
!!! GTTACAGAAATATCAAATTT+TGG + chr3_3:49338339-49338358 Msa0456060:three_prime_UTR 20.0%
!! AAAGCCAAAATTAAGAAAAA+GGG - chr3_3:49336755-49336774 None:intergenic 20.0%
!! AAAAGCCAAAATTAAGAAAA+AGG - chr3_3:49336756-49336775 None:intergenic 20.0%
!! TAAAAAGCAAGCTAATTAAT+TGG - chr3_3:49336933-49336952 None:intergenic 20.0%
!! TTATTCTCTTAGTTTATCAT+TGG + chr3_3:49336957-49336976 Msa0456060:intron 20.0%
!! TATTCTCTTAGTTTATCATT+GGG + chr3_3:49336958-49336977 Msa0456060:intron 20.0%
!!! GTTACAGAAATATCAAATTT+TGG + chr3_3:49338339-49338358 Msa0456060:three_prime_UTR 20.0%
! AAGCCAAAATTAAGAAAAAG+GGG - chr3_3:49336754-49336773 None:intergenic 25.0%
! ATTCTCTTAGTTTATCATTG+GGG + chr3_3:49336959-49336978 Msa0456060:intron 25.0%
! AGTTTCTATCAAAACAAACA+AGG - chr3_3:49337001-49337020 None:intergenic 25.0%
!!! TTTTTCGAAAAACATTCATG+TGG + chr3_3:49337032-49337051 Msa0456060:intron 25.0%
! TCAAAACAATTGTTGCAAAA+AGG - chr3_3:49337093-49337112 None:intergenic 25.0%
! ATGTTCTGATTAAAAAGACA+TGG + chr3_3:49337171-49337190 Msa0456060:intron 25.0%
! ATGTGTTACATACATATATG+AGG + chr3_3:49337417-49337436 Msa0456060:intron 25.0%
!! AATTTTCATCTGCTCAAATA+CGG - chr3_3:49337822-49337841 None:intergenic 25.0%
! TTCTCTTTGTTACCTAATTA+TGG + chr3_3:49338195-49338214 Msa0456060:CDS 25.0%
! TCTCTTTGTTACCTAATTAT+GGG + chr3_3:49338196-49338215 Msa0456060:CDS 25.0%
! AAGCCAAAATTAAGAAAAAG+GGG - chr3_3:49336754-49336773 None:intergenic 25.0%
! ATTCTCTTAGTTTATCATTG+GGG + chr3_3:49336959-49336978 Msa0456060:intron 25.0%
! AGTTTCTATCAAAACAAACA+AGG - chr3_3:49337001-49337020 None:intergenic 25.0%
!!! TTTTTCGAAAAACATTCATG+TGG + chr3_3:49337032-49337051 Msa0456060:intron 25.0%
! TCAAAACAATTGTTGCAAAA+AGG - chr3_3:49337093-49337112 None:intergenic 25.0%
! ATGTTCTGATTAAAAAGACA+TGG + chr3_3:49337171-49337190 Msa0456060:intron 25.0%
! ATGTGTTACATACATATATG+AGG + chr3_3:49337417-49337436 Msa0456060:intron 25.0%
!! AATTTTCATCTGCTCAAATA+CGG - chr3_3:49337822-49337841 None:intergenic 25.0%
! TTCTCTTTGTTACCTAATTA+TGG + chr3_3:49338195-49338214 Msa0456060:CDS 25.0%
! TCTCTTTGTTACCTAATTAT+GGG + chr3_3:49338196-49338215 Msa0456060:CDS 25.0%
! GTTCCCCTTTTTCTTAATTT+TGG + chr3_3:49336748-49336767 Msa0456060:intron 30.0%
GCAAGCTAATTAATTGGTTA+CGG - chr3_3:49336927-49336946 None:intergenic 30.0%
AACAATTGTTGCAAAAAGGT+AGG - chr3_3:49337089-49337108 None:intergenic 30.0%
TCTGATTAAAAAGACATGGA+AGG + chr3_3:49337175-49337194 Msa0456060:intron 30.0%
AGCTGATACTGAAATTATAG+AGG + chr3_3:49337313-49337332 Msa0456060:intron 30.0%
! GCAAAACTTTTTGTTCAGAT+AGG - chr3_3:49337670-49337689 None:intergenic 30.0%
! ATTTTCATCTGCTCAAATAC+GGG - chr3_3:49337821-49337840 None:intergenic 30.0%
! TTTTGTTCATGCTTTCATGT+TGG + chr3_3:49337931-49337950 Msa0456060:CDS 30.0%
TTTGTTCATGCTTTCATGTT+GGG + chr3_3:49337932-49337951 Msa0456060:CDS 30.0%
ATTGGAAGATTATCTGGAAA+AGG + chr3_3:49338064-49338083 Msa0456060:CDS 30.0%
TGCATTCTCAATTGTAAACT+TGG - chr3_3:49338098-49338117 None:intergenic 30.0%
TGATACCAAAATTCACATTC+TGG + chr3_3:49338141-49338160 Msa0456060:CDS 30.0%
GATACCAAAATTCACATTCT+GGG + chr3_3:49338142-49338161 Msa0456060:CDS 30.0%
! TATTTATGAATGCTTGCTGA+TGG + chr3_3:49338313-49338332 Msa0456060:three_prime_UTR 30.0%
!! CAATATATATCTTGGTGCTA+TGG + chr3_3:49338491-49338510 Msa0456060:three_prime_UTR 30.0%
TGGTGACTTAAATACTAACA+TGG + chr3_3:49338524-49338543 Msa0456060:three_prime_UTR 30.0%
AAAACGTTGATGCATTTGAT+AGG + chr3_3:49338613-49338632 Msa0456060:three_prime_UTR 30.0%
AAACGTTGATGCATTTGATA+GGG + chr3_3:49338614-49338633 Msa0456060:three_prime_UTR 30.0%
! GTTCCCCTTTTTCTTAATTT+TGG + chr3_3:49336748-49336767 Msa0456060:intron 30.0%
GCAAGCTAATTAATTGGTTA+CGG - chr3_3:49336927-49336946 None:intergenic 30.0%
AACAATTGTTGCAAAAAGGT+AGG - chr3_3:49337089-49337108 None:intergenic 30.0%
TCTGATTAAAAAGACATGGA+AGG + chr3_3:49337175-49337194 Msa0456060:intron 30.0%
AGCTGATACTGAAATTATAG+AGG + chr3_3:49337313-49337332 Msa0456060:intron 30.0%
! GCAAAACTTTTTGTTCAGAT+AGG - chr3_3:49337670-49337689 None:intergenic 30.0%
! ATTTTCATCTGCTCAAATAC+GGG - chr3_3:49337821-49337840 None:intergenic 30.0%
! TTTTGTTCATGCTTTCATGT+TGG + chr3_3:49337931-49337950 Msa0456060:CDS 30.0%
TTTGTTCATGCTTTCATGTT+GGG + chr3_3:49337932-49337951 Msa0456060:CDS 30.0%
ATTGGAAGATTATCTGGAAA+AGG + chr3_3:49338064-49338083 Msa0456060:CDS 30.0%
TGCATTCTCAATTGTAAACT+TGG - chr3_3:49338098-49338117 None:intergenic 30.0%
TGATACCAAAATTCACATTC+TGG + chr3_3:49338141-49338160 Msa0456060:CDS 30.0%
GATACCAAAATTCACATTCT+GGG + chr3_3:49338142-49338161 Msa0456060:CDS 30.0%
! TATTTATGAATGCTTGCTGA+TGG + chr3_3:49338313-49338332 Msa0456060:three_prime_UTR 30.0%
!! CAATATATATCTTGGTGCTA+TGG + chr3_3:49338491-49338510 Msa0456060:three_prime_UTR 30.0%
TGGTGACTTAAATACTAACA+TGG + chr3_3:49338524-49338543 Msa0456060:three_prime_UTR 30.0%
AAAACGTTGATGCATTTGAT+AGG + chr3_3:49338613-49338632 Msa0456060:three_prime_UTR 30.0%
AATTAAGAAAAAGGGGAACC+TGG - chr3_3:49336747-49336766 None:intergenic 35.0%
! ATTGTAATTTGCGCTGGAAT+CGG - chr3_3:49336812-49336831 None:intergenic 35.0%
TCGAAAAACATTCATGTGGA+TGG + chr3_3:49337036-49337055 Msa0456060:intron 35.0%
TTGTTGCAAAAAGGTAGGTA+GGG - chr3_3:49337084-49337103 None:intergenic 35.0%
ATTGTTGCAAAAAGGTAGGT+AGG - chr3_3:49337085-49337104 None:intergenic 35.0%
ACATGGAAGGATCATACTAA+TGG + chr3_3:49337188-49337207 Msa0456060:intron 35.0%
GACACACTCAGTAAATAAGA+TGG + chr3_3:49337253-49337272 Msa0456060:intron 35.0%
!! ACAAAAGTGCTAATAGCTGT+AGG + chr3_3:49337477-49337496 Msa0456060:intron 35.0%
ATAGTTCAGGGAACATGTTA+TGG - chr3_3:49337521-49337540 None:intergenic 35.0%
ACATGTTCCCTGAACTATTT+AGG + chr3_3:49337523-49337542 Msa0456060:intron 35.0%
TCTACCACCTAAATAGTTCA+GGG - chr3_3:49337533-49337552 None:intergenic 35.0%
ATCTACCACCTAAATAGTTC+AGG - chr3_3:49337534-49337553 None:intergenic 35.0%
CTCTTTAAACAGTTGCACAA+TGG + chr3_3:49337616-49337635 Msa0456060:intron 35.0%
ATACACCTCTCAAGAAAGTT+TGG + chr3_3:49337780-49337799 Msa0456060:CDS 35.0%
TGACATCCGAATGAATTTGA+AGG + chr3_3:49337841-49337860 Msa0456060:CDS 35.0%
GACATCCGAATGAATTTGAA+GGG + chr3_3:49337842-49337861 Msa0456060:CDS 35.0%
TTCGACCCTTCAAATTCATT+CGG - chr3_3:49337850-49337869 None:intergenic 35.0%
!! GAAAAGTTGAGTTGAAGACA+AGG + chr3_3:49337867-49337886 Msa0456060:CDS 35.0%
! CAAAATCAGCACATTTTTGC+AGG - chr3_3:49337916-49337935 None:intergenic 35.0%
CTTAACATCCTTGATCTCAA+AGG - chr3_3:49338014-49338033 None:intergenic 35.0%
AGATAATCTTCCAATAGCAC+GGG - chr3_3:49338059-49338078 None:intergenic 35.0%
CAGATAATCTTCCAATAGCA+CGG - chr3_3:49338060-49338079 None:intergenic 35.0%
! AGATCCCAGAATGTGAATTT+TGG - chr3_3:49338149-49338168 None:intergenic 35.0%
TTGCAAACATCAAGATTGCT+AGG + chr3_3:49338170-49338189 Msa0456060:CDS 35.0%
TGCAAACATCAAGATTGCTA+GGG + chr3_3:49338171-49338190 Msa0456060:CDS 35.0%
TTTCCTTGTCTTGTACTGAA+AGG + chr3_3:49338363-49338382 Msa0456060:three_prime_UTR 35.0%
AATCCTTTCAGTACAAGACA+AGG - chr3_3:49338369-49338388 None:intergenic 35.0%
!! AATTCCTTTTCCCTTTGGAA+AGG + chr3_3:49338448-49338467 Msa0456060:three_prime_UTR 35.0%
!! ATTCCTTTTCCCTTTGGAAA+GGG + chr3_3:49338449-49338468 Msa0456060:three_prime_UTR 35.0%
AATTAAGAAAAAGGGGAACC+TGG - chr3_3:49336747-49336766 None:intergenic 35.0%
! ATTGTAATTTGCGCTGGAAT+CGG - chr3_3:49336812-49336831 None:intergenic 35.0%
TCGAAAAACATTCATGTGGA+TGG + chr3_3:49337036-49337055 Msa0456060:intron 35.0%
TTGTTGCAAAAAGGTAGGTA+GGG - chr3_3:49337084-49337103 None:intergenic 35.0%
ATTGTTGCAAAAAGGTAGGT+AGG - chr3_3:49337085-49337104 None:intergenic 35.0%
ACATGGAAGGATCATACTAA+TGG + chr3_3:49337188-49337207 Msa0456060:intron 35.0%
GACACACTCAGTAAATAAGA+TGG + chr3_3:49337253-49337272 Msa0456060:intron 35.0%
!! ACAAAAGTGCTAATAGCTGT+AGG + chr3_3:49337477-49337496 Msa0456060:intron 35.0%
ATAGTTCAGGGAACATGTTA+TGG - chr3_3:49337521-49337540 None:intergenic 35.0%
ACATGTTCCCTGAACTATTT+AGG + chr3_3:49337523-49337542 Msa0456060:intron 35.0%
TCTACCACCTAAATAGTTCA+GGG - chr3_3:49337533-49337552 None:intergenic 35.0%
ATCTACCACCTAAATAGTTC+AGG - chr3_3:49337534-49337553 None:intergenic 35.0%
CTCTTTAAACAGTTGCACAA+TGG + chr3_3:49337616-49337635 Msa0456060:intron 35.0%
ATACACCTCTCAAGAAAGTT+TGG + chr3_3:49337780-49337799 Msa0456060:CDS 35.0%
TGACATCCGAATGAATTTGA+AGG + chr3_3:49337841-49337860 Msa0456060:CDS 35.0%
GACATCCGAATGAATTTGAA+GGG + chr3_3:49337842-49337861 Msa0456060:CDS 35.0%
TTCGACCCTTCAAATTCATT+CGG - chr3_3:49337850-49337869 None:intergenic 35.0%
!! GAAAAGTTGAGTTGAAGACA+AGG + chr3_3:49337867-49337886 Msa0456060:CDS 35.0%
! CAAAATCAGCACATTTTTGC+AGG - chr3_3:49337916-49337935 None:intergenic 35.0%
CTTAACATCCTTGATCTCAA+AGG - chr3_3:49338014-49338033 None:intergenic 35.0%
AGATAATCTTCCAATAGCAC+GGG - chr3_3:49338059-49338078 None:intergenic 35.0%
CAGATAATCTTCCAATAGCA+CGG - chr3_3:49338060-49338079 None:intergenic 35.0%
! AGATCCCAGAATGTGAATTT+TGG - chr3_3:49338149-49338168 None:intergenic 35.0%
TTGCAAACATCAAGATTGCT+AGG + chr3_3:49338170-49338189 Msa0456060:CDS 35.0%
TGCAAACATCAAGATTGCTA+GGG + chr3_3:49338171-49338190 Msa0456060:CDS 35.0%
TTTCCTTGTCTTGTACTGAA+AGG + chr3_3:49338363-49338382 Msa0456060:three_prime_UTR 35.0%
AATCCTTTCAGTACAAGACA+AGG - chr3_3:49338369-49338388 None:intergenic 35.0%
!! AATTCCTTTTCCCTTTGGAA+AGG + chr3_3:49338448-49338467 Msa0456060:three_prime_UTR 35.0%
!! ATTCCTTTTCCCTTTGGAAA+GGG + chr3_3:49338449-49338468 Msa0456060:three_prime_UTR 35.0%
! CACACAATTGTAATTTGCGC+TGG - chr3_3:49336818-49336837 None:intergenic 40.0%
! GTTACGGCTTTTCATCAAAC+AGG - chr3_3:49336911-49336930 None:intergenic 40.0%
TGTTCCCTGAACTATTTAGG+TGG + chr3_3:49337526-49337545 Msa0456060:intron 40.0%
CACAATGGCATCATCTACAA+TGG + chr3_3:49337631-49337650 Msa0456060:CDS 40.0%
! TTGAGAGGTGTATAACGATG+TGG - chr3_3:49337773-49337792 None:intergenic 40.0%
ACCTCTCAAGAAAGTTTGGA+TGG + chr3_3:49337784-49337803 Msa0456060:CDS 40.0%
TCCATCCAAACTTTCTTGAG+AGG - chr3_3:49337788-49337807 None:intergenic 40.0%
! TTTTGCAGGTTACTGATGTC+AGG - chr3_3:49337902-49337921 None:intergenic 40.0%
! TGCTATTGCTATTCTGCGTT+TGG + chr3_3:49337970-49337989 Msa0456060:CDS 40.0%
!! TGTTGAGTCCTTTGAGATCA+AGG + chr3_3:49338003-49338022 Msa0456060:CDS 40.0%
AAGGATGTTAAGACACTTCG+AGG + chr3_3:49338022-49338041 Msa0456060:CDS 40.0%
! CGTGCTATTGGAAGATTATC+TGG + chr3_3:49338058-49338077 Msa0456060:CDS 40.0%
GGAAGATTATCTGGAAAAGG+TGG + chr3_3:49338067-49338086 Msa0456060:CDS 40.0%
TTGGAATATACCTTTCCTGC+TGG - chr3_3:49338226-49338245 None:intergenic 40.0%
TTCTGCCATCCTAGAAGTAA+CGG - chr3_3:49338257-49338276 None:intergenic 40.0%
!! TTACTTCTAGGATGGCAGAA+AGG + chr3_3:49338257-49338276 Msa0456060:CDS 40.0%
! CTGTCAATTCCTTTTCCCTT+TGG + chr3_3:49338443-49338462 Msa0456060:three_prime_UTR 40.0%
! CACACAATTGTAATTTGCGC+TGG - chr3_3:49336818-49336837 None:intergenic 40.0%
! GTTACGGCTTTTCATCAAAC+AGG - chr3_3:49336911-49336930 None:intergenic 40.0%
TGTTCCCTGAACTATTTAGG+TGG + chr3_3:49337526-49337545 Msa0456060:intron 40.0%
CACAATGGCATCATCTACAA+TGG + chr3_3:49337631-49337650 Msa0456060:CDS 40.0%
! TTGAGAGGTGTATAACGATG+TGG - chr3_3:49337773-49337792 None:intergenic 40.0%
ACCTCTCAAGAAAGTTTGGA+TGG + chr3_3:49337784-49337803 Msa0456060:CDS 40.0%
TCCATCCAAACTTTCTTGAG+AGG - chr3_3:49337788-49337807 None:intergenic 40.0%
! TTTTGCAGGTTACTGATGTC+AGG - chr3_3:49337902-49337921 None:intergenic 40.0%
! TGCTATTGCTATTCTGCGTT+TGG + chr3_3:49337970-49337989 Msa0456060:CDS 40.0%
!! TGTTGAGTCCTTTGAGATCA+AGG + chr3_3:49338003-49338022 Msa0456060:CDS 40.0%
AAGGATGTTAAGACACTTCG+AGG + chr3_3:49338022-49338041 Msa0456060:CDS 40.0%
! CGTGCTATTGGAAGATTATC+TGG + chr3_3:49338058-49338077 Msa0456060:CDS 40.0%
GGAAGATTATCTGGAAAAGG+TGG + chr3_3:49338067-49338086 Msa0456060:CDS 40.0%
TTGGAATATACCTTTCCTGC+TGG - chr3_3:49338226-49338245 None:intergenic 40.0%
TTCTGCCATCCTAGAAGTAA+CGG - chr3_3:49338257-49338276 None:intergenic 40.0%
!! TTACTTCTAGGATGGCAGAA+AGG + chr3_3:49338257-49338276 Msa0456060:CDS 40.0%
! CTGTCAATTCCTTTTCCCTT+TGG + chr3_3:49338443-49338462 Msa0456060:three_prime_UTR 40.0%
! GTTTTGTGGCAGACACAACT+TGG + chr3_3:49336632-49336651 Msa0456060:CDS 45.0%
! ACTCCTGCTTCTTTTTCCGA+CGG - chr3_3:49336688-49336707 None:intergenic 45.0%
!! GACTTTCTCGGTGTTTCTTG+CGG - chr3_3:49336721-49336740 None:intergenic 45.0%
AAGAAAAAGGGGAACCTGGA+CGG - chr3_3:49336743-49336762 None:intergenic 45.0%
! AGAGGCTCAAACTTTGGCTT+TGG - chr3_3:49337695-49337714 None:intergenic 45.0%
GGTTTCAGAGGCTCAAACTT+TGG - chr3_3:49337701-49337720 None:intergenic 45.0%
! AGAGGTGTATAACGATGTGG+TGG - chr3_3:49337770-49337789 None:intergenic 45.0%
CTGCTGGTGATCCCATAATT+AGG - chr3_3:49338210-49338229 None:intergenic 45.0%
CTAATTATGGGATCACCAGC+AGG + chr3_3:49338208-49338227 Msa0456060:CDS 45.0%
AGAAGTAACGGCTCTCAACT+TGG - chr3_3:49338245-49338264 None:intergenic 45.0%
AGTTGAGAGCCGTTACTTCT+AGG + chr3_3:49338245-49338264 Msa0456060:CDS 45.0%
TCTCCCTTTCCAAAGGGAAA+AGG - chr3_3:49338455-49338474 None:intergenic 45.0%
CACTGTTCTCCCTTTCCAAA+GGG - chr3_3:49338461-49338480 None:intergenic 45.0%
TCACTGTTCTCCCTTTCCAA+AGG - chr3_3:49338462-49338481 None:intergenic 45.0%
! GTTTTGTGGCAGACACAACT+TGG + chr3_3:49336632-49336651 Msa0456060:CDS 45.0%
! ACTCCTGCTTCTTTTTCCGA+CGG - chr3_3:49336688-49336707 None:intergenic 45.0%
!! GACTTTCTCGGTGTTTCTTG+CGG - chr3_3:49336721-49336740 None:intergenic 45.0%
AAGAAAAAGGGGAACCTGGA+CGG - chr3_3:49336743-49336762 None:intergenic 45.0%
! AGAGGCTCAAACTTTGGCTT+TGG - chr3_3:49337695-49337714 None:intergenic 45.0%
GGTTTCAGAGGCTCAAACTT+TGG - chr3_3:49337701-49337720 None:intergenic 45.0%
! AGAGGTGTATAACGATGTGG+TGG - chr3_3:49337770-49337789 None:intergenic 45.0%
CTGCTGGTGATCCCATAATT+AGG - chr3_3:49338210-49338229 None:intergenic 45.0%
CTAATTATGGGATCACCAGC+AGG + chr3_3:49338208-49338227 Msa0456060:CDS 45.0%
AGAAGTAACGGCTCTCAACT+TGG - chr3_3:49338245-49338264 None:intergenic 45.0%
AGTTGAGAGCCGTTACTTCT+AGG + chr3_3:49338245-49338264 Msa0456060:CDS 45.0%
TCTCCCTTTCCAAAGGGAAA+AGG - chr3_3:49338455-49338474 None:intergenic 45.0%
CACTGTTCTCCCTTTCCAAA+GGG - chr3_3:49338461-49338480 None:intergenic 45.0%
TCACTGTTCTCCCTTTCCAA+AGG - chr3_3:49338462-49338481 None:intergenic 45.0%
TCGGATCATTGCGTGCTCAT+CGG - chr3_3:49336793-49336812 None:intergenic 50.0%
!! GGCTCAAACTTTGGCTTTGG+AGG - chr3_3:49337692-49337711 None:intergenic 50.0%
GGCATCTCATGAGGTTTCAG+AGG - chr3_3:49337713-49337732 None:intergenic 50.0%
CTGAAACCTCATGAGATGCC+TGG + chr3_3:49337713-49337732 Msa0456060:CDS 50.0%
GATCACTTGTCCCGTGCTAT+TGG + chr3_3:49338046-49338065 Msa0456060:CDS 50.0%
TATGGGATCACCAGCAGGAA+AGG + chr3_3:49338213-49338232 Msa0456060:CDS 50.0%
! GAGAGCCGTTACTTCTAGGA+TGG + chr3_3:49338249-49338268 Msa0456060:CDS 50.0%
! GGTGCTATGGATTTGAGCTC+TGG + chr3_3:49338504-49338523 Msa0456060:three_prime_UTR 50.0%
TCGGATCATTGCGTGCTCAT+CGG - chr3_3:49336793-49336812 None:intergenic 50.0%
!! GGCTCAAACTTTGGCTTTGG+AGG - chr3_3:49337692-49337711 None:intergenic 50.0%
GGCATCTCATGAGGTTTCAG+AGG - chr3_3:49337713-49337732 None:intergenic 50.0%
CTGAAACCTCATGAGATGCC+TGG + chr3_3:49337713-49337732 Msa0456060:CDS 50.0%
GATCACTTGTCCCGTGCTAT+TGG + chr3_3:49338046-49338065 Msa0456060:CDS 50.0%
TATGGGATCACCAGCAGGAA+AGG + chr3_3:49338213-49338232 Msa0456060:CDS 50.0%
! GAGAGCCGTTACTTCTAGGA+TGG + chr3_3:49338249-49338268 Msa0456060:CDS 50.0%
! GGTGCTATGGATTTGAGCTC+TGG + chr3_3:49338504-49338523 Msa0456060:three_prime_UTR 50.0%
!! GACGGCAGTTGAGTTGAGTG+CGG - chr3_3:49336670-49336689 None:intergenic 55.0%
GCACTCAACTCAACTGCCGT+CGG + chr3_3:49336669-49336688 Msa0456060:CDS 55.0%
CTGCCGTCGGAAAAAGAAGC+AGG + chr3_3:49336682-49336701 Msa0456060:CDS 55.0%
AACACCGAGAAAGTCCGTCC+AGG + chr3_3:49336726-49336745 Msa0456060:CDS 55.0%
! GGAACCTGGACGGACTTTCT+CGG - chr3_3:49336733-49336752 None:intergenic 55.0%
ATTGCGTGCTCATCGGGAGA+GGG - chr3_3:49336786-49336805 None:intergenic 55.0%
CGGATCATTGCGTGCTCATC+GGG - chr3_3:49336792-49336811 None:intergenic 55.0%
! TCATGAGATGCCTGGTGCTG+CGG + chr3_3:49337721-49337740 Msa0456060:CDS 55.0%
TGCTGCGGTTCAGTTTCGCA+AGG + chr3_3:49337736-49337755 Msa0456060:CDS 55.0%
!! GACGGCAGTTGAGTTGAGTG+CGG - chr3_3:49336670-49336689 None:intergenic 55.0%
GCACTCAACTCAACTGCCGT+CGG + chr3_3:49336669-49336688 Msa0456060:CDS 55.0%
CTGCCGTCGGAAAAAGAAGC+AGG + chr3_3:49336682-49336701 Msa0456060:CDS 55.0%
AACACCGAGAAAGTCCGTCC+AGG + chr3_3:49336726-49336745 Msa0456060:CDS 55.0%
! GGAACCTGGACGGACTTTCT+CGG - chr3_3:49336733-49336752 None:intergenic 55.0%
ATTGCGTGCTCATCGGGAGA+GGG - chr3_3:49336786-49336805 None:intergenic 55.0%
CGGATCATTGCGTGCTCATC+GGG - chr3_3:49336792-49336811 None:intergenic 55.0%
! TCATGAGATGCCTGGTGCTG+CGG + chr3_3:49337721-49337740 Msa0456060:CDS 55.0%
TGCTGCGGTTCAGTTTCGCA+AGG + chr3_3:49337736-49337755 Msa0456060:CDS 55.0%
TTGCGTGCTCATCGGGAGAG+GGG - chr3_3:49336785-49336804 None:intergenic 60.0%
CATTGCGTGCTCATCGGGAG+AGG - chr3_3:49336787-49336806 None:intergenic 60.0%
GCAGCACCAGGCATCTCATG+AGG - chr3_3:49337722-49337741 None:intergenic 60.0%
CGAAACTGAACCGCAGCACC+AGG - chr3_3:49337734-49337753 None:intergenic 60.0%
TTGCGTGCTCATCGGGAGAG+GGG - chr3_3:49336785-49336804 None:intergenic 60.0%
CATTGCGTGCTCATCGGGAG+AGG - chr3_3:49336787-49336806 None:intergenic 60.0%
GCAGCACCAGGCATCTCATG+AGG - chr3_3:49337722-49337741 None:intergenic 60.0%
CGAAACTGAACCGCAGCACC+AGG - chr3_3:49337734-49337753 None:intergenic 60.0%
TGCGTGCTCATCGGGAGAGG+GGG - chr3_3:49336784-49336803 None:intergenic 65.0%
TGCGTGCTCATCGGGAGAGG+GGG - chr3_3:49336784-49336803 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 49336626 49338636 49336626 ID=Msa0456060;Name=Msa0456060
chr3_3 mRNA 49336626 49338636 49336626 ID=Msa0456060-mRNA-1;Parent=Msa0456060;Name=Msa0456060-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0.5|1|1|0.5|2|351|259
chr3_3 exon 49336626 49336747 49336626 ID=Msa0456060-mRNA-1:exon:9300;Parent=Msa0456060-mRNA-1
chr3_3 exon 49337628 49338636 49337628 ID=Msa0456060-mRNA-1:exon:9301;Parent=Msa0456060-mRNA-1
chr3_3 CDS 49336626 49336747 49336626 ID=Msa0456060-mRNA-1:cds;Parent=Msa0456060-mRNA-1
chr3_3 CDS 49337628 49338285 49337628 ID=Msa0456060-mRNA-1:cds;Parent=Msa0456060-mRNA-1
chr3_3 three_prime_UTR 49338286 49338636 49338286 ID=Msa0456060-mRNA-1:three_prime_utr;Parent=Msa0456060-mRNA-1
Gene Sequence

>Msa0456060

TTTGAGGTTTTGTGGCAGACACAACTTGGAGTTGCTCTTCGCCGCACTCAACTCAACTGCCGTCGGAAAAAGAAGCAGGAGTCGCTCTTCGTCCGCAAGAAACACCGAGAAAGTCCGTCCAGTTGCACAATGGCATCATCTACAATGGAAGTTGACAATGTCCTATCTGAACAAAAAGTTTTGCCTCCAAAGCCAAAGTTTGAGCCTCTGAAACCTCATGAGATGCCTGGTGCTGCGGTTCAGTTTCGCAAGGTATCAGTTCCACCACATCGTTATACACCTCTCAAGAAAGTTTGGATGGACATCTACACTCCCGTATTTGAGCAGATGAAAATTGACATCCGAATGAATTTGAAGGGTCGAAAAGTTGAGTTGAAGACAAGGCATGATACACCTGACATCAGTAACCTGCAAAAATGTGCTGATTTTGTTCATGCTTTCATGTTGGGTTTTGATGTCATAGATGCTATTGCTATTCTGCGTTTGGATGAGCTCTATGTTGAGTCCTTTGAGATCAAGGATGTTAAGACACTTCGAGGTGATCACTTGTCCCGTGCTATTGGAAGATTATCTGGAAAAGGTGGTAAAACCAAGTTTACAATTGAGAATGCATCAAAGACAAGAATCGTGATTGCTGATACCAAAATTCACATTCTGGGATCTTTTGCAAACATCAAGATTGCTAGGGATTCTCTTTGTTACCTAATTATGGGATCACCAGCAGGAAAGGTATATTCCAAGTTGAGAGCCGTTACTTCTAGGATGGCAGAAAGGTTTTGA
Protein sequence

>Msa0456060

FEVLWQTQLGVALRRTQLNCRRKKKQESLFVRKKHRESPSSCTMASSTMEVDNVLSEQKVLPPKPKFEPLKPHEMPGAAVQFRKVSVPPHRYTPLKKVWMDIYTPVFEQMKIDIRMNLKGRKVELKTRHDTPDISNLQKCADFVHAFMLGFDVIDAIAILRLDELYVESFEIKDVKTLRGDHLSRAIGRLSGKGGKTKFTIENASKTRIVIADTKIHILGSFANIKIARDSLCYLIMGSPAGKVYSKLRAVTSRMAERF*