AlfalfaGEDB | Msa0461420

M. sativa cultivar zhongmu-4 / Msa0461420

KEGG: K00924|K04733
GO: GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0461420	XP_024634954.1	74.895	239	55	3	371	606	22	258	5.49e-115	358
Msa0461420	XP_024634954.1	80.217	369	65	2	1	365	1	365	0.0	596

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0461420	sp\|Q65XV8\|RK176_ORYSJ	58.438	320	129	1	48	363	42	361	6.12e-132	394
Msa0461420	sp\|Q65XV8\|RK176_ORYSJ	58.065	217	89	2	390	606	42	256	1.30e-82	267

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0461420	A0A072UYN9	68.163	245	73	3	365	606	20	262	1.49e-101	323
Msa0461420	A0A072UYN9	71.858	366	99	1	1	362	1	366	5.81e-179	521

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0257580	Msa0461420	0.801226	9.949503e-49	-8.615850e-47
Msa0461420	Msa0511620	0.800274	1.556482e-48	-8.615850e-47
Msa0461420	Msa0745070	0.804333	2.271995e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0461420	MtrunA17_Chr3g0111631	71.858	366	99	1	1	362	1	366	0.0	521
Msa0461420	MtrunA17_Chr3g0111631	68.163	245	73	3	365	606	20	262	3.82e-105	323
Msa0461420	MtrunA17_Chr5g0435211	62.432	370	131	4	3	365	1	369	7.64e-158	459
Msa0461420	MtrunA17_Chr5g0435211	56.180	267	108	3	340	606	4	261	3.03e-93	293
Msa0461420	MtrunA17_Chr3g0111581	65.651	361	112	4	1	357	1	353	5.36e-156	451
Msa0461420	MtrunA17_Chr3g0111581	64.854	239	72	4	371	603	19	251	1.50e-97	301
Msa0461420	MtrunA17_Chr2g0328171	56.944	360	151	1	3	358	1	360	2.12e-141	416
Msa0461420	MtrunA17_Chr2g0328171	57.317	246	100	3	364	606	17	260	4.35e-87	276
Msa0461420	MtrunA17_Chr7g0238671	53.385	384	172	3	3	382	1	381	2.98e-134	398
Msa0461420	MtrunA17_Chr7g0238671	61.187	219	83	2	388	606	43	259	4.24e-86	274

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0461420	AT1G07570.1	53.930	369	160	3	3	367	1	363	1.13e-130	389
Msa0461420	AT1G07570.1	53.360	253	108	4	356	606	10	254	2.63e-82	265
Msa0461420	AT1G07570.5	53.930	369	160	3	3	367	1	363	1.13e-130	389
Msa0461420	AT1G07570.5	53.360	253	108	4	356	606	10	254	2.63e-82	265
Msa0461420	AT1G07570.2	53.930	369	160	3	3	367	1	363	1.13e-130	389
Msa0461420	AT1G07570.2	53.360	253	108	4	356	606	10	254	2.63e-82	265
Msa0461420	AT2G28930.3	54.133	375	166	2	3	373	1	373	3.60e-130	388
Msa0461420	AT2G28930.3	62.617	214	78	2	393	606	47	258	1.12e-84	271
Msa0461420	AT5G02290.2	53.444	363	153	4	3	358	1	354	3.63e-130	387
Msa0461420	AT5G02290.2	59.912	227	79	4	388	606	32	254	1.51e-82	265

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Find 131 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCAAATACCAAAATC+CGG	0.473012	3_3:-59657781	None:intergenic
AAACCAGTACCTGGTTTAGT+AGG	0.485853	3_3:-59654788	None:intergenic
AAACCAGTACCTGGTTTAGT+AGG	0.490695	3_3:-59657514	None:intergenic
AAACCTGAGTATATAGACAC+AGG	0.674523	3_3:+59655563	Msa0461420:CDS
AAAGGATGTTGGAAGAGAAG+AGG	0.547627	3_3:+59657285	Msa0461420:CDS
AAGGGTCTTGCATTTCTTCA+CGG	0.448202	3_3:+59658026	Msa0461420:CDS
ACACTCACTCCTACTAAACC+AGG	0.626208	3_3:+59657505	Msa0461420:CDS
ACCTATGTCTTTATATACTC+AGG	0.552704	3_3:-59658300	None:intergenic
ACCTGAGTATATAAAGACAT+AGG	0.656991	3_3:+59658299	Msa0461420:CDS
ACGCTAGCTCCTACTAAACC+AGG	0.629859	3_3:+59654779	Msa0461420:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAATAAGTCTGTATAAGCA+AGG	-	chr3_3:59654117-59654136	None:intergenic	30.0%
GTTTCTATAATAACATGGAC+AGG	+	chr3_3:59654521-59654540	Msa0461420:intron	30.0%
TGTGAAACACTTCAAATTGT+TGG	-	chr3_3:59654660-59654679	None:intergenic	30.0%
CTAATTAAACCATGTACTTC+AGG	+	chr3_3:59654985-59655004	Msa0461420:intron	30.0%
TCATATCTACCTTGTGTTTA+TGG	+	chr3_3:59655204-59655223	Msa0461420:intron	30.0%
TAATATGTCCGACTATGATA+TGG	+	chr3_3:59655514-59655533	Msa0461420:CDS	30.0%
AATCTTGTTCAAGCCAATAT+TGG	+	chr3_3:59655592-59655611	Msa0461420:intron	30.0%
AAAATACCACATCACATATG+CGG	-	chr3_3:59655689-59655708	None:intergenic	30.0%
AAAAAAAGGCAATGGGAAAA+AGG	-	chr3_3:59655939-59655958	None:intergenic	30.0%
CTTCACAAAAAAAAGGCAAT+GGG	-	chr3_3:59655946-59655965	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	59654099	59658320	59654099	ID=Msa0461420;Name=Msa0461420
chr3_3	mRNA	59654099	59658320	59654099	ID=Msa0461420-mRNA-1;Parent=Msa0461420;Name=Msa0461420-mRNA-1;_AED=0.39;_eAED=0.39;_QI=141\|0.55\|0.5\|1\|1\|1\|10\|0\|606
chr3_3	exon	59654099	59654297	59654099	ID=Msa0461420-mRNA-1:exon:12382;Parent=Msa0461420-mRNA-1
chr3_3	exon	59654543	59654868	59654543	ID=Msa0461420-mRNA-1:exon:12383;Parent=Msa0461420-mRNA-1
chr3_3	exon	59655007	59655142	59655007	ID=Msa0461420-mRNA-1:exon:12384;Parent=Msa0461420-mRNA-1
chr3_3	exon	59655232	59655374	59655232	ID=Msa0461420-mRNA-1:exon:12385;Parent=Msa0461420-mRNA-1
chr3_3	exon	59655461	59655584	59655461	ID=Msa0461420-mRNA-1:exon:12386;Parent=Msa0461420-mRNA-1
chr3_3	exon	59655966	59656271	59655966	ID=Msa0461420-mRNA-1:exon:12387;Parent=Msa0461420-mRNA-1
chr3_3	exon	59657275	59657594	59657275	ID=Msa0461420-mRNA-1:exon:12388;Parent=Msa0461420-mRNA-1
chr3_3	exon	59657704	59657839	59657704	ID=Msa0461420-mRNA-1:exon:12389;Parent=Msa0461420-mRNA-1
chr3_3	exon	59657964	59658106	59657964	ID=Msa0461420-mRNA-1:exon:12390;Parent=Msa0461420-mRNA-1
chr3_3	exon	59658192	59658320	59658192	ID=Msa0461420-mRNA-1:exon:12391;Parent=Msa0461420-mRNA-1
chr3_3	five_prime_UTR	59654099	59654239	59654099	ID=Msa0461420-mRNA-1:five_prime_utr;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59654240	59654297	59654240	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59654543	59654868	59654543	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59655007	59655142	59655007	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59655232	59655374	59655232	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59655461	59655584	59655461	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59655966	59656271	59655966	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59657275	59657594	59657275	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59657704	59657839	59657704	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59657964	59658106	59657964	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1
chr3_3	CDS	59658192	59658320	59658192	ID=Msa0461420-mRNA-1:cds;Parent=Msa0461420-mRNA-1

Gene Sequence

>Msa0461420

ATGTTCATGGCTTGCTGCTTTAGTACAAGGGTCAAGGTTGAGGGTCCTACACATAATGGTTTGAATTCAAAGGTTGATGGAAGAGAAGAGAATGTTTTGAGTGGTTTGAGCAACAAGGAATCCTCTTCAACTGTTCTGCTAACTTCAAGGACTGAGGGTGAGATGTTACAATCCAACAATTTGAAGTGTTTCACATTCAATGAGCTAAAAACTGCTACAAGAAACTTTCGTCCAACTACTGTAGTGGGTGAAGGTACGTTTGGTCGCATCTTTAAGGGGCGGATTGATGAGCACACGCTAGCTCCTACTAAACCAGGTACTGGTTTTGATATTGCTGTGAAGATGTTTAGCCAAGAGAGCAACGAGGGACATAGTGAATGGTTGGTAAAAATCAATTACCTTCGGCAGCTGCGTCATCCTAATCTCGTGAAACTGATTGGTTACTGCTTAGAGGATCCCTATCGGATTTTAGTATATGAATTTTTTACCGAGGGGAGTTTGGATTATCATTTGTTTAAAAGGGTTTCTAACTTTGAACCACTCTCTTGGAATATCCGAATGAAGATAGCTCTTGATGCTGCCAAGGGTCTTGCCTTTCTTCACAGTGATGAAGTGGAAAAGATACATGGAGACTTCAAAACTTCGAAAATCTTGATTGATTCAAACTACAATGCAAAACTATCCTTTTTTGGGTTTTTAAAAGGTAGATCAGATAGTAATATGTCCGACTATGATATGGTGGATATGGGCCCTCAAATTAACGCGAAACCTGAGTATATAGACACAGATCACCTAACCAAAGAAGGCGATGTATACAATTTTGGGGTTGTTCTACTGGAAATTATGTCTGGCAAACGTGCAGTGGATAAAAACAGGCCACTTCGGGAACAAAATTTAGTCGAATGGGCCAAGCCATTACTCAACCGCAAACGTAAAATCTCTCAAGTCATGGATGTTCGTATAGAAGGCCAATACTCTTCGCGCGAAGCAATGAAATTAGCTCATATTGTAATTCAATGTCTGTCTGAAAGACCAAAAATAGAAGAGATCGTGAGATCATTGGAGAAACTTCAAGATTCCAATGACACAGTTGGTTGGAATTCAAAGGATGTTGGAAGAGAAGAGGATGTTTTGAATGGCTTAAACATCAAGGTATTATGTTCATCAACTTCAAGTACTGAGGGTGAGATATTACAATCCAACAATTTGAAGTGTTTCACATTCAATGAGGTAAAAATTGCTATAAGAAATTTTCATCCAAGTGGTCAAGTCGTCGATGAACATGGGTTTGGTAAGGTATTTAAGGGGTGGATCGATGAGCACACACTCACTCCTACTAAACCAGGTACTGGTTTTGTTGTTGCTGTGATGATGTTTAACAAAGAAAGCAACCAGGGACATAGTGAATGGCTGGCAGAAATCAATTACCTTGGGCAGTTGCGTCATCCTAACCTCGTGAAACTGATTGGTTACTGCTTAGAGAATGACTGCCGGATTTTGGTATTTGAATTTTTAGCCAAAGGAAGTTTGGATAATCATTTGTTTAGAAGGGCTTCATACTTTCAGTCACTCTCATGGAAAATCCGTGTGCAGATAGCTCTTCATGCTGCTAAGGGTCTTGCATTTCTTCACGGTGATGAAGTGGAAAGGATACATGGAGACTTCAAAACTTCGAAAATCTTGATTGATTCAAACTACAATGCAAAACTATCCTTTTTTGGGTTGGCAAAATATAGATCAGATAGTAATATGTCCGACTTCGATATGGTGGATATGGACTCAGCTCAAATTAACACAGCACCTGAGTATATAAAGACATAG

Protein sequence

>Msa0461420

MFMACCFSTRVKVEGPTHNGLNSKVDGREENVLSGLSNKESSSTVLLTSRTEGEMLQSNNLKCFTFNELKTATRNFRPTTVVGEGTFGRIFKGRIDEHTLAPTKPGTGFDIAVKMFSQESNEGHSEWLVKINYLRQLRHPNLVKLIGYCLEDPYRILVYEFFTEGSLDYHLFKRVSNFEPLSWNIRMKIALDAAKGLAFLHSDEVEKIHGDFKTSKILIDSNYNAKLSFFGFLKGRSDSNMSDYDMVDMGPQINAKPEYIDTDHLTKEGDVYNFGVVLLEIMSGKRAVDKNRPLREQNLVEWAKPLLNRKRKISQVMDVRIEGQYSSREAMKLAHIVIQCLSERPKIEEIVRSLEKLQDSNDTVGWNSKDVGREEDVLNGLNIKVLCSSTSSTEGEILQSNNLKCFTFNEVKIAIRNFHPSGQVVDEHGFGKVFKGWIDEHTLTPTKPGTGFVVAVMMFNKESNQGHSEWLAEINYLGQLRHPNLVKLIGYCLENDCRILVFEFLAKGSLDNHLFRRASYFQSLSWKIRVQIALHAAKGLAFLHGDEVERIHGDFKTSKILIDSNYNAKLSFFGLAKYRSDSNMSDFDMVDMDSAQINTAPEYIKT*