AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0464030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464030 MtrunA17_Chr3g0114601 98.661 224 3 0 1 224 1 224 4.22e-166 456
Msa0464030 MtrunA17_Chr5g0420141 35.795 176 100 4 61 224 62 236 4.44e-30 111
Msa0464030 MtrunA17_Chr4g0061701 34.545 165 100 3 67 224 59 222 1.17e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464030 AT1G44760.1 58.850 226 73 6 3 224 4 213 8.88e-83 246
Msa0464030 AT1G44760.2 58.850 226 73 6 3 224 75 284 2.07e-81 244
Msa0464030 AT2G03720.2 38.122 181 99 5 56 224 53 232 1.14e-30 113
Msa0464030 AT2G03720.1 39.157 166 90 4 69 224 1 165 1.42e-29 108
Msa0464030 AT1G69080.1 32.222 180 101 3 66 224 44 223 1.96e-28 107
Msa0464030 AT5G17390.1 36.310 168 97 3 67 224 118 285 2.59e-26 103
Msa0464030 AT1G69080.2 34.706 170 96 5 66 224 44 209 5.26e-26 100
Msa0464030 AT3G03290.1 34.503 171 96 4 67 224 107 274 1.51e-23 95.5
Msa0464030 AT4G13450.1 25.728 206 105 4 66 224 15 219 7.86e-16 73.9

Find 82 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAACTCATGTTGTTAATA+AGG 0.212222 3_3:-63598096 Msa0464030:CDS
GCAGAAGAACTTCTGGCTTT+TGG 0.227711 3_3:-63596952 Msa0464030:CDS
CATTTGTGGTTGCATAAATA+AGG 0.255308 3_3:-63598392 Msa0464030:five_prime_UTR
AGATTATTACTTACTATAGA+TGG 0.287966 3_3:+63598489 None:intergenic
ATTCTAAGCATGCAATGATT+TGG 0.290584 3_3:-63598121 Msa0464030:CDS
CTCATCACTGTGGCCAGTTT+AGG 0.329869 3_3:+63597559 None:intergenic
ACTTACCTTGTTAATCATCT+TGG 0.331349 3_3:-63598011 Msa0464030:CDS
AGAAAGAAGATGGCTTCTTT+TGG 0.334209 3_3:+63597497 None:intergenic
GATGAGCCAGGTAAAGAAAT+TGG 0.339137 3_3:-63597542 Msa0464030:CDS
CCAGGTGGCAGAAGAACTTC+TGG 0.346204 3_3:-63596959 Msa0464030:CDS
CTTACCTTGTTAATCATCTT+GGG 0.346303 3_3:-63598010 Msa0464030:CDS
TAAAGTGTCACGGGAATCTT+TGG 0.346325 3_3:+63598597 None:intergenic
TTCTAAGCATGCAATGATTT+GGG 0.371229 3_3:-63598120 Msa0464030:CDS
ACATACAACAACAATAGTTT+AGG 0.386222 3_3:-63598266 Msa0464030:CDS
GGGTTTAACATTAACATGTA+TGG 0.388232 3_3:-63598209 Msa0464030:CDS
CTTGGAAATCAACATCAAAT+AGG 0.391956 3_3:-63598304 Msa0464030:CDS
AAGAGAAGCCAAGGCAATAA+TGG 0.394438 3_3:-63596996 Msa0464030:CDS
AGCCATCTTCTTTCTTTAGC+TGG 0.402605 3_3:-63597489 Msa0464030:intron
AGCAATAATAGTTACATTTG+TGG 0.408337 3_3:-63598406 Msa0464030:five_prime_UTR
AGAGTTATGGTTGTGGTTGA+TGG 0.409544 3_3:-63598152 Msa0464030:CDS
TGTAATTCAAGGACCTAAAC+TGG 0.413139 3_3:-63597572 Msa0464030:CDS
TTATGGGTAAGATTATTCTC+TGG 0.421825 3_3:-63596670 Msa0464030:three_prime_UTR
CAGCAGCACAGAGGAGTTTG+TGG 0.433556 3_3:-63597063 Msa0464030:CDS
CTAACTCATGTTGTTAATAA+GGG 0.434772 3_3:-63598095 Msa0464030:CDS
TCTGCCACCTGGTGCTGATA+AGG 0.435349 3_3:+63596970 None:intergenic
AATGCTGAGTGCTTGACAAT+AGG 0.444116 3_3:-63597026 Msa0464030:CDS
TAGCTGAAAGAGGGAGTGTA+GGG 0.448424 3_3:+63598436 None:intergenic
TGAGACAAATCTGAGTCAAA+TGG 0.450642 3_3:-63598234 Msa0464030:CDS
TAAGGTAGCCATTATTGCCT+TGG 0.456289 3_3:+63596988 None:intergenic
TAAGACTCAGAAGCACTTTG+AGG 0.458252 3_3:+63598041 None:intergenic
AAAATATTATTCACTAGCAT+TGG 0.460639 3_3:+63596701 None:intergenic
AGTGTAGCAGAGGTTCAGTT+TGG 0.464785 3_3:+63598465 None:intergenic
ATAGCTGAAAGAGGGAGTGT+AGG 0.471533 3_3:+63598435 None:intergenic
TATGGGTAAGATTATTCTCT+GGG 0.477840 3_3:-63596669 Msa0464030:three_prime_UTR
GAGAGAGAGATGACATGTGA+GGG 0.485365 3_3:+63598535 None:intergenic
TGGCTTGCAATCTTTGCAAA+GGG 0.487254 3_3:+63597986 None:intergenic
ATTCACTAGCATTGGCAAGT+TGG 0.496266 3_3:+63596709 None:intergenic
GAAATTGGAGGTCTCTATCC+TGG 0.499739 3_3:-63597527 Msa0464030:CDS
GTGGAAGCACTTGTAATTCA+AGG 0.506797 3_3:-63597583 Msa0464030:CDS
AGACCTCCAATTTCTTTACC+TGG 0.507576 3_3:+63597536 None:intergenic
CCAGAAGTTCTTCTGCCACC+TGG 0.508759 3_3:+63596959 None:intergenic
TGGCCTGTCATAGGTGGAAG+TGG 0.514126 3_3:-63597602 Msa0464030:intron
TTTGAGGCAGTTAAGTTCAA+AGG 0.525241 3_3:-63598330 Msa0464030:CDS
CTGGCTTGCAATCTTTGCAA+AGG 0.528141 3_3:+63597985 None:intergenic
TGAAGAGAGTTGTGGAATGA+TGG 0.529080 3_3:-63598183 Msa0464030:CDS
GATTATTACTTACTATAGAT+GGG 0.534676 3_3:+63598490 None:intergenic
GAGGTCTCTATCCTGGTACT+TGG 0.534821 3_3:-63597520 Msa0464030:CDS
TGTGCAGGTTAAAGTGTCAC+GGG 0.538496 3_3:+63598588 None:intergenic
TACCAGCTAAAGAAAGAAGA+TGG 0.539360 3_3:+63597487 None:intergenic
AGTTAAGTTCAAAGGAGACT+TGG 0.544950 3_3:-63598322 Msa0464030:CDS
TTGTGCAGGTTAAAGTGTCA+CGG 0.549553 3_3:+63598587 None:intergenic
GCTAGTTGAATAGCTGAAAG+AGG 0.552730 3_3:+63598426 None:intergenic
AGATGAGAATACAAATATCA+AGG 0.553739 3_3:+63596860 None:intergenic
GATGGTGAGAAAGAGAGTTA+TGG 0.559684 3_3:-63598165 Msa0464030:CDS
AGAGAGAGAGATGACATGTG+AGG 0.561615 3_3:+63598534 None:intergenic
ACTGGCCACAGTGATGAGCC+AGG 0.573013 3_3:-63597554 Msa0464030:CDS
TCAACATCAAATAGGTGGAG+TGG 0.576570 3_3:-63598296 Msa0464030:CDS
GAGAGAGATGACATGTGAGG+GGG 0.576854 3_3:+63598537 None:intergenic
AGAGAGAGATGACATGTGAG+GGG 0.582282 3_3:+63598536 None:intergenic
ACAAATCTGAGTCAAATGGA+AGG 0.584700 3_3:-63598230 Msa0464030:CDS
TACTAGTCTTAGATCTCAAG+AGG 0.588812 3_3:-63596905 Msa0464030:three_prime_UTR
CTGTTTGTTTACAGTCTCTG+TGG 0.592127 3_3:-63597101 Msa0464030:intron
GAATAATCTTACCCATAACA+TGG 0.598325 3_3:+63596675 None:intergenic
GGAGTGAGGAAGAGAAGCCA+AGG 0.601340 3_3:-63597005 Msa0464030:CDS
CTAGTTGAATAGCTGAAAGA+GGG 0.601553 3_3:+63598427 None:intergenic
CAAATCTGAGTCAAATGGAA+GGG 0.609381 3_3:-63598229 Msa0464030:CDS
GGAAATCAACATCAAATAGG+TGG 0.613048 3_3:-63598301 Msa0464030:CDS
CAGCAGCTGCAGCAGCACAG+AGG 0.618197 3_3:-63597072 Msa0464030:CDS
ATGGCTACCTTATCAGCACC+AGG 0.619844 3_3:-63596977 Msa0464030:CDS
TATGGAAATGAAGAGAGTTG+TGG 0.622956 3_3:-63598191 Msa0464030:CDS
GAGCCAGGTAAAGAAATTGG+AGG 0.635543 3_3:-63597539 Msa0464030:CDS
TTGCAAAGATTGCAAGCCAG+AGG 0.640746 3_3:-63597982 Msa0464030:intron
GAGAAAGAGAGTTATGGTTG+TGG 0.642404 3_3:-63598159 Msa0464030:CDS
AATAATCTTACCCATAACAT+GGG 0.644060 3_3:+63596676 None:intergenic
AGGGAGCTAGAGTGTAGCAG+AGG 0.656163 3_3:+63598455 None:intergenic
ACTAGTCTTAGATCTCAAGA+GGG 0.657277 3_3:-63596904 Msa0464030:three_prime_UTR
CTTTACCTGGCTCATCACTG+TGG 0.657425 3_3:+63597549 None:intergenic
GGGACCCAAGATGATTAACA+AGG 0.679967 3_3:+63598006 None:intergenic
AGTGCTTGACAATAGGAGTG+AGG 0.688153 3_3:-63597019 Msa0464030:CDS
GGTTGCATAAATAAGGCACA+AGG 0.702121 3_3:-63598385 Msa0464030:five_prime_UTR
ATGGGTAAGATTATTCTCTG+GGG 0.725595 3_3:-63596668 Msa0464030:three_prime_UTR
GCTACCTTATCAGCACCAGG+TGG 0.765926 3_3:-63596974 Msa0464030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGTAGGAAAATAATTTA+TGG + chr3_3:63597420-63597439 None:intergenic 15.0%
!!! TTTTTTAAAACAAGACAATT+GGG + chr3_3:63597977-63597996 None:intergenic 15.0%
!!! ATTTTTTAAAACAAGACAAT+TGG + chr3_3:63597978-63597997 None:intergenic 15.0%
!! AAAAGTAGGAAAATAATTTA+TGG + chr3_3:63597420-63597439 None:intergenic 15.0%
!!! TTTTTTAAAACAAGACAATT+GGG + chr3_3:63597977-63597996 None:intergenic 15.0%
!!! ATTTTTTAAAACAAGACAAT+TGG + chr3_3:63597978-63597997 None:intergenic 15.0%
!! GATTATTACTTACTATAGAT+GGG + chr3_3:63596703-63596722 None:intergenic 20.0%
!! AGATTATTACTTACTATAGA+TGG + chr3_3:63596704-63596723 None:intergenic 20.0%
!!! TTGTTCAATTGATAGTTAAT+TGG - chr3_3:63597332-63597351 Msa0464030:intron 20.0%
!! ATTAGTTGAAAAGAGAAAAA+GGG + chr3_3:63597387-63597406 None:intergenic 20.0%
!! AATTAGTTGAAAAGAGAAAA+AGG + chr3_3:63597388-63597407 None:intergenic 20.0%
!! AATTTACTATTGTCAATTAG+AGG + chr3_3:63597918-63597937 None:intergenic 20.0%
!!! TTTTTAAAACAAGACAATTG+GGG + chr3_3:63597976-63597995 None:intergenic 20.0%
!!! TAATAAGTCTTGTCATTTTA+AGG - chr3_3:63598419-63598438 Msa0464030:five_prime_UTR 20.0%
!! AAAATATTATTCACTAGCAT+TGG + chr3_3:63598492-63598511 None:intergenic 20.0%
!!! TAATATTTTTACCCATGTTA+TGG - chr3_3:63598503-63598522 Msa0464030:five_prime_UTR 20.0%
!!! AATATTTTTACCCATGTTAT+GGG - chr3_3:63598504-63598523 Msa0464030:five_prime_UTR 20.0%
!! GATTATTACTTACTATAGAT+GGG + chr3_3:63596703-63596722 None:intergenic 20.0%
!! AGATTATTACTTACTATAGA+TGG + chr3_3:63596704-63596723 None:intergenic 20.0%
!!! TTGTTCAATTGATAGTTAAT+TGG - chr3_3:63597332-63597351 Msa0464030:intron 20.0%
!! ATTAGTTGAAAAGAGAAAAA+GGG + chr3_3:63597387-63597406 None:intergenic 20.0%
!! AATTAGTTGAAAAGAGAAAA+AGG + chr3_3:63597388-63597407 None:intergenic 20.0%
!! AATTTACTATTGTCAATTAG+AGG + chr3_3:63597918-63597937 None:intergenic 20.0%
!!! TTTTTAAAACAAGACAATTG+GGG + chr3_3:63597976-63597995 None:intergenic 20.0%
!!! TAATAAGTCTTGTCATTTTA+AGG - chr3_3:63598419-63598438 Msa0464030:five_prime_UTR 20.0%
!! AAAATATTATTCACTAGCAT+TGG + chr3_3:63598492-63598511 None:intergenic 20.0%
!!! TAATATTTTTACCCATGTTA+TGG - chr3_3:63598503-63598522 Msa0464030:five_prime_UTR 20.0%
!!! AATATTTTTACCCATGTTAT+GGG - chr3_3:63598504-63598523 Msa0464030:five_prime_UTR 20.0%
! AGCAATAATAGTTACATTTG+TGG - chr3_3:63596784-63596803 Msa0464030:three_prime_UTR 25.0%
! ACATACAACAACAATAGTTT+AGG - chr3_3:63596924-63596943 Msa0464030:three_prime_UTR 25.0%
! ACTAACTCATGTTGTTAATA+AGG - chr3_3:63597094-63597113 Msa0464030:intron 25.0%
! CTAACTCATGTTGTTAATAA+GGG - chr3_3:63597095-63597114 Msa0464030:intron 25.0%
! GATAGTTAATTGGAGTAATT+AGG - chr3_3:63597342-63597361 Msa0464030:intron 25.0%
! CATCTAAAGAATAGAAAAGT+AGG + chr3_3:63597434-63597453 None:intergenic 25.0%
!!! CTACTTTTCTATTCTTTAGA+TGG - chr3_3:63597432-63597451 Msa0464030:intron 25.0%
! GAAAAGCTGAAAATAAAACA+AGG + chr3_3:63597493-63597512 None:intergenic 25.0%
! TTTCTTTGTATTGAGGATAA+GGG + chr3_3:63597549-63597568 None:intergenic 25.0%
!! TTTTCTTTGTATTGAGGATA+AGG + chr3_3:63597550-63597569 None:intergenic 25.0%
! TATCCTCAATACAAAGAAAA+AGG - chr3_3:63597550-63597569 Msa0464030:CDS 25.0%
!! TAACCTTTTTCTTTGTATTG+AGG + chr3_3:63597556-63597575 None:intergenic 25.0%
! AAAGGTTACTGATATTGTTA+TGG - chr3_3:63597568-63597587 Msa0464030:CDS 25.0%
!! CAATTTACAATTTTCAACAG+AGG + chr3_3:63597822-63597841 None:intergenic 25.0%
!!! CAAAGTTTTTCTTGTTACTT+GGG - chr3_3:63598023-63598042 Msa0464030:CDS 25.0%
! AGATGAGAATACAAATATCA+AGG + chr3_3:63598333-63598352 None:intergenic 25.0%
! AATAATCTTACCCATAACAT+GGG + chr3_3:63598517-63598536 None:intergenic 25.0%
! AGCAATAATAGTTACATTTG+TGG - chr3_3:63596784-63596803 Msa0464030:three_prime_UTR 25.0%
! ACATACAACAACAATAGTTT+AGG - chr3_3:63596924-63596943 Msa0464030:three_prime_UTR 25.0%
! ACTAACTCATGTTGTTAATA+AGG - chr3_3:63597094-63597113 Msa0464030:intron 25.0%
! CTAACTCATGTTGTTAATAA+GGG - chr3_3:63597095-63597114 Msa0464030:intron 25.0%
! GATAGTTAATTGGAGTAATT+AGG - chr3_3:63597342-63597361 Msa0464030:intron 25.0%
! CATCTAAAGAATAGAAAAGT+AGG + chr3_3:63597434-63597453 None:intergenic 25.0%
!!! CTACTTTTCTATTCTTTAGA+TGG - chr3_3:63597432-63597451 Msa0464030:intron 25.0%
! GAAAAGCTGAAAATAAAACA+AGG + chr3_3:63597493-63597512 None:intergenic 25.0%
! TTTCTTTGTATTGAGGATAA+GGG + chr3_3:63597549-63597568 None:intergenic 25.0%
!! TTTTCTTTGTATTGAGGATA+AGG + chr3_3:63597550-63597569 None:intergenic 25.0%
! TATCCTCAATACAAAGAAAA+AGG - chr3_3:63597550-63597569 Msa0464030:CDS 25.0%
!! TAACCTTTTTCTTTGTATTG+AGG + chr3_3:63597556-63597575 None:intergenic 25.0%
! AAAGGTTACTGATATTGTTA+TGG - chr3_3:63597568-63597587 Msa0464030:CDS 25.0%
!! CAATTTACAATTTTCAACAG+AGG + chr3_3:63597822-63597841 None:intergenic 25.0%
!!! CAAAGTTTTTCTTGTTACTT+GGG - chr3_3:63598023-63598042 Msa0464030:CDS 25.0%
! AGATGAGAATACAAATATCA+AGG + chr3_3:63598333-63598352 None:intergenic 25.0%
! AATAATCTTACCCATAACAT+GGG + chr3_3:63598517-63598536 None:intergenic 25.0%
CATTTGTGGTTGCATAAATA+AGG - chr3_3:63596798-63596817 Msa0464030:three_prime_UTR 30.0%
CTTGGAAATCAACATCAAAT+AGG - chr3_3:63596886-63596905 Msa0464030:three_prime_UTR 30.0%
GGGTTTAACATTAACATGTA+TGG - chr3_3:63596981-63597000 Msa0464030:CDS 30.0%
ATTCTAAGCATGCAATGATT+TGG - chr3_3:63597069-63597088 Msa0464030:CDS 30.0%
TTCTAAGCATGCAATGATTT+GGG - chr3_3:63597070-63597089 Msa0464030:CDS 30.0%
ACTTACCTTGTTAATCATCT+TGG - chr3_3:63597179-63597198 Msa0464030:intron 30.0%
CTTACCTTGTTAATCATCTT+GGG - chr3_3:63597180-63597199 Msa0464030:intron 30.0%
TTCTTTGTATTGAGGATAAG+GGG + chr3_3:63597548-63597567 None:intergenic 30.0%
TGTTGTAAATTGCTTGTGTT+TGG + chr3_3:63597742-63597761 None:intergenic 30.0%
! TTTGGAAGTATGACATCAAA+AGG + chr3_3:63598007-63598026 None:intergenic 30.0%
CCAAGTAACAAGAAAAACTT+TGG + chr3_3:63598025-63598044 None:intergenic 30.0%
! CCAAAGTTTTTCTTGTTACT+TGG - chr3_3:63598022-63598041 Msa0464030:CDS 30.0%
!!! TTTTAAGGCACTATGGTAAT+TGG - chr3_3:63598434-63598453 Msa0464030:five_prime_UTR 30.0%
! GTGATTTTCAAACATGTAGT+TGG + chr3_3:63598462-63598481 None:intergenic 30.0%
GAATAATCTTACCCATAACA+TGG + chr3_3:63598518-63598537 None:intergenic 30.0%
TTATGGGTAAGATTATTCTC+TGG - chr3_3:63598520-63598539 Msa0464030:five_prime_UTR 30.0%
TATGGGTAAGATTATTCTCT+GGG - chr3_3:63598521-63598540 Msa0464030:five_prime_UTR 30.0%
CATTTGTGGTTGCATAAATA+AGG - chr3_3:63596798-63596817 Msa0464030:three_prime_UTR 30.0%
CTTGGAAATCAACATCAAAT+AGG - chr3_3:63596886-63596905 Msa0464030:three_prime_UTR 30.0%
GGGTTTAACATTAACATGTA+TGG - chr3_3:63596981-63597000 Msa0464030:CDS 30.0%
ATTCTAAGCATGCAATGATT+TGG - chr3_3:63597069-63597088 Msa0464030:CDS 30.0%
TTCTAAGCATGCAATGATTT+GGG - chr3_3:63597070-63597089 Msa0464030:CDS 30.0%
ACTTACCTTGTTAATCATCT+TGG - chr3_3:63597179-63597198 Msa0464030:intron 30.0%
CTTACCTTGTTAATCATCTT+GGG - chr3_3:63597180-63597199 Msa0464030:intron 30.0%
TTCTTTGTATTGAGGATAAG+GGG + chr3_3:63597548-63597567 None:intergenic 30.0%
TGTTGTAAATTGCTTGTGTT+TGG + chr3_3:63597742-63597761 None:intergenic 30.0%
! TTTGGAAGTATGACATCAAA+AGG + chr3_3:63598007-63598026 None:intergenic 30.0%
CCAAGTAACAAGAAAAACTT+TGG + chr3_3:63598025-63598044 None:intergenic 30.0%
! CCAAAGTTTTTCTTGTTACT+TGG - chr3_3:63598022-63598041 Msa0464030:CDS 30.0%
!!! TTTTAAGGCACTATGGTAAT+TGG - chr3_3:63598434-63598453 Msa0464030:five_prime_UTR 30.0%
! GTGATTTTCAAACATGTAGT+TGG + chr3_3:63598462-63598481 None:intergenic 30.0%
GAATAATCTTACCCATAACA+TGG + chr3_3:63598518-63598537 None:intergenic 30.0%
TTATGGGTAAGATTATTCTC+TGG - chr3_3:63598520-63598539 Msa0464030:five_prime_UTR 30.0%
TATGGGTAAGATTATTCTCT+GGG - chr3_3:63598521-63598540 Msa0464030:five_prime_UTR 30.0%
!!! AGGGGTTTTATTTTTTGTGC+AGG + chr3_3:63596620-63596639 None:intergenic 35.0%
CTAGTTGAATAGCTGAAAGA+GGG + chr3_3:63596766-63596785 None:intergenic 35.0%
CTCAAAAATGAAGCTGAACT+TGG + chr3_3:63596846-63596865 None:intergenic 35.0%
!! CAAGTTCAGCTTCATTTTTG+AGG - chr3_3:63596844-63596863 Msa0464030:three_prime_UTR 35.0%
! TTTGAGGCAGTTAAGTTCAA+AGG - chr3_3:63596860-63596879 Msa0464030:three_prime_UTR 35.0%
AGTTAAGTTCAAAGGAGACT+TGG - chr3_3:63596868-63596887 Msa0464030:three_prime_UTR 35.0%
GGAAATCAACATCAAATAGG+TGG - chr3_3:63596889-63596908 Msa0464030:three_prime_UTR 35.0%
TGAGACAAATCTGAGTCAAA+TGG - chr3_3:63596956-63596975 Msa0464030:CDS 35.0%
ACAAATCTGAGTCAAATGGA+AGG - chr3_3:63596960-63596979 Msa0464030:CDS 35.0%
CAAATCTGAGTCAAATGGAA+GGG - chr3_3:63596961-63596980 Msa0464030:CDS 35.0%
TATGGAAATGAAGAGAGTTG+TGG - chr3_3:63596999-63597018 Msa0464030:CDS 35.0%
TCTTTGTATTGAGGATAAGG+GGG + chr3_3:63597547-63597566 None:intergenic 35.0%
ATATTGTTATGGCCTGTCAT+AGG - chr3_3:63597579-63597598 Msa0464030:CDS 35.0%
TGTAATTCAAGGACCTAAAC+TGG - chr3_3:63597618-63597637 Msa0464030:intron 35.0%
! AGAAAGAAGATGGCTTCTTT+TGG + chr3_3:63597696-63597715 None:intergenic 35.0%
TACCAGCTAAAGAAAGAAGA+TGG + chr3_3:63597706-63597725 None:intergenic 35.0%
TCCATGATATGTCAGTTAAG+TGG - chr3_3:63597763-63597782 Msa0464030:intron 35.0%
TAGAGCTTGAACTTGTAAAC+AGG - chr3_3:63597788-63597807 Msa0464030:intron 35.0%
AGAGCTTGAACTTGTAAACA+GGG - chr3_3:63597789-63597808 Msa0464030:intron 35.0%
TACACTTAGCTGACATATCA+TGG + chr3_3:63597864-63597883 None:intergenic 35.0%
!! TACTAGTCTTAGATCTCAAG+AGG - chr3_3:63598285-63598304 Msa0464030:CDS 35.0%
!! ACTAGTCTTAGATCTCAAGA+GGG - chr3_3:63598286-63598305 Msa0464030:CDS 35.0%
TAGATCTCAAGAGGGAAAAA+TGG - chr3_3:63598294-63598313 Msa0464030:CDS 35.0%
AGATCTCAAGAGGGAAAAAT+GGG - chr3_3:63598295-63598314 Msa0464030:CDS 35.0%
!!! CTTGTCATTTTAAGGCACTA+TGG - chr3_3:63598427-63598446 Msa0464030:five_prime_UTR 35.0%
ATGGGTAAGATTATTCTCTG+GGG - chr3_3:63598522-63598541 Msa0464030:five_prime_UTR 35.0%
!!! AGGGGTTTTATTTTTTGTGC+AGG + chr3_3:63596620-63596639 None:intergenic 35.0%
CTAGTTGAATAGCTGAAAGA+GGG + chr3_3:63596766-63596785 None:intergenic 35.0%
CTCAAAAATGAAGCTGAACT+TGG + chr3_3:63596846-63596865 None:intergenic 35.0%
!! CAAGTTCAGCTTCATTTTTG+AGG - chr3_3:63596844-63596863 Msa0464030:three_prime_UTR 35.0%
! TTTGAGGCAGTTAAGTTCAA+AGG - chr3_3:63596860-63596879 Msa0464030:three_prime_UTR 35.0%
AGTTAAGTTCAAAGGAGACT+TGG - chr3_3:63596868-63596887 Msa0464030:three_prime_UTR 35.0%
GGAAATCAACATCAAATAGG+TGG - chr3_3:63596889-63596908 Msa0464030:three_prime_UTR 35.0%
TGAGACAAATCTGAGTCAAA+TGG - chr3_3:63596956-63596975 Msa0464030:CDS 35.0%
ACAAATCTGAGTCAAATGGA+AGG - chr3_3:63596960-63596979 Msa0464030:CDS 35.0%
CAAATCTGAGTCAAATGGAA+GGG - chr3_3:63596961-63596980 Msa0464030:CDS 35.0%
TATGGAAATGAAGAGAGTTG+TGG - chr3_3:63596999-63597018 Msa0464030:CDS 35.0%
TCTTTGTATTGAGGATAAGG+GGG + chr3_3:63597547-63597566 None:intergenic 35.0%
ATATTGTTATGGCCTGTCAT+AGG - chr3_3:63597579-63597598 Msa0464030:CDS 35.0%
TGTAATTCAAGGACCTAAAC+TGG - chr3_3:63597618-63597637 Msa0464030:intron 35.0%
! AGAAAGAAGATGGCTTCTTT+TGG + chr3_3:63597696-63597715 None:intergenic 35.0%
TACCAGCTAAAGAAAGAAGA+TGG + chr3_3:63597706-63597725 None:intergenic 35.0%
TCCATGATATGTCAGTTAAG+TGG - chr3_3:63597763-63597782 Msa0464030:intron 35.0%
TAGAGCTTGAACTTGTAAAC+AGG - chr3_3:63597788-63597807 Msa0464030:intron 35.0%
AGAGCTTGAACTTGTAAACA+GGG - chr3_3:63597789-63597808 Msa0464030:intron 35.0%
TACACTTAGCTGACATATCA+TGG + chr3_3:63597864-63597883 None:intergenic 35.0%
!! TACTAGTCTTAGATCTCAAG+AGG - chr3_3:63598285-63598304 Msa0464030:CDS 35.0%
!! ACTAGTCTTAGATCTCAAGA+GGG - chr3_3:63598286-63598305 Msa0464030:CDS 35.0%
TAGATCTCAAGAGGGAAAAA+TGG - chr3_3:63598294-63598313 Msa0464030:CDS 35.0%
AGATCTCAAGAGGGAAAAAT+GGG - chr3_3:63598295-63598314 Msa0464030:CDS 35.0%
!!! CTTGTCATTTTAAGGCACTA+TGG - chr3_3:63598427-63598446 Msa0464030:five_prime_UTR 35.0%
ATGGGTAAGATTATTCTCTG+GGG - chr3_3:63598522-63598541 Msa0464030:five_prime_UTR 35.0%
! TTGTGCAGGTTAAAGTGTCA+CGG + chr3_3:63596606-63596625 None:intergenic 40.0%
AGGGGGAAACGTAATAAGTA+GGG + chr3_3:63596639-63596658 None:intergenic 40.0%
GCTAGTTGAATAGCTGAAAG+AGG + chr3_3:63596767-63596786 None:intergenic 40.0%
GGTTGCATAAATAAGGCACA+AGG - chr3_3:63596805-63596824 Msa0464030:three_prime_UTR 40.0%
TCAACATCAAATAGGTGGAG+TGG - chr3_3:63596894-63596913 Msa0464030:three_prime_UTR 40.0%
TGAAGAGAGTTGTGGAATGA+TGG - chr3_3:63597007-63597026 Msa0464030:CDS 40.0%
GATGGTGAGAAAGAGAGTTA+TGG - chr3_3:63597025-63597044 Msa0464030:CDS 40.0%
GAGAAAGAGAGTTATGGTTG+TGG - chr3_3:63597031-63597050 Msa0464030:CDS 40.0%
AGAGTTATGGTTGTGGTTGA+TGG - chr3_3:63597038-63597057 Msa0464030:CDS 40.0%
!! TAAGACTCAGAAGCACTTTG+AGG + chr3_3:63597152-63597171 None:intergenic 40.0%
! TGGCTTGCAATCTTTGCAAA+GGG + chr3_3:63597207-63597226 None:intergenic 40.0%
AAACCAAACCAAGTTACCTC+TGG + chr3_3:63597227-63597246 None:intergenic 40.0%
!! GTGGAAGCACTTGTAATTCA+AGG - chr3_3:63597607-63597626 Msa0464030:intron 40.0%
GATGAGCCAGGTAAAGAAAT+TGG - chr3_3:63597648-63597667 Msa0464030:intron 40.0%
AGACCTCCAATTTCTTTACC+TGG + chr3_3:63597657-63597676 None:intergenic 40.0%
AGCCATCTTCTTTCTTTAGC+TGG - chr3_3:63597701-63597720 Msa0464030:intron 40.0%
GCCACTTAACTGACATATCA+TGG + chr3_3:63597767-63597786 None:intergenic 40.0%
AAACAAGACAATTGGGGTGA+TGG + chr3_3:63597970-63597989 None:intergenic 40.0%
CTGTTTGTTTACAGTCTCTG+TGG - chr3_3:63598089-63598108 Msa0464030:CDS 40.0%
AATGCTGAGTGCTTGACAAT+AGG - chr3_3:63598164-63598183 Msa0464030:CDS 40.0%
AAGAGAAGCCAAGGCAATAA+TGG - chr3_3:63598194-63598213 Msa0464030:CDS 40.0%
TAAGGTAGCCATTATTGCCT+TGG + chr3_3:63598205-63598224 None:intergenic 40.0%
GATCTCAAGAGGGAAAAATG+GGG - chr3_3:63598296-63598315 Msa0464030:CDS 40.0%
!! ATTCACTAGCATTGGCAAGT+TGG + chr3_3:63598484-63598503 None:intergenic 40.0%
! TTGTGCAGGTTAAAGTGTCA+CGG + chr3_3:63596606-63596625 None:intergenic 40.0%
AGGGGGAAACGTAATAAGTA+GGG + chr3_3:63596639-63596658 None:intergenic 40.0%
GCTAGTTGAATAGCTGAAAG+AGG + chr3_3:63596767-63596786 None:intergenic 40.0%
GGTTGCATAAATAAGGCACA+AGG - chr3_3:63596805-63596824 Msa0464030:three_prime_UTR 40.0%
TCAACATCAAATAGGTGGAG+TGG - chr3_3:63596894-63596913 Msa0464030:three_prime_UTR 40.0%
TGAAGAGAGTTGTGGAATGA+TGG - chr3_3:63597007-63597026 Msa0464030:CDS 40.0%
GATGGTGAGAAAGAGAGTTA+TGG - chr3_3:63597025-63597044 Msa0464030:CDS 40.0%
GAGAAAGAGAGTTATGGTTG+TGG - chr3_3:63597031-63597050 Msa0464030:CDS 40.0%
AGAGTTATGGTTGTGGTTGA+TGG - chr3_3:63597038-63597057 Msa0464030:CDS 40.0%
!! TAAGACTCAGAAGCACTTTG+AGG + chr3_3:63597152-63597171 None:intergenic 40.0%
! TGGCTTGCAATCTTTGCAAA+GGG + chr3_3:63597207-63597226 None:intergenic 40.0%
AAACCAAACCAAGTTACCTC+TGG + chr3_3:63597227-63597246 None:intergenic 40.0%
!! GTGGAAGCACTTGTAATTCA+AGG - chr3_3:63597607-63597626 Msa0464030:intron 40.0%
GATGAGCCAGGTAAAGAAAT+TGG - chr3_3:63597648-63597667 Msa0464030:intron 40.0%
AGACCTCCAATTTCTTTACC+TGG + chr3_3:63597657-63597676 None:intergenic 40.0%
AGCCATCTTCTTTCTTTAGC+TGG - chr3_3:63597701-63597720 Msa0464030:intron 40.0%
GCCACTTAACTGACATATCA+TGG + chr3_3:63597767-63597786 None:intergenic 40.0%
AAACAAGACAATTGGGGTGA+TGG + chr3_3:63597970-63597989 None:intergenic 40.0%
CTGTTTGTTTACAGTCTCTG+TGG - chr3_3:63598089-63598108 Msa0464030:CDS 40.0%
AATGCTGAGTGCTTGACAAT+AGG - chr3_3:63598164-63598183 Msa0464030:CDS 40.0%
AAGAGAAGCCAAGGCAATAA+TGG - chr3_3:63598194-63598213 Msa0464030:CDS 40.0%
TAAGGTAGCCATTATTGCCT+TGG + chr3_3:63598205-63598224 None:intergenic 40.0%
GATCTCAAGAGGGAAAAATG+GGG - chr3_3:63598296-63598315 Msa0464030:CDS 40.0%
!! ATTCACTAGCATTGGCAAGT+TGG + chr3_3:63598484-63598503 None:intergenic 40.0%
! TGTGCAGGTTAAAGTGTCAC+GGG + chr3_3:63596605-63596624 None:intergenic 45.0%
GGGGGAAACGTAATAAGTAG+GGG + chr3_3:63596638-63596657 None:intergenic 45.0%
GAGGGGGAAACGTAATAAGT+AGG + chr3_3:63596640-63596659 None:intergenic 45.0%
AGAGAGAGATGACATGTGAG+GGG + chr3_3:63596657-63596676 None:intergenic 45.0%
GAGAGAGAGATGACATGTGA+GGG + chr3_3:63596658-63596677 None:intergenic 45.0%
AGAGAGAGAGATGACATGTG+AGG + chr3_3:63596659-63596678 None:intergenic 45.0%
AGTGTAGCAGAGGTTCAGTT+TGG + chr3_3:63596728-63596747 None:intergenic 45.0%
! TAGCTGAAAGAGGGAGTGTA+GGG + chr3_3:63596757-63596776 None:intergenic 45.0%
! ATAGCTGAAAGAGGGAGTGT+AGG + chr3_3:63596758-63596777 None:intergenic 45.0%
GGGACCCAAGATGATTAACA+AGG + chr3_3:63597187-63597206 None:intergenic 45.0%
! CTGGCTTGCAATCTTTGCAA+AGG + chr3_3:63597208-63597227 None:intergenic 45.0%
TTGCAAAGATTGCAAGCCAG+AGG - chr3_3:63597208-63597227 Msa0464030:intron 45.0%
ATTGCAAGCCAGAGGTAACT+TGG - chr3_3:63597216-63597235 Msa0464030:intron 45.0%
AAGCCAGAGGTAACTTGGTT+TGG - chr3_3:63597221-63597240 Msa0464030:intron 45.0%
TTGTTATGGCCTGTCATAGG+TGG - chr3_3:63597582-63597601 Msa0464030:CDS 45.0%
GAGCCAGGTAAAGAAATTGG+AGG - chr3_3:63597651-63597670 Msa0464030:intron 45.0%
GAAATTGGAGGTCTCTATCC+TGG - chr3_3:63597663-63597682 Msa0464030:intron 45.0%
AGTGCTTGACAATAGGAGTG+AGG - chr3_3:63598171-63598190 Msa0464030:CDS 45.0%
!!! GCAGAAGAACTTCTGGCTTT+TGG - chr3_3:63598238-63598257 Msa0464030:CDS 45.0%
! TGTGCAGGTTAAAGTGTCAC+GGG + chr3_3:63596605-63596624 None:intergenic 45.0%
GGGGGAAACGTAATAAGTAG+GGG + chr3_3:63596638-63596657 None:intergenic 45.0%
GAGGGGGAAACGTAATAAGT+AGG + chr3_3:63596640-63596659 None:intergenic 45.0%
AGAGAGAGATGACATGTGAG+GGG + chr3_3:63596657-63596676 None:intergenic 45.0%
GAGAGAGAGATGACATGTGA+GGG + chr3_3:63596658-63596677 None:intergenic 45.0%
AGAGAGAGAGATGACATGTG+AGG + chr3_3:63596659-63596678 None:intergenic 45.0%
AGTGTAGCAGAGGTTCAGTT+TGG + chr3_3:63596728-63596747 None:intergenic 45.0%
! TAGCTGAAAGAGGGAGTGTA+GGG + chr3_3:63596757-63596776 None:intergenic 45.0%
! ATAGCTGAAAGAGGGAGTGT+AGG + chr3_3:63596758-63596777 None:intergenic 45.0%
GGGACCCAAGATGATTAACA+AGG + chr3_3:63597187-63597206 None:intergenic 45.0%
! CTGGCTTGCAATCTTTGCAA+AGG + chr3_3:63597208-63597227 None:intergenic 45.0%
TTGCAAAGATTGCAAGCCAG+AGG - chr3_3:63597208-63597227 Msa0464030:intron 45.0%
ATTGCAAGCCAGAGGTAACT+TGG - chr3_3:63597216-63597235 Msa0464030:intron 45.0%
AAGCCAGAGGTAACTTGGTT+TGG - chr3_3:63597221-63597240 Msa0464030:intron 45.0%
TTGTTATGGCCTGTCATAGG+TGG - chr3_3:63597582-63597601 Msa0464030:CDS 45.0%
GAGCCAGGTAAAGAAATTGG+AGG - chr3_3:63597651-63597670 Msa0464030:intron 45.0%
GAAATTGGAGGTCTCTATCC+TGG - chr3_3:63597663-63597682 Msa0464030:intron 45.0%
AGTGCTTGACAATAGGAGTG+AGG - chr3_3:63598171-63598190 Msa0464030:CDS 45.0%
!!! GCAGAAGAACTTCTGGCTTT+TGG - chr3_3:63598238-63598257 Msa0464030:CDS 45.0%
GAGAGAGATGACATGTGAGG+GGG + chr3_3:63596656-63596675 None:intergenic 50.0%
CTTCCACTTCCACCTATGAC+AGG + chr3_3:63597594-63597613 None:intergenic 50.0%
CTCATCACTGTGGCCAGTTT+AGG + chr3_3:63597634-63597653 None:intergenic 50.0%
CTTTACCTGGCTCATCACTG+TGG + chr3_3:63597644-63597663 None:intergenic 50.0%
GAGGTCTCTATCCTGGTACT+TGG - chr3_3:63597670-63597689 Msa0464030:intron 50.0%
! GCTTCTTTTGGCCAAGTACC+AGG + chr3_3:63597684-63597703 None:intergenic 50.0%
ATGGCTACCTTATCAGCACC+AGG - chr3_3:63598213-63598232 Msa0464030:CDS 50.0%
GAGAGAGATGACATGTGAGG+GGG + chr3_3:63596656-63596675 None:intergenic 50.0%
CTTCCACTTCCACCTATGAC+AGG + chr3_3:63597594-63597613 None:intergenic 50.0%
CTCATCACTGTGGCCAGTTT+AGG + chr3_3:63597634-63597653 None:intergenic 50.0%
CTTTACCTGGCTCATCACTG+TGG + chr3_3:63597644-63597663 None:intergenic 50.0%
GAGGTCTCTATCCTGGTACT+TGG - chr3_3:63597670-63597689 Msa0464030:intron 50.0%
! GCTTCTTTTGGCCAAGTACC+AGG + chr3_3:63597684-63597703 None:intergenic 50.0%
ATGGCTACCTTATCAGCACC+AGG - chr3_3:63598213-63598232 Msa0464030:CDS 50.0%
AGGGAGCTAGAGTGTAGCAG+AGG + chr3_3:63596738-63596757 None:intergenic 55.0%
TGGCCTGTCATAGGTGGAAG+TGG - chr3_3:63597588-63597607 Msa0464030:CDS 55.0%
CAGCAGCACAGAGGAGTTTG+TGG - chr3_3:63598127-63598146 Msa0464030:CDS 55.0%
GGAGTGAGGAAGAGAAGCCA+AGG - chr3_3:63598185-63598204 Msa0464030:CDS 55.0%
GCTACCTTATCAGCACCAGG+TGG - chr3_3:63598216-63598235 Msa0464030:CDS 55.0%
!! TCTGCCACCTGGTGCTGATA+AGG + chr3_3:63598223-63598242 None:intergenic 55.0%
CCAGAAGTTCTTCTGCCACC+TGG + chr3_3:63598234-63598253 None:intergenic 55.0%
CCAGGTGGCAGAAGAACTTC+TGG - chr3_3:63598231-63598250 Msa0464030:CDS 55.0%
AGGGAGCTAGAGTGTAGCAG+AGG + chr3_3:63596738-63596757 None:intergenic 55.0%
TGGCCTGTCATAGGTGGAAG+TGG - chr3_3:63597588-63597607 Msa0464030:CDS 55.0%
CAGCAGCACAGAGGAGTTTG+TGG - chr3_3:63598127-63598146 Msa0464030:CDS 55.0%
GGAGTGAGGAAGAGAAGCCA+AGG - chr3_3:63598185-63598204 Msa0464030:CDS 55.0%
GCTACCTTATCAGCACCAGG+TGG - chr3_3:63598216-63598235 Msa0464030:CDS 55.0%
!! TCTGCCACCTGGTGCTGATA+AGG + chr3_3:63598223-63598242 None:intergenic 55.0%
CCAGAAGTTCTTCTGCCACC+TGG + chr3_3:63598234-63598253 None:intergenic 55.0%
CCAGGTGGCAGAAGAACTTC+TGG - chr3_3:63598231-63598250 Msa0464030:CDS 55.0%
ACTGGCCACAGTGATGAGCC+AGG - chr3_3:63597636-63597655 Msa0464030:intron 60.0%
ACTGGCCACAGTGATGAGCC+AGG - chr3_3:63597636-63597655 Msa0464030:intron 60.0%
CAGCAGCTGCAGCAGCACAG+AGG - chr3_3:63598118-63598137 Msa0464030:CDS 65.0%
CAGCAGCTGCAGCAGCACAG+AGG - chr3_3:63598118-63598137 Msa0464030:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 63596596 63598616 63596596 ID=Msa0464030;Name=Msa0464030
chr3_3 mRNA 63596596 63598616 63596596 ID=Msa0464030-mRNA-1;Parent=Msa0464030;Name=Msa0464030-mRNA-1;_AED=0.05;_eAED=0.05;_QI=244|1|1|1|1|1|3|351|224
chr3_3 exon 63596596 63597109 63596596 ID=Msa0464030-mRNA-1:exon:13939;Parent=Msa0464030-mRNA-1
chr3_3 exon 63597490 63597611 63597490 ID=Msa0464030-mRNA-1:exon:13938;Parent=Msa0464030-mRNA-1
chr3_3 exon 63597983 63598616 63597983 ID=Msa0464030-mRNA-1:exon:13937;Parent=Msa0464030-mRNA-1
chr3_3 five_prime_UTR 63598373 63598616 63598373 ID=Msa0464030-mRNA-1:five_prime_utr;Parent=Msa0464030-mRNA-1
chr3_3 CDS 63597983 63598372 63597983 ID=Msa0464030-mRNA-1:cds;Parent=Msa0464030-mRNA-1
chr3_3 CDS 63597490 63597611 63597490 ID=Msa0464030-mRNA-1:cds;Parent=Msa0464030-mRNA-1
chr3_3 CDS 63596947 63597109 63596947 ID=Msa0464030-mRNA-1:cds;Parent=Msa0464030-mRNA-1
chr3_3 three_prime_UTR 63596596 63596946 63596596 ID=Msa0464030-mRNA-1:three_prime_utr;Parent=Msa0464030-mRNA-1
Gene Sequence

>Msa0464030

ATGCCAAGTTCAGCTTCATTTTTGAGGCAGTTAAGTTCAAAGGAGACTTGGAAATCAACATCAAATAGGTGGAGTGGAAAAAACACATACAACAACAATAGTTTAGGAGTTACTTGTGAGACAAATCTGAGTCAAATGGAAGGGTTTAACATTAACATGTATGGAAATGAAGAGAGTTGTGGAATGATGGTGAGAAAGAGAGTTATGGTTGTGGTTGATGGTACTTCACATTCTAAGCATGCAATGATTTGGGCACTAACTCATGTTGTTAATAAGGGTGATTTGTTGACTTTGCTTTACATTGTTTCTCCTCAAAGTGCTTCTGAGTCTTATTCTTCTACTTACCTTGTTAATCATCTTGGGTCCCTTTGCAAAGATTGCAAGCCAGAGGTGGAAGTGGAAGCACTTGTAATTCAAGGACCTAAACTGGCCACAGTGATGAGCCAGGTAAAGAAATTGGAGGTCTCTATCCTGGTACTTGGCCAAAAGAAGCCATCTTCTTTCTTTAGCTGTCTCTGTGGAAGTAGCAGCAGCTGCAGCAGCACAGAGGAGTTTGTGGAGTACTGTATCAACAATGCTGAGTGCTTGACAATAGGAGTGAGGAAGAGAAGCCAAGGCAATAATGGCTACCTTATCAGCACCAGGTGGCAGAAGAACTTCTGGCTTTTGGCTTAG
Protein sequence

>Msa0464030

MPSSASFLRQLSSKETWKSTSNRWSGKNTYNNNSLGVTCETNLSQMEGFNINMYGNEESCGMMVRKRVMVVVDGTSHSKHAMIWALTHVVNKGDLLTLLYIVSPQSASESYSSTYLVNHLGSLCKDCKPEVEVEALVIQGPKLATVMSQVKKLEVSILVLGQKKPSSFFSCLCGSSSSCSSTEEFVEYCINNAECLTIGVRKRSQGNNGYLISTRWQKNFWLLA*