AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0369810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0369810 MtrunA17_Chr3g0114601 98.661 224 3 0 1 224 1 224 4.22e-166 456
Msa0369810 MtrunA17_Chr5g0420141 35.795 176 100 4 61 224 62 236 4.44e-30 111
Msa0369810 MtrunA17_Chr4g0061701 34.545 165 100 3 67 224 59 222 1.17e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0369810 AT1G44760.1 58.850 226 73 6 3 224 4 213 8.88e-83 246
Msa0369810 AT1G44760.2 58.850 226 73 6 3 224 75 284 2.07e-81 244
Msa0369810 AT2G03720.2 38.122 181 99 5 56 224 53 232 1.14e-30 113
Msa0369810 AT2G03720.1 39.157 166 90 4 69 224 1 165 1.42e-29 108
Msa0369810 AT1G69080.1 32.222 180 101 3 66 224 44 223 1.96e-28 107
Msa0369810 AT5G17390.1 36.310 168 97 3 67 224 118 285 2.59e-26 103
Msa0369810 AT1G69080.2 34.706 170 96 5 66 224 44 209 5.26e-26 100
Msa0369810 AT3G03290.1 34.503 171 96 4 67 224 107 274 1.51e-23 95.5
Msa0369810 AT4G13450.1 25.728 206 105 4 66 224 15 219 7.86e-16 73.9

Find 82 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTACTCATGTTGTTAATA+AGG 0.194266 3_1:-67131673 Msa0369810:CDS
GCAGAAGAACTTCTGGCTTT+TGG 0.227711 3_1:-67130538 Msa0369810:CDS
CATTTGTGGTTGCATAAATA+AGG 0.255308 3_1:-67131969 Msa0369810:five_prime_UTR
AGATTATTACTTACTATAGA+TGG 0.287966 3_1:+67132066 None:intergenic
ATTCTAAGCATGCAATGATT+TGG 0.290584 3_1:-67131698 Msa0369810:CDS
TAAAGTGTCACGGGAATCTT+TGG 0.317138 3_1:+67132172 None:intergenic
TAGATCTCAAGAGGGGAAAA+TGG 0.321677 3_1:-67130482 Msa0369810:three_prime_UTR
CTCATCACTGTGGCCAGTTT+AGG 0.329869 3_1:+67131145 None:intergenic
ACTTACCTTGTTAATCATCT+TGG 0.331349 3_1:-67131588 Msa0369810:CDS
AGAAAGAAGATGGCTTCTTT+TGG 0.334209 3_1:+67131083 None:intergenic
GATGAGCCAGGTAAAGAAAT+TGG 0.339137 3_1:-67131128 Msa0369810:CDS
CCAGGTGGCAGAAGAACTTC+TGG 0.346204 3_1:-67130545 Msa0369810:CDS
CTTACCTTGTTAATCATCTT+GGG 0.346303 3_1:-67131587 Msa0369810:CDS
TTCTAAGCATGCAATGATTT+GGG 0.371229 3_1:-67131697 Msa0369810:CDS
CTTACTCATGTTGTTAATAA+GGG 0.375126 3_1:-67131672 Msa0369810:CDS
GGGTTTAACATTAACATGTA+TGG 0.388232 3_1:-67131786 Msa0369810:CDS
CTTGGAAATCAACATCAAAT+AGG 0.391956 3_1:-67131881 Msa0369810:CDS
AAGAGAAGCCAAGGCAATAA+TGG 0.394438 3_1:-67130582 Msa0369810:CDS
ACATACAACAACAATAGTTT+AGG 0.394691 3_1:-67131843 Msa0369810:CDS
AGCCATCTTCTTTCTTTAGC+TGG 0.402605 3_1:-67131075 Msa0369810:intron
AGATCTCAAGAGGGGAAAAT+GGG 0.404854 3_1:-67130481 Msa0369810:three_prime_UTR
AGCAATAATAGTTACATTTG+TGG 0.408337 3_1:-67131983 Msa0369810:five_prime_UTR
AGAGTTATGGTTGTGGTTGA+TGG 0.409544 3_1:-67131729 Msa0369810:CDS
TGTAATTCAAGGACCTAAAC+TGG 0.413139 3_1:-67131158 Msa0369810:CDS
CAGCAGCACAGAGGAGTTTG+TGG 0.433556 3_1:-67130649 Msa0369810:CDS
TCTGCCACCTGGTGCTGATA+AGG 0.435349 3_1:+67130556 None:intergenic
AATGCTGAGTGCTTGACAAT+AGG 0.444116 3_1:-67130612 Msa0369810:CDS
TAGCTGAAAGAGGGAGTGTA+GGG 0.448424 3_1:+67132013 None:intergenic
TGAGACAAATCTGAGTCAAA+TGG 0.450642 3_1:-67131811 Msa0369810:CDS
TAAGGTAGCCATTATTGCCT+TGG 0.456289 3_1:+67130574 None:intergenic
TAAGACTCAGAAGCACTTTG+AGG 0.458252 3_1:+67131618 None:intergenic
AAAATATTATTCACTAGCAT+TGG 0.464290 3_1:+67130287 None:intergenic
AGTGTAGCAGAGGTTCAGTT+TGG 0.464785 3_1:+67132042 None:intergenic
ATAGCTGAAAGAGGGAGTGT+AGG 0.471533 3_1:+67132012 None:intergenic
GAGAGAGAGATGACATGTGA+GGG 0.485365 3_1:+67132110 None:intergenic
TGGCTTGCAATCTTTGCAAA+GGG 0.487254 3_1:+67131563 None:intergenic
ATTCACTAGCATTGGCAAGT+TGG 0.496266 3_1:+67130295 None:intergenic
GAAATTGGAGGTCTCTATCC+TGG 0.499739 3_1:-67131113 Msa0369810:CDS
TATGGGTAAAGATTATTCTC+TGG 0.503481 3_1:-67130255 Msa0369810:three_prime_UTR
GTGGAAGCACTTGTAATTCA+AGG 0.506797 3_1:-67131169 Msa0369810:CDS
AGACCTCCAATTTCTTTACC+TGG 0.507576 3_1:+67131122 None:intergenic
CCAGAAGTTCTTCTGCCACC+TGG 0.508759 3_1:+67130545 None:intergenic
TGGCCTGTCATAGGTGGAAG+TGG 0.514126 3_1:-67131188 Msa0369810:intron
TGTGCAGATTAAAGTGTCAC+GGG 0.525016 3_1:+67132163 None:intergenic
TTTGAGGCAGTTAAGTTCAA+AGG 0.525241 3_1:-67131907 Msa0369810:CDS
CTGGCTTGCAATCTTTGCAA+AGG 0.528141 3_1:+67131562 None:intergenic
TGAAGAGAGTTGTGGAATGA+TGG 0.529080 3_1:-67131760 Msa0369810:CDS
GATTATTACTTACTATAGAT+GGG 0.534676 3_1:+67132067 None:intergenic
GAGGTCTCTATCCTGGTACT+TGG 0.534821 3_1:-67131106 Msa0369810:CDS
TACCAGCTAAAGAAAGAAGA+TGG 0.539360 3_1:+67131073 None:intergenic
AGTTAAGTTCAAAGGAGACT+TGG 0.544950 3_1:-67131899 Msa0369810:CDS
TTGTGCAGATTAAAGTGTCA+CGG 0.548162 3_1:+67132162 None:intergenic
GCTAGTTGAATAGCTGAAAG+AGG 0.552730 3_1:+67132003 None:intergenic
AGATGAGAATACAAATATCA+AGG 0.553739 3_1:+67130446 None:intergenic
GATGGTGAGAAAGAGAGTTA+TGG 0.559684 3_1:-67131742 Msa0369810:CDS
AGAGAGAGAGATGACATGTG+AGG 0.561615 3_1:+67132109 None:intergenic
ACTGGCCACAGTGATGAGCC+AGG 0.573013 3_1:-67131140 Msa0369810:CDS
TCAACATCAAATAGGTGGAG+TGG 0.576570 3_1:-67131873 Msa0369810:CDS
GAGAGAGATGACATGTGAGG+GGG 0.576854 3_1:+67132112 None:intergenic
AGAGAGAGATGACATGTGAG+GGG 0.582282 3_1:+67132111 None:intergenic
ACAAATCTGAGTCAAATGGA+AGG 0.584700 3_1:-67131807 Msa0369810:CDS
TACTAGTCTTAGATCTCAAG+AGG 0.588812 3_1:-67130491 Msa0369810:three_prime_UTR
CTGTTTGTTTACAGTCTCTG+TGG 0.592127 3_1:-67130687 Msa0369810:intron
GGAGTGAGGAAGAGAAGCCA+AGG 0.601340 3_1:-67130591 Msa0369810:CDS
CTAGTTGAATAGCTGAAAGA+GGG 0.601553 3_1:+67132004 None:intergenic
CAAATCTGAGTCAAATGGAA+GGG 0.609381 3_1:-67131806 Msa0369810:CDS
GGAAATCAACATCAAATAGG+TGG 0.613048 3_1:-67131878 Msa0369810:CDS
ACTAGTCTTAGATCTCAAGA+GGG 0.615258 3_1:-67130490 Msa0369810:three_prime_UTR
CAGCAGCTGCAGCAGCACAG+AGG 0.618197 3_1:-67130658 Msa0369810:CDS
ATGGCTACCTTATCAGCACC+AGG 0.619844 3_1:-67130563 Msa0369810:CDS
TATGGAAATGAAGAGAGTTG+TGG 0.622956 3_1:-67131768 Msa0369810:CDS
GAGCCAGGTAAAGAAATTGG+AGG 0.635543 3_1:-67131125 Msa0369810:CDS
GATCTCAAGAGGGGAAAATG+GGG 0.639595 3_1:-67130480 Msa0369810:three_prime_UTR
TTGCAAAGATTGCAAGCCAG+AGG 0.640746 3_1:-67131559 Msa0369810:intron
GAGAAAGAGAGTTATGGTTG+TGG 0.642404 3_1:-67131736 Msa0369810:CDS
AGGGAGCTAGAGTGTAGCAG+AGG 0.656163 3_1:+67132032 None:intergenic
CTTTACCTGGCTCATCACTG+TGG 0.657425 3_1:+67131135 None:intergenic
GGGACCCAAGATGATTAACA+AGG 0.679967 3_1:+67131583 None:intergenic
AGTGCTTGACAATAGGAGTG+AGG 0.688153 3_1:-67130605 Msa0369810:CDS
GGTTGCATAAATAAGGCACA+AGG 0.702121 3_1:-67131962 Msa0369810:five_prime_UTR
CTAGTCTTAGATCTCAAGAG+GGG 0.706285 3_1:-67130489 Msa0369810:three_prime_UTR
GCTACCTTATCAGCACCAGG+TGG 0.765926 3_1:-67130560 Msa0369810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGTAGGAAAATAATTTA+TGG + chr3_1:67130994-67131013 None:intergenic 15.0%
!!! TTTTTTAAAACAAGACAATT+GGG + chr3_1:67131551-67131570 None:intergenic 15.0%
!!! ATTTTTTAAAACAAGACAAT+TGG + chr3_1:67131552-67131571 None:intergenic 15.0%
!!! TAATATTTTTACTCATGTTA+TGG - chr3_1:67132077-67132096 Msa0369810:five_prime_UTR 15.0%
!!! AATATTTTTACTCATGTTAT+GGG - chr3_1:67132078-67132097 Msa0369810:five_prime_UTR 15.0%
!! AAAAGTAGGAAAATAATTTA+TGG + chr3_1:67130994-67131013 None:intergenic 15.0%
!!! TTTTTTAAAACAAGACAATT+GGG + chr3_1:67131551-67131570 None:intergenic 15.0%
!!! ATTTTTTAAAACAAGACAAT+TGG + chr3_1:67131552-67131571 None:intergenic 15.0%
!!! TAATATTTTTACTCATGTTA+TGG - chr3_1:67132077-67132096 Msa0369810:five_prime_UTR 15.0%
!!! AATATTTTTACTCATGTTAT+GGG - chr3_1:67132078-67132097 Msa0369810:five_prime_UTR 15.0%
!! GATTATTACTTACTATAGAT+GGG + chr3_1:67130286-67130305 None:intergenic 20.0%
!! AGATTATTACTTACTATAGA+TGG + chr3_1:67130287-67130306 None:intergenic 20.0%
!!! TTGTTCAATTGATAGTTAAT+TGG - chr3_1:67130915-67130934 Msa0369810:intron 20.0%
!! ATTAGTTGAAAAGAGAAAAA+GGG + chr3_1:67130961-67130980 None:intergenic 20.0%
!! AATTAGTTGAAAAGAGAAAA+AGG + chr3_1:67130962-67130981 None:intergenic 20.0%
!! AATTTACTATTGTCAATTAG+AGG + chr3_1:67131492-67131511 None:intergenic 20.0%
!!! TTTTTAAAACAAGACAATTG+GGG + chr3_1:67131550-67131569 None:intergenic 20.0%
!!! TAATAAGTCTTGTCATTTTA+AGG - chr3_1:67131993-67132012 Msa0369810:five_prime_UTR 20.0%
!! AAAATATTATTCACTAGCAT+TGG + chr3_1:67132066-67132085 None:intergenic 20.0%
!! GATTATTACTTACTATAGAT+GGG + chr3_1:67130286-67130305 None:intergenic 20.0%
!! AGATTATTACTTACTATAGA+TGG + chr3_1:67130287-67130306 None:intergenic 20.0%
!!! TTGTTCAATTGATAGTTAAT+TGG - chr3_1:67130915-67130934 Msa0369810:intron 20.0%
!! ATTAGTTGAAAAGAGAAAAA+GGG + chr3_1:67130961-67130980 None:intergenic 20.0%
!! AATTAGTTGAAAAGAGAAAA+AGG + chr3_1:67130962-67130981 None:intergenic 20.0%
!! AATTTACTATTGTCAATTAG+AGG + chr3_1:67131492-67131511 None:intergenic 20.0%
!!! TTTTTAAAACAAGACAATTG+GGG + chr3_1:67131550-67131569 None:intergenic 20.0%
!!! TAATAAGTCTTGTCATTTTA+AGG - chr3_1:67131993-67132012 Msa0369810:five_prime_UTR 20.0%
!! AAAATATTATTCACTAGCAT+TGG + chr3_1:67132066-67132085 None:intergenic 20.0%
! AGCAATAATAGTTACATTTG+TGG - chr3_1:67130367-67130386 Msa0369810:three_prime_UTR 25.0%
! ACATACAACAACAATAGTTT+AGG - chr3_1:67130507-67130526 Msa0369810:three_prime_UTR 25.0%
! ACTTACTCATGTTGTTAATA+AGG - chr3_1:67130677-67130696 Msa0369810:intron 25.0%
! CTTACTCATGTTGTTAATAA+GGG - chr3_1:67130678-67130697 Msa0369810:intron 25.0%
! GATAGTTAATTGGAGTAATT+AGG - chr3_1:67130925-67130944 Msa0369810:intron 25.0%
! CATCTAAAGAATAGAAAAGT+AGG + chr3_1:67131008-67131027 None:intergenic 25.0%
!!! CTACTTTTCTATTCTTTAGA+TGG - chr3_1:67131006-67131025 Msa0369810:intron 25.0%
! GAAAAGCTGAAAATAAAACA+AGG + chr3_1:67131067-67131086 None:intergenic 25.0%
! TTTCTTTGTATTGAGGATAA+GGG + chr3_1:67131123-67131142 None:intergenic 25.0%
!! TTTTCTTTGTATTGAGGATA+AGG + chr3_1:67131124-67131143 None:intergenic 25.0%
! TATCCTCAATACAAAGAAAA+AGG - chr3_1:67131124-67131143 Msa0369810:CDS 25.0%
!! TAACCTTTTTCTTTGTATTG+AGG + chr3_1:67131130-67131149 None:intergenic 25.0%
! AAAGGTTACTGATATTGTTA+TGG - chr3_1:67131142-67131161 Msa0369810:CDS 25.0%
!!! CAAAGTTTTTCTTGTTACTT+GGG - chr3_1:67131597-67131616 Msa0369810:CDS 25.0%
! AGATGAGAATACAAATATCA+AGG + chr3_1:67131907-67131926 None:intergenic 25.0%
! AGCAATAATAGTTACATTTG+TGG - chr3_1:67130367-67130386 Msa0369810:three_prime_UTR 25.0%
! ACATACAACAACAATAGTTT+AGG - chr3_1:67130507-67130526 Msa0369810:three_prime_UTR 25.0%
! ACTTACTCATGTTGTTAATA+AGG - chr3_1:67130677-67130696 Msa0369810:intron 25.0%
! CTTACTCATGTTGTTAATAA+GGG - chr3_1:67130678-67130697 Msa0369810:intron 25.0%
! GATAGTTAATTGGAGTAATT+AGG - chr3_1:67130925-67130944 Msa0369810:intron 25.0%
! CATCTAAAGAATAGAAAAGT+AGG + chr3_1:67131008-67131027 None:intergenic 25.0%
!!! CTACTTTTCTATTCTTTAGA+TGG - chr3_1:67131006-67131025 Msa0369810:intron 25.0%
! GAAAAGCTGAAAATAAAACA+AGG + chr3_1:67131067-67131086 None:intergenic 25.0%
! TTTCTTTGTATTGAGGATAA+GGG + chr3_1:67131123-67131142 None:intergenic 25.0%
!! TTTTCTTTGTATTGAGGATA+AGG + chr3_1:67131124-67131143 None:intergenic 25.0%
! TATCCTCAATACAAAGAAAA+AGG - chr3_1:67131124-67131143 Msa0369810:CDS 25.0%
!! TAACCTTTTTCTTTGTATTG+AGG + chr3_1:67131130-67131149 None:intergenic 25.0%
! AAAGGTTACTGATATTGTTA+TGG - chr3_1:67131142-67131161 Msa0369810:CDS 25.0%
!!! CAAAGTTTTTCTTGTTACTT+GGG - chr3_1:67131597-67131616 Msa0369810:CDS 25.0%
! AGATGAGAATACAAATATCA+AGG + chr3_1:67131907-67131926 None:intergenic 25.0%
CATTTGTGGTTGCATAAATA+AGG - chr3_1:67130381-67130400 Msa0369810:three_prime_UTR 30.0%
CTTGGAAATCAACATCAAAT+AGG - chr3_1:67130469-67130488 Msa0369810:three_prime_UTR 30.0%
GGGTTTAACATTAACATGTA+TGG - chr3_1:67130564-67130583 Msa0369810:CDS 30.0%
ATTCTAAGCATGCAATGATT+TGG - chr3_1:67130652-67130671 Msa0369810:CDS 30.0%
TTCTAAGCATGCAATGATTT+GGG - chr3_1:67130653-67130672 Msa0369810:CDS 30.0%
ACTTACCTTGTTAATCATCT+TGG - chr3_1:67130762-67130781 Msa0369810:intron 30.0%
CTTACCTTGTTAATCATCTT+GGG - chr3_1:67130763-67130782 Msa0369810:intron 30.0%
TTCTTTGTATTGAGGATAAG+GGG + chr3_1:67131122-67131141 None:intergenic 30.0%
TGTTGTAAATTGCTTGTGTT+TGG + chr3_1:67131316-67131335 None:intergenic 30.0%
! CAATGTACAATTTTCAACAG+AGG + chr3_1:67131396-67131415 None:intergenic 30.0%
! TTTGGAAGTATGACATCAAA+AGG + chr3_1:67131581-67131600 None:intergenic 30.0%
CCAAGTAACAAGAAAAACTT+TGG + chr3_1:67131599-67131618 None:intergenic 30.0%
! CCAAAGTTTTTCTTGTTACT+TGG - chr3_1:67131596-67131615 Msa0369810:CDS 30.0%
!!! TTTTAAGGCACTATGGTAAT+TGG - chr3_1:67132008-67132027 Msa0369810:five_prime_UTR 30.0%
! GTGATTTTCAAACATGTAGT+TGG + chr3_1:67132036-67132055 None:intergenic 30.0%
TATGGGTAAAGATTATTCTC+TGG - chr3_1:67132095-67132114 Msa0369810:five_prime_UTR 30.0%
CATTTGTGGTTGCATAAATA+AGG - chr3_1:67130381-67130400 Msa0369810:three_prime_UTR 30.0%
CTTGGAAATCAACATCAAAT+AGG - chr3_1:67130469-67130488 Msa0369810:three_prime_UTR 30.0%
GGGTTTAACATTAACATGTA+TGG - chr3_1:67130564-67130583 Msa0369810:CDS 30.0%
ATTCTAAGCATGCAATGATT+TGG - chr3_1:67130652-67130671 Msa0369810:CDS 30.0%
TTCTAAGCATGCAATGATTT+GGG - chr3_1:67130653-67130672 Msa0369810:CDS 30.0%
ACTTACCTTGTTAATCATCT+TGG - chr3_1:67130762-67130781 Msa0369810:intron 30.0%
CTTACCTTGTTAATCATCTT+GGG - chr3_1:67130763-67130782 Msa0369810:intron 30.0%
TTCTTTGTATTGAGGATAAG+GGG + chr3_1:67131122-67131141 None:intergenic 30.0%
TGTTGTAAATTGCTTGTGTT+TGG + chr3_1:67131316-67131335 None:intergenic 30.0%
! CAATGTACAATTTTCAACAG+AGG + chr3_1:67131396-67131415 None:intergenic 30.0%
! TTTGGAAGTATGACATCAAA+AGG + chr3_1:67131581-67131600 None:intergenic 30.0%
CCAAGTAACAAGAAAAACTT+TGG + chr3_1:67131599-67131618 None:intergenic 30.0%
! CCAAAGTTTTTCTTGTTACT+TGG - chr3_1:67131596-67131615 Msa0369810:CDS 30.0%
!!! TTTTAAGGCACTATGGTAAT+TGG - chr3_1:67132008-67132027 Msa0369810:five_prime_UTR 30.0%
! GTGATTTTCAAACATGTAGT+TGG + chr3_1:67132036-67132055 None:intergenic 30.0%
TATGGGTAAAGATTATTCTC+TGG - chr3_1:67132095-67132114 Msa0369810:five_prime_UTR 30.0%
! TTGTGCAGATTAAAGTGTCA+CGG + chr3_1:67130191-67130210 None:intergenic 35.0%
CTAGTTGAATAGCTGAAAGA+GGG + chr3_1:67130349-67130368 None:intergenic 35.0%
CTCAAAAATGAAGCTGAACT+TGG + chr3_1:67130429-67130448 None:intergenic 35.0%
!! CAAGTTCAGCTTCATTTTTG+AGG - chr3_1:67130427-67130446 Msa0369810:three_prime_UTR 35.0%
! TTTGAGGCAGTTAAGTTCAA+AGG - chr3_1:67130443-67130462 Msa0369810:three_prime_UTR 35.0%
AGTTAAGTTCAAAGGAGACT+TGG - chr3_1:67130451-67130470 Msa0369810:three_prime_UTR 35.0%
GGAAATCAACATCAAATAGG+TGG - chr3_1:67130472-67130491 Msa0369810:three_prime_UTR 35.0%
TGAGACAAATCTGAGTCAAA+TGG - chr3_1:67130539-67130558 Msa0369810:CDS 35.0%
ACAAATCTGAGTCAAATGGA+AGG - chr3_1:67130543-67130562 Msa0369810:CDS 35.0%
CAAATCTGAGTCAAATGGAA+GGG - chr3_1:67130544-67130563 Msa0369810:CDS 35.0%
TATGGAAATGAAGAGAGTTG+TGG - chr3_1:67130582-67130601 Msa0369810:CDS 35.0%
TCTTTGTATTGAGGATAAGG+GGG + chr3_1:67131121-67131140 None:intergenic 35.0%
ATATTGTTATGGCCTGTCAT+AGG - chr3_1:67131153-67131172 Msa0369810:CDS 35.0%
TGTAATTCAAGGACCTAAAC+TGG - chr3_1:67131192-67131211 Msa0369810:intron 35.0%
! AGAAAGAAGATGGCTTCTTT+TGG + chr3_1:67131270-67131289 None:intergenic 35.0%
TACCAGCTAAAGAAAGAAGA+TGG + chr3_1:67131280-67131299 None:intergenic 35.0%
TCCATGATATGTCAGTTAAG+TGG - chr3_1:67131337-67131356 Msa0369810:intron 35.0%
TAGAGCTTGAACTTGTAAAC+AGG - chr3_1:67131362-67131381 Msa0369810:intron 35.0%
AGAGCTTGAACTTGTAAACA+GGG - chr3_1:67131363-67131382 Msa0369810:intron 35.0%
TACACTTAGCTGACATATCA+CGG + chr3_1:67131438-67131457 None:intergenic 35.0%
!! TACTAGTCTTAGATCTCAAG+AGG - chr3_1:67131859-67131878 Msa0369810:CDS 35.0%
!! ACTAGTCTTAGATCTCAAGA+GGG - chr3_1:67131860-67131879 Msa0369810:CDS 35.0%
!!! CTTGTCATTTTAAGGCACTA+TGG - chr3_1:67132001-67132020 Msa0369810:five_prime_UTR 35.0%
! TTGTGCAGATTAAAGTGTCA+CGG + chr3_1:67130191-67130210 None:intergenic 35.0%
CTAGTTGAATAGCTGAAAGA+GGG + chr3_1:67130349-67130368 None:intergenic 35.0%
CTCAAAAATGAAGCTGAACT+TGG + chr3_1:67130429-67130448 None:intergenic 35.0%
!! CAAGTTCAGCTTCATTTTTG+AGG - chr3_1:67130427-67130446 Msa0369810:three_prime_UTR 35.0%
! TTTGAGGCAGTTAAGTTCAA+AGG - chr3_1:67130443-67130462 Msa0369810:three_prime_UTR 35.0%
AGTTAAGTTCAAAGGAGACT+TGG - chr3_1:67130451-67130470 Msa0369810:three_prime_UTR 35.0%
GGAAATCAACATCAAATAGG+TGG - chr3_1:67130472-67130491 Msa0369810:three_prime_UTR 35.0%
TGAGACAAATCTGAGTCAAA+TGG - chr3_1:67130539-67130558 Msa0369810:CDS 35.0%
ACAAATCTGAGTCAAATGGA+AGG - chr3_1:67130543-67130562 Msa0369810:CDS 35.0%
CAAATCTGAGTCAAATGGAA+GGG - chr3_1:67130544-67130563 Msa0369810:CDS 35.0%
TATGGAAATGAAGAGAGTTG+TGG - chr3_1:67130582-67130601 Msa0369810:CDS 35.0%
TCTTTGTATTGAGGATAAGG+GGG + chr3_1:67131121-67131140 None:intergenic 35.0%
ATATTGTTATGGCCTGTCAT+AGG - chr3_1:67131153-67131172 Msa0369810:CDS 35.0%
TGTAATTCAAGGACCTAAAC+TGG - chr3_1:67131192-67131211 Msa0369810:intron 35.0%
! AGAAAGAAGATGGCTTCTTT+TGG + chr3_1:67131270-67131289 None:intergenic 35.0%
TACCAGCTAAAGAAAGAAGA+TGG + chr3_1:67131280-67131299 None:intergenic 35.0%
TCCATGATATGTCAGTTAAG+TGG - chr3_1:67131337-67131356 Msa0369810:intron 35.0%
TAGAGCTTGAACTTGTAAAC+AGG - chr3_1:67131362-67131381 Msa0369810:intron 35.0%
AGAGCTTGAACTTGTAAACA+GGG - chr3_1:67131363-67131382 Msa0369810:intron 35.0%
TACACTTAGCTGACATATCA+CGG + chr3_1:67131438-67131457 None:intergenic 35.0%
!! TACTAGTCTTAGATCTCAAG+AGG - chr3_1:67131859-67131878 Msa0369810:CDS 35.0%
!! ACTAGTCTTAGATCTCAAGA+GGG - chr3_1:67131860-67131879 Msa0369810:CDS 35.0%
!!! CTTGTCATTTTAAGGCACTA+TGG - chr3_1:67132001-67132020 Msa0369810:five_prime_UTR 35.0%
! TGTGCAGATTAAAGTGTCAC+GGG + chr3_1:67130190-67130209 None:intergenic 40.0%
AGGGGGAAACGTAATAAGTA+GGG + chr3_1:67130224-67130243 None:intergenic 40.0%
GCTAGTTGAATAGCTGAAAG+AGG + chr3_1:67130350-67130369 None:intergenic 40.0%
GGTTGCATAAATAAGGCACA+AGG - chr3_1:67130388-67130407 Msa0369810:three_prime_UTR 40.0%
TCAACATCAAATAGGTGGAG+TGG - chr3_1:67130477-67130496 Msa0369810:three_prime_UTR 40.0%
TGAAGAGAGTTGTGGAATGA+TGG - chr3_1:67130590-67130609 Msa0369810:CDS 40.0%
GATGGTGAGAAAGAGAGTTA+TGG - chr3_1:67130608-67130627 Msa0369810:CDS 40.0%
GAGAAAGAGAGTTATGGTTG+TGG - chr3_1:67130614-67130633 Msa0369810:CDS 40.0%
AGAGTTATGGTTGTGGTTGA+TGG - chr3_1:67130621-67130640 Msa0369810:CDS 40.0%
!! TAAGACTCAGAAGCACTTTG+AGG + chr3_1:67130735-67130754 None:intergenic 40.0%
! TGGCTTGCAATCTTTGCAAA+GGG + chr3_1:67130790-67130809 None:intergenic 40.0%
AAACCAAACCAAGTTACCTC+TGG + chr3_1:67130810-67130829 None:intergenic 40.0%
!! GTGGAAGCACTTGTAATTCA+AGG - chr3_1:67131181-67131200 Msa0369810:intron 40.0%
GATGAGCCAGGTAAAGAAAT+TGG - chr3_1:67131222-67131241 Msa0369810:intron 40.0%
AGACCTCCAATTTCTTTACC+TGG + chr3_1:67131231-67131250 None:intergenic 40.0%
AGCCATCTTCTTTCTTTAGC+TGG - chr3_1:67131275-67131294 Msa0369810:intron 40.0%
GCCACTTAACTGACATATCA+TGG + chr3_1:67131341-67131360 None:intergenic 40.0%
AAACAAGACAATTGGGGTGA+TGG + chr3_1:67131544-67131563 None:intergenic 40.0%
CTGTTTGTTTACAGTCTCTG+TGG - chr3_1:67131663-67131682 Msa0369810:CDS 40.0%
AATGCTGAGTGCTTGACAAT+AGG - chr3_1:67131738-67131757 Msa0369810:CDS 40.0%
AAGAGAAGCCAAGGCAATAA+TGG - chr3_1:67131768-67131787 Msa0369810:CDS 40.0%
TAAGGTAGCCATTATTGCCT+TGG + chr3_1:67131779-67131798 None:intergenic 40.0%
!! CTAGTCTTAGATCTCAAGAG+GGG - chr3_1:67131861-67131880 Msa0369810:CDS 40.0%
TAGATCTCAAGAGGGGAAAA+TGG - chr3_1:67131868-67131887 Msa0369810:CDS 40.0%
AGATCTCAAGAGGGGAAAAT+GGG - chr3_1:67131869-67131888 Msa0369810:CDS 40.0%
!! ATTCACTAGCATTGGCAAGT+TGG + chr3_1:67132058-67132077 None:intergenic 40.0%
! TGTGCAGATTAAAGTGTCAC+GGG + chr3_1:67130190-67130209 None:intergenic 40.0%
AGGGGGAAACGTAATAAGTA+GGG + chr3_1:67130224-67130243 None:intergenic 40.0%
GCTAGTTGAATAGCTGAAAG+AGG + chr3_1:67130350-67130369 None:intergenic 40.0%
GGTTGCATAAATAAGGCACA+AGG - chr3_1:67130388-67130407 Msa0369810:three_prime_UTR 40.0%
TCAACATCAAATAGGTGGAG+TGG - chr3_1:67130477-67130496 Msa0369810:three_prime_UTR 40.0%
TGAAGAGAGTTGTGGAATGA+TGG - chr3_1:67130590-67130609 Msa0369810:CDS 40.0%
GATGGTGAGAAAGAGAGTTA+TGG - chr3_1:67130608-67130627 Msa0369810:CDS 40.0%
GAGAAAGAGAGTTATGGTTG+TGG - chr3_1:67130614-67130633 Msa0369810:CDS 40.0%
AGAGTTATGGTTGTGGTTGA+TGG - chr3_1:67130621-67130640 Msa0369810:CDS 40.0%
!! TAAGACTCAGAAGCACTTTG+AGG + chr3_1:67130735-67130754 None:intergenic 40.0%
! TGGCTTGCAATCTTTGCAAA+GGG + chr3_1:67130790-67130809 None:intergenic 40.0%
AAACCAAACCAAGTTACCTC+TGG + chr3_1:67130810-67130829 None:intergenic 40.0%
!! GTGGAAGCACTTGTAATTCA+AGG - chr3_1:67131181-67131200 Msa0369810:intron 40.0%
GATGAGCCAGGTAAAGAAAT+TGG - chr3_1:67131222-67131241 Msa0369810:intron 40.0%
AGACCTCCAATTTCTTTACC+TGG + chr3_1:67131231-67131250 None:intergenic 40.0%
AGCCATCTTCTTTCTTTAGC+TGG - chr3_1:67131275-67131294 Msa0369810:intron 40.0%
GCCACTTAACTGACATATCA+TGG + chr3_1:67131341-67131360 None:intergenic 40.0%
AAACAAGACAATTGGGGTGA+TGG + chr3_1:67131544-67131563 None:intergenic 40.0%
CTGTTTGTTTACAGTCTCTG+TGG - chr3_1:67131663-67131682 Msa0369810:CDS 40.0%
AATGCTGAGTGCTTGACAAT+AGG - chr3_1:67131738-67131757 Msa0369810:CDS 40.0%
AAGAGAAGCCAAGGCAATAA+TGG - chr3_1:67131768-67131787 Msa0369810:CDS 40.0%
TAAGGTAGCCATTATTGCCT+TGG + chr3_1:67131779-67131798 None:intergenic 40.0%
!! CTAGTCTTAGATCTCAAGAG+GGG - chr3_1:67131861-67131880 Msa0369810:CDS 40.0%
TAGATCTCAAGAGGGGAAAA+TGG - chr3_1:67131868-67131887 Msa0369810:CDS 40.0%
AGATCTCAAGAGGGGAAAAT+GGG - chr3_1:67131869-67131888 Msa0369810:CDS 40.0%
!! ATTCACTAGCATTGGCAAGT+TGG + chr3_1:67132058-67132077 None:intergenic 40.0%
GGGGGAAACGTAATAAGTAG+GGG + chr3_1:67130223-67130242 None:intergenic 45.0%
GAGGGGGAAACGTAATAAGT+AGG + chr3_1:67130225-67130244 None:intergenic 45.0%
AGAGAGAGATGACATGTGAG+GGG + chr3_1:67130242-67130261 None:intergenic 45.0%
GAGAGAGAGATGACATGTGA+GGG + chr3_1:67130243-67130262 None:intergenic 45.0%
AGAGAGAGAGATGACATGTG+AGG + chr3_1:67130244-67130263 None:intergenic 45.0%
AGTGTAGCAGAGGTTCAGTT+TGG + chr3_1:67130311-67130330 None:intergenic 45.0%
! TAGCTGAAAGAGGGAGTGTA+GGG + chr3_1:67130340-67130359 None:intergenic 45.0%
! ATAGCTGAAAGAGGGAGTGT+AGG + chr3_1:67130341-67130360 None:intergenic 45.0%
GGGACCCAAGATGATTAACA+AGG + chr3_1:67130770-67130789 None:intergenic 45.0%
! CTGGCTTGCAATCTTTGCAA+AGG + chr3_1:67130791-67130810 None:intergenic 45.0%
TTGCAAAGATTGCAAGCCAG+AGG - chr3_1:67130791-67130810 Msa0369810:intron 45.0%
ATTGCAAGCCAGAGGTAACT+TGG - chr3_1:67130799-67130818 Msa0369810:intron 45.0%
AAGCCAGAGGTAACTTGGTT+TGG - chr3_1:67130804-67130823 Msa0369810:intron 45.0%
TTGTTATGGCCTGTCATAGG+TGG - chr3_1:67131156-67131175 Msa0369810:CDS 45.0%
GAGCCAGGTAAAGAAATTGG+AGG - chr3_1:67131225-67131244 Msa0369810:intron 45.0%
GAAATTGGAGGTCTCTATCC+TGG - chr3_1:67131237-67131256 Msa0369810:intron 45.0%
AGTGCTTGACAATAGGAGTG+AGG - chr3_1:67131745-67131764 Msa0369810:CDS 45.0%
!!! GCAGAAGAACTTCTGGCTTT+TGG - chr3_1:67131812-67131831 Msa0369810:CDS 45.0%
GATCTCAAGAGGGGAAAATG+GGG - chr3_1:67131870-67131889 Msa0369810:CDS 45.0%
GGGGGAAACGTAATAAGTAG+GGG + chr3_1:67130223-67130242 None:intergenic 45.0%
GAGGGGGAAACGTAATAAGT+AGG + chr3_1:67130225-67130244 None:intergenic 45.0%
AGAGAGAGATGACATGTGAG+GGG + chr3_1:67130242-67130261 None:intergenic 45.0%
GAGAGAGAGATGACATGTGA+GGG + chr3_1:67130243-67130262 None:intergenic 45.0%
AGAGAGAGAGATGACATGTG+AGG + chr3_1:67130244-67130263 None:intergenic 45.0%
AGTGTAGCAGAGGTTCAGTT+TGG + chr3_1:67130311-67130330 None:intergenic 45.0%
! TAGCTGAAAGAGGGAGTGTA+GGG + chr3_1:67130340-67130359 None:intergenic 45.0%
! ATAGCTGAAAGAGGGAGTGT+AGG + chr3_1:67130341-67130360 None:intergenic 45.0%
GGGACCCAAGATGATTAACA+AGG + chr3_1:67130770-67130789 None:intergenic 45.0%
! CTGGCTTGCAATCTTTGCAA+AGG + chr3_1:67130791-67130810 None:intergenic 45.0%
TTGCAAAGATTGCAAGCCAG+AGG - chr3_1:67130791-67130810 Msa0369810:intron 45.0%
ATTGCAAGCCAGAGGTAACT+TGG - chr3_1:67130799-67130818 Msa0369810:intron 45.0%
AAGCCAGAGGTAACTTGGTT+TGG - chr3_1:67130804-67130823 Msa0369810:intron 45.0%
TTGTTATGGCCTGTCATAGG+TGG - chr3_1:67131156-67131175 Msa0369810:CDS 45.0%
GAGCCAGGTAAAGAAATTGG+AGG - chr3_1:67131225-67131244 Msa0369810:intron 45.0%
GAAATTGGAGGTCTCTATCC+TGG - chr3_1:67131237-67131256 Msa0369810:intron 45.0%
AGTGCTTGACAATAGGAGTG+AGG - chr3_1:67131745-67131764 Msa0369810:CDS 45.0%
!!! GCAGAAGAACTTCTGGCTTT+TGG - chr3_1:67131812-67131831 Msa0369810:CDS 45.0%
GATCTCAAGAGGGGAAAATG+GGG - chr3_1:67131870-67131889 Msa0369810:CDS 45.0%
GAGAGAGATGACATGTGAGG+GGG + chr3_1:67130241-67130260 None:intergenic 50.0%
CTTCCACTTCCACCTATGAC+AGG + chr3_1:67131168-67131187 None:intergenic 50.0%
CTCATCACTGTGGCCAGTTT+AGG + chr3_1:67131208-67131227 None:intergenic 50.0%
CTTTACCTGGCTCATCACTG+TGG + chr3_1:67131218-67131237 None:intergenic 50.0%
GAGGTCTCTATCCTGGTACT+TGG - chr3_1:67131244-67131263 Msa0369810:intron 50.0%
! GCTTCTTTTGGCCAAGTACC+AGG + chr3_1:67131258-67131277 None:intergenic 50.0%
ATGGCTACCTTATCAGCACC+AGG - chr3_1:67131787-67131806 Msa0369810:CDS 50.0%
GAGAGAGATGACATGTGAGG+GGG + chr3_1:67130241-67130260 None:intergenic 50.0%
CTTCCACTTCCACCTATGAC+AGG + chr3_1:67131168-67131187 None:intergenic 50.0%
CTCATCACTGTGGCCAGTTT+AGG + chr3_1:67131208-67131227 None:intergenic 50.0%
CTTTACCTGGCTCATCACTG+TGG + chr3_1:67131218-67131237 None:intergenic 50.0%
GAGGTCTCTATCCTGGTACT+TGG - chr3_1:67131244-67131263 Msa0369810:intron 50.0%
! GCTTCTTTTGGCCAAGTACC+AGG + chr3_1:67131258-67131277 None:intergenic 50.0%
ATGGCTACCTTATCAGCACC+AGG - chr3_1:67131787-67131806 Msa0369810:CDS 50.0%
AGGGAGCTAGAGTGTAGCAG+AGG + chr3_1:67130321-67130340 None:intergenic 55.0%
TGGCCTGTCATAGGTGGAAG+TGG - chr3_1:67131162-67131181 Msa0369810:CDS 55.0%
CAGCAGCACAGAGGAGTTTG+TGG - chr3_1:67131701-67131720 Msa0369810:CDS 55.0%
GGAGTGAGGAAGAGAAGCCA+AGG - chr3_1:67131759-67131778 Msa0369810:CDS 55.0%
GCTACCTTATCAGCACCAGG+TGG - chr3_1:67131790-67131809 Msa0369810:CDS 55.0%
!! TCTGCCACCTGGTGCTGATA+AGG + chr3_1:67131797-67131816 None:intergenic 55.0%
CCAGAAGTTCTTCTGCCACC+TGG + chr3_1:67131808-67131827 None:intergenic 55.0%
CCAGGTGGCAGAAGAACTTC+TGG - chr3_1:67131805-67131824 Msa0369810:CDS 55.0%
AGGGAGCTAGAGTGTAGCAG+AGG + chr3_1:67130321-67130340 None:intergenic 55.0%
TGGCCTGTCATAGGTGGAAG+TGG - chr3_1:67131162-67131181 Msa0369810:CDS 55.0%
CAGCAGCACAGAGGAGTTTG+TGG - chr3_1:67131701-67131720 Msa0369810:CDS 55.0%
GGAGTGAGGAAGAGAAGCCA+AGG - chr3_1:67131759-67131778 Msa0369810:CDS 55.0%
GCTACCTTATCAGCACCAGG+TGG - chr3_1:67131790-67131809 Msa0369810:CDS 55.0%
!! TCTGCCACCTGGTGCTGATA+AGG + chr3_1:67131797-67131816 None:intergenic 55.0%
CCAGAAGTTCTTCTGCCACC+TGG + chr3_1:67131808-67131827 None:intergenic 55.0%
CCAGGTGGCAGAAGAACTTC+TGG - chr3_1:67131805-67131824 Msa0369810:CDS 55.0%
ACTGGCCACAGTGATGAGCC+AGG - chr3_1:67131210-67131229 Msa0369810:intron 60.0%
ACTGGCCACAGTGATGAGCC+AGG - chr3_1:67131210-67131229 Msa0369810:intron 60.0%
CAGCAGCTGCAGCAGCACAG+AGG - chr3_1:67131692-67131711 Msa0369810:CDS 65.0%
CAGCAGCTGCAGCAGCACAG+AGG - chr3_1:67131692-67131711 Msa0369810:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 67130181 67132191 67130181 ID=Msa0369810;Name=Msa0369810
chr3_1 mRNA 67130181 67132191 67130181 ID=Msa0369810-mRNA-1;Parent=Msa0369810;Name=Msa0369810-mRNA-1;_AED=0.05;_eAED=0.05;_QI=242|1|1|1|1|1|3|352|224
chr3_1 exon 67130181 67130695 67130181 ID=Msa0369810-mRNA-1:exon:13801;Parent=Msa0369810-mRNA-1
chr3_1 exon 67131076 67131197 67131076 ID=Msa0369810-mRNA-1:exon:13800;Parent=Msa0369810-mRNA-1
chr3_1 exon 67131560 67132191 67131560 ID=Msa0369810-mRNA-1:exon:13799;Parent=Msa0369810-mRNA-1
chr3_1 five_prime_UTR 67131950 67132191 67131950 ID=Msa0369810-mRNA-1:five_prime_utr;Parent=Msa0369810-mRNA-1
chr3_1 CDS 67131560 67131949 67131560 ID=Msa0369810-mRNA-1:cds;Parent=Msa0369810-mRNA-1
chr3_1 CDS 67131076 67131197 67131076 ID=Msa0369810-mRNA-1:cds;Parent=Msa0369810-mRNA-1
chr3_1 CDS 67130533 67130695 67130533 ID=Msa0369810-mRNA-1:cds;Parent=Msa0369810-mRNA-1
chr3_1 three_prime_UTR 67130181 67130532 67130181 ID=Msa0369810-mRNA-1:three_prime_utr;Parent=Msa0369810-mRNA-1
Gene Sequence

>Msa0369810

ATGCCAAGTTCAGCTTCATTTTTGAGGCAGTTAAGTTCAAAGGAGACTTGGAAATCAACATCAAATAGGTGGAGTGGAAAAAACACATACAACAACAATAGTTTAGGTGTGACTTGTGAGACAAATCTGAGTCAAATGGAAGGGTTTAACATTAACATGTATGGAAATGAAGAGAGTTGTGGAATGATGGTGAGAAAGAGAGTTATGGTTGTGGTTGATGGTACTTCACATTCTAAGCATGCAATGATTTGGGCACTTACTCATGTTGTTAATAAGGGTGATTTGTTGACTTTGCTTTACATTGTTTCTCCTCAAAGTGCTTCTGAGTCTTATTCTTCTACTTACCTTGTTAATCATCTTGGGTCCCTTTGCAAAGATTGCAAGCCAGAGGTGGAAGTGGAAGCACTTGTAATTCAAGGACCTAAACTGGCCACAGTGATGAGCCAGGTAAAGAAATTGGAGGTCTCTATCCTGGTACTTGGCCAAAAGAAGCCATCTTCTTTCTTTAGCTGTCTCTGTGGAAGTAGCAGCAGCTGCAGCAGCACAGAGGAGTTTGTGGAGTACTGTATCAACAATGCTGAGTGCTTGACAATAGGAGTGAGGAAGAGAAGCCAAGGCAATAATGGCTACCTTATCAGCACCAGGTGGCAGAAGAACTTCTGGCTTTTGGCTTAG
Protein sequence

>Msa0369810

MPSSASFLRQLSSKETWKSTSNRWSGKNTYNNNSLGVTCETNLSQMEGFNINMYGNEESCGMMVRKRVMVVVDGTSHSKHAMIWALTHVVNKGDLLTLLYIVSPQSASESYSSTYLVNHLGSLCKDCKPEVEVEALVIQGPKLATVMSQVKKLEVSILVLGQKKPSSFFSCLCGSSSSCSSTEEFVEYCINNAECLTIGVRKRSQGNNGYLISTRWQKNFWLLA*