AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0509570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0509570 MtrunA17_Chr3g0114601 98.661 224 3 0 1 224 1 224 4.22e-166 456
Msa0509570 MtrunA17_Chr5g0420141 35.795 176 100 4 61 224 62 236 4.44e-30 111
Msa0509570 MtrunA17_Chr4g0061701 34.545 165 100 3 67 224 59 222 1.17e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0509570 AT1G44760.1 58.850 226 73 6 3 224 4 213 8.88e-83 246
Msa0509570 AT1G44760.2 58.850 226 73 6 3 224 75 284 2.07e-81 244
Msa0509570 AT2G03720.2 38.122 181 99 5 56 224 53 232 1.14e-30 113
Msa0509570 AT2G03720.1 39.157 166 90 4 69 224 1 165 1.42e-29 108
Msa0509570 AT1G69080.1 32.222 180 101 3 66 224 44 223 1.96e-28 107
Msa0509570 AT5G17390.1 36.310 168 97 3 67 224 118 285 2.59e-26 103
Msa0509570 AT1G69080.2 34.706 170 96 5 66 224 44 209 5.26e-26 100
Msa0509570 AT3G03290.1 34.503 171 96 4 67 224 107 274 1.51e-23 95.5
Msa0509570 AT4G13450.1 25.728 206 105 4 66 224 15 219 7.86e-16 73.9

Find 84 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTACTCATGTTGTTAATA+AGG 0.194266 3_4:-58002962 Msa0509570:CDS
GCAGAAGAACTTCTGGCTTT+TGG 0.227711 3_4:-58001818 Msa0509570:CDS
CATTTGTGGTTGCATAAATA+AGG 0.255308 3_4:-58003258 Msa0509570:five_prime_UTR
AGATTATTACTTACTATAGA+TGG 0.287966 3_4:+58003355 None:intergenic
ATTCTAAGCATGCAATGATT+TGG 0.290584 3_4:-58002987 Msa0509570:CDS
TAAAGTGTCACGGGAATCTT+TGG 0.317138 3_4:+58003474 None:intergenic
TAGATCTCAAGAGGGGAAAA+TGG 0.321677 3_4:-58001762 Msa0509570:three_prime_UTR
CTCATCACTGTGGCCAGTTT+AGG 0.329869 3_4:+58002425 None:intergenic
ACTTACCTTGTTAATCATCT+TGG 0.331349 3_4:-58002877 Msa0509570:CDS
AGAAAGAAGATGGCTTCTTT+TGG 0.334209 3_4:+58002363 None:intergenic
GATGAGCCAGGTAAAGAAAT+TGG 0.339137 3_4:-58002408 Msa0509570:CDS
CCAGGTGGCAGAAGAACTTC+TGG 0.346204 3_4:-58001825 Msa0509570:CDS
CTTACCTTGTTAATCATCTT+GGG 0.346303 3_4:-58002876 Msa0509570:CDS
TTCTAAGCATGCAATGATTT+GGG 0.371229 3_4:-58002986 Msa0509570:CDS
CTTACTCATGTTGTTAATAA+GGG 0.375126 3_4:-58002961 Msa0509570:CDS
ACATACAACAACAATAGTTT+AGG 0.386222 3_4:-58003132 Msa0509570:CDS
GGGTTTAACATTAACATGTA+TGG 0.388232 3_4:-58003075 Msa0509570:CDS
CTTGGAAATCAACATCAAAT+AGG 0.391956 3_4:-58003170 Msa0509570:CDS
AAGAGAAGCCAAGGCAATAA+TGG 0.394438 3_4:-58001862 Msa0509570:CDS
AGCCATCTTCTTTCTTTAGC+TGG 0.402605 3_4:-58002355 Msa0509570:intron
AGATCTCAAGAGGGGAAAAT+GGG 0.404854 3_4:-58001761 Msa0509570:three_prime_UTR
AGCAATAATAGTTACATTTG+TGG 0.408337 3_4:-58003272 Msa0509570:five_prime_UTR
TGTAATTCAAGGACCTAAAC+TGG 0.413139 3_4:-58002438 Msa0509570:CDS
CAGCAGCACAGAGGAGTTTG+TGG 0.433556 3_4:-58001929 Msa0509570:CDS
TCTGCCACCTGGTGCTGATA+AGG 0.435349 3_4:+58001836 None:intergenic
AGAGTTATGGTAGTGGTTGA+TGG 0.444052 3_4:-58003018 Msa0509570:CDS
AATGCTGAGTGCTTGACAAT+AGG 0.444116 3_4:-58001892 Msa0509570:CDS
GAGGGGAAACGTAATAAGTA+GGG 0.445122 3_4:+58003431 None:intergenic
TAGCTGAAAGAGGGAGTGTA+GGG 0.448424 3_4:+58003302 None:intergenic
TGAGACAAATCTGAGTCAAA+TGG 0.450642 3_4:-58003100 Msa0509570:CDS
TAAGGTAGCCATTATTGCCT+TGG 0.456289 3_4:+58001854 None:intergenic
TAAGACTCAGAAGCACTTTG+AGG 0.458252 3_4:+58002907 None:intergenic
AAAATATTATTCACTAGCAT+TGG 0.464290 3_4:+58001567 None:intergenic
AGTGTAGCAGAGGTTCAGTT+TGG 0.464785 3_4:+58003331 None:intergenic
ATAGCTGAAAGAGGGAGTGT+AGG 0.471533 3_4:+58003301 None:intergenic
GATGGTGAGAAAGAGAGTTA+TGG 0.480958 3_4:-58003031 Msa0509570:CDS
TGGCTTGCAATCTTTGCAAA+GGG 0.487254 3_4:+58002852 None:intergenic
AGAGGGGAAACGTAATAAGT+AGG 0.489959 3_4:+58003430 None:intergenic
ATTCACTAGCATTGGCAAGT+TGG 0.496266 3_4:+58001575 None:intergenic
GAGAGAGAGGACATGTGAGA+GGG 0.499081 3_4:+58003413 None:intergenic
GAAATTGGAGGTCTCTATCC+TGG 0.499739 3_4:-58002393 Msa0509570:CDS
TATGGGTAAAGATTATTCTC+TGG 0.503481 3_4:-58001535 Msa0509570:three_prime_UTR
GTGGAAGCACTTGTAATTCA+AGG 0.506797 3_4:-58002449 Msa0509570:CDS
AGACCTCCAATTTCTTTACC+TGG 0.507576 3_4:+58002402 None:intergenic
CCAGAAGTTCTTCTGCCACC+TGG 0.508759 3_4:+58001825 None:intergenic
AGAGAGAGGACATGTGAGAG+GGG 0.509415 3_4:+58003414 None:intergenic
TGGCCTGTCATAGGTGGAAG+TGG 0.514126 3_4:-58002468 Msa0509570:intron
TGTGCAGATTAAAGTGTCAC+GGG 0.525016 3_4:+58003465 None:intergenic
TTTGAGGCAGTTAAGTTCAA+AGG 0.525241 3_4:-58003196 Msa0509570:CDS
CTGGCTTGCAATCTTTGCAA+AGG 0.528141 3_4:+58002851 None:intergenic
TGAAGAGAGTTGTGGAATGA+TGG 0.529080 3_4:-58003049 Msa0509570:CDS
GATTATTACTTACTATAGAT+GGG 0.534676 3_4:+58003356 None:intergenic
GAGGTCTCTATCCTGGTACT+TGG 0.534821 3_4:-58002386 Msa0509570:CDS
AGAGAGAGAGGACATGTGAG+AGG 0.538275 3_4:+58003412 None:intergenic
TACCAGCTAAAGAAAGAAGA+TGG 0.539360 3_4:+58002353 None:intergenic
AGTTAAGTTCAAAGGAGACT+TGG 0.544950 3_4:-58003188 Msa0509570:CDS
TTGTGCAGATTAAAGTGTCA+CGG 0.548162 3_4:+58003464 None:intergenic
GCTAGTTGAATAGCTGAAAG+AGG 0.552730 3_4:+58003292 None:intergenic
AGATGAGAATACAAATATCA+AGG 0.553739 3_4:+58001726 None:intergenic
ACTGGCCACAGTGATGAGCC+AGG 0.573013 3_4:-58002420 Msa0509570:CDS
TCAACATCAAATAGGTGGAG+TGG 0.576570 3_4:-58003162 Msa0509570:CDS
ACAAATCTGAGTCAAATGGA+AGG 0.584700 3_4:-58003096 Msa0509570:CDS
TACTAGTCTTAGATCTCAAG+AGG 0.588812 3_4:-58001771 Msa0509570:three_prime_UTR
CTGTTTGTTTACAGTCTCTG+TGG 0.592127 3_4:-58001967 Msa0509570:intron
GAGAAAGAGAGTTATGGTAG+TGG 0.598245 3_4:-58003025 Msa0509570:CDS
GGAGTGAGGAAGAGAAGCCA+AGG 0.601340 3_4:-58001871 Msa0509570:CDS
CTAGTTGAATAGCTGAAAGA+GGG 0.601553 3_4:+58003293 None:intergenic
CAAATCTGAGTCAAATGGAA+GGG 0.609381 3_4:-58003095 Msa0509570:CDS
GGAAATCAACATCAAATAGG+TGG 0.613048 3_4:-58003167 Msa0509570:CDS
ACTAGTCTTAGATCTCAAGA+GGG 0.615258 3_4:-58001770 Msa0509570:three_prime_UTR
CAGCAGCTGCAGCAGCACAG+AGG 0.618197 3_4:-58001938 Msa0509570:CDS
ATGGCTACCTTATCAGCACC+AGG 0.619844 3_4:-58001843 Msa0509570:CDS
TATGGAAATGAAGAGAGTTG+TGG 0.622956 3_4:-58003057 Msa0509570:CDS
GAGCCAGGTAAAGAAATTGG+AGG 0.635543 3_4:-58002405 Msa0509570:CDS
GATCTCAAGAGGGGAAAATG+GGG 0.639595 3_4:-58001760 Msa0509570:three_prime_UTR
TTGCAAAGATTGCAAGCCAG+AGG 0.640746 3_4:-58002848 Msa0509570:intron
AGGGAGCTAGAGTGTAGCAG+AGG 0.656163 3_4:+58003321 None:intergenic
CTTTACCTGGCTCATCACTG+TGG 0.657425 3_4:+58002415 None:intergenic
GGGACCCAAGATGATTAACA+AGG 0.679967 3_4:+58002872 None:intergenic
AGGGGAAACGTAATAAGTAG+GGG 0.680654 3_4:+58003432 None:intergenic
AGTGCTTGACAATAGGAGTG+AGG 0.688153 3_4:-58001885 Msa0509570:CDS
GGTTGCATAAATAAGGCACA+AGG 0.702121 3_4:-58003251 Msa0509570:five_prime_UTR
CTAGTCTTAGATCTCAAGAG+GGG 0.706285 3_4:-58001769 Msa0509570:three_prime_UTR
GCTACCTTATCAGCACCAGG+TGG 0.765926 3_4:-58001840 Msa0509570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGTAGGAAAATAATTTA+TGG + chr3_4:58002296-58002315 None:intergenic 15.0%
!!! TTTTTTAAAACAAGACAATT+GGG + chr3_4:58002853-58002872 None:intergenic 15.0%
!!! ATTTTTTAAAACAAGACAAT+TGG + chr3_4:58002854-58002873 None:intergenic 15.0%
!!! TAATATTTTTACTCATGTTA+TGG - chr3_4:58003379-58003398 Msa0509570:five_prime_UTR 15.0%
!!! AATATTTTTACTCATGTTAT+GGG - chr3_4:58003380-58003399 Msa0509570:five_prime_UTR 15.0%
!! AAAAGTAGGAAAATAATTTA+TGG + chr3_4:58002296-58002315 None:intergenic 15.0%
!!! TTTTTTAAAACAAGACAATT+GGG + chr3_4:58002853-58002872 None:intergenic 15.0%
!!! ATTTTTTAAAACAAGACAAT+TGG + chr3_4:58002854-58002873 None:intergenic 15.0%
!!! TAATATTTTTACTCATGTTA+TGG - chr3_4:58003379-58003398 Msa0509570:five_prime_UTR 15.0%
!!! AATATTTTTACTCATGTTAT+GGG - chr3_4:58003380-58003399 Msa0509570:five_prime_UTR 15.0%
!! GATTATTACTTACTATAGAT+GGG + chr3_4:58001579-58001598 None:intergenic 20.0%
!! AGATTATTACTTACTATAGA+TGG + chr3_4:58001580-58001599 None:intergenic 20.0%
!!! TTGTTCAATTGATAGTTAAT+TGG - chr3_4:58002208-58002227 Msa0509570:intron 20.0%
!! ATTAGTTGAAAAGAGAAAAA+GGG + chr3_4:58002263-58002282 None:intergenic 20.0%
!! AATTAGTTGAAAAGAGAAAA+AGG + chr3_4:58002264-58002283 None:intergenic 20.0%
!! AATTTACTATTGTCAATTAG+AGG + chr3_4:58002794-58002813 None:intergenic 20.0%
!!! TTTTTAAAACAAGACAATTG+GGG + chr3_4:58002852-58002871 None:intergenic 20.0%
!! AAAATATTATTCACTAGCAT+TGG + chr3_4:58003368-58003387 None:intergenic 20.0%
!! GATTATTACTTACTATAGAT+GGG + chr3_4:58001579-58001598 None:intergenic 20.0%
!! AGATTATTACTTACTATAGA+TGG + chr3_4:58001580-58001599 None:intergenic 20.0%
!!! TTGTTCAATTGATAGTTAAT+TGG - chr3_4:58002208-58002227 Msa0509570:intron 20.0%
!! ATTAGTTGAAAAGAGAAAAA+GGG + chr3_4:58002263-58002282 None:intergenic 20.0%
!! AATTAGTTGAAAAGAGAAAA+AGG + chr3_4:58002264-58002283 None:intergenic 20.0%
!! AATTTACTATTGTCAATTAG+AGG + chr3_4:58002794-58002813 None:intergenic 20.0%
!!! TTTTTAAAACAAGACAATTG+GGG + chr3_4:58002852-58002871 None:intergenic 20.0%
!! AAAATATTATTCACTAGCAT+TGG + chr3_4:58003368-58003387 None:intergenic 20.0%
! AGCAATAATAGTTACATTTG+TGG - chr3_4:58001660-58001679 Msa0509570:three_prime_UTR 25.0%
! ACATACAACAACAATAGTTT+AGG - chr3_4:58001800-58001819 Msa0509570:exon 25.0%
! ACTTACTCATGTTGTTAATA+AGG - chr3_4:58001970-58001989 Msa0509570:intron 25.0%
! CTTACTCATGTTGTTAATAA+GGG - chr3_4:58001971-58001990 Msa0509570:intron 25.0%
! GATAGTTAATTGGAGTAATT+AGG - chr3_4:58002218-58002237 Msa0509570:intron 25.0%
! CATCTAAAGAATAGAAAAGT+AGG + chr3_4:58002310-58002329 None:intergenic 25.0%
!!! CTACTTTTCTATTCTTTAGA+TGG - chr3_4:58002308-58002327 Msa0509570:intron 25.0%
! GAAAAGCTGAAAATAAAACA+AGG + chr3_4:58002369-58002388 None:intergenic 25.0%
! TTTCTTTGTATTGAGGATAA+GGG + chr3_4:58002425-58002444 None:intergenic 25.0%
!! TTTTCTTTGTATTGAGGATA+AGG + chr3_4:58002426-58002445 None:intergenic 25.0%
! TATCCTCAATACAAAGAAAA+AGG - chr3_4:58002426-58002445 Msa0509570:CDS 25.0%
!! TAACCTTTTTCTTTGTATTG+AGG + chr3_4:58002432-58002451 None:intergenic 25.0%
! AAAGGTTACTGATATTGTTA+TGG - chr3_4:58002444-58002463 Msa0509570:CDS 25.0%
!!! CAAAGTTTTTCTTGTTACTT+GGG - chr3_4:58002899-58002918 Msa0509570:CDS 25.0%
! AGATGAGAATACAAATATCA+AGG + chr3_4:58003209-58003228 None:intergenic 25.0%
!!! TAACAAGTCTTGTCATTTTA+AGG - chr3_4:58003295-58003314 Msa0509570:five_prime_UTR 25.0%
! AGCAATAATAGTTACATTTG+TGG - chr3_4:58001660-58001679 Msa0509570:three_prime_UTR 25.0%
! ACATACAACAACAATAGTTT+AGG - chr3_4:58001800-58001819 Msa0509570:exon 25.0%
! ACTTACTCATGTTGTTAATA+AGG - chr3_4:58001970-58001989 Msa0509570:intron 25.0%
! CTTACTCATGTTGTTAATAA+GGG - chr3_4:58001971-58001990 Msa0509570:intron 25.0%
! GATAGTTAATTGGAGTAATT+AGG - chr3_4:58002218-58002237 Msa0509570:intron 25.0%
! CATCTAAAGAATAGAAAAGT+AGG + chr3_4:58002310-58002329 None:intergenic 25.0%
!!! CTACTTTTCTATTCTTTAGA+TGG - chr3_4:58002308-58002327 Msa0509570:intron 25.0%
! GAAAAGCTGAAAATAAAACA+AGG + chr3_4:58002369-58002388 None:intergenic 25.0%
! TTTCTTTGTATTGAGGATAA+GGG + chr3_4:58002425-58002444 None:intergenic 25.0%
!! TTTTCTTTGTATTGAGGATA+AGG + chr3_4:58002426-58002445 None:intergenic 25.0%
! TATCCTCAATACAAAGAAAA+AGG - chr3_4:58002426-58002445 Msa0509570:CDS 25.0%
!! TAACCTTTTTCTTTGTATTG+AGG + chr3_4:58002432-58002451 None:intergenic 25.0%
! AAAGGTTACTGATATTGTTA+TGG - chr3_4:58002444-58002463 Msa0509570:CDS 25.0%
!!! CAAAGTTTTTCTTGTTACTT+GGG - chr3_4:58002899-58002918 Msa0509570:CDS 25.0%
! AGATGAGAATACAAATATCA+AGG + chr3_4:58003209-58003228 None:intergenic 25.0%
!!! TAACAAGTCTTGTCATTTTA+AGG - chr3_4:58003295-58003314 Msa0509570:five_prime_UTR 25.0%
CATTTGTGGTTGCATAAATA+AGG - chr3_4:58001674-58001693 Msa0509570:three_prime_UTR 30.0%
CTTGGAAATCAACATCAAAT+AGG - chr3_4:58001762-58001781 Msa0509570:three_prime_UTR 30.0%
GGGTTTAACATTAACATGTA+TGG - chr3_4:58001857-58001876 Msa0509570:CDS 30.0%
ATTCTAAGCATGCAATGATT+TGG - chr3_4:58001945-58001964 Msa0509570:CDS 30.0%
TTCTAAGCATGCAATGATTT+GGG - chr3_4:58001946-58001965 Msa0509570:CDS 30.0%
ACTTACCTTGTTAATCATCT+TGG - chr3_4:58002055-58002074 Msa0509570:intron 30.0%
CTTACCTTGTTAATCATCTT+GGG - chr3_4:58002056-58002075 Msa0509570:intron 30.0%
TTCTTTGTATTGAGGATAAG+GGG + chr3_4:58002424-58002443 None:intergenic 30.0%
TGTTGTAAATTGCTTGTGTT+TGG + chr3_4:58002618-58002637 None:intergenic 30.0%
! CAATGTACAATTTTCAACAG+AGG + chr3_4:58002698-58002717 None:intergenic 30.0%
! TTTGGAAGTATGACATCAAA+AGG + chr3_4:58002883-58002902 None:intergenic 30.0%
CCAAGTAACAAGAAAAACTT+TGG + chr3_4:58002901-58002920 None:intergenic 30.0%
! CCAAAGTTTTTCTTGTTACT+TGG - chr3_4:58002898-58002917 Msa0509570:CDS 30.0%
!!! TTTTAAGGCACTATGGTAAT+TGG - chr3_4:58003310-58003329 Msa0509570:five_prime_UTR 30.0%
! GTGATTTTCAAACATGTAGT+TGG + chr3_4:58003338-58003357 None:intergenic 30.0%
TATGGGTAAAGATTATTCTC+TGG - chr3_4:58003397-58003416 Msa0509570:five_prime_UTR 30.0%
CATTTGTGGTTGCATAAATA+AGG - chr3_4:58001674-58001693 Msa0509570:three_prime_UTR 30.0%
CTTGGAAATCAACATCAAAT+AGG - chr3_4:58001762-58001781 Msa0509570:three_prime_UTR 30.0%
GGGTTTAACATTAACATGTA+TGG - chr3_4:58001857-58001876 Msa0509570:CDS 30.0%
ATTCTAAGCATGCAATGATT+TGG - chr3_4:58001945-58001964 Msa0509570:CDS 30.0%
TTCTAAGCATGCAATGATTT+GGG - chr3_4:58001946-58001965 Msa0509570:CDS 30.0%
ACTTACCTTGTTAATCATCT+TGG - chr3_4:58002055-58002074 Msa0509570:intron 30.0%
CTTACCTTGTTAATCATCTT+GGG - chr3_4:58002056-58002075 Msa0509570:intron 30.0%
TTCTTTGTATTGAGGATAAG+GGG + chr3_4:58002424-58002443 None:intergenic 30.0%
TGTTGTAAATTGCTTGTGTT+TGG + chr3_4:58002618-58002637 None:intergenic 30.0%
! CAATGTACAATTTTCAACAG+AGG + chr3_4:58002698-58002717 None:intergenic 30.0%
! TTTGGAAGTATGACATCAAA+AGG + chr3_4:58002883-58002902 None:intergenic 30.0%
CCAAGTAACAAGAAAAACTT+TGG + chr3_4:58002901-58002920 None:intergenic 30.0%
! CCAAAGTTTTTCTTGTTACT+TGG - chr3_4:58002898-58002917 Msa0509570:CDS 30.0%
!!! TTTTAAGGCACTATGGTAAT+TGG - chr3_4:58003310-58003329 Msa0509570:five_prime_UTR 30.0%
! GTGATTTTCAAACATGTAGT+TGG + chr3_4:58003338-58003357 None:intergenic 30.0%
TATGGGTAAAGATTATTCTC+TGG - chr3_4:58003397-58003416 Msa0509570:five_prime_UTR 30.0%
! TTGTGCAGATTAAAGTGTCA+CGG + chr3_4:58001471-58001490 None:intergenic 35.0%
CTAGTTGAATAGCTGAAAGA+GGG + chr3_4:58001642-58001661 None:intergenic 35.0%
CTCAAAAATGAAGCTGAACT+TGG + chr3_4:58001722-58001741 None:intergenic 35.0%
!! CAAGTTCAGCTTCATTTTTG+AGG - chr3_4:58001720-58001739 Msa0509570:three_prime_UTR 35.0%
! TTTGAGGCAGTTAAGTTCAA+AGG - chr3_4:58001736-58001755 Msa0509570:three_prime_UTR 35.0%
AGTTAAGTTCAAAGGAGACT+TGG - chr3_4:58001744-58001763 Msa0509570:three_prime_UTR 35.0%
GGAAATCAACATCAAATAGG+TGG - chr3_4:58001765-58001784 Msa0509570:three_prime_UTR 35.0%
TGAGACAAATCTGAGTCAAA+TGG - chr3_4:58001832-58001851 Msa0509570:CDS 35.0%
ACAAATCTGAGTCAAATGGA+AGG - chr3_4:58001836-58001855 Msa0509570:CDS 35.0%
CAAATCTGAGTCAAATGGAA+GGG - chr3_4:58001837-58001856 Msa0509570:CDS 35.0%
TATGGAAATGAAGAGAGTTG+TGG - chr3_4:58001875-58001894 Msa0509570:CDS 35.0%
TCTTTGTATTGAGGATAAGG+GGG + chr3_4:58002423-58002442 None:intergenic 35.0%
ATATTGTTATGGCCTGTCAT+AGG - chr3_4:58002455-58002474 Msa0509570:CDS 35.0%
TGTAATTCAAGGACCTAAAC+TGG - chr3_4:58002494-58002513 Msa0509570:intron 35.0%
! AGAAAGAAGATGGCTTCTTT+TGG + chr3_4:58002572-58002591 None:intergenic 35.0%
TACCAGCTAAAGAAAGAAGA+TGG + chr3_4:58002582-58002601 None:intergenic 35.0%
TCCATGATATGTCAGTTAAG+TGG - chr3_4:58002639-58002658 Msa0509570:intron 35.0%
TAGAGCTTGAACTTGTAAAC+AGG - chr3_4:58002664-58002683 Msa0509570:intron 35.0%
AGAGCTTGAACTTGTAAACA+GGG - chr3_4:58002665-58002684 Msa0509570:intron 35.0%
TACACTTAGCTGACATATCA+CGG + chr3_4:58002740-58002759 None:intergenic 35.0%
!! TACTAGTCTTAGATCTCAAG+AGG - chr3_4:58003161-58003180 Msa0509570:CDS 35.0%
!! ACTAGTCTTAGATCTCAAGA+GGG - chr3_4:58003162-58003181 Msa0509570:CDS 35.0%
!!! CTTGTCATTTTAAGGCACTA+TGG - chr3_4:58003303-58003322 Msa0509570:five_prime_UTR 35.0%
! TTGTGCAGATTAAAGTGTCA+CGG + chr3_4:58001471-58001490 None:intergenic 35.0%
CTAGTTGAATAGCTGAAAGA+GGG + chr3_4:58001642-58001661 None:intergenic 35.0%
CTCAAAAATGAAGCTGAACT+TGG + chr3_4:58001722-58001741 None:intergenic 35.0%
!! CAAGTTCAGCTTCATTTTTG+AGG - chr3_4:58001720-58001739 Msa0509570:three_prime_UTR 35.0%
! TTTGAGGCAGTTAAGTTCAA+AGG - chr3_4:58001736-58001755 Msa0509570:three_prime_UTR 35.0%
AGTTAAGTTCAAAGGAGACT+TGG - chr3_4:58001744-58001763 Msa0509570:three_prime_UTR 35.0%
GGAAATCAACATCAAATAGG+TGG - chr3_4:58001765-58001784 Msa0509570:three_prime_UTR 35.0%
TGAGACAAATCTGAGTCAAA+TGG - chr3_4:58001832-58001851 Msa0509570:CDS 35.0%
ACAAATCTGAGTCAAATGGA+AGG - chr3_4:58001836-58001855 Msa0509570:CDS 35.0%
CAAATCTGAGTCAAATGGAA+GGG - chr3_4:58001837-58001856 Msa0509570:CDS 35.0%
TATGGAAATGAAGAGAGTTG+TGG - chr3_4:58001875-58001894 Msa0509570:CDS 35.0%
TCTTTGTATTGAGGATAAGG+GGG + chr3_4:58002423-58002442 None:intergenic 35.0%
ATATTGTTATGGCCTGTCAT+AGG - chr3_4:58002455-58002474 Msa0509570:CDS 35.0%
TGTAATTCAAGGACCTAAAC+TGG - chr3_4:58002494-58002513 Msa0509570:intron 35.0%
! AGAAAGAAGATGGCTTCTTT+TGG + chr3_4:58002572-58002591 None:intergenic 35.0%
TACCAGCTAAAGAAAGAAGA+TGG + chr3_4:58002582-58002601 None:intergenic 35.0%
TCCATGATATGTCAGTTAAG+TGG - chr3_4:58002639-58002658 Msa0509570:intron 35.0%
TAGAGCTTGAACTTGTAAAC+AGG - chr3_4:58002664-58002683 Msa0509570:intron 35.0%
AGAGCTTGAACTTGTAAACA+GGG - chr3_4:58002665-58002684 Msa0509570:intron 35.0%
TACACTTAGCTGACATATCA+CGG + chr3_4:58002740-58002759 None:intergenic 35.0%
!! TACTAGTCTTAGATCTCAAG+AGG - chr3_4:58003161-58003180 Msa0509570:CDS 35.0%
!! ACTAGTCTTAGATCTCAAGA+GGG - chr3_4:58003162-58003181 Msa0509570:CDS 35.0%
!!! CTTGTCATTTTAAGGCACTA+TGG - chr3_4:58003303-58003322 Msa0509570:five_prime_UTR 35.0%
! TGTGCAGATTAAAGTGTCAC+GGG + chr3_4:58001470-58001489 None:intergenic 40.0%
AGGGGAAACGTAATAAGTAG+GGG + chr3_4:58001503-58001522 None:intergenic 40.0%
GAGGGGAAACGTAATAAGTA+GGG + chr3_4:58001504-58001523 None:intergenic 40.0%
AGAGGGGAAACGTAATAAGT+AGG + chr3_4:58001505-58001524 None:intergenic 40.0%
GCTAGTTGAATAGCTGAAAG+AGG + chr3_4:58001643-58001662 None:intergenic 40.0%
GGTTGCATAAATAAGGCACA+AGG - chr3_4:58001681-58001700 Msa0509570:three_prime_UTR 40.0%
TCAACATCAAATAGGTGGAG+TGG - chr3_4:58001770-58001789 Msa0509570:three_prime_UTR 40.0%
TGAAGAGAGTTGTGGAATGA+TGG - chr3_4:58001883-58001902 Msa0509570:CDS 40.0%
GATGGTGAGAAAGAGAGTTA+TGG - chr3_4:58001901-58001920 Msa0509570:CDS 40.0%
GAGAAAGAGAGTTATGGTAG+TGG - chr3_4:58001907-58001926 Msa0509570:CDS 40.0%
!! AGAGTTATGGTAGTGGTTGA+TGG - chr3_4:58001914-58001933 Msa0509570:CDS 40.0%
!! TAAGACTCAGAAGCACTTTG+AGG + chr3_4:58002028-58002047 None:intergenic 40.0%
! TGGCTTGCAATCTTTGCAAA+GGG + chr3_4:58002083-58002102 None:intergenic 40.0%
AAACCAAACCAAGTTACCTC+TGG + chr3_4:58002103-58002122 None:intergenic 40.0%
!! GTGGAAGCACTTGTAATTCA+AGG - chr3_4:58002483-58002502 Msa0509570:intron 40.0%
GATGAGCCAGGTAAAGAAAT+TGG - chr3_4:58002524-58002543 Msa0509570:intron 40.0%
AGACCTCCAATTTCTTTACC+TGG + chr3_4:58002533-58002552 None:intergenic 40.0%
AGCCATCTTCTTTCTTTAGC+TGG - chr3_4:58002577-58002596 Msa0509570:intron 40.0%
GCCACTTAACTGACATATCA+TGG + chr3_4:58002643-58002662 None:intergenic 40.0%
AAACAAGACAATTGGGGTGA+TGG + chr3_4:58002846-58002865 None:intergenic 40.0%
CTGTTTGTTTACAGTCTCTG+TGG - chr3_4:58002965-58002984 Msa0509570:CDS 40.0%
AATGCTGAGTGCTTGACAAT+AGG - chr3_4:58003040-58003059 Msa0509570:CDS 40.0%
AAGAGAAGCCAAGGCAATAA+TGG - chr3_4:58003070-58003089 Msa0509570:CDS 40.0%
TAAGGTAGCCATTATTGCCT+TGG + chr3_4:58003081-58003100 None:intergenic 40.0%
!! CTAGTCTTAGATCTCAAGAG+GGG - chr3_4:58003163-58003182 Msa0509570:CDS 40.0%
TAGATCTCAAGAGGGGAAAA+TGG - chr3_4:58003170-58003189 Msa0509570:CDS 40.0%
AGATCTCAAGAGGGGAAAAT+GGG - chr3_4:58003171-58003190 Msa0509570:CDS 40.0%
!! ATTCACTAGCATTGGCAAGT+TGG + chr3_4:58003360-58003379 None:intergenic 40.0%
! TGTGCAGATTAAAGTGTCAC+GGG + chr3_4:58001470-58001489 None:intergenic 40.0%
AGGGGAAACGTAATAAGTAG+GGG + chr3_4:58001503-58001522 None:intergenic 40.0%
GAGGGGAAACGTAATAAGTA+GGG + chr3_4:58001504-58001523 None:intergenic 40.0%
AGAGGGGAAACGTAATAAGT+AGG + chr3_4:58001505-58001524 None:intergenic 40.0%
GCTAGTTGAATAGCTGAAAG+AGG + chr3_4:58001643-58001662 None:intergenic 40.0%
GGTTGCATAAATAAGGCACA+AGG - chr3_4:58001681-58001700 Msa0509570:three_prime_UTR 40.0%
TCAACATCAAATAGGTGGAG+TGG - chr3_4:58001770-58001789 Msa0509570:three_prime_UTR 40.0%
TGAAGAGAGTTGTGGAATGA+TGG - chr3_4:58001883-58001902 Msa0509570:CDS 40.0%
GATGGTGAGAAAGAGAGTTA+TGG - chr3_4:58001901-58001920 Msa0509570:CDS 40.0%
GAGAAAGAGAGTTATGGTAG+TGG - chr3_4:58001907-58001926 Msa0509570:CDS 40.0%
!! AGAGTTATGGTAGTGGTTGA+TGG - chr3_4:58001914-58001933 Msa0509570:CDS 40.0%
!! TAAGACTCAGAAGCACTTTG+AGG + chr3_4:58002028-58002047 None:intergenic 40.0%
! TGGCTTGCAATCTTTGCAAA+GGG + chr3_4:58002083-58002102 None:intergenic 40.0%
AAACCAAACCAAGTTACCTC+TGG + chr3_4:58002103-58002122 None:intergenic 40.0%
!! GTGGAAGCACTTGTAATTCA+AGG - chr3_4:58002483-58002502 Msa0509570:intron 40.0%
GATGAGCCAGGTAAAGAAAT+TGG - chr3_4:58002524-58002543 Msa0509570:intron 40.0%
AGACCTCCAATTTCTTTACC+TGG + chr3_4:58002533-58002552 None:intergenic 40.0%
AGCCATCTTCTTTCTTTAGC+TGG - chr3_4:58002577-58002596 Msa0509570:intron 40.0%
GCCACTTAACTGACATATCA+TGG + chr3_4:58002643-58002662 None:intergenic 40.0%
AAACAAGACAATTGGGGTGA+TGG + chr3_4:58002846-58002865 None:intergenic 40.0%
CTGTTTGTTTACAGTCTCTG+TGG - chr3_4:58002965-58002984 Msa0509570:CDS 40.0%
AATGCTGAGTGCTTGACAAT+AGG - chr3_4:58003040-58003059 Msa0509570:CDS 40.0%
AAGAGAAGCCAAGGCAATAA+TGG - chr3_4:58003070-58003089 Msa0509570:CDS 40.0%
TAAGGTAGCCATTATTGCCT+TGG + chr3_4:58003081-58003100 None:intergenic 40.0%
!! CTAGTCTTAGATCTCAAGAG+GGG - chr3_4:58003163-58003182 Msa0509570:CDS 40.0%
TAGATCTCAAGAGGGGAAAA+TGG - chr3_4:58003170-58003189 Msa0509570:CDS 40.0%
AGATCTCAAGAGGGGAAAAT+GGG - chr3_4:58003171-58003190 Msa0509570:CDS 40.0%
!! ATTCACTAGCATTGGCAAGT+TGG + chr3_4:58003360-58003379 None:intergenic 40.0%
AGTGTAGCAGAGGTTCAGTT+TGG + chr3_4:58001604-58001623 None:intergenic 45.0%
! TAGCTGAAAGAGGGAGTGTA+GGG + chr3_4:58001633-58001652 None:intergenic 45.0%
! ATAGCTGAAAGAGGGAGTGT+AGG + chr3_4:58001634-58001653 None:intergenic 45.0%
GGGACCCAAGATGATTAACA+AGG + chr3_4:58002063-58002082 None:intergenic 45.0%
! CTGGCTTGCAATCTTTGCAA+AGG + chr3_4:58002084-58002103 None:intergenic 45.0%
TTGCAAAGATTGCAAGCCAG+AGG - chr3_4:58002084-58002103 Msa0509570:intron 45.0%
ATTGCAAGCCAGAGGTAACT+TGG - chr3_4:58002092-58002111 Msa0509570:intron 45.0%
AAGCCAGAGGTAACTTGGTT+TGG - chr3_4:58002097-58002116 Msa0509570:intron 45.0%
TTGTTATGGCCTGTCATAGG+TGG - chr3_4:58002458-58002477 Msa0509570:CDS 45.0%
GAGCCAGGTAAAGAAATTGG+AGG - chr3_4:58002527-58002546 Msa0509570:intron 45.0%
GAAATTGGAGGTCTCTATCC+TGG - chr3_4:58002539-58002558 Msa0509570:intron 45.0%
AGTGCTTGACAATAGGAGTG+AGG - chr3_4:58003047-58003066 Msa0509570:CDS 45.0%
!!! GCAGAAGAACTTCTGGCTTT+TGG - chr3_4:58003114-58003133 Msa0509570:CDS 45.0%
GATCTCAAGAGGGGAAAATG+GGG - chr3_4:58003172-58003191 Msa0509570:CDS 45.0%
AGTGTAGCAGAGGTTCAGTT+TGG + chr3_4:58001604-58001623 None:intergenic 45.0%
! TAGCTGAAAGAGGGAGTGTA+GGG + chr3_4:58001633-58001652 None:intergenic 45.0%
! ATAGCTGAAAGAGGGAGTGT+AGG + chr3_4:58001634-58001653 None:intergenic 45.0%
GGGACCCAAGATGATTAACA+AGG + chr3_4:58002063-58002082 None:intergenic 45.0%
! CTGGCTTGCAATCTTTGCAA+AGG + chr3_4:58002084-58002103 None:intergenic 45.0%
TTGCAAAGATTGCAAGCCAG+AGG - chr3_4:58002084-58002103 Msa0509570:intron 45.0%
ATTGCAAGCCAGAGGTAACT+TGG - chr3_4:58002092-58002111 Msa0509570:intron 45.0%
AAGCCAGAGGTAACTTGGTT+TGG - chr3_4:58002097-58002116 Msa0509570:intron 45.0%
TTGTTATGGCCTGTCATAGG+TGG - chr3_4:58002458-58002477 Msa0509570:CDS 45.0%
GAGCCAGGTAAAGAAATTGG+AGG - chr3_4:58002527-58002546 Msa0509570:intron 45.0%
GAAATTGGAGGTCTCTATCC+TGG - chr3_4:58002539-58002558 Msa0509570:intron 45.0%
AGTGCTTGACAATAGGAGTG+AGG - chr3_4:58003047-58003066 Msa0509570:CDS 45.0%
!!! GCAGAAGAACTTCTGGCTTT+TGG - chr3_4:58003114-58003133 Msa0509570:CDS 45.0%
GATCTCAAGAGGGGAAAATG+GGG - chr3_4:58003172-58003191 Msa0509570:CDS 45.0%
AGAGAGAGGACATGTGAGAG+GGG + chr3_4:58001521-58001540 None:intergenic 50.0%
GAGAGAGAGGACATGTGAGA+GGG + chr3_4:58001522-58001541 None:intergenic 50.0%
AGAGAGAGAGGACATGTGAG+AGG + chr3_4:58001523-58001542 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG + chr3_4:58001533-58001552 None:intergenic 50.0%
CTTCCACTTCCACCTATGAC+AGG + chr3_4:58002470-58002489 None:intergenic 50.0%
CTCATCACTGTGGCCAGTTT+AGG + chr3_4:58002510-58002529 None:intergenic 50.0%
CTTTACCTGGCTCATCACTG+TGG + chr3_4:58002520-58002539 None:intergenic 50.0%
GAGGTCTCTATCCTGGTACT+TGG - chr3_4:58002546-58002565 Msa0509570:intron 50.0%
! GCTTCTTTTGGCCAAGTACC+AGG + chr3_4:58002560-58002579 None:intergenic 50.0%
ATGGCTACCTTATCAGCACC+AGG - chr3_4:58003089-58003108 Msa0509570:CDS 50.0%
AGAGAGAGGACATGTGAGAG+GGG + chr3_4:58001521-58001540 None:intergenic 50.0%
GAGAGAGAGGACATGTGAGA+GGG + chr3_4:58001522-58001541 None:intergenic 50.0%
AGAGAGAGAGGACATGTGAG+AGG + chr3_4:58001523-58001542 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG + chr3_4:58001533-58001552 None:intergenic 50.0%
CTTCCACTTCCACCTATGAC+AGG + chr3_4:58002470-58002489 None:intergenic 50.0%
CTCATCACTGTGGCCAGTTT+AGG + chr3_4:58002510-58002529 None:intergenic 50.0%
CTTTACCTGGCTCATCACTG+TGG + chr3_4:58002520-58002539 None:intergenic 50.0%
GAGGTCTCTATCCTGGTACT+TGG - chr3_4:58002546-58002565 Msa0509570:intron 50.0%
! GCTTCTTTTGGCCAAGTACC+AGG + chr3_4:58002560-58002579 None:intergenic 50.0%
ATGGCTACCTTATCAGCACC+AGG - chr3_4:58003089-58003108 Msa0509570:CDS 50.0%
AGGGAGCTAGAGTGTAGCAG+AGG + chr3_4:58001614-58001633 None:intergenic 55.0%
TGGCCTGTCATAGGTGGAAG+TGG - chr3_4:58002464-58002483 Msa0509570:intron 55.0%
CAGCAGCACAGAGGAGTTTG+TGG - chr3_4:58003003-58003022 Msa0509570:CDS 55.0%
GGAGTGAGGAAGAGAAGCCA+AGG - chr3_4:58003061-58003080 Msa0509570:CDS 55.0%
GCTACCTTATCAGCACCAGG+TGG - chr3_4:58003092-58003111 Msa0509570:CDS 55.0%
!! TCTGCCACCTGGTGCTGATA+AGG + chr3_4:58003099-58003118 None:intergenic 55.0%
CCAGAAGTTCTTCTGCCACC+TGG + chr3_4:58003110-58003129 None:intergenic 55.0%
CCAGGTGGCAGAAGAACTTC+TGG - chr3_4:58003107-58003126 Msa0509570:CDS 55.0%
AGGGAGCTAGAGTGTAGCAG+AGG + chr3_4:58001614-58001633 None:intergenic 55.0%
TGGCCTGTCATAGGTGGAAG+TGG - chr3_4:58002464-58002483 Msa0509570:intron 55.0%
CAGCAGCACAGAGGAGTTTG+TGG - chr3_4:58003003-58003022 Msa0509570:CDS 55.0%
GGAGTGAGGAAGAGAAGCCA+AGG - chr3_4:58003061-58003080 Msa0509570:CDS 55.0%
GCTACCTTATCAGCACCAGG+TGG - chr3_4:58003092-58003111 Msa0509570:CDS 55.0%
!! TCTGCCACCTGGTGCTGATA+AGG + chr3_4:58003099-58003118 None:intergenic 55.0%
CCAGAAGTTCTTCTGCCACC+TGG + chr3_4:58003110-58003129 None:intergenic 55.0%
CCAGGTGGCAGAAGAACTTC+TGG - chr3_4:58003107-58003126 Msa0509570:CDS 55.0%
ACTGGCCACAGTGATGAGCC+AGG - chr3_4:58002512-58002531 Msa0509570:intron 60.0%
ACTGGCCACAGTGATGAGCC+AGG - chr3_4:58002512-58002531 Msa0509570:intron 60.0%
CAGCAGCTGCAGCAGCACAG+AGG - chr3_4:58002994-58003013 Msa0509570:CDS 65.0%
CAGCAGCTGCAGCAGCACAG+AGG - chr3_4:58002994-58003013 Msa0509570:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 58001461 58003493 58001461 ID=Msa0509570;Name=Msa0509570
chr3_4 mRNA 58001461 58003493 58001461 ID=Msa0509570-mRNA-1;Parent=Msa0509570;Name=Msa0509570-mRNA-1;_AED=0.04;_eAED=0.04;_QI=255|1|1|1|1|1|3|352|224
chr3_4 exon 58001461 58001975 58001461 ID=Msa0509570-mRNA-1:exon:13408;Parent=Msa0509570-mRNA-1
chr3_4 exon 58002356 58002477 58002356 ID=Msa0509570-mRNA-1:exon:13407;Parent=Msa0509570-mRNA-1
chr3_4 exon 58002849 58003493 58002849 ID=Msa0509570-mRNA-1:exon:13406;Parent=Msa0509570-mRNA-1
chr3_4 five_prime_UTR 58003239 58003493 58003239 ID=Msa0509570-mRNA-1:five_prime_utr;Parent=Msa0509570-mRNA-1
chr3_4 CDS 58002849 58003238 58002849 ID=Msa0509570-mRNA-1:cds;Parent=Msa0509570-mRNA-1
chr3_4 CDS 58002356 58002477 58002356 ID=Msa0509570-mRNA-1:cds;Parent=Msa0509570-mRNA-1
chr3_4 CDS 58001813 58001975 58001813 ID=Msa0509570-mRNA-1:cds;Parent=Msa0509570-mRNA-1
chr3_4 three_prime_UTR 58001461 58001812 58001461 ID=Msa0509570-mRNA-1:three_prime_utr;Parent=Msa0509570-mRNA-1
Gene Sequence

>Msa0509570

ATGCCAAGTTCAGCTTCATTTTTGAGGCAGTTAAGTTCAAAGGAGACTTGGAAATCAACATCAAATAGGTGGAGTGGAAAAAACACATACAACAACAATAGTTTAGGAGTTACTTGTGAGACAAATCTGAGTCAAATGGAAGGGTTTAACATTAACATGTATGGAAATGAAGAGAGTTGTGGAATGATGGTGAGAAAGAGAGTTATGGTAGTGGTTGATGGTACTTCACATTCTAAGCATGCAATGATTTGGGCACTTACTCATGTTGTTAATAAGGGTGATTTGTTGACTTTGCTTTACATTGTTTCTCCTCAAAGTGCTTCTGAGTCTTATTCTTCTACTTACCTTGTTAATCATCTTGGGTCCCTTTGCAAAGATTGCAAGCCAGAGGTGGAAGTGGAAGCACTTGTAATTCAAGGACCTAAACTGGCCACAGTGATGAGCCAGGTAAAGAAATTGGAGGTCTCTATCCTGGTACTTGGCCAAAAGAAGCCATCTTCTTTCTTTAGCTGTCTCTGTGGAAGTAGCAGCAGCTGCAGCAGCACAGAGGAGTTTGTGGAGTACTGTATCAACAATGCTGAGTGCTTGACAATAGGAGTGAGGAAGAGAAGCCAAGGCAATAATGGCTACCTTATCAGCACCAGGTGGCAGAAGAACTTCTGGCTTTTGGCTTAG
Protein sequence

>Msa0509570

MPSSASFLRQLSSKETWKSTSNRWSGKNTYNNNSLGVTCETNLSQMEGFNINMYGNEESCGMMVRKRVMVVVDGTSHSKHAMIWALTHVVNKGDLLTLLYIVSPQSASESYSSTYLVNHLGSLCKDCKPEVEVEALVIQGPKLATVMSQVKKLEVSILVLGQKKPSSFFSCLCGSSSSCSSTEEFVEYCINNAECLTIGVRKRSQGNNGYLISTRWQKNFWLLA*