AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0464580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464580 MtrunA17_Chr3g0115881 100.000 74 0 0 1 74 1 74 2.59e-49 149
Msa0464580 MtrunA17_Chr7g0214091 89.130 46 5 0 1 46 1 46 4.54e-24 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464580 AT1G27435.1 72.000 75 17 1 3 73 6 80 8.74e-33 108

Find 35 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAGATGGGTGGTGCATTT+TGG 0.153987 3_3:+64636018 Msa0464580:intron
AGGTTGTTCCATCGGTATTT+TGG 0.238148 3_3:+64636812 Msa0464580:three_prime_UTR
CACACGGCGAAGACGCTTCT+TGG 0.328655 3_3:-64636132 None:intergenic
GTTGACCGGCTTCATAAAAT+AGG 0.333484 3_3:-64637085 None:intergenic
TGTAAGAAAGTTAAAATCAT+TGG 0.339565 3_3:-64636898 None:intergenic
GTGAAGAAGCATGATTCTTC+GGG 0.371640 3_3:+64636062 Msa0464580:CDS
AGTACTCTTTGTAACATTGT+TGG 0.406629 3_3:-64636766 None:intergenic
AGTGAAGAAGCATGATTCTT+CGG 0.422338 3_3:+64636061 Msa0464580:CDS
ATACCGATGGAACAACCTTC+AGG 0.424726 3_3:-64636807 None:intergenic
GCGAAGACGCTTCTTGGCAT+TGG 0.430692 3_3:-64636126 None:intergenic
ACTTGCTGAAGGAGCTGGTT+CGG 0.434113 3_3:-64636730 None:intergenic
GCGTCTTCGCCGTGTGTGGC+AGG 0.469382 3_3:+64636139 Msa0464580:CDS
ACAAAGAGTACTTAAGTTGA+AGG 0.470492 3_3:+64636777 Msa0464580:exon
GGCATTGGCTTTACGAGTTG+TGG 0.490914 3_3:-64636111 None:intergenic
CTTTCTTCGTCTCTTCCCAC+AGG 0.499169 3_3:+64635573 Msa0464580:five_prime_UTR
CTTTGTAACATTGTTGGCAC+TGG 0.504174 3_3:-64636760 None:intergenic
GAAGAACTTGCTGAAGGAGC+TGG 0.514090 3_3:-64636735 None:intergenic
CACATTATAGAACTGTTGAC+CGG 0.525045 3_3:-64637099 None:intergenic
TTGGGGTACGCGGGTGATGG+AGG 0.527082 3_3:+64636037 Msa0464580:CDS
CCACCTGAAGGTTGTTCCAT+CGG 0.559409 3_3:+64636804 Msa0464580:three_prime_UTR
GAAGAATGAAGCTGTCCTCA+AGG 0.567341 3_3:+64636701 Msa0464580:intron
CAAAGAGTACTTAAGTTGAA+GGG 0.582974 3_3:+64636778 Msa0464580:exon
GTTGAAGGGTGTCCACCTGA+AGG 0.585228 3_3:+64636792 Msa0464580:three_prime_UTR
GATGTGAATCGCTCAATCGC+GGG 0.589266 3_3:-64635540 None:intergenic
TGATGTGAATCGCTCAATCG+CGG 0.592200 3_3:-64635541 None:intergenic
AGAAGCGTCTTCGCCGTGTG+TGG 0.603774 3_3:+64636135 Msa0464580:CDS
GTGAATCGCTCAATCGCGGG+AGG 0.610710 3_3:-64635537 None:intergenic
TTGGGATCCCAAAATACCGA+TGG 0.617975 3_3:-64636820 None:intergenic
CTGGCAGAAGAACTTGCTGA+AGG 0.618337 3_3:-64636741 None:intergenic
ATTGAAATACCTGCCACACA+CGG 0.634795 3_3:-64636148 None:intergenic
TGAATCGCTCAATCGCGGGA+GGG 0.673908 3_3:-64635536 None:intergenic
TGAAGAAGCATGATTCTTCG+GGG 0.674050 3_3:+64636063 Msa0464580:CDS
CCGATGGAACAACCTTCAGG+TGG 0.677190 3_3:-64636804 None:intergenic
CTGGTTCGGAACAAGCCTTG+AGG 0.710113 3_3:-64636716 None:intergenic
GGTAAAATAAAATAGATGGG+TGG 0.724755 3_3:+64636008 Msa0464580:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGAAATAAAAAAGTAAATTA+AGG - chr3_3:64635643-64635662 None:intergenic 15.0%
!!! ACATTTTTCAGATAAATTAA+AGG - chr3_3:64635664-64635683 None:intergenic 15.0%
!! AAGCATGTTATTAATTAAAT+AGG + chr3_3:64636221-64636240 Msa0464580:intron 15.0%
!! GGAAATAAAAAAGTAAATTA+AGG - chr3_3:64635643-64635662 None:intergenic 15.0%
!!! ACATTTTTCAGATAAATTAA+AGG - chr3_3:64635664-64635683 None:intergenic 15.0%
!! AAGCATGTTATTAATTAAAT+AGG + chr3_3:64636221-64636240 Msa0464580:intron 15.0%
!!! AAGTTTTAATATATGTGTAG+CGG + chr3_3:64635746-64635765 Msa0464580:intron 20.0%
!!! AAACTATAAGTGAAACTTTT+AGG + chr3_3:64636438-64636457 Msa0464580:intron 20.0%
!! CACAATTATTGTTCAATATT+TGG - chr3_3:64636641-64636660 None:intergenic 20.0%
!!! TATGGTACATTTTTTGTTTT+TGG + chr3_3:64636679-64636698 Msa0464580:intron 20.0%
!! GCAAAAACTAATATAGAATT+GGG - chr3_3:64636841-64636860 None:intergenic 20.0%
!! TGTAAGAAAGTTAAAATCAT+TGG - chr3_3:64636901-64636920 None:intergenic 20.0%
!!! TGTAAGTTGTAAACTTTTTT+AGG + chr3_3:64636931-64636950 Msa0464580:three_prime_UTR 20.0%
!!! GTAAGTTGTAAACTTTTTTA+GGG + chr3_3:64636932-64636951 Msa0464580:three_prime_UTR 20.0%
!!! AAGTTTTAATATATGTGTAG+CGG + chr3_3:64635746-64635765 Msa0464580:intron 20.0%
!!! AAACTATAAGTGAAACTTTT+AGG + chr3_3:64636438-64636457 Msa0464580:intron 20.0%
!! CACAATTATTGTTCAATATT+TGG - chr3_3:64636641-64636660 None:intergenic 20.0%
!!! TATGGTACATTTTTTGTTTT+TGG + chr3_3:64636679-64636698 Msa0464580:intron 20.0%
!! GCAAAAACTAATATAGAATT+GGG - chr3_3:64636841-64636860 None:intergenic 20.0%
!! TGTAAGAAAGTTAAAATCAT+TGG - chr3_3:64636901-64636920 None:intergenic 20.0%
!!! TGTAAGTTGTAAACTTTTTT+AGG + chr3_3:64636931-64636950 Msa0464580:three_prime_UTR 20.0%
!!! GTAAGTTGTAAACTTTTTTA+GGG + chr3_3:64636932-64636951 Msa0464580:three_prime_UTR 20.0%
! GATTGTGATTTATGGTATTA+TGG + chr3_3:64635688-64635707 Msa0464580:intron 25.0%
!!! TCCTAAGTTTTTTACATTAG+CGG + chr3_3:64635775-64635794 Msa0464580:intron 25.0%
! TAGTAATAATCTATGAAGCA+CGG - chr3_3:64635844-64635863 None:intergenic 25.0%
! TGCTGGTAAAATAAAATAGA+TGG + chr3_3:64636004-64636023 Msa0464580:intron 25.0%
! GCTGGTAAAATAAAATAGAT+GGG + chr3_3:64636005-64636024 Msa0464580:intron 25.0%
! ATACCTAGAAGAAATGATTA+AGG + chr3_3:64636250-64636269 Msa0464580:intron 25.0%
!!! ATTTATTGTTTTATCGTCTG+TGG + chr3_3:64636279-64636298 Msa0464580:intron 25.0%
!! GTTTCACTTATAGTTTTTCT+AGG - chr3_3:64636434-64636453 None:intergenic 25.0%
!!! TGAAACTTTTAGGAAAAACT+TGG + chr3_3:64636448-64636467 Msa0464580:intron 25.0%
! TATGGTTTGAGTAAAGATTA+TGG + chr3_3:64636493-64636512 Msa0464580:intron 25.0%
! GGCAAAAACTAATATAGAAT+TGG - chr3_3:64636842-64636861 None:intergenic 25.0%
! GATTGTGATTTATGGTATTA+TGG + chr3_3:64635688-64635707 Msa0464580:intron 25.0%
!!! TCCTAAGTTTTTTACATTAG+CGG + chr3_3:64635775-64635794 Msa0464580:intron 25.0%
! TAGTAATAATCTATGAAGCA+CGG - chr3_3:64635844-64635863 None:intergenic 25.0%
! TGCTGGTAAAATAAAATAGA+TGG + chr3_3:64636004-64636023 Msa0464580:intron 25.0%
! GCTGGTAAAATAAAATAGAT+GGG + chr3_3:64636005-64636024 Msa0464580:intron 25.0%
! ATACCTAGAAGAAATGATTA+AGG + chr3_3:64636250-64636269 Msa0464580:intron 25.0%
!!! ATTTATTGTTTTATCGTCTG+TGG + chr3_3:64636279-64636298 Msa0464580:intron 25.0%
!! GTTTCACTTATAGTTTTTCT+AGG - chr3_3:64636434-64636453 None:intergenic 25.0%
!!! TGAAACTTTTAGGAAAAACT+TGG + chr3_3:64636448-64636467 Msa0464580:intron 25.0%
! TATGGTTTGAGTAAAGATTA+TGG + chr3_3:64636493-64636512 Msa0464580:intron 25.0%
! GGCAAAAACTAATATAGAAT+TGG - chr3_3:64636842-64636861 None:intergenic 25.0%
ATGTTGCAGATTGTGATTTA+TGG + chr3_3:64635680-64635699 Msa0464580:intron 30.0%
ACCGCTAATGTAAAAAACTT+AGG - chr3_3:64635779-64635798 None:intergenic 30.0%
!!! TTGAATCTGATTTGTTTTGC+TGG + chr3_3:64635987-64636006 Msa0464580:intron 30.0%
GGTAAAATAAAATAGATGGG+TGG + chr3_3:64636008-64636027 Msa0464580:intron 30.0%
CATCCTTAATCATTTCTTCT+AGG - chr3_3:64636256-64636275 None:intergenic 30.0%
TGATGAGCATTTAGTAAAGA+TGG + chr3_3:64636308-64636327 Msa0464580:intron 30.0%
TCTACTAGTACAAAATCTAC+AGG - chr3_3:64636371-64636390 None:intergenic 30.0%
! TTTTGTACTAGTAGATCAGA+TGG + chr3_3:64636376-64636395 Msa0464580:intron 30.0%
AAACTTGGAGATTAATGAGT+TGG + chr3_3:64636463-64636482 Msa0464580:intron 30.0%
TCTTTACTCAAACCATATCT+TGG - chr3_3:64636490-64636509 None:intergenic 30.0%
ACAATAATTGTGACTGTCAA+TGG + chr3_3:64636649-64636668 Msa0464580:intron 30.0%
AGTACTCTTTGTAACATTGT+TGG - chr3_3:64636769-64636788 None:intergenic 30.0%
!! ACAAAGAGTACTTAAGTTGA+AGG + chr3_3:64636777-64636796 Msa0464580:exon 30.0%
!! CAAAGAGTACTTAAGTTGAA+GGG + chr3_3:64636778-64636797 Msa0464580:exon 30.0%
!! TCTACAGTTTTTCAAATGTG+TGG - chr3_3:64636863-64636882 None:intergenic 30.0%
ACACATTTGAAAAACTGTAG+AGG + chr3_3:64636862-64636881 Msa0464580:three_prime_UTR 30.0%
ATGTTGCAGATTGTGATTTA+TGG + chr3_3:64635680-64635699 Msa0464580:intron 30.0%
ACCGCTAATGTAAAAAACTT+AGG - chr3_3:64635779-64635798 None:intergenic 30.0%
!!! TTGAATCTGATTTGTTTTGC+TGG + chr3_3:64635987-64636006 Msa0464580:intron 30.0%
GGTAAAATAAAATAGATGGG+TGG + chr3_3:64636008-64636027 Msa0464580:intron 30.0%
CATCCTTAATCATTTCTTCT+AGG - chr3_3:64636256-64636275 None:intergenic 30.0%
TGATGAGCATTTAGTAAAGA+TGG + chr3_3:64636308-64636327 Msa0464580:intron 30.0%
TCTACTAGTACAAAATCTAC+AGG - chr3_3:64636371-64636390 None:intergenic 30.0%
! TTTTGTACTAGTAGATCAGA+TGG + chr3_3:64636376-64636395 Msa0464580:intron 30.0%
AAACTTGGAGATTAATGAGT+TGG + chr3_3:64636463-64636482 Msa0464580:intron 30.0%
TCTTTACTCAAACCATATCT+TGG - chr3_3:64636490-64636509 None:intergenic 30.0%
ACAATAATTGTGACTGTCAA+TGG + chr3_3:64636649-64636668 Msa0464580:intron 30.0%
AGTACTCTTTGTAACATTGT+TGG - chr3_3:64636769-64636788 None:intergenic 30.0%
!! ACAAAGAGTACTTAAGTTGA+AGG + chr3_3:64636777-64636796 Msa0464580:exon 30.0%
!! CAAAGAGTACTTAAGTTGAA+GGG + chr3_3:64636778-64636797 Msa0464580:exon 30.0%
!! TCTACAGTTTTTCAAATGTG+TGG - chr3_3:64636863-64636882 None:intergenic 30.0%
ACACATTTGAAAAACTGTAG+AGG + chr3_3:64636862-64636881 Msa0464580:three_prime_UTR 30.0%
ACAAATTGCTTCTGACTACT+GGG + chr3_3:64635937-64635956 Msa0464580:intron 35.0%
! AGTGAAGAAGCATGATTCTT+CGG + chr3_3:64636061-64636080 Msa0464580:CDS 35.0%
TGAGCATTTAGTAAAGATGG+TGG + chr3_3:64636311-64636330 Msa0464580:intron 35.0%
!! AAGATGGTGGAAAATAAGCT+TGG + chr3_3:64636324-64636343 Msa0464580:intron 35.0%
GTGGAAAATAAGCTTGGTTT+GGG + chr3_3:64636330-64636349 Msa0464580:intron 35.0%
! TAATGAGTTGGACCAAGATA+TGG + chr3_3:64636475-64636494 Msa0464580:intron 35.0%
ACTGTCAATGGCTAATGTTA+TGG + chr3_3:64636661-64636680 Msa0464580:intron 35.0%
! TCTTGCCTATTTTATGAAGC+CGG + chr3_3:64637080-64637099 Msa0464580:three_prime_UTR 35.0%
ACAAATTGCTTCTGACTACT+GGG + chr3_3:64635937-64635956 Msa0464580:intron 35.0%
! AGTGAAGAAGCATGATTCTT+CGG + chr3_3:64636061-64636080 Msa0464580:CDS 35.0%
TGAGCATTTAGTAAAGATGG+TGG + chr3_3:64636311-64636330 Msa0464580:intron 35.0%
!! AAGATGGTGGAAAATAAGCT+TGG + chr3_3:64636324-64636343 Msa0464580:intron 35.0%
GTGGAAAATAAGCTTGGTTT+GGG + chr3_3:64636330-64636349 Msa0464580:intron 35.0%
! TAATGAGTTGGACCAAGATA+TGG + chr3_3:64636475-64636494 Msa0464580:intron 35.0%
ACTGTCAATGGCTAATGTTA+TGG + chr3_3:64636661-64636680 Msa0464580:intron 35.0%
! TCTTGCCTATTTTATGAAGC+CGG + chr3_3:64637080-64637099 Msa0464580:three_prime_UTR 35.0%
!!! GTTTTAGGGTTTAGTTGAGG+AGG - chr3_3:64635517-64635536 None:intergenic 40.0%
!!! AGGGTTTTAGGGTTTAGTTG+AGG - chr3_3:64635520-64635539 None:intergenic 40.0%
GAGAGAAGAAAGAAACCTGT+GGG - chr3_3:64635591-64635610 None:intergenic 40.0%
AGAGAGAAGAAAGAAACCTG+TGG - chr3_3:64635592-64635611 None:intergenic 40.0%
!! TTTTTACATTAGCGGTGTCG+AGG + chr3_3:64635783-64635802 Msa0464580:intron 40.0%
GAAGCAATTTGTGATCAAGC+TGG - chr3_3:64635929-64635948 None:intergenic 40.0%
CACAAATTGCTTCTGACTAC+TGG + chr3_3:64635936-64635955 Msa0464580:intron 40.0%
! AATAGATGGGTGGTGCATTT+TGG + chr3_3:64636018-64636037 Msa0464580:intron 40.0%
! ATAGATGGGTGGTGCATTTT+GGG + chr3_3:64636019-64636038 Msa0464580:intron 40.0%
! GTGAAGAAGCATGATTCTTC+GGG + chr3_3:64636062-64636081 Msa0464580:CDS 40.0%
! TGAAGAAGCATGATTCTTCG+GGG + chr3_3:64636063-64636082 Msa0464580:CDS 40.0%
! ATGATTCTTCGGGGCTTTTA+TGG + chr3_3:64636072-64636091 Msa0464580:CDS 40.0%
ATTGAAATACCTGCCACACA+CGG - chr3_3:64636151-64636170 None:intergenic 40.0%
GGTGGAAAATAAGCTTGGTT+TGG + chr3_3:64636329-64636348 Msa0464580:intron 40.0%
CTAGTAGATCAGATGGAGAA+TGG + chr3_3:64636383-64636402 Msa0464580:intron 40.0%
TAGATCAGATGGAGAATGGT+CGG + chr3_3:64636387-64636406 Msa0464580:intron 40.0%
CTTTGTAACATTGTTGGCAC+TGG - chr3_3:64636763-64636782 None:intergenic 40.0%
! AGGTTGTTCCATCGGTATTT+TGG + chr3_3:64636812-64636831 Msa0464580:three_prime_UTR 40.0%
! GGTTGTTCCATCGGTATTTT+GGG + chr3_3:64636813-64636832 Msa0464580:three_prime_UTR 40.0%
! GTTGACCGGCTTCATAAAAT+AGG - chr3_3:64637088-64637107 None:intergenic 40.0%
!!! GTTTTAGGGTTTAGTTGAGG+AGG - chr3_3:64635517-64635536 None:intergenic 40.0%
!!! AGGGTTTTAGGGTTTAGTTG+AGG - chr3_3:64635520-64635539 None:intergenic 40.0%
GAGAGAAGAAAGAAACCTGT+GGG - chr3_3:64635591-64635610 None:intergenic 40.0%
AGAGAGAAGAAAGAAACCTG+TGG - chr3_3:64635592-64635611 None:intergenic 40.0%
!! TTTTTACATTAGCGGTGTCG+AGG + chr3_3:64635783-64635802 Msa0464580:intron 40.0%
GAAGCAATTTGTGATCAAGC+TGG - chr3_3:64635929-64635948 None:intergenic 40.0%
CACAAATTGCTTCTGACTAC+TGG + chr3_3:64635936-64635955 Msa0464580:intron 40.0%
! AATAGATGGGTGGTGCATTT+TGG + chr3_3:64636018-64636037 Msa0464580:intron 40.0%
! ATAGATGGGTGGTGCATTTT+GGG + chr3_3:64636019-64636038 Msa0464580:intron 40.0%
! GTGAAGAAGCATGATTCTTC+GGG + chr3_3:64636062-64636081 Msa0464580:CDS 40.0%
! TGAAGAAGCATGATTCTTCG+GGG + chr3_3:64636063-64636082 Msa0464580:CDS 40.0%
! ATGATTCTTCGGGGCTTTTA+TGG + chr3_3:64636072-64636091 Msa0464580:CDS 40.0%
ATTGAAATACCTGCCACACA+CGG - chr3_3:64636151-64636170 None:intergenic 40.0%
GGTGGAAAATAAGCTTGGTT+TGG + chr3_3:64636329-64636348 Msa0464580:intron 40.0%
CTAGTAGATCAGATGGAGAA+TGG + chr3_3:64636383-64636402 Msa0464580:intron 40.0%
TAGATCAGATGGAGAATGGT+CGG + chr3_3:64636387-64636406 Msa0464580:intron 40.0%
CTTTGTAACATTGTTGGCAC+TGG - chr3_3:64636763-64636782 None:intergenic 40.0%
! AGGTTGTTCCATCGGTATTT+TGG + chr3_3:64636812-64636831 Msa0464580:three_prime_UTR 40.0%
! GGTTGTTCCATCGGTATTTT+GGG + chr3_3:64636813-64636832 Msa0464580:three_prime_UTR 40.0%
! GTTGACCGGCTTCATAAAAT+AGG - chr3_3:64637088-64637107 None:intergenic 40.0%
TGATGTGAATCGCTCAATCG+CGG - chr3_3:64635544-64635563 None:intergenic 45.0%
GCAATTTGTGATCAAGCTGG+CGG - chr3_3:64635926-64635945 None:intergenic 45.0%
! TAGATGGGTGGTGCATTTTG+GGG + chr3_3:64636020-64636039 Msa0464580:intron 45.0%
AAGCCCTATCTCACTAAGTG+GGG - chr3_3:64636542-64636561 None:intergenic 45.0%
CAAGCCCTATCTCACTAAGT+GGG - chr3_3:64636543-64636562 None:intergenic 45.0%
GAAGAATGAAGCTGTCCTCA+AGG + chr3_3:64636701-64636720 Msa0464580:intron 45.0%
ATACCGATGGAACAACCTTC+AGG - chr3_3:64636810-64636829 None:intergenic 45.0%
TTGGGATCCCAAAATACCGA+TGG - chr3_3:64636823-64636842 None:intergenic 45.0%
TGATGTGAATCGCTCAATCG+CGG - chr3_3:64635544-64635563 None:intergenic 45.0%
GCAATTTGTGATCAAGCTGG+CGG - chr3_3:64635926-64635945 None:intergenic 45.0%
! TAGATGGGTGGTGCATTTTG+GGG + chr3_3:64636020-64636039 Msa0464580:intron 45.0%
AAGCCCTATCTCACTAAGTG+GGG - chr3_3:64636542-64636561 None:intergenic 45.0%
CAAGCCCTATCTCACTAAGT+GGG - chr3_3:64636543-64636562 None:intergenic 45.0%
GAAGAATGAAGCTGTCCTCA+AGG + chr3_3:64636701-64636720 Msa0464580:intron 45.0%
ATACCGATGGAACAACCTTC+AGG - chr3_3:64636810-64636829 None:intergenic 45.0%
TTGGGATCCCAAAATACCGA+TGG - chr3_3:64636823-64636842 None:intergenic 45.0%
! TCAATCGCGGGAGGGTTTTA+GGG - chr3_3:64635531-64635550 None:intergenic 50.0%
GATGTGAATCGCTCAATCGC+GGG - chr3_3:64635543-64635562 None:intergenic 50.0%
CTTTCTTCGTCTCTTCCCAC+AGG + chr3_3:64635573-64635592 Msa0464580:five_prime_UTR 50.0%
ATGAAGCACGGACTCGAACA+CGG - chr3_3:64635832-64635851 None:intergenic 50.0%
!! GGCATTGGCTTTACGAGTTG+TGG - chr3_3:64636114-64636133 None:intergenic 50.0%
GGTTTGGGCATGTAGAGTGA+AGG + chr3_3:64636345-64636364 Msa0464580:intron 50.0%
GAGAATGGTCGGATCACTAG+AGG + chr3_3:64636398-64636417 Msa0464580:intron 50.0%
CCTATCTCACTAAGTGGGGT+CGG - chr3_3:64636538-64636557 None:intergenic 50.0%
CGACCCCACTTAGTGAGATA+GGG + chr3_3:64636536-64636555 Msa0464580:intron 50.0%
CCAAGCCCTATCTCACTAAG+TGG - chr3_3:64636544-64636563 None:intergenic 50.0%
CCACTTAGTGAGATAGGGCT+TGG + chr3_3:64636541-64636560 Msa0464580:intron 50.0%
! ACTTGCTGAAGGAGCTGGTT+CGG - chr3_3:64636733-64636752 None:intergenic 50.0%
!! GAAGAACTTGCTGAAGGAGC+TGG - chr3_3:64636738-64636757 None:intergenic 50.0%
!! CTGGCAGAAGAACTTGCTGA+AGG - chr3_3:64636744-64636763 None:intergenic 50.0%
CCACCTGAAGGTTGTTCCAT+CGG + chr3_3:64636804-64636823 Msa0464580:three_prime_UTR 50.0%
! TCAATCGCGGGAGGGTTTTA+GGG - chr3_3:64635531-64635550 None:intergenic 50.0%
GATGTGAATCGCTCAATCGC+GGG - chr3_3:64635543-64635562 None:intergenic 50.0%
CTTTCTTCGTCTCTTCCCAC+AGG + chr3_3:64635573-64635592 Msa0464580:five_prime_UTR 50.0%
ATGAAGCACGGACTCGAACA+CGG - chr3_3:64635832-64635851 None:intergenic 50.0%
!! GGCATTGGCTTTACGAGTTG+TGG - chr3_3:64636114-64636133 None:intergenic 50.0%
GGTTTGGGCATGTAGAGTGA+AGG + chr3_3:64636345-64636364 Msa0464580:intron 50.0%
GAGAATGGTCGGATCACTAG+AGG + chr3_3:64636398-64636417 Msa0464580:intron 50.0%
CCTATCTCACTAAGTGGGGT+CGG - chr3_3:64636538-64636557 None:intergenic 50.0%
CGACCCCACTTAGTGAGATA+GGG + chr3_3:64636536-64636555 Msa0464580:intron 50.0%
CCAAGCCCTATCTCACTAAG+TGG - chr3_3:64636544-64636563 None:intergenic 50.0%
CCACTTAGTGAGATAGGGCT+TGG + chr3_3:64636541-64636560 Msa0464580:intron 50.0%
! ACTTGCTGAAGGAGCTGGTT+CGG - chr3_3:64636733-64636752 None:intergenic 50.0%
!! GAAGAACTTGCTGAAGGAGC+TGG - chr3_3:64636738-64636757 None:intergenic 50.0%
!! CTGGCAGAAGAACTTGCTGA+AGG - chr3_3:64636744-64636763 None:intergenic 50.0%
CCACCTGAAGGTTGTTCCAT+CGG + chr3_3:64636804-64636823 Msa0464580:three_prime_UTR 50.0%
! CTCAATCGCGGGAGGGTTTT+AGG - chr3_3:64635532-64635551 None:intergenic 55.0%
TGAATCGCTCAATCGCGGGA+GGG - chr3_3:64635539-64635558 None:intergenic 55.0%
AGGTGTCTGTGTGTCGTGTC+AGG + chr3_3:64635803-64635822 Msa0464580:intron 55.0%
!!! GTGGTGCATTTTGGGGTACG+CGG + chr3_3:64636027-64636046 Msa0464580:CDS 55.0%
!!! TGGTGCATTTTGGGGTACGC+GGG + chr3_3:64636028-64636047 Msa0464580:CDS 55.0%
!!! ATTTTGGGGTACGCGGGTGA+TGG + chr3_3:64636034-64636053 Msa0464580:CDS 55.0%
GCGAAGACGCTTCTTGGCAT+TGG - chr3_3:64636129-64636148 None:intergenic 55.0%
GGTCGGATCACTAGAGGAAG+AGG + chr3_3:64636404-64636423 Msa0464580:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG - chr3_3:64636530-64636549 None:intergenic 55.0%
CCGACCCCACTTAGTGAGAT+AGG + chr3_3:64636535-64636554 Msa0464580:intron 55.0%
!! CTGGTTCGGAACAAGCCTTG+AGG - chr3_3:64636719-64636738 None:intergenic 55.0%
!! GTTGAAGGGTGTCCACCTGA+AGG + chr3_3:64636792-64636811 Msa0464580:three_prime_UTR 55.0%
CCGATGGAACAACCTTCAGG+TGG - chr3_3:64636807-64636826 None:intergenic 55.0%
! CTCAATCGCGGGAGGGTTTT+AGG - chr3_3:64635532-64635551 None:intergenic 55.0%
TGAATCGCTCAATCGCGGGA+GGG - chr3_3:64635539-64635558 None:intergenic 55.0%
AGGTGTCTGTGTGTCGTGTC+AGG + chr3_3:64635803-64635822 Msa0464580:intron 55.0%
!!! GTGGTGCATTTTGGGGTACG+CGG + chr3_3:64636027-64636046 Msa0464580:CDS 55.0%
!!! TGGTGCATTTTGGGGTACGC+GGG + chr3_3:64636028-64636047 Msa0464580:CDS 55.0%
!!! ATTTTGGGGTACGCGGGTGA+TGG + chr3_3:64636034-64636053 Msa0464580:CDS 55.0%
GCGAAGACGCTTCTTGGCAT+TGG - chr3_3:64636129-64636148 None:intergenic 55.0%
GGTCGGATCACTAGAGGAAG+AGG + chr3_3:64636404-64636423 Msa0464580:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG - chr3_3:64636530-64636549 None:intergenic 55.0%
CCGACCCCACTTAGTGAGAT+AGG + chr3_3:64636535-64636554 Msa0464580:intron 55.0%
!! CTGGTTCGGAACAAGCCTTG+AGG - chr3_3:64636719-64636738 None:intergenic 55.0%
!! GTTGAAGGGTGTCCACCTGA+AGG + chr3_3:64636792-64636811 Msa0464580:three_prime_UTR 55.0%
CCGATGGAACAACCTTCAGG+TGG - chr3_3:64636807-64636826 None:intergenic 55.0%
GTGAATCGCTCAATCGCGGG+AGG - chr3_3:64635540-64635559 None:intergenic 60.0%
CACACGGCGAAGACGCTTCT+TGG - chr3_3:64636135-64636154 None:intergenic 60.0%
!! AGAAGCGTCTTCGCCGTGTG+TGG + chr3_3:64636135-64636154 Msa0464580:CDS 60.0%
GTGAATCGCTCAATCGCGGG+AGG - chr3_3:64635540-64635559 None:intergenic 60.0%
CACACGGCGAAGACGCTTCT+TGG - chr3_3:64636135-64636154 None:intergenic 60.0%
!! AGAAGCGTCTTCGCCGTGTG+TGG + chr3_3:64636135-64636154 Msa0464580:CDS 60.0%
! TTGGGGTACGCGGGTGATGG+AGG + chr3_3:64636037-64636056 Msa0464580:CDS 65.0%
! TTGGGGTACGCGGGTGATGG+AGG + chr3_3:64636037-64636056 Msa0464580:CDS 65.0%
GCGTCTTCGCCGTGTGTGGC+AGG + chr3_3:64636139-64636158 Msa0464580:CDS 70.0%
GCGTCTTCGCCGTGTGTGGC+AGG + chr3_3:64636139-64636158 Msa0464580:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 64635494 64637119 64635494 ID=Msa0464580;Name=Msa0464580
chr3_3 mRNA 64635494 64637119 64635494 ID=Msa0464580-mRNA-1;Parent=Msa0464580;Name=Msa0464580-mRNA-1;_AED=0.02;_eAED=0.02;_QI=101|1|1|1|0.5|0.66|3|328|74
chr3_3 exon 64635494 64635594 64635494 ID=Msa0464580-mRNA-1:exon:14228;Parent=Msa0464580-mRNA-1
chr3_3 exon 64636023 64636160 64636023 ID=Msa0464580-mRNA-1:exon:14229;Parent=Msa0464580-mRNA-1
chr3_3 exon 64636705 64637119 64636705 ID=Msa0464580-mRNA-1:exon:14230;Parent=Msa0464580-mRNA-1
chr3_3 five_prime_UTR 64635494 64635594 64635494 ID=Msa0464580-mRNA-1:five_prime_utr;Parent=Msa0464580-mRNA-1
chr3_3 CDS 64636023 64636160 64636023 ID=Msa0464580-mRNA-1:cds;Parent=Msa0464580-mRNA-1
chr3_3 CDS 64636705 64636791 64636705 ID=Msa0464580-mRNA-1:cds;Parent=Msa0464580-mRNA-1
chr3_3 three_prime_UTR 64636792 64637119 64636792 ID=Msa0464580-mRNA-1:three_prime_utr;Parent=Msa0464580-mRNA-1
Gene Sequence

>Msa0464580

ATGGGTGGTGCATTTTGGGGTACGCGGGTGATGGAGGTAGTGAAGAAGCATGATTCTTCGGGGCTTTTATGGAAGCGAATCAAACTCACCACAACTCGTAAAGCCAATGCCAAGAAGCGTCTTCGCCGTGTGTGGCAGAATGAAGCTGTCCTCAAGGCTTGTTCCGAACCAGCTCCTTCAGCAAGTTCTTCTGCCAGTGCCAACAATGTTACAAAGAGTACTTAA
Protein sequence

>Msa0464580

MGGAFWGTRVMEVVKKHDSSGLLWKRIKLTTTRKANAKKRLRRVWQNEAVLKACSEPAPSASSSASANNVTKST*