AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0467380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0467380 MtrunA17_Chr3g0121631 94.915 118 4 1 1 118 1 116 1.04e-77 227
Msa0467380 MtrunA17_Chr7g0219021 48.000 125 58 3 1 120 1 123 6.07e-33 116
Msa0467380 MtrunA17_Chr4g0061451 49.057 159 58 6 1 142 1 153 3.61e-32 115
Msa0467380 MtrunA17_Chr3g0086861 45.238 126 58 4 1 117 1 124 1.46e-29 111
Msa0467380 MtrunA17_Chr7g0219031 45.455 110 51 3 1 103 1 108 2.72e-26 95.9
Msa0467380 MtrunA17_Chr3g0119311 46.392 97 49 2 36 131 36 130 2.63e-25 94.0
Msa0467380 MtrunA17_Chr7g0266751 44.340 106 55 2 23 125 19 123 2.65e-23 89.0
Msa0467380 MtrunA17_Chr4g0031091 44.118 102 54 2 15 115 12 111 4.31e-23 87.4
Msa0467380 MtrunA17_Chr4g0035001 51.282 78 35 2 37 113 7 82 7.63e-22 85.9
Msa0467380 MtrunA17_Chr4g0035051 48.101 79 38 2 37 114 9 85 5.14e-21 82.4
Msa0467380 MtrunA17_Chr4g0035031 50.000 78 36 2 37 113 9 84 3.42e-20 82.0
Msa0467380 MtrunA17_Chr2g0278661 49.367 79 37 2 37 114 8 84 9.17e-19 79.7
Msa0467380 MtrunA17_Chr3g0116631 45.882 85 43 2 37 120 207 289 1.39e-18 79.7
Msa0467380 MtrunA17_Chr7g0263001 39.837 123 62 5 1 118 1 116 1.41e-17 74.3
Msa0467380 MtrunA17_Chr3g0101061 40.351 114 58 5 11 117 8 118 1.73e-17 78.2
Msa0467380 MtrunA17_Chr1g0181111 48.780 82 39 2 37 117 197 276 6.63e-17 75.1
Msa0467380 MtrunA17_Chr6g0449481 36.283 113 68 3 31 142 128 237 1.22e-16 75.5
Msa0467380 MtrunA17_Chr4g0042161 43.011 93 48 3 31 120 44 134 6.08e-16 70.1
Msa0467380 MtrunA17_Chr4g0058151 45.679 81 41 2 37 116 217 295 6.51e-16 72.8
Msa0467380 MtrunA17_Chr4g0058151 38.235 102 60 2 37 137 123 222 5.05e-11 58.9
Msa0467380 MtrunA17_Chr4g0050781 40.000 90 50 3 37 125 8 94 9.54e-16 70.9
Msa0467380 MtrunA17_Chr1g0198431 37.113 97 55 3 33 128 189 280 1.24e-15 71.6
Msa0467380 MtrunA17_Chr4g0038111 39.423 104 60 2 30 133 333 433 2.33e-15 72.0
Msa0467380 MtrunA17_Chr1g0168601 46.835 79 39 2 37 114 154 230 1.63e-14 68.2
Msa0467380 MtrunA17_Chr4g0017401 35.354 99 40 2 36 110 16 114 2.99e-14 67.8
Msa0467380 MtrunA17_Chr3g0144311 35.556 90 57 1 36 125 29 117 3.53e-14 67.8
Msa0467380 MtrunA17_Chr7g0276531 40.845 71 40 1 36 106 18 86 4.69e-14 67.0
Msa0467380 MtrunA17_Chr5g0447761 41.463 82 45 1 35 116 5 83 6.84e-14 67.8
Msa0467380 MtrunA17_Chr3g0120721 33.065 124 73 2 33 147 103 225 8.30e-14 67.0
Msa0467380 MtrunA17_Chr8g0346011 41.791 67 39 0 36 102 37 103 8.48e-14 66.6
Msa0467380 MtrunA17_Chr7g0268551 39.286 84 50 1 38 121 350 432 9.27e-14 67.4
Msa0467380 MtrunA17_Chr2g0327921 40.244 82 46 2 36 116 7 86 1.20e-13 62.4
Msa0467380 MtrunA17_Chr1g0210241 36.782 87 52 2 34 119 5 89 1.44e-13 65.9
Msa0467380 MtrunA17_Chr1g0201001 34.043 94 61 1 38 131 31 123 1.49e-13 66.6
Msa0467380 MtrunA17_Chr3g0110211 42.697 89 47 3 36 122 112 198 1.76e-13 66.2
Msa0467380 MtrunA17_Chr1g0186351 35.632 87 54 1 32 118 108 192 2.34e-13 66.2
Msa0467380 MtrunA17_Chr1g0186351 40.000 70 38 1 37 102 7 76 4.19e-12 62.4
Msa0467380 MtrunA17_Chr2g0290191 40.476 84 47 2 33 116 1 81 2.65e-13 65.9
Msa0467380 MtrunA17_Chr3g0127751 38.961 77 47 0 36 112 26 102 5.18e-13 64.3
Msa0467380 MtrunA17_Chr6g0481071 33.333 90 51 3 36 124 95 176 9.04e-13 62.4
Msa0467380 MtrunA17_Chr3g0088511 35.417 96 56 4 18 110 13 105 1.29e-12 61.2
Msa0467380 MtrunA17_Chr5g0426311 37.313 67 42 0 36 102 16 82 1.41e-12 63.2
Msa0467380 MtrunA17_Chr3g0135111 36.047 86 53 1 32 117 113 196 1.77e-12 63.5
Msa0467380 MtrunA17_Chr3g0135111 35.652 115 60 4 27 136 1 106 5.12e-11 59.3
Msa0467380 MtrunA17_Chr8g0344491 44.737 76 41 1 38 113 149 223 1.78e-12 63.5
Msa0467380 MtrunA17_Chr1g0153181 35.644 101 59 2 19 114 10 109 2.34e-12 63.2
Msa0467380 MtrunA17_Chr7g0267571 39.326 89 51 2 34 121 210 296 3.70e-12 62.4
Msa0467380 MtrunA17_Chr4g0070231 43.421 76 42 1 38 113 133 207 4.19e-12 62.4
Msa0467380 MtrunA17_Chr8g0360551 42.857 70 40 0 33 102 70 139 6.76e-12 62.0
Msa0467380 MtrunA17_Chr1g0163591 40.000 100 56 3 35 133 161 257 9.67e-12 61.6
Msa0467380 MtrunA17_Chr5g0402191 38.824 85 49 2 33 117 1 82 2.24e-11 60.5
Msa0467380 MtrunA17_Chr8g0375101 36.471 85 51 1 33 117 1 82 2.25e-11 60.5
Msa0467380 MtrunA17_Chr4g0074711 34.906 106 65 3 31 133 160 264 2.42e-11 60.5
Msa0467380 MtrunA17_Chr3g0139111 40.909 88 48 3 38 124 147 231 5.56e-11 59.3
Msa0467380 MtrunA17_Chr2g0328791 41.176 85 46 3 38 120 110 192 6.31e-11 58.9
Msa0467380 MtrunA17_Chr8g0369281 37.662 77 47 1 38 113 469 545 7.07e-11 59.3
Msa0467380 MtrunA17_Chr4g0042151 33.333 117 66 5 3 116 16 123 7.35e-11 57.4
Msa0467380 MtrunA17_Chr4g0044541 37.500 80 49 1 35 114 33 111 7.87e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0467380 AT3G23830.2 61.290 124 43 4 1 123 1 120 5.62e-46 146
Msa0467380 AT3G23830.1 61.290 124 43 4 1 123 1 120 5.62e-46 146
Msa0467380 AT4G13850.3 61.290 124 43 4 1 123 1 120 2.32e-44 142
Msa0467380 AT4G13850.2 61.290 124 43 4 1 123 1 120 3.05e-44 142
Msa0467380 AT4G13850.1 61.290 124 43 4 1 123 1 120 3.54e-44 142
Msa0467380 AT4G13850.4 59.690 129 42 4 1 128 1 120 7.85e-41 133
Msa0467380 AT5G61030.2 57.025 121 45 3 1 116 1 119 8.60e-40 136
Msa0467380 AT5G61030.1 57.025 121 45 3 1 116 1 119 8.60e-40 136
Msa0467380 AT1G74230.1 54.783 115 48 2 1 115 1 111 1.50e-36 127
Msa0467380 AT5G47320.1 57.759 116 41 4 1 115 1 109 2.01e-33 117
Msa0467380 AT3G08000.1 47.107 121 54 3 9 121 7 125 8.11e-27 98.2
Msa0467380 AT5G06210.1 48.000 100 42 2 35 134 33 122 7.43e-22 85.5
Msa0467380 AT2G21660.1 48.101 79 38 2 37 114 9 85 1.49e-19 80.5
Msa0467380 AT5G59865.1 48.837 86 41 2 34 118 24 107 3.24e-19 77.8
Msa0467380 AT2G37220.1 48.889 90 40 3 36 124 204 288 5.16e-19 81.3
Msa0467380 AT2G37220.1 40.000 95 50 3 37 130 92 180 4.56e-11 59.7
Msa0467380 AT3G53460.1 50.588 85 39 2 37 120 258 340 7.91e-19 81.6
Msa0467380 AT3G53460.3 50.588 85 39 2 37 120 258 340 7.91e-19 81.6
Msa0467380 AT3G53460.2 50.588 85 39 2 37 120 258 340 7.91e-19 81.6
Msa0467380 AT3G53460.4 50.588 85 39 2 37 120 279 361 9.52e-19 81.3
Msa0467380 AT4G39260.3 41.176 85 47 2 37 120 7 89 8.28e-18 73.6
Msa0467380 AT3G26420.1 43.434 99 53 2 37 134 8 104 8.86e-18 77.4
Msa0467380 AT1G18630.1 40.870 115 62 3 1 114 4 113 8.91e-18 75.5
Msa0467380 AT3G46020.1 46.341 82 41 2 35 115 6 85 5.71e-17 72.0
Msa0467380 AT4G24770.1 46.914 81 40 2 37 116 245 323 1.06e-16 75.5
Msa0467380 AT4G24770.1 35.965 114 72 1 23 136 137 249 5.82e-12 62.4
Msa0467380 AT4G24770.2 46.914 81 40 2 37 116 245 323 1.06e-16 75.5
Msa0467380 AT4G24770.2 35.965 114 72 1 23 136 137 249 5.82e-12 62.4
Msa0467380 AT2G37510.1 46.479 71 37 1 33 102 31 101 1.63e-16 71.6
Msa0467380 AT2G21660.2 44.554 101 53 2 37 136 9 107 2.17e-16 72.0
Msa0467380 AT5G50250.1 44.444 81 42 2 37 116 208 286 3.19e-16 73.9
Msa0467380 AT5G50250.1 38.235 102 62 1 36 137 113 213 2.44e-12 63.2
Msa0467380 AT3G13224.3 39.560 91 54 1 28 118 92 181 1.07e-15 72.8
Msa0467380 AT3G13224.2 39.560 91 54 1 28 118 101 190 1.07e-15 72.8
Msa0467380 AT4G39260.2 41.026 78 43 2 37 113 7 82 1.28e-15 68.9
Msa0467380 AT4G39260.1 41.026 78 43 2 37 113 7 82 3.26e-15 68.9
Msa0467380 AT5G55550.5 43.210 81 43 2 37 117 7 84 5.06e-15 71.2
Msa0467380 AT5G55550.4 43.210 81 43 2 37 117 7 84 5.06e-15 71.2
Msa0467380 AT5G55550.9 43.210 81 43 2 37 117 7 84 5.06e-15 71.2
Msa0467380 AT5G55550.7 43.210 81 43 2 37 117 7 84 5.06e-15 71.2
Msa0467380 AT5G55550.1 43.210 81 43 2 37 117 7 84 5.06e-15 71.2
Msa0467380 AT5G55550.2 43.210 81 43 2 37 117 7 84 5.59e-15 71.2
Msa0467380 AT5G55550.3 43.210 81 43 2 37 117 7 84 5.59e-15 71.2
Msa0467380 AT5G55550.6 43.210 81 43 2 37 117 7 84 5.59e-15 71.2
Msa0467380 AT5G55550.11 43.210 81 43 2 37 117 7 84 5.59e-15 71.2
Msa0467380 AT4G13860.1 46.753 77 38 2 35 110 2 76 8.02e-15 65.9
Msa0467380 AT1G60000.1 50.617 81 37 2 34 113 175 253 1.25e-14 68.9
Msa0467380 AT1G76460.1 37.634 93 53 2 36 128 24 111 1.58e-14 68.9
Msa0467380 AT5G55550.8 46.970 66 34 1 37 102 7 71 1.61e-14 69.7
Msa0467380 AT5G55550.10 46.970 66 34 1 37 102 7 71 1.65e-14 69.7
Msa0467380 AT3G20930.1 36.458 96 58 2 33 127 279 372 1.88e-14 69.3
Msa0467380 AT3G07810.2 40.000 80 45 1 37 116 7 83 2.56e-14 69.3
Msa0467380 AT3G07810.1 40.000 80 45 1 37 116 7 83 2.79e-14 69.3
Msa0467380 AT3G13224.1 46.053 76 40 1 28 103 101 175 3.13e-14 67.8
Msa0467380 AT1G17640.2 35.294 102 64 2 34 135 155 254 3.48e-14 68.6
Msa0467380 AT1G17640.1 35.294 102 64 2 34 135 155 254 3.48e-14 68.6
Msa0467380 AT1G17640.3 35.294 102 64 2 34 135 155 254 3.48e-14 68.6
Msa0467380 AT1G22910.1 40.506 79 45 2 36 114 13 89 3.98e-14 67.8
Msa0467380 AT1G22910.2 40.506 79 45 2 36 114 13 89 4.20e-14 67.4
Msa0467380 AT3G52380.1 44.706 85 44 2 34 117 217 299 5.12e-14 68.2
Msa0467380 AT1G33470.2 41.791 67 39 0 36 102 7 73 5.67e-14 67.0
Msa0467380 AT1G33470.1 41.791 67 39 0 36 102 7 73 5.67e-14 67.0
Msa0467380 AT5G47620.1 44.000 75 38 1 32 102 2 76 5.77e-14 68.2
Msa0467380 AT5G47620.2 44.000 75 38 1 32 102 2 76 5.77e-14 68.2
Msa0467380 AT5G47620.5 44.000 75 38 1 32 102 2 76 5.77e-14 68.2
Msa0467380 AT1G33470.3 41.791 67 39 0 36 102 7 73 6.06e-14 67.0
Msa0467380 AT1G22910.3 38.776 98 55 3 36 133 13 105 6.81e-14 67.8
Msa0467380 AT1G20880.2 40.541 74 39 1 36 104 24 97 8.17e-14 67.0
Msa0467380 AT1G20880.3 40.541 74 39 1 36 104 24 97 8.17e-14 67.0
Msa0467380 AT1G20880.1 40.541 74 39 1 36 104 24 97 8.17e-14 67.0
Msa0467380 AT4G26650.1 42.500 80 43 2 37 116 16 92 8.20e-14 67.8
Msa0467380 AT4G26650.2 42.500 80 43 2 37 116 13 89 8.51e-14 67.8
Msa0467380 AT2G46780.1 36.449 107 65 2 36 142 22 125 9.11e-14 67.4
Msa0467380 AT2G46780.2 36.449 107 65 2 36 142 22 125 9.11e-14 67.4
Msa0467380 AT5G54580.1 36.134 119 62 4 3 117 28 136 9.36e-14 65.1
Msa0467380 AT4G39260.4 40.678 59 35 0 37 95 7 65 9.52e-14 63.5
Msa0467380 AT2G27330.1 34.021 97 61 2 35 130 20 114 1.17e-13 63.5
Msa0467380 AT5G47620.4 46.154 65 35 0 32 96 2 66 1.18e-13 67.4
Msa0467380 AT4G14300.1 39.506 81 48 1 37 117 7 86 1.49e-13 67.0
Msa0467380 AT4G14300.1 35.714 84 52 1 35 118 109 190 1.12e-11 61.6
Msa0467380 AT4G14300.2 39.506 81 48 1 37 117 7 86 1.49e-13 67.0
Msa0467380 AT4G14300.2 35.714 84 52 1 35 118 109 190 1.12e-11 61.6
Msa0467380 AT1G22330.1 33.981 103 67 1 36 138 17 118 1.64e-13 66.2
Msa0467380 AT1G71800.1 37.113 97 56 3 38 133 11 103 2.04e-13 66.6
Msa0467380 AT5G40490.1 34.884 86 55 1 33 118 127 211 2.33e-13 66.6
Msa0467380 AT5G40490.2 35.294 85 54 1 35 119 112 195 3.14e-13 66.2
Msa0467380 AT1G78260.1 35.526 76 49 0 36 111 17 92 3.67e-13 65.5
Msa0467380 AT2G21690.1 44.828 58 32 0 39 96 10 67 3.80e-13 62.4
Msa0467380 AT1G78260.2 38.806 67 41 0 36 102 17 83 4.71e-13 65.1
Msa0467380 AT1G60650.2 37.383 107 61 3 35 140 11 112 5.51e-13 65.1
Msa0467380 AT1G60650.1 37.383 107 61 3 35 140 11 112 5.51e-13 65.1
Msa0467380 AT1G54080.1 42.857 77 41 2 38 113 150 224 6.71e-13 65.1
Msa0467380 AT5G04280.1 38.356 73 39 1 36 108 7 73 9.76e-13 64.3
Msa0467380 AT2G18510.1 41.573 89 48 3 36 122 112 198 1.37e-12 64.3
Msa0467380 AT2G22100.1 35.106 94 59 1 31 124 158 249 1.71e-12 63.9
Msa0467380 AT2G33410.1 37.647 85 50 2 37 121 7 88 1.79e-12 63.9
Msa0467380 AT2G33410.1 32.759 116 68 3 11 118 77 190 6.88e-12 62.4
Msa0467380 AT1G48920.1 37.736 106 62 2 35 140 296 397 2.31e-12 63.5
Msa0467380 AT3G54770.3 38.028 71 42 1 36 106 17 85 2.64e-12 62.8
Msa0467380 AT3G14100.1 41.558 77 42 2 38 113 146 220 2.68e-12 63.5
Msa0467380 AT3G54770.1 38.028 71 42 1 36 106 17 85 3.03e-12 62.8
Msa0467380 AT3G20930.2 34.653 101 58 3 33 127 279 377 3.07e-12 63.2
Msa0467380 AT5G53720.1 33.333 93 62 0 35 127 8 100 3.75e-12 59.3
Msa0467380 AT1G47490.2 38.542 96 55 3 38 131 199 292 8.34e-12 61.6
Msa0467380 AT1G47490.1 38.542 96 55 3 38 131 199 292 8.47e-12 62.0
Msa0467380 AT4G13860.2 52.459 61 26 2 52 111 2 60 1.15e-11 57.4
Msa0467380 AT1G49760.1 41.772 79 45 1 35 113 44 121 1.73e-11 61.2
Msa0467380 AT1G49760.2 41.772 79 45 1 35 113 44 121 1.73e-11 61.2
Msa0467380 AT3G18610.1 37.624 101 58 3 35 135 383 478 1.75e-11 61.2
Msa0467380 AT4G00830.3 36.364 88 53 2 24 111 106 190 2.45e-11 60.8
Msa0467380 AT4G00830.5 36.364 88 53 2 24 111 106 190 2.45e-11 60.8
Msa0467380 AT3G15010.1 32.323 99 67 0 4 102 43 141 2.61e-11 60.5
Msa0467380 AT3G15010.2 32.323 99 67 0 4 102 43 141 2.61e-11 60.5
Msa0467380 AT5G53680.1 32.051 78 53 0 36 113 13 90 2.65e-11 58.9
Msa0467380 AT4G00830.2 36.364 88 53 2 24 111 106 190 2.68e-11 60.5
Msa0467380 AT4G00830.1 36.364 88 53 2 24 111 106 190 2.68e-11 60.5
Msa0467380 AT4G00830.6 36.364 88 53 2 24 111 106 190 2.68e-11 60.5
Msa0467380 AT1G47500.1 37.113 97 57 3 38 132 201 295 2.92e-11 60.5
Msa0467380 AT3G18610.3 37.624 101 58 3 35 135 353 448 3.19e-11 60.5
Msa0467380 AT2G19380.1 32.558 86 58 0 9 94 381 466 3.26e-11 60.5
Msa0467380 AT5G11412.1 34.615 78 49 1 36 113 52 127 3.59e-11 58.5
Msa0467380 AT1G47500.2 37.113 97 57 3 38 132 201 295 4.12e-11 59.7
Msa0467380 AT2G37510.2 34.375 96 37 2 33 102 31 126 4.83e-11 58.2
Msa0467380 AT3G18610.2 37.624 101 58 3 35 135 323 418 5.69e-11 59.7
Msa0467380 AT1G17370.2 35.922 103 56 3 38 132 141 241 5.82e-11 59.7
Msa0467380 AT4G34110.1 40.000 80 47 1 35 114 35 113 6.00e-11 59.7
Msa0467380 AT1G01080.1 36.782 87 53 1 24 110 200 284 6.25e-11 59.3
Msa0467380 AT1G01080.2 38.272 81 48 1 30 110 207 285 6.40e-11 59.3
Msa0467380 AT1G17370.1 41.558 77 42 2 38 113 141 215 6.48e-11 59.3
Msa0467380 AT1G54080.2 40.741 81 41 3 38 113 150 228 7.55e-11 59.3

Find 73 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTTCATTAGGATTTAAA+TGG 0.219990 3_3:-68648877 Msa0467380:intron
TTGTTGTCGTTGACACATTT+TGG 0.256389 3_3:-68648606 Msa0467380:three_prime_UTR
TTGTGGTTATGTCAATAATC+TGG 0.263577 3_3:-68649797 Msa0467380:five_prime_UTR
AGCTAAGTCTTCAATCAAAA+TGG 0.296905 3_3:-68649738 Msa0467380:exon
ACTGGAAGATCAAGGGGATT+TGG 0.334462 3_3:-68649027 Msa0467380:CDS
TACTAATTAGTGAGATGTTA+TGG 0.340024 3_3:-68648633 Msa0467380:three_prime_UTR
GGCTACAGCGGCGGCGGCGG+TGG 0.351126 3_3:-68648793 Msa0467380:CDS
GGTGGATACGGCGGTGGTGG+CGG 0.366905 3_3:-68648754 Msa0467380:CDS
GGTGGCTACAGCGGCGGCGG+CGG 0.367068 3_3:-68648796 Msa0467380:CDS
GGATACAGCGGCGGCGGCGG+TGG 0.370470 3_3:-68648772 Msa0467380:CDS
ATGGCTTTCTGTAACAAGAT+TGG 0.370753 3_3:-68649719 Msa0467380:CDS
AGCGGCGGCGGCGGTGGATA+CGG 0.384390 3_3:-68648766 Msa0467380:CDS
GGCGGTGGATACGGCGGTGG+TGG 0.403299 3_3:-68648757 Msa0467380:CDS
TGTTGATGACCAGTCTCTTA+AGG 0.428494 3_3:-68649489 Msa0467380:CDS
ACGGCGGTGGTGGCGGCGGT+TGG 0.433529 3_3:-68648747 Msa0467380:CDS
GGTGGATACAGCGGCGGCGG+CGG 0.434113 3_3:-68648775 Msa0467380:CDS
AAAATAAGATAATAGAATGT+TGG 0.440908 3_3:-68648544 Msa0467380:three_prime_UTR
GGCGGTGGATACAGCGGCGG+CGG 0.441179 3_3:-68648778 Msa0467380:CDS
ACCGGGACACAGTAAGATAA+TGG 0.443037 3_3:+68648421 None:intergenic
TGTGGTGTAATCATAATAAT+CGG 0.443694 3_3:+68648362 None:intergenic
GGCGGCGGTGGATACGGCGG+TGG 0.444371 3_3:-68648760 Msa0467380:CDS
TCCATTATCTTACTGTGTCC+CGG 0.448342 3_3:-68648422 Msa0467380:three_prime_UTR
GCCGCCGCTGTAGCCACCTT+CGG 0.450300 3_3:+68648801 None:intergenic
GGATACGGCGGTGGTGGCGG+CGG 0.459808 3_3:-68648751 Msa0467380:CDS
TTGTTCATTAGGATTTAAAT+GGG 0.460304 3_3:-68648876 Msa0467380:intron
TAGTTAAGCATGGATGAAAC+AGG 0.461015 3_3:+68649656 None:intergenic
GTCCCGGTCACATCATTTGA+TGG 0.471438 3_3:-68648406 Msa0467380:three_prime_UTR
TGTGGTTATGTCAATAATCT+GGG 0.480742 3_3:-68649796 Msa0467380:five_prime_UTR
AGCACTCTCCATGGATGGAC+AGG 0.490812 3_3:-68648968 Msa0467380:intron
GAAGGTGGCTACAGCGGCGG+CGG 0.511885 3_3:-68648799 Msa0467380:CDS
ATAGAGACACTGGAAGATCA+AGG 0.517492 3_3:-68649035 Msa0467380:CDS
CCACCATCAAATGATGTGAC+CGG 0.522354 3_3:+68648403 None:intergenic
GTCATATGCAAATGATAGAC+AGG 0.524217 3_3:-68648839 Msa0467380:CDS
TAAATGCATCCTTAAGAGAC+TGG 0.532048 3_3:+68649480 None:intergenic
AAGGATGCATTTACAACCTA+CGG 0.532917 3_3:-68649470 Msa0467380:CDS
TTATGTTATTGTTATAATTG+TGG 0.538133 3_3:-68649814 Msa0467380:five_prime_UTR
GGTCATCAACATGGTAAGAA+AGG 0.538171 3_3:+68649501 None:intergenic
TATGAACTTGTTATGGCGAG+TGG 0.538491 3_3:-68648661 Msa0467380:three_prime_UTR
ATCATTTGATGGTGGTGCAT+TGG 0.538715 3_3:-68648395 Msa0467380:three_prime_UTR
TACACCACATCATTTGATGA+TGG 0.538977 3_3:-68648348 Msa0467380:three_prime_UTR
GGTGGACCTCGTTCCGAAGG+TGG 0.542049 3_3:-68648814 Msa0467380:CDS
CACCATCAAATGATGTGACC+GGG 0.548514 3_3:+68648404 None:intergenic
TATTACAACCTGTCCATCCA+TGG 0.550532 3_3:+68648960 None:intergenic
TGAAGTTGCAGACTCTTCAC+TGG 0.556568 3_3:+68648990 None:intergenic
TGCAACTTCAGCACTCTCCA+TGG 0.559718 3_3:-68648977 Msa0467380:CDS
AACCTACGGAGAAGTGGTTG+AGG 0.563954 3_3:-68649456 Msa0467380:CDS
ACTTCAGCACTCTCCATGGA+TGG 0.565520 3_3:-68648973 Msa0467380:CDS
GCTGGTGGACCTCGTTCCGA+AGG 0.574021 3_3:-68648817 Msa0467380:CDS
TCCGAAGGTGGCTACAGCGG+CGG 0.577472 3_3:-68648802 Msa0467380:CDS
GAGTAGCACCTTGCCTCAAG+AGG 0.579009 3_3:+68649693 None:intergenic
GTTATCACTGATAGAGACAC+TGG 0.581409 3_3:-68649045 Msa0467380:CDS
GGCGGCGGTGGATACAGCGG+CGG 0.582739 3_3:-68648781 Msa0467380:CDS
TATGCAAATGATAGACAGGC+TGG 0.584408 3_3:-68648835 Msa0467380:CDS
TCTGTTTATGAGAATAGTCG+TGG 0.591571 3_3:-68648498 Msa0467380:three_prime_UTR
TAGAGACACTGGAAGATCAA+GGG 0.594765 3_3:-68649034 Msa0467380:CDS
GGCGGCGGCGGTGGATACAG+CGG 0.598077 3_3:-68648784 Msa0467380:CDS
CTGTAGCCACCTTCGGAACG+AGG 0.599008 3_3:+68648808 None:intergenic
CAGTAAGATAATGGAAACAT+TGG 0.602106 3_3:+68648430 None:intergenic
ACCTACGGAGAAGTGGTTGA+GGG 0.603405 3_3:-68649455 Msa0467380:intron
AGAGACACTGGAAGATCAAG+GGG 0.604078 3_3:-68649033 Msa0467380:CDS
ATTTACAACCTACGGAGAAG+TGG 0.607456 3_3:-68649462 Msa0467380:CDS
CGTTCCGAAGGTGGCTACAG+CGG 0.613095 3_3:-68648805 Msa0467380:CDS
GGCGGCGGCGGTGGATACGG+CGG 0.621813 3_3:-68648763 Msa0467380:CDS
GTGGCGCAAGTAGTTAAGCA+TGG 0.622132 3_3:+68649646 None:intergenic
ATTGGAAACCTCTTGAGGCA+AGG 0.631105 3_3:-68649701 Msa0467380:CDS
TAAGAGACTGGTCATCAACA+TGG 0.631495 3_3:+68649492 None:intergenic
TAGACACAAGAACTCAACAC+AGG 0.651558 3_3:+68648691 None:intergenic
ACAAGATTGGAAACCTCTTG+AGG 0.658250 3_3:-68649706 Msa0467380:CDS
ACCCTCAACCACTTCTCCGT+AGG 0.658961 3_3:+68649454 None:intergenic
CCGGTCACATCATTTGATGG+TGG 0.675424 3_3:-68648403 Msa0467380:three_prime_UTR
GCAAATGATAGACAGGCTGG+TGG 0.676461 3_3:-68648832 Msa0467380:CDS
TGCACCATCATCAAATGATG+TGG 0.684822 3_3:+68648344 None:intergenic
AAAGCTTGCTTGAAGACATG+TGG 0.718559 3_3:+68649627 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTATAATAAATAACTT+TGG - chr3_3:68649065-68649084 Msa0467380:intron 10.0%
!!! GTTTTTATAATAAATAACTT+TGG - chr3_3:68649065-68649084 Msa0467380:intron 10.0%
!! TTATGTTATTGTTATAATTG+TGG - chr3_3:68648338-68648357 Msa0467380:three_prime_UTR 15.0%
!!! ATTTTAATTGTCATTTTAGT+CGG - chr3_3:68649238-68649257 Msa0467380:intron 15.0%
!!! AAAATTTTATGAACTTGTTA+TGG - chr3_3:68649484-68649503 Msa0467380:CDS 15.0%
!! AAAATAAGATAATAGAATGT+TGG - chr3_3:68649608-68649627 Msa0467380:intron 15.0%
!! TTATGTTATTGTTATAATTG+TGG - chr3_3:68648338-68648357 Msa0467380:three_prime_UTR 15.0%
!!! ATTTTAATTGTCATTTTAGT+CGG - chr3_3:68649238-68649257 Msa0467380:intron 15.0%
!!! AAAATTTTATGAACTTGTTA+TGG - chr3_3:68649484-68649503 Msa0467380:CDS 15.0%
!! AAAATAAGATAATAGAATGT+TGG - chr3_3:68649608-68649627 Msa0467380:intron 15.0%
!!! TTACTTTTATATTAGGAACT+TGG - chr3_3:68648383-68648402 Msa0467380:three_prime_UTR 20.0%
!!! TTTATCTAGTTTTTTTATGC+TGG - chr3_3:68648570-68648589 Msa0467380:three_prime_UTR 20.0%
!!! TCTAATAAAATGTATGGTTT+TGG - chr3_3:68648612-68648631 Msa0467380:three_prime_UTR 20.0%
!! GAATGTTAGAAGTTAAATTT+GGG - chr3_3:68648817-68648836 Msa0467380:CDS 20.0%
!! TTGTTCATTAGGATTTAAAT+GGG - chr3_3:68649276-68649295 Msa0467380:intron 20.0%
!!! TGGTTTTTTAGTGTTTTTAT+AGG - chr3_3:68649566-68649585 Msa0467380:intron 20.0%
!!! TTACTTTTATATTAGGAACT+TGG - chr3_3:68648383-68648402 Msa0467380:three_prime_UTR 20.0%
!!! TTTATCTAGTTTTTTTATGC+TGG - chr3_3:68648570-68648589 Msa0467380:three_prime_UTR 20.0%
!!! TCTAATAAAATGTATGGTTT+TGG - chr3_3:68648612-68648631 Msa0467380:three_prime_UTR 20.0%
!! GAATGTTAGAAGTTAAATTT+GGG - chr3_3:68648817-68648836 Msa0467380:CDS 20.0%
!! TTGTTCATTAGGATTTAAAT+GGG - chr3_3:68649276-68649295 Msa0467380:intron 20.0%
!!! TGGTTTTTTAGTGTTTTTAT+AGG - chr3_3:68649566-68649585 Msa0467380:intron 20.0%
!!! GGGTTTGTTACTTTTATATT+AGG - chr3_3:68648376-68648395 Msa0467380:three_prime_UTR 25.0%
!! TTTTATATTAGGAACTTGGA+AGG - chr3_3:68648387-68648406 Msa0467380:three_prime_UTR 25.0%
! GCTGGTTAGTATTATTATTA+TGG - chr3_3:68648588-68648607 Msa0467380:three_prime_UTR 25.0%
!!! TATGGCTCTAATAAAATGTA+TGG - chr3_3:68648606-68648625 Msa0467380:three_prime_UTR 25.0%
!!! AAATGTATGGTTTTGGATTT+TGG - chr3_3:68648619-68648638 Msa0467380:three_prime_UTR 25.0%
!! AGTCTTATGTTGATACAAAA+GGG + chr3_3:68648785-68648804 None:intergenic 25.0%
! GGAATGTTAGAAGTTAAATT+TGG - chr3_3:68648816-68648835 Msa0467380:CDS 25.0%
!! TTGATACATTAAAGTGTCTT+TGG - chr3_3:68648991-68649010 Msa0467380:CDS 25.0%
! CTTGTTCATTAGGATTTAAA+TGG - chr3_3:68649275-68649294 Msa0467380:intron 25.0%
! TACTAATTAGTGAGATGTTA+TGG - chr3_3:68649519-68649538 Msa0467380:intron 25.0%
!! GCAGTATTTAGGTATATTTT+TGG - chr3_3:68649687-68649706 Msa0467380:CDS 25.0%
!!! GTATTTAGGTATATTTTTGG+TGG - chr3_3:68649690-68649709 Msa0467380:CDS 25.0%
! TGTGGTGTAATCATAATAAT+CGG + chr3_3:68649793-68649812 None:intergenic 25.0%
!!! GGGTTTGTTACTTTTATATT+AGG - chr3_3:68648376-68648395 Msa0467380:three_prime_UTR 25.0%
!! TTTTATATTAGGAACTTGGA+AGG - chr3_3:68648387-68648406 Msa0467380:three_prime_UTR 25.0%
! GCTGGTTAGTATTATTATTA+TGG - chr3_3:68648588-68648607 Msa0467380:three_prime_UTR 25.0%
!!! TATGGCTCTAATAAAATGTA+TGG - chr3_3:68648606-68648625 Msa0467380:three_prime_UTR 25.0%
!!! AAATGTATGGTTTTGGATTT+TGG - chr3_3:68648619-68648638 Msa0467380:three_prime_UTR 25.0%
!! AGTCTTATGTTGATACAAAA+GGG + chr3_3:68648785-68648804 None:intergenic 25.0%
! GGAATGTTAGAAGTTAAATT+TGG - chr3_3:68648816-68648835 Msa0467380:CDS 25.0%
!! TTGATACATTAAAGTGTCTT+TGG - chr3_3:68648991-68649010 Msa0467380:CDS 25.0%
! CTTGTTCATTAGGATTTAAA+TGG - chr3_3:68649275-68649294 Msa0467380:intron 25.0%
! TACTAATTAGTGAGATGTTA+TGG - chr3_3:68649519-68649538 Msa0467380:intron 25.0%
!! GCAGTATTTAGGTATATTTT+TGG - chr3_3:68649687-68649706 Msa0467380:CDS 25.0%
!!! GTATTTAGGTATATTTTTGG+TGG - chr3_3:68649690-68649709 Msa0467380:CDS 25.0%
! TGTGGTGTAATCATAATAAT+CGG + chr3_3:68649793-68649812 None:intergenic 25.0%
TTGTGGTTATGTCAATAATC+TGG - chr3_3:68648355-68648374 Msa0467380:three_prime_UTR 30.0%
TGTGGTTATGTCAATAATCT+GGG - chr3_3:68648356-68648375 Msa0467380:three_prime_UTR 30.0%
AGCTAAGTCTTCAATCAAAA+TGG - chr3_3:68648414-68648433 Msa0467380:three_prime_UTR 30.0%
!! TTTCATTGGAGGTATTGATT+TGG - chr3_3:68648546-68648565 Msa0467380:three_prime_UTR 30.0%
GTCTTATGTTGATACAAAAG+GGG + chr3_3:68648784-68648803 None:intergenic 30.0%
! CAGTCTTATGTTGATACAAA+AGG + chr3_3:68648786-68648805 None:intergenic 30.0%
TGAAGAGTAAGTCAAATTTC+CGG - chr3_3:68648965-68648984 Msa0467380:intron 30.0%
CACTTTAATGTATCAACTAC+CGG + chr3_3:68648987-68649006 None:intergenic 30.0%
!! CAGTAAGATAATGGAAACAT+TGG + chr3_3:68649725-68649744 None:intergenic 30.0%
TTGTGGTTATGTCAATAATC+TGG - chr3_3:68648355-68648374 Msa0467380:three_prime_UTR 30.0%
TGTGGTTATGTCAATAATCT+GGG - chr3_3:68648356-68648375 Msa0467380:three_prime_UTR 30.0%
AGCTAAGTCTTCAATCAAAA+TGG - chr3_3:68648414-68648433 Msa0467380:three_prime_UTR 30.0%
!! TTTCATTGGAGGTATTGATT+TGG - chr3_3:68648546-68648565 Msa0467380:three_prime_UTR 30.0%
GTCTTATGTTGATACAAAAG+GGG + chr3_3:68648784-68648803 None:intergenic 30.0%
! CAGTCTTATGTTGATACAAA+AGG + chr3_3:68648786-68648805 None:intergenic 30.0%
TGAAGAGTAAGTCAAATTTC+CGG - chr3_3:68648965-68648984 Msa0467380:intron 30.0%
CACTTTAATGTATCAACTAC+CGG + chr3_3:68648987-68649006 None:intergenic 30.0%
!! CAGTAAGATAATGGAAACAT+TGG + chr3_3:68649725-68649744 None:intergenic 30.0%
ATGGCTTTCTGTAACAAGAT+TGG - chr3_3:68648433-68648452 Msa0467380:three_prime_UTR 35.0%
TAGTTAAGCATGGATGAAAC+AGG + chr3_3:68648499-68648518 None:intergenic 35.0%
! TCTTCAAGCAAGCTTTTCAT+TGG - chr3_3:68648532-68648551 Msa0467380:three_prime_UTR 35.0%
!! GTTTTGGATTTTGGTATCAC+AGG - chr3_3:68648628-68648647 Msa0467380:three_prime_UTR 35.0%
TAAATGCATCCTTAAGAGAC+TGG + chr3_3:68648675-68648694 None:intergenic 35.0%
AAGGATGCATTTACAACCTA+CGG - chr3_3:68648682-68648701 Msa0467380:three_prime_UTR 35.0%
! ACATAAGACTGCATTGACAT+TGG - chr3_3:68648795-68648814 Msa0467380:CDS 35.0%
!! GCATCTGCTTTGTTTGATTA+TGG - chr3_3:68649041-68649060 Msa0467380:CDS 35.0%
CTGACATACTCTTGTTCATT+AGG - chr3_3:68649265-68649284 Msa0467380:intron 35.0%
GTCATATGCAAATGATAGAC+AGG - chr3_3:68649313-68649332 Msa0467380:intron 35.0%
! TTGTTGTCGTTGACACATTT+TGG - chr3_3:68649546-68649565 Msa0467380:intron 35.0%
TCTGTTTATGAGAATAGTCG+TGG - chr3_3:68649654-68649673 Msa0467380:CDS 35.0%
GCACAAAAACTGCAGTATTT+AGG - chr3_3:68649676-68649695 Msa0467380:CDS 35.0%
TACACCACATCATTTGATGA+TGG - chr3_3:68649804-68649823 Msa0467380:five_prime_UTR 35.0%
ATGGCTTTCTGTAACAAGAT+TGG - chr3_3:68648433-68648452 Msa0467380:three_prime_UTR 35.0%
TAGTTAAGCATGGATGAAAC+AGG + chr3_3:68648499-68648518 None:intergenic 35.0%
! TCTTCAAGCAAGCTTTTCAT+TGG - chr3_3:68648532-68648551 Msa0467380:three_prime_UTR 35.0%
!! GTTTTGGATTTTGGTATCAC+AGG - chr3_3:68648628-68648647 Msa0467380:three_prime_UTR 35.0%
TAAATGCATCCTTAAGAGAC+TGG + chr3_3:68648675-68648694 None:intergenic 35.0%
AAGGATGCATTTACAACCTA+CGG - chr3_3:68648682-68648701 Msa0467380:three_prime_UTR 35.0%
! ACATAAGACTGCATTGACAT+TGG - chr3_3:68648795-68648814 Msa0467380:CDS 35.0%
!! GCATCTGCTTTGTTTGATTA+TGG - chr3_3:68649041-68649060 Msa0467380:CDS 35.0%
CTGACATACTCTTGTTCATT+AGG - chr3_3:68649265-68649284 Msa0467380:intron 35.0%
GTCATATGCAAATGATAGAC+AGG - chr3_3:68649313-68649332 Msa0467380:intron 35.0%
! TTGTTGTCGTTGACACATTT+TGG - chr3_3:68649546-68649565 Msa0467380:intron 35.0%
TCTGTTTATGAGAATAGTCG+TGG - chr3_3:68649654-68649673 Msa0467380:CDS 35.0%
GCACAAAAACTGCAGTATTT+AGG - chr3_3:68649676-68649695 Msa0467380:CDS 35.0%
TACACCACATCATTTGATGA+TGG - chr3_3:68649804-68649823 Msa0467380:five_prime_UTR 35.0%
ACAAGATTGGAAACCTCTTG+AGG - chr3_3:68648446-68648465 Msa0467380:three_prime_UTR 40.0%
! AAAGCTTGCTTGAAGACATG+TGG + chr3_3:68648528-68648547 None:intergenic 40.0%
! TCAAGCAAGCTTTTCATTGG+AGG - chr3_3:68648535-68648554 Msa0467380:three_prime_UTR 40.0%
GGTCATCAACATGGTAAGAA+AGG + chr3_3:68648654-68648673 None:intergenic 40.0%
! TAAGAGACTGGTCATCAACA+TGG + chr3_3:68648663-68648682 None:intergenic 40.0%
!! TGTTGATGACCAGTCTCTTA+AGG - chr3_3:68648663-68648682 Msa0467380:three_prime_UTR 40.0%
ATTTACAACCTACGGAGAAG+TGG - chr3_3:68648690-68648709 Msa0467380:three_prime_UTR 40.0%
TGCTGAAGAACACATTTCAG+TGG - chr3_3:68648854-68648873 Msa0467380:CDS 40.0%
GCTGAAGAACACATTTCAGT+GGG - chr3_3:68648855-68648874 Msa0467380:CDS 40.0%
CTGAAGAACACATTTCAGTG+GGG - chr3_3:68648856-68648875 Msa0467380:CDS 40.0%
GTTATCACTGATAGAGACAC+TGG - chr3_3:68649107-68649126 Msa0467380:intron 40.0%
ATAGAGACACTGGAAGATCA+AGG - chr3_3:68649117-68649136 Msa0467380:intron 40.0%
TAGAGACACTGGAAGATCAA+GGG - chr3_3:68649118-68649137 Msa0467380:intron 40.0%
TATTACAACCTGTCCATCCA+TGG + chr3_3:68649195-68649214 None:intergenic 40.0%
TATGCAAATGATAGACAGGC+TGG - chr3_3:68649317-68649336 Msa0467380:intron 40.0%
TAGACACAAGAACTCAACAC+AGG + chr3_3:68649464-68649483 None:intergenic 40.0%
!! TATGAACTTGTTATGGCGAG+TGG - chr3_3:68649491-68649510 Msa0467380:CDS 40.0%
TCCATTATCTTACTGTGTCC+CGG - chr3_3:68649730-68649749 Msa0467380:exon 40.0%
!! ATCATTTGATGGTGGTGCAT+TGG - chr3_3:68649757-68649776 Msa0467380:five_prime_UTR 40.0%
TGCACCATCATCAAATGATG+TGG + chr3_3:68649811-68649830 None:intergenic 40.0%
ACAAGATTGGAAACCTCTTG+AGG - chr3_3:68648446-68648465 Msa0467380:three_prime_UTR 40.0%
! AAAGCTTGCTTGAAGACATG+TGG + chr3_3:68648528-68648547 None:intergenic 40.0%
! TCAAGCAAGCTTTTCATTGG+AGG - chr3_3:68648535-68648554 Msa0467380:three_prime_UTR 40.0%
GGTCATCAACATGGTAAGAA+AGG + chr3_3:68648654-68648673 None:intergenic 40.0%
! TAAGAGACTGGTCATCAACA+TGG + chr3_3:68648663-68648682 None:intergenic 40.0%
!! TGTTGATGACCAGTCTCTTA+AGG - chr3_3:68648663-68648682 Msa0467380:three_prime_UTR 40.0%
ATTTACAACCTACGGAGAAG+TGG - chr3_3:68648690-68648709 Msa0467380:three_prime_UTR 40.0%
TGCTGAAGAACACATTTCAG+TGG - chr3_3:68648854-68648873 Msa0467380:CDS 40.0%
GCTGAAGAACACATTTCAGT+GGG - chr3_3:68648855-68648874 Msa0467380:CDS 40.0%
CTGAAGAACACATTTCAGTG+GGG - chr3_3:68648856-68648875 Msa0467380:CDS 40.0%
GTTATCACTGATAGAGACAC+TGG - chr3_3:68649107-68649126 Msa0467380:intron 40.0%
ATAGAGACACTGGAAGATCA+AGG - chr3_3:68649117-68649136 Msa0467380:intron 40.0%
TAGAGACACTGGAAGATCAA+GGG - chr3_3:68649118-68649137 Msa0467380:intron 40.0%
TATTACAACCTGTCCATCCA+TGG + chr3_3:68649195-68649214 None:intergenic 40.0%
TATGCAAATGATAGACAGGC+TGG - chr3_3:68649317-68649336 Msa0467380:intron 40.0%
TAGACACAAGAACTCAACAC+AGG + chr3_3:68649464-68649483 None:intergenic 40.0%
!! TATGAACTTGTTATGGCGAG+TGG - chr3_3:68649491-68649510 Msa0467380:CDS 40.0%
TCCATTATCTTACTGTGTCC+CGG - chr3_3:68649730-68649749 Msa0467380:exon 40.0%
!! ATCATTTGATGGTGGTGCAT+TGG - chr3_3:68649757-68649776 Msa0467380:five_prime_UTR 40.0%
TGCACCATCATCAAATGATG+TGG + chr3_3:68649811-68649830 None:intergenic 40.0%
ATTGGAAACCTCTTGAGGCA+AGG - chr3_3:68648451-68648470 Msa0467380:three_prime_UTR 45.0%
AGAGACACTGGAAGATCAAG+GGG - chr3_3:68649119-68649138 Msa0467380:intron 45.0%
ACTGGAAGATCAAGGGGATT+TGG - chr3_3:68649125-68649144 Msa0467380:intron 45.0%
TGAAGTTGCAGACTCTTCAC+TGG + chr3_3:68649165-68649184 None:intergenic 45.0%
ACCGGGACACAGTAAGATAA+TGG + chr3_3:68649734-68649753 None:intergenic 45.0%
CACCATCAAATGATGTGACC+GGG + chr3_3:68649751-68649770 None:intergenic 45.0%
CCACCATCAAATGATGTGAC+CGG + chr3_3:68649752-68649771 None:intergenic 45.0%
ATTGGAAACCTCTTGAGGCA+AGG - chr3_3:68648451-68648470 Msa0467380:three_prime_UTR 45.0%
AGAGACACTGGAAGATCAAG+GGG - chr3_3:68649119-68649138 Msa0467380:intron 45.0%
ACTGGAAGATCAAGGGGATT+TGG - chr3_3:68649125-68649144 Msa0467380:intron 45.0%
TGAAGTTGCAGACTCTTCAC+TGG + chr3_3:68649165-68649184 None:intergenic 45.0%
ACCGGGACACAGTAAGATAA+TGG + chr3_3:68649734-68649753 None:intergenic 45.0%
CACCATCAAATGATGTGACC+GGG + chr3_3:68649751-68649770 None:intergenic 45.0%
CCACCATCAAATGATGTGAC+CGG + chr3_3:68649752-68649771 None:intergenic 45.0%
GTGGCGCAAGTAGTTAAGCA+TGG + chr3_3:68648509-68648528 None:intergenic 50.0%
AACCTACGGAGAAGTGGTTG+AGG - chr3_3:68648696-68648715 Msa0467380:three_prime_UTR 50.0%
ACCTACGGAGAAGTGGTTGA+GGG - chr3_3:68648697-68648716 Msa0467380:three_prime_UTR 50.0%
TGCAACTTCAGCACTCTCCA+TGG - chr3_3:68649175-68649194 Msa0467380:intron 50.0%
ACTTCAGCACTCTCCATGGA+TGG - chr3_3:68649179-68649198 Msa0467380:intron 50.0%
GCAAATGATAGACAGGCTGG+TGG - chr3_3:68649320-68649339 Msa0467380:intron 50.0%
GTCCCGGTCACATCATTTGA+TGG - chr3_3:68649746-68649765 Msa0467380:five_prime_UTR 50.0%
CCGGTCACATCATTTGATGG+TGG - chr3_3:68649749-68649768 Msa0467380:five_prime_UTR 50.0%
GTGGCGCAAGTAGTTAAGCA+TGG + chr3_3:68648509-68648528 None:intergenic 50.0%
AACCTACGGAGAAGTGGTTG+AGG - chr3_3:68648696-68648715 Msa0467380:three_prime_UTR 50.0%
ACCTACGGAGAAGTGGTTGA+GGG - chr3_3:68648697-68648716 Msa0467380:three_prime_UTR 50.0%
TGCAACTTCAGCACTCTCCA+TGG - chr3_3:68649175-68649194 Msa0467380:intron 50.0%
ACTTCAGCACTCTCCATGGA+TGG - chr3_3:68649179-68649198 Msa0467380:intron 50.0%
GCAAATGATAGACAGGCTGG+TGG - chr3_3:68649320-68649339 Msa0467380:intron 50.0%
GTCCCGGTCACATCATTTGA+TGG - chr3_3:68649746-68649765 Msa0467380:five_prime_UTR 50.0%
CCGGTCACATCATTTGATGG+TGG - chr3_3:68649749-68649768 Msa0467380:five_prime_UTR 50.0%
GAGTAGCACCTTGCCTCAAG+AGG + chr3_3:68648462-68648481 None:intergenic 55.0%
ACCCTCAACCACTTCTCCGT+AGG + chr3_3:68648701-68648720 None:intergenic 55.0%
AGCACTCTCCATGGATGGAC+AGG - chr3_3:68649184-68649203 Msa0467380:intron 55.0%
GAGTAGCACCTTGCCTCAAG+AGG + chr3_3:68648462-68648481 None:intergenic 55.0%
ACCCTCAACCACTTCTCCGT+AGG + chr3_3:68648701-68648720 None:intergenic 55.0%
AGCACTCTCCATGGATGGAC+AGG - chr3_3:68649184-68649203 Msa0467380:intron 55.0%
CTGTAGCCACCTTCGGAACG+AGG + chr3_3:68649347-68649366 None:intergenic 60.0%
CGTTCCGAAGGTGGCTACAG+CGG - chr3_3:68649347-68649366 Msa0467380:intron 60.0%
CTGTAGCCACCTTCGGAACG+AGG + chr3_3:68649347-68649366 None:intergenic 60.0%
CGTTCCGAAGGTGGCTACAG+CGG - chr3_3:68649347-68649366 Msa0467380:intron 60.0%
! GCTGGTGGACCTCGTTCCGA+AGG - chr3_3:68649335-68649354 Msa0467380:intron 65.0%
GGTGGACCTCGTTCCGAAGG+TGG - chr3_3:68649338-68649357 Msa0467380:intron 65.0%
TCCGAAGGTGGCTACAGCGG+CGG - chr3_3:68649350-68649369 Msa0467380:intron 65.0%
! GCTGGTGGACCTCGTTCCGA+AGG - chr3_3:68649335-68649354 Msa0467380:intron 65.0%
GGTGGACCTCGTTCCGAAGG+TGG - chr3_3:68649338-68649357 Msa0467380:intron 65.0%
TCCGAAGGTGGCTACAGCGG+CGG - chr3_3:68649350-68649369 Msa0467380:intron 65.0%
GCCGCCGCTGTAGCCACCTT+CGG + chr3_3:68649354-68649373 None:intergenic 70.0%
GAAGGTGGCTACAGCGGCGG+CGG - chr3_3:68649353-68649372 Msa0467380:intron 70.0%
! GGTGGATACGGCGGTGGTGG+CGG - chr3_3:68649398-68649417 Msa0467380:intron 70.0%
GCCGCCGCTGTAGCCACCTT+CGG + chr3_3:68649354-68649373 None:intergenic 70.0%
GAAGGTGGCTACAGCGGCGG+CGG - chr3_3:68649353-68649372 Msa0467380:intron 70.0%
! GGTGGATACGGCGGTGGTGG+CGG - chr3_3:68649398-68649417 Msa0467380:intron 70.0%
GGCGGCGGCGGTGGATACAG+CGG - chr3_3:68649368-68649387 Msa0467380:intron 75.0%
GGCGGCGGTGGATACAGCGG+CGG - chr3_3:68649371-68649390 Msa0467380:intron 75.0%
GGCGGTGGATACAGCGGCGG+CGG - chr3_3:68649374-68649393 Msa0467380:intron 75.0%
GGTGGATACAGCGGCGGCGG+CGG - chr3_3:68649377-68649396 Msa0467380:intron 75.0%
AGCGGCGGCGGCGGTGGATA+CGG - chr3_3:68649365-68649384 Msa0467380:intron 75.0%
GGCGGTGGATACGGCGGTGG+TGG - chr3_3:68649395-68649414 Msa0467380:intron 75.0%
!! GGATACGGCGGTGGTGGCGG+CGG - chr3_3:68649401-68649420 Msa0467380:intron 75.0%
GGCGGCGGCGGTGGATACAG+CGG - chr3_3:68649368-68649387 Msa0467380:intron 75.0%
GGCGGCGGTGGATACAGCGG+CGG - chr3_3:68649371-68649390 Msa0467380:intron 75.0%
GGCGGTGGATACAGCGGCGG+CGG - chr3_3:68649374-68649393 Msa0467380:intron 75.0%
GGTGGATACAGCGGCGGCGG+CGG - chr3_3:68649377-68649396 Msa0467380:intron 75.0%
AGCGGCGGCGGCGGTGGATA+CGG - chr3_3:68649365-68649384 Msa0467380:intron 75.0%
GGCGGTGGATACGGCGGTGG+TGG - chr3_3:68649395-68649414 Msa0467380:intron 75.0%
!! GGATACGGCGGTGGTGGCGG+CGG - chr3_3:68649401-68649420 Msa0467380:intron 75.0%
! GGTGGCTACAGCGGCGGCGG+CGG - chr3_3:68649356-68649375 Msa0467380:intron 80.0%
! GGATACAGCGGCGGCGGCGG+TGG - chr3_3:68649380-68649399 Msa0467380:intron 80.0%
! GGCGGCGGCGGTGGATACGG+CGG - chr3_3:68649389-68649408 Msa0467380:intron 80.0%
! GGCGGCGGTGGATACGGCGG+TGG - chr3_3:68649392-68649411 Msa0467380:intron 80.0%
!!! ACGGCGGTGGTGGCGGCGGT+TGG - chr3_3:68649405-68649424 Msa0467380:intron 80.0%
! GGTGGCTACAGCGGCGGCGG+CGG - chr3_3:68649356-68649375 Msa0467380:intron 80.0%
! GGATACAGCGGCGGCGGCGG+TGG - chr3_3:68649380-68649399 Msa0467380:intron 80.0%
! GGCGGCGGCGGTGGATACGG+CGG - chr3_3:68649389-68649408 Msa0467380:intron 80.0%
! GGCGGCGGTGGATACGGCGG+TGG - chr3_3:68649392-68649411 Msa0467380:intron 80.0%
!!! ACGGCGGTGGTGGCGGCGGT+TGG - chr3_3:68649405-68649424 Msa0467380:intron 80.0%
! GGCTACAGCGGCGGCGGCGG+TGG - chr3_3:68649359-68649378 Msa0467380:intron 85.0%
! GGCTACAGCGGCGGCGGCGG+TGG - chr3_3:68649359-68649378 Msa0467380:intron 85.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 68648312 68649862 68648312 ID=Msa0467380;Name=Msa0467380
chr3_3 mRNA 68648312 68649862 68648312 ID=Msa0467380-mRNA-1;Parent=Msa0467380;Name=Msa0467380-mRNA-1;_AED=0.08;_eAED=0.08;_QI=121|0.5|0.8|1|1|1|5|432|148
chr3_3 exon 68648312 68648887 68648312 ID=Msa0467380-mRNA-1:exon:15983;Parent=Msa0467380-mRNA-1
chr3_3 exon 68648912 68648917 68648912 ID=Msa0467380-mRNA-1:exon:15982;Parent=Msa0467380-mRNA-1
chr3_3 exon 68648969 68649072 68648969 ID=Msa0467380-mRNA-1:exon:15981;Parent=Msa0467380-mRNA-1
chr3_3 exon 68649456 68649524 68649456 ID=Msa0467380-mRNA-1:exon:15980;Parent=Msa0467380-mRNA-1
chr3_3 exon 68649618 68649862 68649618 ID=Msa0467380-mRNA-1:exon:15979;Parent=Msa0467380-mRNA-1
chr3_3 five_prime_UTR 68649742 68649862 68649742 ID=Msa0467380-mRNA-1:five_prime_utr;Parent=Msa0467380-mRNA-1
chr3_3 CDS 68649618 68649741 68649618 ID=Msa0467380-mRNA-1:cds;Parent=Msa0467380-mRNA-1
chr3_3 CDS 68649456 68649524 68649456 ID=Msa0467380-mRNA-1:cds;Parent=Msa0467380-mRNA-1
chr3_3 CDS 68648969 68649072 68648969 ID=Msa0467380-mRNA-1:cds;Parent=Msa0467380-mRNA-1
chr3_3 CDS 68648912 68648917 68648912 ID=Msa0467380-mRNA-1:cds;Parent=Msa0467380-mRNA-1
chr3_3 CDS 68648744 68648887 68648744 ID=Msa0467380-mRNA-1:cds;Parent=Msa0467380-mRNA-1
chr3_3 three_prime_UTR 68648312 68648743 68648312 ID=Msa0467380-mRNA-1:three_prime_utr;Parent=Msa0467380-mRNA-1
Gene Sequence

>Msa0467380

ATGGCTTTCTGTAACAAGATTGGAAACCTCTTGAGGCAAGGTGCTACTCAGAGCACTCAAGCTCCTGTTTCATCCATGCTTAACTACTTGCGCCACATGTCTTCAAGCAAGCTTTTCATTGGAGGCCTTTCTTACCATGTTGATGACCAGTCTCTTAAGGATGCATTTACAACCTACGGAGAAGTGGTTGAGGCAAGAGTTATCACTGATAGAGACACTGGAAGATCAAGGGGATTTGGATTTGTCAACTTCACCAGTGAAGAGTCTGCAACTTCAGCACTCTCCATGGATGGACAGTCGGTTGATTTAAATGGGCGAAACATTCGTGTGTCATATGCAAATGATAGACAGGCTGGTGGACCTCGTTCCGAAGGTGGCTACAGCGGCGGCGGCGGTGGATACAGCGGCGGCGGCGGTGGATACGGCGGTGGTGGCGGCGGTTGGTGA
Protein sequence

>Msa0467380

MAFCNKIGNLLRQGATQSTQAPVSSMLNYLRHMSSSKLFIGGLSYHVDDQSLKDAFTTYGEVVEARVITDRDTGRSRGFGFVNFTSEESATSALSMDGQSVDLNGRNIRVSYANDRQAGGPRSEGGYSGGGGGYSGGGGGYGGGGGGW*