AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0358050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0358050 MtrunA17_Chr3g0115881 100.000 74 0 0 1 74 1 74 2.59e-49 149
Msa0358050 MtrunA17_Chr7g0214091 89.130 46 5 0 1 46 1 46 4.54e-24 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0358050 AT1G27435.1 72.000 75 17 1 3 73 6 80 8.74e-33 108

Find 35 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAGATGGGTGGTGCATTT+TGG 0.153987 3_1:+42782393 Msa0358050:intron
AGGTTGTTCCATCGGTATTT+TGG 0.238148 3_1:+42783187 Msa0358050:three_prime_UTR
CACACGGCGAAGACGCTTCT+TGG 0.328655 3_1:-42782507 None:intergenic
GTTGACCGGCTTCATAAAAT+AGG 0.333484 3_1:-42783460 None:intergenic
TGTAAGAAAGTTAAAATCAT+TGG 0.339565 3_1:-42783273 None:intergenic
GTGAAGAAGCATGATTCTTC+GGG 0.371640 3_1:+42782437 Msa0358050:CDS
AGTACTCTTTGTAACATTGT+TGG 0.406629 3_1:-42783141 None:intergenic
AGTGAAGAAGCATGATTCTT+CGG 0.422338 3_1:+42782436 Msa0358050:CDS
ATACCGATGGAACAACCTTC+AGG 0.424726 3_1:-42783182 None:intergenic
GCGAAGACGCTTCTTGGCAT+TGG 0.430692 3_1:-42782501 None:intergenic
ACTTGCTGAAGGAGCTGGTT+CGG 0.434113 3_1:-42783105 None:intergenic
GCGTCTTCGCCGTGTGTGGC+AGG 0.469382 3_1:+42782514 Msa0358050:CDS
ACAAAGAGTACTTAAGTTGA+AGG 0.470492 3_1:+42783152 Msa0358050:exon
GGCATTGGCTTTACGAGTTG+TGG 0.490914 3_1:-42782486 None:intergenic
CTTTCTTCGTCTCTTCCCAC+AGG 0.499169 3_1:+42781946 Msa0358050:five_prime_UTR
CTTTGTAACATTGTTGGCAC+TGG 0.504174 3_1:-42783135 None:intergenic
GAAGAACTTGCTGAAGGAGC+TGG 0.514090 3_1:-42783110 None:intergenic
CACATTATAGAACTGTTGAC+CGG 0.525045 3_1:-42783474 None:intergenic
TTGGGGTACGCGGGTGATGG+AGG 0.527082 3_1:+42782412 Msa0358050:CDS
CCACCTGAAGGTTGTTCCAT+CGG 0.559409 3_1:+42783179 Msa0358050:three_prime_UTR
GAAGAATGAAGCTGTCCTCA+AGG 0.567341 3_1:+42783076 Msa0358050:intron
CAAAGAGTACTTAAGTTGAA+GGG 0.582974 3_1:+42783153 Msa0358050:exon
GTTGAAGGGTGTCCACCTGA+AGG 0.585228 3_1:+42783167 Msa0358050:three_prime_UTR
GATGTGAATCGCTCAATCGC+GGG 0.589266 3_1:-42781913 None:intergenic
TGATGTGAATCGCTCAATCG+CGG 0.592200 3_1:-42781914 None:intergenic
AGAAGCGTCTTCGCCGTGTG+TGG 0.603774 3_1:+42782510 Msa0358050:CDS
GTGAATCGCTCAATCGCGGG+AGG 0.610710 3_1:-42781910 None:intergenic
TTGGGATCCCAAAATACCGA+TGG 0.617975 3_1:-42783195 None:intergenic
CTGGCAGAAGAACTTGCTGA+AGG 0.618337 3_1:-42783116 None:intergenic
ATTGAAATACCTGCCACACA+CGG 0.634795 3_1:-42782523 None:intergenic
TGAATCGCTCAATCGCGGGA+GGG 0.673908 3_1:-42781909 None:intergenic
TGAAGAAGCATGATTCTTCG+GGG 0.674050 3_1:+42782438 Msa0358050:CDS
CCGATGGAACAACCTTCAGG+TGG 0.677190 3_1:-42783179 None:intergenic
CTGGTTCGGAACAAGCCTTG+AGG 0.710113 3_1:-42783091 None:intergenic
GGTAAAATAAAATAGATGGG+TGG 0.724755 3_1:+42782383 Msa0358050:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATAAAAAAGTAAATTAA+AGG - chr3_1:42782016-42782035 None:intergenic 10.0%
!! GAAATAAAAAAGTAAATTAA+AGG - chr3_1:42782016-42782035 None:intergenic 10.0%
!!! CATTTTTCAGATAAAATTAA+AGG - chr3_1:42782038-42782057 None:intergenic 15.0%
!! AAGCATGTTATTAATTAAAT+AGG + chr3_1:42782596-42782615 Msa0358050:intron 15.0%
!!! CATTTTTCAGATAAAATTAA+AGG - chr3_1:42782038-42782057 None:intergenic 15.0%
!! AAGCATGTTATTAATTAAAT+AGG + chr3_1:42782596-42782615 Msa0358050:intron 15.0%
!!! AAGTTTTAATATATGTGTAG+CGG + chr3_1:42782121-42782140 Msa0358050:intron 20.0%
!!! AAACTATTAGTGAAACTTTT+AGG + chr3_1:42782813-42782832 Msa0358050:intron 20.0%
!! CACAATTATTGTTCAATATT+TGG - chr3_1:42783016-42783035 None:intergenic 20.0%
!!! TATGGTACATTTTTTGTTTT+TGG + chr3_1:42783054-42783073 Msa0358050:intron 20.0%
!! GCAAAAACTAATATAGAATT+GGG - chr3_1:42783216-42783235 None:intergenic 20.0%
!! TGTAAGAAAGTTAAAATCAT+TGG - chr3_1:42783276-42783295 None:intergenic 20.0%
!!! TGTAAGTTGTAAACTTTTTT+AGG + chr3_1:42783306-42783325 Msa0358050:three_prime_UTR 20.0%
!!! GTAAGTTGTAAACTTTTTTA+GGG + chr3_1:42783307-42783326 Msa0358050:three_prime_UTR 20.0%
!!! AAGTTTTAATATATGTGTAG+CGG + chr3_1:42782121-42782140 Msa0358050:intron 20.0%
!!! AAACTATTAGTGAAACTTTT+AGG + chr3_1:42782813-42782832 Msa0358050:intron 20.0%
!! CACAATTATTGTTCAATATT+TGG - chr3_1:42783016-42783035 None:intergenic 20.0%
!!! TATGGTACATTTTTTGTTTT+TGG + chr3_1:42783054-42783073 Msa0358050:intron 20.0%
!! GCAAAAACTAATATAGAATT+GGG - chr3_1:42783216-42783235 None:intergenic 20.0%
!! TGTAAGAAAGTTAAAATCAT+TGG - chr3_1:42783276-42783295 None:intergenic 20.0%
!!! TGTAAGTTGTAAACTTTTTT+AGG + chr3_1:42783306-42783325 Msa0358050:three_prime_UTR 20.0%
!!! GTAAGTTGTAAACTTTTTTA+GGG + chr3_1:42783307-42783326 Msa0358050:three_prime_UTR 20.0%
! GATTGTGATTTATGGTATTA+TGG + chr3_1:42782063-42782082 Msa0358050:intron 25.0%
!!! TCCTAAGTTTTTTACATTAG+CGG + chr3_1:42782150-42782169 Msa0358050:intron 25.0%
! TAGTAATAATCTATGAAGCA+CGG - chr3_1:42782219-42782238 None:intergenic 25.0%
! TGCTGGTAAAATAAAATAGA+TGG + chr3_1:42782379-42782398 Msa0358050:intron 25.0%
! GCTGGTAAAATAAAATAGAT+GGG + chr3_1:42782380-42782399 Msa0358050:intron 25.0%
! ATACCTAGAAGAAATGATTA+AGG + chr3_1:42782625-42782644 Msa0358050:intron 25.0%
!!! ATTTATTGTTTTATCGTCTG+TGG + chr3_1:42782654-42782673 Msa0358050:intron 25.0%
!! GTTTCACTAATAGTTTTTCT+AGG - chr3_1:42782809-42782828 None:intergenic 25.0%
!!! TGAAACTTTTAGGAAAAACT+TGG + chr3_1:42782823-42782842 Msa0358050:intron 25.0%
! TATGGTTTGAGTAAAGATTA+TGG + chr3_1:42782868-42782887 Msa0358050:intron 25.0%
! GGCAAAAACTAATATAGAAT+TGG - chr3_1:42783217-42783236 None:intergenic 25.0%
! GATTGTGATTTATGGTATTA+TGG + chr3_1:42782063-42782082 Msa0358050:intron 25.0%
!!! TCCTAAGTTTTTTACATTAG+CGG + chr3_1:42782150-42782169 Msa0358050:intron 25.0%
! TAGTAATAATCTATGAAGCA+CGG - chr3_1:42782219-42782238 None:intergenic 25.0%
! TGCTGGTAAAATAAAATAGA+TGG + chr3_1:42782379-42782398 Msa0358050:intron 25.0%
! GCTGGTAAAATAAAATAGAT+GGG + chr3_1:42782380-42782399 Msa0358050:intron 25.0%
! ATACCTAGAAGAAATGATTA+AGG + chr3_1:42782625-42782644 Msa0358050:intron 25.0%
!!! ATTTATTGTTTTATCGTCTG+TGG + chr3_1:42782654-42782673 Msa0358050:intron 25.0%
!! GTTTCACTAATAGTTTTTCT+AGG - chr3_1:42782809-42782828 None:intergenic 25.0%
!!! TGAAACTTTTAGGAAAAACT+TGG + chr3_1:42782823-42782842 Msa0358050:intron 25.0%
! TATGGTTTGAGTAAAGATTA+TGG + chr3_1:42782868-42782887 Msa0358050:intron 25.0%
! GGCAAAAACTAATATAGAAT+TGG - chr3_1:42783217-42783236 None:intergenic 25.0%
ATGTTGCAGATTGTGATTTA+TGG + chr3_1:42782055-42782074 Msa0358050:intron 30.0%
ACCGCTAATGTAAAAAACTT+AGG - chr3_1:42782154-42782173 None:intergenic 30.0%
!!! TTGAATCTGATTTGTTTTGC+TGG + chr3_1:42782362-42782381 Msa0358050:intron 30.0%
GGTAAAATAAAATAGATGGG+TGG + chr3_1:42782383-42782402 Msa0358050:intron 30.0%
CATCCTTAATCATTTCTTCT+AGG - chr3_1:42782631-42782650 None:intergenic 30.0%
TGATGAGCATTTAGTAAAGA+TGG + chr3_1:42782683-42782702 Msa0358050:intron 30.0%
TCTACTAGTACAAAATCTAC+AGG - chr3_1:42782746-42782765 None:intergenic 30.0%
! TTTTGTACTAGTAGATCAGA+TGG + chr3_1:42782751-42782770 Msa0358050:intron 30.0%
AAACTTGGAGATTAATGAGT+TGG + chr3_1:42782838-42782857 Msa0358050:intron 30.0%
TCTTTACTCAAACCATATCT+TGG - chr3_1:42782865-42782884 None:intergenic 30.0%
ACAATAATTGTGACTGTCAA+TGG + chr3_1:42783024-42783043 Msa0358050:intron 30.0%
AGTACTCTTTGTAACATTGT+TGG - chr3_1:42783144-42783163 None:intergenic 30.0%
!! ACAAAGAGTACTTAAGTTGA+AGG + chr3_1:42783152-42783171 Msa0358050:exon 30.0%
!! CAAAGAGTACTTAAGTTGAA+GGG + chr3_1:42783153-42783172 Msa0358050:exon 30.0%
!! TCTACAGTTTTTCAAATGTG+TGG - chr3_1:42783238-42783257 None:intergenic 30.0%
ACACATTTGAAAAACTGTAG+AGG + chr3_1:42783237-42783256 Msa0358050:three_prime_UTR 30.0%
ATGTTGCAGATTGTGATTTA+TGG + chr3_1:42782055-42782074 Msa0358050:intron 30.0%
ACCGCTAATGTAAAAAACTT+AGG - chr3_1:42782154-42782173 None:intergenic 30.0%
!!! TTGAATCTGATTTGTTTTGC+TGG + chr3_1:42782362-42782381 Msa0358050:intron 30.0%
GGTAAAATAAAATAGATGGG+TGG + chr3_1:42782383-42782402 Msa0358050:intron 30.0%
CATCCTTAATCATTTCTTCT+AGG - chr3_1:42782631-42782650 None:intergenic 30.0%
TGATGAGCATTTAGTAAAGA+TGG + chr3_1:42782683-42782702 Msa0358050:intron 30.0%
TCTACTAGTACAAAATCTAC+AGG - chr3_1:42782746-42782765 None:intergenic 30.0%
! TTTTGTACTAGTAGATCAGA+TGG + chr3_1:42782751-42782770 Msa0358050:intron 30.0%
AAACTTGGAGATTAATGAGT+TGG + chr3_1:42782838-42782857 Msa0358050:intron 30.0%
TCTTTACTCAAACCATATCT+TGG - chr3_1:42782865-42782884 None:intergenic 30.0%
ACAATAATTGTGACTGTCAA+TGG + chr3_1:42783024-42783043 Msa0358050:intron 30.0%
AGTACTCTTTGTAACATTGT+TGG - chr3_1:42783144-42783163 None:intergenic 30.0%
!! ACAAAGAGTACTTAAGTTGA+AGG + chr3_1:42783152-42783171 Msa0358050:exon 30.0%
!! CAAAGAGTACTTAAGTTGAA+GGG + chr3_1:42783153-42783172 Msa0358050:exon 30.0%
!! TCTACAGTTTTTCAAATGTG+TGG - chr3_1:42783238-42783257 None:intergenic 30.0%
ACACATTTGAAAAACTGTAG+AGG + chr3_1:42783237-42783256 Msa0358050:three_prime_UTR 30.0%
ACAAATTGCTTCTGACTACT+GGG + chr3_1:42782312-42782331 Msa0358050:intron 35.0%
! AGTGAAGAAGCATGATTCTT+CGG + chr3_1:42782436-42782455 Msa0358050:CDS 35.0%
TGAGCATTTAGTAAAGATGG+TGG + chr3_1:42782686-42782705 Msa0358050:intron 35.0%
!! AAGATGGTGGAAAATAAGCT+TGG + chr3_1:42782699-42782718 Msa0358050:intron 35.0%
GTGGAAAATAAGCTTGGTTT+GGG + chr3_1:42782705-42782724 Msa0358050:intron 35.0%
! TAATGAGTTGGACCAAGATA+TGG + chr3_1:42782850-42782869 Msa0358050:intron 35.0%
ACTGTCAATGGCTAATGTTA+TGG + chr3_1:42783036-42783055 Msa0358050:intron 35.0%
! TCTTGCCTATTTTATGAAGC+CGG + chr3_1:42783455-42783474 Msa0358050:three_prime_UTR 35.0%
ACAAATTGCTTCTGACTACT+GGG + chr3_1:42782312-42782331 Msa0358050:intron 35.0%
! AGTGAAGAAGCATGATTCTT+CGG + chr3_1:42782436-42782455 Msa0358050:CDS 35.0%
TGAGCATTTAGTAAAGATGG+TGG + chr3_1:42782686-42782705 Msa0358050:intron 35.0%
!! AAGATGGTGGAAAATAAGCT+TGG + chr3_1:42782699-42782718 Msa0358050:intron 35.0%
GTGGAAAATAAGCTTGGTTT+GGG + chr3_1:42782705-42782724 Msa0358050:intron 35.0%
! TAATGAGTTGGACCAAGATA+TGG + chr3_1:42782850-42782869 Msa0358050:intron 35.0%
ACTGTCAATGGCTAATGTTA+TGG + chr3_1:42783036-42783055 Msa0358050:intron 35.0%
! TCTTGCCTATTTTATGAAGC+CGG + chr3_1:42783455-42783474 Msa0358050:three_prime_UTR 35.0%
!!! GTTTTAGGGTTTAGTTGAGG+AGG - chr3_1:42781890-42781909 None:intergenic 40.0%
!!! AGGGTTTTAGGGTTTAGTTG+AGG - chr3_1:42781893-42781912 None:intergenic 40.0%
GAGAGAAGAAAGAAACCTGT+GGG - chr3_1:42781964-42781983 None:intergenic 40.0%
AGAGAGAAGAAAGAAACCTG+TGG - chr3_1:42781965-42781984 None:intergenic 40.0%
!! TTTTTACATTAGCGGTGTCG+AGG + chr3_1:42782158-42782177 Msa0358050:intron 40.0%
GAAGCAATTTGTGATCAAGC+TGG - chr3_1:42782304-42782323 None:intergenic 40.0%
CACAAATTGCTTCTGACTAC+TGG + chr3_1:42782311-42782330 Msa0358050:intron 40.0%
! AATAGATGGGTGGTGCATTT+TGG + chr3_1:42782393-42782412 Msa0358050:intron 40.0%
! ATAGATGGGTGGTGCATTTT+GGG + chr3_1:42782394-42782413 Msa0358050:intron 40.0%
! GTGAAGAAGCATGATTCTTC+GGG + chr3_1:42782437-42782456 Msa0358050:CDS 40.0%
! TGAAGAAGCATGATTCTTCG+GGG + chr3_1:42782438-42782457 Msa0358050:CDS 40.0%
! ATGATTCTTCGGGGCTTTTA+TGG + chr3_1:42782447-42782466 Msa0358050:CDS 40.0%
ATTGAAATACCTGCCACACA+CGG - chr3_1:42782526-42782545 None:intergenic 40.0%
GGTGGAAAATAAGCTTGGTT+TGG + chr3_1:42782704-42782723 Msa0358050:intron 40.0%
CTAGTAGATCAGATGGAGAA+TGG + chr3_1:42782758-42782777 Msa0358050:intron 40.0%
TAGATCAGATGGAGAATGGT+CGG + chr3_1:42782762-42782781 Msa0358050:intron 40.0%
CTTTGTAACATTGTTGGCAC+TGG - chr3_1:42783138-42783157 None:intergenic 40.0%
! AGGTTGTTCCATCGGTATTT+TGG + chr3_1:42783187-42783206 Msa0358050:three_prime_UTR 40.0%
! GGTTGTTCCATCGGTATTTT+GGG + chr3_1:42783188-42783207 Msa0358050:three_prime_UTR 40.0%
! GTTGACCGGCTTCATAAAAT+AGG - chr3_1:42783463-42783482 None:intergenic 40.0%
!!! GTTTTAGGGTTTAGTTGAGG+AGG - chr3_1:42781890-42781909 None:intergenic 40.0%
!!! AGGGTTTTAGGGTTTAGTTG+AGG - chr3_1:42781893-42781912 None:intergenic 40.0%
GAGAGAAGAAAGAAACCTGT+GGG - chr3_1:42781964-42781983 None:intergenic 40.0%
AGAGAGAAGAAAGAAACCTG+TGG - chr3_1:42781965-42781984 None:intergenic 40.0%
!! TTTTTACATTAGCGGTGTCG+AGG + chr3_1:42782158-42782177 Msa0358050:intron 40.0%
GAAGCAATTTGTGATCAAGC+TGG - chr3_1:42782304-42782323 None:intergenic 40.0%
CACAAATTGCTTCTGACTAC+TGG + chr3_1:42782311-42782330 Msa0358050:intron 40.0%
! AATAGATGGGTGGTGCATTT+TGG + chr3_1:42782393-42782412 Msa0358050:intron 40.0%
! ATAGATGGGTGGTGCATTTT+GGG + chr3_1:42782394-42782413 Msa0358050:intron 40.0%
! GTGAAGAAGCATGATTCTTC+GGG + chr3_1:42782437-42782456 Msa0358050:CDS 40.0%
! TGAAGAAGCATGATTCTTCG+GGG + chr3_1:42782438-42782457 Msa0358050:CDS 40.0%
! ATGATTCTTCGGGGCTTTTA+TGG + chr3_1:42782447-42782466 Msa0358050:CDS 40.0%
ATTGAAATACCTGCCACACA+CGG - chr3_1:42782526-42782545 None:intergenic 40.0%
GGTGGAAAATAAGCTTGGTT+TGG + chr3_1:42782704-42782723 Msa0358050:intron 40.0%
CTAGTAGATCAGATGGAGAA+TGG + chr3_1:42782758-42782777 Msa0358050:intron 40.0%
TAGATCAGATGGAGAATGGT+CGG + chr3_1:42782762-42782781 Msa0358050:intron 40.0%
CTTTGTAACATTGTTGGCAC+TGG - chr3_1:42783138-42783157 None:intergenic 40.0%
! AGGTTGTTCCATCGGTATTT+TGG + chr3_1:42783187-42783206 Msa0358050:three_prime_UTR 40.0%
! GGTTGTTCCATCGGTATTTT+GGG + chr3_1:42783188-42783207 Msa0358050:three_prime_UTR 40.0%
! GTTGACCGGCTTCATAAAAT+AGG - chr3_1:42783463-42783482 None:intergenic 40.0%
TGATGTGAATCGCTCAATCG+CGG - chr3_1:42781917-42781936 None:intergenic 45.0%
GCAATTTGTGATCAAGCTGG+CGG - chr3_1:42782301-42782320 None:intergenic 45.0%
! TAGATGGGTGGTGCATTTTG+GGG + chr3_1:42782395-42782414 Msa0358050:intron 45.0%
AAGCCCTATCTCACTAAGTG+GGG - chr3_1:42782917-42782936 None:intergenic 45.0%
CAAGCCCTATCTCACTAAGT+GGG - chr3_1:42782918-42782937 None:intergenic 45.0%
GAAGAATGAAGCTGTCCTCA+AGG + chr3_1:42783076-42783095 Msa0358050:intron 45.0%
ATACCGATGGAACAACCTTC+AGG - chr3_1:42783185-42783204 None:intergenic 45.0%
TTGGGATCCCAAAATACCGA+TGG - chr3_1:42783198-42783217 None:intergenic 45.0%
TGATGTGAATCGCTCAATCG+CGG - chr3_1:42781917-42781936 None:intergenic 45.0%
GCAATTTGTGATCAAGCTGG+CGG - chr3_1:42782301-42782320 None:intergenic 45.0%
! TAGATGGGTGGTGCATTTTG+GGG + chr3_1:42782395-42782414 Msa0358050:intron 45.0%
AAGCCCTATCTCACTAAGTG+GGG - chr3_1:42782917-42782936 None:intergenic 45.0%
CAAGCCCTATCTCACTAAGT+GGG - chr3_1:42782918-42782937 None:intergenic 45.0%
GAAGAATGAAGCTGTCCTCA+AGG + chr3_1:42783076-42783095 Msa0358050:intron 45.0%
ATACCGATGGAACAACCTTC+AGG - chr3_1:42783185-42783204 None:intergenic 45.0%
TTGGGATCCCAAAATACCGA+TGG - chr3_1:42783198-42783217 None:intergenic 45.0%
! TCAATCGCGGGAGGGTTTTA+GGG - chr3_1:42781904-42781923 None:intergenic 50.0%
GATGTGAATCGCTCAATCGC+GGG - chr3_1:42781916-42781935 None:intergenic 50.0%
CTTTCTTCGTCTCTTCCCAC+AGG + chr3_1:42781946-42781965 Msa0358050:five_prime_UTR 50.0%
ATGAAGCACGGACTCGAACA+CGG - chr3_1:42782207-42782226 None:intergenic 50.0%
!! GGCATTGGCTTTACGAGTTG+TGG - chr3_1:42782489-42782508 None:intergenic 50.0%
GGTTTGGGCATGTAGAGTGA+AGG + chr3_1:42782720-42782739 Msa0358050:intron 50.0%
GAGAATGGTCGGATCACTAG+AGG + chr3_1:42782773-42782792 Msa0358050:intron 50.0%
CCTATCTCACTAAGTGGGGT+CGG - chr3_1:42782913-42782932 None:intergenic 50.0%
CGACCCCACTTAGTGAGATA+GGG + chr3_1:42782911-42782930 Msa0358050:intron 50.0%
CCAAGCCCTATCTCACTAAG+TGG - chr3_1:42782919-42782938 None:intergenic 50.0%
CCACTTAGTGAGATAGGGCT+TGG + chr3_1:42782916-42782935 Msa0358050:intron 50.0%
! ACTTGCTGAAGGAGCTGGTT+CGG - chr3_1:42783108-42783127 None:intergenic 50.0%
!! GAAGAACTTGCTGAAGGAGC+TGG - chr3_1:42783113-42783132 None:intergenic 50.0%
!! CTGGCAGAAGAACTTGCTGA+AGG - chr3_1:42783119-42783138 None:intergenic 50.0%
CCACCTGAAGGTTGTTCCAT+CGG + chr3_1:42783179-42783198 Msa0358050:three_prime_UTR 50.0%
! TCAATCGCGGGAGGGTTTTA+GGG - chr3_1:42781904-42781923 None:intergenic 50.0%
GATGTGAATCGCTCAATCGC+GGG - chr3_1:42781916-42781935 None:intergenic 50.0%
CTTTCTTCGTCTCTTCCCAC+AGG + chr3_1:42781946-42781965 Msa0358050:five_prime_UTR 50.0%
ATGAAGCACGGACTCGAACA+CGG - chr3_1:42782207-42782226 None:intergenic 50.0%
!! GGCATTGGCTTTACGAGTTG+TGG - chr3_1:42782489-42782508 None:intergenic 50.0%
GGTTTGGGCATGTAGAGTGA+AGG + chr3_1:42782720-42782739 Msa0358050:intron 50.0%
GAGAATGGTCGGATCACTAG+AGG + chr3_1:42782773-42782792 Msa0358050:intron 50.0%
CCTATCTCACTAAGTGGGGT+CGG - chr3_1:42782913-42782932 None:intergenic 50.0%
CGACCCCACTTAGTGAGATA+GGG + chr3_1:42782911-42782930 Msa0358050:intron 50.0%
CCAAGCCCTATCTCACTAAG+TGG - chr3_1:42782919-42782938 None:intergenic 50.0%
CCACTTAGTGAGATAGGGCT+TGG + chr3_1:42782916-42782935 Msa0358050:intron 50.0%
! ACTTGCTGAAGGAGCTGGTT+CGG - chr3_1:42783108-42783127 None:intergenic 50.0%
!! GAAGAACTTGCTGAAGGAGC+TGG - chr3_1:42783113-42783132 None:intergenic 50.0%
!! CTGGCAGAAGAACTTGCTGA+AGG - chr3_1:42783119-42783138 None:intergenic 50.0%
CCACCTGAAGGTTGTTCCAT+CGG + chr3_1:42783179-42783198 Msa0358050:three_prime_UTR 50.0%
! CTCAATCGCGGGAGGGTTTT+AGG - chr3_1:42781905-42781924 None:intergenic 55.0%
TGAATCGCTCAATCGCGGGA+GGG - chr3_1:42781912-42781931 None:intergenic 55.0%
AGGTGTCTGTGTGTCGTGTC+AGG + chr3_1:42782178-42782197 Msa0358050:intron 55.0%
!!! GTGGTGCATTTTGGGGTACG+CGG + chr3_1:42782402-42782421 Msa0358050:CDS 55.0%
!!! TGGTGCATTTTGGGGTACGC+GGG + chr3_1:42782403-42782422 Msa0358050:CDS 55.0%
!!! ATTTTGGGGTACGCGGGTGA+TGG + chr3_1:42782409-42782428 Msa0358050:CDS 55.0%
GCGAAGACGCTTCTTGGCAT+TGG - chr3_1:42782504-42782523 None:intergenic 55.0%
GGTCGGATCACTAGAGGAAG+AGG + chr3_1:42782779-42782798 Msa0358050:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG - chr3_1:42782905-42782924 None:intergenic 55.0%
CCGACCCCACTTAGTGAGAT+AGG + chr3_1:42782910-42782929 Msa0358050:intron 55.0%
!! CTGGTTCGGAACAAGCCTTG+AGG - chr3_1:42783094-42783113 None:intergenic 55.0%
!! GTTGAAGGGTGTCCACCTGA+AGG + chr3_1:42783167-42783186 Msa0358050:three_prime_UTR 55.0%
CCGATGGAACAACCTTCAGG+TGG - chr3_1:42783182-42783201 None:intergenic 55.0%
! CTCAATCGCGGGAGGGTTTT+AGG - chr3_1:42781905-42781924 None:intergenic 55.0%
TGAATCGCTCAATCGCGGGA+GGG - chr3_1:42781912-42781931 None:intergenic 55.0%
AGGTGTCTGTGTGTCGTGTC+AGG + chr3_1:42782178-42782197 Msa0358050:intron 55.0%
!!! GTGGTGCATTTTGGGGTACG+CGG + chr3_1:42782402-42782421 Msa0358050:CDS 55.0%
!!! TGGTGCATTTTGGGGTACGC+GGG + chr3_1:42782403-42782422 Msa0358050:CDS 55.0%
!!! ATTTTGGGGTACGCGGGTGA+TGG + chr3_1:42782409-42782428 Msa0358050:CDS 55.0%
GCGAAGACGCTTCTTGGCAT+TGG - chr3_1:42782504-42782523 None:intergenic 55.0%
GGTCGGATCACTAGAGGAAG+AGG + chr3_1:42782779-42782798 Msa0358050:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG - chr3_1:42782905-42782924 None:intergenic 55.0%
CCGACCCCACTTAGTGAGAT+AGG + chr3_1:42782910-42782929 Msa0358050:intron 55.0%
!! CTGGTTCGGAACAAGCCTTG+AGG - chr3_1:42783094-42783113 None:intergenic 55.0%
!! GTTGAAGGGTGTCCACCTGA+AGG + chr3_1:42783167-42783186 Msa0358050:three_prime_UTR 55.0%
CCGATGGAACAACCTTCAGG+TGG - chr3_1:42783182-42783201 None:intergenic 55.0%
GTGAATCGCTCAATCGCGGG+AGG - chr3_1:42781913-42781932 None:intergenic 60.0%
CACACGGCGAAGACGCTTCT+TGG - chr3_1:42782510-42782529 None:intergenic 60.0%
!! AGAAGCGTCTTCGCCGTGTG+TGG + chr3_1:42782510-42782529 Msa0358050:CDS 60.0%
GTGAATCGCTCAATCGCGGG+AGG - chr3_1:42781913-42781932 None:intergenic 60.0%
CACACGGCGAAGACGCTTCT+TGG - chr3_1:42782510-42782529 None:intergenic 60.0%
!! AGAAGCGTCTTCGCCGTGTG+TGG + chr3_1:42782510-42782529 Msa0358050:CDS 60.0%
! TTGGGGTACGCGGGTGATGG+AGG + chr3_1:42782412-42782431 Msa0358050:CDS 65.0%
! TTGGGGTACGCGGGTGATGG+AGG + chr3_1:42782412-42782431 Msa0358050:CDS 65.0%
GCGTCTTCGCCGTGTGTGGC+AGG + chr3_1:42782514-42782533 Msa0358050:CDS 70.0%
GCGTCTTCGCCGTGTGTGGC+AGG + chr3_1:42782514-42782533 Msa0358050:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 42781867 42783494 42781867 ID=Msa0358050;Name=Msa0358050
chr3_1 mRNA 42781867 42783494 42781867 ID=Msa0358050-mRNA-1;Parent=Msa0358050;Name=Msa0358050-mRNA-1;_AED=0.02;_eAED=0.02;_QI=101|1|1|1|0.5|0.66|3|328|74
chr3_1 exon 42781867 42781967 42781867 ID=Msa0358050-mRNA-1:exon:7326;Parent=Msa0358050-mRNA-1
chr3_1 exon 42782398 42782535 42782398 ID=Msa0358050-mRNA-1:exon:7327;Parent=Msa0358050-mRNA-1
chr3_1 exon 42783080 42783494 42783080 ID=Msa0358050-mRNA-1:exon:7328;Parent=Msa0358050-mRNA-1
chr3_1 five_prime_UTR 42781867 42781967 42781867 ID=Msa0358050-mRNA-1:five_prime_utr;Parent=Msa0358050-mRNA-1
chr3_1 CDS 42782398 42782535 42782398 ID=Msa0358050-mRNA-1:cds;Parent=Msa0358050-mRNA-1
chr3_1 CDS 42783080 42783166 42783080 ID=Msa0358050-mRNA-1:cds;Parent=Msa0358050-mRNA-1
chr3_1 three_prime_UTR 42783167 42783494 42783167 ID=Msa0358050-mRNA-1:three_prime_utr;Parent=Msa0358050-mRNA-1
Gene Sequence

>Msa0358050

ATGGGTGGTGCATTTTGGGGTACGCGGGTGATGGAGGTAGTGAAGAAGCATGATTCTTCGGGGCTTTTATGGAAGCGAATCAAACTCACCACAACTCGTAAAGCCAATGCCAAGAAGCGTCTTCGCCGTGTGTGGCAGAATGAAGCTGTCCTCAAGGCTTGTTCCGAACCAGCTCCTTCAGCAAGTTCTTCTGCCAGTGCCAACAATGTTACAAAGAGTACTTAA
Protein sequence

>Msa0358050

MGGAFWGTRVMEVVKKHDSSGLLWKRIKLTTTRKANAKKRLRRVWQNEAVLKACSEPAPSASSSASANNVTKST*