AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0469830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469830 MtrunA17_Chr3g0124671 93.182 176 10 1 1 174 1 176 1.79e-109 309
Msa0469830 MtrunA17_Chr8g0347721 51.852 135 50 4 30 164 2 121 2.81e-33 114
Msa0469830 MtrunA17_Chr8g0384901 45.985 137 58 6 32 162 24 150 8.17e-25 94.0
Msa0469830 MtrunA17_Chr1g0159961 66.176 68 15 3 1 67 1 61 4.96e-18 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469830 AT4G08330.1 61.654 133 46 2 31 163 33 160 1.09e-51 163
Msa0469830 AT4G08330.2 58.824 119 44 2 45 163 36 149 6.02e-42 138

Find 45 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCGATAGATTCAAAATA+TGG 0.268899 3_3:-71826697 Msa0469830:CDS
TCCCAACATCTTTGTTGCTT+TGG 0.288213 3_3:+71826230 None:intergenic
CGCAAGTGTTGCAACCATAT+TGG 0.297071 3_3:-71826535 Msa0469830:CDS
ATATCCCATTTCCACATTCT+TGG 0.326262 3_3:+71826371 None:intergenic
CCTCTTCTTCTACTACTTCT+CGG 0.327764 3_3:-71827683 Msa0469830:CDS
CATTGTAAGCATAACCAATA+TGG 0.355065 3_3:+71826521 None:intergenic
TCTTCCCAAGAATGTGGAAA+TGG 0.359240 3_3:-71826376 Msa0469830:CDS
TATCCCATTTCCACATTCTT+GGG 0.383630 3_3:+71826372 None:intergenic
ATGCACTATCACACAAATAT+AGG 0.394685 3_3:+71826293 None:intergenic
AATCTATCGATGATGTGTTT+CGG 0.412775 3_3:+71826707 None:intergenic
ACCAAAGCAACAAAGATGTT+GGG 0.418636 3_3:-71826231 Msa0469830:three_prime_UTR
CCACATTCTTGGGAAGATGA+TGG 0.443507 3_3:+71826382 None:intergenic
TACCAAAGCAACAAAGATGT+TGG 0.448057 3_3:-71826232 Msa0469830:three_prime_UTR
CGAGACGGTGCTTCAGTTGA+AGG 0.452110 3_3:+71826439 None:intergenic
CTTCCCAAGAATGTGGAAAT+GGG 0.463725 3_3:-71826375 Msa0469830:CDS
TCGTCAACATTGATGATAGC+AGG 0.465053 3_3:-71826645 Msa0469830:CDS
AAGAAGAGGAGGATGACAAA+GGG 0.470495 3_3:+71827697 None:intergenic
TTTATTCTAGTATCATCAAA+TGG 0.485734 3_3:-71826475 Msa0469830:CDS
CTTCTCGGACTCGGAGTCAA+AGG 0.487065 3_3:-71827668 Msa0469830:CDS
GGTTTGATGAGGATAGGTTA+AGG 0.497317 3_3:+71826728 None:intergenic
GTCAAAGGGATGTCCGCTAC+AGG 0.503245 3_3:-71827653 Msa0469830:intron
GAAGAAGAGGAGGATGACAA+AGG 0.510515 3_3:+71827696 None:intergenic
TGATGATAGCAGGTTTGCAC+AGG 0.514951 3_3:-71826635 Msa0469830:CDS
TAATACTCACCAGCAACAAA+AGG 0.521848 3_3:-71826265 Msa0469830:three_prime_UTR
TTCTCGGACTCGGAGTCAAA+GGG 0.527617 3_3:-71827667 Msa0469830:CDS
CCGAGAAGTAGTAGAAGAAG+AGG 0.529630 3_3:+71827683 None:intergenic
CTTCTACTACTTCTCGGACT+CGG 0.532316 3_3:-71827677 Msa0469830:CDS
GTAAATTATTACTATAGAGA+AGG 0.541974 3_3:+71827814 None:intergenic
GAAAATTTACATACATATTG+TGG 0.543436 3_3:-71826336 Msa0469830:three_prime_UTR
AGAAGTAGTAGAAGAAGAGG+AGG 0.549787 3_3:+71827686 None:intergenic
TGGAAATGGGATATCTGTCT+TGG 0.550576 3_3:-71826362 Msa0469830:CDS
CATATTGGTTATGCTTACAA+TGG 0.558093 3_3:-71826520 Msa0469830:CDS
TTATTACTATAGAGAAGGGT+GGG 0.560831 3_3:+71827819 None:intergenic
GGTGCTTCAGTTGAAGGAGA+TGG 0.568432 3_3:+71826445 None:intergenic
ATTATTACTATAGAGAAGGG+TGG 0.571021 3_3:+71827818 None:intergenic
TGTTTCGGTTTGATGAGGAT+AGG 0.584347 3_3:+71826722 None:intergenic
TAAATTATTACTATAGAGAA+GGG 0.594034 3_3:+71827815 None:intergenic
CCATCATCTTCCCAAGAATG+TGG 0.600174 3_3:-71826382 Msa0469830:CDS
TTGGGAAGATGATGGCTGCA+AGG 0.606294 3_3:+71826390 None:intergenic
TGATGTGTTTCGGTTTGATG+AGG 0.619348 3_3:+71826717 None:intergenic
GATGATACTAGAATAAATGA+TGG 0.622976 3_3:+71826481 None:intergenic
AGAGAGTGGTGAACCTGTAG+CGG 0.624601 3_3:+71827640 None:intergenic
ATGTTGTATTTCACACGAGA+CGG 0.639297 3_3:+71826424 None:intergenic
TTACTATAGAGAAGGGTGGG+AGG 0.657772 3_3:+71827822 None:intergenic
TATGGAAAATCAATAAAACG+AGG 0.753759 3_3:-71826679 Msa0469830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAGTTTAAGAAAATTA+GGG + chr3_3:71826698-71826717 None:intergenic 10.0%
!! TAATAAGTTTAAGAAAATTA+GGG + chr3_3:71826698-71826717 None:intergenic 10.0%
!! TAAATTATTACTATAGAGAA+GGG + chr3_3:71826194-71826213 None:intergenic 15.0%
!! TGTATAAACATAAATAGAAA+TGG + chr3_3:71826409-71826428 None:intergenic 15.0%
!! CTAATAAGTTTAAGAAAATT+AGG + chr3_3:71826699-71826718 None:intergenic 15.0%
!!! AGAATTTTAAATGTATGATA+AGG + chr3_3:71827061-71827080 None:intergenic 15.0%
!!! TACATTTAAAATTCTTGTTT+TGG - chr3_3:71827066-71827085 Msa0469830:intron 15.0%
!! TAAATTATTACTATAGAGAA+GGG + chr3_3:71826194-71826213 None:intergenic 15.0%
!! TGTATAAACATAAATAGAAA+TGG + chr3_3:71826409-71826428 None:intergenic 15.0%
!! CTAATAAGTTTAAGAAAATT+AGG + chr3_3:71826699-71826718 None:intergenic 15.0%
!!! AGAATTTTAAATGTATGATA+AGG + chr3_3:71827061-71827080 None:intergenic 15.0%
!!! TACATTTAAAATTCTTGTTT+TGG - chr3_3:71827066-71827085 Msa0469830:intron 15.0%
!! GTAAATTATTACTATAGAGA+AGG + chr3_3:71826195-71826214 None:intergenic 20.0%
!!! TTCAATAATTAATTTTGCCT+TGG - chr3_3:71826442-71826461 Msa0469830:CDS 20.0%
!!! GATTTTTTATACTTTCATAG+AGG - chr3_3:71826547-71826566 Msa0469830:CDS 20.0%
!!! ATTGCAATAATTTTGACAAT+AGG + chr3_3:71826617-71826636 None:intergenic 20.0%
!!! AAACATTTTTCATTTTGCTT+AGG - chr3_3:71826847-71826866 Msa0469830:intron 20.0%
!! CTAGAAATTAATTGAAATGA+AGG - chr3_3:71826991-71827010 Msa0469830:intron 20.0%
!! AATTAATTGAAATGAAGGAA+AGG - chr3_3:71826996-71827015 Msa0469830:intron 20.0%
!! ATTAATTGAAATGAAGGAAA+GGG - chr3_3:71826997-71827016 Msa0469830:intron 20.0%
!! TGAAATATGTAAATCATGAT+CGG + chr3_3:71827207-71827226 None:intergenic 20.0%
!!! TCATTTTTCTAAGAATTTGT+GGG - chr3_3:71827416-71827435 Msa0469830:intron 20.0%
!! TTTATTCTAGTATCATCAAA+TGG - chr3_3:71827531-71827550 Msa0469830:intron 20.0%
!! GAAAATTTACATACATATTG+TGG - chr3_3:71827670-71827689 Msa0469830:CDS 20.0%
!! GTAAATTATTACTATAGAGA+AGG + chr3_3:71826195-71826214 None:intergenic 20.0%
!!! TTCAATAATTAATTTTGCCT+TGG - chr3_3:71826442-71826461 Msa0469830:CDS 20.0%
!!! GATTTTTTATACTTTCATAG+AGG - chr3_3:71826547-71826566 Msa0469830:CDS 20.0%
!!! ATTGCAATAATTTTGACAAT+AGG + chr3_3:71826617-71826636 None:intergenic 20.0%
!!! AAACATTTTTCATTTTGCTT+AGG - chr3_3:71826847-71826866 Msa0469830:intron 20.0%
!! CTAGAAATTAATTGAAATGA+AGG - chr3_3:71826991-71827010 Msa0469830:intron 20.0%
!! AATTAATTGAAATGAAGGAA+AGG - chr3_3:71826996-71827015 Msa0469830:intron 20.0%
!! ATTAATTGAAATGAAGGAAA+GGG - chr3_3:71826997-71827016 Msa0469830:intron 20.0%
!! TGAAATATGTAAATCATGAT+CGG + chr3_3:71827207-71827226 None:intergenic 20.0%
!!! TCATTTTTCTAAGAATTTGT+GGG - chr3_3:71827416-71827435 Msa0469830:intron 20.0%
!! TTTATTCTAGTATCATCAAA+TGG - chr3_3:71827531-71827550 Msa0469830:intron 20.0%
!! GAAAATTTACATACATATTG+TGG - chr3_3:71827670-71827689 Msa0469830:CDS 20.0%
! TTCTCTTCAATCACAAAAAA+TGG - chr3_3:71826246-71826265 Msa0469830:three_prime_UTR 25.0%
! ACTTACATGATTAATTTCCA+AGG + chr3_3:71826462-71826481 None:intergenic 25.0%
! GAAAACTACTGTTTGTAAAT+AGG - chr3_3:71826490-71826509 Msa0469830:CDS 25.0%
! ATAATCATTTAATCCACTCA+AGG + chr3_3:71826910-71826929 None:intergenic 25.0%
! GGATTAAATGATTATTTGTC+CGG - chr3_3:71826915-71826934 Msa0469830:intron 25.0%
! TTAAATGTATGATAAGGCTA+TGG + chr3_3:71827055-71827074 None:intergenic 25.0%
! GAAATACTAGAAAATCCATT+TGG + chr3_3:71827122-71827141 None:intergenic 25.0%
! TCATCGATAGATTCAAAATA+TGG - chr3_3:71827309-71827328 Msa0469830:intron 25.0%
! TATGGAAAATCAATAAAACG+AGG - chr3_3:71827327-71827346 Msa0469830:intron 25.0%
! CACAAATTCTTAGAAAAATG+AGG + chr3_3:71827417-71827436 None:intergenic 25.0%
!!! CTCATTTTTCTAAGAATTTG+TGG - chr3_3:71827415-71827434 Msa0469830:intron 25.0%
!!! CATTTTTCTAAGAATTTGTG+GGG - chr3_3:71827417-71827436 Msa0469830:intron 25.0%
! GATGATACTAGAATAAATGA+TGG + chr3_3:71827528-71827547 None:intergenic 25.0%
! TTCTCTTCAATCACAAAAAA+TGG - chr3_3:71826246-71826265 Msa0469830:three_prime_UTR 25.0%
! ACTTACATGATTAATTTCCA+AGG + chr3_3:71826462-71826481 None:intergenic 25.0%
! GAAAACTACTGTTTGTAAAT+AGG - chr3_3:71826490-71826509 Msa0469830:CDS 25.0%
! ATAATCATTTAATCCACTCA+AGG + chr3_3:71826910-71826929 None:intergenic 25.0%
! GGATTAAATGATTATTTGTC+CGG - chr3_3:71826915-71826934 Msa0469830:intron 25.0%
! TTAAATGTATGATAAGGCTA+TGG + chr3_3:71827055-71827074 None:intergenic 25.0%
! GAAATACTAGAAAATCCATT+TGG + chr3_3:71827122-71827141 None:intergenic 25.0%
! TCATCGATAGATTCAAAATA+TGG - chr3_3:71827309-71827328 Msa0469830:intron 25.0%
! TATGGAAAATCAATAAAACG+AGG - chr3_3:71827327-71827346 Msa0469830:intron 25.0%
! CACAAATTCTTAGAAAAATG+AGG + chr3_3:71827417-71827436 None:intergenic 25.0%
!!! CTCATTTTTCTAAGAATTTG+TGG - chr3_3:71827415-71827434 Msa0469830:intron 25.0%
!!! CATTTTTCTAAGAATTTGTG+GGG - chr3_3:71827417-71827436 Msa0469830:intron 25.0%
! GATGATACTAGAATAAATGA+TGG + chr3_3:71827528-71827547 None:intergenic 25.0%
TTATTACTATAGAGAAGGGT+GGG + chr3_3:71826190-71826209 None:intergenic 30.0%
ATTATTACTATAGAGAAGGG+TGG + chr3_3:71826191-71826210 None:intergenic 30.0%
! AACTTTTGATCTAACTGACT+TGG - chr3_3:71826513-71826532 Msa0469830:CDS 30.0%
!! TCTTATTTCTATCTCTCTCA+AGG - chr3_3:71826798-71826817 Msa0469830:intron 30.0%
TCTAAATCATCATGAACTAC+CGG + chr3_3:71826937-71826956 None:intergenic 30.0%
CTGGAGAATGATATATCTAA+GGG - chr3_3:71826966-71826985 Msa0469830:intron 30.0%
CATTGTCACAAACATTGAAA+AGG - chr3_3:71827027-71827046 Msa0469830:intron 30.0%
! TCTCATTTTCTGTTTCCAAA+TGG - chr3_3:71827104-71827123 Msa0469830:intron 30.0%
AATATGTAAATCATGATCGG+AGG + chr3_3:71827204-71827223 None:intergenic 30.0%
AATCTATCGATGATGTGTTT+CGG + chr3_3:71827302-71827321 None:intergenic 30.0%
CATTGTAAGCATAACCAATA+TGG + chr3_3:71827488-71827507 None:intergenic 30.0%
! CATATTGGTTATGCTTACAA+TGG - chr3_3:71827486-71827505 Msa0469830:intron 30.0%
ATGCACTATCACACAAATAT+AGG + chr3_3:71827716-71827735 None:intergenic 30.0%
TTATTACTATAGAGAAGGGT+GGG + chr3_3:71826190-71826209 None:intergenic 30.0%
ATTATTACTATAGAGAAGGG+TGG + chr3_3:71826191-71826210 None:intergenic 30.0%
! AACTTTTGATCTAACTGACT+TGG - chr3_3:71826513-71826532 Msa0469830:CDS 30.0%
!! TCTTATTTCTATCTCTCTCA+AGG - chr3_3:71826798-71826817 Msa0469830:intron 30.0%
TCTAAATCATCATGAACTAC+CGG + chr3_3:71826937-71826956 None:intergenic 30.0%
CTGGAGAATGATATATCTAA+GGG - chr3_3:71826966-71826985 Msa0469830:intron 30.0%
CATTGTCACAAACATTGAAA+AGG - chr3_3:71827027-71827046 Msa0469830:intron 30.0%
! TCTCATTTTCTGTTTCCAAA+TGG - chr3_3:71827104-71827123 Msa0469830:intron 30.0%
AATATGTAAATCATGATCGG+AGG + chr3_3:71827204-71827223 None:intergenic 30.0%
AATCTATCGATGATGTGTTT+CGG + chr3_3:71827302-71827321 None:intergenic 30.0%
CATTGTAAGCATAACCAATA+TGG + chr3_3:71827488-71827507 None:intergenic 30.0%
! CATATTGGTTATGCTTACAA+TGG - chr3_3:71827486-71827505 Msa0469830:intron 30.0%
ATGCACTATCACACAAATAT+AGG + chr3_3:71827716-71827735 None:intergenic 30.0%
TTGCAATGAGTAGTACTAGT+TGG - chr3_3:71826630-71826649 Msa0469830:CDS 35.0%
!! AGAGAAAATCAAGTGTTGGA+TGG + chr3_3:71826726-71826745 None:intergenic 35.0%
GAAGAGAGAAAATCAAGTGT+TGG + chr3_3:71826730-71826749 None:intergenic 35.0%
!! TGATAAGTTGATGATGTGAC+AGG + chr3_3:71826752-71826771 None:intergenic 35.0%
ATACACCTATTGGAATGCAT+AGG - chr3_3:71826824-71826843 Msa0469830:intron 35.0%
TGTTTCCTATGCATTCCAAT+AGG + chr3_3:71826832-71826851 None:intergenic 35.0%
CCTTAGATATATCATTCTCC+AGG + chr3_3:71826968-71826987 None:intergenic 35.0%
CCTGGAGAATGATATATCTA+AGG - chr3_3:71826965-71826984 Msa0469830:intron 35.0%
AAATCATGATCGGAGGAATT+AGG + chr3_3:71827197-71827216 None:intergenic 35.0%
!!! TACTGTGTTTTTTACAGCTG+CGG - chr3_3:71827240-71827259 Msa0469830:intron 35.0%
ATGTTGTATTTCACACGAGA+CGG + chr3_3:71827585-71827604 None:intergenic 35.0%
TATCCCATTTCCACATTCTT+GGG + chr3_3:71827637-71827656 None:intergenic 35.0%
ATATCCCATTTCCACATTCT+TGG + chr3_3:71827638-71827657 None:intergenic 35.0%
TAATACTCACCAGCAACAAA+AGG - chr3_3:71827741-71827760 Msa0469830:CDS 35.0%
! AAGAATACACCTTTTGTTGC+TGG + chr3_3:71827753-71827772 None:intergenic 35.0%
TACCAAAGCAACAAAGATGT+TGG - chr3_3:71827774-71827793 Msa0469830:five_prime_UTR 35.0%
ACCAAAGCAACAAAGATGTT+GGG - chr3_3:71827775-71827794 Msa0469830:five_prime_UTR 35.0%
TTGCAATGAGTAGTACTAGT+TGG - chr3_3:71826630-71826649 Msa0469830:CDS 35.0%
!! AGAGAAAATCAAGTGTTGGA+TGG + chr3_3:71826726-71826745 None:intergenic 35.0%
GAAGAGAGAAAATCAAGTGT+TGG + chr3_3:71826730-71826749 None:intergenic 35.0%
!! TGATAAGTTGATGATGTGAC+AGG + chr3_3:71826752-71826771 None:intergenic 35.0%
ATACACCTATTGGAATGCAT+AGG - chr3_3:71826824-71826843 Msa0469830:intron 35.0%
TGTTTCCTATGCATTCCAAT+AGG + chr3_3:71826832-71826851 None:intergenic 35.0%
CCTTAGATATATCATTCTCC+AGG + chr3_3:71826968-71826987 None:intergenic 35.0%
CCTGGAGAATGATATATCTA+AGG - chr3_3:71826965-71826984 Msa0469830:intron 35.0%
AAATCATGATCGGAGGAATT+AGG + chr3_3:71827197-71827216 None:intergenic 35.0%
!!! TACTGTGTTTTTTACAGCTG+CGG - chr3_3:71827240-71827259 Msa0469830:intron 35.0%
ATGTTGTATTTCACACGAGA+CGG + chr3_3:71827585-71827604 None:intergenic 35.0%
TATCCCATTTCCACATTCTT+GGG + chr3_3:71827637-71827656 None:intergenic 35.0%
ATATCCCATTTCCACATTCT+TGG + chr3_3:71827638-71827657 None:intergenic 35.0%
TAATACTCACCAGCAACAAA+AGG - chr3_3:71827741-71827760 Msa0469830:CDS 35.0%
! AAGAATACACCTTTTGTTGC+TGG + chr3_3:71827753-71827772 None:intergenic 35.0%
TACCAAAGCAACAAAGATGT+TGG - chr3_3:71827774-71827793 Msa0469830:five_prime_UTR 35.0%
ACCAAAGCAACAAAGATGTT+GGG - chr3_3:71827775-71827794 Msa0469830:five_prime_UTR 35.0%
AAGAAGAGGAGGATGACAAA+GGG + chr3_3:71826312-71826331 None:intergenic 40.0%
AGAAGTAGTAGAAGAAGAGG+AGG + chr3_3:71826323-71826342 None:intergenic 40.0%
CCTCTTCTTCTACTACTTCT+CGG - chr3_3:71826323-71826342 Msa0469830:three_prime_UTR 40.0%
CTCAAGGTGTATACACCTAT+TGG - chr3_3:71826814-71826833 Msa0469830:intron 40.0%
TGCTTAGGTTTCATTCTCAG+AGG - chr3_3:71826862-71826881 Msa0469830:intron 40.0%
GCTTAGGTTTCATTCTCAGA+GGG - chr3_3:71826863-71826882 Msa0469830:intron 40.0%
!! CAGTAAAAAAGCACCTTGAG+TGG - chr3_3:71826894-71826913 Msa0469830:intron 40.0%
ATGATTTAGATGCAAGAGCC+TGG - chr3_3:71826947-71826966 Msa0469830:intron 40.0%
GGTTTGATGAGGATAGGTTA+AGG + chr3_3:71827281-71827300 None:intergenic 40.0%
! TGTTTCGGTTTGATGAGGAT+AGG + chr3_3:71827287-71827306 None:intergenic 40.0%
! TGATGTGTTTCGGTTTGATG+AGG + chr3_3:71827292-71827311 None:intergenic 40.0%
! TCGTCAACATTGATGATAGC+AGG - chr3_3:71827361-71827380 Msa0469830:intron 40.0%
TCTTCCCAAGAATGTGGAAA+TGG - chr3_3:71827630-71827649 Msa0469830:intron 40.0%
CTTCCCAAGAATGTGGAAAT+GGG - chr3_3:71827631-71827650 Msa0469830:intron 40.0%
TGGAAATGGGATATCTGTCT+TGG - chr3_3:71827644-71827663 Msa0469830:intron 40.0%
! TCCCAACATCTTTGTTGCTT+TGG + chr3_3:71827779-71827798 None:intergenic 40.0%
AAGAAGAGGAGGATGACAAA+GGG + chr3_3:71826312-71826331 None:intergenic 40.0%
AGAAGTAGTAGAAGAAGAGG+AGG + chr3_3:71826323-71826342 None:intergenic 40.0%
CCTCTTCTTCTACTACTTCT+CGG - chr3_3:71826323-71826342 Msa0469830:three_prime_UTR 40.0%
CTCAAGGTGTATACACCTAT+TGG - chr3_3:71826814-71826833 Msa0469830:intron 40.0%
TGCTTAGGTTTCATTCTCAG+AGG - chr3_3:71826862-71826881 Msa0469830:intron 40.0%
GCTTAGGTTTCATTCTCAGA+GGG - chr3_3:71826863-71826882 Msa0469830:intron 40.0%
!! CAGTAAAAAAGCACCTTGAG+TGG - chr3_3:71826894-71826913 Msa0469830:intron 40.0%
ATGATTTAGATGCAAGAGCC+TGG - chr3_3:71826947-71826966 Msa0469830:intron 40.0%
GGTTTGATGAGGATAGGTTA+AGG + chr3_3:71827281-71827300 None:intergenic 40.0%
! TGTTTCGGTTTGATGAGGAT+AGG + chr3_3:71827287-71827306 None:intergenic 40.0%
! TGATGTGTTTCGGTTTGATG+AGG + chr3_3:71827292-71827311 None:intergenic 40.0%
! TCGTCAACATTGATGATAGC+AGG - chr3_3:71827361-71827380 Msa0469830:intron 40.0%
TCTTCCCAAGAATGTGGAAA+TGG - chr3_3:71827630-71827649 Msa0469830:intron 40.0%
CTTCCCAAGAATGTGGAAAT+GGG - chr3_3:71827631-71827650 Msa0469830:intron 40.0%
TGGAAATGGGATATCTGTCT+TGG - chr3_3:71827644-71827663 Msa0469830:intron 40.0%
! TCCCAACATCTTTGTTGCTT+TGG + chr3_3:71827779-71827798 None:intergenic 40.0%
TTACTATAGAGAAGGGTGGG+AGG + chr3_3:71826187-71826206 None:intergenic 45.0%
GAAGAAGAGGAGGATGACAA+AGG + chr3_3:71826313-71826332 None:intergenic 45.0%
CCGAGAAGTAGTAGAAGAAG+AGG + chr3_3:71826326-71826345 None:intergenic 45.0%
CTTCTACTACTTCTCGGACT+CGG - chr3_3:71826329-71826348 Msa0469830:three_prime_UTR 45.0%
TTTGCAACCAACGAAGAGAG+TGG + chr3_3:71826383-71826402 None:intergenic 45.0%
TGATGATAGCAGGTTTGCAC+AGG - chr3_3:71827371-71827390 Msa0469830:intron 45.0%
! CGCAAGTGTTGCAACCATAT+TGG - chr3_3:71827471-71827490 Msa0469830:intron 45.0%
CCACATTCTTGGGAAGATGA+TGG + chr3_3:71827627-71827646 None:intergenic 45.0%
CCATCATCTTCCCAAGAATG+TGG - chr3_3:71827624-71827643 Msa0469830:intron 45.0%
TTACTATAGAGAAGGGTGGG+AGG + chr3_3:71826187-71826206 None:intergenic 45.0%
GAAGAAGAGGAGGATGACAA+AGG + chr3_3:71826313-71826332 None:intergenic 45.0%
CCGAGAAGTAGTAGAAGAAG+AGG + chr3_3:71826326-71826345 None:intergenic 45.0%
CTTCTACTACTTCTCGGACT+CGG - chr3_3:71826329-71826348 Msa0469830:three_prime_UTR 45.0%
TTTGCAACCAACGAAGAGAG+TGG + chr3_3:71826383-71826402 None:intergenic 45.0%
TGATGATAGCAGGTTTGCAC+AGG - chr3_3:71827371-71827390 Msa0469830:intron 45.0%
! CGCAAGTGTTGCAACCATAT+TGG - chr3_3:71827471-71827490 Msa0469830:intron 45.0%
CCACATTCTTGGGAAGATGA+TGG + chr3_3:71827627-71827646 None:intergenic 45.0%
CCATCATCTTCCCAAGAATG+TGG - chr3_3:71827624-71827643 Msa0469830:intron 45.0%
TTCTCGGACTCGGAGTCAAA+GGG - chr3_3:71826339-71826358 Msa0469830:exon 50.0%
AGAGAGTGGTGAACCTGTAG+CGG + chr3_3:71826369-71826388 None:intergenic 50.0%
AGGTTCACCACTCTCTTCGT+TGG - chr3_3:71826373-71826392 Msa0469830:CDS 50.0%
! TTTTACAGCTGCGGTTCGTG+CGG - chr3_3:71827249-71827268 Msa0469830:intron 50.0%
GGTGCTTCAGTTGAAGGAGA+TGG + chr3_3:71827564-71827583 None:intergenic 50.0%
! TTGGGAAGATGATGGCTGCA+AGG + chr3_3:71827619-71827638 None:intergenic 50.0%
TTCTCGGACTCGGAGTCAAA+GGG - chr3_3:71826339-71826358 Msa0469830:exon 50.0%
AGAGAGTGGTGAACCTGTAG+CGG + chr3_3:71826369-71826388 None:intergenic 50.0%
AGGTTCACCACTCTCTTCGT+TGG - chr3_3:71826373-71826392 Msa0469830:CDS 50.0%
! TTTTACAGCTGCGGTTCGTG+CGG - chr3_3:71827249-71827268 Msa0469830:intron 50.0%
GGTGCTTCAGTTGAAGGAGA+TGG + chr3_3:71827564-71827583 None:intergenic 50.0%
! TTGGGAAGATGATGGCTGCA+AGG + chr3_3:71827619-71827638 None:intergenic 50.0%
CTTCTCGGACTCGGAGTCAA+AGG - chr3_3:71826338-71826357 Msa0469830:exon 55.0%
GTCAAAGGGATGTCCGCTAC+AGG - chr3_3:71826353-71826372 Msa0469830:exon 55.0%
CGAGACGGTGCTTCAGTTGA+AGG + chr3_3:71827570-71827589 None:intergenic 55.0%
GAGAAGGGTGGGAGGAAAGA+AGG + chr3_3:71826179-71826198 None:intergenic 55.0%
CTTCTCGGACTCGGAGTCAA+AGG - chr3_3:71826338-71826357 Msa0469830:exon 55.0%
GTCAAAGGGATGTCCGCTAC+AGG - chr3_3:71826353-71826372 Msa0469830:exon 55.0%
CGAGACGGTGCTTCAGTTGA+AGG + chr3_3:71827570-71827589 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 71826177 71827851 71826177 ID=Msa0469830;Name=Msa0469830
chr3_3 mRNA 71826177 71827851 71826177 ID=Msa0469830-mRNA-1;Parent=Msa0469830;Name=Msa0469830-mRNA-1;_AED=0.02;_eAED=0.02;_QI=88|1|1|1|1|1|2|180|174
chr3_3 exon 71826177 71826771 71826177 ID=Msa0469830-mRNA-1:exon:17702;Parent=Msa0469830-mRNA-1
chr3_3 exon 71827654 71827851 71827654 ID=Msa0469830-mRNA-1:exon:17701;Parent=Msa0469830-mRNA-1
chr3_3 five_prime_UTR 71827764 71827851 71827764 ID=Msa0469830-mRNA-1:five_prime_utr;Parent=Msa0469830-mRNA-1
chr3_3 CDS 71827654 71827763 71827654 ID=Msa0469830-mRNA-1:cds;Parent=Msa0469830-mRNA-1
chr3_3 CDS 71826357 71826771 71826357 ID=Msa0469830-mRNA-1:cds;Parent=Msa0469830-mRNA-1
chr3_3 three_prime_UTR 71826177 71826356 71826177 ID=Msa0469830-mRNA-1:three_prime_utr;Parent=Msa0469830-mRNA-1
Gene Sequence

>Msa0469830

ATGGACAATTCAGTGTTTCACAATCACAACGTTGATCATCTTCACCCTTTGTCATCCTCCTCTTCTTCTACTACTTCTCGGACTCGGAGTCAAAGGGATGTCCGCTACAGCTGCGGTTCGTGCGGATATGACCTTAACCTATCCTCATCAAACCGAAACACATCATCGATAGATTCAAAATATGGAAAATCAATAAAACGAGGTATCATATCATTCGTCAACATTGATGATAGCAGGTTTGCACAGGTTGATGAATTTGAATGTGCTCCTCATTTTTCTAAGAATTTGTGGGGTTTGTTTCGACGAAAAACAAAGCTTCTTTGTCGCAAGTGTTGCAACCATATTGGTTATGCTTACAATGGTTACAATTCATCATCACCATCATTTATTCTAGTATCATCAAATGGAGCAGAACCATCTCCTTCAACTGAAGCACCGTCTCGTGTGAAATACAACATTCGCATTCGTGCCTTGCAGCCATCATCTTCCCAAGAATGTGGAAATGGGATATCTGTCTTGGCTTGA
Protein sequence

>Msa0469830

MDNSVFHNHNVDHLHPLSSSSSSTTSRTRSQRDVRYSCGSCGYDLNLSSSNRNTSSIDSKYGKSIKRGIISFVNIDDSRFAQVDEFECAPHFSKNLWGLFRRKTKLLCRKCCNHIGYAYNGYNSSSPSFILVSSNGAEPSPSTEAPSRVKYNIRIRALQPSSSQECGNGISVLA*