AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0475250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0475250 MtrunA17_Chr3g0132411 88.189 127 14 1 3 129 44 169 1.43e-76 224
Msa0475250 MtrunA17_Chr3g0132431 68.852 122 37 1 4 125 6 126 2.60e-52 163
Msa0475250 MtrunA17_Chr3g0132441 68.468 111 35 0 10 120 11 121 3.51e-49 155
Msa0475250 MtrunA17_Chr3g0132701 66.387 119 37 3 4 120 6 123 6.86e-44 143
Msa0475250 MtrunA17_Chr3g0132511 66.387 119 37 3 4 120 6 123 1.07e-43 142
Msa0475250 MtrunA17_Chr8g0335571 48.361 122 58 3 1 119 1 120 1.66e-30 108
Msa0475250 MtrunA17_Chr8g0335531 46.721 122 60 3 1 119 1 120 1.81e-27 100
Msa0475250 MtrunA17_Chr2g0323021 38.583 127 74 3 5 130 6 129 3.07e-23 90.5
Msa0475250 MtrunA17_Chr1g0196801 38.393 112 69 0 22 133 20 131 2.18e-22 87.0
Msa0475250 MtrunA17_Chr6g0459441 39.048 105 63 1 21 124 22 126 1.13e-20 82.8
Msa0475250 MtrunA17_Chr6g0481491 37.500 104 65 0 25 128 28 131 6.85e-20 81.6
Msa0475250 MtrunA17_Chr7g0253701 38.261 115 66 3 10 119 8 122 3.91e-18 75.9
Msa0475250 MtrunA17_Chr7g0253671 38.261 115 66 3 10 119 8 122 3.91e-18 75.9
Msa0475250 MtrunA17_Chr8g0387804 34.677 124 72 3 1 120 10 128 5.69e-18 74.3
Msa0475250 MtrunA17_Chr7g0253631 37.398 123 73 3 1 120 1 122 8.46e-18 76.6
Msa0475250 MtrunA17_Chr7g0253681 37.931 116 66 3 10 119 8 123 1.28e-17 74.3
Msa0475250 MtrunA17_Chr1g0177341 35.088 114 69 3 11 119 8 121 1.56e-17 73.9
Msa0475250 MtrunA17_Chr1g0177331 35.088 114 69 3 11 119 8 121 1.56e-17 73.9
Msa0475250 MtrunA17_Chr4g0064041 39.000 100 56 2 25 119 191 290 1.79e-17 77.0
Msa0475250 MtrunA17_Chr4g0064041 35.938 128 74 4 6 125 7 134 1.78e-16 74.3
Msa0475250 MtrunA17_Chr6g0453921 34.074 135 81 5 5 135 17 147 2.77e-17 73.9
Msa0475250 MtrunA17_Chr5g0394321 34.513 113 71 2 22 131 23 135 3.45e-17 73.9
Msa0475250 MtrunA17_Chr1g0205701 32.203 118 74 3 5 119 8 122 3.62e-17 73.9
Msa0475250 MtrunA17_Chr7g0253761 35.200 125 73 5 1 120 1 122 2.73e-16 71.2
Msa0475250 MtrunA17_Chr4g0041811 34.426 122 74 3 3 119 11 131 3.81e-16 72.0
Msa0475250 MtrunA17_Chr7g0253731 35.398 113 68 3 12 119 9 121 4.51e-16 70.5
Msa0475250 MtrunA17_Chr7g0253641 36.066 122 74 3 1 119 1 121 7.03e-16 71.2
Msa0475250 MtrunA17_Chr6g0453801 32.800 125 79 4 1 121 10 133 3.27e-15 68.6
Msa0475250 MtrunA17_Chr1g0211101 32.283 127 79 3 1 125 14 135 3.60e-15 68.6
Msa0475250 MtrunA17_Chr4g0001571 33.981 103 66 2 19 120 23 124 5.89e-14 65.1
Msa0475250 MtrunA17_Chr6g0458331 33.981 103 62 2 22 119 34 135 8.49e-14 64.7
Msa0475250 MtrunA17_Chr2g0289741 29.688 128 80 3 1 126 11 130 1.34e-13 64.3
Msa0475250 MtrunA17_Chr8g0380901 38.136 118 66 5 2 118 8 119 2.50e-13 62.4
Msa0475250 MtrunA17_Chr2g0301431 36.134 119 69 4 3 120 13 125 3.38e-13 62.0
Msa0475250 MtrunA17_Chr4g0073921 33.058 121 74 4 6 119 9 129 6.65e-13 63.9
Msa0475250 MtrunA17_Chr8g0360371 31.405 121 79 3 3 119 1 121 7.55e-13 63.9
Msa0475250 MtrunA17_Chr6g0483981 35.246 122 71 5 7 120 8 129 2.10e-12 63.2
Msa0475250 MtrunA17_Chr4g0073901 33.019 106 65 3 22 121 26 131 2.39e-12 61.2
Msa0475250 MtrunA17_Chr2g0331181 35.000 100 59 2 25 119 27 125 2.60e-12 61.2
Msa0475250 MtrunA17_Chr2g0319611 34.314 102 60 2 25 119 26 127 4.52e-12 61.2
Msa0475250 MtrunA17_Chr2g0306461 34.951 103 64 2 20 119 23 125 4.63e-12 60.5
Msa0475250 MtrunA17_Chr2g0324711 35.714 98 60 2 25 119 25 122 7.59e-12 61.2
Msa0475250 MtrunA17_Chr4g0039721 25.547 137 94 3 5 134 12 147 2.63e-11 58.2
Msa0475250 MtrunA17_Chr1g0188981 29.703 101 65 2 25 119 29 129 3.72e-11 58.2
Msa0475250 MtrunA17_Chr3g0125761 33.051 118 74 4 1 117 10 123 8.68e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0475250 AT1G72230.1 51.429 105 51 0 33 137 31 135 1.54e-30 108
Msa0475250 AT1G22480.1 53.125 96 45 0 30 125 24 119 4.60e-26 97.1
Msa0475250 AT3G60270.1 47.475 99 52 0 21 119 22 120 2.59e-24 92.8
Msa0475250 AT3G27200.1 35.537 121 76 2 1 119 1 121 4.35e-22 86.7
Msa0475250 AT5G07475.1 39.286 112 68 0 22 133 27 138 2.16e-21 85.5
Msa0475250 AT2G44790.1 47.872 94 47 1 33 124 36 129 6.46e-20 81.6
Msa0475250 AT2G32300.1 36.441 118 73 2 20 136 21 137 2.25e-19 81.6
Msa0475250 AT2G32300.2 36.441 118 73 2 20 136 51 167 3.37e-19 81.6
Msa0475250 AT3G60280.1 40.404 99 59 0 21 119 20 118 1.35e-18 78.6
Msa0475250 AT2G31050.1 40.777 103 59 2 23 123 27 129 1.30e-17 75.5
Msa0475250 AT5G26330.1 38.614 101 59 2 22 119 21 121 5.42e-17 73.9
Msa0475250 AT1G64640.1 37.383 107 63 2 22 124 30 136 6.06e-16 71.2
Msa0475250 AT3G17675.1 32.759 116 77 1 5 119 12 127 7.66e-16 69.3
Msa0475250 AT5G53870.2 33.333 129 71 3 2 119 3 127 2.61e-15 71.6
Msa0475250 AT4G12880.1 34.711 121 71 5 4 120 10 126 2.91e-15 68.2
Msa0475250 AT5G53870.1 33.333 129 71 3 2 119 3 127 3.65e-15 71.2
Msa0475250 AT5G15350.1 33.884 121 72 5 4 120 10 126 4.77e-15 68.2
Msa0475250 AT2G26720.1 34.615 104 66 2 22 123 26 129 8.58e-15 68.6
Msa0475250 AT4G28365.1 34.694 98 61 2 25 119 29 126 3.89e-14 66.6
Msa0475250 AT3G01070.1 36.441 118 68 3 6 120 12 125 4.22e-14 65.9
Msa0475250 AT1G45063.2 35.897 117 69 4 25 137 149 263 1.56e-13 65.9
Msa0475250 AT1G45063.2 36.893 103 58 3 22 119 26 126 1.72e-12 63.2
Msa0475250 AT1G45063.1 35.897 117 69 4 25 137 149 263 1.80e-13 66.2
Msa0475250 AT1G45063.1 36.893 103 58 3 22 119 26 126 1.47e-12 63.9
Msa0475250 AT2G25060.1 32.727 110 69 2 28 132 35 144 3.31e-13 63.5
Msa0475250 AT4G32490.1 31.356 118 77 3 5 119 12 128 3.46e-13 64.3
Msa0475250 AT3G20570.1 32.203 118 72 5 6 119 15 128 5.31e-13 63.5
Msa0475250 AT1G79800.1 31.148 122 78 2 3 119 12 132 6.42e-13 63.2
Msa0475250 AT4G31840.1 32.558 129 74 4 1 121 6 129 1.12e-12 62.4
Msa0475250 AT1G17800.1 35.833 120 74 3 1 118 17 135 5.79e-12 59.7
Msa0475250 AT4G30590.1 32.000 100 62 3 26 119 29 128 5.84e-12 60.5
Msa0475250 AT4G27520.1 35.200 125 73 3 3 119 4 128 7.87e-12 61.6
Msa0475250 AT3G18590.1 28.689 122 80 2 4 119 6 126 1.97e-11 58.9
Msa0475250 AT5G57920.1 38.272 81 49 1 40 119 43 123 2.11e-11 58.9
Msa0475250 AT5G57920.2 38.272 81 49 1 40 119 42 122 2.78e-11 58.5
Msa0475250 AT5G57920.3 38.272 81 49 1 40 119 75 155 2.94e-11 58.9
Msa0475250 AT5G20230.1 39.000 100 55 4 25 119 25 123 3.27e-11 58.5

Find 39 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTACTGTCTGAACTAAT+TGG 0.240452 3_3:+78379052 None:intergenic
AATATGACAGTGTTTCTCTA+TGG 0.243627 3_3:-78379136 Msa0475250:intron
GACTACAGCTCCTGCACTAC+TGG 0.377914 3_3:-78379079 Msa0475250:CDS
GATTCCTTAGGCTGGCAAAT+TGG 0.388696 3_3:-78379373 Msa0475250:CDS
TGGCATGCGTCTTGCTGTTA+AGG 0.410850 3_3:-78378957 Msa0475250:CDS
TACATTGTGGGAGATTCCTT+AGG 0.413259 3_3:-78379385 Msa0475250:CDS
TGGCAAATTGGTGTTGAATA+TGG 0.413393 3_3:-78379361 Msa0475250:CDS
TACTTCATATCTGCTACTCT+TGG 0.416128 3_3:-78378992 Msa0475250:CDS
GGAATCTCCCACAATGTAGA+AGG 0.425420 3_3:+78379390 None:intergenic
ATATGACAGTGTTTCTCTAT+GGG 0.434671 3_3:-78379135 Msa0475250:intron
TTGAATATGGCACATGGACC+AGG 0.462025 3_3:-78379348 Msa0475250:CDS
TCATCAAATGGCATTGGCTA+TGG 0.463785 3_3:-78379464 None:intergenic
CTTGCAACCTTCTACATTGT+GGG 0.481023 3_3:-78379397 Msa0475250:CDS
AACACCAATTTGCCAGCCTA+AGG 0.495237 3_3:+78379369 None:intergenic
CATCGTCATCAGCGAAGGAA+AGG 0.510283 3_3:-78378907 Msa0475250:CDS
AATTGGATTTCCAGTAGTGC+AGG 0.512919 3_3:+78379069 None:intergenic
ATCATTGTTCTCAAGACTGC+AGG 0.518138 3_3:-78379022 Msa0475250:CDS
TTGTGGGAGATTCCTTAGGC+TGG 0.542596 3_3:-78379381 Msa0475250:CDS
CTTGCTGTTAAGGTTGAGAC+TGG 0.543826 3_3:-78378947 Msa0475250:CDS
CCTTGCAACCTTCTACATTG+TGG 0.550975 3_3:-78379398 Msa0475250:CDS
ACTATCTCATCAAATGGCAT+TGG 0.552933 3_3:-78379470 None:intergenic
ATCGTCATCAGCGAAGGAAA+GGG 0.562412 3_3:-78378906 Msa0475250:CDS
ACAGCAAGACGCATGCCACT+TGG 0.564795 3_3:+78378962 None:intergenic
ATGTAGAAGGTTGCAAGGGT+TGG 0.572218 3_3:+78379403 None:intergenic
CCACAATGTAGAAGGTTGCA+AGG 0.572261 3_3:+78379398 None:intergenic
GTGGAGGGAGTGCCATCAGA+AGG 0.581640 3_3:+78378880 None:intergenic
TGCAACATCGTCATCAGCGA+AGG 0.584538 3_3:-78378912 Msa0475250:CDS
AGGAAAGGGTTACCTTCTGA+TGG 0.599662 3_3:-78378892 None:intergenic
TTTGTAGTCGGTGATAGCCT+CGG 0.600744 3_3:-78379316 Msa0475250:intron
CACAATGTAGAAGGTTGCAA+GGG 0.617890 3_3:+78379399 None:intergenic
ACTTGCTGCAACTTCATCCA+CGG 0.629770 3_3:+78379102 None:intergenic
TATGACAGTGTTTCTCTATG+GGG 0.630632 3_3:-78379134 Msa0475250:intron
AGGGACAAAACCTTTGTAGT+CGG 0.651766 3_3:-78379328 Msa0475250:CDS
CCAATTAGTTCAGACAGTAG+TGG 0.652205 3_3:-78379052 Msa0475250:CDS
ACTCTTGGAGATTGTCCAAG+TGG 0.659300 3_3:-78378977 Msa0475250:CDS
GAGGCTATCACCGACTACAA+AGG 0.672353 3_3:+78379318 None:intergenic
TGAATATGGCACATGGACCA+GGG 0.694459 3_3:-78379347 Msa0475250:CDS
TTGGTGTTGAATATGGCACA+TGG 0.722849 3_3:-78379354 Msa0475250:CDS
CTATGGGGCTAGACACACCG+TGG 0.749227 3_3:-78379119 Msa0475250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTCTTTTTAGCAATCAACA+TGG - chr3_3:78378921-78378940 Msa0475250:CDS 30.0%
AATATGACAGTGTTTCTCTA+TGG - chr3_3:78379216-78379235 Msa0475250:intron 30.0%
ATATGACAGTGTTTCTCTAT+GGG - chr3_3:78379217-78379236 Msa0475250:intron 30.0%
!! TGGCAAATTGGTGTTGAATA+TGG - chr3_3:78378991-78379010 Msa0475250:CDS 35.0%
! GATATGCTTTGTTCTCGTTA+TGG + chr3_3:78379126-78379145 None:intergenic 35.0%
TATGACAGTGTTTCTCTATG+GGG - chr3_3:78379218-78379237 Msa0475250:intron 35.0%
TACTTCATATCTGCTACTCT+TGG - chr3_3:78379360-78379379 Msa0475250:CDS 35.0%
CACAATGTAGAAGGTTGCAA+GGG + chr3_3:78378956-78378975 None:intergenic 40.0%
CTTGCAACCTTCTACATTGT+GGG - chr3_3:78378955-78378974 Msa0475250:CDS 40.0%
TACATTGTGGGAGATTCCTT+AGG - chr3_3:78378967-78378986 Msa0475250:CDS 40.0%
!! TTGGTGTTGAATATGGCACA+TGG - chr3_3:78378998-78379017 Msa0475250:CDS 40.0%
AGGGACAAAACCTTTGTAGT+CGG - chr3_3:78379024-78379043 Msa0475250:CDS 40.0%
AATTGGATTTCCAGTAGTGC+AGG + chr3_3:78379286-78379305 None:intergenic 40.0%
CCACTACTGTCTGAACTAAT+TGG + chr3_3:78379303-78379322 None:intergenic 40.0%
CCAATTAGTTCAGACAGTAG+TGG - chr3_3:78379300-78379319 Msa0475250:intron 40.0%
ATCATTGTTCTCAAGACTGC+AGG - chr3_3:78379330-78379349 Msa0475250:CDS 40.0%
ATGTAGAAGGTTGCAAGGGT+TGG + chr3_3:78378952-78378971 None:intergenic 45.0%
CCACAATGTAGAAGGTTGCA+AGG + chr3_3:78378957-78378976 None:intergenic 45.0%
CCTTGCAACCTTCTACATTG+TGG - chr3_3:78378954-78378973 Msa0475250:CDS 45.0%
GGAATCTCCCACAATGTAGA+AGG + chr3_3:78378965-78378984 None:intergenic 45.0%
GATTCCTTAGGCTGGCAAAT+TGG - chr3_3:78378979-78378998 Msa0475250:CDS 45.0%
AACACCAATTTGCCAGCCTA+AGG + chr3_3:78378986-78379005 None:intergenic 45.0%
TTGAATATGGCACATGGACC+AGG - chr3_3:78379004-78379023 Msa0475250:CDS 45.0%
TGAATATGGCACATGGACCA+GGG - chr3_3:78379005-78379024 Msa0475250:CDS 45.0%
!! GACTACAAAGGTTTTGTCCC+TGG + chr3_3:78379025-78379044 None:intergenic 45.0%
TTTGTAGTCGGTGATAGCCT+CGG - chr3_3:78379036-78379055 Msa0475250:CDS 45.0%
TGATAGCCTCGGTAAGAACT+AGG - chr3_3:78379047-78379066 Msa0475250:CDS 45.0%
! TTAGCTCCTAGTTCTTACCG+AGG + chr3_3:78379056-78379075 None:intergenic 45.0%
! ACTTGCTGCAACTTCATCCA+CGG + chr3_3:78379253-78379272 None:intergenic 45.0%
ACTCTTGGAGATTGTCCAAG+TGG - chr3_3:78379375-78379394 Msa0475250:CDS 45.0%
!! CTTGCTGTTAAGGTTGAGAC+TGG - chr3_3:78379405-78379424 Msa0475250:CDS 45.0%
ATCGTCATCAGCGAAGGAAA+GGG - chr3_3:78379446-78379465 Msa0475250:CDS 45.0%
TTGTGGGAGATTCCTTAGGC+TGG - chr3_3:78378971-78378990 Msa0475250:CDS 50.0%
! GAGGCTATCACCGACTACAA+AGG + chr3_3:78379037-78379056 None:intergenic 50.0%
!! TGGCATGCGTCTTGCTGTTA+AGG - chr3_3:78379395-78379414 Msa0475250:CDS 50.0%
TGCAACATCGTCATCAGCGA+AGG - chr3_3:78379440-78379459 Msa0475250:CDS 50.0%
CATCGTCATCAGCGAAGGAA+AGG - chr3_3:78379445-78379464 Msa0475250:CDS 50.0%
GACTACAGCTCCTGCACTAC+TGG - chr3_3:78379273-78379292 Msa0475250:intron 55.0%
ACAGCAAGACGCATGCCACT+TGG + chr3_3:78379393-78379412 None:intergenic 55.0%
CTATGGGGCTAGACACACCG+TGG - chr3_3:78379233-78379252 Msa0475250:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 78378895 78379479 78378895 ID=Msa0475250;Name=Msa0475250
chr3_3 mRNA 78378895 78379479 78378895 ID=Msa0475250-mRNA-1;Parent=Msa0475250;Name=Msa0475250-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|2|0|138
chr3_3 exon 78379317 78379479 78379317 ID=Msa0475250-mRNA-1:exon:21129;Parent=Msa0475250-mRNA-1
chr3_3 exon 78378895 78379148 78378895 ID=Msa0475250-mRNA-1:exon:21128;Parent=Msa0475250-mRNA-1
chr3_3 CDS 78379317 78379479 78379317 ID=Msa0475250-mRNA-1:cds;Parent=Msa0475250-mRNA-1
chr3_3 CDS 78378895 78379148 78378895 ID=Msa0475250-mRNA-1:cds;Parent=Msa0475250-mRNA-1
Gene Sequence

>Msa0475250

ATGGCATTGGCTATGGTTTTGTGCTTCTTCTTTTTAGCAATCAACATGGCACTGCCAACCCTTGCAACCTTCTACATTGTGGGAGATTCCTTAGGCTGGCAAATTGGTGTTGAATATGGCACATGGACCAGGGACAAAACCTTTGTAGTCGGTGATAGCCTCGTGTTTCTCTATGGGGCTAGACACACCGTGGATGAAGTTGCAGCAAGTGACTACAGCTCCTGCACTACTGGAAATCCAATTAGTTCAGACAGTAGTGGTGAGACAATCATTGTTCTCAAGACTGCAGGAACTCATTACTTCATATCTGCTACTCTTGGAGATTGTCCAAGTGGCATGCGTCTTGCTGTTAAGGTTGAGACTGGTAGTGCTTCTATTGCAACATCGTCATCAGCGAAGGAAAGGGTTACCTTCTGA

Protein sequence

>Msa0475250

MALAMVLCFFFLAINMALPTLATFYIVGDSLGWQIGVEYGTWTRDKTFVVGDSLVFLYGARHTVDEVAASDYSSCTTGNPISSDSSGETIIVLKTAGTHYFISATLGDCPSGMRLAVKVETGSASIATSSSAKERVTF*