AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0477190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0477190 MtrunA17_Chr3g0139081 98.690 687 9 0 1 687 1 687 0.0 1394
Msa0477190 MtrunA17_Chr4g0000581 73.969 703 163 7 1 687 1 699 0.0 1037
Msa0477190 MtrunA17_Chr3g0135191 64.930 710 214 10 1 687 1 698 0.0 892
Msa0477190 MtrunA17_Chr6g0451381 66.568 673 208 6 19 687 7 666 0.0 890
Msa0477190 MtrunA17_Chr8g0339101 60.508 709 247 8 2 687 4 702 0.0 833
Msa0477190 MtrunA17_Chr1g0179381 42.293 532 270 5 155 683 19 516 2.25e-143 428
Msa0477190 MtrunA17_Chr1g0179441 41.917 532 273 5 155 683 19 517 1.17e-142 427
Msa0477190 MtrunA17_Chr1g0179421 41.353 532 276 4 155 683 19 517 8.46e-142 424
Msa0477190 MtrunA17_Chr1g0198111 45.188 478 229 4 207 683 79 524 1.96e-141 424
Msa0477190 MtrunA17_Chr7g0265711 41.729 532 274 5 155 683 28 526 1.31e-140 422
Msa0477190 MtrunA17_Chr4g0027221 40.968 537 284 7 155 683 28 539 2.82e-135 408
Msa0477190 MtrunA17_Chr7g0264621 39.147 539 281 5 154 683 24 524 1.65e-134 406
Msa0477190 MtrunA17_Chr1g0179391 49.211 317 160 1 267 582 1 317 9.12e-108 330
Msa0477190 MtrunA17_Chr4g0064791 72.535 142 33 1 189 324 59 200 5.86e-63 208
Msa0477190 MtrunA17_Chr4g0037831 79.808 104 21 0 42 145 7 110 2.15e-49 168
Msa0477190 MtrunA17_Chr4g0060331 76.667 90 21 0 42 131 7 96 1.60e-37 135
Msa0477190 MtrunA17_Chr1g0179401 41.379 116 65 2 155 268 19 133 6.73e-19 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0477190 AT4G31590.1 79.365 693 136 5 1 687 1 692 0.0 1097
Msa0477190 AT2G24630.1 76.590 692 155 5 1 687 1 690 0.0 1080
Msa0477190 AT2G24630.2 76.590 692 155 5 1 687 1 690 0.0 1080
Msa0477190 AT3G28180.1 66.183 689 190 8 21 687 6 673 0.0 906
Msa0477190 AT4G07960.1 64.034 709 221 9 1 686 1 698 0.0 873
Msa0477190 AT4G07960.2 64.034 709 221 9 1 686 1 698 0.0 873
Msa0477190 AT3G07330.1 61.696 684 251 5 6 687 8 682 0.0 853
Msa0477190 AT3G07330.2 61.696 684 251 5 6 687 8 682 0.0 853
Msa0477190 AT5G03760.1 42.830 530 263 9 159 683 33 527 1.93e-141 424
Msa0477190 AT5G22740.1 41.165 532 279 5 155 683 29 529 5.98e-139 418
Msa0477190 AT1G23480.1 41.078 538 279 6 150 683 47 550 2.29e-137 414
Msa0477190 AT1G23480.2 41.078 538 279 6 150 683 47 550 2.29e-137 414
Msa0477190 AT1G23480.3 43.841 479 234 4 207 683 33 478 3.39e-135 406
Msa0477190 AT2G35650.1 46.469 439 231 3 155 590 51 488 6.93e-134 405
Msa0477190 AT4G13410.1 43.290 462 248 3 155 608 43 498 2.86e-133 403
Msa0477190 AT2G35650.4 50.263 380 188 1 212 590 37 416 2.43e-132 399
Msa0477190 AT2G35650.2 50.263 380 188 1 212 590 37 416 2.43e-132 399
Msa0477190 AT5G16190.3 40.301 531 282 4 155 683 6 503 8.89e-132 398
Msa0477190 AT1G24070.1 38.752 529 287 5 158 683 57 551 1.13e-131 400
Msa0477190 AT5G16190.1 42.283 473 239 3 212 683 3 442 6.01e-129 389
Msa0477190 AT3G56000.1 40.262 534 274 7 159 683 26 523 6.74e-129 392
Msa0477190 AT5G16190.2 45.147 443 241 2 155 595 6 448 8.25e-129 389
Msa0477190 AT5G16190.4 37.943 564 282 5 155 683 6 536 7.28e-125 382
Msa0477190 AT4G16590.1 37.037 540 268 8 147 683 49 519 2.47e-112 348
Msa0477190 AT1G23480.4 42.105 418 207 4 268 683 21 405 8.23e-110 338
Msa0477190 AT2G35650.3 48.137 322 166 1 270 590 9 330 5.36e-105 325
Msa0477190 AT4G13410.3 45.536 336 182 1 274 608 2 337 9.87e-105 324
Msa0477190 AT1G24070.2 47.962 319 163 2 158 474 57 374 1.64e-100 314
Msa0477190 AT4G13410.2 47.401 327 159 2 155 474 43 363 4.36e-99 310

Find 187 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCAGTGTCACTAATTT+TGG 0.029150 3_3:-81358547 None:intergenic
TACTTTCAAGGTTGCCATTT+TGG 0.090610 3_3:-81357811 Msa0477190:CDS
AATCTTCACAACTGAGAAAT+TGG 0.125987 3_3:+81358101 None:intergenic
GTTAATTTCTTTGGCTGTTT+TGG 0.142194 3_3:-81357851 Msa0477190:CDS
ACCGTATGCTTCTTTGTTTC+GGG 0.170225 3_3:-81357633 Msa0477190:CDS
CAGAGGCAGAACTTCCTTTA+TGG 0.175113 3_3:-81355722 Msa0477190:CDS
TTCTCAGTTGTGAAGATTAA+TGG 0.191808 3_3:-81358096 Msa0477190:CDS
CAGCAACACCGCTGGCATTC+AGG 0.192229 3_3:-81356225 Msa0477190:CDS
CTTGGTTAGTAACTGTTCTT+TGG 0.224084 3_3:-81357969 Msa0477190:CDS
GGGGTGTTTCTTAATTTCTT+TGG 0.229657 3_3:-81356600 Msa0477190:CDS
TTAGCTTTCTTAACAGGTTC+TGG 0.235289 3_3:+81355378 None:intergenic
AAATAAGGTGAAGGAGTAAA+AGG 0.246621 3_3:+81355776 None:intergenic
TTCTTTGTTTCGGGTGGTTT+TGG 0.256673 3_3:-81357624 Msa0477190:CDS
GGAAGCATAAATTTATGTTT+TGG 0.261801 3_3:+81356649 None:intergenic
AAATTTAATGCAATGGTATC+TGG 0.270707 3_3:-81355588 Msa0477190:CDS
CCTGCTGTTCTACTTCAAAA+TGG 0.273610 3_3:+81356628 None:intergenic
AGATTCGTAGTTGATGTTAT+TGG 0.273969 3_3:+81358379 None:intergenic
CAGGGAGTGGCTGTTGAAAA+AGG 0.279089 3_3:-81357907 Msa0477190:CDS
ATGCATTTATCCAATGGTTT+TGG 0.280954 3_3:-81357530 Msa0477190:CDS
TTTGTTCTTTGGTTGTATTT+GGG 0.283510 3_3:-81354770 Msa0477190:three_prime_UTR
CTTAATTTCTTTGGTTTCAA+TGG 0.284700 3_3:-81356591 Msa0477190:CDS
GACCGTATGCTTCTTTGTTT+CGG 0.285549 3_3:-81357634 Msa0477190:CDS
TCCAGTGACCAGTGCCTATA+TGG 0.291143 3_3:+81354862 None:intergenic
TGATCACCTTGACATCATTT+AGG 0.295314 3_3:+81356448 None:intergenic
AGGAGGTAAGGAACAATAAA+AGG 0.298765 3_3:+81355636 None:intergenic
ATCTTGTTAGCTTTCTTAAC+AGG 0.305652 3_3:+81355372 None:intergenic
GAGGTTGTTGCTTACTTTCA+AGG 0.311089 3_3:-81357823 Msa0477190:CDS
ACAATAAAAGGAAAAGATTT+AGG 0.311629 3_3:+81355648 None:intergenic
ACCTCAGATTTCAAAATTGC+TGG 0.313915 3_3:+81356174 None:intergenic
CTGAGTCATATGAAGCTTAC+AGG 0.314147 3_3:-81356251 Msa0477190:CDS
AGATTATGTTGATTCCCTTT+TGG 0.319352 3_3:+81356991 None:intergenic
GCAAATTGGTATTCCCAAAA+TGG 0.319594 3_3:+81357797 None:intergenic
CTTGTAGGCCTTGATTTGAT+TGG 0.321359 3_3:-81355234 Msa0477190:CDS
AACACCAGTGGTGGTGAAAA+TGG 0.333264 3_3:-81358130 Msa0477190:CDS
AGGAAGGATTATGCAAAATA+AGG 0.334621 3_3:+81355761 None:intergenic
ATGAACTCTGCTCAGACATT+TGG 0.344263 3_3:+81354711 None:intergenic
GTGCCGTTGGTTGTGTTACT+TGG 0.350606 3_3:-81357987 Msa0477190:CDS
ATTTACAATGGCTCCAAGAT+TGG 0.353231 3_3:-81358190 Msa0477190:exon
CATGAGTTAAGTAAATAAAA+TGG 0.353932 3_3:-81354559 Msa0477190:three_prime_UTR
TCTGGAACAAACATGGTTAA+AGG 0.354104 3_3:+81355741 None:intergenic
CTACATTGCTCCACCAATTC+AGG 0.355815 3_3:-81357701 Msa0477190:CDS
AAACCATATGGAACCACCTT+TGG 0.363318 3_3:+81357752 None:intergenic
GCAAACTGTGCCACATTTCA+AGG 0.370258 3_3:-81356835 Msa0477190:intron
TTTGTTGCTAATTGTATTGC+TGG 0.372081 3_3:+81355027 None:intergenic
TCCATATGGTTTATGTTGCT+TGG 0.374551 3_3:-81357741 Msa0477190:CDS
GAGCATACAGTGGTTAATTA+AGG 0.378443 3_3:-81357033 Msa0477190:CDS
TTGGTTAGTAACTGTTCTTT+GGG 0.379476 3_3:-81357968 Msa0477190:CDS
ATCAAAGCCCTAGAAGAATC+TGG 0.380417 3_3:-81356549 Msa0477190:CDS
ATCTTCACAACTGAGAAATT+GGG 0.381353 3_3:+81358102 None:intergenic
TCCATATAGGCACTGGTCAC+TGG 0.387795 3_3:-81354863 Msa0477190:three_prime_UTR
AGCAACACCGCTGGCATTCA+GGG 0.390128 3_3:-81356224 Msa0477190:CDS
ATAACAGAGAAACCTTGTTT+TGG 0.396626 3_3:+81354933 None:intergenic
GGTATCTGGACTCTTCCAAT+TGG 0.403642 3_3:-81355574 Msa0477190:CDS
GGGATGACATTCTTCCTTGT+AGG 0.403705 3_3:-81355249 Msa0477190:CDS
AATTGGGAAGCTCTTATGAA+TGG 0.404164 3_3:-81355557 Msa0477190:CDS
AATGGATCCCCATTGATCAT+TGG 0.406112 3_3:+81357580 None:intergenic
GCTTGGACCAAACCTAGTTC+AGG 0.414765 3_3:+81356717 None:intergenic
GAGAACAACTGTAGAAGATA+TGG 0.416414 3_3:-81356514 Msa0477190:CDS
TTTCCAAAGGTGGTTCCATA+TGG 0.418411 3_3:-81357755 Msa0477190:CDS
TTTGGTTTCAATGGAACTGC+TGG 0.420392 3_3:-81356582 Msa0477190:CDS
TGGAACGTGAAACACACTAT+TGG 0.421234 3_3:+81355152 None:intergenic
TGCTTAAGAAAATCACGGTT+TGG 0.423971 3_3:+81356855 None:intergenic
CAACACGGGTTCAGCATCCT+TGG 0.430491 3_3:+81355132 None:intergenic
GCAAGAATGCTAAACAAGTT+AGG 0.432413 3_3:-81358035 Msa0477190:CDS
AGTATGAGGAATGTGCAAAT+TGG 0.435051 3_3:+81357783 None:intergenic
AGGTTAAGCCAATGATCAAT+GGG 0.437598 3_3:-81357588 Msa0477190:CDS
GAAGTAGAACAGCAGGTCAA+TGG 0.438411 3_3:-81356621 Msa0477190:CDS
GAAAAGGCTGGTTCGTAGAC+AGG 0.438680 3_3:-81357926 Msa0477190:CDS
AACTTCGAAAGTGCCTGAAT+TGG 0.444764 3_3:+81357688 None:intergenic
AATTTCTGCAGTGTGTCAAA+TGG 0.446405 3_3:-81357108 Msa0477190:CDS
TTCGAAAGTGCCTGAATTGG+TGG 0.450121 3_3:+81357691 None:intergenic
TCAATGGAACTGCTGGTGTT+TGG 0.451289 3_3:-81356575 Msa0477190:CDS
ACCAAGCAACATAAACCATA+TGG 0.452313 3_3:+81357740 None:intergenic
AAGTAGAACAGCAGGTCAAT+GGG 0.453067 3_3:-81356620 Msa0477190:CDS
GGATAATCCTGAACTAGGTT+TGG 0.455019 3_3:-81356724 Msa0477190:CDS
ATTGGAAAAGGCACATTATG+AGG 0.455854 3_3:+81358443 None:intergenic
ACATAGCAGATCAACCATAA+AGG 0.458153 3_3:+81355708 None:intergenic
GAATAAGACACAAACTTTCA+AGG 0.461463 3_3:+81358474 None:intergenic
AAAGGAAAAGATTTAGGTGC+AGG 0.463485 3_3:+81355654 None:intergenic
TGTTGAGTTACAGCAATTGA+AGG 0.464132 3_3:-81355418 Msa0477190:CDS
TGCTTGGTGCTATTAAGAAA+AGG 0.466972 3_3:-81357942 Msa0477190:CDS
GGAATTTGAACCAAAACCAT+TGG 0.468674 3_3:+81357520 None:intergenic
TCAACATCATCAACCTTGAA+TGG 0.468739 3_3:+81357562 None:intergenic
AAGACACCTAACATTGGAAA+AGG 0.469988 3_3:+81358431 None:intergenic
GGTCTCTAAGTGGAACCAAA+AGG 0.473118 3_3:-81357006 Msa0477190:CDS
GACATTTGGAATGGTGAGAT+AGG 0.474946 3_3:+81354725 None:intergenic
ATCTGTATGCATTTATCCAA+TGG 0.475291 3_3:-81357536 Msa0477190:CDS
AAGGTTAAGCCAATGATCAA+TGG 0.477569 3_3:-81357589 Msa0477190:CDS
CGGCATCGCTTGGTCAGGAC+TGG 0.477703 3_3:-81356966 Msa0477190:CDS
ATTCATAAGAGCTTCCCAAT+TGG 0.477893 3_3:+81355559 None:intergenic
TCTATCGGCATCGCTTGGTC+AGG 0.480004 3_3:-81356971 Msa0477190:CDS
TTACAGGATAATCCTGAACT+AGG 0.481264 3_3:-81356729 Msa0477190:intron
TCCAATGTGCAATGAGAAAG+AGG 0.482610 3_3:-81357500 Msa0477190:intron
GTCAGGACTGGATATAAAGC+TGG 0.486529 3_3:-81356954 Msa0477190:CDS
TCATCACAAGTATTCCATAT+AGG 0.488036 3_3:-81354876 Msa0477190:three_prime_UTR
ACTGTTCTTTGGGATTTGCT+TGG 0.488047 3_3:-81357958 Msa0477190:CDS
CGTGTCAGCGCACAATTGAA+TGG 0.488875 3_3:+81355090 None:intergenic
ATTAAGTTTGCTTTCTTCCA+CGG 0.493411 3_3:+81355834 None:intergenic
GAAGCATACGGTCCACGGAT+TGG 0.496178 3_3:+81357644 None:intergenic
AATAAGGTGAAGGAGTAAAA+GGG 0.501255 3_3:+81355777 None:intergenic
GATCAATGGGGATCCATTCA+AGG 0.501987 3_3:-81357575 Msa0477190:CDS
TGACACAAGCTGTGAGAAGA+AGG 0.502725 3_3:+81355322 None:intergenic
TGGATTGTCACAAAGAAAGC+TGG 0.503165 3_3:-81355537 Msa0477190:CDS
ATATTTACAGTGATCAATCA+TGG 0.503419 3_3:+81358504 None:intergenic
CAAGGAAGAATGTCATCCCT+TGG 0.504529 3_3:+81355253 None:intergenic
ATGGTGCAGATGCTGCATTT+AGG 0.506624 3_3:-81358077 Msa0477190:CDS
GTAAATAAAATGGTGTCGGC+AGG 0.506989 3_3:-81354549 None:intergenic
TCAAAGCCCTAGAAGAATCT+GGG 0.507079 3_3:-81356548 Msa0477190:CDS
AATGGGTCAAACAGAAATGA+AGG 0.507855 3_3:+81354642 None:intergenic
CTCATACTTCTGATTTCCAA+AGG 0.508694 3_3:-81357768 Msa0477190:CDS
GTATCTGGACTCTTCCAATT+GGG 0.517060 3_3:-81355573 Msa0477190:CDS
TTCAAGGTTGATGATGTTGA+AGG 0.517442 3_3:-81357559 Msa0477190:CDS
CTTAAAGCTCATCGTGCCGT+TGG 0.518642 3_3:-81358000 Msa0477190:CDS
CATAAAGGAAGTTCTGCCTC+TGG 0.523961 3_3:+81355723 None:intergenic
TCCGAGCTCACCTCAGTGGT+TGG 0.525595 3_3:-81356482 Msa0477190:CDS
ATAGTGTGTTTCACGTTCCA+AGG 0.526080 3_3:-81355149 Msa0477190:three_prime_UTR
GTCTCTAAGTGGAACCAAAA+GGG 0.527274 3_3:-81357005 Msa0477190:CDS
CTTTGGAAATCAGAAGTATG+AGG 0.527295 3_3:+81357769 None:intergenic
CAATCAAATCAAGGCCTACA+AGG 0.531066 3_3:+81355235 None:intergenic
TCCAACCACTGAGGTGAGCT+CGG 0.537202 3_3:+81356481 None:intergenic
ACCTCTTTCTCATTGCACAT+TGG 0.537709 3_3:+81357499 None:intergenic
CATAATCTATCGGCATCGCT+TGG 0.540008 3_3:-81356976 Msa0477190:CDS
ACAAAGAAGCATACGGTCCA+CGG 0.540829 3_3:+81357639 None:intergenic
AAAAGGCTGGTTCGTAGACA+GGG 0.541112 3_3:-81357925 Msa0477190:CDS
TCTGCTCTCCAATCAAATCA+AGG 0.541124 3_3:+81355226 None:intergenic
TGGTGCTATTAAGAAAAGGC+TGG 0.543425 3_3:-81357938 Msa0477190:CDS
CTGTTCTGAGAGTTTCAGAA+AGG 0.544450 3_3:+81354984 None:intergenic
TTTAACCATGTTTGTTCCAG+AGG 0.547320 3_3:-81355739 Msa0477190:CDS
CTGCAGTGTGTCAAATGGAT+TGG 0.548428 3_3:-81357103 Msa0477190:CDS
TTAAGGATTGACATGCATAC+AGG 0.551931 3_3:+81355684 None:intergenic
TTTGTTTCTTATCCAATCCG+TGG 0.553534 3_3:-81357656 Msa0477190:CDS
TATCTTCCTAAATGATGTCA+AGG 0.553615 3_3:-81356454 Msa0477190:intron
TCACAAAAGACCATCTAGCT+TGG 0.556166 3_3:+81356700 None:intergenic
GAATGCTAAACAAGTTAGGT+GGG 0.557662 3_3:-81358031 Msa0477190:CDS
GCTGGTTCGTAGACAGGGAG+TGG 0.560932 3_3:-81357920 Msa0477190:CDS
TGAATCAGTAAACGATCCCT+TGG 0.561328 3_3:+81357080 None:intergenic
TAGGTGCAGGAAGAATGTTA+AGG 0.561478 3_3:+81355667 None:intergenic
AGGGAATCAACATAATCTAT+CGG 0.563806 3_3:-81356986 Msa0477190:CDS
TGTGTCAAATGGATTGGCCA+AGG 0.564789 3_3:-81357097 Msa0477190:CDS
CAAGTATTCCATATAGGCAC+TGG 0.568611 3_3:-81354870 Msa0477190:three_prime_UTR
AGAATGCTAAACAAGTTAGG+TGG 0.573310 3_3:-81358032 Msa0477190:CDS
TGCAGATGCTGCATTTAGGC+CGG 0.573889 3_3:-81358073 Msa0477190:CDS
TCTTCACAACTGAGAAATTG+GGG 0.578230 3_3:+81358103 None:intergenic
ACAGGAAGCAGCAACACCGC+TGG 0.578674 3_3:-81356233 Msa0477190:CDS
GTATGCTTCTTTGTTTCGGG+TGG 0.578929 3_3:-81357630 Msa0477190:CDS
CTGTAAGCTTCATATGACTC+AGG 0.579123 3_3:+81356252 None:intergenic
TAGGTTTGGTCCAAGCTAGA+TGG 0.582078 3_3:-81356710 Msa0477190:CDS
ACAGTGGTTAATTAAGGCTG+AGG 0.582788 3_3:-81357027 Msa0477190:CDS
GAACAAACATGGTTAAAGGA+AGG 0.583578 3_3:+81355745 None:intergenic
TTAAGTAAATAAAATGGTGT+CGG 0.583799 3_3:-81354553 Msa0477190:three_prime_UTR
TGGATGAAGGATTCACAGAA+AGG 0.587309 3_3:-81358153 Msa0477190:CDS
CTTGTTTAGCATTCTTGCCG+CGG 0.588225 3_3:+81358040 None:intergenic
CTCTGCTCAGACATTTGGAA+TGG 0.590647 3_3:+81354716 None:intergenic
ATACTTCTGATTTCCAAAGG+TGG 0.593673 3_3:-81357765 Msa0477190:CDS
TGTGACTAAATTTAATGCAA+TGG 0.593823 3_3:-81355595 Msa0477190:CDS
TTCTGCCTCTGGAACAAACA+TGG 0.595292 3_3:+81355734 None:intergenic
GATTATGCAAAATAAGGTGA+AGG 0.598839 3_3:+81355767 None:intergenic
CAGTTTGCTTAAGAAAATCA+CGG 0.601825 3_3:+81356850 None:intergenic
AGGTGAAGGAGTAAAAGGGA+AGG 0.604134 3_3:+81355781 None:intergenic
CTTCACTTAACCTTGAAATG+TGG 0.610582 3_3:+81356825 None:intergenic
CAAAGCCCTAGAAGAATCTG+GGG 0.611476 3_3:-81356547 Msa0477190:CDS
CACAGAGGAGCTTCTGACAG+TGG 0.622297 3_3:-81355444 Msa0477190:CDS
TAACCAAGTAACACAACCAA+CGG 0.623439 3_3:+81357984 None:intergenic
GTGTCAAATGGATTGGCCAA+GGG 0.623875 3_3:-81357096 Msa0477190:CDS
TACATATAGTTGATTCAACA+CGG 0.632486 3_3:+81355117 None:intergenic
ACCCGAAACAAAGAAGCATA+CGG 0.633312 3_3:+81357632 None:intergenic
TTAAGGCTGAGGTCTCTAAG+TGG 0.635565 3_3:-81357016 Msa0477190:CDS
TGCAACTTATAGTGTGTGTG+TGG 0.638443 3_3:+81355052 None:intergenic
ACAGAAAGGAACACCAGTGG+TGG 0.639233 3_3:-81358139 Msa0477190:CDS
ATTAAATTTAGTCACAGACA+TGG 0.642279 3_3:+81355602 None:intergenic
TGTGAAACTGTGATTTACAA+TGG 0.643682 3_3:-81358202 Msa0477190:exon
GACAACAAAGAGTAAAGACA+TGG 0.646506 3_3:+81358331 None:intergenic
AGAAGTCTGTTATCTGCACA+AGG 0.657709 3_3:-81355300 Msa0477190:CDS
GCTGTCCGAGCTCACCTCAG+TGG 0.658750 3_3:-81356486 Msa0477190:CDS
ACATATAGTTGATTCAACAC+GGG 0.660206 3_3:+81355118 None:intergenic
GCATAGGCCCTGAATGCCAG+CGG 0.668252 3_3:+81356217 None:intergenic
TGATTAATCAGCAGAAGATG+TGG 0.669212 3_3:+81355176 None:intergenic
TTGTCACAAAGAAAGCTGGA+AGG 0.675157 3_3:-81355533 Msa0477190:CDS
ACCAGTGGTGGTGAAAATGG+AGG 0.695679 3_3:-81358127 Msa0477190:CDS
AGTAGAACAGCAGGTCAATG+GGG 0.717208 3_3:-81356619 Msa0477190:CDS
TTCACAGAAAGGAACACCAG+TGG 0.722164 3_3:-81358142 Msa0477190:CDS
GAACAACAAAAGATTCACAG+AGG 0.731952 3_3:-81355459 Msa0477190:CDS
AAAGCCCTAGAAGAATCTGG+GGG 0.740097 3_3:-81356546 Msa0477190:CDS
AGATAAATTTCCAACCACTG+AGG 0.740352 3_3:+81356472 None:intergenic
CTGATGATGAGAGCATACAG+TGG 0.744429 3_3:-81357043 Msa0477190:CDS
TTTAACTGCAGGTGTCACCG+TGG 0.746704 3_3:-81355851 Msa0477190:intron
GGTTAAGCCAATGATCAATG+GGG 0.761083 3_3:-81357587 Msa0477190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTTTTTGTTAATTTCTT+TGG - chr3_3:81355226-81355245 Msa0477190:CDS 15.0%
!! AAAACAAAGAAAAAACAAAT+GGG + chr3_3:81358464-81358483 None:intergenic 15.0%
!! AAAAACAAAGAAAAAACAAA+TGG + chr3_3:81358465-81358484 None:intergenic 15.0%
!! CTTAATTGTGTTTAAATTTC+AGG - chr3_3:81356797-81356816 Msa0477190:intron 20.0%
!!! AATAAAATTTAAGACCCTTT+AGG + chr3_3:81357217-81357236 None:intergenic 20.0%
!!! CTTAAATTTTATTTAACTGC+AGG - chr3_3:81357224-81357243 Msa0477190:intron 20.0%
!! ACAATAAAAGGAAAAGATTT+AGG + chr3_3:81357441-81357460 None:intergenic 20.0%
!!! TTATTTTAAGTACCAAAACA+AGG - chr3_3:81358141-81358160 Msa0477190:CDS 20.0%
!!! GTTTTCTTTATTTTGTTCTT+TGG - chr3_3:81358305-81358324 Msa0477190:five_prime_UTR 20.0%
!! CATGAGTTAAGTAAATAAAA+TGG - chr3_3:81358527-81358546 Msa0477190:five_prime_UTR 20.0%
!! TTAAGTAAATAAAATGGTGT+CGG - chr3_3:81358533-81358552 Msa0477190:five_prime_UTR 20.0%
! ATATTTACAGTGATCAATCA+TGG + chr3_3:81354585-81354604 None:intergenic 25.0%
! ACTATTACATTGAAACTTAC+TGG + chr3_3:81355675-81355694 None:intergenic 25.0%
! AGATTGCAGAAAATTCTTAA+GGG + chr3_3:81355806-81355825 None:intergenic 25.0%
! TCAATGACATTGACAAAATA+TGG - chr3_3:81355899-81355918 Msa0477190:intron 25.0%
!!! GGAAGCATAAATTTATGTTT+TGG + chr3_3:81356440-81356459 None:intergenic 25.0%
!! CTTAATTTCTTTGGTTTCAA+TGG - chr3_3:81356495-81356514 Msa0477190:CDS 25.0%
! TATTATGCACTATGAAAGAA+AGG + chr3_3:81356701-81356720 None:intergenic 25.0%
! AAATTTAAGAGAGTTCTTCA+GGG + chr3_3:81356777-81356796 None:intergenic 25.0%
!!! TATAAAGTGTTGTCAAATAG+CGG - chr3_3:81357072-81357091 Msa0477190:CDS 25.0%
! TACTGAGTATTTGCAATTAT+TGG - chr3_3:81357176-81357195 Msa0477190:intron 25.0%
! TTATATTATGTACTGCCTAA+AGG - chr3_3:81357199-81357218 Msa0477190:intron 25.0%
! TATATTATGTACTGCCTAAA+GGG - chr3_3:81357200-81357219 Msa0477190:intron 25.0%
! ATTAAATTTAGTCACAGACA+TGG + chr3_3:81357487-81357506 None:intergenic 25.0%
! TGTGACTAAATTTAATGCAA+TGG - chr3_3:81357491-81357510 Msa0477190:intron 25.0%
! AAATTTAATGCAATGGTATC+TGG - chr3_3:81357498-81357517 Msa0477190:intron 25.0%
!! ATATAAAAAAGAGCTGACTT+TGG - chr3_3:81357737-81357756 Msa0477190:CDS 25.0%
!! TACATATAGTTGATTCAACA+CGG + chr3_3:81357972-81357991 None:intergenic 25.0%
! GAATGAATGAAAAATCGATA+GGG + chr3_3:81358183-81358202 None:intergenic 25.0%
! TGAATGAATGAAAAATCGAT+AGG + chr3_3:81358184-81358203 None:intergenic 25.0%
!! TTTTGTTCTTTGGTTGTATT+TGG - chr3_3:81358315-81358334 Msa0477190:five_prime_UTR 25.0%
! TTTGTTCTTTGGTTGTATTT+GGG - chr3_3:81358316-81358335 Msa0477190:five_prime_UTR 25.0%
!!! CTTTGTTTTTTGTTGTTACT+TGG - chr3_3:81358475-81358494 Msa0477190:five_prime_UTR 25.0%
GAATAAGACACAAACTTTCA+AGG + chr3_3:81354615-81354634 None:intergenic 30.0%
!! TTTTTGAAGACACCTAACAT+TGG + chr3_3:81354664-81354683 None:intergenic 30.0%
! AGATTCGTAGTTGATGTTAT+TGG + chr3_3:81354710-81354729 None:intergenic 30.0%
TGTGAAACTGTGATTTACAA+TGG - chr3_3:81354884-81354903 Msa0477190:three_prime_UTR 30.0%
CAACTTGAAAAATCCAATCT+TGG + chr3_3:81354912-81354931 None:intergenic 30.0%
!!! CAAGATTGGATTTTTCAAGT+TGG - chr3_3:81354910-81354929 Msa0477190:three_prime_UTR 30.0%
ATCTTCACAACTGAGAAATT+GGG + chr3_3:81354987-81355006 None:intergenic 30.0%
AATCTTCACAACTGAGAAAT+TGG + chr3_3:81354988-81355007 None:intergenic 30.0%
TTCTCAGTTGTGAAGATTAA+TGG - chr3_3:81354990-81355009 Msa0477190:three_prime_UTR 30.0%
TTGGTTAGTAACTGTTCTTT+GGG - chr3_3:81355118-81355137 Msa0477190:three_prime_UTR 30.0%
! GTTAATTTCTTTGGCTGTTT+TGG - chr3_3:81355235-81355254 Msa0477190:CDS 30.0%
!! GTTTTGGATCAAATTCAAGA+AGG - chr3_3:81355478-81355497 Msa0477190:CDS 30.0%
ATCTGTATGCATTTATCCAA+TGG - chr3_3:81355550-81355569 Msa0477190:CDS 30.0%
! ATGCATTTATCCAATGGTTT+TGG - chr3_3:81355556-81355575 Msa0477190:CDS 30.0%
TAAAAACCTCATAGCAAAAC+TGG + chr3_3:81355634-81355653 None:intergenic 30.0%
GAGATTGCAGAAAATTCTTA+AGG + chr3_3:81355807-81355826 None:intergenic 30.0%
TAAAACAACACAGCATCAAA+TGG + chr3_3:81355855-81355874 None:intergenic 30.0%
!!! AGATTATGTTGATTCCCTTT+TGG + chr3_3:81356098-81356117 None:intergenic 30.0%
AGGGAATCAACATAATCTAT+CGG - chr3_3:81356100-81356119 Msa0477190:intron 30.0%
! CAGTTTGCTTAAGAAAATCA+CGG + chr3_3:81356239-81356258 None:intergenic 30.0%
!! TGCAAATGACTTGTTATTAC+AGG - chr3_3:81356341-81356360 Msa0477190:intron 30.0%
TATCTTCCTAAATGATGTCA+AGG - chr3_3:81356632-81356651 Msa0477190:CDS 30.0%
GAAATTTAAGAGAGTTCTTC+AGG + chr3_3:81356778-81356797 None:intergenic 30.0%
! ATTAAGTTTGCTTTCTTCCA+CGG + chr3_3:81357255-81357274 None:intergenic 30.0%
!!! TTCTGCTATTTTTTCTGCTA+AGG - chr3_3:81357274-81357293 Msa0477190:intron 30.0%
AATAAGGTGAAGGAGTAAAA+GGG + chr3_3:81357312-81357331 None:intergenic 30.0%
AAATAAGGTGAAGGAGTAAA+AGG + chr3_3:81357313-81357332 None:intergenic 30.0%
GATTATGCAAAATAAGGTGA+AGG + chr3_3:81357322-81357341 None:intergenic 30.0%
AGGAAGGATTATGCAAAATA+AGG + chr3_3:81357328-81357347 None:intergenic 30.0%
!!! CTTTTGTTGTTCTATTGACT+CGG + chr3_3:81357619-81357638 None:intergenic 30.0%
!! ATCTTGTTAGCTTTCTTAAC+AGG + chr3_3:81357717-81357736 None:intergenic 30.0%
!! TTCTACTTTCTGTTTTTCCA+AGG - chr3_3:81357816-81357835 Msa0477190:CDS 30.0%
!! TCTACTTTCTGTTTTTCCAA+GGG - chr3_3:81357817-81357836 Msa0477190:CDS 30.0%
! ACATATAGTTGATTCAACAC+GGG + chr3_3:81357971-81357990 None:intergenic 30.0%
TTTGTTGCTAATTGTATTGC+TGG + chr3_3:81358062-81358081 None:intergenic 30.0%
!! ATAACAGAGAAACCTTGTTT+TGG + chr3_3:81358156-81358175 None:intergenic 30.0%
TCATCACAAGTATTCCATAT+AGG - chr3_3:81358210-81358229 Msa0477190:five_prime_UTR 30.0%
! ATTGGAAAAGGCACATTATG+AGG + chr3_3:81354646-81354665 None:intergenic 35.0%
! AATGTGCCTTTTCCAATGTT+AGG - chr3_3:81354649-81354668 Msa0477190:three_prime_UTR 35.0%
AAGACACCTAACATTGGAAA+AGG + chr3_3:81354658-81354677 None:intergenic 35.0%
GACAACAAAGAGTAAAGACA+TGG + chr3_3:81354758-81354777 None:intergenic 35.0%
ATTTACAATGGCTCCAAGAT+TGG - chr3_3:81354896-81354915 Msa0477190:three_prime_UTR 35.0%
!!! GATTGGATTTTTCAAGTTGG+TGG - chr3_3:81354913-81354932 Msa0477190:three_prime_UTR 35.0%
!!! TTTTTCAAGTTGGTGGATGA+AGG - chr3_3:81354920-81354939 Msa0477190:three_prime_UTR 35.0%
TCTTCACAACTGAGAAATTG+GGG + chr3_3:81354986-81355005 None:intergenic 35.0%
GCAAGAATGCTAAACAAGTT+AGG - chr3_3:81355051-81355070 Msa0477190:three_prime_UTR 35.0%
AGAATGCTAAACAAGTTAGG+TGG - chr3_3:81355054-81355073 Msa0477190:three_prime_UTR 35.0%
GAATGCTAAACAAGTTAGGT+GGG - chr3_3:81355055-81355074 Msa0477190:three_prime_UTR 35.0%
TAACCAAGTAACACAACCAA+CGG + chr3_3:81355105-81355124 None:intergenic 35.0%
CTTGGTTAGTAACTGTTCTT+TGG - chr3_3:81355117-81355136 Msa0477190:three_prime_UTR 35.0%
!! TGCTTGGTGCTATTAAGAAA+AGG - chr3_3:81355144-81355163 Msa0477190:three_prime_UTR 35.0%
!! TACTTTCAAGGTTGCCATTT+TGG - chr3_3:81355275-81355294 Msa0477190:CDS 35.0%
!!! ACTTTCAAGGTTGCCATTTT+GGG - chr3_3:81355276-81355295 Msa0477190:CDS 35.0%
GCAAATTGGTATTCCCAAAA+TGG + chr3_3:81355292-81355311 None:intergenic 35.0%
AGTATGAGGAATGTGCAAAT+TGG + chr3_3:81355306-81355325 None:intergenic 35.0%
! CTTTGGAAATCAGAAGTATG+AGG + chr3_3:81355320-81355339 None:intergenic 35.0%
CTCATACTTCTGATTTCCAA+AGG - chr3_3:81355318-81355337 Msa0477190:CDS 35.0%
ATACTTCTGATTTCCAAAGG+TGG - chr3_3:81355321-81355340 Msa0477190:CDS 35.0%
TCCATATGGTTTATGTTGCT+TGG - chr3_3:81355345-81355364 Msa0477190:CDS 35.0%
ACCAAGCAACATAAACCATA+TGG + chr3_3:81355349-81355368 None:intergenic 35.0%
! TTTGTTTCTTATCCAATCCG+TGG - chr3_3:81355430-81355449 Msa0477190:CDS 35.0%
AAGGTTAAGCCAATGATCAA+TGG - chr3_3:81355497-81355516 Msa0477190:CDS 35.0%
AGGTTAAGCCAATGATCAAT+GGG - chr3_3:81355498-81355517 Msa0477190:CDS 35.0%
TCAACATCATCAACCTTGAA+TGG + chr3_3:81355527-81355546 None:intergenic 35.0%
!! TTCAAGGTTGATGATGTTGA+AGG - chr3_3:81355527-81355546 Msa0477190:CDS 35.0%
GGAATTTGAACCAAAACCAT+TGG + chr3_3:81355569-81355588 None:intergenic 35.0%
AAAACTGGAATGCAGAAGTT+CGG + chr3_3:81355619-81355638 None:intergenic 35.0%
! TGCACAAATTGACTTTATGC+AGG - chr3_3:81355942-81355961 Msa0477190:intron 35.0%
AATTTCTGCAGTGTGTCAAA+TGG - chr3_3:81355978-81355997 Msa0477190:intron 35.0%
GAGCATACAGTGGTTAATTA+AGG - chr3_3:81356053-81356072 Msa0477190:intron 35.0%
TGCTTAAGAAAATCACGGTT+TGG + chr3_3:81356234-81356253 None:intergenic 35.0%
CTTCACTTAACCTTGAAATG+TGG + chr3_3:81356264-81356283 None:intergenic 35.0%
TTACAGGATAATCCTGAACT+AGG - chr3_3:81356357-81356376 Msa0477190:intron 35.0%
! TAGATGGTCTTTTGTGAACA+AGG - chr3_3:81356392-81356411 Msa0477190:intron 35.0%
! GGGGTGTTTCTTAATTTCTT+TGG - chr3_3:81356486-81356505 Msa0477190:CDS 35.0%
GAGAACAACTGTAGAAGATA+TGG - chr3_3:81356572-81356591 Msa0477190:CDS 35.0%
AGATAAATTTCCAACCACTG+AGG + chr3_3:81356617-81356636 None:intergenic 35.0%
TGATCACCTTGACATCATTT+AGG + chr3_3:81356641-81356660 None:intergenic 35.0%
ACAGAAGTTCGATTTCATGA+AGG + chr3_3:81356739-81356758 None:intergenic 35.0%
!!! TCCAGCAATTTTGAAATCTG+AGG - chr3_3:81356911-81356930 Msa0477190:CDS 35.0%
ACCTCAGATTTCAAAATTGC+TGG + chr3_3:81356915-81356934 None:intergenic 35.0%
TGAGCAATGTTGTCAAATAG+CGG - chr3_3:81356992-81357011 Msa0477190:CDS 35.0%
TTGCAGATCGCAAAAAATAG+TGG + chr3_3:81357143-81357162 None:intergenic 35.0%
GAACAAACATGGTTAAAGGA+AGG + chr3_3:81357344-81357363 None:intergenic 35.0%
! TCTGGAACAAACATGGTTAA+AGG + chr3_3:81357348-81357367 None:intergenic 35.0%
TTTAACCATGTTTGTTCCAG+AGG - chr3_3:81357347-81357366 Msa0477190:intron 35.0%
ACATAGCAGATCAACCATAA+AGG + chr3_3:81357381-81357400 None:intergenic 35.0%
TTAAGGATTGACATGCATAC+AGG + chr3_3:81357405-81357424 None:intergenic 35.0%
AAAGGAAAAGATTTAGGTGC+AGG + chr3_3:81357435-81357454 None:intergenic 35.0%
AGGAGGTAAGGAACAATAAA+AGG + chr3_3:81357453-81357472 None:intergenic 35.0%
! GTGTTTTCAAAAAGGAGGTA+AGG + chr3_3:81357465-81357484 None:intergenic 35.0%
! ACATGGTGTTTTCAAAAAGG+AGG + chr3_3:81357470-81357489 None:intergenic 35.0%
! CAGACATGGTGTTTTCAAAA+AGG + chr3_3:81357473-81357492 None:intergenic 35.0%
ATTCATAAGAGCTTCCCAAT+TGG + chr3_3:81357530-81357549 None:intergenic 35.0%
AATTGGGAAGCTCTTATGAA+TGG - chr3_3:81357529-81357548 Msa0477190:CDS 35.0%
GAACAACAAAAGATTCACAG+AGG - chr3_3:81357627-81357646 Msa0477190:CDS 35.0%
TGTTGAGTTACAGCAATTGA+AGG - chr3_3:81357668-81357687 Msa0477190:CDS 35.0%
! TTAGCTTTCTTAACAGGTTC+TGG + chr3_3:81357711-81357730 None:intergenic 35.0%
TGATTAATCAGCAGAAGATG+TGG + chr3_3:81357913-81357932 None:intergenic 35.0%
AATGGGTCAAACAGAAATGA+AGG + chr3_3:81358447-81358466 None:intergenic 35.0%
GGAACAAAAATGCAGAGTGA+GGG + chr3_3:81354689-81354708 None:intergenic 40.0%
TGGAACAAAAATGCAGAGTG+AGG + chr3_3:81354690-81354709 None:intergenic 40.0%
TGGATGAAGGATTCACAGAA+AGG - chr3_3:81354933-81354952 Msa0477190:three_prime_UTR 40.0%
!! ACTGTTCTTTGGGATTTGCT+TGG - chr3_3:81355128-81355147 Msa0477190:three_prime_UTR 40.0%
!! TGGTGCTATTAAGAAAAGGC+TGG - chr3_3:81355148-81355167 Msa0477190:three_prime_UTR 40.0%
!!! TTTGGCTGTTTTGGCTTTTG+AGG - chr3_3:81355244-81355263 Msa0477190:CDS 40.0%
! GAGGTTGTTGCTTACTTTCA+AGG - chr3_3:81355263-81355282 Msa0477190:CDS 40.0%
TTTCCAAAGGTGGTTCCATA+TGG - chr3_3:81355331-81355350 Msa0477190:CDS 40.0%
AAACCATATGGAACCACCTT+TGG + chr3_3:81355337-81355356 None:intergenic 40.0%
!! AACTTCGAAAGTGCCTGAAT+TGG + chr3_3:81355401-81355420 None:intergenic 40.0%
! GACCGTATGCTTCTTTGTTT+CGG - chr3_3:81355452-81355471 Msa0477190:CDS 40.0%
! ACCGTATGCTTCTTTGTTTC+GGG - chr3_3:81355453-81355472 Msa0477190:CDS 40.0%
! ACCCGAAACAAAGAAGCATA+CGG + chr3_3:81355457-81355476 None:intergenic 40.0%
!! TTCTTTGTTTCGGGTGGTTT+TGG - chr3_3:81355462-81355481 Msa0477190:CDS 40.0%
GGTTAAGCCAATGATCAATG+GGG - chr3_3:81355499-81355518 Msa0477190:CDS 40.0%
AATGGATCCCCATTGATCAT+TGG + chr3_3:81355509-81355528 None:intergenic 40.0%
TCCAATGTGCAATGAGAAAG+AGG - chr3_3:81355586-81355605 Msa0477190:CDS 40.0%
ACCTCTTTCTCATTGCACAT+TGG + chr3_3:81355590-81355609 None:intergenic 40.0%
!! TGCATTCCAGTTTTGCTATG+AGG - chr3_3:81355625-81355644 Msa0477190:CDS 40.0%
! GCATTTGTGTTTCTGAGTTC+AGG + chr3_3:81355829-81355848 None:intergenic 40.0%
TGAATCAGTAAACGATCCCT+TGG + chr3_3:81356009-81356028 None:intergenic 40.0%
ACAGTGGTTAATTAAGGCTG+AGG - chr3_3:81356059-81356078 Msa0477190:intron 40.0%
GTCTCTAAGTGGAACCAAAA+GGG - chr3_3:81356081-81356100 Msa0477190:intron 40.0%
GGATAATCCTGAACTAGGTT+TGG - chr3_3:81356362-81356381 Msa0477190:intron 40.0%
TCACAAAAGACCATCTAGCT+TGG + chr3_3:81356389-81356408 None:intergenic 40.0%
CCTGCTGTTCTACTTCAAAA+TGG + chr3_3:81356461-81356480 None:intergenic 40.0%
!!! CCATTTTGAAGTAGAACAGC+AGG - chr3_3:81356458-81356477 Msa0477190:CDS 40.0%
AAGTAGAACAGCAGGTCAAT+GGG - chr3_3:81356466-81356485 Msa0477190:CDS 40.0%
! TTTGGTTTCAATGGAACTGC+TGG - chr3_3:81356504-81356523 Msa0477190:CDS 40.0%
ATCAAAGCCCTAGAAGAATC+TGG - chr3_3:81356537-81356556 Msa0477190:CDS 40.0%
TCAAAGCCCTAGAAGAATCT+GGG - chr3_3:81356538-81356557 Msa0477190:CDS 40.0%
CTGTAAGCTTCATATGACTC+AGG + chr3_3:81356837-81356856 None:intergenic 40.0%
CTGAGTCATATGAAGCTTAC+AGG - chr3_3:81356835-81356854 Msa0477190:intron 40.0%
CACAGCCTAAAAAGTTGCAT+AGG + chr3_3:81356888-81356907 None:intergenic 40.0%
TATGTTGTAGAGCTATAGCG+CGG + chr3_3:81357104-81357123 None:intergenic 40.0%
TAGGTGCAGGAAGAATGTTA+AGG + chr3_3:81357422-81357441 None:intergenic 40.0%
GTATCTGGACTCTTCCAATT+GGG - chr3_3:81357513-81357532 Msa0477190:CDS 40.0%
TGGATTGTCACAAAGAAAGC+TGG - chr3_3:81357549-81357568 Msa0477190:CDS 40.0%
TTGTCACAAAGAAAGCTGGA+AGG - chr3_3:81357553-81357572 Msa0477190:CDS 40.0%
AGAAGTCTGTTATCTGCACA+AGG - chr3_3:81357786-81357805 Msa0477190:CDS 40.0%
CAATCAAATCAAGGCCTACA+AGG + chr3_3:81357854-81357873 None:intergenic 40.0%
! CTTGTAGGCCTTGATTTGAT+TGG - chr3_3:81357852-81357871 Msa0477190:CDS 40.0%
TCTGCTCTCCAATCAAATCA+AGG + chr3_3:81357863-81357882 None:intergenic 40.0%
TGGAACGTGAAACACACTAT+TGG + chr3_3:81357937-81357956 None:intergenic 40.0%
ATAGTGTGTTTCACGTTCCA+AGG - chr3_3:81357937-81357956 Msa0477190:CDS 40.0%
TGCAACTTATAGTGTGTGTG+TGG + chr3_3:81358037-81358056 None:intergenic 40.0%
!! CTGTTCTGAGAGTTTCAGAA+AGG + chr3_3:81358105-81358124 None:intergenic 40.0%
CAAGTATTCCATATAGGCAC+TGG - chr3_3:81358216-81358235 Msa0477190:five_prime_UTR 40.0%
! GACATTTGGAATGGTGAGAT+AGG + chr3_3:81358364-81358383 None:intergenic 40.0%
ATGAACTCTGCTCAGACATT+TGG + chr3_3:81358378-81358397 None:intergenic 40.0%
TTCACAGAAAGGAACACCAG+TGG - chr3_3:81354944-81354963 Msa0477190:three_prime_UTR 45.0%
! AACACCAGTGGTGGTGAAAA+TGG - chr3_3:81354956-81354975 Msa0477190:three_prime_UTR 45.0%
ATGGTGCAGATGCTGCATTT+AGG - chr3_3:81355009-81355028 Msa0477190:three_prime_UTR 45.0%
! TTGCCGCGGTTTTTATCTAC+CGG + chr3_3:81355035-81355054 None:intergenic 45.0%
CTTGTTTAGCATTCTTGCCG+CGG + chr3_3:81355049-81355068 None:intergenic 45.0%
!! AAAAGGCTGGTTCGTAGACA+GGG - chr3_3:81355161-81355180 Msa0477190:three_prime_UTR 45.0%
AGGGAGTGGCTGTTGAAAAA+GGG - chr3_3:81355180-81355199 Msa0477190:three_prime_UTR 45.0%
CTACATTGCTCCACCAATTC+AGG - chr3_3:81355385-81355404 Msa0477190:CDS 45.0%
!! TTCGAAAGTGCCTGAATTGG+TGG + chr3_3:81355398-81355417 None:intergenic 45.0%
! ACAAAGAAGCATACGGTCCA+CGG + chr3_3:81355450-81355469 None:intergenic 45.0%
! GTATGCTTCTTTGTTTCGGG+TGG - chr3_3:81355456-81355475 Msa0477190:CDS 45.0%
GATCAATGGGGATCCATTCA+AGG - chr3_3:81355511-81355530 Msa0477190:CDS 45.0%
CTGCAGTGTGTCAAATGGAT+TGG - chr3_3:81355983-81356002 Msa0477190:intron 45.0%
!! TGTGTCAAATGGATTGGCCA+AGG - chr3_3:81355989-81356008 Msa0477190:intron 45.0%
!! GTGTCAAATGGATTGGCCAA+GGG - chr3_3:81355990-81356009 Msa0477190:intron 45.0%
! CTGATGATGAGAGCATACAG+TGG - chr3_3:81356043-81356062 Msa0477190:intron 45.0%
TTAAGGCTGAGGTCTCTAAG+TGG - chr3_3:81356070-81356089 Msa0477190:intron 45.0%
GGTCTCTAAGTGGAACCAAA+AGG - chr3_3:81356080-81356099 Msa0477190:intron 45.0%
CATAATCTATCGGCATCGCT+TGG - chr3_3:81356110-81356129 Msa0477190:intron 45.0%
GTCAGGACTGGATATAAAGC+TGG - chr3_3:81356132-81356151 Msa0477190:intron 45.0%
GCAAACTGTGCCACATTTCA+AGG - chr3_3:81356251-81356270 Msa0477190:CDS 45.0%
! TAGGTTTGGTCCAAGCTAGA+TGG - chr3_3:81356376-81356395 Msa0477190:intron 45.0%
GAAGTAGAACAGCAGGTCAA+TGG - chr3_3:81356465-81356484 Msa0477190:CDS 45.0%
AGTAGAACAGCAGGTCAATG+GGG - chr3_3:81356467-81356486 Msa0477190:CDS 45.0%
TCAATGGAACTGCTGGTGTT+TGG - chr3_3:81356511-81356530 Msa0477190:CDS 45.0%
CAAAGCCCTAGAAGAATCTG+GGG - chr3_3:81356539-81356558 Msa0477190:CDS 45.0%
AAAGCCCTAGAAGAATCTGG+GGG - chr3_3:81356540-81356559 Msa0477190:CDS 45.0%
GAGAGTTCTTCAGGGATCTT+TGG + chr3_3:81356769-81356788 None:intergenic 45.0%
! CAGGGCCTATGCAACTTTTT+AGG - chr3_3:81356880-81356899 Msa0477190:CDS 45.0%
AGGTGAAGGAGTAAAAGGGA+AGG + chr3_3:81357308-81357327 None:intergenic 45.0%
!! TTCTGCCTCTGGAACAAACA+TGG + chr3_3:81357355-81357374 None:intergenic 45.0%
CATAAAGGAAGTTCTGCCTC+TGG + chr3_3:81357366-81357385 None:intergenic 45.0%
CAGAGGCAGAACTTCCTTTA+TGG - chr3_3:81357364-81357383 Msa0477190:intron 45.0%
GGTATCTGGACTCTTCCAAT+TGG - chr3_3:81357512-81357531 Msa0477190:CDS 45.0%
TGACACAAGCTGTGAGAAGA+AGG + chr3_3:81357767-81357786 None:intergenic 45.0%
CAAGGAAGAATGTCATCCCT+TGG + chr3_3:81357836-81357855 None:intergenic 45.0%
GGGATGACATTCTTCCTTGT+AGG - chr3_3:81357837-81357856 Msa0477190:CDS 45.0%
CTCTGCTCAGACATTTGGAA+TGG + chr3_3:81358373-81358392 None:intergenic 45.0%
ACAGAAAGGAACACCAGTGG+TGG - chr3_3:81354947-81354966 Msa0477190:three_prime_UTR 50.0%
! ACCAGTGGTGGTGAAAATGG+AGG - chr3_3:81354959-81354978 Msa0477190:three_prime_UTR 50.0%
! TCCTCCATTTTCACCACCAC+TGG + chr3_3:81354963-81354982 None:intergenic 50.0%
TGCAGATGCTGCATTTAGGC+CGG - chr3_3:81355013-81355032 Msa0477190:three_prime_UTR 50.0%
AGGCCGGTAGATAAAAACCG+CGG - chr3_3:81355029-81355048 Msa0477190:three_prime_UTR 50.0%
CTTAAAGCTCATCGTGCCGT+TGG - chr3_3:81355086-81355105 Msa0477190:three_prime_UTR 50.0%
! GTGCCGTTGGTTGTGTTACT+TGG - chr3_3:81355099-81355118 Msa0477190:three_prime_UTR 50.0%
!! GAAAAGGCTGGTTCGTAGAC+AGG - chr3_3:81355160-81355179 Msa0477190:three_prime_UTR 50.0%
CAGGGAGTGGCTGTTGAAAA+AGG - chr3_3:81355179-81355198 Msa0477190:three_prime_UTR 50.0%
GCTTGGACCAAACCTAGTTC+AGG + chr3_3:81356372-81356391 None:intergenic 50.0%
! TTTAACTGCAGGTGTCACCG+TGG - chr3_3:81357235-81357254 Msa0477190:intron 50.0%
CGTGTCAGCGCACAATTGAA+TGG + chr3_3:81357999-81358018 None:intergenic 50.0%
!! TCCATATAGGCACTGGTCAC+TGG - chr3_3:81358223-81358242 Msa0477190:five_prime_UTR 50.0%
TCCAGTGACCAGTGCCTATA+TGG + chr3_3:81358227-81358246 None:intergenic 50.0%
GAAGCATACGGTCCACGGAT+TGG + chr3_3:81355445-81355464 None:intergenic 55.0%
TCTATCGGCATCGCTTGGTC+AGG - chr3_3:81356115-81356134 Msa0477190:intron 55.0%
CCAGCCCCCAGATTCTTCTA+GGG + chr3_3:81356547-81356566 None:intergenic 55.0%
TCCAACCACTGAGGTGAGCT+CGG + chr3_3:81356608-81356627 None:intergenic 55.0%
AGCAACACCGCTGGCATTCA+GGG - chr3_3:81356862-81356881 Msa0477190:CDS 55.0%
CACAGAGGAGCTTCTGACAG+TGG - chr3_3:81357642-81357661 Msa0477190:CDS 55.0%
CAACACGGGTTCAGCATCCT+TGG + chr3_3:81357957-81357976 None:intergenic 55.0%
!! GCTGGTTCGTAGACAGGGAG+TGG - chr3_3:81355166-81355185 Msa0477190:three_prime_UTR 60.0%
CCCTAGAAGAATCTGGGGGC+TGG - chr3_3:81356544-81356563 Msa0477190:CDS 60.0%
GCCAGCCCCCAGATTCTTCT+AGG + chr3_3:81356548-81356567 None:intergenic 60.0%
! AGAAGAATCTGGGGGCTGGC+TGG - chr3_3:81356548-81356567 Msa0477190:CDS 60.0%
TCCGAGCTCACCTCAGTGGT+TGG - chr3_3:81356604-81356623 Msa0477190:CDS 60.0%
ACAGGAAGCAGCAACACCGC+TGG - chr3_3:81356853-81356872 Msa0477190:CDS 60.0%
CAGCAACACCGCTGGCATTC+AGG - chr3_3:81356861-81356880 Msa0477190:CDS 60.0%
GCATAGGCCCTGAATGCCAG+CGG + chr3_3:81356872-81356891 None:intergenic 60.0%
CGGCATCGCTTGGTCAGGAC+TGG - chr3_3:81356120-81356139 Msa0477190:intron 65.0%
GCTGTCCGAGCTCACCTCAG+TGG - chr3_3:81356600-81356619 Msa0477190:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 81354552 81358556 81354552 ID=Msa0477190;Name=Msa0477190
chr3_3 mRNA 81354552 81358556 81354552 ID=Msa0477190-mRNA-1;Parent=Msa0477190;Name=Msa0477190-mRNA-1;_AED=0.05;_eAED=0.05;_QI=351|1|1|1|1|1|5|666|687
chr3_3 exon 81354552 81355862 81354552 ID=Msa0477190-mRNA-1:exon:22414;Parent=Msa0477190-mRNA-1
chr3_3 exon 81356176 81356289 81356176 ID=Msa0477190-mRNA-1:exon:22413;Parent=Msa0477190-mRNA-1
chr3_3 exon 81356455 81356745 81356455 ID=Msa0477190-mRNA-1:exon:22412;Parent=Msa0477190-mRNA-1
chr3_3 exon 81356836 81357144 81356836 ID=Msa0477190-mRNA-1:exon:22411;Parent=Msa0477190-mRNA-1
chr3_3 exon 81357501 81358556 81357501 ID=Msa0477190-mRNA-1:exon:22410;Parent=Msa0477190-mRNA-1
chr3_3 five_prime_UTR 81358206 81358556 81358206 ID=Msa0477190-mRNA-1:five_prime_utr;Parent=Msa0477190-mRNA-1
chr3_3 CDS 81357501 81358205 81357501 ID=Msa0477190-mRNA-1:cds;Parent=Msa0477190-mRNA-1
chr3_3 CDS 81356836 81357144 81356836 ID=Msa0477190-mRNA-1:cds;Parent=Msa0477190-mRNA-1
chr3_3 CDS 81356455 81356745 81356455 ID=Msa0477190-mRNA-1:cds;Parent=Msa0477190-mRNA-1
chr3_3 CDS 81356176 81356289 81356176 ID=Msa0477190-mRNA-1:cds;Parent=Msa0477190-mRNA-1
chr3_3 CDS 81355218 81355862 81355218 ID=Msa0477190-mRNA-1:cds;Parent=Msa0477190-mRNA-1
chr3_3 three_prime_UTR 81354552 81355217 81354552 ID=Msa0477190-mRNA-1:three_prime_utr;Parent=Msa0477190-mRNA-1
Gene Sequence

>Msa0477190

ATGGCTCCAAGATTGGATTTTTCAAGTTGGTGGATGAAGGATTCACAGAAAGGAACACCAGTGGTGGTGAAAATGGAGGACCCCAATTTCTCAGTTGTGAAGATTAATGGTGCAGATGCTGCATTTAGGCCGGTAGATAAAAACCGCGGCAAGAATGCTAAACAAGTTAGGTGGGTTTTGCTTCTTAAAGCTCATCGTGCCGTTGGTTGTGTTACTTGGTTAGTAACTGTTCTTTGGGATTTGCTTGGTGCTATTAAGAAAAGGCTGGTTCGTAGACAGGGAGTGGCTGTTGAAAAAGGGAAATTGTTGTTTAGAATCATTAGTTTGTTTTTGTTAATTTCTTTGGCTGTTTTGGCTTTTGAGGTTGTTGCTTACTTTCAAGGTTGCCATTTTGGGAATACCAATTTGCACATTCCTCATACTTCTGATTTCCAAAGGTGGTTCCATATGGTTTATGTTGCTTGGTTGACGTTTCGTGCTGACTACATTGCTCCACCAATTCAGGCACTTTCGAAGTTTTGTATTGTTTTGTTTCTTATCCAATCCGTGGACCGTATGCTTCTTTGTTTCGGGTGGTTTTGGATCAAATTCAAGAAGGTTAAGCCAATGATCAATGGGGATCCATTCAAGGTTGATGATGTTGAAGGATCTGTATGCATTTATCCAATGGTTTTGGTTCAAATTCCAATGTGCAATGAGAAAGAGGTGTATGAGCAGTCAATTTCTGCAGTGTGTCAAATGGATTGGCCAAGGGATCGTTTACTGATTCAAGTACTTGACGATTCTGATGATGAGAGCATACAGTGGTTAATTAAGGCTGAGGTCTCTAAGTGGAACCAAAAGGGAATCAACATAATCTATCGGCATCGCTTGGTCAGGACTGGATATAAAGCTGGAAATCTTAATTCTGCAATGAACTGTGACTACGTGAAAGACTATGAATTTGTTGCAATATTTGATGCAGACTTTCAACCAAACCGTGATTTTCTTAAGCAAACTGTGCCACATTTCAAGGATAATCCTGAACTAGGTTTGGTCCAAGCTAGATGGTCTTTTGTGAACAAGGATGAGAATTTGTTGACTCGTCTCCAAAACATAAATTTATGCTTCCATTTTGAAGTAGAACAGCAGGTCAATGGGGTGTTTCTTAATTTCTTTGGTTTCAATGGAACTGCTGGTGTTTGGAGAATCAAAGCCCTAGAAGAATCTGGGGGCTGGCTGGAGAGAACAACTGTAGAAGATATGGACATTGCTGTCCGAGCTCACCTCAGTGGTTGGAAATTTATCTTCCTAAATGATGTCAAGGTACTTTGTGAAGTTCCTGAGTCATATGAAGCTTACAGGAAGCAGCAACACCGCTGGCATTCAGGGCCTATGCAACTTTTTAGGCTGTGTCTTCCAGCAATTTTGAAATCTGAGGTGTCACCGTGGAAGAAAGCAAACTTAATTCTGCTATTTTTTCTGCTAAGGAAACTAATCCTTCCCTTTTACTCCTTCACCTTATTTTGCATAATCCTTCCTTTAACCATGTTTGTTCCAGAGGCAGAACTTCCTTTATGGTTGATCTGCTATGTTCCTGTATGCATGTCAATCCTTAACATTCTTCCTGCACCTAAATCTTTTCCTTTTATTGTTCCTTACCTCCTTTTTGAAAACACCATGTCTGTGACTAAATTTAATGCAATGGTATCTGGACTCTTCCAATTGGGAAGCTCTTATGAATGGATTGTCACAAAGAAAGCTGGAAGGTCATCAGAATCTGACTTACTTGCTGCTGCAGAAAGAGAAACCGAGTCAATAGAACAACAAAAGATTCACAGAGGAGCTTCTGACAGTGGACTTGTTGAGTTACAGCAATTGAAGGAACTCAAAGAAGCAGTTCCAGAACCTGTTAAGAAAGCTAACAAGATATATAAAAAAGAGCTGACTTTGGCATTCCTTCTTCTCACAGCTTGTGTCAGAAGTCTGTTATCTGCACAAGGAGTTCATTTCTACTTTCTGTTTTTCCAAGGGATGACATTCTTCCTTGTAGGCCTTGATTTGATTGGAGAGCAGATGAGCTAG
Protein sequence

>Msa0477190

MAPRLDFSSWWMKDSQKGTPVVVKMEDPNFSVVKINGADAAFRPVDKNRGKNAKQVRWVLLLKAHRAVGCVTWLVTVLWDLLGAIKKRLVRRQGVAVEKGKLLFRIISLFLLISLAVLAFEVVAYFQGCHFGNTNLHIPHTSDFQRWFHMVYVAWLTFRADYIAPPIQALSKFCIVLFLIQSVDRMLLCFGWFWIKFKKVKPMINGDPFKVDDVEGSVCIYPMVLVQIPMCNEKEVYEQSISAVCQMDWPRDRLLIQVLDDSDDESIQWLIKAEVSKWNQKGINIIYRHRLVRTGYKAGNLNSAMNCDYVKDYEFVAIFDADFQPNRDFLKQTVPHFKDNPELGLVQARWSFVNKDENLLTRLQNINLCFHFEVEQQVNGVFLNFFGFNGTAGVWRIKALEESGGWLERTTVEDMDIAVRAHLSGWKFIFLNDVKVLCEVPESYEAYRKQQHRWHSGPMQLFRLCLPAILKSEVSPWKKANLILLFFLLRKLILPFYSFTLFCIILPLTMFVPEAELPLWLICYVPVCMSILNILPAPKSFPFIVPYLLFENTMSVTKFNAMVSGLFQLGSSYEWIVTKKAGRSSESDLLAAAERETESIEQQKIHRGASDSGLVELQQLKELKEAVPEPVKKANKIYKKELTLAFLLLTACVRSLLSAQGVHFYFLFFQGMTFFLVGLDLIGEQMS*