AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0433470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0433470 MtrunA17_Chr3g0139081 99.127 687 6 0 1 687 1 687 0.0 1403
Msa0433470 MtrunA17_Chr4g0000581 74.253 703 161 7 1 687 1 699 0.0 1043
Msa0433470 MtrunA17_Chr3g0135191 65.211 710 212 10 1 687 1 698 0.0 900
Msa0433470 MtrunA17_Chr6g0451381 66.865 673 206 6 19 687 7 666 0.0 897
Msa0433470 MtrunA17_Chr8g0339101 60.790 709 245 8 2 687 4 702 0.0 840
Msa0433470 MtrunA17_Chr1g0179381 42.481 532 269 5 155 683 19 516 9.24e-145 432
Msa0433470 MtrunA17_Chr1g0179441 42.105 532 272 5 155 683 19 517 3.23e-144 431
Msa0433470 MtrunA17_Chr1g0179421 41.541 532 275 4 155 683 19 517 2.46e-143 428
Msa0433470 MtrunA17_Chr7g0265711 41.917 532 273 5 155 683 28 526 4.32e-142 426
Msa0433470 MtrunA17_Chr1g0198111 45.188 478 229 4 207 683 79 524 1.90e-141 424
Msa0433470 MtrunA17_Chr4g0027221 41.155 537 283 7 155 683 28 539 9.40e-137 412
Msa0433470 MtrunA17_Chr7g0264621 39.332 539 280 5 154 683 24 524 3.68e-136 410
Msa0433470 MtrunA17_Chr1g0179391 49.527 317 159 1 267 582 1 317 2.35e-109 334
Msa0433470 MtrunA17_Chr4g0064791 72.535 142 33 1 189 324 59 200 5.62e-63 208
Msa0433470 MtrunA17_Chr4g0037831 80.769 104 20 0 42 145 7 110 7.41e-51 172
Msa0433470 MtrunA17_Chr4g0060331 77.778 90 20 0 42 131 7 96 4.88e-39 139
Msa0433470 MtrunA17_Chr1g0179401 41.379 116 65 2 155 268 19 133 6.92e-19 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0433470 AT4G31590.1 79.654 693 134 5 1 687 1 692 0.0 1105
Msa0433470 AT2G24630.1 76.734 692 154 5 1 687 1 690 0.0 1085
Msa0433470 AT2G24630.2 76.734 692 154 5 1 687 1 690 0.0 1085
Msa0433470 AT3G28180.1 66.618 689 187 8 21 687 6 673 0.0 915
Msa0433470 AT4G07960.1 64.316 709 219 9 1 686 1 698 0.0 882
Msa0433470 AT4G07960.2 64.316 709 219 9 1 686 1 698 0.0 882
Msa0433470 AT3G07330.1 61.842 684 250 5 6 687 8 682 0.0 857
Msa0433470 AT3G07330.2 61.842 684 250 5 6 687 8 682 0.0 857
Msa0433470 AT5G03760.1 42.830 530 263 9 159 683 33 527 1.54e-141 424
Msa0433470 AT5G22740.1 41.353 532 278 5 155 683 29 529 1.69e-140 422
Msa0433470 AT1G23480.1 41.264 538 278 6 150 683 47 550 4.79e-139 419
Msa0433470 AT1G23480.2 41.264 538 278 6 150 683 47 550 4.79e-139 419
Msa0433470 AT1G23480.3 44.050 479 233 4 207 683 33 478 6.42e-137 411
Msa0433470 AT2G35650.1 46.697 439 230 3 155 590 51 488 1.37e-135 410
Msa0433470 AT4G13410.1 43.506 462 247 3 155 608 43 498 1.50e-134 407
Msa0433470 AT2G35650.4 50.526 380 187 1 212 590 37 416 5.24e-134 403
Msa0433470 AT2G35650.2 50.526 380 187 1 212 590 37 416 5.24e-134 403
Msa0433470 AT5G16190.3 40.490 531 281 4 155 683 6 503 1.36e-133 403
Msa0433470 AT1G24070.1 38.941 529 286 5 158 683 57 551 4.29e-133 403
Msa0433470 AT5G16190.1 42.495 473 238 3 212 683 3 442 1.30e-130 393
Msa0433470 AT5G16190.2 45.372 443 240 2 155 595 6 448 1.36e-130 394
Msa0433470 AT3G56000.1 40.449 534 273 7 159 683 26 523 1.75e-130 396
Msa0433470 AT5G16190.4 38.121 564 281 5 155 683 6 536 2.73e-126 385
Msa0433470 AT4G16590.1 37.222 540 267 8 147 683 49 519 3.50e-114 353
Msa0433470 AT1G23480.4 42.344 418 206 4 268 683 21 405 1.43e-111 343
Msa0433470 AT2G35650.3 48.447 322 165 1 270 590 9 330 1.50e-106 329
Msa0433470 AT4G13410.3 45.833 336 181 1 274 608 2 337 5.48e-106 327
Msa0433470 AT1G24070.2 48.276 319 162 2 158 474 57 374 7.57e-102 317
Msa0433470 AT4G13410.2 47.706 327 158 2 155 474 43 363 1.85e-100 313

Find 188 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCAGTGTCACTAATTT+TGG 0.054016 3_2:-85967382 None:intergenic
TACTTTCAAGGTTGGCATTT+TGG 0.120896 3_2:-85966647 Msa0433470:CDS
AATCTTCACAACTGAGAAAT+TGG 0.125987 3_2:+85966937 None:intergenic
GTTAATTTCTTTGGCTGTTT+TGG 0.142194 3_2:-85966687 Msa0433470:CDS
ACCGTATGCTTCTTTGTTTC+GGG 0.170225 3_2:-85966469 Msa0433470:CDS
CAGAGGCAGAACTTCCTTTA+TGG 0.175113 3_2:-85964560 Msa0433470:CDS
TTCTCAGTTGTGAAGATTAA+TGG 0.191808 3_2:-85966932 Msa0433470:CDS
CAGCAACACCGCTGGCATTC+AGG 0.192229 3_2:-85965063 Msa0433470:CDS
CTTGGTTAGTAACTGTTCTT+TGG 0.224084 3_2:-85966805 Msa0433470:CDS
GGGGTGTTTCTTAATTTCTT+TGG 0.229657 3_2:-85965438 Msa0433470:CDS
TTAGCTTTCTTAACAGGTTC+TGG 0.235289 3_2:+85964216 None:intergenic
AAATAAGGTGAAGGAGTAAA+AGG 0.246621 3_2:+85964614 None:intergenic
TTCTTTGTTTCGGGTGGTTT+TGG 0.256673 3_2:-85966460 Msa0433470:CDS
GGAAGCATAAATTTATGTTT+TGG 0.261801 3_2:+85965487 None:intergenic
AAATTTAATGCAATGGTATC+TGG 0.270707 3_2:-85964426 Msa0433470:CDS
CCTGCTGTTCTACTTCAAAA+TGG 0.273610 3_2:+85965466 None:intergenic
AGATTCGTAGTTGATGTTAT+TGG 0.273969 3_2:+85967214 None:intergenic
CAGGGAGTGGCTGTTGAAAA+AGG 0.279089 3_2:-85966743 Msa0433470:CDS
ATGCATTTATCCAATGGTTT+TGG 0.280954 3_2:-85966366 Msa0433470:CDS
TTTGTTCTTTGGTTGTATTT+GGG 0.283510 3_2:-85963610 Msa0433470:three_prime_UTR
CTTAATTTCTTTGGTTTCAA+TGG 0.284700 3_2:-85965429 Msa0433470:CDS
GACCGTATGCTTCTTTGTTT+CGG 0.285549 3_2:-85966470 Msa0433470:CDS
TCCAGTGACCAGTGCCTATA+TGG 0.291143 3_2:+85963702 None:intergenic
TGATCACCTTGACATCATTT+AGG 0.295314 3_2:+85965286 None:intergenic
AGGAGGTAAGGAACAATAAA+AGG 0.298765 3_2:+85964474 None:intergenic
ATCTTGTTAGCTTTCTTAAC+AGG 0.305652 3_2:+85964210 None:intergenic
GAGGTTGTTGCTTACTTTCA+AGG 0.311089 3_2:-85966659 Msa0433470:CDS
ACAGTTTGCTTAAGAAAATC+AGG 0.311338 3_2:+85965687 None:intergenic
ACAATAAAAGGAAAAGATTT+AGG 0.311629 3_2:+85964486 None:intergenic
ACCTCAGATTTCAAAATTGC+TGG 0.313915 3_2:+85965012 None:intergenic
CTGAGTCATATGAAGCTTAC+AGG 0.314147 3_2:-85965089 Msa0433470:CDS
AGATTATGTTGATTCCCTTT+TGG 0.319352 3_2:+85965829 None:intergenic
CTTGTAGGCCTTGATTTGAT+TGG 0.321359 3_2:-85964072 Msa0433470:CDS
AACACCAGTGGTGGTGAAAA+TGG 0.333264 3_2:-85966966 Msa0433470:CDS
AGGAAGGATTATGCAAAATA+AGG 0.334621 3_2:+85964599 None:intergenic
ATGAACTCTGCTCAGACATT+TGG 0.344263 3_2:+85963551 None:intergenic
GTGCCGTTGGTTGTGTTACT+TGG 0.350606 3_2:-85966823 Msa0433470:CDS
ATTTACAATGGCTCCAAGAT+TGG 0.353231 3_2:-85967026 Msa0433470:exon
CATGAGTTAAGTAAATAAAA+TGG 0.353932 3_2:-85963398 Msa0433470:three_prime_UTR
TCTGGAACAAACATGGTTAA+AGG 0.354104 3_2:+85964579 None:intergenic
CTACATTGCTCCACCAATTC+AGG 0.355815 3_2:-85966537 Msa0433470:CDS
AAACCATATGGAACCACCTT+TGG 0.363318 3_2:+85966588 None:intergenic
GCAAACTGTGCCACATTTCA+AGG 0.370258 3_2:-85965673 Msa0433470:intron
TTTGTTGCTAATTGTATTGC+TGG 0.372081 3_2:+85963867 None:intergenic
TCCATATGGTTTATGTTGCT+TGG 0.374551 3_2:-85966577 Msa0433470:CDS
GAGCATACAGTGGTTAATTA+AGG 0.378443 3_2:-85965871 Msa0433470:CDS
TTGGTTAGTAACTGTTCTTT+GGG 0.379476 3_2:-85966804 Msa0433470:CDS
ATCAAAGCCCTAGAAGAATC+TGG 0.380417 3_2:-85965387 Msa0433470:CDS
ATCTTCACAACTGAGAAATT+GGG 0.381353 3_2:+85966938 None:intergenic
TCCATATAGGCACTGGTCAC+TGG 0.387795 3_2:-85963703 Msa0433470:three_prime_UTR
TGCTTAAGAAAATCAGGGTT+TGG 0.389882 3_2:+85965693 None:intergenic
AGCAACACCGCTGGCATTCA+GGG 0.390128 3_2:-85965062 Msa0433470:CDS
ATAACAGAGAAACCTTGTTT+TGG 0.396626 3_2:+85963773 None:intergenic
GGTATCTGGACTCTTCCAAT+TGG 0.403642 3_2:-85964412 Msa0433470:CDS
GGGATGACATTCTTCCTTGT+AGG 0.403705 3_2:-85964087 Msa0433470:CDS
AATTGGGAAGCTCTTATGAA+TGG 0.404164 3_2:-85964395 Msa0433470:CDS
AATGGATCCCCATTGATCAT+TGG 0.406112 3_2:+85966416 None:intergenic
GCTTGGACCAAACCTAGTTC+AGG 0.414765 3_2:+85965555 None:intergenic
GAGAACAACTGTAGAAGATA+TGG 0.416414 3_2:-85965352 Msa0433470:CDS
TTTCCAAAGGTGGTTCCATA+TGG 0.418411 3_2:-85966591 Msa0433470:CDS
TTTGGTTTCAATGGAACTGC+TGG 0.420392 3_2:-85965420 Msa0433470:CDS
TGGAACGTGAAACACACTAT+TGG 0.421234 3_2:+85963990 None:intergenic
CAACACGGGTTCAGCATCCT+TGG 0.430491 3_2:+85963970 None:intergenic
GCAAGAATGCTAAACAAGTT+AGG 0.432413 3_2:-85966871 Msa0433470:CDS
AGTATGAGGAATGTGCAAAT+TGG 0.435051 3_2:+85966619 None:intergenic
AGGTTAAGCCAATGATCAAT+GGG 0.437598 3_2:-85966424 Msa0433470:CDS
GAAGTAGAACAGCAGGTCAA+TGG 0.438411 3_2:-85965459 Msa0433470:CDS
GAAAAGGCTGGTTCGTAGAC+AGG 0.438680 3_2:-85966762 Msa0433470:CDS
AACTTCGAAAGTGCCTGAAT+TGG 0.444764 3_2:+85966524 None:intergenic
AATTTCTGCAGTGTGTCAAA+TGG 0.446405 3_2:-85965946 Msa0433470:CDS
TTCGAAAGTGCCTGAATTGG+TGG 0.450121 3_2:+85966527 None:intergenic
TCAATGGAACTGCTGGTGTT+TGG 0.451289 3_2:-85965413 Msa0433470:CDS
ACCAAGCAACATAAACCATA+TGG 0.452313 3_2:+85966576 None:intergenic
AAGTAGAACAGCAGGTCAAT+GGG 0.453067 3_2:-85965458 Msa0433470:CDS
GGATAATCCTGAACTAGGTT+TGG 0.455019 3_2:-85965562 Msa0433470:CDS
ATTGGAAAAGGCACATTATG+AGG 0.455854 3_2:+85967278 None:intergenic
ACATAGCAGATCAACCATAA+AGG 0.458153 3_2:+85964546 None:intergenic
GAATAAGACACAAACTTTCA+AGG 0.461463 3_2:+85967309 None:intergenic
AAAGGAAAAGATTTAGGTGC+AGG 0.463485 3_2:+85964492 None:intergenic
TGTTGAGTTACAGCAATTGA+AGG 0.464132 3_2:-85964256 Msa0433470:CDS
TGCTTGGTGCTATTAAGAAA+AGG 0.466972 3_2:-85966778 Msa0433470:CDS
GGAATTTGAACCAAAACCAT+TGG 0.468674 3_2:+85966356 None:intergenic
TCAACATCATCAACCTTGAA+TGG 0.468739 3_2:+85966398 None:intergenic
AAGACACCTAACATTGGAAA+AGG 0.469988 3_2:+85967266 None:intergenic
GGTCTCTAAGTGGAACCAAA+AGG 0.473118 3_2:-85965844 Msa0433470:CDS
GACATTTGGAATGGTGAGAT+AGG 0.474946 3_2:+85963565 None:intergenic
ATCTGTATGCATTTATCCAA+TGG 0.475291 3_2:-85966372 Msa0433470:CDS
AAGGTTAAGCCAATGATCAA+TGG 0.477569 3_2:-85966425 Msa0433470:CDS
CGGCATCGCTTGGTCAGGAC+TGG 0.477703 3_2:-85965804 Msa0433470:CDS
ATTCATAAGAGCTTCCCAAT+TGG 0.477893 3_2:+85964397 None:intergenic
TCTATCGGCATCGCTTGGTC+AGG 0.480004 3_2:-85965809 Msa0433470:CDS
TTACAGGATAATCCTGAACT+AGG 0.481264 3_2:-85965567 Msa0433470:intron
TCCAATGTGCAATGAGAAAG+AGG 0.482610 3_2:-85966336 Msa0433470:intron
GTCAGGACTGGATATAAAGC+TGG 0.486529 3_2:-85965792 Msa0433470:CDS
TCATCACAAGTATTCCATAT+AGG 0.488036 3_2:-85963716 Msa0433470:three_prime_UTR
ACTGTTCTTTGGGATTTGCT+TGG 0.488047 3_2:-85966794 Msa0433470:CDS
CGTGTCAGCGCACAATTGAA+TGG 0.488875 3_2:+85963928 None:intergenic
ATTAAGTTTGCTTTCTTCCA+CGG 0.493411 3_2:+85964672 None:intergenic
GAAGCATACGGTCCACGGAT+TGG 0.496178 3_2:+85966480 None:intergenic
AATAAGGTGAAGGAGTAAAA+GGG 0.501255 3_2:+85964615 None:intergenic
GATCAATGGGGATCCATTCA+AGG 0.501987 3_2:-85966411 Msa0433470:CDS
TGACACAAGCTGTGAGAAGA+AGG 0.502725 3_2:+85964160 None:intergenic
TGGATTGTCACAAAGAAAGC+TGG 0.503165 3_2:-85964375 Msa0433470:CDS
ATATTTACAGTGATCAATCA+TGG 0.503419 3_2:+85967339 None:intergenic
TCCGAGCTCACCTCAATGGT+TGG 0.504060 3_2:-85965320 Msa0433470:CDS
CAAGGAAGAATGTCATCCCT+TGG 0.504529 3_2:+85964091 None:intergenic
ATGGTGCAGATGCTGCATTT+AGG 0.506624 3_2:-85966913 Msa0433470:CDS
GTAAATAAAATGGTGTCGGC+AGG 0.506989 3_2:-85963388 None:intergenic
TCAAAGCCCTAGAAGAATCT+GGG 0.507079 3_2:-85965386 Msa0433470:CDS
TCCAACCATTGAGGTGAGCT+CGG 0.507425 3_2:+85965319 None:intergenic
AATGGGTCAAACAGAAATGA+AGG 0.507855 3_2:+85963482 None:intergenic
TTGTTGCTTACTTTCAAGGT+TGG 0.508405 3_2:-85966655 Msa0433470:CDS
CTCATACTTCTGATTTCCAA+AGG 0.508694 3_2:-85966604 Msa0433470:CDS
GTATCTGGACTCTTCCAATT+GGG 0.517060 3_2:-85964411 Msa0433470:CDS
TTCAAGGTTGATGATGTTGA+AGG 0.517442 3_2:-85966395 Msa0433470:CDS
CTTAAAGCTCATCGTGCCGT+TGG 0.518642 3_2:-85966836 Msa0433470:CDS
CATAAAGGAAGTTCTGCCTC+TGG 0.523961 3_2:+85964561 None:intergenic
ATAGTGTGTTTCACGTTCCA+AGG 0.526080 3_2:-85963987 Msa0433470:three_prime_UTR
GTCTCTAAGTGGAACCAAAA+GGG 0.527274 3_2:-85965843 Msa0433470:CDS
CTTTGGAAATCAGAAGTATG+AGG 0.527295 3_2:+85966605 None:intergenic
GCTGTCCGAGCTCACCTCAA+TGG 0.528881 3_2:-85965324 Msa0433470:CDS
CAATCAAATCAAGGCCTACA+AGG 0.531066 3_2:+85964073 None:intergenic
ACCTCTTTCTCATTGCACAT+TGG 0.537709 3_2:+85966335 None:intergenic
CATAATCTATCGGCATCGCT+TGG 0.540008 3_2:-85965814 Msa0433470:CDS
ACAAAGAAGCATACGGTCCA+CGG 0.540829 3_2:+85966475 None:intergenic
AAAAGGCTGGTTCGTAGACA+GGG 0.541112 3_2:-85966761 Msa0433470:CDS
TCTGCTCTCCAATCAAATCA+AGG 0.541124 3_2:+85964064 None:intergenic
TGGTGCTATTAAGAAAAGGC+TGG 0.543425 3_2:-85966774 Msa0433470:CDS
CTGTTCTGAGAGTTTCAGAA+AGG 0.544450 3_2:+85963824 None:intergenic
AGATAAATTTCCAACCATTG+AGG 0.544758 3_2:+85965310 None:intergenic
TTTAACCATGTTTGTTCCAG+AGG 0.547320 3_2:-85964577 Msa0433470:CDS
CTGCAGTGTGTCAAATGGAT+TGG 0.548428 3_2:-85965941 Msa0433470:CDS
TTAAGGATTGACATGCATAC+AGG 0.551931 3_2:+85964522 None:intergenic
TTTGTTTCTTATCCAATCCG+TGG 0.553534 3_2:-85966492 Msa0433470:CDS
TATCTTCCTAAATGATGTCA+AGG 0.553615 3_2:-85965292 Msa0433470:intron
TCACAAAAGACCATCTAGCT+TGG 0.556166 3_2:+85965538 None:intergenic
GAATGCTAAACAAGTTAGGT+GGG 0.557662 3_2:-85966867 Msa0433470:CDS
GCTGGTTCGTAGACAGGGAG+TGG 0.560932 3_2:-85966756 Msa0433470:CDS
TGAATCAGTAAACGATCCCT+TGG 0.561328 3_2:+85965918 None:intergenic
TAGGTGCAGGAAGAATGTTA+AGG 0.561478 3_2:+85964505 None:intergenic
AGGGAATCAACATAATCTAT+CGG 0.563806 3_2:-85965824 Msa0433470:CDS
TGTGTCAAATGGATTGGCCA+AGG 0.564789 3_2:-85965935 Msa0433470:CDS
CAAGTATTCCATATAGGCAC+TGG 0.568611 3_2:-85963710 Msa0433470:three_prime_UTR
AGAATGCTAAACAAGTTAGG+TGG 0.573310 3_2:-85966868 Msa0433470:CDS
TGCAGATGCTGCATTTAGGC+CGG 0.573889 3_2:-85966909 Msa0433470:CDS
TCTTCACAACTGAGAAATTG+GGG 0.578230 3_2:+85966939 None:intergenic
ACAGGAAGCAGCAACACCGC+TGG 0.578674 3_2:-85965071 Msa0433470:CDS
GTATGCTTCTTTGTTTCGGG+TGG 0.578929 3_2:-85966466 Msa0433470:CDS
CTGTAAGCTTCATATGACTC+AGG 0.579123 3_2:+85965090 None:intergenic
TAGGTTTGGTCCAAGCTAGA+TGG 0.582078 3_2:-85965548 Msa0433470:CDS
ACAGTGGTTAATTAAGGCTG+AGG 0.582788 3_2:-85965865 Msa0433470:CDS
GAACAAACATGGTTAAAGGA+AGG 0.583578 3_2:+85964583 None:intergenic
TTAAGTAAATAAAATGGTGT+CGG 0.583799 3_2:-85963392 Msa0433470:three_prime_UTR
CAGTTTGCTTAAGAAAATCA+GGG 0.584235 3_2:+85965688 None:intergenic
TGGATGAAGGATTCACAGAA+AGG 0.587309 3_2:-85966989 Msa0433470:CDS
CTTGTTTAGCATTCTTGCCG+CGG 0.588225 3_2:+85966876 None:intergenic
CTCTGCTCAGACATTTGGAA+TGG 0.590647 3_2:+85963556 None:intergenic
ATACTTCTGATTTCCAAAGG+TGG 0.593673 3_2:-85966601 Msa0433470:CDS
TGTGACTAAATTTAATGCAA+TGG 0.593823 3_2:-85964433 Msa0433470:CDS
TTCTGCCTCTGGAACAAACA+TGG 0.595292 3_2:+85964572 None:intergenic
GATTATGCAAAATAAGGTGA+AGG 0.598839 3_2:+85964605 None:intergenic
AGGTGAAGGAGTAAAAGGGA+AGG 0.604134 3_2:+85964619 None:intergenic
CTTCACTTAACCTTGAAATG+TGG 0.610582 3_2:+85965663 None:intergenic
CAAAGCCCTAGAAGAATCTG+GGG 0.611476 3_2:-85965385 Msa0433470:CDS
CACAGAGGAGCTTCTGACAG+TGG 0.622297 3_2:-85964282 Msa0433470:CDS
TAACCAAGTAACACAACCAA+CGG 0.623439 3_2:+85966820 None:intergenic
GTGTCAAATGGATTGGCCAA+GGG 0.623875 3_2:-85965934 Msa0433470:CDS
TACATATAGTTGATTCAACA+CGG 0.632486 3_2:+85963955 None:intergenic
ACCCGAAACAAAGAAGCATA+CGG 0.633312 3_2:+85966468 None:intergenic
TTAAGGCTGAGGTCTCTAAG+TGG 0.635565 3_2:-85965854 Msa0433470:CDS
ACAGAAAGGAACACCAGTGG+TGG 0.639233 3_2:-85966975 Msa0433470:CDS
ATTAAATTTAGTCACAGACA+TGG 0.642279 3_2:+85964440 None:intergenic
TGTGAAACTGTGATTTACAA+TGG 0.643682 3_2:-85967038 Msa0433470:exon
GACAACAAAGAGTAAAGACA+TGG 0.646506 3_2:+85967167 None:intergenic
ACTGCAACTTATAGTGTGTG+TGG 0.653445 3_2:+85963890 None:intergenic
AGAAGTCTGTTATCTGCACA+AGG 0.657709 3_2:-85964138 Msa0433470:CDS
ACATATAGTTGATTCAACAC+GGG 0.660206 3_2:+85963956 None:intergenic
GCATAGGCCCTGAATGCCAG+CGG 0.668252 3_2:+85965055 None:intergenic
TGATTAATCAGCAGAAGATG+TGG 0.669212 3_2:+85964014 None:intergenic
TTGTCACAAAGAAAGCTGGA+AGG 0.675157 3_2:-85964371 Msa0433470:CDS
ACCAGTGGTGGTGAAAATGG+AGG 0.695679 3_2:-85966963 Msa0433470:CDS
AGTAGAACAGCAGGTCAATG+GGG 0.717208 3_2:-85965457 Msa0433470:CDS
TTCACAGAAAGGAACACCAG+TGG 0.722164 3_2:-85966978 Msa0433470:CDS
GAACAACAAAAGATTCACAG+AGG 0.731952 3_2:-85964297 Msa0433470:CDS
AAAGCCCTAGAAGAATCTGG+GGG 0.740097 3_2:-85965384 Msa0433470:CDS
CTGATGATGAGAGCATACAG+TGG 0.744429 3_2:-85965881 Msa0433470:CDS
TTTAACTGCAGGTGTCACCG+TGG 0.746704 3_2:-85964689 Msa0433470:intron
GGTTAAGCCAATGATCAATG+GGG 0.761083 3_2:-85966423 Msa0433470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTTTTTGTTAATTTCTT+TGG - chr3_2:85964064-85964083 Msa0433470:CDS 15.0%
!! AAAACAAAGAAAAAACAAAT+GGG + chr3_2:85967298-85967317 None:intergenic 15.0%
!! AAAAACAAAGAAAAAACAAA+TGG + chr3_2:85967299-85967318 None:intergenic 15.0%
!! CTTAATTGTGTTTAAATTTC+AGG - chr3_2:85965633-85965652 Msa0433470:intron 20.0%
!!! AATAAAATTTAAGACCCTTT+AGG + chr3_2:85966053-85966072 None:intergenic 20.0%
!!! CTTAAATTTTATTTAACTGC+AGG - chr3_2:85966060-85966079 Msa0433470:intron 20.0%
!! ACAATAAAAGGAAAAGATTT+AGG + chr3_2:85966277-85966296 None:intergenic 20.0%
!!! TTATTTTAAGTACCAAAACA+AGG - chr3_2:85966975-85966994 Msa0433470:CDS 20.0%
!!! GTTTTCTTTATTTTGTTCTT+TGG - chr3_2:85967139-85967158 Msa0433470:five_prime_UTR 20.0%
!!! TTTGTTTTTTTGTTGTTACT+TGG - chr3_2:85967310-85967329 Msa0433470:five_prime_UTR 20.0%
!! CATGAGTTAAGTAAATAAAA+TGG - chr3_2:85967362-85967381 Msa0433470:five_prime_UTR 20.0%
!! TTAAGTAAATAAAATGGTGT+CGG - chr3_2:85967368-85967387 Msa0433470:five_prime_UTR 20.0%
! ATATTTACAGTGATCAATCA+TGG + chr3_2:85963424-85963443 None:intergenic 25.0%
! ACTATTACATTGAAACTTAC+TGG + chr3_2:85964513-85964532 None:intergenic 25.0%
! AGATTGCAGAAAATTCTTAA+GGG + chr3_2:85964642-85964661 None:intergenic 25.0%
! TCAATGACATTGACAAAATA+TGG - chr3_2:85964735-85964754 Msa0433470:intron 25.0%
!!! GGAAGCATAAATTTATGTTT+TGG + chr3_2:85965276-85965295 None:intergenic 25.0%
!! CTTAATTTCTTTGGTTTCAA+TGG - chr3_2:85965331-85965350 Msa0433470:CDS 25.0%
! TATTATGCACTATGAAAGAA+AGG + chr3_2:85965537-85965556 None:intergenic 25.0%
! AAATTTAAGAGAGTTCTTCA+GGG + chr3_2:85965613-85965632 None:intergenic 25.0%
!!! TATAAAGTGTTGTCAAATAG+CGG - chr3_2:85965908-85965927 Msa0433470:CDS 25.0%
! TACTGAGTATTTGCAATTAT+TGG - chr3_2:85966012-85966031 Msa0433470:intron 25.0%
! TTATATTATGTACTGCCTAA+AGG - chr3_2:85966035-85966054 Msa0433470:intron 25.0%
! TATATTATGTACTGCCTAAA+GGG - chr3_2:85966036-85966055 Msa0433470:intron 25.0%
! ATTAAATTTAGTCACAGACA+TGG + chr3_2:85966323-85966342 None:intergenic 25.0%
! TGTGACTAAATTTAATGCAA+TGG - chr3_2:85966327-85966346 Msa0433470:intron 25.0%
! AAATTTAATGCAATGGTATC+TGG - chr3_2:85966334-85966353 Msa0433470:intron 25.0%
!! ATATAAAAAAGAGCTGACTT+TGG - chr3_2:85966573-85966592 Msa0433470:CDS 25.0%
!! TACATATAGTTGATTCAACA+CGG + chr3_2:85966808-85966827 None:intergenic 25.0%
! GAATGAATGAAAAATCGATA+GGG + chr3_2:85967017-85967036 None:intergenic 25.0%
! TGAATGAATGAAAAATCGAT+AGG + chr3_2:85967018-85967037 None:intergenic 25.0%
!! TTTTGTTCTTTGGTTGTATT+TGG - chr3_2:85967149-85967168 Msa0433470:five_prime_UTR 25.0%
! TTTGTTCTTTGGTTGTATTT+GGG - chr3_2:85967150-85967169 Msa0433470:five_prime_UTR 25.0%
GAATAAGACACAAACTTTCA+AGG + chr3_2:85963454-85963473 None:intergenic 30.0%
!! TTTTTGAAGACACCTAACAT+TGG + chr3_2:85963503-85963522 None:intergenic 30.0%
! AGATTCGTAGTTGATGTTAT+TGG + chr3_2:85963549-85963568 None:intergenic 30.0%
TGTGAAACTGTGATTTACAA+TGG - chr3_2:85963722-85963741 Msa0433470:three_prime_UTR 30.0%
CAACTTGAAAAATCCAATCT+TGG + chr3_2:85963750-85963769 None:intergenic 30.0%
!!! CAAGATTGGATTTTTCAAGT+TGG - chr3_2:85963748-85963767 Msa0433470:three_prime_UTR 30.0%
ATCTTCACAACTGAGAAATT+GGG + chr3_2:85963825-85963844 None:intergenic 30.0%
AATCTTCACAACTGAGAAAT+TGG + chr3_2:85963826-85963845 None:intergenic 30.0%
TTCTCAGTTGTGAAGATTAA+TGG - chr3_2:85963828-85963847 Msa0433470:three_prime_UTR 30.0%
TTGGTTAGTAACTGTTCTTT+GGG - chr3_2:85963956-85963975 Msa0433470:three_prime_UTR 30.0%
! GTTAATTTCTTTGGCTGTTT+TGG - chr3_2:85964073-85964092 Msa0433470:CDS 30.0%
!! GTTTTGGATCAAATTCAAGA+AGG - chr3_2:85964316-85964335 Msa0433470:CDS 30.0%
ATCTGTATGCATTTATCCAA+TGG - chr3_2:85964388-85964407 Msa0433470:CDS 30.0%
! ATGCATTTATCCAATGGTTT+TGG - chr3_2:85964394-85964413 Msa0433470:CDS 30.0%
TAAAAACCTCATAGCAAAAC+TGG + chr3_2:85964472-85964491 None:intergenic 30.0%
GAGATTGCAGAAAATTCTTA+AGG + chr3_2:85964643-85964662 None:intergenic 30.0%
TAAAACAACACAGCATCAAA+TGG + chr3_2:85964691-85964710 None:intergenic 30.0%
!!! AGATTATGTTGATTCCCTTT+TGG + chr3_2:85964934-85964953 None:intergenic 30.0%
AGGGAATCAACATAATCTAT+CGG - chr3_2:85964936-85964955 Msa0433470:intron 30.0%
! CAGTTTGCTTAAGAAAATCA+GGG + chr3_2:85965075-85965094 None:intergenic 30.0%
! ACAGTTTGCTTAAGAAAATC+AGG + chr3_2:85965076-85965095 None:intergenic 30.0%
!! TGCAAATGACTTGTTATTAC+AGG - chr3_2:85965177-85965196 Msa0433470:intron 30.0%
AGATAAATTTCCAACCATTG+AGG + chr3_2:85965453-85965472 None:intergenic 30.0%
TATCTTCCTAAATGATGTCA+AGG - chr3_2:85965468-85965487 Msa0433470:CDS 30.0%
GAAATTTAAGAGAGTTCTTC+AGG + chr3_2:85965614-85965633 None:intergenic 30.0%
! ATTAAGTTTGCTTTCTTCCA+CGG + chr3_2:85966091-85966110 None:intergenic 30.0%
!!! TTCTGCTATTTTTTCTGCTA+AGG - chr3_2:85966110-85966129 Msa0433470:intron 30.0%
AATAAGGTGAAGGAGTAAAA+GGG + chr3_2:85966148-85966167 None:intergenic 30.0%
AAATAAGGTGAAGGAGTAAA+AGG + chr3_2:85966149-85966168 None:intergenic 30.0%
GATTATGCAAAATAAGGTGA+AGG + chr3_2:85966158-85966177 None:intergenic 30.0%
AGGAAGGATTATGCAAAATA+AGG + chr3_2:85966164-85966183 None:intergenic 30.0%
!!! CTTTTGTTGTTCTATTGACT+CGG + chr3_2:85966455-85966474 None:intergenic 30.0%
!! ATCTTGTTAGCTTTCTTAAC+AGG + chr3_2:85966553-85966572 None:intergenic 30.0%
!! TTCTACTTTCTGTTTTTCCA+AGG - chr3_2:85966652-85966671 Msa0433470:CDS 30.0%
!! TCTACTTTCTGTTTTTCCAA+GGG - chr3_2:85966653-85966672 Msa0433470:CDS 30.0%
! ACATATAGTTGATTCAACAC+GGG + chr3_2:85966807-85966826 None:intergenic 30.0%
TTTGTTGCTAATTGTATTGC+TGG + chr3_2:85966896-85966915 None:intergenic 30.0%
!! ATAACAGAGAAACCTTGTTT+TGG + chr3_2:85966990-85967009 None:intergenic 30.0%
TCATCACAAGTATTCCATAT+AGG - chr3_2:85967044-85967063 Msa0433470:five_prime_UTR 30.0%
! ATTGGAAAAGGCACATTATG+AGG + chr3_2:85963485-85963504 None:intergenic 35.0%
! AATGTGCCTTTTCCAATGTT+AGG - chr3_2:85963488-85963507 Msa0433470:three_prime_UTR 35.0%
AAGACACCTAACATTGGAAA+AGG + chr3_2:85963497-85963516 None:intergenic 35.0%
GACAACAAAGAGTAAAGACA+TGG + chr3_2:85963596-85963615 None:intergenic 35.0%
ATTTACAATGGCTCCAAGAT+TGG - chr3_2:85963734-85963753 Msa0433470:three_prime_UTR 35.0%
!!! GATTGGATTTTTCAAGTTGG+TGG - chr3_2:85963751-85963770 Msa0433470:three_prime_UTR 35.0%
!!! TTTTTCAAGTTGGTGGATGA+AGG - chr3_2:85963758-85963777 Msa0433470:three_prime_UTR 35.0%
TCTTCACAACTGAGAAATTG+GGG + chr3_2:85963824-85963843 None:intergenic 35.0%
GCAAGAATGCTAAACAAGTT+AGG - chr3_2:85963889-85963908 Msa0433470:three_prime_UTR 35.0%
AGAATGCTAAACAAGTTAGG+TGG - chr3_2:85963892-85963911 Msa0433470:three_prime_UTR 35.0%
GAATGCTAAACAAGTTAGGT+GGG - chr3_2:85963893-85963912 Msa0433470:three_prime_UTR 35.0%
TAACCAAGTAACACAACCAA+CGG + chr3_2:85963943-85963962 None:intergenic 35.0%
CTTGGTTAGTAACTGTTCTT+TGG - chr3_2:85963955-85963974 Msa0433470:three_prime_UTR 35.0%
!! TGCTTGGTGCTATTAAGAAA+AGG - chr3_2:85963982-85964001 Msa0433470:three_prime_UTR 35.0%
! TTGTTGCTTACTTTCAAGGT+TGG - chr3_2:85964105-85964124 Msa0433470:CDS 35.0%
! TACTTTCAAGGTTGGCATTT+TGG - chr3_2:85964113-85964132 Msa0433470:CDS 35.0%
! ACTTTCAAGGTTGGCATTTT+GGG - chr3_2:85964114-85964133 Msa0433470:CDS 35.0%
AGTATGAGGAATGTGCAAAT+TGG + chr3_2:85964144-85964163 None:intergenic 35.0%
! CTTTGGAAATCAGAAGTATG+AGG + chr3_2:85964158-85964177 None:intergenic 35.0%
CTCATACTTCTGATTTCCAA+AGG - chr3_2:85964156-85964175 Msa0433470:CDS 35.0%
ATACTTCTGATTTCCAAAGG+TGG - chr3_2:85964159-85964178 Msa0433470:CDS 35.0%
TCCATATGGTTTATGTTGCT+TGG - chr3_2:85964183-85964202 Msa0433470:CDS 35.0%
ACCAAGCAACATAAACCATA+TGG + chr3_2:85964187-85964206 None:intergenic 35.0%
! TTTGTTTCTTATCCAATCCG+TGG - chr3_2:85964268-85964287 Msa0433470:CDS 35.0%
AAGGTTAAGCCAATGATCAA+TGG - chr3_2:85964335-85964354 Msa0433470:CDS 35.0%
AGGTTAAGCCAATGATCAAT+GGG - chr3_2:85964336-85964355 Msa0433470:CDS 35.0%
TCAACATCATCAACCTTGAA+TGG + chr3_2:85964365-85964384 None:intergenic 35.0%
!! TTCAAGGTTGATGATGTTGA+AGG - chr3_2:85964365-85964384 Msa0433470:CDS 35.0%
GGAATTTGAACCAAAACCAT+TGG + chr3_2:85964407-85964426 None:intergenic 35.0%
AAAACTGGAATGCAGAAGTT+CGG + chr3_2:85964457-85964476 None:intergenic 35.0%
! GCATTTGTGTTTCTGAGTTT+AGG + chr3_2:85964665-85964684 None:intergenic 35.0%
! TGCACAAATTGACTTTATGC+AGG - chr3_2:85964778-85964797 Msa0433470:intron 35.0%
AATTTCTGCAGTGTGTCAAA+TGG - chr3_2:85964814-85964833 Msa0433470:intron 35.0%
GAGCATACAGTGGTTAATTA+AGG - chr3_2:85964889-85964908 Msa0433470:intron 35.0%
TGCTTAAGAAAATCAGGGTT+TGG + chr3_2:85965070-85965089 None:intergenic 35.0%
CTTCACTTAACCTTGAAATG+TGG + chr3_2:85965100-85965119 None:intergenic 35.0%
TTACAGGATAATCCTGAACT+AGG - chr3_2:85965193-85965212 Msa0433470:intron 35.0%
! TAGATGGTCTTTTGTGAACA+AGG - chr3_2:85965228-85965247 Msa0433470:intron 35.0%
! GGGGTGTTTCTTAATTTCTT+TGG - chr3_2:85965322-85965341 Msa0433470:CDS 35.0%
GAGAACAACTGTAGAAGATA+TGG - chr3_2:85965408-85965427 Msa0433470:CDS 35.0%
TGATCACCTTGACATCATTT+AGG + chr3_2:85965477-85965496 None:intergenic 35.0%
ACAGAAGTTCGATTTCATGA+AGG + chr3_2:85965575-85965594 None:intergenic 35.0%
!!! TCCAGCAATTTTGAAATCTG+AGG - chr3_2:85965747-85965766 Msa0433470:CDS 35.0%
ACCTCAGATTTCAAAATTGC+TGG + chr3_2:85965751-85965770 None:intergenic 35.0%
TTGCAGATCGCAAAAAATAG+TGG + chr3_2:85965979-85965998 None:intergenic 35.0%
GAACAAACATGGTTAAAGGA+AGG + chr3_2:85966180-85966199 None:intergenic 35.0%
! TCTGGAACAAACATGGTTAA+AGG + chr3_2:85966184-85966203 None:intergenic 35.0%
TTTAACCATGTTTGTTCCAG+AGG - chr3_2:85966183-85966202 Msa0433470:intron 35.0%
ACATAGCAGATCAACCATAA+AGG + chr3_2:85966217-85966236 None:intergenic 35.0%
TTAAGGATTGACATGCATAC+AGG + chr3_2:85966241-85966260 None:intergenic 35.0%
AAAGGAAAAGATTTAGGTGC+AGG + chr3_2:85966271-85966290 None:intergenic 35.0%
AGGAGGTAAGGAACAATAAA+AGG + chr3_2:85966289-85966308 None:intergenic 35.0%
! GTGTTTTCAAAAAGGAGGTA+AGG + chr3_2:85966301-85966320 None:intergenic 35.0%
! ACATGGTGTTTTCAAAAAGG+AGG + chr3_2:85966306-85966325 None:intergenic 35.0%
! CAGACATGGTGTTTTCAAAA+AGG + chr3_2:85966309-85966328 None:intergenic 35.0%
ATTCATAAGAGCTTCCCAAT+TGG + chr3_2:85966366-85966385 None:intergenic 35.0%
AATTGGGAAGCTCTTATGAA+TGG - chr3_2:85966365-85966384 Msa0433470:CDS 35.0%
GAACAACAAAAGATTCACAG+AGG - chr3_2:85966463-85966482 Msa0433470:CDS 35.0%
TGTTGAGTTACAGCAATTGA+AGG - chr3_2:85966504-85966523 Msa0433470:CDS 35.0%
! TTAGCTTTCTTAACAGGTTC+TGG + chr3_2:85966547-85966566 None:intergenic 35.0%
TGATTAATCAGCAGAAGATG+TGG + chr3_2:85966749-85966768 None:intergenic 35.0%
AATGGGTCAAACAGAAATGA+AGG + chr3_2:85967281-85967300 None:intergenic 35.0%
GGAACAAAAATGCAGAGTGA+GGG + chr3_2:85963528-85963547 None:intergenic 40.0%
TGGAACAAAAATGCAGAGTG+AGG + chr3_2:85963529-85963548 None:intergenic 40.0%
TGGATGAAGGATTCACAGAA+AGG - chr3_2:85963771-85963790 Msa0433470:three_prime_UTR 40.0%
!! ACTGTTCTTTGGGATTTGCT+TGG - chr3_2:85963966-85963985 Msa0433470:three_prime_UTR 40.0%
!! TGGTGCTATTAAGAAAAGGC+TGG - chr3_2:85963986-85964005 Msa0433470:three_prime_UTR 40.0%
!!! TTTGGCTGTTTTGGCTTTTG+AGG - chr3_2:85964082-85964101 Msa0433470:CDS 40.0%
! GAGGTTGTTGCTTACTTTCA+AGG - chr3_2:85964101-85964120 Msa0433470:CDS 40.0%
TTTCCAAAGGTGGTTCCATA+TGG - chr3_2:85964169-85964188 Msa0433470:CDS 40.0%
AAACCATATGGAACCACCTT+TGG + chr3_2:85964175-85964194 None:intergenic 40.0%
!! AACTTCGAAAGTGCCTGAAT+TGG + chr3_2:85964239-85964258 None:intergenic 40.0%
! GACCGTATGCTTCTTTGTTT+CGG - chr3_2:85964290-85964309 Msa0433470:CDS 40.0%
! ACCGTATGCTTCTTTGTTTC+GGG - chr3_2:85964291-85964310 Msa0433470:CDS 40.0%
! ACCCGAAACAAAGAAGCATA+CGG + chr3_2:85964295-85964314 None:intergenic 40.0%
!! TTCTTTGTTTCGGGTGGTTT+TGG - chr3_2:85964300-85964319 Msa0433470:CDS 40.0%
GGTTAAGCCAATGATCAATG+GGG - chr3_2:85964337-85964356 Msa0433470:CDS 40.0%
AATGGATCCCCATTGATCAT+TGG + chr3_2:85964347-85964366 None:intergenic 40.0%
TCCAATGTGCAATGAGAAAG+AGG - chr3_2:85964424-85964443 Msa0433470:CDS 40.0%
ACCTCTTTCTCATTGCACAT+TGG + chr3_2:85964428-85964447 None:intergenic 40.0%
!! TGCATTCCAGTTTTGCTATG+AGG - chr3_2:85964463-85964482 Msa0433470:CDS 40.0%
TGAATCAGTAAACGATCCCT+TGG + chr3_2:85964845-85964864 None:intergenic 40.0%
ACAGTGGTTAATTAAGGCTG+AGG - chr3_2:85964895-85964914 Msa0433470:intron 40.0%
GTCTCTAAGTGGAACCAAAA+GGG - chr3_2:85964917-85964936 Msa0433470:intron 40.0%
GGATAATCCTGAACTAGGTT+TGG - chr3_2:85965198-85965217 Msa0433470:intron 40.0%
TCACAAAAGACCATCTAGCT+TGG + chr3_2:85965225-85965244 None:intergenic 40.0%
CCTGCTGTTCTACTTCAAAA+TGG + chr3_2:85965297-85965316 None:intergenic 40.0%
!!! CCATTTTGAAGTAGAACAGC+AGG - chr3_2:85965294-85965313 Msa0433470:CDS 40.0%
AAGTAGAACAGCAGGTCAAT+GGG - chr3_2:85965302-85965321 Msa0433470:CDS 40.0%
! TTTGGTTTCAATGGAACTGC+TGG - chr3_2:85965340-85965359 Msa0433470:CDS 40.0%
ATCAAAGCCCTAGAAGAATC+TGG - chr3_2:85965373-85965392 Msa0433470:CDS 40.0%
TCAAAGCCCTAGAAGAATCT+GGG - chr3_2:85965374-85965393 Msa0433470:CDS 40.0%
CTGTAAGCTTCATATGACTC+AGG + chr3_2:85965673-85965692 None:intergenic 40.0%
CTGAGTCATATGAAGCTTAC+AGG - chr3_2:85965671-85965690 Msa0433470:intron 40.0%
CACAGCCTAAAAAGTTGCAT+AGG + chr3_2:85965724-85965743 None:intergenic 40.0%
TAGGTGCAGGAAGAATGTTA+AGG + chr3_2:85966258-85966277 None:intergenic 40.0%
GTATCTGGACTCTTCCAATT+GGG - chr3_2:85966349-85966368 Msa0433470:CDS 40.0%
TGGATTGTCACAAAGAAAGC+TGG - chr3_2:85966385-85966404 Msa0433470:CDS 40.0%
TTGTCACAAAGAAAGCTGGA+AGG - chr3_2:85966389-85966408 Msa0433470:CDS 40.0%
AGAAGTCTGTTATCTGCACA+AGG - chr3_2:85966622-85966641 Msa0433470:CDS 40.0%
CAATCAAATCAAGGCCTACA+AGG + chr3_2:85966690-85966709 None:intergenic 40.0%
! CTTGTAGGCCTTGATTTGAT+TGG - chr3_2:85966688-85966707 Msa0433470:CDS 40.0%
TCTGCTCTCCAATCAAATCA+AGG + chr3_2:85966699-85966718 None:intergenic 40.0%
TGGAACGTGAAACACACTAT+TGG + chr3_2:85966773-85966792 None:intergenic 40.0%
ATAGTGTGTTTCACGTTCCA+AGG - chr3_2:85966773-85966792 Msa0433470:CDS 40.0%
ACTGCAACTTATAGTGTGTG+TGG + chr3_2:85966873-85966892 None:intergenic 40.0%
!! CTGTTCTGAGAGTTTCAGAA+AGG + chr3_2:85966939-85966958 None:intergenic 40.0%
CAAGTATTCCATATAGGCAC+TGG - chr3_2:85967050-85967069 Msa0433470:five_prime_UTR 40.0%
! GACATTTGGAATGGTGAGAT+AGG + chr3_2:85967198-85967217 None:intergenic 40.0%
ATGAACTCTGCTCAGACATT+TGG + chr3_2:85967212-85967231 None:intergenic 40.0%
TTCACAGAAAGGAACACCAG+TGG - chr3_2:85963782-85963801 Msa0433470:three_prime_UTR 45.0%
! AACACCAGTGGTGGTGAAAA+TGG - chr3_2:85963794-85963813 Msa0433470:three_prime_UTR 45.0%
ATGGTGCAGATGCTGCATTT+AGG - chr3_2:85963847-85963866 Msa0433470:three_prime_UTR 45.0%
! TTGCCGCGGTTTTTATCTAC+CGG + chr3_2:85963873-85963892 None:intergenic 45.0%
CTTGTTTAGCATTCTTGCCG+CGG + chr3_2:85963887-85963906 None:intergenic 45.0%
!! AAAAGGCTGGTTCGTAGACA+GGG - chr3_2:85963999-85964018 Msa0433470:three_prime_UTR 45.0%
AGGGAGTGGCTGTTGAAAAA+GGG - chr3_2:85964018-85964037 Msa0433470:three_prime_UTR 45.0%
CTACATTGCTCCACCAATTC+AGG - chr3_2:85964223-85964242 Msa0433470:CDS 45.0%
!! TTCGAAAGTGCCTGAATTGG+TGG + chr3_2:85964236-85964255 None:intergenic 45.0%
! ACAAAGAAGCATACGGTCCA+CGG + chr3_2:85964288-85964307 None:intergenic 45.0%
! GTATGCTTCTTTGTTTCGGG+TGG - chr3_2:85964294-85964313 Msa0433470:CDS 45.0%
GATCAATGGGGATCCATTCA+AGG - chr3_2:85964349-85964368 Msa0433470:CDS 45.0%
CTGCAGTGTGTCAAATGGAT+TGG - chr3_2:85964819-85964838 Msa0433470:intron 45.0%
!! TGTGTCAAATGGATTGGCCA+AGG - chr3_2:85964825-85964844 Msa0433470:intron 45.0%
!! GTGTCAAATGGATTGGCCAA+GGG - chr3_2:85964826-85964845 Msa0433470:intron 45.0%
! CTGATGATGAGAGCATACAG+TGG - chr3_2:85964879-85964898 Msa0433470:intron 45.0%
TTAAGGCTGAGGTCTCTAAG+TGG - chr3_2:85964906-85964925 Msa0433470:intron 45.0%
GGTCTCTAAGTGGAACCAAA+AGG - chr3_2:85964916-85964935 Msa0433470:intron 45.0%
CATAATCTATCGGCATCGCT+TGG - chr3_2:85964946-85964965 Msa0433470:intron 45.0%
GTCAGGACTGGATATAAAGC+TGG - chr3_2:85964968-85964987 Msa0433470:intron 45.0%
GCAAACTGTGCCACATTTCA+AGG - chr3_2:85965087-85965106 Msa0433470:CDS 45.0%
! TAGGTTTGGTCCAAGCTAGA+TGG - chr3_2:85965212-85965231 Msa0433470:intron 45.0%
GAAGTAGAACAGCAGGTCAA+TGG - chr3_2:85965301-85965320 Msa0433470:CDS 45.0%
AGTAGAACAGCAGGTCAATG+GGG - chr3_2:85965303-85965322 Msa0433470:CDS 45.0%
TCAATGGAACTGCTGGTGTT+TGG - chr3_2:85965347-85965366 Msa0433470:CDS 45.0%
CAAAGCCCTAGAAGAATCTG+GGG - chr3_2:85965375-85965394 Msa0433470:CDS 45.0%
AAAGCCCTAGAAGAATCTGG+GGG - chr3_2:85965376-85965395 Msa0433470:CDS 45.0%
GAGAGTTCTTCAGGGATCTT+TGG + chr3_2:85965605-85965624 None:intergenic 45.0%
! CAGGGCCTATGCAACTTTTT+AGG - chr3_2:85965716-85965735 Msa0433470:CDS 45.0%
CATGTTGTAGAGCTATAGCG+CGG + chr3_2:85965940-85965959 None:intergenic 45.0%
AGGTGAAGGAGTAAAAGGGA+AGG + chr3_2:85966144-85966163 None:intergenic 45.0%
!! TTCTGCCTCTGGAACAAACA+TGG + chr3_2:85966191-85966210 None:intergenic 45.0%
CATAAAGGAAGTTCTGCCTC+TGG + chr3_2:85966202-85966221 None:intergenic 45.0%
CAGAGGCAGAACTTCCTTTA+TGG - chr3_2:85966200-85966219 Msa0433470:intron 45.0%
GGTATCTGGACTCTTCCAAT+TGG - chr3_2:85966348-85966367 Msa0433470:CDS 45.0%
TGACACAAGCTGTGAGAAGA+AGG + chr3_2:85966603-85966622 None:intergenic 45.0%
CAAGGAAGAATGTCATCCCT+TGG + chr3_2:85966672-85966691 None:intergenic 45.0%
GGGATGACATTCTTCCTTGT+AGG - chr3_2:85966673-85966692 Msa0433470:CDS 45.0%
CTCTGCTCAGACATTTGGAA+TGG + chr3_2:85967207-85967226 None:intergenic 45.0%
ACAGAAAGGAACACCAGTGG+TGG - chr3_2:85963785-85963804 Msa0433470:three_prime_UTR 50.0%
! ACCAGTGGTGGTGAAAATGG+AGG - chr3_2:85963797-85963816 Msa0433470:three_prime_UTR 50.0%
! TCCTCCATTTTCACCACCAC+TGG + chr3_2:85963801-85963820 None:intergenic 50.0%
TGCAGATGCTGCATTTAGGC+CGG - chr3_2:85963851-85963870 Msa0433470:three_prime_UTR 50.0%
AGGCCGGTAGATAAAAACCG+CGG - chr3_2:85963867-85963886 Msa0433470:three_prime_UTR 50.0%
CTTAAAGCTCATCGTGCCGT+TGG - chr3_2:85963924-85963943 Msa0433470:three_prime_UTR 50.0%
! GTGCCGTTGGTTGTGTTACT+TGG - chr3_2:85963937-85963956 Msa0433470:three_prime_UTR 50.0%
!! GAAAAGGCTGGTTCGTAGAC+AGG - chr3_2:85963998-85964017 Msa0433470:three_prime_UTR 50.0%
CAGGGAGTGGCTGTTGAAAA+AGG - chr3_2:85964017-85964036 Msa0433470:three_prime_UTR 50.0%
GCTTGGACCAAACCTAGTTC+AGG + chr3_2:85965208-85965227 None:intergenic 50.0%
TCCAACCATTGAGGTGAGCT+CGG + chr3_2:85965444-85965463 None:intergenic 50.0%
! CGCGCTATAGCTCTACAACA+TGG - chr3_2:85965938-85965957 Msa0433470:CDS 50.0%
! TTTAACTGCAGGTGTCACCG+TGG - chr3_2:85966071-85966090 Msa0433470:intron 50.0%
CGTGTCAGCGCACAATTGAA+TGG + chr3_2:85966835-85966854 None:intergenic 50.0%
!! TCCATATAGGCACTGGTCAC+TGG - chr3_2:85967057-85967076 Msa0433470:five_prime_UTR 50.0%
TCCAGTGACCAGTGCCTATA+TGG + chr3_2:85967061-85967080 None:intergenic 50.0%
GAAGCATACGGTCCACGGAT+TGG + chr3_2:85964283-85964302 None:intergenic 55.0%
TCTATCGGCATCGCTTGGTC+AGG - chr3_2:85964951-85964970 Msa0433470:intron 55.0%
CCAGCCCCCAGATTCTTCTA+GGG + chr3_2:85965383-85965402 None:intergenic 55.0%
TCCGAGCTCACCTCAATGGT+TGG - chr3_2:85965440-85965459 Msa0433470:CDS 55.0%
AGCAACACCGCTGGCATTCA+GGG - chr3_2:85965698-85965717 Msa0433470:CDS 55.0%
CACAGAGGAGCTTCTGACAG+TGG - chr3_2:85966478-85966497 Msa0433470:CDS 55.0%
CAACACGGGTTCAGCATCCT+TGG + chr3_2:85966793-85966812 None:intergenic 55.0%
!! GCTGGTTCGTAGACAGGGAG+TGG - chr3_2:85964004-85964023 Msa0433470:three_prime_UTR 60.0%
CCCTAGAAGAATCTGGGGGC+TGG - chr3_2:85965380-85965399 Msa0433470:CDS 60.0%
GCCAGCCCCCAGATTCTTCT+AGG + chr3_2:85965384-85965403 None:intergenic 60.0%
! AGAAGAATCTGGGGGCTGGC+TGG - chr3_2:85965384-85965403 Msa0433470:CDS 60.0%
GCTGTCCGAGCTCACCTCAA+TGG - chr3_2:85965436-85965455 Msa0433470:CDS 60.0%
ACAGGAAGCAGCAACACCGC+TGG - chr3_2:85965689-85965708 Msa0433470:CDS 60.0%
CAGCAACACCGCTGGCATTC+AGG - chr3_2:85965697-85965716 Msa0433470:CDS 60.0%
GCATAGGCCCTGAATGCCAG+CGG + chr3_2:85965708-85965727 None:intergenic 60.0%
CGGCATCGCTTGGTCAGGAC+TGG - chr3_2:85964956-85964975 Msa0433470:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 85963391 85967391 85963391 ID=Msa0433470;Name=Msa0433470
chr3_2 mRNA 85963391 85967391 85963391 ID=Msa0433470-mRNA-1;Parent=Msa0433470;Name=Msa0433470-mRNA-1;_AED=0.05;_eAED=0.05;_QI=350|1|1|1|1|1|5|665|687
chr3_2 exon 85963391 85964700 85963391 ID=Msa0433470-mRNA-1:exon:24744;Parent=Msa0433470-mRNA-1
chr3_2 exon 85965014 85965127 85965014 ID=Msa0433470-mRNA-1:exon:24743;Parent=Msa0433470-mRNA-1
chr3_2 exon 85965293 85965583 85965293 ID=Msa0433470-mRNA-1:exon:24742;Parent=Msa0433470-mRNA-1
chr3_2 exon 85965674 85965982 85965674 ID=Msa0433470-mRNA-1:exon:24741;Parent=Msa0433470-mRNA-1
chr3_2 exon 85966337 85967391 85966337 ID=Msa0433470-mRNA-1:exon:24740;Parent=Msa0433470-mRNA-1
chr3_2 five_prime_UTR 85967042 85967391 85967042 ID=Msa0433470-mRNA-1:five_prime_utr;Parent=Msa0433470-mRNA-1
chr3_2 CDS 85966337 85967041 85966337 ID=Msa0433470-mRNA-1:cds;Parent=Msa0433470-mRNA-1
chr3_2 CDS 85965674 85965982 85965674 ID=Msa0433470-mRNA-1:cds;Parent=Msa0433470-mRNA-1
chr3_2 CDS 85965293 85965583 85965293 ID=Msa0433470-mRNA-1:cds;Parent=Msa0433470-mRNA-1
chr3_2 CDS 85965014 85965127 85965014 ID=Msa0433470-mRNA-1:cds;Parent=Msa0433470-mRNA-1
chr3_2 CDS 85964056 85964700 85964056 ID=Msa0433470-mRNA-1:cds;Parent=Msa0433470-mRNA-1
chr3_2 three_prime_UTR 85963391 85964055 85963391 ID=Msa0433470-mRNA-1:three_prime_utr;Parent=Msa0433470-mRNA-1
Gene Sequence

>Msa0433470

ATGGCTCCAAGATTGGATTTTTCAAGTTGGTGGATGAAGGATTCACAGAAAGGAACACCAGTGGTGGTGAAAATGGAGGACCCCAATTTCTCAGTTGTGAAGATTAATGGTGCAGATGCTGCATTTAGGCCGGTAGATAAAAACCGCGGCAAGAATGCTAAACAAGTTAGGTGGGTTTTGCTTCTTAAAGCTCATCGTGCCGTTGGTTGTGTTACTTGGTTAGTAACTGTTCTTTGGGATTTGCTTGGTGCTATTAAGAAAAGGCTGGTTCGTAGACAGGGAGTGGCTGTTGAAAAAGGGAAATTGTTGTTTAGAATCATTAGTTTGTTTTTGTTAATTTCTTTGGCTGTTTTGGCTTTTGAGGTTGTTGCTTACTTTCAAGGTTGGCATTTTGGGAATACCAATTTGCACATTCCTCATACTTCTGATTTCCAAAGGTGGTTCCATATGGTTTATGTTGCTTGGTTGACGTTTCGTGCTGACTACATTGCTCCACCAATTCAGGCACTTTCGAAGTTTTGTATTGTTTTGTTTCTTATCCAATCCGTGGACCGTATGCTTCTTTGTTTCGGGTGGTTTTGGATCAAATTCAAGAAGGTTAAGCCAATGATCAATGGGGATCCATTCAAGGTTGATGATGTTGAAGGATCTGTATGCATTTATCCAATGGTTTTGGTTCAAATTCCAATGTGCAATGAGAAAGAGGTGTATGAGCAGTCAATTTCTGCAGTGTGTCAAATGGATTGGCCAAGGGATCGTTTACTGATTCAAGTACTTGACGATTCTGATGATGAGAGCATACAGTGGTTAATTAAGGCTGAGGTCTCTAAGTGGAACCAAAAGGGAATCAACATAATCTATCGGCATCGCTTGGTCAGGACTGGATATAAAGCTGGAAATCTTAATTCTGCAATGAACTGTGACTACGTGAAAGACTATGAATTTGTTGCAATATTTGATGCAGACTTTCAACCAAACCCTGATTTTCTTAAGCAAACTGTGCCACATTTCAAGGATAATCCTGAACTAGGTTTGGTCCAAGCTAGATGGTCTTTTGTGAACAAGGATGAGAATTTGTTGACTCGTCTCCAAAACATAAATTTATGCTTCCATTTTGAAGTAGAACAGCAGGTCAATGGGGTGTTTCTTAATTTCTTTGGTTTCAATGGAACTGCTGGTGTTTGGAGAATCAAAGCCCTAGAAGAATCTGGGGGCTGGCTGGAGAGAACAACTGTAGAAGATATGGACATTGCTGTCCGAGCTCACCTCAATGGTTGGAAATTTATCTTCCTAAATGATGTCAAGGTACTTTGTGAAGTTCCTGAGTCATATGAAGCTTACAGGAAGCAGCAACACCGCTGGCATTCAGGGCCTATGCAACTTTTTAGGCTGTGTCTTCCAGCAATTTTGAAATCTGAGGTGTCACCGTGGAAGAAAGCAAACTTAATTCTGCTATTTTTTCTGCTAAGGAAACTAATCCTTCCCTTTTACTCCTTCACCTTATTTTGCATAATCCTTCCTTTAACCATGTTTGTTCCAGAGGCAGAACTTCCTTTATGGTTGATCTGCTATGTTCCTGTATGCATGTCAATCCTTAACATTCTTCCTGCACCTAAATCTTTTCCTTTTATTGTTCCTTACCTCCTTTTTGAAAACACCATGTCTGTGACTAAATTTAATGCAATGGTATCTGGACTCTTCCAATTGGGAAGCTCTTATGAATGGATTGTCACAAAGAAAGCTGGAAGGTCATCAGAATCTGACTTACTTGCTGCTGCAGAAAGAGAAACCGAGTCAATAGAACAACAAAAGATTCACAGAGGAGCTTCTGACAGTGGACTTGTTGAGTTACAGCAATTGAAGGAACTCAAAGAAGCAGTTCCAGAACCTGTTAAGAAAGCTAACAAGATATATAAAAAAGAGCTGACTTTGGCATTCCTTCTTCTCACAGCTTGTGTCAGAAGTCTGTTATCTGCACAAGGAGTTCATTTCTACTTTCTGTTTTTCCAAGGGATGACATTCTTCCTTGTAGGCCTTGATTTGATTGGAGAGCAGATGAGCTAG
Protein sequence

>Msa0433470

MAPRLDFSSWWMKDSQKGTPVVVKMEDPNFSVVKINGADAAFRPVDKNRGKNAKQVRWVLLLKAHRAVGCVTWLVTVLWDLLGAIKKRLVRRQGVAVEKGKLLFRIISLFLLISLAVLAFEVVAYFQGWHFGNTNLHIPHTSDFQRWFHMVYVAWLTFRADYIAPPIQALSKFCIVLFLIQSVDRMLLCFGWFWIKFKKVKPMINGDPFKVDDVEGSVCIYPMVLVQIPMCNEKEVYEQSISAVCQMDWPRDRLLIQVLDDSDDESIQWLIKAEVSKWNQKGINIIYRHRLVRTGYKAGNLNSAMNCDYVKDYEFVAIFDADFQPNPDFLKQTVPHFKDNPELGLVQARWSFVNKDENLLTRLQNINLCFHFEVEQQVNGVFLNFFGFNGTAGVWRIKALEESGGWLERTTVEDMDIAVRAHLNGWKFIFLNDVKVLCEVPESYEAYRKQQHRWHSGPMQLFRLCLPAILKSEVSPWKKANLILLFFLLRKLILPFYSFTLFCIILPLTMFVPEAELPLWLICYVPVCMSILNILPAPKSFPFIVPYLLFENTMSVTKFNAMVSGLFQLGSSYEWIVTKKAGRSSESDLLAAAERETESIEQQKIHRGASDSGLVELQQLKELKEAVPEPVKKANKIYKKELTLAFLLLTACVRSLLSAQGVHFYFLFFQGMTFFLVGLDLIGEQMS*