AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0477940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0477940 MtrunA17_Chr3g0140381 95.918 147 6 0 12 158 1 147 3.11e-102 289
Msa0477940 MtrunA17_Chr8g0344381 42.593 108 60 2 47 153 30 136 1.60e-24 92.8
Msa0477940 MtrunA17_Chr4g0070351 44.681 94 51 1 47 139 30 123 2.61e-21 84.0
Msa0477940 MtrunA17_Chr5g0394601 42.222 90 48 1 47 136 2 87 6.67e-21 82.0
Msa0477940 MtrunA17_Chr3g0136441 36.111 108 68 1 47 154 11 117 5.50e-19 77.8
Msa0477940 MtrunA17_Chr4g0070371 41.053 95 54 1 47 139 24 118 6.28e-19 78.6
Msa0477940 MtrunA17_Chr1g0150711 35.185 108 66 2 35 139 4 110 5.71e-18 75.9
Msa0477940 MtrunA17_Chr8g0340421 34.146 123 79 2 15 137 31 151 8.50e-18 76.3
Msa0477940 MtrunA17_Chr2g0331111 34.146 123 78 3 15 137 32 151 1.06e-17 76.3
Msa0477940 MtrunA17_Chr1g0205251 37.500 88 50 2 47 133 2 85 1.14e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0477940 AT2G25780.1 37.143 140 86 2 19 156 7 146 1.04e-22 88.6
Msa0477940 AT1G79770.1 39.496 119 67 2 19 137 27 140 4.02e-20 82.0
Msa0477940 AT4G14819.1 41.111 90 47 2 47 136 2 85 3.26e-19 78.2
Msa0477940 AT1G72510.1 38.947 95 57 1 46 139 32 126 3.78e-19 79.7
Msa0477940 AT1G72510.2 38.947 95 57 1 46 139 32 126 3.78e-19 79.7
Msa0477940 AT3G22540.1 37.778 90 52 2 47 136 2 87 3.81e-17 72.8
Msa0477940 AT5G20670.1 40.278 72 43 0 45 116 20 91 2.78e-15 69.3
Msa0477940 AT5G25840.1 31.013 158 85 4 19 156 4 157 5.99e-14 66.2
Msa0477940 AT2G09970.1 35.354 99 56 3 46 139 32 127 7.58e-14 65.9
Msa0477940 AT1G54095.1 37.895 95 54 2 47 138 15 107 3.01e-13 63.9

Find 42 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGGACTAACTTTATATTT+TGG 0.178618 3_3:+82433356 None:intergenic
ACAAAGTTAATGAGAGTTTA+GGG 0.268783 3_3:+82433671 None:intergenic
GACAAAGTTAATGAGAGTTT+AGG 0.302938 3_3:+82433670 None:intergenic
AAAAGGAATTGTAAGGGATT+AGG 0.374238 3_3:-82433616 Msa0477940:CDS
AAGAAGATTATTGTGGAAAG+TGG 0.375021 3_3:-82433822 Msa0477940:CDS
TGATTGTGGCATTATATTCC+TGG 0.387287 3_3:+82433701 None:intergenic
TTTAGACAAAAGGAATTGTA+AGG 0.387538 3_3:-82433623 Msa0477940:CDS
TCTCATTAACTTTGTCTATG+AGG 0.404000 3_3:-82433663 Msa0477940:CDS
CTAAGAGAAGTTTAGACAAA+AGG 0.433359 3_3:-82433633 Msa0477940:CDS
ATGAAAATCAATTAGCCCTA+TGG 0.445372 3_3:+82433562 None:intergenic
CTTTGTATAGGTTTGAATGT+AGG 0.466530 3_3:-82433525 Msa0477940:three_prime_UTR
AAGGCTACGAAAGGCGGTCT+CGG 0.484076 3_3:-82433983 Msa0477940:CDS
CTCATTAACTTTGTCTATGA+GGG 0.486170 3_3:-82433662 Msa0477940:CDS
GGATTCTGAAGGGGTGGAGA+GGG 0.492497 3_3:-82433504 Msa0477940:three_prime_UTR
GAAGCTGTTAAAGATAAAGT+AGG 0.501109 3_3:-82433781 Msa0477940:CDS
CTTCTTTCATCCCACAACAT+TGG 0.506214 3_3:+82433875 None:intergenic
TAGGAGTGCAAGTTATCCAT+AGG 0.508727 3_3:-82433578 Msa0477940:exon
TTTGAATGTAGGATTCTGAA+GGG 0.522384 3_3:-82433514 Msa0477940:three_prime_UTR
AATTAACAAATACTATAACT+TGG 0.525508 3_3:-82433947 Msa0477940:CDS
GTCTCAGATGGATTTCAGCC+AGG 0.530828 3_3:-82433719 Msa0477940:CDS
TTTAGGGTTAAGTCTGATTG+TGG 0.540154 3_3:+82433687 None:intergenic
AATGTAGGATTCTGAAGGGG+TGG 0.540645 3_3:-82433510 Msa0477940:three_prime_UTR
GCACTTACAATAGGAGACCT+TGG 0.548085 3_3:+82435688 None:intergenic
AAGCTGAATGCCAATGTTGT+GGG 0.549736 3_3:-82433885 Msa0477940:CDS
AAGCTGTTAAAGATAAAGTA+GGG 0.550044 3_3:-82433780 Msa0477940:CDS
TGATGCTTTGAAGTCTCAGA+TGG 0.552591 3_3:-82433731 Msa0477940:CDS
GTTTGAATGTAGGATTCTGA+AGG 0.553485 3_3:-82433515 Msa0477940:three_prime_UTR
GAAGTTGAAGAAGATTATTG+TGG 0.554061 3_3:-82433829 Msa0477940:CDS
TTAGACAAAAGGAATTGTAA+GGG 0.554428 3_3:-82433622 Msa0477940:CDS
AGGATTCTGAAGGGGTGGAG+AGG 0.558579 3_3:-82433505 Msa0477940:three_prime_UTR
GACCCTGAGCATGGACGAGA+TGG 0.559776 3_3:-82435719 None:intergenic
TAGGAATAACTAAGCTCACT+AGG 0.561524 3_3:-82433597 Msa0477940:CDS
TATTGTGGAAAGTGGGTGTG+TGG 0.596235 3_3:-82433814 Msa0477940:CDS
AGAAGATTATTGTGGAAAGT+GGG 0.599804 3_3:-82433821 Msa0477940:CDS
GAGTCAGCAAAGGCTACGAA+AGG 0.603985 3_3:-82433992 Msa0477940:CDS
GAAGCTGAATGCCAATGTTG+TGG 0.606985 3_3:-82433886 Msa0477940:CDS
AGGAGTGCAAGTTATCCATA+GGG 0.615137 3_3:-82433577 Msa0477940:exon
TCAGCAAAGGCTACGAAAGG+CGG 0.643815 3_3:-82433989 Msa0477940:CDS
TATAGGAGGAGAGTCAGCAA+AGG 0.654980 3_3:-82434002 Msa0477940:intron
ACGAGATGGGTGAACGACCA+AGG 0.668821 3_3:-82435705 Msa0477940:CDS
TTGAATGTAGGATTCTGAAG+GGG 0.672958 3_3:-82433513 Msa0477940:three_prime_UTR
ACCCTGAGCATGGACGAGAT+GGG 0.685133 3_3:-82435718 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTATTTTTTAAATTA+AGG - chr3_3:82435602-82435621 Msa0477940:intron 0.0%
!!! TATTTTTATTTTTTAAATTA+AGG - chr3_3:82435602-82435621 Msa0477940:intron 0.0%
!!! TTTTTTTTAACAACAAAAAT+TGG + chr3_3:82434065-82434084 None:intergenic 10.0%
!!! TTTTTTTTAACAACAAAAAT+TGG + chr3_3:82434065-82434084 None:intergenic 10.0%
!!! TACTGATCAATTTTTTTTTA+TGG + chr3_3:82433601-82433620 None:intergenic 15.0%
!! CAAAAAAAATATATCTTCAA+TGG + chr3_3:82433802-82433821 None:intergenic 15.0%
!! AAAAGAATCAAAAAACTATA+AGG + chr3_3:82434037-82434056 None:intergenic 15.0%
!! TGAATAAATTAGAGATTAAT+GGG + chr3_3:82434978-82434997 None:intergenic 15.0%
!! ATGAATAAATTAGAGATTAA+TGG + chr3_3:82434979-82434998 None:intergenic 15.0%
!! AATTAACAAATACTATAACT+TGG - chr3_3:82435126-82435145 Msa0477940:intron 15.0%
!!! TACTGATCAATTTTTTTTTA+TGG + chr3_3:82433601-82433620 None:intergenic 15.0%
!! CAAAAAAAATATATCTTCAA+TGG + chr3_3:82433802-82433821 None:intergenic 15.0%
!! AAAAGAATCAAAAAACTATA+AGG + chr3_3:82434037-82434056 None:intergenic 15.0%
!! TGAATAAATTAGAGATTAAT+GGG + chr3_3:82434978-82434997 None:intergenic 15.0%
!! ATGAATAAATTAGAGATTAA+TGG + chr3_3:82434979-82434998 None:intergenic 15.0%
!! AATTAACAAATACTATAACT+TGG - chr3_3:82435126-82435145 Msa0477940:intron 15.0%
!!! TTTTTTTTAGCACTTACAAT+AGG + chr3_3:82433397-82433416 None:intergenic 20.0%
!!! GACTCAAGATTTTTTTTTAT+TGG + chr3_3:82433426-82433445 None:intergenic 20.0%
!! AAAAAAAAATCTTGAGTCTA+CGG - chr3_3:82433428-82433447 Msa0477940:three_prime_UTR 20.0%
!!! AATTTTTTTTTATGGTGGTA+GGG + chr3_3:82433593-82433612 None:intergenic 20.0%
!!! TGATCAATTTTTTTTTATGG+TGG + chr3_3:82433598-82433617 None:intergenic 20.0%
!!! TTTTGGTTGTGAAATTATTA+TGG + chr3_3:82433847-82433866 None:intergenic 20.0%
!! TTAAAAGAGTTTACAAATAG+TGG - chr3_3:82434218-82434237 Msa0477940:intron 20.0%
!!! TTTTTTTTAGCACTTACAAT+AGG + chr3_3:82433397-82433416 None:intergenic 20.0%
!!! GACTCAAGATTTTTTTTTAT+TGG + chr3_3:82433426-82433445 None:intergenic 20.0%
!! AAAAAAAAATCTTGAGTCTA+CGG - chr3_3:82433428-82433447 Msa0477940:three_prime_UTR 20.0%
!!! AATTTTTTTTTATGGTGGTA+GGG + chr3_3:82433593-82433612 None:intergenic 20.0%
!!! TGATCAATTTTTTTTTATGG+TGG + chr3_3:82433598-82433617 None:intergenic 20.0%
!!! TTTTGGTTGTGAAATTATTA+TGG + chr3_3:82433847-82433866 None:intergenic 20.0%
!! TTAAAAGAGTTTACAAATAG+TGG - chr3_3:82434218-82434237 Msa0477940:intron 20.0%
!!! ATTTTTTTTTATGGTGGTAG+GGG + chr3_3:82433592-82433611 None:intergenic 25.0%
!!! CAATTTTTTTTTATGGTGGT+AGG + chr3_3:82433594-82433613 None:intergenic 25.0%
!!! GAGTATTGTTCTTCAATTTT+TGG + chr3_3:82433864-82433883 None:intergenic 25.0%
! AACAATACTCAATAGTACAA+AGG - chr3_3:82433874-82433893 Msa0477940:CDS 25.0%
!! GTACAAAGGTAGTATTTTAT+TGG - chr3_3:82433888-82433907 Msa0477940:CDS 25.0%
!!! TTTTATCTACCTTGCTTTTT+AGG - chr3_3:82434285-82434304 Msa0477940:intron 25.0%
! TATCACATGTATATAGTTGA+AGG + chr3_3:82434514-82434533 None:intergenic 25.0%
! GAAATAACAAACTTTATGCA+TGG + chr3_3:82434605-82434624 None:intergenic 25.0%
!!! CAACTTTTTTCTTTCATTCA+TGG - chr3_3:82434940-82434959 Msa0477940:intron 25.0%
! AATTTCCTGTTTCCATATAT+AGG - chr3_3:82435054-82435073 Msa0477940:intron 25.0%
! AAGCTGTTAAAGATAAAGTA+GGG - chr3_3:82435293-82435312 Msa0477940:intron 25.0%
! ACAAAGTTAATGAGAGTTTA+GGG + chr3_3:82435405-82435424 None:intergenic 25.0%
! TTTAGACAAAAGGAATTGTA+AGG - chr3_3:82435450-82435469 Msa0477940:intron 25.0%
! TTAGACAAAAGGAATTGTAA+GGG - chr3_3:82435451-82435470 Msa0477940:intron 25.0%
!!! TTGTTTTACTGTCTTTGTAT+AGG - chr3_3:82435536-82435555 Msa0477940:intron 25.0%
!!! ATTTTTTTTTATGGTGGTAG+GGG + chr3_3:82433592-82433611 None:intergenic 25.0%
!!! CAATTTTTTTTTATGGTGGT+AGG + chr3_3:82433594-82433613 None:intergenic 25.0%
!!! GAGTATTGTTCTTCAATTTT+TGG + chr3_3:82433864-82433883 None:intergenic 25.0%
! AACAATACTCAATAGTACAA+AGG - chr3_3:82433874-82433893 Msa0477940:CDS 25.0%
!! GTACAAAGGTAGTATTTTAT+TGG - chr3_3:82433888-82433907 Msa0477940:CDS 25.0%
!!! TTTTATCTACCTTGCTTTTT+AGG - chr3_3:82434285-82434304 Msa0477940:intron 25.0%
! TATCACATGTATATAGTTGA+AGG + chr3_3:82434514-82434533 None:intergenic 25.0%
! GAAATAACAAACTTTATGCA+TGG + chr3_3:82434605-82434624 None:intergenic 25.0%
!!! CAACTTTTTTCTTTCATTCA+TGG - chr3_3:82434940-82434959 Msa0477940:intron 25.0%
! AATTTCCTGTTTCCATATAT+AGG - chr3_3:82435054-82435073 Msa0477940:intron 25.0%
! AAGCTGTTAAAGATAAAGTA+GGG - chr3_3:82435293-82435312 Msa0477940:intron 25.0%
! ACAAAGTTAATGAGAGTTTA+GGG + chr3_3:82435405-82435424 None:intergenic 25.0%
! TTTAGACAAAAGGAATTGTA+AGG - chr3_3:82435450-82435469 Msa0477940:intron 25.0%
! TTAGACAAAAGGAATTGTAA+GGG - chr3_3:82435451-82435470 Msa0477940:intron 25.0%
!!! TTGTTTTACTGTCTTTGTAT+AGG - chr3_3:82435536-82435555 Msa0477940:intron 25.0%
GACAATGCATAATATACACA+AGG - chr3_3:82433554-82433573 Msa0477940:three_prime_UTR 30.0%
! AAGGAGTACAAGAATTAGTT+AGG + chr3_3:82434018-82434037 None:intergenic 30.0%
ATGTGTGTGTTTAAATGAGT+TGG - chr3_3:82434439-82434458 Msa0477940:intron 30.0%
! GTTGAAGGTGTATTAAAAAG+AGG + chr3_3:82434499-82434518 None:intergenic 30.0%
TAACAAACTTTATGCATGGA+TGG + chr3_3:82434601-82434620 None:intergenic 30.0%
ATTTATACATTACGTGTGTG+TGG + chr3_3:82434835-82434854 None:intergenic 30.0%
CACACACGTAATGTATAAAT+AGG - chr3_3:82434835-82434854 Msa0477940:intron 30.0%
GAAGTTGAAGAAGATTATTG+TGG - chr3_3:82435244-82435263 Msa0477940:intron 30.0%
AAGAAGATTATTGTGGAAAG+TGG - chr3_3:82435251-82435270 Msa0477940:intron 30.0%
AGAAGATTATTGTGGAAAGT+GGG - chr3_3:82435252-82435271 Msa0477940:intron 30.0%
GAAGCTGTTAAAGATAAAGT+AGG - chr3_3:82435292-82435311 Msa0477940:intron 30.0%
GACAAAGTTAATGAGAGTTT+AGG + chr3_3:82435406-82435425 None:intergenic 30.0%
TCTCATTAACTTTGTCTATG+AGG - chr3_3:82435410-82435429 Msa0477940:intron 30.0%
CTCATTAACTTTGTCTATGA+GGG - chr3_3:82435411-82435430 Msa0477940:intron 30.0%
CTAAGAGAAGTTTAGACAAA+AGG - chr3_3:82435440-82435459 Msa0477940:intron 30.0%
AAAAGGAATTGTAAGGGATT+AGG - chr3_3:82435457-82435476 Msa0477940:intron 30.0%
ATGAAAATCAATTAGCCCTA+TGG + chr3_3:82435514-82435533 None:intergenic 30.0%
CTTTGTATAGGTTTGAATGT+AGG - chr3_3:82435548-82435567 Msa0477940:intron 30.0%
TTTGAATGTAGGATTCTGAA+GGG - chr3_3:82435559-82435578 Msa0477940:intron 30.0%
GACAATGCATAATATACACA+AGG - chr3_3:82433554-82433573 Msa0477940:three_prime_UTR 30.0%
! AAGGAGTACAAGAATTAGTT+AGG + chr3_3:82434018-82434037 None:intergenic 30.0%
ATGTGTGTGTTTAAATGAGT+TGG - chr3_3:82434439-82434458 Msa0477940:intron 30.0%
! GTTGAAGGTGTATTAAAAAG+AGG + chr3_3:82434499-82434518 None:intergenic 30.0%
TAACAAACTTTATGCATGGA+TGG + chr3_3:82434601-82434620 None:intergenic 30.0%
ATTTATACATTACGTGTGTG+TGG + chr3_3:82434835-82434854 None:intergenic 30.0%
CACACACGTAATGTATAAAT+AGG - chr3_3:82434835-82434854 Msa0477940:intron 30.0%
GAAGTTGAAGAAGATTATTG+TGG - chr3_3:82435244-82435263 Msa0477940:intron 30.0%
AAGAAGATTATTGTGGAAAG+TGG - chr3_3:82435251-82435270 Msa0477940:intron 30.0%
AGAAGATTATTGTGGAAAGT+GGG - chr3_3:82435252-82435271 Msa0477940:intron 30.0%
GAAGCTGTTAAAGATAAAGT+AGG - chr3_3:82435292-82435311 Msa0477940:intron 30.0%
GACAAAGTTAATGAGAGTTT+AGG + chr3_3:82435406-82435425 None:intergenic 30.0%
TCTCATTAACTTTGTCTATG+AGG - chr3_3:82435410-82435429 Msa0477940:intron 30.0%
CTCATTAACTTTGTCTATGA+GGG - chr3_3:82435411-82435430 Msa0477940:intron 30.0%
CTAAGAGAAGTTTAGACAAA+AGG - chr3_3:82435440-82435459 Msa0477940:intron 30.0%
AAAAGGAATTGTAAGGGATT+AGG - chr3_3:82435457-82435476 Msa0477940:intron 30.0%
ATGAAAATCAATTAGCCCTA+TGG + chr3_3:82435514-82435533 None:intergenic 30.0%
CTTTGTATAGGTTTGAATGT+AGG - chr3_3:82435548-82435567 Msa0477940:intron 30.0%
TTTGAATGTAGGATTCTGAA+GGG - chr3_3:82435559-82435578 Msa0477940:intron 30.0%
CTTCAATGGAAATCAAAGTG+AGG + chr3_3:82433788-82433807 None:intergenic 35.0%
!!! GTATTTTATTGGTTCGAGCT+TGG - chr3_3:82433899-82433918 Msa0477940:CDS 35.0%
! CTCTGTACTTTGTTTATTGC+AGG - chr3_3:82433937-82433956 Msa0477940:CDS 35.0%
! TACTTTGTTTATTGCAGGTC+TGG - chr3_3:82433942-82433961 Msa0477940:CDS 35.0%
GCAAGGTAGATAAAAGTTTG+AGG + chr3_3:82434280-82434299 None:intergenic 35.0%
AAACTTTATGCATGGATGGA+TGG + chr3_3:82434597-82434616 None:intergenic 35.0%
TTCCTGTTTCCATATATAGG+AGG - chr3_3:82435057-82435076 Msa0477940:intron 35.0%
TGATTGTGGCATTATATTCC+TGG + chr3_3:82435375-82435394 None:intergenic 35.0%
! TTTAGGGTTAAGTCTGATTG+TGG + chr3_3:82435389-82435408 None:intergenic 35.0%
TAGGAATAACTAAGCTCACT+AGG - chr3_3:82435476-82435495 Msa0477940:intron 35.0%
GTTTGAATGTAGGATTCTGA+AGG - chr3_3:82435558-82435577 Msa0477940:intron 35.0%
! TTGAATGTAGGATTCTGAAG+GGG - chr3_3:82435560-82435579 Msa0477940:intron 35.0%
CTTCAATGGAAATCAAAGTG+AGG + chr3_3:82433788-82433807 None:intergenic 35.0%
!!! GTATTTTATTGGTTCGAGCT+TGG - chr3_3:82433899-82433918 Msa0477940:CDS 35.0%
! CTCTGTACTTTGTTTATTGC+AGG - chr3_3:82433937-82433956 Msa0477940:CDS 35.0%
! TACTTTGTTTATTGCAGGTC+TGG - chr3_3:82433942-82433961 Msa0477940:CDS 35.0%
GCAAGGTAGATAAAAGTTTG+AGG + chr3_3:82434280-82434299 None:intergenic 35.0%
AAACTTTATGCATGGATGGA+TGG + chr3_3:82434597-82434616 None:intergenic 35.0%
TTCCTGTTTCCATATATAGG+AGG - chr3_3:82435057-82435076 Msa0477940:intron 35.0%
TGATTGTGGCATTATATTCC+TGG + chr3_3:82435375-82435394 None:intergenic 35.0%
! TTTAGGGTTAAGTCTGATTG+TGG + chr3_3:82435389-82435408 None:intergenic 35.0%
TAGGAATAACTAAGCTCACT+AGG - chr3_3:82435476-82435495 Msa0477940:intron 35.0%
GTTTGAATGTAGGATTCTGA+AGG - chr3_3:82435558-82435577 Msa0477940:intron 35.0%
! TTGAATGTAGGATTCTGAAG+GGG - chr3_3:82435560-82435579 Msa0477940:intron 35.0%
GCATAATATACACAAGGTCC+AGG - chr3_3:82433560-82433579 Msa0477940:exon 40.0%
ATCACTGCTCCTAAAAAGCA+AGG + chr3_3:82434297-82434316 None:intergenic 40.0%
!! TTTTAGGAGCAGTGATTGTG+TGG - chr3_3:82434301-82434320 Msa0477940:intron 40.0%
GAAAGAACAAGTTGTACTGC+TGG + chr3_3:82434627-82434646 None:intergenic 40.0%
CAATGCAAAGGGTTTCTACT+TGG + chr3_3:82434764-82434783 None:intergenic 40.0%
!! ATTGCTAGTGGTGGACAAAT+TGG - chr3_3:82434780-82434799 Msa0477940:intron 40.0%
CTGCTTTGTAAGTCTAGCAA+TGG + chr3_3:82434892-82434911 None:intergenic 40.0%
CATTGCTAGACTTACAAAGC+AGG - chr3_3:82434890-82434909 Msa0477940:intron 40.0%
CTCCTCCTATATATGGAAAC+AGG + chr3_3:82435062-82435081 None:intergenic 40.0%
AAGCTGAATGCCAATGTTGT+GGG - chr3_3:82435188-82435207 Msa0477940:intron 40.0%
CTTCTTTCATCCCACAACAT+TGG + chr3_3:82435201-82435220 None:intergenic 40.0%
!! TGATGCTTTGAAGTCTCAGA+TGG - chr3_3:82435342-82435361 Msa0477940:intron 40.0%
! TAGGAGTGCAAGTTATCCAT+AGG - chr3_3:82435495-82435514 Msa0477940:intron 40.0%
! AGGAGTGCAAGTTATCCATA+GGG - chr3_3:82435496-82435515 Msa0477940:intron 40.0%
GCATAATATACACAAGGTCC+AGG - chr3_3:82433560-82433579 Msa0477940:exon 40.0%
ATCACTGCTCCTAAAAAGCA+AGG + chr3_3:82434297-82434316 None:intergenic 40.0%
!! TTTTAGGAGCAGTGATTGTG+TGG - chr3_3:82434301-82434320 Msa0477940:intron 40.0%
GAAAGAACAAGTTGTACTGC+TGG + chr3_3:82434627-82434646 None:intergenic 40.0%
CAATGCAAAGGGTTTCTACT+TGG + chr3_3:82434764-82434783 None:intergenic 40.0%
!! ATTGCTAGTGGTGGACAAAT+TGG - chr3_3:82434780-82434799 Msa0477940:intron 40.0%
CTGCTTTGTAAGTCTAGCAA+TGG + chr3_3:82434892-82434911 None:intergenic 40.0%
CATTGCTAGACTTACAAAGC+AGG - chr3_3:82434890-82434909 Msa0477940:intron 40.0%
CTCCTCCTATATATGGAAAC+AGG + chr3_3:82435062-82435081 None:intergenic 40.0%
AAGCTGAATGCCAATGTTGT+GGG - chr3_3:82435188-82435207 Msa0477940:intron 40.0%
CTTCTTTCATCCCACAACAT+TGG + chr3_3:82435201-82435220 None:intergenic 40.0%
!! TGATGCTTTGAAGTCTCAGA+TGG - chr3_3:82435342-82435361 Msa0477940:intron 40.0%
! TAGGAGTGCAAGTTATCCAT+AGG - chr3_3:82435495-82435514 Msa0477940:intron 40.0%
! AGGAGTGCAAGTTATCCATA+GGG - chr3_3:82435496-82435515 Msa0477940:intron 40.0%
GCACTTACAATAGGAGACCT+TGG + chr3_3:82433388-82433407 None:intergenic 45.0%
! AGTGGAGTATTTGTGTGCTC+TGG - chr3_3:82434236-82434255 Msa0477940:intron 45.0%
!!! TTTGTGTGCTCTGGTTTTGC+AGG - chr3_3:82434245-82434264 Msa0477940:intron 45.0%
!!! TTGTGTGCTCTGGTTTTGCA+GGG - chr3_3:82434246-82434265 Msa0477940:intron 45.0%
TGCATGATGCATGTGGAACA+AGG + chr3_3:82434651-82434670 None:intergenic 45.0%
TGTTGCATGCATGATGCATG+TGG + chr3_3:82434658-82434677 None:intergenic 45.0%
! CTTGGCTTGATGTGGAACAA+TGG + chr3_3:82434746-82434765 None:intergenic 45.0%
! GGTTTCTACTTGGCTTGATG+TGG + chr3_3:82434754-82434773 None:intergenic 45.0%
GAAACCCTTTGCATTGCTAG+TGG - chr3_3:82434768-82434787 Msa0477940:intron 45.0%
TCCACCACTAGCAATGCAAA+GGG + chr3_3:82434775-82434794 None:intergenic 45.0%
! GCTGACTCTCCTCCTATATA+TGG + chr3_3:82435069-82435088 None:intergenic 45.0%
TATAGGAGGAGAGTCAGCAA+AGG - chr3_3:82435071-82435090 Msa0477940:intron 45.0%
GAAGCTGAATGCCAATGTTG+TGG - chr3_3:82435187-82435206 Msa0477940:intron 45.0%
TATTGTGGAAAGTGGGTGTG+TGG - chr3_3:82435259-82435278 Msa0477940:intron 45.0%
! AATGTAGGATTCTGAAGGGG+TGG - chr3_3:82435563-82435582 Msa0477940:intron 45.0%
GCACTTACAATAGGAGACCT+TGG + chr3_3:82433388-82433407 None:intergenic 45.0%
! AGTGGAGTATTTGTGTGCTC+TGG - chr3_3:82434236-82434255 Msa0477940:intron 45.0%
!!! TTTGTGTGCTCTGGTTTTGC+AGG - chr3_3:82434245-82434264 Msa0477940:intron 45.0%
!!! TTGTGTGCTCTGGTTTTGCA+GGG - chr3_3:82434246-82434265 Msa0477940:intron 45.0%
TGCATGATGCATGTGGAACA+AGG + chr3_3:82434651-82434670 None:intergenic 45.0%
TGTTGCATGCATGATGCATG+TGG + chr3_3:82434658-82434677 None:intergenic 45.0%
! CTTGGCTTGATGTGGAACAA+TGG + chr3_3:82434746-82434765 None:intergenic 45.0%
! GGTTTCTACTTGGCTTGATG+TGG + chr3_3:82434754-82434773 None:intergenic 45.0%
GAAACCCTTTGCATTGCTAG+TGG - chr3_3:82434768-82434787 Msa0477940:intron 45.0%
TCCACCACTAGCAATGCAAA+GGG + chr3_3:82434775-82434794 None:intergenic 45.0%
! GCTGACTCTCCTCCTATATA+TGG + chr3_3:82435069-82435088 None:intergenic 45.0%
TATAGGAGGAGAGTCAGCAA+AGG - chr3_3:82435071-82435090 Msa0477940:intron 45.0%
GAAGCTGAATGCCAATGTTG+TGG - chr3_3:82435187-82435206 Msa0477940:intron 45.0%
TATTGTGGAAAGTGGGTGTG+TGG - chr3_3:82435259-82435278 Msa0477940:intron 45.0%
! AATGTAGGATTCTGAAGGGG+TGG - chr3_3:82435563-82435582 Msa0477940:intron 45.0%
!! AAATTAAAAATAAAATTAAG+TGG - chr3_3:82435637-82435656 Msa0477940:intron 5.0%
!! AATTAAAAATAAAATTAAGT+GGG - chr3_3:82435638-82435657 Msa0477940:intron 5.0%
!! AAATTAAAAATAAAATTAAG+TGG - chr3_3:82435637-82435656 Msa0477940:intron 5.0%
!! AATTAAAAATAAAATTAAGT+GGG - chr3_3:82435638-82435657 Msa0477940:intron 5.0%
TGAGCTTAGCTCAGTTGGCA+GGG - chr3_3:82433532-82433551 Msa0477940:three_prime_UTR 50.0%
!! TGGTTCGAGCTTGGCTTGTT+AGG - chr3_3:82433908-82433927 Msa0477940:CDS 50.0%
GATGGATGGTTGTGTTCTGC+TGG + chr3_3:82434583-82434602 None:intergenic 50.0%
ACCCTTTGCATTGCTAGTGG+TGG - chr3_3:82434771-82434790 Msa0477940:intron 50.0%
GTCCACCACTAGCAATGCAA+AGG + chr3_3:82434776-82434795 None:intergenic 50.0%
GAGTCAGCAAAGGCTACGAA+AGG - chr3_3:82435081-82435100 Msa0477940:intron 50.0%
TCAGCAAAGGCTACGAAAGG+CGG - chr3_3:82435084-82435103 Msa0477940:intron 50.0%
! GTCTCAGATGGATTTCAGCC+AGG - chr3_3:82435354-82435373 Msa0477940:intron 50.0%
TGAGCTTAGCTCAGTTGGCA+GGG - chr3_3:82433532-82433551 Msa0477940:three_prime_UTR 50.0%
!! TGGTTCGAGCTTGGCTTGTT+AGG - chr3_3:82433908-82433927 Msa0477940:CDS 50.0%
GATGGATGGTTGTGTTCTGC+TGG + chr3_3:82434583-82434602 None:intergenic 50.0%
ACCCTTTGCATTGCTAGTGG+TGG - chr3_3:82434771-82434790 Msa0477940:intron 50.0%
GTCCACCACTAGCAATGCAA+AGG + chr3_3:82434776-82434795 None:intergenic 50.0%
GAGTCAGCAAAGGCTACGAA+AGG - chr3_3:82435081-82435100 Msa0477940:intron 50.0%
TCAGCAAAGGCTACGAAAGG+CGG - chr3_3:82435084-82435103 Msa0477940:intron 50.0%
! GTCTCAGATGGATTTCAGCC+AGG - chr3_3:82435354-82435373 Msa0477940:intron 50.0%
ACGAGATGGGTGAACGACCA+AGG - chr3_3:82433368-82433387 Msa0477940:three_prime_UTR 55.0%
CCAACTGAGCTAAGCTCACG+AGG + chr3_3:82433530-82433549 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG - chr3_3:82433527-82433546 Msa0477940:three_prime_UTR 55.0%
GTGAGCTTAGCTCAGTTGGC+AGG - chr3_3:82433531-82433550 Msa0477940:three_prime_UTR 55.0%
TGGTGGTAGGGGTTTGAACC+TGG + chr3_3:82433581-82433600 None:intergenic 55.0%
AAGGCTACGAAAGGCGGTCT+CGG - chr3_3:82435090-82435109 Msa0477940:intron 55.0%
! AGGATTCTGAAGGGGTGGAG+AGG - chr3_3:82435568-82435587 Msa0477940:intron 55.0%
! GGATTCTGAAGGGGTGGAGA+GGG - chr3_3:82435569-82435588 Msa0477940:intron 55.0%
ACGAGATGGGTGAACGACCA+AGG - chr3_3:82433368-82433387 Msa0477940:three_prime_UTR 55.0%
CCAACTGAGCTAAGCTCACG+AGG + chr3_3:82433530-82433549 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG - chr3_3:82433527-82433546 Msa0477940:three_prime_UTR 55.0%
GTGAGCTTAGCTCAGTTGGC+AGG - chr3_3:82433531-82433550 Msa0477940:three_prime_UTR 55.0%
TGGTGGTAGGGGTTTGAACC+TGG + chr3_3:82433581-82433600 None:intergenic 55.0%
AAGGCTACGAAAGGCGGTCT+CGG - chr3_3:82435090-82435109 Msa0477940:intron 55.0%
! AGGATTCTGAAGGGGTGGAG+AGG - chr3_3:82435568-82435587 Msa0477940:intron 55.0%
! GGATTCTGAAGGGGTGGAGA+GGG - chr3_3:82435569-82435588 Msa0477940:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 82433364 82435731 82433364 ID=Msa0477940;Name=Msa0477940
chr3_3 mRNA 82433364 82435731 82433364 ID=Msa0477940-mRNA-1;Parent=Msa0477940;Name=Msa0477940-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0.5|0.5|1|1|2|215|158
chr3_3 exon 82433364 82434019 82433364 ID=Msa0477940-mRNA-1:exon:22941;Parent=Msa0477940-mRNA-1
chr3_3 exon 82435696 82435731 82435696 ID=Msa0477940-mRNA-1:exon:22940;Parent=Msa0477940-mRNA-1
chr3_3 CDS 82435696 82435731 82435696 ID=Msa0477940-mRNA-1:cds;Parent=Msa0477940-mRNA-1
chr3_3 CDS 82433579 82434019 82433579 ID=Msa0477940-mRNA-1:cds;Parent=Msa0477940-mRNA-1
chr3_3 three_prime_UTR 82433364 82433578 82433364 ID=Msa0477940-mRNA-1:three_prime_utr;Parent=Msa0477940-mRNA-1
Gene Sequence

>Msa0477940

ATGGACGAGATGGGTGAACGACCAAGGTCTCCTATTGAGGAGAGTCAGCAAAGGCTACGAAAGGCGGTCTCGGACGTGTCTCAAGAAATTAACAAATACTATAACTTGGAAGTAGAAAGAAAGTTTGATGACATTGAAGAGTTAGAAGAAGCTGAATGCCAATGTTGTGGGATGAAAGAAGAATGTAGCAAAGTTTATATGAGTGAAGTTGAAGAAGATTATTGTGGAAAGTGGGTGTGTGGACTTTGTTGTGAAGCTGTTAAAGATAAAGTAGGGCGAAGTTCTAAAGTTATTACACTTGTTGATGCTTTGAAGTCTCAGATGGATTTCAGCCAGGAATATAATGCCACAATCAGACTTAACCCTAAACTCTCATTAACTTTGTCTATGAGGGATATAGCTAAGAGAAGTTTAGACAAAAGGAATTGTAAGGGATTAGGAATAACTAAGCTCACTAGGAGTGCAAGTTATCCATAG
Protein sequence

>Msa0477940

MDEMGERPRSPIEESQQRLRKAVSDVSQEINKYYNLEVERKFDDIEELEEAECQCCGMKEECSKVYMSEVEEDYCGKWVCGLCCEAVKDKVGRSSKVITLVDALKSQMDFSQEYNATIRLNPKLSLTLSMRDIAKRSLDKRNCKGLGITKLTRSASYP*