Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1463810 | AES73887.1 | 95.238 | 147 | 7 | 0 | 1 | 147 | 1 | 147 | 7.14e-98 | 288 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1463810 | G7J3I0 | 95.238 | 147 | 7 | 0 | 1 | 147 | 1 | 147 | 3.41e-98 | 288 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1455620 | Msa1463810 | 0.813833 | 2.095349e-51 | -8.615850e-47 |
| Msa0663090 | Msa1463810 | 0.806102 | 9.683541e-50 | -8.615850e-47 |
| Msa0706370 | Msa1463810 | 0.814013 | 1.911886e-51 | -8.615850e-47 |
| Msa0165890 | Msa1463810 | 0.816624 | 5.023938e-52 | -8.615850e-47 |
| Msa0208520 | Msa1463810 | 0.820015 | 8.573027e-53 | -8.615850e-47 |
| Msa0226290 | Msa1463810 | 0.816480 | 5.410738e-52 | -8.615850e-47 |
| Msa0383730 | Msa1463810 | 0.889040 | 3.455719e-73 | -8.615850e-47 |
| Msa0477940 | Msa1463810 | 0.848143 | 7.395364e-60 | -8.615850e-47 |
| Msa1172590 | Msa1463810 | 0.814109 | 1.820870e-51 | -8.615850e-47 |
| Msa0522010 | Msa1463810 | 0.847328 | 1.239913e-59 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1463810 | MtrunA17_Chr3g0140381 | 95.238 | 147 | 7 | 0 | 1 | 147 | 1 | 147 | 6.57e-102 | 288 |
| Msa1463810 | MtrunA17_Chr8g0344381 | 41.667 | 108 | 61 | 2 | 36 | 142 | 30 | 136 | 1.21e-24 | 92.8 |
| Msa1463810 | MtrunA17_Chr4g0070351 | 44.681 | 94 | 51 | 1 | 36 | 128 | 30 | 123 | 2.54e-21 | 83.6 |
| Msa1463810 | MtrunA17_Chr5g0394601 | 42.222 | 90 | 48 | 1 | 36 | 125 | 2 | 87 | 4.14e-21 | 82.4 |
| Msa1463810 | MtrunA17_Chr3g0136441 | 35.185 | 108 | 69 | 1 | 36 | 143 | 11 | 117 | 5.54e-19 | 77.4 |
| Msa1463810 | MtrunA17_Chr4g0070371 | 41.053 | 95 | 54 | 1 | 36 | 128 | 24 | 118 | 8.41e-19 | 77.8 |
| Msa1463810 | MtrunA17_Chr2g0331111 | 34.959 | 123 | 77 | 3 | 4 | 126 | 32 | 151 | 1.33e-18 | 78.2 |
| Msa1463810 | MtrunA17_Chr8g0340421 | 34.146 | 123 | 79 | 2 | 4 | 126 | 31 | 151 | 3.02e-18 | 77.0 |
| Msa1463810 | MtrunA17_Chr1g0150711 | 34.259 | 108 | 67 | 2 | 24 | 128 | 4 | 110 | 1.63e-17 | 74.3 |
| Msa1463810 | MtrunA17_Chr1g0205251 | 37.500 | 88 | 50 | 2 | 36 | 122 | 2 | 85 | 9.04e-16 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1463810 | AT2G25780.1 | 36.429 | 140 | 87 | 2 | 8 | 145 | 7 | 146 | 1.43e-22 | 87.8 |
| Msa1463810 | AT1G79770.1 | 38.655 | 119 | 68 | 2 | 8 | 126 | 27 | 140 | 1.72e-19 | 80.1 |
| Msa1463810 | AT4G14819.1 | 41.111 | 90 | 47 | 2 | 36 | 125 | 2 | 85 | 2.59e-19 | 77.8 |
| Msa1463810 | AT1G72510.1 | 37.895 | 95 | 58 | 1 | 35 | 128 | 32 | 126 | 6.73e-19 | 78.6 |
| Msa1463810 | AT1G72510.2 | 37.895 | 95 | 58 | 1 | 35 | 128 | 32 | 126 | 6.73e-19 | 78.6 |
| Msa1463810 | AT3G22540.1 | 37.778 | 90 | 52 | 2 | 36 | 125 | 2 | 87 | 2.69e-17 | 72.8 |
| Msa1463810 | AT5G20670.1 | 38.889 | 72 | 44 | 0 | 34 | 105 | 20 | 91 | 6.25e-15 | 67.8 |
| Msa1463810 | AT5G25840.1 | 31.013 | 158 | 85 | 4 | 8 | 145 | 4 | 157 | 3.28e-14 | 66.6 |
| Msa1463810 | AT1G54095.1 | 37.895 | 95 | 54 | 2 | 36 | 127 | 15 | 107 | 1.54e-13 | 64.3 |
| Msa1463810 | AT2G09970.1 | 34.343 | 99 | 57 | 3 | 35 | 128 | 32 | 127 | 2.08e-13 | 64.3 |
Find 34 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAAATAAAATAAAATAAA+AGG | 0.247141 | tig0043653:-1699 | Msa1463810:intergenic |
| ATGAAAATCAATTAGCCTTA+TGG | 0.272064 | tig0043653:-1524 | Msa1463810:intergenic |
| GACAAAGTTAACGAGAGTTT+AGG | 0.311962 | tig0043653:-1416 | Msa1463810:intergenic |
| CTACTATGAAGTGTTTAAAA+TGG | 0.323218 | tig0043653:+1646 | Msa1463810:three_prime_UTR |
| ACAAAGTTAACGAGAGTTTA+GGG | 0.372573 | tig0043653:-1415 | Msa1463810:intergenic |
| CAAAAGGAATTGTAAGGGAT+TGG | 0.373720 | tig0043653:+1469 | Msa1463810:CDS |
| TGATTGTGGCATTATATTCC+TGG | 0.387287 | tig0043653:-1385 | Msa1463810:intergenic |
| TTTAGACAAAAGGAATTGTA+AGG | 0.387538 | tig0043653:+1463 | Msa1463810:CDS |
| AGCAACACTAGTATTTGAAT+TGG | 0.402414 | tig0043653:+1670 | Msa1463810:three_prime_UTR |
| TCTCGTTAACTTTGTCTATG+AGG | 0.419678 | tig0043653:+1423 | Msa1463810:CDS |
| CTAAGAGAAGTTTAGACAAA+AGG | 0.433359 | tig0043653:+1453 | Msa1463810:CDS |
| AAGATGATTATTGTGGAAAG+TGG | 0.449511 | tig0043653:+1264 | Msa1463810:CDS |
| AAAAGGAATTGTAAGGGATT+GGG | 0.455861 | tig0043653:+1470 | Msa1463810:CDS |
| GTCTTTGTATGTTTGAATGT+AGG | 0.471543 | tig0043653:+1559 | Msa1463810:three_prime_UTR |
| GAAGCTGTTAAAGATAAAGT+AGG | 0.501109 | tig0043653:+1305 | Msa1463810:CDS |
| CTCGTTAACTTTGTCTATGA+GGG | 0.501305 | tig0043653:+1424 | Msa1463810:CDS |
| CTTCTTTCATCCCACAACAT+TGG | 0.506214 | tig0043653:-1211 | Msa1463810:intergenic |
| AATTAACAAATACTATAACT+TGG | 0.525508 | tig0043653:+1139 | Msa1463810:CDS |
| AGGAGTGCAAGTTATCCATA+AGG | 0.525681 | tig0043653:+1509 | Msa1463810:CDS |
| GTCTCAGATGGATTTCAGCC+AGG | 0.530828 | tig0043653:+1367 | Msa1463810:CDS |
| TTTAGGGTTAAGTCTGATTG+TGG | 0.540154 | tig0043653:-1399 | Msa1463810:intergenic |
| AAGCTGAATGCCAATGTTGT+GGG | 0.549736 | tig0043653:+1201 | Msa1463810:CDS |
| AAGCTGTTAAAGATAAAGTA+GGG | 0.550044 | tig0043653:+1306 | Msa1463810:CDS |
| TGATGCTTTGAAGTCTCAGA+TGG | 0.552591 | tig0043653:+1355 | Msa1463810:CDS |
| TTAGACAAAAGGAATTGTAA+GGG | 0.554428 | tig0043653:+1464 | Msa1463810:CDS |
| TGGGAATAACTAAGCTCACT+AGG | 0.561524 | tig0043653:+1489 | Msa1463810:CDS |
| AAGGCTACGAAAGGTGGTCT+CGG | 0.565134 | tig0043653:+1103 | Msa1463810:CDS |
| TATTGTGGAAAGTGGGTGTG+TGG | 0.585148 | tig0043653:+1272 | Msa1463810:CDS |
| GAAGTTGAAGATGATTATTG+TGG | 0.588913 | tig0043653:+1257 | Msa1463810:CDS |
| GAAGCTGAATGCCAATGTTG+TGG | 0.606985 | tig0043653:+1200 | Msa1463810:CDS |
| TCAGCAAAGGCTACGAAAGG+TGG | 0.612007 | tig0043653:+1097 | Msa1463810:CDS |
| GAGTCAGCAAAGGCTACGAA+AGG | 0.612454 | tig0043653:+1094 | Msa1463810:CDS |
| AGATGATTATTGTGGAAAGT+GGG | 0.615123 | tig0043653:+1265 | Msa1463810:CDS |
| TATAGGAGGAGAGTCAGCAA+AGG | 0.654980 | tig0043653:+1084 | Msa1463810:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATAAAATAAAATAAA+AGG | - | tig0043653:1702-1721 | Msa1463810:intergenic | 0.0% |
| !! | ATAAAATAAAATAAAATAAA+AGG | - | tig0043653:1702-1721 | Msa1463810:intergenic | 0.0% |
| !! | TGAATAAATTAGAGATTAAT+GGG | - | tig0043653:991-1010 | Msa1463810:intergenic | 15.0% |
| !! | ATGAATAAATTAGAGATTAA+TGG | - | tig0043653:992-1011 | Msa1463810:intergenic | 15.0% |
| !! | AATTAACAAATACTATAACT+TGG | + | tig0043653:1139-1158 | Msa1463810:CDS | 15.0% |
| !! | TGAATAAATTAGAGATTAAT+GGG | - | tig0043653:991-1010 | Msa1463810:intergenic | 15.0% |
| !! | ATGAATAAATTAGAGATTAA+TGG | - | tig0043653:992-1011 | Msa1463810:intergenic | 15.0% |
| !! | AATTAACAAATACTATAACT+TGG | + | tig0043653:1139-1158 | Msa1463810:CDS | 15.0% |
| !!! | CAACTTTTTTCTTTCATTCA+TGG | + | tig0043653:953-972 | Msa1463810:exon | 25.0% |
| ! | AATTTCCTGTTTCCATATAT+AGG | + | tig0043653:1067-1086 | Msa1463810:intron | 25.0% |
| ! | AAGCTGTTAAAGATAAAGTA+GGG | + | tig0043653:1306-1325 | Msa1463810:CDS | 25.0% |
| ! | TTTAGACAAAAGGAATTGTA+AGG | + | tig0043653:1463-1482 | Msa1463810:CDS | 25.0% |
| ! | TTAGACAAAAGGAATTGTAA+GGG | + | tig0043653:1464-1483 | Msa1463810:CDS | 25.0% |
| !!! | ATGAAAATCAATTAGCCTTA+TGG | - | tig0043653:1527-1546 | Msa1463810:intergenic | 25.0% |
| !! | CTACTATGAAGTGTTTAAAA+TGG | + | tig0043653:1646-1665 | Msa1463810:three_prime_UTR | 25.0% |
| !!! | CAACTTTTTTCTTTCATTCA+TGG | + | tig0043653:953-972 | Msa1463810:exon | 25.0% |
| ! | AATTTCCTGTTTCCATATAT+AGG | + | tig0043653:1067-1086 | Msa1463810:intron | 25.0% |
| ! | AAGCTGTTAAAGATAAAGTA+GGG | + | tig0043653:1306-1325 | Msa1463810:CDS | 25.0% |
| ! | TTTAGACAAAAGGAATTGTA+AGG | + | tig0043653:1463-1482 | Msa1463810:CDS | 25.0% |
| ! | TTAGACAAAAGGAATTGTAA+GGG | + | tig0043653:1464-1483 | Msa1463810:CDS | 25.0% |
| !!! | ATGAAAATCAATTAGCCTTA+TGG | - | tig0043653:1527-1546 | Msa1463810:intergenic | 25.0% |
| !! | CTACTATGAAGTGTTTAAAA+TGG | + | tig0043653:1646-1665 | Msa1463810:three_prime_UTR | 25.0% |
| GAAGTTGAAGATGATTATTG+TGG | + | tig0043653:1257-1276 | Msa1463810:CDS | 30.0% | |
| AAGATGATTATTGTGGAAAG+TGG | + | tig0043653:1264-1283 | Msa1463810:CDS | 30.0% | |
| AGATGATTATTGTGGAAAGT+GGG | + | tig0043653:1265-1284 | Msa1463810:CDS | 30.0% | |
| GAAGCTGTTAAAGATAAAGT+AGG | + | tig0043653:1305-1324 | Msa1463810:CDS | 30.0% | |
| ACAAAGTTAACGAGAGTTTA+GGG | - | tig0043653:1418-1437 | Msa1463810:intergenic | 30.0% | |
| CTAAGAGAAGTTTAGACAAA+AGG | + | tig0043653:1453-1472 | Msa1463810:CDS | 30.0% | |
| AAAAGGAATTGTAAGGGATT+GGG | + | tig0043653:1470-1489 | Msa1463810:CDS | 30.0% | |
| GTCTTTGTATGTTTGAATGT+AGG | + | tig0043653:1559-1578 | Msa1463810:three_prime_UTR | 30.0% | |
| AGCAACACTAGTATTTGAAT+TGG | + | tig0043653:1670-1689 | Msa1463810:three_prime_UTR | 30.0% | |
| GAAGTTGAAGATGATTATTG+TGG | + | tig0043653:1257-1276 | Msa1463810:CDS | 30.0% | |
| AAGATGATTATTGTGGAAAG+TGG | + | tig0043653:1264-1283 | Msa1463810:CDS | 30.0% | |
| AGATGATTATTGTGGAAAGT+GGG | + | tig0043653:1265-1284 | Msa1463810:CDS | 30.0% | |
| GAAGCTGTTAAAGATAAAGT+AGG | + | tig0043653:1305-1324 | Msa1463810:CDS | 30.0% | |
| ACAAAGTTAACGAGAGTTTA+GGG | - | tig0043653:1418-1437 | Msa1463810:intergenic | 30.0% | |
| CTAAGAGAAGTTTAGACAAA+AGG | + | tig0043653:1453-1472 | Msa1463810:CDS | 30.0% | |
| AAAAGGAATTGTAAGGGATT+GGG | + | tig0043653:1470-1489 | Msa1463810:CDS | 30.0% | |
| GTCTTTGTATGTTTGAATGT+AGG | + | tig0043653:1559-1578 | Msa1463810:three_prime_UTR | 30.0% | |
| AGCAACACTAGTATTTGAAT+TGG | + | tig0043653:1670-1689 | Msa1463810:three_prime_UTR | 30.0% | |
| TTCCTGTTTCCATATATAGG+AGG | + | tig0043653:1070-1089 | Msa1463810:intron | 35.0% | |
| TGATTGTGGCATTATATTCC+TGG | - | tig0043653:1388-1407 | Msa1463810:intergenic | 35.0% | |
| ! | TTTAGGGTTAAGTCTGATTG+TGG | - | tig0043653:1402-1421 | Msa1463810:intergenic | 35.0% |
| GACAAAGTTAACGAGAGTTT+AGG | - | tig0043653:1419-1438 | Msa1463810:intergenic | 35.0% | |
| TCTCGTTAACTTTGTCTATG+AGG | + | tig0043653:1423-1442 | Msa1463810:CDS | 35.0% | |
| CTCGTTAACTTTGTCTATGA+GGG | + | tig0043653:1424-1443 | Msa1463810:CDS | 35.0% | |
| CAAAAGGAATTGTAAGGGAT+TGG | + | tig0043653:1469-1488 | Msa1463810:CDS | 35.0% | |
| TTCCTGTTTCCATATATAGG+AGG | + | tig0043653:1070-1089 | Msa1463810:intron | 35.0% | |
| TGATTGTGGCATTATATTCC+TGG | - | tig0043653:1388-1407 | Msa1463810:intergenic | 35.0% | |
| ! | TTTAGGGTTAAGTCTGATTG+TGG | - | tig0043653:1402-1421 | Msa1463810:intergenic | 35.0% |
| GACAAAGTTAACGAGAGTTT+AGG | - | tig0043653:1419-1438 | Msa1463810:intergenic | 35.0% | |
| TCTCGTTAACTTTGTCTATG+AGG | + | tig0043653:1423-1442 | Msa1463810:CDS | 35.0% | |
| CTCGTTAACTTTGTCTATGA+GGG | + | tig0043653:1424-1443 | Msa1463810:CDS | 35.0% | |
| CAAAAGGAATTGTAAGGGAT+TGG | + | tig0043653:1469-1488 | Msa1463810:CDS | 35.0% | |
| CTCCTCCTATATATGGAAAC+AGG | - | tig0043653:1075-1094 | Msa1463810:intergenic | 40.0% | |
| AAGCTGAATGCCAATGTTGT+GGG | + | tig0043653:1201-1220 | Msa1463810:CDS | 40.0% | |
| CTTCTTTCATCCCACAACAT+TGG | - | tig0043653:1214-1233 | Msa1463810:intergenic | 40.0% | |
| !! | TGATGCTTTGAAGTCTCAGA+TGG | + | tig0043653:1355-1374 | Msa1463810:CDS | 40.0% |
| TGGGAATAACTAAGCTCACT+AGG | + | tig0043653:1489-1508 | Msa1463810:CDS | 40.0% | |
| ! | AGGAGTGCAAGTTATCCATA+AGG | + | tig0043653:1509-1528 | Msa1463810:CDS | 40.0% |
| CTCCTCCTATATATGGAAAC+AGG | - | tig0043653:1075-1094 | Msa1463810:intergenic | 40.0% | |
| AAGCTGAATGCCAATGTTGT+GGG | + | tig0043653:1201-1220 | Msa1463810:CDS | 40.0% | |
| CTTCTTTCATCCCACAACAT+TGG | - | tig0043653:1214-1233 | Msa1463810:intergenic | 40.0% | |
| !! | TGATGCTTTGAAGTCTCAGA+TGG | + | tig0043653:1355-1374 | Msa1463810:CDS | 40.0% |
| TGGGAATAACTAAGCTCACT+AGG | + | tig0043653:1489-1508 | Msa1463810:CDS | 40.0% | |
| ! | AGGAGTGCAAGTTATCCATA+AGG | + | tig0043653:1509-1528 | Msa1463810:CDS | 40.0% |
| ! | GCTGACTCTCCTCCTATATA+TGG | - | tig0043653:1082-1101 | Msa1463810:intergenic | 45.0% |
| TATAGGAGGAGAGTCAGCAA+AGG | + | tig0043653:1084-1103 | Msa1463810:intron | 45.0% | |
| GAAGCTGAATGCCAATGTTG+TGG | + | tig0043653:1200-1219 | Msa1463810:CDS | 45.0% | |
| TATTGTGGAAAGTGGGTGTG+TGG | + | tig0043653:1272-1291 | Msa1463810:CDS | 45.0% | |
| ! | GCTGACTCTCCTCCTATATA+TGG | - | tig0043653:1082-1101 | Msa1463810:intergenic | 45.0% |
| TATAGGAGGAGAGTCAGCAA+AGG | + | tig0043653:1084-1103 | Msa1463810:intron | 45.0% | |
| GAAGCTGAATGCCAATGTTG+TGG | + | tig0043653:1200-1219 | Msa1463810:CDS | 45.0% | |
| TATTGTGGAAAGTGGGTGTG+TGG | + | tig0043653:1272-1291 | Msa1463810:CDS | 45.0% | |
| !!! | TTTTAAAAATAAAATTAAGT+TGG | + | tig0043653:1598-1617 | Msa1463810:three_prime_UTR | 5.0% |
| !!! | TTTTAAAAATAAAATTAAGT+TGG | + | tig0043653:1598-1617 | Msa1463810:three_prime_UTR | 5.0% |
| GAGTCAGCAAAGGCTACGAA+AGG | + | tig0043653:1094-1113 | Msa1463810:CDS | 50.0% | |
| TCAGCAAAGGCTACGAAAGG+TGG | + | tig0043653:1097-1116 | Msa1463810:CDS | 50.0% | |
| AAGGCTACGAAAGGTGGTCT+CGG | + | tig0043653:1103-1122 | Msa1463810:CDS | 50.0% | |
| ! | GTCTCAGATGGATTTCAGCC+AGG | + | tig0043653:1367-1386 | Msa1463810:CDS | 50.0% |
| GAGTCAGCAAAGGCTACGAA+AGG | + | tig0043653:1094-1113 | Msa1463810:CDS | 50.0% | |
| TCAGCAAAGGCTACGAAAGG+TGG | + | tig0043653:1097-1116 | Msa1463810:CDS | 50.0% | |
| AAGGCTACGAAAGGTGGTCT+CGG | + | tig0043653:1103-1122 | Msa1463810:CDS | 50.0% | |
| ! | GTCTCAGATGGATTTCAGCC+AGG | + | tig0043653:1367-1386 | Msa1463810:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0043653 | gene | 932 | 1738 | 932 | ID=Msa1463810;Name=Msa1463810 |
| tig0043653 | mRNA | 932 | 1738 | 932 | ID=Msa1463810-mRNA-1;Parent=Msa1463810;Name=Msa1463810-mRNA-1;_AED=0.07;_eAED=0.07;_QI=40|1|1|1|0|0.5|2|209|147 |
| tig0043653 | exon | 932 | 974 | 932 | ID=Msa1463810-mRNA-1:exon:24196;Parent=Msa1463810-mRNA-1 |
| tig0043653 | exon | 1089 | 1738 | 1089 | ID=Msa1463810-mRNA-1:exon:24197;Parent=Msa1463810-mRNA-1 |
| tig0043653 | five_prime_UTR | 932 | 971 | 932 | ID=Msa1463810-mRNA-1:five_prime_utr;Parent=Msa1463810-mRNA-1 |
| tig0043653 | CDS | 972 | 974 | 972 | ID=Msa1463810-mRNA-1:cds;Parent=Msa1463810-mRNA-1 |
| tig0043653 | CDS | 1089 | 1529 | 1089 | ID=Msa1463810-mRNA-1:cds;Parent=Msa1463810-mRNA-1 |
| tig0043653 | three_prime_UTR | 1530 | 1738 | 1530 | ID=Msa1463810-mRNA-1:three_prime_utr;Parent=Msa1463810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
