AlfalfaGEDB | Msa0494400

M. sativa cultivar zhongmu-4 / Msa0494400

KEGG: K15687
GO: -
PFAM: PF18345.4

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0494400	XP_003599125.1	97.723	483	8	2	1	482	1	481	0.0	979

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0494400	sp\|E0X9N4\|C3H69_ARATH	49.593	369	153	9	1	357	1	348	5.92e-108	327

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0494400	G7IWL7	97.723	483	8	2	1	482	1	481	0.0	979

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TFs/TRs:

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Gene ID	Type	Classification
Msa0494400	TF	C3H

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0440230	Msa0494400	0.951615	1.403607e-109	-8.615850e-47
Msa0494210	Msa0494400	0.978556	4.490552e-146	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0494400	MtrunA17_Chr3g0087881	91.304	483	11	2	1	482	1	453	0.0	904
Msa0494400	MtrunA17_Chr3g0087891	52.888	329	126	8	1	307	1	322	5.86e-106	319

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0494400	AT3G08505.1	51.125	311	125	8	1	307	1	288	2.74e-99	301
Msa0494400	AT3G08505.2	51.125	311	125	8	1	307	1	288	2.74e-99	301
Msa0494400	AT3G08505.3	51.125	311	125	8	1	307	1	288	2.74e-99	301

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Find 121 sgRNAs with CRISPR-Local

Find 411 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTCACCATGTCCAAC+AGG	0.444234	3_4:+22282095	Msa0494400:exon
AAAATCTGAGTCCCTCATGA+TGG	0.533480	3_4:-22286921	None:intergenic
AAACCAACCAATCTTTCCAT+AGG	0.507522	3_4:-22287587	None:intergenic
AAACTTACATTCTGATGGCT+TGG	0.395562	3_4:-22283546	None:intergenic
AAATGGAAATTGTCCATTTG+GGG	0.614806	3_4:+22283923	Msa0494400:CDS
AACCAACCAATCTTTCCATA+GGG	0.553971	3_4:-22287586	None:intergenic
AACCCAAACTTACATTCTGA+TGG	0.549424	3_4:-22283551	None:intergenic
AACTTACATTCTGATGGCTT+GGG	0.410250	3_4:-22283545	None:intergenic
AAGATGATGCTTGAGAAGCT+CGG	0.587317	3_4:-22283115	None:intergenic
AAGCCATCAGAATGTAAGTT+TGG	0.301620	3_4:+22283548	Msa0494400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAAAACGATCGAAGAAAAA+AGG	-	chr3_4:22282134-22282153	None:intergenic	30.0%
TCTGATGAGAAAAATCACAT+TGG	-	chr3_4:22282761-22282780	None:intergenic	30.0%
TATCACTAATGTTGATGTGT+CGG	+	chr3_4:22282929-22282948	Msa0494400:intron	30.0%
TGCTAAAGTAGCATCAAATT+GGG	+	chr3_4:22283181-22283200	Msa0494400:CDS	30.0%
TCCTACTGATAAAAAGGAAA+AGG	+	chr3_4:22283418-22283437	Msa0494400:CDS	30.0%
AGGAAAAGGAAAATCATTTG+AGG	+	chr3_4:22283432-22283451	Msa0494400:CDS	30.0%
TACTACAAAGGAAGAAAAAC+AGG	+	chr3_4:22283736-22283755	Msa0494400:CDS	30.0%
GAAATGGAAATTGTCCATTT+GGG	+	chr3_4:22283922-22283941	Msa0494400:CDS	30.0%
AAATGGAAATTGTCCATTTG+GGG	+	chr3_4:22283923-22283942	Msa0494400:CDS	30.0%
AGAACTAATCCAACAAAGAA+AGG	-	chr3_4:22284082-22284101	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	22282030	22287685	22282030	ID=Msa0494400;Name=Msa0494400
chr3_4	mRNA	22282030	22287685	22282030	ID=Msa0494400-mRNA-1;Parent=Msa0494400;Name=Msa0494400-mRNA-1;_AED=0.03;_eAED=0.03;_QI=76\|1\|1\|1\|0.75\|0.8\|5\|352\|482
chr3_4	exon	22282030	22282116	22282030	ID=Msa0494400-mRNA-1:exon:5587;Parent=Msa0494400-mRNA-1
chr3_4	exon	22282716	22282800	22282716	ID=Msa0494400-mRNA-1:exon:5588;Parent=Msa0494400-mRNA-1
chr3_4	exon	22283053	22283789	22283053	ID=Msa0494400-mRNA-1:exon:5589;Parent=Msa0494400-mRNA-1
chr3_4	exon	22283893	22283962	22283893	ID=Msa0494400-mRNA-1:exon:5590;Parent=Msa0494400-mRNA-1
chr3_4	exon	22286788	22287685	22286788	ID=Msa0494400-mRNA-1:exon:5591;Parent=Msa0494400-mRNA-1
chr3_4	five_prime_UTR	22282030	22282105	22282030	ID=Msa0494400-mRNA-1:five_prime_utr;Parent=Msa0494400-mRNA-1
chr3_4	CDS	22282106	22282116	22282106	ID=Msa0494400-mRNA-1:cds;Parent=Msa0494400-mRNA-1
chr3_4	CDS	22282716	22282800	22282716	ID=Msa0494400-mRNA-1:cds;Parent=Msa0494400-mRNA-1
chr3_4	CDS	22283053	22283789	22283053	ID=Msa0494400-mRNA-1:cds;Parent=Msa0494400-mRNA-1
chr3_4	CDS	22283893	22283962	22283893	ID=Msa0494400-mRNA-1:cds;Parent=Msa0494400-mRNA-1
chr3_4	CDS	22286788	22287333	22286788	ID=Msa0494400-mRNA-1:cds;Parent=Msa0494400-mRNA-1
chr3_4	three_prime_UTR	22287334	22287685	22287334	ID=Msa0494400-mRNA-1:three_prime_utr;Parent=Msa0494400-mRNA-1

Gene Sequence

>Msa0494400

ATGTCCAACAGGGTTTGCAAGTTTTATGCGCGAGGCGTATGTTTGAAAGGCGACCAATGTGATTTTTCTCATCAGAGAAAGGACACTGCATCTGATATTTGCTCTTATTATCAGAAAGGGTCTTGTGCTTATGGTAGTCGGTGCAGATATAAGCACGTCCGAGCTTCTCAAGCATCATCTTCAGCATCTATGGTTTCAGATTCTGCTGCGCCTGTGGCTCGTAGTGCTAAAGTAGCATCAAATTGGGTTCCGAAGGTTACAAAGGTTCCTTCACCTGACAAGCGTGGTGTAAAGGGTTTACAACGCAAGCATCAGGACTGTACCGATGTCGGTGAATCCAGTACTGGTAGTGCTAGACCACATGAAAATTTGTTTTGTAAATTTGCTGCTGCCAACTGCCCCGGTGGATGTTCTCGTATTCACGGAAATCAATGCTTGTACTGTAGAAAATATTGCCTGCATCCTACTGATAAAAAGGAAAAGGAAAATCATTTGAGGACTTGTGACAAAAAGGAAAAGTACCTTCTGGCTTTGAAAAACAGCGAAGAGATAGAATGCAATGTTTGTCTCGAGCGTGTCCTTTCGAAACCCAAGCCATCAGAATGTAAGTTTGGGTTGCTTCCAGAATGTGATCACGCTTTCTGTTTGTCATGTATTCGCAATTGGCGTAGTAGTGCCCCAACCTCTGCAATGGAAATCGGTAGTAACACCAATACAGTGAGAACCTGCCCTGTTTGCCGCAAACTATCATATTTTGTCATTCCAAGTGGTATTTGGTTTACTACAAAGGAAGAAAAACAGGAAATTATTGATAACTACAAGGCAAACTGCAGATTGATTGATTGTAAACATTTTGACTCTGGAAATGGAAATTGTCCATTTGGGGCTAGCTGTTTCTACAAGCATACAGTGAAGCCTGGCTCATACACATGGAGACACCGCAGGCCACCACCTCAACGCAGACAAAACCATTTCGATATGCATGACATGTTGGATGTGCTCGGTGAGGTTGATCTATCAAGTGGAGAATTTTATTCCATCATGAGGGACTCAGATTTTTTTGAGGGAATGGATCCATTTGAGATGATGGCATTATCAGATTCGCTGGCTGGCGGTTCAGGTCCTTGTTTGGGTCCTTTTGATTCAGACGATGAGGATGATGGGTTTCGTGTTTCTCAAATTGCTGCTATGCAAGAGGCCATGGCTCTTGGGATGGATGCTTTTGGCCCTGATGACGATGACGATGAGGAAGAGTTTAATGTTTTTCGAATGGCTGCTATGCAAGAGGCCATGGTTTCTGGGATAGATGATTTTGGCCCTGATGATTTTCATGGAATTGATCCTATGGATGCAGCTTTGATATCAATGATGATGCATTCTCATATGGACGACGAAGAAGAAGATGAAGATTACGAGGATGAAGAAGAATACACTGATGATGAATATTAG

Protein sequence

>Msa0494400

MSNRVCKFYARGVCLKGDQCDFSHQRKDTASDICSYYQKGSCAYGSRCRYKHVRASQASSSASMVSDSAAPVARSAKVASNWVPKVTKVPSPDKRGVKGLQRKHQDCTDVGESSTGSARPHENLFCKFAAANCPGGCSRIHGNQCLYCRKYCLHPTDKKEKENHLRTCDKKEKYLLALKNSEEIECNVCLERVLSKPKPSECKFGLLPECDHAFCLSCIRNWRSSAPTSAMEIGSNTNTVRTCPVCRKLSYFVIPSGIWFTTKEEKQEIIDNYKANCRLIDCKHFDSGNGNCPFGASCFYKHTVKPGSYTWRHRRPPPQRRQNHFDMHDMLDVLGEVDLSSGEFYSIMRDSDFFEGMDPFEMMALSDSLAGGSGPCLGPFDSDDEDDGFRVSQIAAMQEAMALGMDAFGPDDDDDEEEFNVFRMAAMQEAMVSGIDDFGPDDFHGIDPMDAALISMMMHSHMDDEEEDEDYEDEEEYTDDEY*