AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0498800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0498800 MtrunA17_Chr3g0096451 98.326 239 4 0 18 256 21 259 4.32e-159 442
Msa0498800 MtrunA17_Chr5g0443331 67.234 235 71 3 18 251 21 250 7.96e-95 279
Msa0498800 MtrunA17_Chr7g0271411 65.882 85 29 0 109 193 160 244 1.80e-34 125
Msa0498800 MtrunA17_Chr2g0304061 61.538 91 34 1 109 199 158 247 4.51e-33 122
Msa0498800 MtrunA17_Chr1g0193131 60.674 89 34 1 100 188 411 498 4.92e-30 118
Msa0498800 MtrunA17_Chr8g0381081 52.475 101 47 1 95 194 43 143 6.68e-29 107
Msa0498800 MtrunA17_Chr1g0177171 43.590 117 56 2 88 194 376 492 2.91e-27 109
Msa0498800 MtrunA17_Chr8g0378361 49.515 103 50 2 107 208 28 129 3.68e-27 103
Msa0498800 MtrunA17_Chr4g0075031 48.148 108 51 2 108 210 381 488 4.28e-27 109
Msa0498800 MtrunA17_Chr8g0380231 62.857 70 26 0 117 186 25 94 2.57e-26 99.0
Msa0498800 MtrunA17_Chr8g0378381 48.421 95 47 2 100 193 23 116 3.96e-25 96.3
Msa0498800 MtrunA17_Chr4g0040911 39.437 142 73 4 109 247 37 168 7.73e-25 97.1
Msa0498800 MtrunA17_Chr8g0378371 49.462 93 44 2 103 195 27 116 1.48e-24 94.7
Msa0498800 MtrunA17_Chr6g0487261 43.802 121 64 3 91 207 351 471 3.31e-24 100
Msa0498800 MtrunA17_Chr4g0034901 48.148 108 54 2 92 197 357 464 1.44e-23 99.0
Msa0498800 MtrunA17_Chr5g0401301 50.000 86 41 2 109 192 329 414 2.89e-23 97.4
Msa0498800 MtrunA17_Chr3g0120401 46.667 90 46 2 107 195 155 243 6.64e-23 94.0
Msa0498800 MtrunA17_Chr3g0121271 41.228 114 66 1 88 200 341 454 2.89e-22 95.1
Msa0498800 MtrunA17_Chr8g0378401 46.809 94 48 2 100 192 23 115 4.94e-22 88.2
Msa0498800 MtrunA17_Chr8g0389741 42.574 101 57 1 93 192 352 452 5.87e-22 94.4
Msa0498800 MtrunA17_Chr5g0447651 46.875 96 47 2 101 192 19 114 6.53e-22 87.8
Msa0498800 MtrunA17_Chr8g0378621 44.681 94 51 1 109 201 364 457 1.50e-21 93.2
Msa0498800 MtrunA17_Chr4g0043511 39.200 125 73 3 76 199 388 510 1.70e-21 93.2
Msa0498800 MtrunA17_Chr5g0439251 48.810 84 42 1 104 186 377 460 6.15e-21 91.3
Msa0498800 MtrunA17_Chr1g0154991 42.857 98 50 3 98 192 9 103 1.41e-20 84.0
Msa0498800 MtrunA17_Chr5g0434591 43.011 93 52 1 104 195 355 447 1.18e-19 87.8
Msa0498800 MtrunA17_Chr1g0161011 38.400 125 72 2 86 209 338 458 1.71e-19 87.4
Msa0498800 MtrunA17_Chr4g0076131 44.186 86 47 1 108 192 27 112 2.06e-19 81.3
Msa0498800 MtrunA17_Chr1g0213651 44.706 85 45 2 109 192 276 359 3.45e-19 85.5
Msa0498800 MtrunA17_Chr8g0339191 41.304 92 53 1 105 195 370 461 1.15e-18 84.7
Msa0498800 MtrunA17_Chr3g0110371 38.710 93 56 1 104 195 356 448 9.10e-18 82.4
Msa0498800 MtrunA17_Chr4g0076141 48.750 80 40 1 108 186 12 91 1.09e-17 76.3
Msa0498800 MtrunA17_Chr7g0234821 37.500 112 68 2 86 196 352 462 1.28e-17 81.6
Msa0498800 MtrunA17_Chr3g0144531 44.037 109 53 5 92 197 356 459 2.61e-17 80.9
Msa0498800 MtrunA17_Chr5g0419121 42.105 95 51 3 104 195 17 110 5.87e-17 77.8
Msa0498800 MtrunA17_Chr4g0043151 46.237 93 47 3 105 195 17 108 8.66e-17 76.3
Msa0498800 MtrunA17_Chr4g0061401 42.105 95 52 3 99 192 14 106 2.25e-15 72.8
Msa0498800 MtrunA17_Chr7g0223961 39.175 97 57 2 96 191 352 447 7.05e-15 73.6
Msa0498800 MtrunA17_Chr2g0313891 36.559 93 56 2 101 192 22 112 5.25e-14 66.6
Msa0498800 MtrunA17_Chr4g0062771 40.625 96 52 4 109 202 374 466 1.61e-12 66.6
Msa0498800 MtrunA17_Chr4g0046311 38.542 96 56 3 92 186 358 451 2.00e-12 66.6
Msa0498800 MtrunA17_Chr3g0138741 38.532 109 60 5 109 213 371 476 7.73e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0498800 AT2G30933.1 50.314 159 74 2 89 242 61 219 3.96e-51 167
Msa0498800 AT2G30933.2 57.576 99 41 1 89 186 61 159 9.01e-36 125
Msa0498800 AT2G30933.3 57.576 99 41 1 89 186 61 159 9.01e-36 125
Msa0498800 AT1G29380.3 65.909 88 30 0 109 196 148 235 2.52e-35 127
Msa0498800 AT1G29380.1 66.667 84 28 0 109 192 148 231 1.24e-34 127
Msa0498800 AT1G09460.1 63.000 100 35 1 100 199 128 225 1.91e-34 126
Msa0498800 AT1G29380.4 69.231 78 24 0 109 186 148 225 6.55e-33 120
Msa0498800 AT1G29380.2 69.231 78 24 0 109 186 148 225 6.55e-33 120
Msa0498800 AT4G05430.1 48.039 102 52 1 100 200 14 115 1.86e-28 105
Msa0498800 AT4G05430.3 50.000 96 47 1 106 200 45 140 2.67e-28 106
Msa0498800 AT5G55180.1 44.167 120 59 2 76 194 346 458 1.20e-27 110
Msa0498800 AT3G58100.1 53.409 88 40 1 109 195 41 128 2.63e-26 101
Msa0498800 AT3G58100.2 53.409 88 40 1 109 195 117 204 7.51e-26 102
Msa0498800 AT2G05790.1 50.000 90 44 1 106 194 381 470 1.32e-25 105
Msa0498800 AT4G26830.2 43.750 112 60 3 85 194 347 457 1.53e-25 104
Msa0498800 AT4G26830.1 43.750 112 60 3 85 194 343 453 1.65e-25 104
Msa0498800 AT1G11820.2 42.143 140 66 4 108 245 380 506 2.60e-25 104
Msa0498800 AT4G05430.2 53.012 83 38 1 105 186 44 126 3.65e-25 97.1
Msa0498800 AT4G13600.1 44.762 105 56 2 107 210 59 162 4.43e-25 99.8
Msa0498800 AT5G55180.2 45.536 112 53 2 76 186 346 450 1.65e-24 102
Msa0498800 AT5G35740.1 47.525 101 49 3 95 192 16 115 6.28e-24 93.6
Msa0498800 AT5G67460.1 48.235 85 43 1 109 192 293 377 2.74e-23 97.8
Msa0498800 AT4G34480.3 47.423 97 48 3 98 193 243 337 2.79e-23 97.8
Msa0498800 AT2G01630.1 46.875 96 50 1 108 202 359 454 4.71e-23 97.8
Msa0498800 AT4G29360.2 38.571 140 78 4 109 247 401 533 5.53e-23 97.8
Msa0498800 AT4G29360.1 38.571 140 78 4 109 247 391 523 5.88e-23 97.8
Msa0498800 AT4G34480.1 47.423 97 48 3 98 193 354 448 6.07e-23 97.8
Msa0498800 AT5G63225.1 45.161 93 49 2 101 192 17 108 7.78e-23 90.5
Msa0498800 AT5G56590.1 50.588 85 41 1 109 192 369 453 8.58e-23 97.4
Msa0498800 AT1G11820.3 55.000 80 35 1 108 186 380 459 3.58e-22 95.5
Msa0498800 AT5G67460.2 50.633 79 38 1 109 186 293 371 6.70e-22 94.0
Msa0498800 AT4G16165.1 44.944 89 48 1 105 192 22 110 1.35e-21 87.0
Msa0498800 AT1G66870.2 41.573 89 51 1 105 192 49 137 1.74e-21 87.8
Msa0498800 AT1G66870.1 41.573 89 51 1 105 192 22 110 3.03e-21 86.3
Msa0498800 AT2G16230.1 40.777 103 59 2 98 199 355 456 1.51e-20 90.9
Msa0498800 AT1G66852.1 40.909 88 52 0 105 192 22 109 2.33e-20 84.0
Msa0498800 AT5G63230.1 41.237 97 55 2 97 192 14 109 2.39e-20 84.0
Msa0498800 AT4G09462.1 40.909 110 63 2 86 194 6 114 8.68e-20 82.4
Msa0498800 AT2G04910.2 44.828 87 47 1 107 192 13 99 1.00e-19 82.0
Msa0498800 AT3G55430.1 42.391 92 51 2 102 192 359 449 1.40e-19 87.8
Msa0498800 AT3G13560.4 48.235 85 43 1 109 192 362 446 2.10e-19 87.4
Msa0498800 AT3G13560.1 48.235 85 43 1 109 192 362 446 2.10e-19 87.4
Msa0498800 AT3G13560.2 48.235 85 43 1 109 192 362 446 2.10e-19 87.4
Msa0498800 AT3G13560.5 48.235 85 43 1 109 192 362 446 2.10e-19 87.4
Msa0498800 AT3G13560.3 48.235 85 43 1 109 192 362 446 2.10e-19 87.4
Msa0498800 AT1G66250.1 47.674 86 44 1 108 192 367 452 2.29e-19 87.4
Msa0498800 AT2G01630.3 48.148 81 41 1 108 187 359 439 2.57e-19 87.0
Msa0498800 AT4G09465.1 41.667 96 54 2 100 194 22 116 2.59e-19 81.3
Msa0498800 AT4G09464.1 41.667 96 54 2 100 194 22 116 2.81e-19 81.3
Msa0498800 AT4G09467.1 41.667 96 54 2 100 194 22 116 2.81e-19 81.3
Msa0498800 AT2G42930.1 46.154 78 41 1 109 186 40 116 2.98e-19 81.6
Msa0498800 AT1G79480.1 43.678 87 47 2 107 192 309 394 3.22e-19 86.3
Msa0498800 AT1G79480.2 43.678 87 47 2 107 192 308 393 3.56e-19 86.3
Msa0498800 AT5G53610.1 39.604 101 58 2 93 192 12 110 3.93e-19 80.5
Msa0498800 AT5G63240.1 42.697 89 48 2 109 194 40 128 4.03e-19 81.3
Msa0498800 AT1G66855.1 39.535 86 51 1 108 192 25 110 4.82e-19 80.5
Msa0498800 AT5G61130.1 45.556 90 47 2 105 193 17 105 9.50e-19 82.0
Msa0498800 AT2G04910.1 46.914 81 42 1 107 186 13 93 1.05e-18 79.7
Msa0498800 AT4G09466.1 41.667 96 54 2 100 194 22 116 1.12e-18 79.7
Msa0498800 AT5G53600.1 39.560 91 52 2 103 192 23 111 1.77e-18 79.0
Msa0498800 AT4G09090.1 38.095 105 63 2 91 194 13 116 3.93e-18 78.2
Msa0498800 AT1G18650.2 39.785 93 54 2 105 196 17 108 5.59e-18 78.2
Msa0498800 AT1G18650.1 39.362 94 55 2 105 197 17 109 6.73e-18 79.3
Msa0498800 AT5G63250.1 35.965 114 65 3 84 194 22 130 7.36e-18 77.8
Msa0498800 AT2G39640.1 39.785 93 54 2 102 193 352 443 2.02e-17 81.6
Msa0498800 AT2G39640.1 36.111 108 67 2 91 197 442 548 1.10e-16 79.7
Msa0498800 AT5G24318.3 35.461 141 69 5 54 192 196 316 4.77e-17 79.7
Msa0498800 AT5G24318.1 35.461 141 69 5 54 192 335 455 9.92e-17 79.7
Msa0498800 AT3G23770.1 37.879 132 68 5 75 196 348 475 2.02e-16 78.6
Msa0498800 AT5G08000.2 47.191 89 45 2 106 193 37 124 4.39e-16 75.1
Msa0498800 AT2G29735.1 39.583 96 54 3 100 192 22 116 6.81e-16 72.4
Msa0498800 AT1G78520.1 41.860 86 47 2 108 192 31 114 1.79e-15 70.9
Msa0498800 AT3G07320.1 37.255 102 63 1 96 196 358 459 3.84e-15 75.1
Msa0498800 AT2G43670.1 40.449 89 50 2 105 192 31 117 5.94e-15 69.7
Msa0498800 AT5G08000.1 46.591 88 45 2 107 193 19 105 6.17e-15 71.6
Msa0498800 AT2G03505.3 38.298 94 54 3 105 195 17 109 2.59e-14 68.2
Msa0498800 AT5G24318.2 34.559 136 67 5 54 187 335 450 5.86e-14 71.2
Msa0498800 AT4G14080.1 36.154 130 73 5 75 196 350 477 6.44e-14 71.2
Msa0498800 AT2G03505.4 38.298 94 54 3 105 195 17 109 7.12e-14 68.2
Msa0498800 AT2G03505.1 38.298 94 54 3 105 195 17 109 7.12e-14 68.2
Msa0498800 AT2G03505.2 38.298 94 54 3 105 195 17 109 7.12e-14 68.2
Msa0498800 AT1G69295.1 44.578 83 43 3 105 186 17 97 8.15e-14 68.9
Msa0498800 AT1G69295.2 43.529 85 45 3 103 186 11 93 1.23e-13 68.6
Msa0498800 AT1G26450.1 42.857 77 41 3 105 180 17 91 5.55e-13 66.2
Msa0498800 AT1G13830.2 41.975 81 44 3 106 185 32 110 1.03e-11 63.2
Msa0498800 AT5G64790.1 30.081 123 75 5 86 200 340 459 2.95e-11 63.5
Msa0498800 AT1G13830.1 41.250 80 44 3 107 185 19 96 6.36e-11 60.5
Msa0498800 AT2G43660.2 33.333 87 57 1 107 192 33 119 8.55e-11 58.5
Msa0498800 AT2G43660.1 33.333 87 57 1 107 192 32 118 9.07e-11 58.5

Find 53 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTAATAGTTTCTCTTTA+TGG 0.062450 3_4:-36103379 None:intergenic
ACAAATTTGCTTCATTATTT+TGG 0.100766 3_4:+36105751 Msa0498800:CDS
GTTCCAAATAGTTGCAATTT+TGG 0.129032 3_4:+36103770 Msa0498800:CDS
CCACCAAAATTGCAACTATT+TGG 0.129616 3_4:-36103773 None:intergenic
GAAATTGTTGGAACTAAATT+TGG 0.162976 3_4:-36103458 None:intergenic
GCATGATCATGAACAGAATT+AGG 0.176113 3_4:-36103710 None:intergenic
AACCTTTGTAGTGTTTAAAT+TGG 0.203502 3_4:+36106011 Msa0498800:three_prime_UTR
TCCCTTTGAGTGGTTGAAAT+TGG 0.224915 3_4:-36103410 None:intergenic
CCTGAAGAAACCGGTAAATT+TGG 0.233291 3_4:-36103563 None:intergenic
TTGGATTATGCTTGTGGATA+TGG 0.271610 3_4:+36103641 Msa0498800:CDS
CCAAATTTACCGGTTTCTTC+AGG 0.317433 3_4:+36103563 Msa0498800:CDS
TCCATCGACGCCAAATTTAC+CGG 0.376279 3_4:+36103553 Msa0498800:CDS
AAATTTGGAATTGTAGTGAT+TGG 0.378876 3_4:-36103443 None:intergenic
ATTGGTGATGTTGAAATTGT+TGG 0.386318 3_4:-36103470 None:intergenic
TAATCTGTGTTTCAGGTGTT+GGG 0.399194 3_4:+36105129 Msa0498800:intron
TTTAGTAGAAATTTATATGA+TGG 0.399832 3_4:+36105949 Msa0498800:three_prime_UTR
ACCGGTAAATTTGGCGTCGA+TGG 0.414573 3_4:-36103554 None:intergenic
GCAGAGACAGATGGGTTTGT+TGG 0.415494 3_4:-36105701 None:intergenic
GAAGATGGAGAATATGTATC+TGG 0.416456 3_4:-36103512 None:intergenic
TATGTATCTGGATTGGAATC+AGG 0.423031 3_4:-36103500 None:intergenic
CATGATCATGAACAGAATTA+GGG 0.428848 3_4:-36103709 None:intergenic
AAATTGCAACTATTTGGAAC+AGG 0.439006 3_4:-36103767 None:intergenic
GGCGTCGATGGAGCAGTAAT+CGG 0.455547 3_4:-36103542 None:intergenic
CAGAGACAGATGGGTTTGTT+GGG 0.455870 3_4:-36105700 None:intergenic
AAGATCTTTACAAGTTGCAT+TGG 0.457574 3_4:+36103622 Msa0498800:CDS
AAAACTCTCATTCTTCATGT+TGG 0.459973 3_4:-36105802 None:intergenic
TTGGAATCAGGATTCAAGAT+TGG 0.462790 3_4:-36103488 None:intergenic
TCCAATTTCAACCACTCAAA+GGG 0.472138 3_4:+36103409 Msa0498800:CDS
TGGAGAATATGTATCTGGAT+TGG 0.497476 3_4:-36103507 None:intergenic
CAACTTGCACCTGAAGAAAC+CGG 0.508981 3_4:-36103572 None:intergenic
CTCCAATTTCAACCACTCAA+AGG 0.519775 3_4:+36103408 Msa0498800:CDS
ACAGATGGGTTTGTTGGGAC+AGG 0.520695 3_4:-36105695 None:intergenic
GTAATCGGCACCGTTGAAGA+TGG 0.526659 3_4:-36103527 None:intergenic
AAAGATCTTTGTGAAGCACT+AGG 0.538067 3_4:-36103608 None:intergenic
GTTGCATTGGATTATGCTTG+TGG 0.554758 3_4:+36103635 Msa0498800:CDS
GGGTTATAACAGCTTCCACC+AGG 0.555203 3_4:-36103689 None:intergenic
GAAGCTGCAGCAGAGACAGA+TGG 0.556193 3_4:-36105710 None:intergenic
AAGCTGCAGCAGAGACAGAT+GGG 0.562564 3_4:-36105709 None:intergenic
AAGATCTTTGTGAAGCACTA+GGG 0.563955 3_4:-36103607 None:intergenic
CGGTTTCTTCAGGTGCAAGT+TGG 0.564585 3_4:+36103573 Msa0498800:CDS
TACTTGGATTTGTGTTAGTG+AGG 0.565803 3_4:-36103805 None:intergenic
AAAGATAGTCACCTTGTGGA+TGG 0.575134 3_4:-36105164 None:intergenic
GATTGTTCTGCAATTCAACC+TGG 0.592178 3_4:+36103671 Msa0498800:CDS
TACATATTCTCCATCTTCAA+CGG 0.603559 3_4:+36103517 Msa0498800:CDS
AGTTGTAATATCCCTTTGAG+TGG 0.607995 3_4:-36103420 None:intergenic
ATCCAATTTAAACACTACAA+AGG 0.609848 3_4:-36106013 None:intergenic
AGCAACTAGCTAGGTACAAG+AGG 0.612214 3_4:+36105898 Msa0498800:three_prime_UTR
TTAAACACAACAAACACAAG+TGG 0.617565 3_4:+36105650 Msa0498800:CDS
TGACTGGAAAGCAACTAGCT+AGG 0.621898 3_4:+36105889 Msa0498800:three_prime_UTR
GATTATGCTTGTGGATATGG+TGG 0.641856 3_4:+36103644 Msa0498800:CDS
CATGTCAATATCCATCCACA+AGG 0.657227 3_4:+36105153 Msa0498800:CDS
TGTTCTGCAATTCAACCTGG+TGG 0.663488 3_4:+36103674 Msa0498800:CDS
GAAACAAATAAAGAAGCACA+TGG 0.689239 3_4:+36103329 Msa0498800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTTTTAAGATTTTTTAA+CGG - chr3_4:36103929-36103948 None:intergenic 10.0%
!!! AATATTTTTATATCTATAGT+AGG - chr3_4:36104157-36104176 None:intergenic 10.0%
!! ACTATAGATATAAAAATATT+TGG + chr3_4:36104157-36104176 Msa0498800:intron 10.0%
!!! TAAAAATGAATAAATTTTGT+AGG + chr3_4:36104885-36104904 Msa0498800:intron 10.0%
!! AAAGGTTAAAAAAAATAATA+TGG - chr3_4:36105998-36106017 None:intergenic 10.0%
!!! TTTTTTCAAAAAGATCAATT+CGG + chr3_4:36104104-36104123 Msa0498800:intron 15.0%
!! AAAAATTGTCACATAATTTA+AGG - chr3_4:36104214-36104233 None:intergenic 15.0%
!!! TCCATCATTTTTATATTTAT+AGG + chr3_4:36104826-36104845 Msa0498800:intron 15.0%
!! TCCTATAAATATAAAAATGA+TGG - chr3_4:36104830-36104849 None:intergenic 15.0%
!!! ATCATTTTTATATTTATAGG+AGG + chr3_4:36104829-36104848 Msa0498800:intron 15.0%
!! GTTAAATATATTTGTAGTTA+TGG - chr3_4:36104937-36104956 None:intergenic 15.0%
!! GAAACTAAAAACATAATATA+TGG + chr3_4:36105394-36105413 Msa0498800:intron 15.0%
!!! AAAATAGTTCTTTTGTTATA+TGG + chr3_4:36105556-36105575 Msa0498800:intron 15.0%
!! TTTAGTAGAAATTTATATGA+TGG + chr3_4:36105949-36105968 Msa0498800:three_prime_UTR 15.0%
!!! AACTGTTTTTCTCAATATAA+AGG + chr3_4:36103299-36103318 Msa0498800:CDS 20.0%
!! ATTCTAATAGTTTCTCTTTA+TGG - chr3_4:36103382-36103401 None:intergenic 20.0%
!!! CTTTTGATAGTACTTATTAA+AGG - chr3_4:36103744-36103763 None:intergenic 20.0%
!!! TATAAAAATATTTGGTGTCA+CGG + chr3_4:36104165-36104184 Msa0498800:intron 20.0%
!! TTATTAATAATCTGTGTTTC+AGG + chr3_4:36105122-36105141 Msa0498800:intron 20.0%
!!! ACAAATTTGCTTCATTATTT+TGG + chr3_4:36105751-36105770 Msa0498800:CDS 20.0%
!!! CTTTGATTTTGTTTTTTTGT+GGG + chr3_4:36105859-36105878 Msa0498800:three_prime_UTR 20.0%
!!! TTTGATTTTGTTTTTTTGTG+GGG + chr3_4:36105860-36105879 Msa0498800:three_prime_UTR 20.0%
! TTGAGAAAAACAGTTGAAAT+AGG - chr3_4:36103294-36103313 None:intergenic 25.0%
!! AAATTTGGAATTGTAGTGAT+TGG - chr3_4:36103446-36103465 None:intergenic 25.0%
! GAAATTGTTGGAACTAAATT+TGG - chr3_4:36103461-36103480 None:intergenic 25.0%
! AAATTATTAGAAGCCTTACT+TGG - chr3_4:36103824-36103843 None:intergenic 25.0%
! GATACTTAATGTTATGATCA+CGG + chr3_4:36103844-36103863 Msa0498800:intron 25.0%
!! TGTTTGATTTCGTATTCATT+TGG + chr3_4:36104047-36104066 Msa0498800:intron 25.0%
!! ATAATTTAAGGTCACAACAA+GGG - chr3_4:36104202-36104221 None:intergenic 25.0%
!! TGCATTGGTGATATATATAT+AGG + chr3_4:36104587-36104606 Msa0498800:intron 25.0%
!!! ATGCAATTGATTTGTATACT+TGG + chr3_4:36104959-36104978 Msa0498800:intron 25.0%
!!! TTGATTTGTATACTTGGTAT+AGG + chr3_4:36104965-36104984 Msa0498800:intron 25.0%
! ATCTTATAAGGTGAATAAGT+GGG - chr3_4:36105509-36105528 None:intergenic 25.0%
! CCACAAAAAAACAAAATCAA+AGG - chr3_4:36105861-36105880 None:intergenic 25.0%
!!! CCTTTGATTTTGTTTTTTTG+TGG + chr3_4:36105858-36105877 Msa0498800:three_prime_UTR 25.0%
! AACCTTTGTAGTGTTTAAAT+TGG + chr3_4:36106011-36106030 Msa0498800:three_prime_UTR 25.0%
! ATCCAATTTAAACACTACAA+AGG - chr3_4:36106016-36106035 None:intergenic 25.0%
! GAAACAAATAAAGAAGCACA+TGG + chr3_4:36103329-36103348 Msa0498800:CDS 30.0%
!! ATTGGTGATGTTGAAATTGT+TGG - chr3_4:36103473-36103492 None:intergenic 30.0%
TACATATTCTCCATCTTCAA+CGG + chr3_4:36103517-36103536 Msa0498800:CDS 30.0%
AAGATCTTTACAAGTTGCAT+TGG + chr3_4:36103622-36103641 Msa0498800:CDS 30.0%
CATGATCATGAACAGAATTA+GGG - chr3_4:36103712-36103731 None:intergenic 30.0%
! AAATTGCAACTATTTGGAAC+AGG - chr3_4:36103770-36103789 None:intergenic 30.0%
! GTTCCAAATAGTTGCAATTT+TGG + chr3_4:36103770-36103789 Msa0498800:CDS 30.0%
AAAATCAAACTAAGCCTATG+TGG - chr3_4:36104075-36104094 None:intergenic 30.0%
! CATAATTTAAGGTCACAACA+AGG - chr3_4:36104203-36104222 None:intergenic 30.0%
GACAAGGGACAATAAAATAA+AGG + chr3_4:36104334-36104353 Msa0498800:intron 30.0%
! AATAGACTTTGAAAGTGCAT+TGG + chr3_4:36104572-36104591 Msa0498800:intron 30.0%
!!! GCTTTTTTCTACTTTATTGC+TGG + chr3_4:36104646-36104665 Msa0498800:intron 30.0%
TTGATTCTTGTCCATATCAA+AGG + chr3_4:36104676-36104695 Msa0498800:intron 30.0%
TAAACAAGACACCTTTGATA+TGG - chr3_4:36104690-36104709 None:intergenic 30.0%
AAAATACCTAAGTCGATACT+AGG - chr3_4:36105067-36105086 None:intergenic 30.0%
!!! ATGGATTAGAGTGTTTCTTT+TGG + chr3_4:36105413-36105432 Msa0498800:intron 30.0%
TCTTATAAGGTGAATAAGTG+GGG - chr3_4:36105508-36105527 None:intergenic 30.0%
CATCTTATAAGGTGAATAAG+TGG - chr3_4:36105510-36105529 None:intergenic 30.0%
CAAGTGACTAACATCTTATA+AGG - chr3_4:36105521-36105540 None:intergenic 30.0%
CTTATAAGATGTTAGTCACT+TGG + chr3_4:36105519-36105538 Msa0498800:intron 30.0%
TTAAACACAACAAACACAAG+TGG + chr3_4:36105650-36105669 Msa0498800:CDS 30.0%
! ACAAGTGGTGCAAATATTTT+TGG + chr3_4:36105665-36105684 Msa0498800:CDS 30.0%
! CAAGTGGTGCAAATATTTTT+GGG + chr3_4:36105666-36105685 Msa0498800:CDS 30.0%
AAAACTCTCATTCTTCATGT+TGG - chr3_4:36105805-36105824 None:intergenic 30.0%
TCCAATTTCAACCACTCAAA+GGG + chr3_4:36103409-36103428 Msa0498800:CDS 35.0%
AGTTGTAATATCCCTTTGAG+TGG - chr3_4:36103423-36103442 None:intergenic 35.0%
TTGGAATCAGGATTCAAGAT+TGG - chr3_4:36103491-36103510 None:intergenic 35.0%
! TATGTATCTGGATTGGAATC+AGG - chr3_4:36103503-36103522 None:intergenic 35.0%
TGGAGAATATGTATCTGGAT+TGG - chr3_4:36103510-36103529 None:intergenic 35.0%
GAAGATGGAGAATATGTATC+TGG - chr3_4:36103515-36103534 None:intergenic 35.0%
! AAGATCTTTGTGAAGCACTA+GGG - chr3_4:36103610-36103629 None:intergenic 35.0%
! AAAGATCTTTGTGAAGCACT+AGG - chr3_4:36103611-36103630 None:intergenic 35.0%
TTGGATTATGCTTGTGGATA+TGG + chr3_4:36103641-36103660 Msa0498800:CDS 35.0%
GCATGATCATGAACAGAATT+AGG - chr3_4:36103713-36103732 None:intergenic 35.0%
CCACCAAAATTGCAACTATT+TGG - chr3_4:36103776-36103795 None:intergenic 35.0%
! CCAAATAGTTGCAATTTTGG+TGG + chr3_4:36103773-36103792 Msa0498800:CDS 35.0%
TACTTGGATTTGTGTTAGTG+AGG - chr3_4:36103808-36103827 None:intergenic 35.0%
CACTAACACAAATCCAAGTA+AGG + chr3_4:36103808-36103827 Msa0498800:intron 35.0%
!!! TCACGGTTTTAAATTGCAGT+CGG + chr3_4:36103861-36103880 Msa0498800:intron 35.0%
GACTAATGTGACTTCAATTG+CGG + chr3_4:36103888-36103907 Msa0498800:intron 35.0%
GCTCGTGATTTAATTCAGAA+AGG - chr3_4:36104131-36104150 None:intergenic 35.0%
AATCATGTGTATCCTTGTGA+AGG + chr3_4:36104288-36104307 Msa0498800:intron 35.0%
TATCCTTGTGAAGGATTCTT+GGG + chr3_4:36104297-36104316 Msa0498800:intron 35.0%
TTGAATGAGAAACACTTGTG+TGG + chr3_4:36104375-36104394 Msa0498800:intron 35.0%
AAACCAAAATTCTTTGGTCC+TGG - chr3_4:36104402-36104421 None:intergenic 35.0%
!!! AGGACCAAAGAATTTTGGTT+TGG + chr3_4:36104401-36104420 Msa0498800:intron 35.0%
GTGACCAAACCAAAATTCTT+TGG - chr3_4:36104408-36104427 None:intergenic 35.0%
AAGAGAGAAGAGAGAGAAAA+TGG - chr3_4:36104473-36104492 None:intergenic 35.0%
CAAAGAGAGAAAATCTTGTG+TGG - chr3_4:36104552-36104571 None:intergenic 35.0%
!! CATTATCCTAGTATCGACTT+AGG + chr3_4:36105058-36105077 Msa0498800:intron 35.0%
! ATAATCTGTGTTTCAGGTGT+TGG + chr3_4:36105128-36105147 Msa0498800:intron 35.0%
! TAATCTGTGTTTCAGGTGTT+GGG + chr3_4:36105129-36105148 Msa0498800:intron 35.0%
GATTAAAGATAGTCACCTTG+TGG - chr3_4:36105171-36105190 None:intergenic 35.0%
TAGAATCAGAGTTCGAACTT+CGG + chr3_4:36105479-36105498 Msa0498800:intron 35.0%
CTCCAATTTCAACCACTCAA+AGG + chr3_4:36103408-36103427 Msa0498800:CDS 40.0%
TCCCTTTGAGTGGTTGAAAT+TGG - chr3_4:36103413-36103432 None:intergenic 40.0%
CCTGAAGAAACCGGTAAATT+TGG - chr3_4:36103566-36103585 None:intergenic 40.0%
!! CCAAATTTACCGGTTTCTTC+AGG + chr3_4:36103563-36103582 Msa0498800:CDS 40.0%
GTTGCATTGGATTATGCTTG+TGG + chr3_4:36103635-36103654 Msa0498800:CDS 40.0%
GATTATGCTTGTGGATATGG+TGG + chr3_4:36103644-36103663 Msa0498800:CDS 40.0%
GATTGTTCTGCAATTCAACC+TGG + chr3_4:36103671-36103690 Msa0498800:CDS 40.0%
ATTGAACCGCACATTGTGAT+TGG + chr3_4:36103950-36103969 Msa0498800:intron 40.0%
GATCTACCAATCACAATGTG+CGG - chr3_4:36103959-36103978 None:intergenic 40.0%
CGAAAAGAGACGAATTGTGA+TGG - chr3_4:36103996-36104015 None:intergenic 40.0%
! GTATTCATTTGGAGCCACAT+AGG + chr3_4:36104058-36104077 Msa0498800:intron 40.0%
GTATCCTTGTGAAGGATTCT+TGG + chr3_4:36104296-36104315 Msa0498800:intron 40.0%
! AGGATTCTTGGGTTCCATTT+TGG + chr3_4:36104308-36104327 Msa0498800:intron 40.0%
! GTTCCATTTTGGACAGACAA+GGG + chr3_4:36104319-36104338 Msa0498800:intron 40.0%
CATGTCAATATCCATCCACA+AGG + chr3_4:36105153-36105172 Msa0498800:CDS 40.0%
AAAGATAGTCACCTTGTGGA+TGG - chr3_4:36105167-36105186 None:intergenic 40.0%
TCCATCGACGCCAAATTTAC+CGG + chr3_4:36103553-36103572 Msa0498800:CDS 45.0%
CAACTTGCACCTGAAGAAAC+CGG - chr3_4:36103575-36103594 None:intergenic 45.0%
TGTTCTGCAATTCAACCTGG+TGG + chr3_4:36103674-36103693 Msa0498800:CDS 45.0%
GAACCCAAGAATCCTTCACA+AGG - chr3_4:36104303-36104322 None:intergenic 45.0%
! GGTTCCATTTTGGACAGACA+AGG + chr3_4:36104318-36104337 Msa0498800:intron 45.0%
TGTCCCTTGTCTGTCCAAAA+TGG - chr3_4:36104325-36104344 None:intergenic 45.0%
! GGACCAGGACCAAAGAATTT+TGG + chr3_4:36104396-36104415 Msa0498800:intron 45.0%
GAGAGAAGAGAAGAGAGAGA+AGG - chr3_4:36104446-36104465 None:intergenic 45.0%
GTGACCAAAGAGAACTTGTG+TGG + chr3_4:36104519-36104538 Msa0498800:intron 45.0%
TGGTCCACACAAGTTCTCTT+TGG - chr3_4:36104526-36104545 None:intergenic 45.0%
AAAGAGAACTTGTGTGGACC+AGG + chr3_4:36104525-36104544 Msa0498800:intron 45.0%
GAGAAAATCTTGTGTGGTCC+TGG - chr3_4:36104546-36104565 None:intergenic 45.0%
! CAGAGACAGATGGGTTTGTT+GGG - chr3_4:36105703-36105722 None:intergenic 45.0%
! TTTTGTGGGGTTTGTGTGAC+TGG + chr3_4:36105873-36105892 Msa0498800:three_prime_UTR 45.0%
TGACTGGAAAGCAACTAGCT+AGG + chr3_4:36105889-36105908 Msa0498800:three_prime_UTR 45.0%
AGCAACTAGCTAGGTACAAG+AGG + chr3_4:36105898-36105917 Msa0498800:three_prime_UTR 45.0%
!!! TTAACTTTTAATTATAAAAA+TGG - chr3_4:36104734-36104753 None:intergenic 5.0%
GTAATCGGCACCGTTGAAGA+TGG - chr3_4:36103530-36103549 None:intergenic 50.0%
! ACCGGTAAATTTGGCGTCGA+TGG - chr3_4:36103557-36103576 None:intergenic 50.0%
CGGTTTCTTCAGGTGCAAGT+TGG + chr3_4:36103573-36103592 Msa0498800:CDS 50.0%
GGGTTATAACAGCTTCCACC+AGG - chr3_4:36103692-36103711 None:intergenic 50.0%
GAGAAACACTTGTGTGGACC+AGG + chr3_4:36104381-36104400 Msa0498800:intron 50.0%
TGGTTCCCGCATAACTCAGT+TGG + chr3_4:36105433-36105452 Msa0498800:intron 50.0%
TCCTGCCAACTGAGTTATGC+GGG - chr3_4:36105441-36105460 None:intergenic 50.0%
GTCCTGCCAACTGAGTTATG+CGG - chr3_4:36105442-36105461 None:intergenic 50.0%
!! ACAGATGGGTTTGTTGGGAC+AGG - chr3_4:36105698-36105717 None:intergenic 50.0%
! GCAGAGACAGATGGGTTTGT+TGG - chr3_4:36105704-36105723 None:intergenic 50.0%
AAGCTGCAGCAGAGACAGAT+GGG - chr3_4:36105712-36105731 None:intergenic 50.0%
!! GGCGTCGATGGAGCAGTAAT+CGG - chr3_4:36103545-36103564 None:intergenic 55.0%
TCCCGCATAACTCAGTTGGC+AGG + chr3_4:36105437-36105456 Msa0498800:intron 55.0%
GAAGCTGCAGCAGAGACAGA+TGG - chr3_4:36105713-36105732 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 36103269 36106041 36103269 ID=Msa0498800;Name=Msa0498800
chr3_4 mRNA 36103269 36106041 36103269 ID=Msa0498800-mRNA-1;Parent=Msa0498800;Name=Msa0498800-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|1|0.66|1|0|0.33|3|224|256
chr3_4 exon 36103269 36103824 36103269 ID=Msa0498800-mRNA-1:exon:7595;Parent=Msa0498800-mRNA-1
chr3_4 exon 36105144 36105174 36105144 ID=Msa0498800-mRNA-1:exon:7596;Parent=Msa0498800-mRNA-1
chr3_4 exon 36105634 36106041 36105634 ID=Msa0498800-mRNA-1:exon:7597;Parent=Msa0498800-mRNA-1
chr3_4 CDS 36103269 36103824 36103269 ID=Msa0498800-mRNA-1:cds;Parent=Msa0498800-mRNA-1
chr3_4 CDS 36105144 36105174 36105144 ID=Msa0498800-mRNA-1:cds;Parent=Msa0498800-mRNA-1
chr3_4 CDS 36105634 36105817 36105634 ID=Msa0498800-mRNA-1:cds;Parent=Msa0498800-mRNA-1
chr3_4 three_prime_UTR 36105818 36106041 36105818 ID=Msa0498800-mRNA-1:three_prime_utr;Parent=Msa0498800-mRNA-1
Gene Sequence

>Msa0498800

ATGCTTAGAACAAGACAAATAACCTATTTCAACTGTTTTTCTCAATATAAAGGTTACAGTGAAACAAATAAAGAAGCACATGGACATGTTACACAAGTAAATCTACAAAGCCATAAAGAGAAACTATTAGAATTTAAATCTCCAATTTCAACCACTCAAAGGGATATTACAACTCCAATCACTACAATTCCAAATTTAGTTCCAACAATTTCAACATCACCAATCTTGAATCCTGATTCCAATCCAGATACATATTCTCCATCTTCAACGGTGCCGATTACTGCTCCATCGACGCCAAATTTACCGGTTTCTTCAGGTGCAAGTTGGTGCATTGCAAGCCCTAGTGCTTCACAAAGATCTTTACAAGTTGCATTGGATTATGCTTGTGGATATGGTGGTACTGATTGTTCTGCAATTCAACCTGGTGGAAGCTGTTATAACCCTAATTCTGTTCATGATCATGCTTCTTTTGCCTTTAATAAGTACTATCAAAAGAATCCTGTTCCAAATAGTTGCAATTTTGGTGGAAATGCTGTCCTCACTAACACAAATCCAAGTGTTGGGACATGTCAATATCCATCCACAAGTACAAGTTCATCACTCTTAAACACAACAAACACAAGTGGTGCAAATATTTTTGGGTACGTACCTGTCCCAACAAACCCATCTGTCTCTGCTGCAGCTTCAACATCAAATAGCTTTGCACAAATTTGCTTCATTATTTTGGCAATTGCAATTTTGCAAAACAAGTATATCCAACATGAAGAATGA
Protein sequence

>Msa0498800

MLRTRQITYFNCFSQYKGYSETNKEAHGHVTQVNLQSHKEKLLEFKSPISTTQRDITTPITTIPNLVPTISTSPILNPDSNPDTYSPSSTVPITAPSTPNLPVSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQKNPVPNSCNFGGNAVLTNTNPSVGTCQYPSTSTSSSLLNTTNTSGANIFGYVPVPTNPSVSAAASTSNSFAQICFIILAIAILQNKYIQHEE*