AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0501800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0501800 MtrunA17_Chr5g0393651 84.211 38 6 0 43 80 105 142 3.18e-15 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAACTTGCAATCATTTA+AGG 0.105664 3_4:+43370549 None:intergenic
CGAATACGCCTCTTGGTTTC+AGG 0.119123 3_4:+43370656 None:intergenic
GTGTACGGAACTGTTATATT+TGG 0.220852 3_4:-43370482 Msa0501800:three_prime_UTR
TTTATATGGAGGCCACATTT+TGG 0.338299 3_4:-43370504 Msa0501800:three_prime_UTR
GTTGTTATGCTTGAGATTGC+TGG 0.372211 3_4:-43370590 Msa0501800:CDS
GAATACGCCTCTTGGTTTCA+GGG 0.469031 3_4:+43370657 None:intergenic
GCCACTGTCTCTCCCAGCGG+CGG 0.480179 3_4:-43371879 Msa0501800:CDS
TTGTTATGCTTGAGATTGCT+GGG 0.483156 3_4:-43370589 Msa0501800:CDS
ATCACAGCGAATACGCCTCT+TGG 0.502139 3_4:+43370649 None:intergenic
CCCAGCGGCGGGAAAATTCG+CGG 0.505202 3_4:-43371867 Msa0501800:intron
CCACTGTCTCTCCCAGCGGC+GGG 0.525742 3_4:-43371878 Msa0501800:CDS
CTGTGATGAGAAGCTGAAGA+AGG 0.538208 3_4:-43370633 Msa0501800:CDS
ACAAACCTTGGTGGTTGTTG+AGG 0.544776 3_4:+43371957 None:intergenic
GCTGAAGAAGGTCTTTGCAT+GGG 0.550797 3_4:-43370621 Msa0501800:CDS
GCAATCATTTAAGGAAGTGA+GGG 0.561355 3_4:+43370558 None:intergenic
TGGGTTGCATCTCGTAGGTG+TGG 0.564885 3_4:+43371926 None:intergenic
AGCTGAAGAAGGTCTTTGCA+TGG 0.568943 3_4:-43370622 Msa0501800:CDS
TGCAATCATTTAAGGAAGTG+AGG 0.569687 3_4:+43370557 None:intergenic
TAACAACTGTTGTTCAGAGT+GGG 0.569888 3_4:+43371907 None:intergenic
CCCGCCGCTGGGAGAGACAG+TGG 0.570682 3_4:+43371878 None:intergenic
TTATTAAGGATGATACCAGC+AGG 0.584909 3_4:-43372286 None:intergenic
GGGTTGCATCTCGTAGGTGT+GGG 0.605485 3_4:+43371927 None:intergenic
GTGGGAAACGCAACAAACCT+TGG 0.619598 3_4:+43371945 None:intergenic
CAGAGTGGGTTGCATCTCGT+AGG 0.627246 3_4:+43371921 None:intergenic
CTGAAGAAGGTCTTTGCATG+GGG 0.633482 3_4:-43370620 Msa0501800:CDS
AAGTATCGTGTCATACAACA+TGG 0.665631 3_4:-43370459 Msa0501800:three_prime_UTR
GTAACAACTGTTGTTCAGAG+TGG 0.669335 3_4:+43371906 None:intergenic
CAGTTCCGTACACCAAAATG+TGG 0.671922 3_4:+43370492 None:intergenic
GGAAACGCAACAAACCTTGG+TGG 0.675533 3_4:+43371948 None:intergenic
CACAGGACCCTGAAACCAAG+AGG 0.678850 3_4:-43370664 Msa0501800:intron
ACAGGCCTCAACAACCACCA+AGG 0.713966 3_4:-43371962 Msa0501800:intron
TCTGCCACTGTCTCTCCCAG+CGG 0.721594 3_4:-43371882 Msa0501800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAATAAAATATGAAGAA+TGG + chr3_4:43371348-43371367 None:intergenic 10.0%
!! TAAAAATAAAATATGAAGAA+TGG + chr3_4:43371348-43371367 None:intergenic 10.0%
!! ATTGAAGTTTCAAAATTTAA+AGG - chr3_4:43370513-43370532 Msa0501800:three_prime_UTR 15.0%
!! TTGAAGTTTCAAAATTTAAA+GGG - chr3_4:43370514-43370533 Msa0501800:three_prime_UTR 15.0%
!!! TTTTTTTTTTTTGGTCAAAA+GGG + chr3_4:43371052-43371071 None:intergenic 15.0%
!!! TTTTTTTTTTTTTGGTCAAA+AGG + chr3_4:43371053-43371072 None:intergenic 15.0%
!! TCAAAAACAAATTATTCTTA+GGG + chr3_4:43371097-43371116 None:intergenic 15.0%
!! TTCAAAAACAAATTATTCTT+AGG + chr3_4:43371098-43371117 None:intergenic 15.0%
!!! TCTTCATATTTTATTTTTAG+TGG - chr3_4:43371349-43371368 Msa0501800:intron 15.0%
!! GTCTACAATATAAAAAATTA+GGG + chr3_4:43371452-43371471 None:intergenic 15.0%
!! TGTCTACAATATAAAAAATT+AGG + chr3_4:43371453-43371472 None:intergenic 15.0%
!! ATTTGTAATTATATCAAACA+TGG + chr3_4:43371852-43371871 None:intergenic 15.0%
!! ATTGAAGTTTCAAAATTTAA+AGG - chr3_4:43370513-43370532 Msa0501800:three_prime_UTR 15.0%
!! TTGAAGTTTCAAAATTTAAA+GGG - chr3_4:43370514-43370533 Msa0501800:three_prime_UTR 15.0%
!!! TTTTTTTTTTTTGGTCAAAA+GGG + chr3_4:43371052-43371071 None:intergenic 15.0%
!!! TTTTTTTTTTTTTGGTCAAA+AGG + chr3_4:43371053-43371072 None:intergenic 15.0%
!! TCAAAAACAAATTATTCTTA+GGG + chr3_4:43371097-43371116 None:intergenic 15.0%
!! TTCAAAAACAAATTATTCTT+AGG + chr3_4:43371098-43371117 None:intergenic 15.0%
!!! TCTTCATATTTTATTTTTAG+TGG - chr3_4:43371349-43371368 Msa0501800:intron 15.0%
!! GTCTACAATATAAAAAATTA+GGG + chr3_4:43371452-43371471 None:intergenic 15.0%
!! TGTCTACAATATAAAAAATT+AGG + chr3_4:43371453-43371472 None:intergenic 15.0%
!! ATTTGTAATTATATCAAACA+TGG + chr3_4:43371852-43371871 None:intergenic 15.0%
!!! ATTCTTTTCATTATCTTATG+AGG - chr3_4:43371198-43371217 Msa0501800:intron 20.0%
!! TGTTATAAACTAAATTGTGT+CGG + chr3_4:43371408-43371427 None:intergenic 20.0%
!! TAAAAAATTAGGGACTAATT+CGG + chr3_4:43371442-43371461 None:intergenic 20.0%
!!! AATATTTTTGGAAAAGTTGT+GGG + chr3_4:43371615-43371634 None:intergenic 20.0%
!!! AAATATTTTTGGAAAAGTTG+TGG + chr3_4:43371616-43371635 None:intergenic 20.0%
!!! TATCACAGTCAAAATATTTT+TGG + chr3_4:43371627-43371646 None:intergenic 20.0%
!!! CATTTAATTTAAGCATAACT+CGG + chr3_4:43371938-43371957 None:intergenic 20.0%
!!! TGCTTAAATTAAATGTTTTC+TGG - chr3_4:43371943-43371962 Msa0501800:CDS 20.0%
!!! GCTTAAATTAAATGTTTTCT+GGG - chr3_4:43371944-43371963 Msa0501800:CDS 20.0%
!!! ATTCTTTTCATTATCTTATG+AGG - chr3_4:43371198-43371217 Msa0501800:intron 20.0%
!! TGTTATAAACTAAATTGTGT+CGG + chr3_4:43371408-43371427 None:intergenic 20.0%
!! TAAAAAATTAGGGACTAATT+CGG + chr3_4:43371442-43371461 None:intergenic 20.0%
!!! AATATTTTTGGAAAAGTTGT+GGG + chr3_4:43371615-43371634 None:intergenic 20.0%
!!! AAATATTTTTGGAAAAGTTG+TGG + chr3_4:43371616-43371635 None:intergenic 20.0%
!!! TATCACAGTCAAAATATTTT+TGG + chr3_4:43371627-43371646 None:intergenic 20.0%
!!! CATTTAATTTAAGCATAACT+CGG + chr3_4:43371938-43371957 None:intergenic 20.0%
!!! TGCTTAAATTAAATGTTTTC+TGG - chr3_4:43371943-43371962 Msa0501800:CDS 20.0%
!!! GCTTAAATTAAATGTTTTCT+GGG - chr3_4:43371944-43371963 Msa0501800:CDS 20.0%
!!! AAGATTGTGTTACTTAATGT+AGG + chr3_4:43370986-43371005 None:intergenic 25.0%
! TAATTCATTCATTTCTCTTC+TGG - chr3_4:43371016-43371035 Msa0501800:intron 25.0%
! TTAAGTTCATTCAGAATGTT+TGG - chr3_4:43371136-43371155 Msa0501800:intron 25.0%
! GTTATAAACTAAATTGTGTC+GGG + chr3_4:43371407-43371426 None:intergenic 25.0%
! AAAAAATTAGGGACTAATTC+GGG + chr3_4:43371441-43371460 None:intergenic 25.0%
! TTTATATTGTAGACAAGATC+AGG - chr3_4:43371458-43371477 Msa0501800:intron 25.0%
! TCCTCAATATGAAAATGTTA+GGG - chr3_4:43371491-43371510 Msa0501800:intron 25.0%
! AATATGAAAATGTTAGGGTT+TGG - chr3_4:43371496-43371515 Msa0501800:intron 25.0%
! ATATGAAAATGTTAGGGTTT+GGG - chr3_4:43371497-43371516 Msa0501800:intron 25.0%
! TTTACTGTCATGCATTAAAT+TGG - chr3_4:43371525-43371544 Msa0501800:intron 25.0%
! TTACTGTCATGCATTAAATT+GGG - chr3_4:43371526-43371545 Msa0501800:intron 25.0%
! ATTCAGTTCAAACTAGTAAA+AGG + chr3_4:43372031-43372050 None:intergenic 25.0%
!!! TGCTTTGTTTATAATTACAC+AGG - chr3_4:43372054-43372073 Msa0501800:intron 25.0%
! TGTAAACTTGCAATCATTTA+AGG + chr3_4:43372189-43372208 None:intergenic 25.0%
! TCCATATAAAAACTAAACGT+TGG + chr3_4:43372221-43372240 None:intergenic 25.0%
!!! AACGTTTAGTTTTTATATGG+AGG - chr3_4:43372220-43372239 Msa0501800:intron 25.0%
!!! AAGATTGTGTTACTTAATGT+AGG + chr3_4:43370986-43371005 None:intergenic 25.0%
! TAATTCATTCATTTCTCTTC+TGG - chr3_4:43371016-43371035 Msa0501800:intron 25.0%
! TTAAGTTCATTCAGAATGTT+TGG - chr3_4:43371136-43371155 Msa0501800:intron 25.0%
! GTTATAAACTAAATTGTGTC+GGG + chr3_4:43371407-43371426 None:intergenic 25.0%
! AAAAAATTAGGGACTAATTC+GGG + chr3_4:43371441-43371460 None:intergenic 25.0%
! TTTATATTGTAGACAAGATC+AGG - chr3_4:43371458-43371477 Msa0501800:intron 25.0%
! TCCTCAATATGAAAATGTTA+GGG - chr3_4:43371491-43371510 Msa0501800:intron 25.0%
! AATATGAAAATGTTAGGGTT+TGG - chr3_4:43371496-43371515 Msa0501800:intron 25.0%
! ATATGAAAATGTTAGGGTTT+GGG - chr3_4:43371497-43371516 Msa0501800:intron 25.0%
! TTTACTGTCATGCATTAAAT+TGG - chr3_4:43371525-43371544 Msa0501800:intron 25.0%
! TTACTGTCATGCATTAAATT+GGG - chr3_4:43371526-43371545 Msa0501800:intron 25.0%
! ATTCAGTTCAAACTAGTAAA+AGG + chr3_4:43372031-43372050 None:intergenic 25.0%
!!! TGCTTTGTTTATAATTACAC+AGG - chr3_4:43372054-43372073 Msa0501800:intron 25.0%
! TGTAAACTTGCAATCATTTA+AGG + chr3_4:43372189-43372208 None:intergenic 25.0%
! TCCATATAAAAACTAAACGT+TGG + chr3_4:43372221-43372240 None:intergenic 25.0%
!!! AACGTTTAGTTTTTATATGG+AGG - chr3_4:43372220-43372239 Msa0501800:intron 25.0%
TCAAAATTTAAAGGGCAGTT+TGG - chr3_4:43370522-43370541 Msa0501800:three_prime_UTR 30.0%
! AGTTTGGTAATTGAAAAGAG+AGG - chr3_4:43370538-43370557 Msa0501800:three_prime_UTR 30.0%
! GTTTGGTAATTGAAAAGAGA+GGG - chr3_4:43370539-43370558 Msa0501800:three_prime_UTR 30.0%
GAACGAATGATGAATAGATT+CGG + chr3_4:43370667-43370686 None:intergenic 30.0%
AGTGAAAGAAGAAAGAACTT+GGG - chr3_4:43370693-43370712 Msa0501800:intron 30.0%
CATTAAGTAACACAATCTTC+AGG - chr3_4:43370987-43371006 Msa0501800:intron 30.0%
ATTCATTTCTCTTCTGGATA+AGG - chr3_4:43371022-43371041 Msa0501800:intron 30.0%
! CTTTTCATTATCTTATGAGG+AGG - chr3_4:43371201-43371220 Msa0501800:intron 30.0%
!! TATGAAGAATGGTGTTGTAT+TGG + chr3_4:43371337-43371356 None:intergenic 30.0%
!! TTTTTAGTGGAGATCCAATT+TGG - chr3_4:43371362-43371381 Msa0501800:intron 30.0%
AGAAAATTGTGAGACCAAAT+TGG + chr3_4:43371379-43371398 None:intergenic 30.0%
GTCCTCAATATGAAAATGTT+AGG - chr3_4:43371490-43371509 Msa0501800:intron 30.0%
! ACCCTAACATTTTCATATTG+AGG + chr3_4:43371495-43371514 None:intergenic 30.0%
GCAGCTTATATGATTGATAA+TGG - chr3_4:43371775-43371794 Msa0501800:intron 30.0%
ACATGGAACTTTAGATAAGA+TGG + chr3_4:43371835-43371854 None:intergenic 30.0%
GCAAGATAGCATTAGATTAA+TGG + chr3_4:43371978-43371997 None:intergenic 30.0%
!!! GCCAACGTTTAGTTTTTATA+TGG - chr3_4:43372217-43372236 Msa0501800:intron 30.0%
TCAAAATTTAAAGGGCAGTT+TGG - chr3_4:43370522-43370541 Msa0501800:three_prime_UTR 30.0%
! AGTTTGGTAATTGAAAAGAG+AGG - chr3_4:43370538-43370557 Msa0501800:three_prime_UTR 30.0%
! GTTTGGTAATTGAAAAGAGA+GGG - chr3_4:43370539-43370558 Msa0501800:three_prime_UTR 30.0%
GAACGAATGATGAATAGATT+CGG + chr3_4:43370667-43370686 None:intergenic 30.0%
AGTGAAAGAAGAAAGAACTT+GGG - chr3_4:43370693-43370712 Msa0501800:intron 30.0%
CATTAAGTAACACAATCTTC+AGG - chr3_4:43370987-43371006 Msa0501800:intron 30.0%
ATTCATTTCTCTTCTGGATA+AGG - chr3_4:43371022-43371041 Msa0501800:intron 30.0%
! CTTTTCATTATCTTATGAGG+AGG - chr3_4:43371201-43371220 Msa0501800:intron 30.0%
!! TATGAAGAATGGTGTTGTAT+TGG + chr3_4:43371337-43371356 None:intergenic 30.0%
!! TTTTTAGTGGAGATCCAATT+TGG - chr3_4:43371362-43371381 Msa0501800:intron 30.0%
AGAAAATTGTGAGACCAAAT+TGG + chr3_4:43371379-43371398 None:intergenic 30.0%
GTCCTCAATATGAAAATGTT+AGG - chr3_4:43371490-43371509 Msa0501800:intron 30.0%
! ACCCTAACATTTTCATATTG+AGG + chr3_4:43371495-43371514 None:intergenic 30.0%
GCAGCTTATATGATTGATAA+TGG - chr3_4:43371775-43371794 Msa0501800:intron 30.0%
ACATGGAACTTTAGATAAGA+TGG + chr3_4:43371835-43371854 None:intergenic 30.0%
GCAAGATAGCATTAGATTAA+TGG + chr3_4:43371978-43371997 None:intergenic 30.0%
!!! GCCAACGTTTAGTTTTTATA+TGG - chr3_4:43372217-43372236 Msa0501800:intron 30.0%
CTTAGTTAGTATCTCTCTCT+TGG + chr3_4:43370639-43370658 None:intergenic 35.0%
GAGTGAAAGAAGAAAGAACT+TGG - chr3_4:43370692-43370711 Msa0501800:intron 35.0%
GTGAAAGAAGAAAGAACTTG+GGG - chr3_4:43370694-43370713 Msa0501800:intron 35.0%
TGAAAGAAGAAAGAACTTGG+GGG - chr3_4:43370695-43370714 Msa0501800:intron 35.0%
TAACAACTGTTGTTCAGAGT+GGG + chr3_4:43370831-43370850 None:intergenic 35.0%
!!! ATCTCCGGTGAGAATTTTTT+GGG + chr3_4:43370904-43370923 None:intergenic 35.0%
CTGAATGAACTTAATCAACG+CGG + chr3_4:43371130-43371149 None:intergenic 35.0%
TCTTGATTGAATCCAACAAC+TGG - chr3_4:43371273-43371292 Msa0501800:intron 35.0%
AACTTAAGCCCTAATAAGCT+AGG - chr3_4:43371304-43371323 Msa0501800:intron 35.0%
AGTGTTTGTAGAACAAGTTG+AGG + chr3_4:43371651-43371670 None:intergenic 35.0%
ATAGCAGAGTTAGTAGTTCA+TGG + chr3_4:43371693-43371712 None:intergenic 35.0%
!! TTGATAATGGATGATGCAGA+TGG - chr3_4:43371788-43371807 Msa0501800:intron 35.0%
!! TGATAATGGATGATGCAGAT+GGG - chr3_4:43371789-43371808 Msa0501800:intron 35.0%
! ATGGGTTCTATCGTGAATTT+TGG - chr3_4:43371807-43371826 Msa0501800:intron 35.0%
TTGTTATGCTTGAGATTGCT+GGG - chr3_4:43372146-43372165 Msa0501800:intron 35.0%
GCAATCATTTAAGGAAGTGA+GGG + chr3_4:43372180-43372199 None:intergenic 35.0%
TGCAATCATTTAAGGAAGTG+AGG + chr3_4:43372181-43372200 None:intergenic 35.0%
! TTTATATGGAGGCCACATTT+TGG - chr3_4:43372231-43372250 Msa0501800:intron 35.0%
GTGTACGGAACTGTTATATT+TGG - chr3_4:43372253-43372272 Msa0501800:intron 35.0%
! AAGTATCGTGTCATACAACA+TGG - chr3_4:43372276-43372295 Msa0501800:intron 35.0%
CTTAGTTAGTATCTCTCTCT+TGG + chr3_4:43370639-43370658 None:intergenic 35.0%
GAGTGAAAGAAGAAAGAACT+TGG - chr3_4:43370692-43370711 Msa0501800:intron 35.0%
GTGAAAGAAGAAAGAACTTG+GGG - chr3_4:43370694-43370713 Msa0501800:intron 35.0%
TGAAAGAAGAAAGAACTTGG+GGG - chr3_4:43370695-43370714 Msa0501800:intron 35.0%
TAACAACTGTTGTTCAGAGT+GGG + chr3_4:43370831-43370850 None:intergenic 35.0%
!!! ATCTCCGGTGAGAATTTTTT+GGG + chr3_4:43370904-43370923 None:intergenic 35.0%
CTGAATGAACTTAATCAACG+CGG + chr3_4:43371130-43371149 None:intergenic 35.0%
TCTTGATTGAATCCAACAAC+TGG - chr3_4:43371273-43371292 Msa0501800:intron 35.0%
AACTTAAGCCCTAATAAGCT+AGG - chr3_4:43371304-43371323 Msa0501800:intron 35.0%
AGTGTTTGTAGAACAAGTTG+AGG + chr3_4:43371651-43371670 None:intergenic 35.0%
ATAGCAGAGTTAGTAGTTCA+TGG + chr3_4:43371693-43371712 None:intergenic 35.0%
!! TTGATAATGGATGATGCAGA+TGG - chr3_4:43371788-43371807 Msa0501800:intron 35.0%
!! TGATAATGGATGATGCAGAT+GGG - chr3_4:43371789-43371808 Msa0501800:intron 35.0%
! ATGGGTTCTATCGTGAATTT+TGG - chr3_4:43371807-43371826 Msa0501800:intron 35.0%
TTGTTATGCTTGAGATTGCT+GGG - chr3_4:43372146-43372165 Msa0501800:intron 35.0%
GCAATCATTTAAGGAAGTGA+GGG + chr3_4:43372180-43372199 None:intergenic 35.0%
TGCAATCATTTAAGGAAGTG+AGG + chr3_4:43372181-43372200 None:intergenic 35.0%
! TTTATATGGAGGCCACATTT+TGG - chr3_4:43372231-43372250 Msa0501800:intron 35.0%
GTGTACGGAACTGTTATATT+TGG - chr3_4:43372253-43372272 Msa0501800:intron 35.0%
! AAGTATCGTGTCATACAACA+TGG - chr3_4:43372276-43372295 Msa0501800:intron 35.0%
TGGTAATTGAAAAGAGAGGG+TGG - chr3_4:43370542-43370561 Msa0501800:three_prime_UTR 40.0%
GGTAATTGAAAAGAGAGGGT+GGG - chr3_4:43370543-43370562 Msa0501800:exon 40.0%
GTAATTGAAAAGAGAGGGTG+GGG - chr3_4:43370544-43370563 Msa0501800:exon 40.0%
TAATTGAAAAGAGAGGGTGG+GGG - chr3_4:43370545-43370564 Msa0501800:exon 40.0%
GGGATGAGAATATGGGATAA+CGG - chr3_4:43370565-43370584 Msa0501800:CDS 40.0%
! TGAGAATATGGGATAACGGT+TGG - chr3_4:43370569-43370588 Msa0501800:CDS 40.0%
AAAGAACTTGGGGGTTTCAA+TGG - chr3_4:43370704-43370723 Msa0501800:intron 40.0%
GTAACAACTGTTGTTCAGAG+TGG + chr3_4:43370832-43370851 None:intergenic 40.0%
GAAGCCCAAAAAATTCTCAC+CGG - chr3_4:43370897-43370916 Msa0501800:intron 40.0%
!!! CATCTCCGGTGAGAATTTTT+TGG + chr3_4:43370905-43370924 None:intergenic 40.0%
!! AGTTAAGAAGCACCAGTTGT+TGG + chr3_4:43371288-43371307 None:intergenic 40.0%
!! AGAATGGTGTTGTATTGGAG+TGG + chr3_4:43371332-43371351 None:intergenic 40.0%
TTAGGGACTAATTCGGGTAT+TGG + chr3_4:43371435-43371454 None:intergenic 40.0%
TGGGACAAATTGAAGAGTGA+TGG + chr3_4:43371596-43371615 None:intergenic 40.0%
CACTAGATGATTAACCTGTC+TGG - chr3_4:43371667-43371686 Msa0501800:intron 40.0%
GTTGTTATGCTTGAGATTGC+TGG - chr3_4:43372145-43372164 Msa0501800:intron 40.0%
TGGTAATTGAAAAGAGAGGG+TGG - chr3_4:43370542-43370561 Msa0501800:three_prime_UTR 40.0%
GGTAATTGAAAAGAGAGGGT+GGG - chr3_4:43370543-43370562 Msa0501800:exon 40.0%
GTAATTGAAAAGAGAGGGTG+GGG - chr3_4:43370544-43370563 Msa0501800:exon 40.0%
TAATTGAAAAGAGAGGGTGG+GGG - chr3_4:43370545-43370564 Msa0501800:exon 40.0%
GGGATGAGAATATGGGATAA+CGG - chr3_4:43370565-43370584 Msa0501800:CDS 40.0%
! TGAGAATATGGGATAACGGT+TGG - chr3_4:43370569-43370588 Msa0501800:CDS 40.0%
AAAGAACTTGGGGGTTTCAA+TGG - chr3_4:43370704-43370723 Msa0501800:intron 40.0%
GTAACAACTGTTGTTCAGAG+TGG + chr3_4:43370832-43370851 None:intergenic 40.0%
GAAGCCCAAAAAATTCTCAC+CGG - chr3_4:43370897-43370916 Msa0501800:intron 40.0%
!!! CATCTCCGGTGAGAATTTTT+TGG + chr3_4:43370905-43370924 None:intergenic 40.0%
!! AGTTAAGAAGCACCAGTTGT+TGG + chr3_4:43371288-43371307 None:intergenic 40.0%
!! AGAATGGTGTTGTATTGGAG+TGG + chr3_4:43371332-43371351 None:intergenic 40.0%
TTAGGGACTAATTCGGGTAT+TGG + chr3_4:43371435-43371454 None:intergenic 40.0%
TGGGACAAATTGAAGAGTGA+TGG + chr3_4:43371596-43371615 None:intergenic 40.0%
CACTAGATGATTAACCTGTC+TGG - chr3_4:43371667-43371686 Msa0501800:intron 40.0%
GTTGTTATGCTTGAGATTGC+TGG - chr3_4:43372145-43372164 Msa0501800:intron 40.0%
CAAACATCAGTCATACCTGC+TGG + chr3_4:43370467-43370486 None:intergenic 45.0%
! ACAAACCTTGGTGGTTGTTG+AGG + chr3_4:43370781-43370800 None:intergenic 45.0%
!! CTTGGGTTGGTGAAATCTCA+GGG + chr3_4:43370945-43370964 None:intergenic 45.0%
CCAAATGTGCTTGAGTGCTT+GGG + chr3_4:43370962-43370981 None:intergenic 45.0%
CCAAGCACTCAAGCACATTT+GGG - chr3_4:43370960-43370979 Msa0501800:intron 45.0%
GAGTGGCACCTAGCTTATTA+GGG + chr3_4:43371315-43371334 None:intergenic 45.0%
! AGTCCTTCACAGTTTTCTCG+TGG + chr3_4:43371552-43371571 None:intergenic 45.0%
TTAGTAGTTCATGGCCAGAC+AGG + chr3_4:43371684-43371703 None:intergenic 45.0%
GAATACGCCTCTTGGTTTCA+GGG + chr3_4:43372081-43372100 None:intergenic 45.0%
CTGTGATGAGAAGCTGAAGA+AGG - chr3_4:43372102-43372121 Msa0501800:intron 45.0%
AGCTGAAGAAGGTCTTTGCA+TGG - chr3_4:43372113-43372132 Msa0501800:intron 45.0%
GCTGAAGAAGGTCTTTGCAT+GGG - chr3_4:43372114-43372133 Msa0501800:intron 45.0%
CTGAAGAAGGTCTTTGCATG+GGG - chr3_4:43372115-43372134 Msa0501800:intron 45.0%
CAGTTCCGTACACCAAAATG+TGG + chr3_4:43372246-43372265 None:intergenic 45.0%
CAAACATCAGTCATACCTGC+TGG + chr3_4:43370467-43370486 None:intergenic 45.0%
! ACAAACCTTGGTGGTTGTTG+AGG + chr3_4:43370781-43370800 None:intergenic 45.0%
!! CTTGGGTTGGTGAAATCTCA+GGG + chr3_4:43370945-43370964 None:intergenic 45.0%
CCAAATGTGCTTGAGTGCTT+GGG + chr3_4:43370962-43370981 None:intergenic 45.0%
CCAAGCACTCAAGCACATTT+GGG - chr3_4:43370960-43370979 Msa0501800:intron 45.0%
GAGTGGCACCTAGCTTATTA+GGG + chr3_4:43371315-43371334 None:intergenic 45.0%
! AGTCCTTCACAGTTTTCTCG+TGG + chr3_4:43371552-43371571 None:intergenic 45.0%
TTAGTAGTTCATGGCCAGAC+AGG + chr3_4:43371684-43371703 None:intergenic 45.0%
GAATACGCCTCTTGGTTTCA+GGG + chr3_4:43372081-43372100 None:intergenic 45.0%
CTGTGATGAGAAGCTGAAGA+AGG - chr3_4:43372102-43372121 Msa0501800:intron 45.0%
AGCTGAAGAAGGTCTTTGCA+TGG - chr3_4:43372113-43372132 Msa0501800:intron 45.0%
GCTGAAGAAGGTCTTTGCAT+GGG - chr3_4:43372114-43372133 Msa0501800:intron 45.0%
CTGAAGAAGGTCTTTGCATG+GGG - chr3_4:43372115-43372134 Msa0501800:intron 45.0%
CAGTTCCGTACACCAAAATG+TGG + chr3_4:43372246-43372265 None:intergenic 45.0%
!!! TAGTTTTTTTTTTTTTTTTT+TGG + chr3_4:43371061-43371080 None:intergenic 5.0%
!!! TAGTTTTTTTTTTTTTTTTT+TGG + chr3_4:43371061-43371080 None:intergenic 5.0%
AGGGTGGGGGATGAGAATAT+GGG - chr3_4:43370558-43370577 Msa0501800:exon 50.0%
! GATAACGGTTGGAGCAGAGT+GGG - chr3_4:43370580-43370599 Msa0501800:CDS 50.0%
ATAACGGTTGGAGCAGAGTG+GGG - chr3_4:43370581-43370600 Msa0501800:CDS 50.0%
TCATCCCCACTCTAATCCAC+AGG - chr3_4:43370755-43370774 Msa0501800:intron 50.0%
!! TGAGGCCTGTGGATTAGAGT+GGG + chr3_4:43370763-43370782 None:intergenic 50.0%
!! TTGAGGCCTGTGGATTAGAG+TGG + chr3_4:43370764-43370783 None:intergenic 50.0%
GGAAACGCAACAAACCTTGG+TGG + chr3_4:43370790-43370809 None:intergenic 50.0%
GTGGGAAACGCAACAAACCT+TGG + chr3_4:43370793-43370812 None:intergenic 50.0%
!! GCTTGGGTTGGTGAAATCTC+AGG + chr3_4:43370946-43370965 None:intergenic 50.0%
ATGTGCTTGAGTGCTTGGGT+TGG + chr3_4:43370958-43370977 None:intergenic 50.0%
CCCAAGCACTCAAGCACATT+TGG - chr3_4:43370959-43370978 Msa0501800:intron 50.0%
CCCAAATGTGCTTGAGTGCT+TGG + chr3_4:43370963-43370982 None:intergenic 50.0%
GGAGTGGCACCTAGCTTATT+AGG + chr3_4:43371316-43371335 None:intergenic 50.0%
CGAATACGCCTCTTGGTTTC+AGG + chr3_4:43372082-43372101 None:intergenic 50.0%
ATCACAGCGAATACGCCTCT+TGG + chr3_4:43372089-43372108 None:intergenic 50.0%
!! GGAGGCCACATTTTGGTGTA+CGG - chr3_4:43372238-43372257 Msa0501800:intron 50.0%
AGGGTGGGGGATGAGAATAT+GGG - chr3_4:43370558-43370577 Msa0501800:exon 50.0%
! GATAACGGTTGGAGCAGAGT+GGG - chr3_4:43370580-43370599 Msa0501800:CDS 50.0%
ATAACGGTTGGAGCAGAGTG+GGG - chr3_4:43370581-43370600 Msa0501800:CDS 50.0%
TCATCCCCACTCTAATCCAC+AGG - chr3_4:43370755-43370774 Msa0501800:intron 50.0%
!! TGAGGCCTGTGGATTAGAGT+GGG + chr3_4:43370763-43370782 None:intergenic 50.0%
!! TTGAGGCCTGTGGATTAGAG+TGG + chr3_4:43370764-43370783 None:intergenic 50.0%
GGAAACGCAACAAACCTTGG+TGG + chr3_4:43370790-43370809 None:intergenic 50.0%
GTGGGAAACGCAACAAACCT+TGG + chr3_4:43370793-43370812 None:intergenic 50.0%
!! GCTTGGGTTGGTGAAATCTC+AGG + chr3_4:43370946-43370965 None:intergenic 50.0%
ATGTGCTTGAGTGCTTGGGT+TGG + chr3_4:43370958-43370977 None:intergenic 50.0%
CCCAAGCACTCAAGCACATT+TGG - chr3_4:43370959-43370978 Msa0501800:intron 50.0%
CCCAAATGTGCTTGAGTGCT+TGG + chr3_4:43370963-43370982 None:intergenic 50.0%
GGAGTGGCACCTAGCTTATT+AGG + chr3_4:43371316-43371335 None:intergenic 50.0%
CGAATACGCCTCTTGGTTTC+AGG + chr3_4:43372082-43372101 None:intergenic 50.0%
ATCACAGCGAATACGCCTCT+TGG + chr3_4:43372089-43372108 None:intergenic 50.0%
!! GGAGGCCACATTTTGGTGTA+CGG - chr3_4:43372238-43372257 Msa0501800:intron 50.0%
GAGGGTGGGGGATGAGAATA+TGG - chr3_4:43370557-43370576 Msa0501800:exon 55.0%
! GGATAACGGTTGGAGCAGAG+TGG - chr3_4:43370579-43370598 Msa0501800:CDS 55.0%
!! GAGGCCTGTGGATTAGAGTG+GGG + chr3_4:43370762-43370781 None:intergenic 55.0%
! TTGGTGGTTGTTGAGGCCTG+TGG + chr3_4:43370774-43370793 None:intergenic 55.0%
ACAGGCCTCAACAACCACCA+AGG - chr3_4:43370773-43370792 Msa0501800:intron 55.0%
GGGTTGCATCTCGTAGGTGT+GGG + chr3_4:43370811-43370830 None:intergenic 55.0%
TGGGTTGCATCTCGTAGGTG+TGG + chr3_4:43370812-43370831 None:intergenic 55.0%
!! CAGAGTGGGTTGCATCTCGT+AGG + chr3_4:43370817-43370836 None:intergenic 55.0%
AAATCTCAGGGAGACCGACG+AGG + chr3_4:43370933-43370952 None:intergenic 55.0%
GGGCCACGAGAAAACTGTGA+AGG - chr3_4:43371546-43371565 Msa0501800:intron 55.0%
CACAGGACCCTGAAACCAAG+AGG - chr3_4:43372071-43372090 Msa0501800:intron 55.0%
GAGGGTGGGGGATGAGAATA+TGG - chr3_4:43370557-43370576 Msa0501800:exon 55.0%
! GGATAACGGTTGGAGCAGAG+TGG - chr3_4:43370579-43370598 Msa0501800:CDS 55.0%
!! GAGGCCTGTGGATTAGAGTG+GGG + chr3_4:43370762-43370781 None:intergenic 55.0%
! TTGGTGGTTGTTGAGGCCTG+TGG + chr3_4:43370774-43370793 None:intergenic 55.0%
ACAGGCCTCAACAACCACCA+AGG - chr3_4:43370773-43370792 Msa0501800:intron 55.0%
GGGTTGCATCTCGTAGGTGT+GGG + chr3_4:43370811-43370830 None:intergenic 55.0%
TGGGTTGCATCTCGTAGGTG+TGG + chr3_4:43370812-43370831 None:intergenic 55.0%
!! CAGAGTGGGTTGCATCTCGT+AGG + chr3_4:43370817-43370836 None:intergenic 55.0%
AAATCTCAGGGAGACCGACG+AGG + chr3_4:43370933-43370952 None:intergenic 55.0%
GGGCCACGAGAAAACTGTGA+AGG - chr3_4:43371546-43371565 Msa0501800:intron 55.0%
CACAGGACCCTGAAACCAAG+AGG - chr3_4:43372071-43372090 Msa0501800:intron 55.0%
TCTGCCACTGTCTCTCCCAG+CGG - chr3_4:43370853-43370872 Msa0501800:intron 60.0%
CCGACGAGGTCTTGCATCTC+CGG + chr3_4:43370919-43370938 None:intergenic 60.0%
CCGGAGATGCAAGACCTCGT+CGG - chr3_4:43370916-43370935 Msa0501800:intron 60.0%
TCTGCCACTGTCTCTCCCAG+CGG - chr3_4:43370853-43370872 Msa0501800:intron 60.0%
CCGACGAGGTCTTGCATCTC+CGG + chr3_4:43370919-43370938 None:intergenic 60.0%
CCGGAGATGCAAGACCTCGT+CGG - chr3_4:43370916-43370935 Msa0501800:intron 60.0%
! CCGCGAATTTTCCCGCCGCT+GGG + chr3_4:43370871-43370890 None:intergenic 65.0%
CCCAGCGGCGGGAAAATTCG+CGG - chr3_4:43370868-43370887 Msa0501800:intron 65.0%
! ACCGCGAATTTTCCCGCCGC+TGG + chr3_4:43370872-43370891 None:intergenic 65.0%
! CCGCGAATTTTCCCGCCGCT+GGG + chr3_4:43370871-43370890 None:intergenic 65.0%
CCCAGCGGCGGGAAAATTCG+CGG - chr3_4:43370868-43370887 Msa0501800:intron 65.0%
! ACCGCGAATTTTCCCGCCGC+TGG + chr3_4:43370872-43370891 None:intergenic 65.0%
GCCACTGTCTCTCCCAGCGG+CGG - chr3_4:43370856-43370875 Msa0501800:intron 70.0%
CCACTGTCTCTCCCAGCGGC+GGG - chr3_4:43370857-43370876 Msa0501800:intron 70.0%
GCCACTGTCTCTCCCAGCGG+CGG - chr3_4:43370856-43370875 Msa0501800:intron 70.0%
CCACTGTCTCTCCCAGCGGC+GGG - chr3_4:43370857-43370876 Msa0501800:intron 70.0%
CCCGCCGCTGGGAGAGACAG+TGG + chr3_4:43370860-43370879 None:intergenic 75.0%
CCCGCCGCTGGGAGAGACAG+TGG + chr3_4:43370860-43370879 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 43370458 43372299 43370458 ID=Msa0501800;Name=Msa0501800
chr3_4 mRNA 43370458 43372299 43370458 ID=Msa0501800-mRNA-1;Parent=Msa0501800;Name=Msa0501800-mRNA-1;_AED=0.47;_eAED=0.65;_QI=0|0|0.33|0.66|0.5|0.66|3|104|81
chr3_4 exon 43370458 43370681 43370458 ID=Msa0501800-mRNA-1:exon:9232;Parent=Msa0501800-mRNA-1
chr3_4 exon 43371868 43371980 43371868 ID=Msa0501800-mRNA-1:exon:9231;Parent=Msa0501800-mRNA-1
chr3_4 exon 43372287 43372299 43372287 ID=Msa0501800-mRNA-1:exon:9230;Parent=Msa0501800-mRNA-1
chr3_4 CDS 43372287 43372299 43372287 ID=Msa0501800-mRNA-1:cds;Parent=Msa0501800-mRNA-1
chr3_4 CDS 43371868 43371980 43371868 ID=Msa0501800-mRNA-1:cds;Parent=Msa0501800-mRNA-1
chr3_4 CDS 43370562 43370681 43370562 ID=Msa0501800-mRNA-1:cds;Parent=Msa0501800-mRNA-1
chr3_4 three_prime_UTR 43370458 43370561 43370458 ID=Msa0501800-mRNA-1:three_prime_utr;Parent=Msa0501800-mRNA-1
Gene Sequence

>Msa0501800

ATGATACCAGCAGGCCTCAACAACCACCAAGGTTTGTTGCGTTTCCCACACCTACGAGATGCAACCCACTCTGAACAACAGTTGTTACTTCTGCCACTGTCTCTCCCAGCGGCGGGAAAATTCGCGGACCCTGAAACCAAGAGGCGTATTCGCTGTGATGAGAAGCTGAAGAAGGTCTTTGCATGGGGAGATGAAGTTGTTATGCTTGAGATTGCTGGGTTGATAAGCCCTCACTTCCTTAAATGA
Protein sequence

>Msa0501800

MIPAGLNNHQGLLRFPHLRDATHSEQQLLLLPLSLPAAGKFADPETKRRIRCDEKLKKVFAWGDEVVMLEIAGLISPHFLK*