AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0054730


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054730 MtrunA17_Chr1g0150111 96.471 170 6 0 1 170 1 170 1.21e-110 326
Msa0054730 MtrunA17_Chr5g0410321 46.809 188 60 3 1 188 62 209 2.43e-37 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054730 AT4G31180.1 41.398 186 91 4 3 170 2 187 7.79e-29 112
Msa0054730 AT4G31180.2 41.398 186 91 4 3 170 2 187 7.79e-29 112
Msa0054730 AT4G26870.1 42.262 168 90 5 28 194 23 184 6.15e-25 101

Find 59 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCAGCCGCTCCTATTAA+AGG 0.200895 1_2:-9445741 Msa0054730:CDS
CTTTGCGGCATCCTTCTTGC+AGG 0.276045 1_2:+9446145 None:intergenic
TTCTTAGTCATCAGAGAAAA+TGG 0.279492 1_2:-9445879 Msa0054730:CDS
GATCCCCTTGCTGTCAATTA+TGG 0.290000 1_2:-9446053 Msa0054730:CDS
TGTTGTGAGGCACCTTTAAT+AGG 0.308209 1_2:+9445729 None:intergenic
CACCGTACAGTGCTTGGTTC+AGG 0.346864 1_2:-9445853 Msa0054730:CDS
GTCCAGTCGTTGACATTGAC+AGG 0.393463 1_2:+9445996 None:intergenic
AGGTGGCGGCGGCAACTTCT+CGG 0.397158 1_2:+9446105 None:intergenic
GTTATGATATCTTGTTACTT+AGG 0.401643 1_2:-9445701 Msa0054730:CDS
CAAGAAGGATGCCGCAAAGC+TGG 0.416596 1_2:-9446141 Msa0054730:CDS
CATTGACAGGGGTCTTCGAT+TGG 0.443151 1_2:+9446009 None:intergenic
ATCTGCGGACTCACTAAATC+AGG 0.450583 1_2:+9445825 None:intergenic
AACGCCATCTTCTTCCCAAC+TGG 0.452718 1_2:+9445900 None:intergenic
GCCCTCCCACGGATTAGAAC+CGG 0.456675 1_2:+9445936 None:intergenic
TATTAAAGGTGCCTCACAAC+AGG 0.473545 1_2:-9445727 Msa0054730:CDS
TTTGTGAGTTGTGATTGTTT+AGG 0.489001 1_2:-9445645 Msa0054730:CDS
TGAGGCTCTCAATGATTCAC+TGG 0.499024 1_2:-9445967 Msa0054730:CDS
TGGTTTCACCGTACAGTGCT+TGG 0.499781 1_2:-9445859 Msa0054730:CDS
TTTAATAGGAGCGGCTGGAA+TGG 0.513685 1_2:+9445743 None:intergenic
GAGTCTATTGTCGATGTCGA+AGG 0.513770 1_2:-9445774 Msa0054730:CDS
GCACCTTTAATAGGAGCGGC+TGG 0.516328 1_2:+9445738 None:intergenic
CACAAGCTATTCGACCAGTT+GGG 0.517268 1_2:-9445914 Msa0054730:CDS
GCACAAGCTATTCGACCAGT+TGG 0.517849 1_2:-9445915 Msa0054730:CDS
GAATAGCTTGTGCCCTCCCA+CGG 0.535699 1_2:+9445925 None:intergenic
CGATGTAGCAGAGGTGGCGG+CGG 0.543849 1_2:+9446094 None:intergenic
AACAAGCCGGTTCTAATCCG+TGG 0.547533 1_2:-9445942 Msa0054730:CDS
CCCCTGTCAATGTCAACGAC+TGG 0.551263 1_2:-9445998 Msa0054730:CDS
TCGATTGGAGCTCAATGAGC+GGG 0.557785 1_2:+9446024 None:intergenic
AGTCGATGTAGCAGAGGTGG+CGG 0.572655 1_2:+9446091 None:intergenic
TCGACCAGTTGGGAAGAAGA+TGG 0.581394 1_2:-9445904 Msa0054730:CDS
AGACTCACGGCTGAGAGCGG+CGG 0.581736 1_2:+9445791 None:intergenic
AATCATTGAGAGCCTCAACT+CGG 0.583678 1_2:+9445973 None:intergenic
GAGAGTCGATGTAGCAGAGG+TGG 0.585897 1_2:+9446088 None:intergenic
TCCAGTCGTTGACATTGACA+GGG 0.592334 1_2:+9445997 None:intergenic
AGAGAGAGTCGATGTAGCAG+AGG 0.593608 1_2:+9446085 None:intergenic
TGATTTAGTGAGTCCGCAGA+TGG 0.596877 1_2:-9445823 Msa0054730:CDS
ATCATTGAGAGCCTCAACTC+GGG 0.599803 1_2:+9445974 None:intergenic
CAACGACTGGACCCGAGTTG+AGG 0.600329 1_2:-9445985 Msa0054730:CDS
TCAAGAGCCGACACTGTCAT+CGG 0.603493 1_2:-9446195 Msa0054730:CDS
GATCCTTCCGATGACAGTGT+CGG 0.605021 1_2:+9446188 None:intergenic
TGAGGCACCTTTAATAGGAG+CGG 0.608618 1_2:+9445734 None:intergenic
GCCGGTTCTAATCCGTGGGA+GGG 0.610769 1_2:-9445937 Msa0054730:CDS
TTCGATTGGAGCTCAATGAG+CGG 0.611035 1_2:+9446023 None:intergenic
TGATTCACTGGAGAACAAGC+CGG 0.613416 1_2:-9445955 Msa0054730:CDS
GCGGCAAATTTCACCATCTG+CGG 0.614321 1_2:+9445810 None:intergenic
ATACCATAATTGACAGCAAG+GGG 0.614733 1_2:+9446050 None:intergenic
ACAAGCCGGTTCTAATCCGT+GGG 0.615482 1_2:-9445941 Msa0054730:CDS
TATTGTCGATGTCGAAGGTG+TGG 0.616371 1_2:-9445769 Msa0054730:CDS
AATAGACTCACGGCTGAGAG+CGG 0.623488 1_2:+9445788 None:intergenic
AGCCGGTTCTAATCCGTGGG+AGG 0.623958 1_2:-9445938 Msa0054730:CDS
GAGCCGACACTGTCATCGGA+AGG 0.625324 1_2:-9446191 Msa0054730:CDS
CAATACCATAATTGACAGCA+AGG 0.634292 1_2:+9446048 None:intergenic
TGCCTGAACCAAGCACTGTA+CGG 0.635520 1_2:+9445851 None:intergenic
ATCATAACCAACCTGTTGTG+AGG 0.639858 1_2:+9445716 None:intergenic
AAAGGTGCCTCACAACAGGT+TGG 0.643312 1_2:-9445723 Msa0054730:CDS
CGACATCGACAATAGACTCA+CGG 0.651944 1_2:+9445778 None:intergenic
AATACCATAATTGACAGCAA+GGG 0.690003 1_2:+9446049 None:intergenic
TGGCGGCGGCAACTTCTCGG+CGG 0.706873 1_2:+9446108 None:intergenic
CCAGTCGTTGACATTGACAG+GGG 0.731951 1_2:+9445998 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCAGAAACAAAAAGCAAAA+AGG - chr1_2:9445682-9445701 Msa0054730:CDS 25.0%
!! GTTATGATATCTTGTTACTT+AGG - chr1_2:9446149-9446168 Msa0054730:CDS 25.0%
AATACCATAATTGACAGCAA+GGG + chr1_2:9445804-9445823 None:intergenic 30.0%
TTCTTAGTCATCAGAGAAAA+TGG - chr1_2:9445971-9445990 Msa0054730:CDS 30.0%
TTTGTGAGTTGTGATTGTTT+AGG - chr1_2:9446205-9446224 Msa0054730:CDS 30.0%
ATACCATAATTGACAGCAAG+GGG + chr1_2:9445803-9445822 None:intergenic 35.0%
CAATACCATAATTGACAGCA+AGG + chr1_2:9445805-9445824 None:intergenic 35.0%
AATCATTGAGAGCCTCAACT+CGG + chr1_2:9445880-9445899 None:intergenic 40.0%
! TGTTGTGAGGCACCTTTAAT+AGG + chr1_2:9446124-9446143 None:intergenic 40.0%
!! TATTAAAGGTGCCTCACAAC+AGG - chr1_2:9446123-9446142 Msa0054730:CDS 40.0%
ATCATAACCAACCTGTTGTG+AGG + chr1_2:9446137-9446156 None:intergenic 40.0%
AAGCAAAAAGGCCTGCAAGA+AGG - chr1_2:9445694-9445713 Msa0054730:CDS 45.0%
! GATCCCCTTGCTGTCAATTA+TGG - chr1_2:9445797-9445816 Msa0054730:CDS 45.0%
TTCGATTGGAGCTCAATGAG+CGG + chr1_2:9445830-9445849 None:intergenic 45.0%
TCCAGTCGTTGACATTGACA+GGG + chr1_2:9445856-9445875 None:intergenic 45.0%
ATCATTGAGAGCCTCAACTC+GGG + chr1_2:9445879-9445898 None:intergenic 45.0%
TGAGGCTCTCAATGATTCAC+TGG - chr1_2:9445883-9445902 Msa0054730:CDS 45.0%
TGATTCACTGGAGAACAAGC+CGG - chr1_2:9445895-9445914 Msa0054730:CDS 45.0%
CACAAGCTATTCGACCAGTT+GGG - chr1_2:9445936-9445955 Msa0054730:CDS 45.0%
ATCTGCGGACTCACTAAATC+AGG + chr1_2:9446028-9446047 None:intergenic 45.0%
TGATTTAGTGAGTCCGCAGA+TGG - chr1_2:9446027-9446046 Msa0054730:CDS 45.0%
CGACATCGACAATAGACTCA+CGG + chr1_2:9446075-9446094 None:intergenic 45.0%
GAGTCTATTGTCGATGTCGA+AGG - chr1_2:9446076-9446095 Msa0054730:CDS 45.0%
TATTGTCGATGTCGAAGGTG+TGG - chr1_2:9446081-9446100 Msa0054730:CDS 45.0%
TTTAATAGGAGCGGCTGGAA+TGG + chr1_2:9446110-9446129 None:intergenic 45.0%
ATTCCAGCCGCTCCTATTAA+AGG - chr1_2:9446109-9446128 Msa0054730:CDS 45.0%
! TGAGGCACCTTTAATAGGAG+CGG + chr1_2:9446119-9446138 None:intergenic 45.0%
TCAAGAGCCGACACTGTCAT+CGG - chr1_2:9445655-9445674 Msa0054730:CDS 50.0%
GATCCTTCCGATGACAGTGT+CGG + chr1_2:9445665-9445684 None:intergenic 50.0%
!!! GCGTTGCTTTTCCAGCTTTG+CGG + chr1_2:9445723-9445742 None:intergenic 50.0%
AGAGAGAGTCGATGTAGCAG+AGG + chr1_2:9445768-9445787 None:intergenic 50.0%
TCGATTGGAGCTCAATGAGC+GGG + chr1_2:9445829-9445848 None:intergenic 50.0%
CATTGACAGGGGTCTTCGAT+TGG + chr1_2:9445844-9445863 None:intergenic 50.0%
CCAGTCGTTGACATTGACAG+GGG + chr1_2:9445855-9445874 None:intergenic 50.0%
GTCCAGTCGTTGACATTGAC+AGG + chr1_2:9445857-9445876 None:intergenic 50.0%
! AACAAGCCGGTTCTAATCCG+TGG - chr1_2:9445908-9445927 Msa0054730:CDS 50.0%
! ACAAGCCGGTTCTAATCCGT+GGG - chr1_2:9445909-9445928 Msa0054730:CDS 50.0%
GCACAAGCTATTCGACCAGT+TGG - chr1_2:9445935-9445954 Msa0054730:CDS 50.0%
TCGACCAGTTGGGAAGAAGA+TGG - chr1_2:9445946-9445965 Msa0054730:CDS 50.0%
AACGCCATCTTCTTCCCAAC+TGG + chr1_2:9445953-9445972 None:intergenic 50.0%
TGGTTTCACCGTACAGTGCT+TGG - chr1_2:9445991-9446010 Msa0054730:CDS 50.0%
! TGCCTGAACCAAGCACTGTA+CGG + chr1_2:9446002-9446021 None:intergenic 50.0%
GCGGCAAATTTCACCATCTG+CGG + chr1_2:9446043-9446062 None:intergenic 50.0%
AATAGACTCACGGCTGAGAG+CGG + chr1_2:9446065-9446084 None:intergenic 50.0%
!! AAAGGTGCCTCACAACAGGT+TGG - chr1_2:9446127-9446146 Msa0054730:CDS 50.0%
CTTTGCGGCATCCTTCTTGC+AGG + chr1_2:9445708-9445727 None:intergenic 55.0%
CAAGAAGGATGCCGCAAAGC+TGG - chr1_2:9445709-9445728 Msa0054730:CDS 55.0%
AGTCGATGTAGCAGAGGTGG+CGG + chr1_2:9445762-9445781 None:intergenic 55.0%
GAGAGTCGATGTAGCAGAGG+TGG + chr1_2:9445765-9445784 None:intergenic 55.0%
CCCCTGTCAATGTCAACGAC+TGG - chr1_2:9445852-9445871 Msa0054730:CDS 55.0%
! GAATAGCTTGTGCCCTCCCA+CGG + chr1_2:9445928-9445947 None:intergenic 55.0%
CACCGTACAGTGCTTGGTTC+AGG - chr1_2:9445997-9446016 Msa0054730:CDS 55.0%
GCACCTTTAATAGGAGCGGC+TGG + chr1_2:9446115-9446134 None:intergenic 55.0%
GAGCCGACACTGTCATCGGA+AGG - chr1_2:9445659-9445678 Msa0054730:CDS 60.0%
CAACGACTGGACCCGAGTTG+AGG - chr1_2:9445865-9445884 Msa0054730:CDS 60.0%
! AGCCGGTTCTAATCCGTGGG+AGG - chr1_2:9445912-9445931 Msa0054730:CDS 60.0%
! GCCGGTTCTAATCCGTGGGA+GGG - chr1_2:9445913-9445932 Msa0054730:CDS 60.0%
!! GCCCTCCCACGGATTAGAAC+CGG + chr1_2:9445917-9445936 None:intergenic 60.0%
! AGGTGGCGGCGGCAACTTCT+CGG + chr1_2:9445748-9445767 None:intergenic 65.0%
! CGATGTAGCAGAGGTGGCGG+CGG + chr1_2:9445759-9445778 None:intergenic 65.0%
AGACTCACGGCTGAGAGCGG+CGG + chr1_2:9446062-9446081 None:intergenic 65.0%
TGGCGGCGGCAACTTCTCGG+CGG + chr1_2:9445745-9445764 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 9445638 9446234 9445638 ID=Msa0054730;Name=Msa0054730
chr1_2 mRNA 9445638 9446234 9445638 ID=Msa0054730-mRNA-1;Parent=Msa0054730;Name=Msa0054730-mRNA-1;_AED=0.53;_eAED=0.53;_QI=0|-1|0|1|-1|1|1|0|198
chr1_2 exon 9445638 9446234 9445638 ID=Msa0054730-mRNA-1:exon:3516;Parent=Msa0054730-mRNA-1
chr1_2 CDS 9445638 9446234 9445638 ID=Msa0054730-mRNA-1:cds;Parent=Msa0054730-mRNA-1
Gene Sequence

>Msa0054730

ATGTCAACTTCAGAATCTCAAGAGCCGACACTGTCATCGGAAGGATCAGAAACAAAAAGCAAAAAGGCCTGCAAGAAGGATGCCGCAAAGCTGGAAAAGCAACGCCGCCGAGAAGTTGCCGCCGCCACCTCTGCTACATCGACTCTCTCTGTCGAAGATGATCCCCTTGCTGTCAATTATGGTATTGTCCCGCTCATTGAGCTCCAATCGAAGACCCCTGTCAATGTCAACGACTGGACCCGAGTTGAGGCTCTCAATGATTCACTGGAGAACAAGCCGGTTCTAATCCGTGGGAGGGCACAAGCTATTCGACCAGTTGGGAAGAAGATGGCGTTCTTAGTCATCAGAGAAAATGGTTTCACCGTACAGTGCTTGGTTCAGGCACAACCTGATTTAGTGAGTCCGCAGATGGTGAAATTTGCCGCCGCTCTCAGCCGTGAGTCTATTGTCGATGTCGAAGGTGTGGTTTCCATTCCAGCCGCTCCTATTAAAGGTGCCTCACAACAGGTTGGTTATGATATCTTGTTACTTAGGTTTTGTGATTTTGTCAGTGTTTTGAATTTAGAGTTTGTGAGTTGTGATTGTTTAGGATTTTGA

Protein sequence

>Msa0054730

MSTSESQEPTLSSEGSETKSKKACKKDAAKLEKQRRREVAAATSATSTLSVEDDPLAVNYGIVPLIELQSKTPVNVNDWTRVEALNDSLENKPVLIRGRAQAIRPVGKKMAFLVIRENGFTVQCLVQAQPDLVSPQMVKFAAALSRESIVDVEGVVSIPAAPIKGASQQVGYDILLLRFCDFVSVLNLEFVSCDCLGF*