AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0502800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0502800 MtrunA17_Chr3g0101311 85.042 361 34 2 1 341 100 460 0.0 600
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0502800 AT3G20170.1 48.919 370 159 5 1 343 102 468 2.31e-104 315

Find 89 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAAGGAGATGATGTTTC+TGG 0.181044 3_4:-45501965 Msa0502800:CDS
ATCATGTTTAATTATCTTAC+AGG 0.218306 3_4:-45500292 Msa0502800:exon
CAGCAGCTGTCGATGTGATA+TGG 0.278624 3_4:-45501829 Msa0502800:CDS
TGGTGATGCAAGAGCAATTC+CGG 0.352200 3_4:-45501648 Msa0502800:CDS
GGACCATTCTACCACTTTCC+TGG 0.363654 3_4:+45501672 None:intergenic
TTACAACATACATCTATGAT+AGG 0.385514 3_4:+45500202 None:intergenic
CGAGTGTTGAGCAACTCATT+TGG 0.385896 3_4:-45500322 Msa0502800:CDS
TGGCGCTTCAGATGGAAGTT+AGG 0.404983 3_4:-45502249 Msa0502800:CDS
GATTTACTACATGACGAGAC+TGG 0.405844 3_4:-45501617 Msa0502800:CDS
ATAGCCCCAGCTTGAGCAAT+TGG 0.406485 3_4:+45502007 None:intergenic
GTATATAGTCATTTGCTTGT+AGG 0.407308 3_4:-45500123 Msa0502800:three_prime_UTR
ATGTATGTTGTAATGAAAAT+TGG 0.409597 3_4:-45500192 Msa0502800:three_prime_UTR
TAAAAGCCGCACCTGAGTTC+CGG 0.410207 3_4:+45501771 None:intergenic
AGGAGGGAGCTCATTAGATT+AGG 0.412597 3_4:-45502229 Msa0502800:CDS
TAATTATAAGTTACAACAAT+AGG 0.413182 3_4:+45500062 None:intergenic
GTTGTATAATTTATAAAAGA+GGG 0.413839 3_4:-45500147 Msa0502800:three_prime_UTR
TCTTTATTTCTTCAGTCCCA+TGG 0.430621 3_4:+45501729 None:intergenic
TAGCACACCAAGAGTATTAC+CGG 0.430738 3_4:+45502054 None:intergenic
ATCAGACCAATTGCTCAAGC+TGG 0.432583 3_4:-45502013 Msa0502800:CDS
CTTTATGCGGAGCTTCTTCA+AGG 0.433825 3_4:-45501980 Msa0502800:CDS
TGTTGAAATTGTGGGTCATT+TGG 0.446283 3_4:-45501888 Msa0502800:CDS
GTGTTGCACGTTCTTTGTTA+AGG 0.459610 3_4:-45502456 Msa0502800:CDS
TGTTGTATAATTTATAAAAG+AGG 0.462397 3_4:-45500148 Msa0502800:three_prime_UTR
AGTGTTGATAGAATGTTTGT+TGG 0.463303 3_4:-45502401 Msa0502800:CDS
TGAGCAACTCATTTGGATCC+TGG 0.466205 3_4:-45500315 Msa0502800:CDS
TGTAGTAAATCAATAAGAAC+CGG 0.466912 3_4:+45501629 None:intergenic
CTTTGAACCTCGATTAAACC+TGG 0.467265 3_4:+45499985 None:intergenic
AGCAGCTGTCGATGTGATAT+GGG 0.479152 3_4:-45501828 Msa0502800:CDS
CTTGTTGAGTTGTTGAGCCA+TGG 0.489304 3_4:-45501746 Msa0502800:CDS
AGGAGATGATGTTTCTGGTA+AGG 0.494027 3_4:-45501960 Msa0502800:CDS
CGCTTCAGATGGAAGTTAGG+AGG 0.496594 3_4:-45502246 Msa0502800:CDS
ATTGTTGAGGCTGCTGGTGT+TGG 0.498526 3_4:-45502193 Msa0502800:CDS
AGCACACACCTTCTGTGATC+CGG 0.498591 3_4:-45501790 Msa0502800:CDS
ATTTACTACATGACGAGACT+GGG 0.505397 3_4:-45501616 Msa0502800:CDS
AAGGTTGTTGCCTCGTGCCG+AGG 0.506613 3_4:-45502437 Msa0502800:CDS
TTCGTATTCATGCATCGAAT+GGG 0.509754 3_4:-45500370 Msa0502800:CDS
TAACATGATTTCGAGGGAAA+GGG 0.510380 3_4:-45502170 Msa0502800:CDS
ACTAAGAAATAATGTGGTTG+AGG 0.515775 3_4:-45501591 Msa0502800:CDS
TGAAGAAGCTCCGCATAAAG+AGG 0.516045 3_4:+45501983 None:intergenic
TCAGACCAATTGCTCAAGCT+GGG 0.516253 3_4:-45502012 Msa0502800:CDS
TTGTTGAGTTGTTGAGCCAT+GGG 0.519539 3_4:-45501745 Msa0502800:CDS
GAGTGCGTTGGCGCTTCAGA+TGG 0.520748 3_4:-45502257 Msa0502800:CDS
AAGATAATTAAACATGATCC+AGG 0.523474 3_4:+45500297 None:intergenic
CTTGTGGAGTTGTTTAGTGA+TGG 0.523872 3_4:-45502100 Msa0502800:CDS
GTAACATGATTTCGAGGGAA+AGG 0.527617 3_4:-45502171 Msa0502800:CDS
GAAAGGGCGTGTCAATCGAT+TGG 0.529311 3_4:-45502154 Msa0502800:CDS
ATTCGTATTCATGCATCGAA+TGG 0.531293 3_4:-45500371 Msa0502800:CDS
TGTCAATCGATTGGATTGGT+TGG 0.533723 3_4:-45502145 Msa0502800:CDS
AAGACAAGGTAACGTGCTTG+TGG 0.535072 3_4:-45502116 Msa0502800:CDS
CTTGTGGCCGGTAATACTCT+TGG 0.540316 3_4:-45502061 Msa0502800:CDS
CCTTCTGTGATCCGGAACTC+AGG 0.545760 3_4:-45501782 Msa0502800:CDS
CTACCAGGAAAGTGGTAGAA+TGG 0.552394 3_4:-45501675 Msa0502800:CDS
GGCGAATGCTGTTGAAATTG+TGG 0.555096 3_4:-45501897 Msa0502800:CDS
AAAATTGGTAGAAACAGTCT+TGG 0.557659 3_4:-45500177 Msa0502800:three_prime_UTR
CTCGTTCTACATAAAGATCT+AGG 0.558292 3_4:+45500341 None:intergenic
TTGGTGAGACTTCTTAGAGA+AGG 0.565763 3_4:-45501869 Msa0502800:CDS
AGAACATTTGTTGCAACTAA+CGG 0.565795 3_4:-45502352 Msa0502800:CDS
GTGTTGGTAACATGATTTCG+AGG 0.566458 3_4:-45502177 Msa0502800:CDS
ACTTATAATTATTACTGATG+AGG 0.567458 3_4:-45500050 Msa0502800:three_prime_UTR
TACAACATACATCTATGATA+GGG 0.569446 3_4:+45500203 None:intergenic
GCGAATGCTGTTGAAATTGT+GGG 0.576159 3_4:-45501896 Msa0502800:CDS
GAAAGTGGTAGAATGGTCCT+TGG 0.580251 3_4:-45501668 Msa0502800:CDS
TTGTATATTGGCTGTTGCAG+AGG 0.581097 3_4:-45501918 Msa0502800:CDS
AGGTTGTTGCCTCGTGCCGA+GGG 0.584981 3_4:-45502436 Msa0502800:CDS
CAGATGAGCTACCAGGAAAG+TGG 0.586307 3_4:-45501683 Msa0502800:CDS
AACACTACAAAACTTGTGGC+CGG 0.587605 3_4:-45502073 Msa0502800:CDS
GCTATTGAAATGGGCGAACA+TGG 0.595334 3_4:-45502478 None:intergenic
TTGGTTGGTCTTTCAAGACA+AGG 0.607223 3_4:-45502130 Msa0502800:CDS
GGCGTGTCAATCGATTGGAT+TGG 0.609217 3_4:-45502149 Msa0502800:CDS
GATAATATATCATGATACAA+TGG 0.610806 3_4:+45501545 None:intergenic
CAATATACAAAACACATCCT+CGG 0.611174 3_4:+45501931 None:intergenic
CCTGAGTTCCGGATCACAGA+AGG 0.612592 3_4:+45501782 None:intergenic
TGTTGGTAACATGATTTCGA+GGG 0.613350 3_4:-45502176 Msa0502800:CDS
GCTTCAGATGGAAGTTAGGA+GGG 0.632267 3_4:-45502245 Msa0502800:CDS
TGGGGAACTAAGAAATAATG+TGG 0.637165 3_4:-45501597 Msa0502800:CDS
TCGATGAATCAGAAACAACC+AGG 0.638423 3_4:-45500003 Msa0502800:three_prime_UTR
CATGGCTCAACAACTCAACA+AGG 0.647415 3_4:+45501747 None:intergenic
GAATTGCTCTTGCATCACCA+AGG 0.647653 3_4:+45501651 None:intergenic
AGATAACACTACAAAACTTG+TGG 0.650577 3_4:-45502077 Msa0502800:CDS
TAGGGAGTGAAACTGATACA+AGG 0.660556 3_4:+45500221 None:intergenic
ACATTTGTTGCAACTAACGG+TGG 0.663331 3_4:-45502349 Msa0502800:CDS
TGGGGCTATACCTCTTTATG+CGG 0.668733 3_4:-45501993 Msa0502800:CDS
GAAACAACCAGGTTTAATCG+AGG 0.672561 3_4:-45499992 None:intergenic
CAGACCAATTGCTCAAGCTG+GGG 0.674341 3_4:-45502011 Msa0502800:CDS
TTGGTCTGATGTAATCAACA+TGG 0.683529 3_4:+45502026 None:intergenic
TTTACTACATGACGAGACTG+GGG 0.685708 3_4:-45501615 Msa0502800:CDS
ATTCATCGAGAATAACACAA+AGG 0.688249 3_4:+45500017 None:intergenic
TTCTGGTAAGGAGATAGCCG+AGG 0.692171 3_4:-45501948 Msa0502800:CDS
TGTGATCCGGAACTCAGGTG+CGG 0.706175 3_4:-45501777 Msa0502800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAATACAAAATGATTT+GGG + chr3_4:45501313-45501332 None:intergenic 10.0%
!! TTAAATAATACAAAATGATT+TGG + chr3_4:45501314-45501333 None:intergenic 10.0%
!!! TTTTTATTGATTTTGATAAA+GGG + chr3_4:45501758-45501777 None:intergenic 10.0%
!! AAATAAAGAAAAAGATTTCT+GGG - chr3_4:45500747-45500766 Msa0502800:intron 15.0%
!!! AAAATATTGTTACTTTCTTA+AGG + chr3_4:45501459-45501478 None:intergenic 15.0%
!!! TTTTATTGATTTTGATAAAG+GGG + chr3_4:45501757-45501776 None:intergenic 15.0%
!!! CTTTTTATTGATTTTGATAA+AGG + chr3_4:45501759-45501778 None:intergenic 15.0%
!! TGTTGTATAATTTATAAAAG+AGG - chr3_4:45502314-45502333 Msa0502800:CDS 15.0%
!! GTTGTATAATTTATAAAAGA+GGG - chr3_4:45502315-45502334 Msa0502800:CDS 15.0%
!! TAATTATAAGTTACAACAAT+AGG + chr3_4:45502403-45502422 None:intergenic 15.0%
!! GAAATAAAGAAAAAGATTTC+TGG - chr3_4:45500746-45500765 Msa0502800:intron 20.0%
!! AATAAAGAAAAAGATTTCTG+GGG - chr3_4:45500748-45500767 Msa0502800:intron 20.0%
!! GATAATATATCATGATACAA+TGG + chr3_4:45500920-45500939 None:intergenic 20.0%
!! AGATTGATTAAACATGATTT+GGG + chr3_4:45501063-45501082 None:intergenic 20.0%
!! AAGATTGATTAAACATGATT+TGG + chr3_4:45501064-45501083 None:intergenic 20.0%
!!! ATTTTCTTCTTATAAGTGTA+GGG - chr3_4:45501672-45501691 Msa0502800:CDS 20.0%
!!! TTTATTGATTTTGATAAAGG+GGG + chr3_4:45501756-45501775 None:intergenic 20.0%
!! ATCTCTTCATATTCTTTATA+CGG + chr3_4:45501787-45501806 None:intergenic 20.0%
!! ATCATGTTTAATTATCTTAC+AGG - chr3_4:45502170-45502189 Msa0502800:CDS 20.0%
!! ATGTATGTTGTAATGAAAAT+TGG - chr3_4:45502270-45502289 Msa0502800:CDS 20.0%
!! ACTTATAATTATTACTGATG+AGG - chr3_4:45502412-45502431 Msa0502800:CDS 20.0%
! TGTAGTAAATCAATAAGAAC+CGG + chr3_4:45500836-45500855 None:intergenic 25.0%
! TCATGTTTAATCAATCTTAC+AGG - chr3_4:45501066-45501085 Msa0502800:intron 25.0%
! TTGATCTTTACATAGATTTC+CGG - chr3_4:45501095-45501114 Msa0502800:intron 25.0%
!! ATATATACTCGTTTGTTAAC+CGG + chr3_4:45501117-45501136 None:intergenic 25.0%
!! AGCATTTTCTTACAAGTAAA+TGG + chr3_4:45501140-45501159 None:intergenic 25.0%
! GAGGTGACAAATAATAAAAT+TGG + chr3_4:45501250-45501269 None:intergenic 25.0%
! CTACACTTATAAGAAGAAAA+TGG + chr3_4:45501673-45501692 None:intergenic 25.0%
!! CATTTTCTTCTTATAAGTGT+AGG - chr3_4:45501671-45501690 Msa0502800:CDS 25.0%
!! ACTAAGAAATATTGTTGTTG+AGG - chr3_4:45501978-45501997 Msa0502800:CDS 25.0%
! TAAAGGATCTTCATGAAAAT+TGG + chr3_4:45502010-45502029 None:intergenic 25.0%
! AAGATAATTAAACATGATCC+AGG + chr3_4:45502168-45502187 None:intergenic 25.0%
! TACAACATACATCTATGATA+GGG + chr3_4:45502262-45502281 None:intergenic 25.0%
! TTACAACATACATCTATGAT+AGG + chr3_4:45502263-45502282 None:intergenic 25.0%
!! AGTGTTGATAGAATGTTTGT+TGG - chr3_4:45500061-45500080 Msa0502800:three_prime_UTR 30.0%
! AGAACATTTGTTGCAACTAA+CGG - chr3_4:45500110-45500129 Msa0502800:three_prime_UTR 30.0%
!!! AGCACTTTGTTTTATTGTTG+AGG - chr3_4:45500256-45500275 Msa0502800:three_prime_UTR 30.0%
AGATAACACTACAAAACTTG+TGG - chr3_4:45500385-45500404 Msa0502800:intron 30.0%
CAATATACAAAACACATCCT+CGG + chr3_4:45500534-45500553 None:intergenic 30.0%
ACTAAGAAATAATGTGGTTG+AGG - chr3_4:45500871-45500890 Msa0502800:intron 30.0%
TTCTGCAAAATTCTGAATGA+AGG + chr3_4:45500959-45500978 None:intergenic 30.0%
ATTACTTTCCTCATCAGTAA+TGG + chr3_4:45501194-45501213 None:intergenic 30.0%
! TAATACAAAATGATTTGGGC+AGG + chr3_4:45501309-45501328 None:intergenic 30.0%
AATGGGATTGTAGAACTTTA+AGG + chr3_4:45501386-45501405 None:intergenic 30.0%
TTAAAGTTCTACAATCCCAT+TGG - chr3_4:45501385-45501404 Msa0502800:intron 30.0%
TAAAGTTCTACAATCCCATT+GGG - chr3_4:45501386-45501405 Msa0502800:intron 30.0%
TGTTAAACAGAGTATGACAA+CGG + chr3_4:45501424-45501443 None:intergenic 30.0%
! GACTATGGTTTAGAATTAGA+CGG + chr3_4:45501644-45501663 None:intergenic 30.0%
ATATTTCTTAGTTCACTCAG+TGG + chr3_4:45501970-45501989 None:intergenic 30.0%
CAGATAATCAATCAGTGTAA+AGG + chr3_4:45502027-45502046 None:intergenic 30.0%
TTCTGCATATATCAGAATGA+AGG + chr3_4:45502064-45502083 None:intergenic 30.0%
AAAATTGGTAGAAACAGTCT+TGG - chr3_4:45502285-45502304 Msa0502800:CDS 30.0%
GTATATAGTCATTTGCTTGT+AGG - chr3_4:45502339-45502358 Msa0502800:CDS 30.0%
ATTCATCGAGAATAACACAA+AGG + chr3_4:45502448-45502467 None:intergenic 30.0%
!! CACTTTTTGCATTTTTCCCT+CGG + chr3_4:45500045-45500064 None:intergenic 35.0%
!! TGTTGGTAACATGATTTCGA+GGG - chr3_4:45500286-45500305 Msa0502800:exon 35.0%
TAACATGATTTCGAGGGAAA+GGG - chr3_4:45500292-45500311 Msa0502800:exon 35.0%
TTGGTCTGATGTAATCAACA+TGG + chr3_4:45500439-45500458 None:intergenic 35.0%
! CGAGGATGTGTTTTGTATAT+TGG - chr3_4:45500532-45500551 Msa0502800:intron 35.0%
TGTTGAAATTGTGGGTCATT+TGG - chr3_4:45500574-45500593 Msa0502800:intron 35.0%
! TCTTTATTTCTTCAGTCCCA+TGG + chr3_4:45500736-45500755 None:intergenic 35.0%
ATTTACTACATGACGAGACT+GGG - chr3_4:45500846-45500865 Msa0502800:intron 35.0%
TGGGGAACTAAGAAATAATG+TGG - chr3_4:45500865-45500884 Msa0502800:intron 35.0%
! GGCTCTTTCTAATTTTCATG+AGG - chr3_4:45500892-45500911 Msa0502800:intron 35.0%
TATGACAACGGTTGAACTAA+AGG + chr3_4:45501412-45501431 None:intergenic 35.0%
TCTGTTTAACAGAGAGCTTA+TGG - chr3_4:45501433-45501452 Msa0502800:intron 35.0%
AGAAAATGGAGTAGTGACTA+TGG + chr3_4:45501659-45501678 None:intergenic 35.0%
AAATGTGTGTCGCAAAATGT+TGG + chr3_4:45501721-45501740 None:intergenic 35.0%
! TTTTGCGACACACATTTTGA+CGG - chr3_4:45501724-45501743 Msa0502800:CDS 35.0%
ATTCGTATTCATGCATCGAA+TGG - chr3_4:45502091-45502110 Msa0502800:CDS 35.0%
TTCGTATTCATGCATCGAAT+GGG - chr3_4:45502092-45502111 Msa0502800:CDS 35.0%
CTCGTTCTACATAAAGATCT+AGG + chr3_4:45502124-45502143 None:intergenic 35.0%
! CTTTTACAAAGCAGGTTGTT+GGG - chr3_4:45502211-45502230 Msa0502800:CDS 35.0%
GTGTTGCACGTTCTTTGTTA+AGG - chr3_4:45500006-45500025 Msa0502800:three_prime_UTR 40.0%
! ACATTTGTTGCAACTAACGG+TGG - chr3_4:45500113-45500132 Msa0502800:three_prime_UTR 40.0%
!! GTTAGAGATTTTGAGTGCGT+TGG - chr3_4:45500193-45500212 Msa0502800:three_prime_UTR 40.0%
!! TGTTTTATTGTTGAGGCTGC+TGG - chr3_4:45500263-45500282 Msa0502800:three_prime_UTR 40.0%
!! GTGTTGGTAACATGATTTCG+AGG - chr3_4:45500285-45500304 Msa0502800:exon 40.0%
GTAACATGATTTCGAGGGAA+AGG - chr3_4:45500291-45500310 Msa0502800:exon 40.0%
!! TGTCAATCGATTGGATTGGT+TGG - chr3_4:45500317-45500336 Msa0502800:CDS 40.0%
!! TTGGTTGGTCTTTCAAGACA+AGG - chr3_4:45500332-45500351 Msa0502800:CDS 40.0%
CTTGTGGAGTTGTTTAGTGA+TGG - chr3_4:45500362-45500381 Msa0502800:CDS 40.0%
AACACTACAAAACTTGTGGC+CGG - chr3_4:45500389-45500408 Msa0502800:intron 40.0%
!! TAGCACACCAAGAGTATTAC+CGG + chr3_4:45500411-45500430 None:intergenic 40.0%
CTTCAAGGAGATGATGTTTC+TGG - chr3_4:45500497-45500516 Msa0502800:intron 40.0%
!! AGGAGATGATGTTTCTGGTA+AGG - chr3_4:45500502-45500521 Msa0502800:intron 40.0%
! TTGTATATTGGCTGTTGCAG+AGG - chr3_4:45500544-45500563 Msa0502800:intron 40.0%
GCGAATGCTGTTGAAATTGT+GGG - chr3_4:45500566-45500585 Msa0502800:intron 40.0%
TTGGTGAGACTTCTTAGAGA+AGG - chr3_4:45500593-45500612 Msa0502800:intron 40.0%
! TTGTTGAGTTGTTGAGCCAT+GGG - chr3_4:45500717-45500736 Msa0502800:intron 40.0%
! TTTTTCTCAGATGAGCTACC+AGG - chr3_4:45500772-45500791 Msa0502800:intron 40.0%
GATTTACTACATGACGAGAC+TGG - chr3_4:45500845-45500864 Msa0502800:intron 40.0%
TTTACTACATGACGAGACTG+GGG - chr3_4:45500847-45500866 Msa0502800:intron 40.0%
CGATTATCGAGTAGCTCATT+TGG - chr3_4:45501035-45501054 Msa0502800:intron 40.0%
AAATGCCACCATTACTGATG+AGG - chr3_4:45501183-45501202 Msa0502800:intron 40.0%
ACTTTCCTCATCAGTAATGG+TGG + chr3_4:45501191-45501210 None:intergenic 40.0%
GACTCATCGGGAATAATACA+AGG + chr3_4:45501219-45501238 None:intergenic 40.0%
TATTCCCGATGAGTCAGAAT+CGG - chr3_4:45501224-45501243 Msa0502800:intron 40.0%
TTTGTCACCTCATTTCAGTG+AGG - chr3_4:45501259-45501278 Msa0502800:intron 40.0%
GTCCAAGGAGAATTGCATTT+CGG - chr3_4:45501281-45501300 Msa0502800:intron 40.0%
AAATTGAGCTCACTCAGTGT+TGG + chr3_4:45501927-45501946 None:intergenic 40.0%
! TCTTGACGCTTTTACAAAGC+AGG - chr3_4:45502203-45502222 Msa0502800:CDS 40.0%
! GCTTTTACAAAGCAGGTTGT+TGG - chr3_4:45502210-45502229 Msa0502800:CDS 40.0%
TAGGGAGTGAAACTGATACA+AGG + chr3_4:45502244-45502263 None:intergenic 40.0%
TCGATGAATCAGAAACAACC+AGG - chr3_4:45502459-45502478 Msa0502800:CDS 40.0%
GCTTCAGATGGAAGTTAGGA+GGG - chr3_4:45500217-45500236 Msa0502800:three_prime_UTR 45.0%
AGGAGGGAGCTCATTAGATT+AGG - chr3_4:45500233-45500252 Msa0502800:three_prime_UTR 45.0%
AAGACAAGGTAACGTGCTTG+TGG - chr3_4:45500346-45500365 Msa0502800:CDS 45.0%
ATCAGACCAATTGCTCAAGC+TGG - chr3_4:45500449-45500468 Msa0502800:intron 45.0%
TCAGACCAATTGCTCAAGCT+GGG - chr3_4:45500450-45500469 Msa0502800:intron 45.0%
TGGGGCTATACCTCTTTATG+CGG - chr3_4:45500469-45500488 Msa0502800:intron 45.0%
TGAAGAAGCTCCGCATAAAG+AGG + chr3_4:45500482-45500501 None:intergenic 45.0%
CTTTATGCGGAGCTTCTTCA+AGG - chr3_4:45500482-45500501 Msa0502800:intron 45.0%
GGCGAATGCTGTTGAAATTG+TGG - chr3_4:45500565-45500584 Msa0502800:intron 45.0%
AGCAGCTGTCGATGTGATAT+GGG - chr3_4:45500634-45500653 Msa0502800:intron 45.0%
CATGGCTCAACAACTCAACA+AGG + chr3_4:45500718-45500737 None:intergenic 45.0%
! CTTGTTGAGTTGTTGAGCCA+TGG - chr3_4:45500716-45500735 Msa0502800:intron 45.0%
CTACCAGGAAAGTGGTAGAA+TGG - chr3_4:45500787-45500806 Msa0502800:intron 45.0%
GAAAGTGGTAGAATGGTCCT+TGG - chr3_4:45500794-45500813 Msa0502800:intron 45.0%
GAATTGCTCTTGCATCACCA+AGG + chr3_4:45500814-45500833 None:intergenic 45.0%
! TGGTGATGCAAGAGCAATTC+CGG - chr3_4:45500814-45500833 Msa0502800:intron 45.0%
! ATTGGCCGATTCTGACTCAT+CGG + chr3_4:45501232-45501251 None:intergenic 45.0%
AGCCGAAATGCAATTCTCCT+TGG + chr3_4:45501286-45501305 None:intergenic 45.0%
GGTTGAACTAAAGGCCCAAT+GGG + chr3_4:45501403-45501422 None:intergenic 45.0%
TCTTAGTTCACTCAGTGGAG+AGG + chr3_4:45501965-45501984 None:intergenic 45.0%
!! CGAGTGTTGAGCAACTCATT+TGG - chr3_4:45502140-45502159 Msa0502800:CDS 45.0%
TGAGCAACTCATTTGGATCC+TGG - chr3_4:45502147-45502166 Msa0502800:CDS 45.0%
TGGCGCTTCAGATGGAAGTT+AGG - chr3_4:45500213-45500232 Msa0502800:three_prime_UTR 50.0%
CGCTTCAGATGGAAGTTAGG+AGG - chr3_4:45500216-45500235 Msa0502800:three_prime_UTR 50.0%
ATTGTTGAGGCTGCTGGTGT+TGG - chr3_4:45500269-45500288 Msa0502800:three_prime_UTR 50.0%
! GAAAGGGCGTGTCAATCGAT+TGG - chr3_4:45500308-45500327 Msa0502800:CDS 50.0%
GGCGTGTCAATCGATTGGAT+TGG - chr3_4:45500313-45500332 Msa0502800:CDS 50.0%
CTTGTGGCCGGTAATACTCT+TGG - chr3_4:45500401-45500420 Msa0502800:intron 50.0%
CAGACCAATTGCTCAAGCTG+GGG - chr3_4:45500451-45500470 Msa0502800:intron 50.0%
ATAGCCCCAGCTTGAGCAAT+TGG + chr3_4:45500458-45500477 None:intergenic 50.0%
TTCTGGTAAGGAGATAGCCG+AGG - chr3_4:45500514-45500533 Msa0502800:intron 50.0%
CAGCAGCTGTCGATGTGATA+TGG - chr3_4:45500633-45500652 Msa0502800:intron 50.0%
AGCACACACCTTCTGTGATC+CGG - chr3_4:45500672-45500691 Msa0502800:intron 50.0%
TAAAAGCCGCACCTGAGTTC+CGG + chr3_4:45500694-45500713 None:intergenic 50.0%
CAGATGAGCTACCAGGAAAG+TGG - chr3_4:45500779-45500798 Msa0502800:intron 50.0%
GGACCATTCTACCACTTTCC+TGG + chr3_4:45500793-45500812 None:intergenic 50.0%
! TTGGCCGATTCTGACTCATC+GGG + chr3_4:45501231-45501250 None:intergenic 50.0%
CCTTGGACCTCACTGAAATG+AGG + chr3_4:45501269-45501288 None:intergenic 50.0%
CCTCATTTCAGTGAGGTCCA+AGG - chr3_4:45501266-45501285 Msa0502800:intron 50.0%
CGGTTGAACTAAAGGCCCAA+TGG + chr3_4:45501404-45501423 None:intergenic 50.0%
TAGACGGTGCTTAGTGCGAA+AGG + chr3_4:45501628-45501647 None:intergenic 50.0%
AGACCAAAGAAGTGACTCGC+AGG + chr3_4:45501698-45501717 None:intergenic 50.0%
GACAACAACAACAGGCGGTT+GGG + chr3_4:45501830-45501849 None:intergenic 50.0%
GCTTGGACAACAACAACAGG+CGG + chr3_4:45501835-45501854 None:intergenic 50.0%
!! CTGGCTTGGACAACAACAAC+AGG + chr3_4:45501838-45501857 None:intergenic 50.0%
GCTGTTGTTAGAGTCCATGG+CGG + chr3_4:45501875-45501894 None:intergenic 50.0%
GAGGCTGTTGTTAGAGTCCA+TGG + chr3_4:45501878-45501897 None:intergenic 50.0%
!! AGTGTTGGCGTGGTTAGAGT+GGG + chr3_4:45501912-45501931 None:intergenic 50.0%
! TGCATTTTTCCCTCGGCACG+AGG + chr3_4:45500038-45500057 None:intergenic 55.0%
CCTGAGTTCCGGATCACAGA+AGG + chr3_4:45500683-45500702 None:intergenic 55.0%
CCTTCTGTGATCCGGAACTC+AGG - chr3_4:45500680-45500699 Msa0502800:intron 55.0%
TGTGATCCGGAACTCAGGTG+CGG - chr3_4:45500685-45500704 Msa0502800:intron 55.0%
ACAACAACAGGCGGTTGGGA+GGG + chr3_4:45501826-45501845 None:intergenic 55.0%
AACAACAACAGGCGGTTGGG+AGG + chr3_4:45501827-45501846 None:intergenic 55.0%
GGACAACAACAACAGGCGGT+TGG + chr3_4:45501831-45501850 None:intergenic 55.0%
GTTGTTAGAGTCCATGGCGG+AGG + chr3_4:45501872-45501891 None:intergenic 55.0%
! GTGTTGGCGTGGTTAGAGTG+GGG + chr3_4:45501911-45501930 None:intergenic 55.0%
!! CAGTGTTGGCGTGGTTAGAG+TGG + chr3_4:45501913-45501932 None:intergenic 55.0%
AAGGTTGTTGCCTCGTGCCG+AGG - chr3_4:45500025-45500044 Msa0502800:three_prime_UTR 60.0%
AGGTTGTTGCCTCGTGCCGA+GGG - chr3_4:45500026-45500045 Msa0502800:three_prime_UTR 60.0%
! GAGTGCGTTGGCGCTTCAGA+TGG - chr3_4:45500205-45500224 Msa0502800:three_prime_UTR 60.0%
TGCTCGCTCGTTGCACGAAG+AGG + chr3_4:45500992-45501011 None:intergenic 60.0%
GGGCCTGCGAGTCACTTCTT+TGG - chr3_4:45501692-45501711 Msa0502800:CDS 60.0%
! TAGAGTCCATGGCGGAGGTG+TGG + chr3_4:45501867-45501886 None:intergenic 60.0%
AGAGTGGGGACTGGAGACAG+AGG + chr3_4:45501897-45501916 None:intergenic 60.0%
!! GAGCTCACTCAGTGTTGGCG+TGG + chr3_4:45501922-45501941 None:intergenic 60.0%
AACAGGCGGTTGGGAGGGAG+AGG + chr3_4:45501821-45501840 None:intergenic 65.0%
! AGGTGTGGTGGCGACTGGCT+TGG + chr3_4:45501852-45501871 None:intergenic 65.0%
! AGTCCATGGCGGAGGTGTGG+TGG + chr3_4:45501864-45501883 None:intergenic 65.0%
GGCGTGGTTAGAGTGGGGAC+TGG + chr3_4:45501906-45501925 None:intergenic 65.0%
TCGCCACCACACCTCCGCCA+TGG - chr3_4:45501858-45501877 Msa0502800:CDS 70.0%
! GGCGGAGGTGTGGTGGCGAC+TGG + chr3_4:45501857-45501876 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 45499993 45502491 45499993 ID=Msa0502800;Name=Msa0502800
chr3_4 mRNA 45499993 45502491 45499993 ID=Msa0502800-mRNA-1;Parent=Msa0502800;Name=Msa0502800-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0.5|1|0|0|2|311|350
chr3_4 exon 45499993 45500402 45499993 ID=Msa0502800-mRNA-1:exon:9675;Parent=Msa0502800-mRNA-1
chr3_4 exon 45501538 45502491 45501538 ID=Msa0502800-mRNA-1:exon:9674;Parent=Msa0502800-mRNA-1
chr3_4 CDS 45501538 45502491 45501538 ID=Msa0502800-mRNA-1:cds;Parent=Msa0502800-mRNA-1
chr3_4 CDS 45500304 45500402 45500304 ID=Msa0502800-mRNA-1:cds;Parent=Msa0502800-mRNA-1
chr3_4 three_prime_UTR 45499993 45500303 45499993 ID=Msa0502800-mRNA-1:three_prime_utr;Parent=Msa0502800-mRNA-1
Gene Sequence

>Msa0502800

ATGGGCGAACATGGTGTTGCACGTTCTTTGTTAAGGTTGTTGCCTCGTGCCGAGGGAAAAATGCAAAAAGTGTTGATAGAATGTTTGTTGGTTGTTGTAAGTTTTTGTGATAATAGTAGAACATTTGTTGCAACTAACGGTGGTTTAGAGATTATTATTAGTTTGTTGAGTAGTTGTAGCGATGATGTTAGATTGTATTTGTTAGAGATTTTGAGTGCGTTGGCGCTTCAGATGGAAGTTAGGAGGGAGCTCATTAGATTAGGAGCACTTTGTTTTATTGTTGAGGCTGCTGGTGTTGGTAACATGATTTCGAGGGAAAGGGCGTGTCAATCGATTGGATTGGTTGGTCTTTCAAGACAAGGTAACGTGCTTGTGGAGTTGTTTAGTGATGGAGATAACACTACAAAACTTGTGGCCGGTAATACTCTTGGTGTGCTATTGAACCATGTTGATTACATCAGACCAATTGCTCAAGCTGGGGCTATACCTCTTTATGCGGAGCTTCTTCAAGGAGATGATGTTTCTGGTAAGGAGATAGCCGAGGATGTGTTTTGTATATTGGCTGTTGCAGAGGCGAATGCTGTTGAAATTGTGGGTCATTTGGTGAGACTTCTTAGAGAAGGTGATGACGAAGCTAAAGCAGCAGCTGTCGATGTGATATGGGATCTGTTGCGATACAAGCACACACCTTCTGTGATCCGGAACTCAGGTGCGGCTTTTATCCTTGTTGAGTTGTTGAGCCATGGGACTGAAGAAATAAAGAAAAAGATTTCTGGGGCTTTTTCTCAGATGAGCTACCAGGAAAGTGGTAGAATGGTCCTTGGTGATGCAAGAGCAATTCCGGTTCTTATTGATTTACTACATGACGAGACTGGGGAACTAAGAAATAATGTGGTTGAGGCTCTTTCTAATTTTCATGAGGATCCATTGTATCATGATATATTATCTGATGTAAATAGACTAATTCGTATTCATGCATCGAATGGGCATATGCCTAGATCTTTATGTAGAACGAGTGTTGAGCAACTCATTTGGATCCTGGATCATGTTTAA
Protein sequence

>Msa0502800

MGEHGVARSLLRLLPRAEGKMQKVLIECLLVVVSFCDNSRTFVATNGGLEIIISLLSSCSDDVRLYLLEILSALALQMEVRRELIRLGALCFIVEAAGVGNMISRERACQSIGLVGLSRQGNVLVELFSDGDNTTKLVAGNTLGVLLNHVDYIRPIAQAGAIPLYAELLQGDDVSGKEIAEDVFCILAVAEANAVEIVGHLVRLLREGDDEAKAAAVDVIWDLLRYKHTPSVIRNSGAAFILVELLSHGTEEIKKKISGAFSQMSYQESGRMVLGDARAIPVLIDLLHDETGELRNNVVEALSNFHEDPLYHDILSDVNRLIRIHASNGHMPRSLCRTSVEQLIWILDHV*