AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0362940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0362940 MtrunA17_Chr3g0101311 85.042 361 34 2 1 341 100 460 0.0 600
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0362940 AT3G20170.1 48.919 370 159 5 1 343 102 468 2.31e-104 315

Find 89 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAAGGAGATGATGTTTC+TGG 0.181044 3_1:-54797287 Msa0362940:CDS
ATCATGTTTAATTATCTTAC+AGG 0.218306 3_1:-54795614 Msa0362940:exon
CAGCAGCTGTCGATGTGATA+TGG 0.278624 3_1:-54797151 Msa0362940:CDS
TGGTGATGCAAGAGCAATTC+CGG 0.352200 3_1:-54796970 Msa0362940:CDS
GGACCATTCTACCACTTTCC+TGG 0.363654 3_1:+54796994 None:intergenic
TTACAACATACATCTATGAT+AGG 0.385514 3_1:+54795524 None:intergenic
CGAGTGTTGAGCAACTCATT+TGG 0.385896 3_1:-54795644 Msa0362940:CDS
TGGCGCTTCAGATGGAAGTT+AGG 0.404983 3_1:-54797571 Msa0362940:CDS
GATTTACTACATGACGAGAC+TGG 0.405844 3_1:-54796939 Msa0362940:CDS
ATAGCCCCAGCTTGAGCAAT+TGG 0.406485 3_1:+54797329 None:intergenic
GTATATAGTCATTTGCTTGT+AGG 0.407308 3_1:-54795445 Msa0362940:three_prime_UTR
ATGTATGTTGTAATGAAAAT+TGG 0.409597 3_1:-54795514 Msa0362940:three_prime_UTR
TAAAAGCCGCACCTGAGTTC+CGG 0.410207 3_1:+54797093 None:intergenic
AGGAGGGAGCTCATTAGATT+AGG 0.412597 3_1:-54797551 Msa0362940:CDS
TAATTATAAGTTACAACAAT+AGG 0.413182 3_1:+54795384 None:intergenic
GTTGTATAATTTATAAAAGA+GGG 0.413839 3_1:-54795469 Msa0362940:three_prime_UTR
TCTTTATTTCTTCAGTCCCA+TGG 0.430621 3_1:+54797051 None:intergenic
TAGCACACCAAGAGTATTAC+CGG 0.430738 3_1:+54797376 None:intergenic
ATCAGACCAATTGCTCAAGC+TGG 0.432583 3_1:-54797335 Msa0362940:CDS
CTTTATGCGGAGCTTCTTCA+AGG 0.433825 3_1:-54797302 Msa0362940:CDS
TGTTGAAATTGTGGGTCATT+TGG 0.446283 3_1:-54797210 Msa0362940:CDS
GTGTTGCACGTTCTTTGTTA+AGG 0.459610 3_1:-54797778 Msa0362940:CDS
TGTTGTATAATTTATAAAAG+AGG 0.462397 3_1:-54795470 Msa0362940:three_prime_UTR
AGTGTTGATAGAATGTTTGT+TGG 0.463303 3_1:-54797723 Msa0362940:CDS
TGAGCAACTCATTTGGATCC+TGG 0.466205 3_1:-54795637 Msa0362940:CDS
TGTAGTAAATCAATAAGAAC+CGG 0.466912 3_1:+54796951 None:intergenic
CTTTGAACCTCGATTAAACC+TGG 0.467265 3_1:+54795307 None:intergenic
AGCAGCTGTCGATGTGATAT+GGG 0.479152 3_1:-54797150 Msa0362940:CDS
CTTGTTGAGTTGTTGAGCCA+TGG 0.489304 3_1:-54797068 Msa0362940:CDS
AGGAGATGATGTTTCTGGTA+AGG 0.494027 3_1:-54797282 Msa0362940:CDS
CGCTTCAGATGGAAGTTAGG+AGG 0.496594 3_1:-54797568 Msa0362940:CDS
ATTGTTGAGGCTGCTGGTGT+TGG 0.498526 3_1:-54797515 Msa0362940:CDS
AGCACACACCTTCTGTGATC+CGG 0.498591 3_1:-54797112 Msa0362940:CDS
ATTTACTACATGACGAGACT+GGG 0.505397 3_1:-54796938 Msa0362940:CDS
AAGGTTGTTGCCTCGTGCCG+AGG 0.506613 3_1:-54797759 Msa0362940:CDS
TTCGTATTCATGCATCGAAT+GGG 0.509754 3_1:-54795692 Msa0362940:CDS
TAACATGATTTCGAGGGAAA+GGG 0.510380 3_1:-54797492 Msa0362940:CDS
ACTAAGAAATAATGTGGTTG+AGG 0.515775 3_1:-54796913 Msa0362940:CDS
TGAAGAAGCTCCGCATAAAG+AGG 0.516045 3_1:+54797305 None:intergenic
TCAGACCAATTGCTCAAGCT+GGG 0.516253 3_1:-54797334 Msa0362940:CDS
TTGTTGAGTTGTTGAGCCAT+GGG 0.519539 3_1:-54797067 Msa0362940:CDS
GAGTGCGTTGGCGCTTCAGA+TGG 0.520748 3_1:-54797579 Msa0362940:CDS
AAGATAATTAAACATGATCC+AGG 0.523474 3_1:+54795619 None:intergenic
CTTGTGGAGTTGTTTAGTGA+TGG 0.523872 3_1:-54797422 Msa0362940:CDS
GTAACATGATTTCGAGGGAA+AGG 0.527617 3_1:-54797493 Msa0362940:CDS
GAAAGGGCGTGTCAATCGAT+TGG 0.529311 3_1:-54797476 Msa0362940:CDS
ATTCGTATTCATGCATCGAA+TGG 0.531293 3_1:-54795693 Msa0362940:CDS
TGTCAATCGATTGGATTGGT+TGG 0.533723 3_1:-54797467 Msa0362940:CDS
AAGACAAGGTAACGTGCTTG+TGG 0.535072 3_1:-54797438 Msa0362940:CDS
CTTGTGGCCGGTAATACTCT+TGG 0.540316 3_1:-54797383 Msa0362940:CDS
CCTTCTGTGATCCGGAACTC+AGG 0.545760 3_1:-54797104 Msa0362940:CDS
CTACCAGGAAAGTGGTAGAA+TGG 0.552394 3_1:-54796997 Msa0362940:CDS
GGCGAATGCTGTTGAAATTG+TGG 0.555096 3_1:-54797219 Msa0362940:CDS
AAAATTGGTAGAAACAGTCT+TGG 0.557659 3_1:-54795499 Msa0362940:three_prime_UTR
CTCGTTCTACATAAAGATCT+AGG 0.558292 3_1:+54795663 None:intergenic
TTGGTGAGACTTCTTAGAGA+AGG 0.565763 3_1:-54797191 Msa0362940:CDS
AGAACATTTGTTGCAACTAA+CGG 0.565795 3_1:-54797674 Msa0362940:CDS
GTGTTGGTAACATGATTTCG+AGG 0.566458 3_1:-54797499 Msa0362940:CDS
ACTTATAATTATTACTGATG+AGG 0.567458 3_1:-54795372 Msa0362940:three_prime_UTR
TACAACATACATCTATGATA+GGG 0.569446 3_1:+54795525 None:intergenic
GCGAATGCTGTTGAAATTGT+GGG 0.576159 3_1:-54797218 Msa0362940:CDS
GAAAGTGGTAGAATGGTCCT+TGG 0.580251 3_1:-54796990 Msa0362940:CDS
TTGTATATTGGCTGTTGCAG+AGG 0.581097 3_1:-54797240 Msa0362940:CDS
AGGTTGTTGCCTCGTGCCGA+GGG 0.584981 3_1:-54797758 Msa0362940:CDS
CAGATGAGCTACCAGGAAAG+TGG 0.586307 3_1:-54797005 Msa0362940:CDS
AACACTACAAAACTTGTGGC+CGG 0.587605 3_1:-54797395 Msa0362940:CDS
GCTATTGAAATGGGCGAACA+TGG 0.595334 3_1:-54797800 None:intergenic
TTGGTTGGTCTTTCAAGACA+AGG 0.607223 3_1:-54797452 Msa0362940:CDS
GGCGTGTCAATCGATTGGAT+TGG 0.609217 3_1:-54797471 Msa0362940:CDS
GATAATATATCATGATACAA+TGG 0.610806 3_1:+54796867 None:intergenic
CAATATACAAAACACATCCT+CGG 0.611174 3_1:+54797253 None:intergenic
CCTGAGTTCCGGATCACAGA+AGG 0.612592 3_1:+54797104 None:intergenic
TGTTGGTAACATGATTTCGA+GGG 0.613350 3_1:-54797498 Msa0362940:CDS
GCTTCAGATGGAAGTTAGGA+GGG 0.632267 3_1:-54797567 Msa0362940:CDS
TGGGGAACTAAGAAATAATG+TGG 0.637165 3_1:-54796919 Msa0362940:CDS
TCGATGAATCAGAAACAACC+AGG 0.638423 3_1:-54795325 Msa0362940:three_prime_UTR
CATGGCTCAACAACTCAACA+AGG 0.647415 3_1:+54797069 None:intergenic
GAATTGCTCTTGCATCACCA+AGG 0.647653 3_1:+54796973 None:intergenic
AGATAACACTACAAAACTTG+TGG 0.650577 3_1:-54797399 Msa0362940:CDS
TAGGGAGTGAAACTGATACA+AGG 0.660556 3_1:+54795543 None:intergenic
ACATTTGTTGCAACTAACGG+TGG 0.663331 3_1:-54797671 Msa0362940:CDS
TGGGGCTATACCTCTTTATG+CGG 0.668733 3_1:-54797315 Msa0362940:CDS
GAAACAACCAGGTTTAATCG+AGG 0.672561 3_1:-54795314 None:intergenic
CAGACCAATTGCTCAAGCTG+GGG 0.674341 3_1:-54797333 Msa0362940:CDS
TTGGTCTGATGTAATCAACA+TGG 0.683529 3_1:+54797348 None:intergenic
TTTACTACATGACGAGACTG+GGG 0.685708 3_1:-54796937 Msa0362940:CDS
ATTCATCGAGAATAACACAA+AGG 0.688249 3_1:+54795339 None:intergenic
TTCTGGTAAGGAGATAGCCG+AGG 0.692171 3_1:-54797270 Msa0362940:CDS
TGTGATCCGGAACTCAGGTG+CGG 0.706175 3_1:-54797099 Msa0362940:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAATACAAAATGATTT+GGG + chr3_1:54796635-54796654 None:intergenic 10.0%
!! TTAAATAATACAAAATGATT+TGG + chr3_1:54796636-54796655 None:intergenic 10.0%
!!! TTTTTATTGATTTTGATAAA+GGG + chr3_1:54797080-54797099 None:intergenic 10.0%
!! AAATAAAGAAAAAGATTTCT+GGG - chr3_1:54796069-54796088 Msa0362940:intron 15.0%
!!! AAAATATTGTTACTTTCTTA+AGG + chr3_1:54796781-54796800 None:intergenic 15.0%
!!! TTTTATTGATTTTGATAAAG+GGG + chr3_1:54797079-54797098 None:intergenic 15.0%
!!! CTTTTTATTGATTTTGATAA+AGG + chr3_1:54797081-54797100 None:intergenic 15.0%
!! TGTTGTATAATTTATAAAAG+AGG - chr3_1:54797636-54797655 Msa0362940:CDS 15.0%
!! GTTGTATAATTTATAAAAGA+GGG - chr3_1:54797637-54797656 Msa0362940:CDS 15.0%
!! TAATTATAAGTTACAACAAT+AGG + chr3_1:54797725-54797744 None:intergenic 15.0%
!! GAAATAAAGAAAAAGATTTC+TGG - chr3_1:54796068-54796087 Msa0362940:intron 20.0%
!! AATAAAGAAAAAGATTTCTG+GGG - chr3_1:54796070-54796089 Msa0362940:intron 20.0%
!! GATAATATATCATGATACAA+TGG + chr3_1:54796242-54796261 None:intergenic 20.0%
!! AGATTGATTAAACATGATTT+GGG + chr3_1:54796385-54796404 None:intergenic 20.0%
!! AAGATTGATTAAACATGATT+TGG + chr3_1:54796386-54796405 None:intergenic 20.0%
!!! ATTTTCTTCTTATAAGTGTA+GGG - chr3_1:54796994-54797013 Msa0362940:CDS 20.0%
!!! TTTATTGATTTTGATAAAGG+GGG + chr3_1:54797078-54797097 None:intergenic 20.0%
!! ATCTCTTCATATTCTTTATA+CGG + chr3_1:54797109-54797128 None:intergenic 20.0%
!! ATCATGTTTAATTATCTTAC+AGG - chr3_1:54797492-54797511 Msa0362940:CDS 20.0%
!! ATGTATGTTGTAATGAAAAT+TGG - chr3_1:54797592-54797611 Msa0362940:CDS 20.0%
!! ACTTATAATTATTACTGATG+AGG - chr3_1:54797734-54797753 Msa0362940:CDS 20.0%
! TGTAGTAAATCAATAAGAAC+CGG + chr3_1:54796158-54796177 None:intergenic 25.0%
! TCATGTTTAATCAATCTTAC+AGG - chr3_1:54796388-54796407 Msa0362940:intron 25.0%
! TTGATCTTTACATAGATTTC+CGG - chr3_1:54796417-54796436 Msa0362940:intron 25.0%
!! ATATATACTCGTTTGTTAAC+CGG + chr3_1:54796439-54796458 None:intergenic 25.0%
!! AGCATTTTCTTACAAGTAAA+TGG + chr3_1:54796462-54796481 None:intergenic 25.0%
! GAGGTGACAAATAATAAAAT+TGG + chr3_1:54796572-54796591 None:intergenic 25.0%
! CTACACTTATAAGAAGAAAA+TGG + chr3_1:54796995-54797014 None:intergenic 25.0%
!! CATTTTCTTCTTATAAGTGT+AGG - chr3_1:54796993-54797012 Msa0362940:CDS 25.0%
!! ACTAAGAAATATTGTTGTTG+AGG - chr3_1:54797300-54797319 Msa0362940:CDS 25.0%
! TAAAGGATCTTCATGAAAAT+TGG + chr3_1:54797332-54797351 None:intergenic 25.0%
! AAGATAATTAAACATGATCC+AGG + chr3_1:54797490-54797509 None:intergenic 25.0%
! TACAACATACATCTATGATA+GGG + chr3_1:54797584-54797603 None:intergenic 25.0%
! TTACAACATACATCTATGAT+AGG + chr3_1:54797585-54797604 None:intergenic 25.0%
!! AGTGTTGATAGAATGTTTGT+TGG - chr3_1:54795383-54795402 Msa0362940:three_prime_UTR 30.0%
! AGAACATTTGTTGCAACTAA+CGG - chr3_1:54795432-54795451 Msa0362940:three_prime_UTR 30.0%
!!! AGCACTTTGTTTTATTGTTG+AGG - chr3_1:54795578-54795597 Msa0362940:three_prime_UTR 30.0%
AGATAACACTACAAAACTTG+TGG - chr3_1:54795707-54795726 Msa0362940:intron 30.0%
CAATATACAAAACACATCCT+CGG + chr3_1:54795856-54795875 None:intergenic 30.0%
ACTAAGAAATAATGTGGTTG+AGG - chr3_1:54796193-54796212 Msa0362940:intron 30.0%
TTCTGCAAAATTCTGAATGA+AGG + chr3_1:54796281-54796300 None:intergenic 30.0%
ATTACTTTCCTCATCAGTAA+TGG + chr3_1:54796516-54796535 None:intergenic 30.0%
! TAATACAAAATGATTTGGGC+AGG + chr3_1:54796631-54796650 None:intergenic 30.0%
AATGGGATTGTAGAACTTTA+AGG + chr3_1:54796708-54796727 None:intergenic 30.0%
TTAAAGTTCTACAATCCCAT+TGG - chr3_1:54796707-54796726 Msa0362940:intron 30.0%
TAAAGTTCTACAATCCCATT+GGG - chr3_1:54796708-54796727 Msa0362940:intron 30.0%
TGTTAAACAGAGTATGACAA+CGG + chr3_1:54796746-54796765 None:intergenic 30.0%
! GACTATGGTTTAGAATTAGA+CGG + chr3_1:54796966-54796985 None:intergenic 30.0%
ATATTTCTTAGTTCACTCAG+TGG + chr3_1:54797292-54797311 None:intergenic 30.0%
CAGATAATCAATCAGTGTAA+AGG + chr3_1:54797349-54797368 None:intergenic 30.0%
TTCTGCATATATCAGAATGA+AGG + chr3_1:54797386-54797405 None:intergenic 30.0%
AAAATTGGTAGAAACAGTCT+TGG - chr3_1:54797607-54797626 Msa0362940:CDS 30.0%
GTATATAGTCATTTGCTTGT+AGG - chr3_1:54797661-54797680 Msa0362940:CDS 30.0%
ATTCATCGAGAATAACACAA+AGG + chr3_1:54797770-54797789 None:intergenic 30.0%
!! CACTTTTTGCATTTTTCCCT+CGG + chr3_1:54795367-54795386 None:intergenic 35.0%
!! TGTTGGTAACATGATTTCGA+GGG - chr3_1:54795608-54795627 Msa0362940:exon 35.0%
TAACATGATTTCGAGGGAAA+GGG - chr3_1:54795614-54795633 Msa0362940:exon 35.0%
TTGGTCTGATGTAATCAACA+TGG + chr3_1:54795761-54795780 None:intergenic 35.0%
! CGAGGATGTGTTTTGTATAT+TGG - chr3_1:54795854-54795873 Msa0362940:intron 35.0%
TGTTGAAATTGTGGGTCATT+TGG - chr3_1:54795896-54795915 Msa0362940:intron 35.0%
! TCTTTATTTCTTCAGTCCCA+TGG + chr3_1:54796058-54796077 None:intergenic 35.0%
ATTTACTACATGACGAGACT+GGG - chr3_1:54796168-54796187 Msa0362940:intron 35.0%
TGGGGAACTAAGAAATAATG+TGG - chr3_1:54796187-54796206 Msa0362940:intron 35.0%
! GGCTCTTTCTAATTTTCATG+AGG - chr3_1:54796214-54796233 Msa0362940:intron 35.0%
TATGACAACGGTTGAACTAA+AGG + chr3_1:54796734-54796753 None:intergenic 35.0%
TCTGTTTAACAGAGAGCTTA+TGG - chr3_1:54796755-54796774 Msa0362940:intron 35.0%
AGAAAATGGAGTAGTGACTA+TGG + chr3_1:54796981-54797000 None:intergenic 35.0%
AAATGTGTGTCGCAAAATGT+TGG + chr3_1:54797043-54797062 None:intergenic 35.0%
! TTTTGCGACACACATTTTGA+CGG - chr3_1:54797046-54797065 Msa0362940:CDS 35.0%
ATTCGTATTCATGCATCGAA+TGG - chr3_1:54797413-54797432 Msa0362940:CDS 35.0%
TTCGTATTCATGCATCGAAT+GGG - chr3_1:54797414-54797433 Msa0362940:CDS 35.0%
CTCGTTCTACATAAAGATCT+AGG + chr3_1:54797446-54797465 None:intergenic 35.0%
! CTTTTACAAAGCAGGTTGTT+GGG - chr3_1:54797533-54797552 Msa0362940:CDS 35.0%
GTGTTGCACGTTCTTTGTTA+AGG - chr3_1:54795328-54795347 Msa0362940:three_prime_UTR 40.0%
! ACATTTGTTGCAACTAACGG+TGG - chr3_1:54795435-54795454 Msa0362940:three_prime_UTR 40.0%
!! GTTAGAGATTTTGAGTGCGT+TGG - chr3_1:54795515-54795534 Msa0362940:three_prime_UTR 40.0%
!! TGTTTTATTGTTGAGGCTGC+TGG - chr3_1:54795585-54795604 Msa0362940:three_prime_UTR 40.0%
!! GTGTTGGTAACATGATTTCG+AGG - chr3_1:54795607-54795626 Msa0362940:exon 40.0%
GTAACATGATTTCGAGGGAA+AGG - chr3_1:54795613-54795632 Msa0362940:exon 40.0%
!! TGTCAATCGATTGGATTGGT+TGG - chr3_1:54795639-54795658 Msa0362940:CDS 40.0%
!! TTGGTTGGTCTTTCAAGACA+AGG - chr3_1:54795654-54795673 Msa0362940:CDS 40.0%
CTTGTGGAGTTGTTTAGTGA+TGG - chr3_1:54795684-54795703 Msa0362940:CDS 40.0%
AACACTACAAAACTTGTGGC+CGG - chr3_1:54795711-54795730 Msa0362940:intron 40.0%
!! TAGCACACCAAGAGTATTAC+CGG + chr3_1:54795733-54795752 None:intergenic 40.0%
CTTCAAGGAGATGATGTTTC+TGG - chr3_1:54795819-54795838 Msa0362940:intron 40.0%
!! AGGAGATGATGTTTCTGGTA+AGG - chr3_1:54795824-54795843 Msa0362940:intron 40.0%
! TTGTATATTGGCTGTTGCAG+AGG - chr3_1:54795866-54795885 Msa0362940:intron 40.0%
GCGAATGCTGTTGAAATTGT+GGG - chr3_1:54795888-54795907 Msa0362940:intron 40.0%
TTGGTGAGACTTCTTAGAGA+AGG - chr3_1:54795915-54795934 Msa0362940:intron 40.0%
! TTGTTGAGTTGTTGAGCCAT+GGG - chr3_1:54796039-54796058 Msa0362940:intron 40.0%
! TTTTTCTCAGATGAGCTACC+AGG - chr3_1:54796094-54796113 Msa0362940:intron 40.0%
GATTTACTACATGACGAGAC+TGG - chr3_1:54796167-54796186 Msa0362940:intron 40.0%
TTTACTACATGACGAGACTG+GGG - chr3_1:54796169-54796188 Msa0362940:intron 40.0%
CGATTATCGAGTAGCTCATT+TGG - chr3_1:54796357-54796376 Msa0362940:intron 40.0%
AAATGCCACCATTACTGATG+AGG - chr3_1:54796505-54796524 Msa0362940:intron 40.0%
ACTTTCCTCATCAGTAATGG+TGG + chr3_1:54796513-54796532 None:intergenic 40.0%
GACTCATCGGGAATAATACA+AGG + chr3_1:54796541-54796560 None:intergenic 40.0%
TATTCCCGATGAGTCAGAAT+CGG - chr3_1:54796546-54796565 Msa0362940:intron 40.0%
TTTGTCACCTCATTTCAGTG+AGG - chr3_1:54796581-54796600 Msa0362940:intron 40.0%
GTCCAAGGAGAATTGCATTT+CGG - chr3_1:54796603-54796622 Msa0362940:intron 40.0%
AAATTGAGCTCACTCAGTGT+TGG + chr3_1:54797249-54797268 None:intergenic 40.0%
! TCTTGACGCTTTTACAAAGC+AGG - chr3_1:54797525-54797544 Msa0362940:CDS 40.0%
! GCTTTTACAAAGCAGGTTGT+TGG - chr3_1:54797532-54797551 Msa0362940:CDS 40.0%
TAGGGAGTGAAACTGATACA+AGG + chr3_1:54797566-54797585 None:intergenic 40.0%
TCGATGAATCAGAAACAACC+AGG - chr3_1:54797781-54797800 Msa0362940:CDS 40.0%
GCTTCAGATGGAAGTTAGGA+GGG - chr3_1:54795539-54795558 Msa0362940:three_prime_UTR 45.0%
AGGAGGGAGCTCATTAGATT+AGG - chr3_1:54795555-54795574 Msa0362940:three_prime_UTR 45.0%
AAGACAAGGTAACGTGCTTG+TGG - chr3_1:54795668-54795687 Msa0362940:CDS 45.0%
ATCAGACCAATTGCTCAAGC+TGG - chr3_1:54795771-54795790 Msa0362940:intron 45.0%
TCAGACCAATTGCTCAAGCT+GGG - chr3_1:54795772-54795791 Msa0362940:intron 45.0%
TGGGGCTATACCTCTTTATG+CGG - chr3_1:54795791-54795810 Msa0362940:intron 45.0%
TGAAGAAGCTCCGCATAAAG+AGG + chr3_1:54795804-54795823 None:intergenic 45.0%
CTTTATGCGGAGCTTCTTCA+AGG - chr3_1:54795804-54795823 Msa0362940:intron 45.0%
GGCGAATGCTGTTGAAATTG+TGG - chr3_1:54795887-54795906 Msa0362940:intron 45.0%
AGCAGCTGTCGATGTGATAT+GGG - chr3_1:54795956-54795975 Msa0362940:intron 45.0%
CATGGCTCAACAACTCAACA+AGG + chr3_1:54796040-54796059 None:intergenic 45.0%
! CTTGTTGAGTTGTTGAGCCA+TGG - chr3_1:54796038-54796057 Msa0362940:intron 45.0%
CTACCAGGAAAGTGGTAGAA+TGG - chr3_1:54796109-54796128 Msa0362940:intron 45.0%
GAAAGTGGTAGAATGGTCCT+TGG - chr3_1:54796116-54796135 Msa0362940:intron 45.0%
GAATTGCTCTTGCATCACCA+AGG + chr3_1:54796136-54796155 None:intergenic 45.0%
! TGGTGATGCAAGAGCAATTC+CGG - chr3_1:54796136-54796155 Msa0362940:intron 45.0%
! ATTGGCCGATTCTGACTCAT+CGG + chr3_1:54796554-54796573 None:intergenic 45.0%
AGCCGAAATGCAATTCTCCT+TGG + chr3_1:54796608-54796627 None:intergenic 45.0%
GGTTGAACTAAAGGCCCAAT+GGG + chr3_1:54796725-54796744 None:intergenic 45.0%
TCTTAGTTCACTCAGTGGAG+AGG + chr3_1:54797287-54797306 None:intergenic 45.0%
!! CGAGTGTTGAGCAACTCATT+TGG - chr3_1:54797462-54797481 Msa0362940:CDS 45.0%
TGAGCAACTCATTTGGATCC+TGG - chr3_1:54797469-54797488 Msa0362940:CDS 45.0%
TGGCGCTTCAGATGGAAGTT+AGG - chr3_1:54795535-54795554 Msa0362940:three_prime_UTR 50.0%
CGCTTCAGATGGAAGTTAGG+AGG - chr3_1:54795538-54795557 Msa0362940:three_prime_UTR 50.0%
ATTGTTGAGGCTGCTGGTGT+TGG - chr3_1:54795591-54795610 Msa0362940:three_prime_UTR 50.0%
! GAAAGGGCGTGTCAATCGAT+TGG - chr3_1:54795630-54795649 Msa0362940:CDS 50.0%
GGCGTGTCAATCGATTGGAT+TGG - chr3_1:54795635-54795654 Msa0362940:CDS 50.0%
CTTGTGGCCGGTAATACTCT+TGG - chr3_1:54795723-54795742 Msa0362940:intron 50.0%
CAGACCAATTGCTCAAGCTG+GGG - chr3_1:54795773-54795792 Msa0362940:intron 50.0%
ATAGCCCCAGCTTGAGCAAT+TGG + chr3_1:54795780-54795799 None:intergenic 50.0%
TTCTGGTAAGGAGATAGCCG+AGG - chr3_1:54795836-54795855 Msa0362940:intron 50.0%
CAGCAGCTGTCGATGTGATA+TGG - chr3_1:54795955-54795974 Msa0362940:intron 50.0%
AGCACACACCTTCTGTGATC+CGG - chr3_1:54795994-54796013 Msa0362940:intron 50.0%
TAAAAGCCGCACCTGAGTTC+CGG + chr3_1:54796016-54796035 None:intergenic 50.0%
CAGATGAGCTACCAGGAAAG+TGG - chr3_1:54796101-54796120 Msa0362940:intron 50.0%
GGACCATTCTACCACTTTCC+TGG + chr3_1:54796115-54796134 None:intergenic 50.0%
! TTGGCCGATTCTGACTCATC+GGG + chr3_1:54796553-54796572 None:intergenic 50.0%
CCTTGGACCTCACTGAAATG+AGG + chr3_1:54796591-54796610 None:intergenic 50.0%
CCTCATTTCAGTGAGGTCCA+AGG - chr3_1:54796588-54796607 Msa0362940:intron 50.0%
CGGTTGAACTAAAGGCCCAA+TGG + chr3_1:54796726-54796745 None:intergenic 50.0%
TAGACGGTGCTTAGTGCGAA+AGG + chr3_1:54796950-54796969 None:intergenic 50.0%
AGACCAAAGAAGTGACTCGC+AGG + chr3_1:54797020-54797039 None:intergenic 50.0%
GACAACAACAACAGGCGGTT+GGG + chr3_1:54797152-54797171 None:intergenic 50.0%
GCTTGGACAACAACAACAGG+CGG + chr3_1:54797157-54797176 None:intergenic 50.0%
!! CTGGCTTGGACAACAACAAC+AGG + chr3_1:54797160-54797179 None:intergenic 50.0%
GCTGTTGTTAGAGTCCATGG+CGG + chr3_1:54797197-54797216 None:intergenic 50.0%
GAGGCTGTTGTTAGAGTCCA+TGG + chr3_1:54797200-54797219 None:intergenic 50.0%
!! AGTGTTGGCGTGGTTAGAGT+GGG + chr3_1:54797234-54797253 None:intergenic 50.0%
! TGCATTTTTCCCTCGGCACG+AGG + chr3_1:54795360-54795379 None:intergenic 55.0%
CCTGAGTTCCGGATCACAGA+AGG + chr3_1:54796005-54796024 None:intergenic 55.0%
CCTTCTGTGATCCGGAACTC+AGG - chr3_1:54796002-54796021 Msa0362940:intron 55.0%
TGTGATCCGGAACTCAGGTG+CGG - chr3_1:54796007-54796026 Msa0362940:intron 55.0%
ACAACAACAGGCGGTTGGGA+GGG + chr3_1:54797148-54797167 None:intergenic 55.0%
AACAACAACAGGCGGTTGGG+AGG + chr3_1:54797149-54797168 None:intergenic 55.0%
GGACAACAACAACAGGCGGT+TGG + chr3_1:54797153-54797172 None:intergenic 55.0%
GTTGTTAGAGTCCATGGCGG+AGG + chr3_1:54797194-54797213 None:intergenic 55.0%
! GTGTTGGCGTGGTTAGAGTG+GGG + chr3_1:54797233-54797252 None:intergenic 55.0%
!! CAGTGTTGGCGTGGTTAGAG+TGG + chr3_1:54797235-54797254 None:intergenic 55.0%
AAGGTTGTTGCCTCGTGCCG+AGG - chr3_1:54795347-54795366 Msa0362940:three_prime_UTR 60.0%
AGGTTGTTGCCTCGTGCCGA+GGG - chr3_1:54795348-54795367 Msa0362940:three_prime_UTR 60.0%
! GAGTGCGTTGGCGCTTCAGA+TGG - chr3_1:54795527-54795546 Msa0362940:three_prime_UTR 60.0%
TGCTCGCTCGTTGCACGAAG+AGG + chr3_1:54796314-54796333 None:intergenic 60.0%
GGGCCTGCGAGTCACTTCTT+TGG - chr3_1:54797014-54797033 Msa0362940:CDS 60.0%
! TAGAGTCCATGGCGGAGGTG+TGG + chr3_1:54797189-54797208 None:intergenic 60.0%
AGAGTGGGGACTGGAGACAG+AGG + chr3_1:54797219-54797238 None:intergenic 60.0%
!! GAGCTCACTCAGTGTTGGCG+TGG + chr3_1:54797244-54797263 None:intergenic 60.0%
AACAGGCGGTTGGGAGGGAG+AGG + chr3_1:54797143-54797162 None:intergenic 65.0%
! AGGTGTGGTGGCGACTGGCT+TGG + chr3_1:54797174-54797193 None:intergenic 65.0%
! AGTCCATGGCGGAGGTGTGG+TGG + chr3_1:54797186-54797205 None:intergenic 65.0%
GGCGTGGTTAGAGTGGGGAC+TGG + chr3_1:54797228-54797247 None:intergenic 65.0%
TCGCCACCACACCTCCGCCA+TGG - chr3_1:54797180-54797199 Msa0362940:CDS 70.0%
! GGCGGAGGTGTGGTGGCGAC+TGG + chr3_1:54797179-54797198 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 54795315 54797813 54795315 ID=Msa0362940;Name=Msa0362940
chr3_1 mRNA 54795315 54797813 54795315 ID=Msa0362940-mRNA-1;Parent=Msa0362940;Name=Msa0362940-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0.5|1|0|0|2|311|350
chr3_1 exon 54795315 54795724 54795315 ID=Msa0362940-mRNA-1:exon:9952;Parent=Msa0362940-mRNA-1
chr3_1 exon 54796860 54797813 54796860 ID=Msa0362940-mRNA-1:exon:9951;Parent=Msa0362940-mRNA-1
chr3_1 CDS 54796860 54797813 54796860 ID=Msa0362940-mRNA-1:cds;Parent=Msa0362940-mRNA-1
chr3_1 CDS 54795626 54795724 54795626 ID=Msa0362940-mRNA-1:cds;Parent=Msa0362940-mRNA-1
chr3_1 three_prime_UTR 54795315 54795625 54795315 ID=Msa0362940-mRNA-1:three_prime_utr;Parent=Msa0362940-mRNA-1
Gene Sequence

>Msa0362940

ATGGGCGAACATGGTGTTGCACGTTCTTTGTTAAGGTTGTTGCCTCGTGCCGAGGGAAAAATGCAAAAAGTGTTGATAGAATGTTTGTTGGTTGTTGTAAGTTTTTGTGATAATAGTAGAACATTTGTTGCAACTAACGGTGGTTTAGAGATTATTATTAGTTTGTTGAGTAGTTGTAGCGATGATGTTAGATTGTATTTGTTAGAGATTTTGAGTGCGTTGGCGCTTCAGATGGAAGTTAGGAGGGAGCTCATTAGATTAGGAGCACTTTGTTTTATTGTTGAGGCTGCTGGTGTTGGTAACATGATTTCGAGGGAAAGGGCGTGTCAATCGATTGGATTGGTTGGTCTTTCAAGACAAGGTAACGTGCTTGTGGAGTTGTTTAGTGATGGAGATAACACTACAAAACTTGTGGCCGGTAATACTCTTGGTGTGCTATTGAACCATGTTGATTACATCAGACCAATTGCTCAAGCTGGGGCTATACCTCTTTATGCGGAGCTTCTTCAAGGAGATGATGTTTCTGGTAAGGAGATAGCCGAGGATGTGTTTTGTATATTGGCTGTTGCAGAGGCGAATGCTGTTGAAATTGTGGGTCATTTGGTGAGACTTCTTAGAGAAGGTGATGACGAAGCTAAAGCAGCAGCTGTCGATGTGATATGGGATCTGTTGCGATACAAGCACACACCTTCTGTGATCCGGAACTCAGGTGCGGCTTTTATCCTTGTTGAGTTGTTGAGCCATGGGACTGAAGAAATAAAGAAAAAGATTTCTGGGGCTTTTTCTCAGATGAGCTACCAGGAAAGTGGTAGAATGGTCCTTGGTGATGCAAGAGCAATTCCGGTTCTTATTGATTTACTACATGACGAGACTGGGGAACTAAGAAATAATGTGGTTGAGGCTCTTTCTAATTTTCATGAGGATCCATTGTATCATGATATATTATCTGATGTAAATAGACTAATTCGTATTCATGCATCGAATGGGCATATGCCTAGATCTTTATGTAGAACGAGTGTTGAGCAACTCATTTGGATCCTGGATCATGTTTAA
Protein sequence

>Msa0362940

MGEHGVARSLLRLLPRAEGKMQKVLIECLLVVVSFCDNSRTFVATNGGLEIIISLLSSCSDDVRLYLLEILSALALQMEVRRELIRLGALCFIVEAAGVGNMISRERACQSIGLVGLSRQGNVLVELFSDGDNTTKLVAGNTLGVLLNHVDYIRPIAQAGAIPLYAELLQGDDVSGKEIAEDVFCILAVAEANAVEIVGHLVRLLREGDDEAKAAAVDVIWDLLRYKHTPSVIRNSGAAFILVELLSHGTEEIKKKISGAFSQMSYQESGRMVLGDARAIPVLIDLLHDETGELRNNVVEALSNFHEDPLYHDILSDVNRLIRIHASNGHMPRSLCRTSVEQLIWILDHV*