AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1225940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1225940 MtrunA17_Chr3g0101311 85.042 361 34 2 1 341 100 460 0.0 600
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1225940 AT3G20170.1 48.919 370 159 5 1 343 102 468 2.31e-104 315

Find 89 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAAGGAGATGATGTTTC+TGG 0.181044 8_1:-91618726 Msa1225940:CDS
ATCATGTTTAATTATCTTAC+AGG 0.218306 8_1:-91617053 Msa1225940:exon
CAGCAGCTGTCGATGTGATA+TGG 0.278624 8_1:-91618590 Msa1225940:CDS
TGGTGATGCAAGAGCAATTC+CGG 0.352200 8_1:-91618409 Msa1225940:CDS
GGACCATTCTACCACTTTCC+TGG 0.363654 8_1:+91618433 None:intergenic
TTACAACATACATCTATGAT+AGG 0.385514 8_1:+91616963 None:intergenic
CGAGTGTTGAGCAACTCATT+TGG 0.385896 8_1:-91617083 Msa1225940:CDS
TGGCGCTTCAGATGGAAGTT+AGG 0.404983 8_1:-91619010 Msa1225940:CDS
GATTTACTACATGACGAGAC+TGG 0.405844 8_1:-91618378 Msa1225940:CDS
ATAGCCCCAGCTTGAGCAAT+TGG 0.406485 8_1:+91618768 None:intergenic
GTATATAGTCATTTGCTTGT+AGG 0.407308 8_1:-91616884 Msa1225940:three_prime_UTR
ATGTATGTTGTAATGAAAAT+TGG 0.409597 8_1:-91616953 Msa1225940:three_prime_UTR
TAAAAGCCGCACCTGAGTTC+CGG 0.410207 8_1:+91618532 None:intergenic
AGGAGGGAGCTCATTAGATT+AGG 0.412597 8_1:-91618990 Msa1225940:CDS
TAATTATAAGTTACAACAAT+AGG 0.413182 8_1:+91616823 None:intergenic
GTTGTATAATTTATAAAAGA+GGG 0.413839 8_1:-91616908 Msa1225940:three_prime_UTR
TCTTTATTTCTTCAGTCCCA+TGG 0.430621 8_1:+91618490 None:intergenic
TAGCACACCAAGAGTATTAC+CGG 0.430738 8_1:+91618815 None:intergenic
ATCAGACCAATTGCTCAAGC+TGG 0.432583 8_1:-91618774 Msa1225940:CDS
CTTTATGCGGAGCTTCTTCA+AGG 0.433825 8_1:-91618741 Msa1225940:CDS
TGTTGAAATTGTGGGTCATT+TGG 0.446283 8_1:-91618649 Msa1225940:CDS
GTGTTGCACGTTCTTTGTTA+AGG 0.459610 8_1:-91619217 Msa1225940:CDS
TGTTGTATAATTTATAAAAG+AGG 0.462397 8_1:-91616909 Msa1225940:three_prime_UTR
AGTGTTGATAGAATGTTTGT+TGG 0.463303 8_1:-91619162 Msa1225940:CDS
TGAGCAACTCATTTGGATCC+TGG 0.466205 8_1:-91617076 Msa1225940:CDS
TGTAGTAAATCAATAAGAAC+CGG 0.466912 8_1:+91618390 None:intergenic
CTTTGAACCTCGATTAAACC+TGG 0.467265 8_1:+91616746 None:intergenic
AGCAGCTGTCGATGTGATAT+GGG 0.479152 8_1:-91618589 Msa1225940:CDS
CTTGTTGAGTTGTTGAGCCA+TGG 0.489304 8_1:-91618507 Msa1225940:CDS
AGGAGATGATGTTTCTGGTA+AGG 0.494027 8_1:-91618721 Msa1225940:CDS
CGCTTCAGATGGAAGTTAGG+AGG 0.496594 8_1:-91619007 Msa1225940:CDS
ATTGTTGAGGCTGCTGGTGT+TGG 0.498526 8_1:-91618954 Msa1225940:CDS
AGCACACACCTTCTGTGATC+CGG 0.498591 8_1:-91618551 Msa1225940:CDS
ATTTACTACATGACGAGACT+GGG 0.505397 8_1:-91618377 Msa1225940:CDS
AAGGTTGTTGCCTCGTGCCG+AGG 0.506613 8_1:-91619198 Msa1225940:CDS
TTCGTATTCATGCATCGAAT+GGG 0.509754 8_1:-91617131 Msa1225940:CDS
TAACATGATTTCGAGGGAAA+GGG 0.510380 8_1:-91618931 Msa1225940:CDS
ACTAAGAAATAATGTGGTTG+AGG 0.515775 8_1:-91618352 Msa1225940:CDS
TGAAGAAGCTCCGCATAAAG+AGG 0.516045 8_1:+91618744 None:intergenic
TCAGACCAATTGCTCAAGCT+GGG 0.516253 8_1:-91618773 Msa1225940:CDS
TTGTTGAGTTGTTGAGCCAT+GGG 0.519539 8_1:-91618506 Msa1225940:CDS
GAGTGCGTTGGCGCTTCAGA+TGG 0.520748 8_1:-91619018 Msa1225940:CDS
AAGATAATTAAACATGATCC+AGG 0.523474 8_1:+91617058 None:intergenic
CTTGTGGAGTTGTTTAGTGA+TGG 0.523872 8_1:-91618861 Msa1225940:CDS
GTAACATGATTTCGAGGGAA+AGG 0.527617 8_1:-91618932 Msa1225940:CDS
GAAAGGGCGTGTCAATCGAT+TGG 0.529311 8_1:-91618915 Msa1225940:CDS
ATTCGTATTCATGCATCGAA+TGG 0.531293 8_1:-91617132 Msa1225940:CDS
TGTCAATCGATTGGATTGGT+TGG 0.533723 8_1:-91618906 Msa1225940:CDS
AAGACAAGGTAACGTGCTTG+TGG 0.535072 8_1:-91618877 Msa1225940:CDS
CTTGTGGCCGGTAATACTCT+TGG 0.540316 8_1:-91618822 Msa1225940:CDS
CCTTCTGTGATCCGGAACTC+AGG 0.545760 8_1:-91618543 Msa1225940:CDS
CTACCAGGAAAGTGGTAGAA+TGG 0.552394 8_1:-91618436 Msa1225940:CDS
GGCGAATGCTGTTGAAATTG+TGG 0.555096 8_1:-91618658 Msa1225940:CDS
AAAATTGGTAGAAACAGTCT+TGG 0.557659 8_1:-91616938 Msa1225940:three_prime_UTR
CTCGTTCTACATAAAGATCT+AGG 0.558292 8_1:+91617102 None:intergenic
TTGGTGAGACTTCTTAGAGA+AGG 0.565763 8_1:-91618630 Msa1225940:CDS
AGAACATTTGTTGCAACTAA+CGG 0.565795 8_1:-91619113 Msa1225940:CDS
GTGTTGGTAACATGATTTCG+AGG 0.566458 8_1:-91618938 Msa1225940:CDS
ACTTATAATTATTACTGATG+AGG 0.567458 8_1:-91616811 Msa1225940:three_prime_UTR
TACAACATACATCTATGATA+GGG 0.569446 8_1:+91616964 None:intergenic
GCGAATGCTGTTGAAATTGT+GGG 0.576159 8_1:-91618657 Msa1225940:CDS
GAAAGTGGTAGAATGGTCCT+TGG 0.580251 8_1:-91618429 Msa1225940:CDS
TTGTATATTGGCTGTTGCAG+AGG 0.581097 8_1:-91618679 Msa1225940:CDS
AGGTTGTTGCCTCGTGCCGA+GGG 0.584981 8_1:-91619197 Msa1225940:CDS
CAGATGAGCTACCAGGAAAG+TGG 0.586307 8_1:-91618444 Msa1225940:CDS
AACACTACAAAACTTGTGGC+CGG 0.587605 8_1:-91618834 Msa1225940:CDS
GCTATTGAAATGGGCGAACA+TGG 0.595334 8_1:-91619239 None:intergenic
TTGGTTGGTCTTTCAAGACA+AGG 0.607223 8_1:-91618891 Msa1225940:CDS
GGCGTGTCAATCGATTGGAT+TGG 0.609217 8_1:-91618910 Msa1225940:CDS
GATAATATATCATGATACAA+TGG 0.610806 8_1:+91618306 None:intergenic
CAATATACAAAACACATCCT+CGG 0.611174 8_1:+91618692 None:intergenic
CCTGAGTTCCGGATCACAGA+AGG 0.612592 8_1:+91618543 None:intergenic
TGTTGGTAACATGATTTCGA+GGG 0.613350 8_1:-91618937 Msa1225940:CDS
GCTTCAGATGGAAGTTAGGA+GGG 0.632267 8_1:-91619006 Msa1225940:CDS
TGGGGAACTAAGAAATAATG+TGG 0.637165 8_1:-91618358 Msa1225940:CDS
TCGATGAATCAGAAACAACC+AGG 0.638423 8_1:-91616764 Msa1225940:three_prime_UTR
CATGGCTCAACAACTCAACA+AGG 0.647415 8_1:+91618508 None:intergenic
GAATTGCTCTTGCATCACCA+AGG 0.647653 8_1:+91618412 None:intergenic
AGATAACACTACAAAACTTG+TGG 0.650577 8_1:-91618838 Msa1225940:CDS
TAGGGAGTGAAACTGATACA+AGG 0.660556 8_1:+91616982 None:intergenic
ACATTTGTTGCAACTAACGG+TGG 0.663331 8_1:-91619110 Msa1225940:CDS
TGGGGCTATACCTCTTTATG+CGG 0.668733 8_1:-91618754 Msa1225940:CDS
GAAACAACCAGGTTTAATCG+AGG 0.672561 8_1:-91616753 None:intergenic
CAGACCAATTGCTCAAGCTG+GGG 0.674341 8_1:-91618772 Msa1225940:CDS
TTGGTCTGATGTAATCAACA+TGG 0.683529 8_1:+91618787 None:intergenic
TTTACTACATGACGAGACTG+GGG 0.685708 8_1:-91618376 Msa1225940:CDS
ATTCATCGAGAATAACACAA+AGG 0.688249 8_1:+91616778 None:intergenic
TTCTGGTAAGGAGATAGCCG+AGG 0.692171 8_1:-91618709 Msa1225940:CDS
TGTGATCCGGAACTCAGGTG+CGG 0.706175 8_1:-91618538 Msa1225940:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAATACAAAATGATTT+GGG + chr8_1:91618074-91618093 None:intergenic 10.0%
!! TTAAATAATACAAAATGATT+TGG + chr8_1:91618075-91618094 None:intergenic 10.0%
!!! TTTTTATTGATTTTGATAAA+GGG + chr8_1:91618519-91618538 None:intergenic 10.0%
!! AAATAAAGAAAAAGATTTCT+GGG - chr8_1:91617508-91617527 Msa1225940:intron 15.0%
!!! AAAATATTGTTACTTTCTTA+AGG + chr8_1:91618220-91618239 None:intergenic 15.0%
!!! TTTTATTGATTTTGATAAAG+GGG + chr8_1:91618518-91618537 None:intergenic 15.0%
!!! CTTTTTATTGATTTTGATAA+AGG + chr8_1:91618520-91618539 None:intergenic 15.0%
!! TGTTGTATAATTTATAAAAG+AGG - chr8_1:91619075-91619094 Msa1225940:CDS 15.0%
!! GTTGTATAATTTATAAAAGA+GGG - chr8_1:91619076-91619095 Msa1225940:CDS 15.0%
!! TAATTATAAGTTACAACAAT+AGG + chr8_1:91619164-91619183 None:intergenic 15.0%
!! GAAATAAAGAAAAAGATTTC+TGG - chr8_1:91617507-91617526 Msa1225940:intron 20.0%
!! AATAAAGAAAAAGATTTCTG+GGG - chr8_1:91617509-91617528 Msa1225940:intron 20.0%
!! GATAATATATCATGATACAA+TGG + chr8_1:91617681-91617700 None:intergenic 20.0%
!! AGATTGATTAAACATGATTT+GGG + chr8_1:91617824-91617843 None:intergenic 20.0%
!! AAGATTGATTAAACATGATT+TGG + chr8_1:91617825-91617844 None:intergenic 20.0%
!!! ATTTTCTTCTTATAAGTGTA+GGG - chr8_1:91618433-91618452 Msa1225940:CDS 20.0%
!!! TTTATTGATTTTGATAAAGG+GGG + chr8_1:91618517-91618536 None:intergenic 20.0%
!! ATCTCTTCATATTCTTTATA+CGG + chr8_1:91618548-91618567 None:intergenic 20.0%
!! ATCATGTTTAATTATCTTAC+AGG - chr8_1:91618931-91618950 Msa1225940:CDS 20.0%
!! ATGTATGTTGTAATGAAAAT+TGG - chr8_1:91619031-91619050 Msa1225940:CDS 20.0%
!! ACTTATAATTATTACTGATG+AGG - chr8_1:91619173-91619192 Msa1225940:CDS 20.0%
! TGTAGTAAATCAATAAGAAC+CGG + chr8_1:91617597-91617616 None:intergenic 25.0%
! TCATGTTTAATCAATCTTAC+AGG - chr8_1:91617827-91617846 Msa1225940:intron 25.0%
! TTGATCTTTACATAGATTTC+CGG - chr8_1:91617856-91617875 Msa1225940:intron 25.0%
!! ATATATACTCGTTTGTTAAC+CGG + chr8_1:91617878-91617897 None:intergenic 25.0%
!! AGCATTTTCTTACAAGTAAA+TGG + chr8_1:91617901-91617920 None:intergenic 25.0%
! GAGGTGACAAATAATAAAAT+TGG + chr8_1:91618011-91618030 None:intergenic 25.0%
! CTACACTTATAAGAAGAAAA+TGG + chr8_1:91618434-91618453 None:intergenic 25.0%
!! CATTTTCTTCTTATAAGTGT+AGG - chr8_1:91618432-91618451 Msa1225940:CDS 25.0%
!! ACTAAGAAATATTGTTGTTG+AGG - chr8_1:91618739-91618758 Msa1225940:CDS 25.0%
! TAAAGGATCTTCATGAAAAT+TGG + chr8_1:91618771-91618790 None:intergenic 25.0%
! AAGATAATTAAACATGATCC+AGG + chr8_1:91618929-91618948 None:intergenic 25.0%
! TACAACATACATCTATGATA+GGG + chr8_1:91619023-91619042 None:intergenic 25.0%
! TTACAACATACATCTATGAT+AGG + chr8_1:91619024-91619043 None:intergenic 25.0%
!! AGTGTTGATAGAATGTTTGT+TGG - chr8_1:91616822-91616841 Msa1225940:three_prime_UTR 30.0%
! AGAACATTTGTTGCAACTAA+CGG - chr8_1:91616871-91616890 Msa1225940:three_prime_UTR 30.0%
!!! AGCACTTTGTTTTATTGTTG+AGG - chr8_1:91617017-91617036 Msa1225940:three_prime_UTR 30.0%
AGATAACACTACAAAACTTG+TGG - chr8_1:91617146-91617165 Msa1225940:intron 30.0%
CAATATACAAAACACATCCT+CGG + chr8_1:91617295-91617314 None:intergenic 30.0%
ACTAAGAAATAATGTGGTTG+AGG - chr8_1:91617632-91617651 Msa1225940:intron 30.0%
TTCTGCAAAATTCTGAATGA+AGG + chr8_1:91617720-91617739 None:intergenic 30.0%
ATTACTTTCCTCATCAGTAA+TGG + chr8_1:91617955-91617974 None:intergenic 30.0%
! TAATACAAAATGATTTGGGC+AGG + chr8_1:91618070-91618089 None:intergenic 30.0%
AATGGGATTGTAGAACTTTA+AGG + chr8_1:91618147-91618166 None:intergenic 30.0%
TTAAAGTTCTACAATCCCAT+TGG - chr8_1:91618146-91618165 Msa1225940:intron 30.0%
TAAAGTTCTACAATCCCATT+GGG - chr8_1:91618147-91618166 Msa1225940:intron 30.0%
TGTTAAACAGAGTATGACAA+CGG + chr8_1:91618185-91618204 None:intergenic 30.0%
! GACTATGGTTTAGAATTAGA+CGG + chr8_1:91618405-91618424 None:intergenic 30.0%
ATATTTCTTAGTTCACTCAG+TGG + chr8_1:91618731-91618750 None:intergenic 30.0%
CAGATAATCAATCAGTGTAA+AGG + chr8_1:91618788-91618807 None:intergenic 30.0%
TTCTGCATATATCAGAATGA+AGG + chr8_1:91618825-91618844 None:intergenic 30.0%
AAAATTGGTAGAAACAGTCT+TGG - chr8_1:91619046-91619065 Msa1225940:CDS 30.0%
GTATATAGTCATTTGCTTGT+AGG - chr8_1:91619100-91619119 Msa1225940:CDS 30.0%
ATTCATCGAGAATAACACAA+AGG + chr8_1:91619209-91619228 None:intergenic 30.0%
!! CACTTTTTGCATTTTTCCCT+CGG + chr8_1:91616806-91616825 None:intergenic 35.0%
!! TGTTGGTAACATGATTTCGA+GGG - chr8_1:91617047-91617066 Msa1225940:exon 35.0%
TAACATGATTTCGAGGGAAA+GGG - chr8_1:91617053-91617072 Msa1225940:exon 35.0%
TTGGTCTGATGTAATCAACA+TGG + chr8_1:91617200-91617219 None:intergenic 35.0%
! CGAGGATGTGTTTTGTATAT+TGG - chr8_1:91617293-91617312 Msa1225940:intron 35.0%
TGTTGAAATTGTGGGTCATT+TGG - chr8_1:91617335-91617354 Msa1225940:intron 35.0%
! TCTTTATTTCTTCAGTCCCA+TGG + chr8_1:91617497-91617516 None:intergenic 35.0%
ATTTACTACATGACGAGACT+GGG - chr8_1:91617607-91617626 Msa1225940:intron 35.0%
TGGGGAACTAAGAAATAATG+TGG - chr8_1:91617626-91617645 Msa1225940:intron 35.0%
! GGCTCTTTCTAATTTTCATG+AGG - chr8_1:91617653-91617672 Msa1225940:intron 35.0%
TATGACAACGGTTGAACTAA+AGG + chr8_1:91618173-91618192 None:intergenic 35.0%
TCTGTTTAACAGAGAGCTTA+TGG - chr8_1:91618194-91618213 Msa1225940:intron 35.0%
AGAAAATGGAGTAGTGACTA+TGG + chr8_1:91618420-91618439 None:intergenic 35.0%
AAATGTGTGTCGCAAAATGT+TGG + chr8_1:91618482-91618501 None:intergenic 35.0%
! TTTTGCGACACACATTTTGA+CGG - chr8_1:91618485-91618504 Msa1225940:CDS 35.0%
ATTCGTATTCATGCATCGAA+TGG - chr8_1:91618852-91618871 Msa1225940:CDS 35.0%
TTCGTATTCATGCATCGAAT+GGG - chr8_1:91618853-91618872 Msa1225940:CDS 35.0%
CTCGTTCTACATAAAGATCT+AGG + chr8_1:91618885-91618904 None:intergenic 35.0%
! CTTTTACAAAGCAGGTTGTT+GGG - chr8_1:91618972-91618991 Msa1225940:CDS 35.0%
GTGTTGCACGTTCTTTGTTA+AGG - chr8_1:91616767-91616786 Msa1225940:three_prime_UTR 40.0%
! ACATTTGTTGCAACTAACGG+TGG - chr8_1:91616874-91616893 Msa1225940:three_prime_UTR 40.0%
!! GTTAGAGATTTTGAGTGCGT+TGG - chr8_1:91616954-91616973 Msa1225940:three_prime_UTR 40.0%
!! TGTTTTATTGTTGAGGCTGC+TGG - chr8_1:91617024-91617043 Msa1225940:three_prime_UTR 40.0%
!! GTGTTGGTAACATGATTTCG+AGG - chr8_1:91617046-91617065 Msa1225940:exon 40.0%
GTAACATGATTTCGAGGGAA+AGG - chr8_1:91617052-91617071 Msa1225940:exon 40.0%
!! TGTCAATCGATTGGATTGGT+TGG - chr8_1:91617078-91617097 Msa1225940:CDS 40.0%
!! TTGGTTGGTCTTTCAAGACA+AGG - chr8_1:91617093-91617112 Msa1225940:CDS 40.0%
CTTGTGGAGTTGTTTAGTGA+TGG - chr8_1:91617123-91617142 Msa1225940:CDS 40.0%
AACACTACAAAACTTGTGGC+CGG - chr8_1:91617150-91617169 Msa1225940:intron 40.0%
!! TAGCACACCAAGAGTATTAC+CGG + chr8_1:91617172-91617191 None:intergenic 40.0%
CTTCAAGGAGATGATGTTTC+TGG - chr8_1:91617258-91617277 Msa1225940:intron 40.0%
!! AGGAGATGATGTTTCTGGTA+AGG - chr8_1:91617263-91617282 Msa1225940:intron 40.0%
! TTGTATATTGGCTGTTGCAG+AGG - chr8_1:91617305-91617324 Msa1225940:intron 40.0%
GCGAATGCTGTTGAAATTGT+GGG - chr8_1:91617327-91617346 Msa1225940:intron 40.0%
TTGGTGAGACTTCTTAGAGA+AGG - chr8_1:91617354-91617373 Msa1225940:intron 40.0%
! TTGTTGAGTTGTTGAGCCAT+GGG - chr8_1:91617478-91617497 Msa1225940:intron 40.0%
! TTTTTCTCAGATGAGCTACC+AGG - chr8_1:91617533-91617552 Msa1225940:intron 40.0%
GATTTACTACATGACGAGAC+TGG - chr8_1:91617606-91617625 Msa1225940:intron 40.0%
TTTACTACATGACGAGACTG+GGG - chr8_1:91617608-91617627 Msa1225940:intron 40.0%
CGATTATCGAGTAGCTCATT+TGG - chr8_1:91617796-91617815 Msa1225940:intron 40.0%
AAATGCCACCATTACTGATG+AGG - chr8_1:91617944-91617963 Msa1225940:intron 40.0%
ACTTTCCTCATCAGTAATGG+TGG + chr8_1:91617952-91617971 None:intergenic 40.0%
GACTCATCGGGAATAATACA+AGG + chr8_1:91617980-91617999 None:intergenic 40.0%
TATTCCCGATGAGTCAGAAT+CGG - chr8_1:91617985-91618004 Msa1225940:intron 40.0%
TTTGTCACCTCATTTCAGTG+AGG - chr8_1:91618020-91618039 Msa1225940:intron 40.0%
GTCCAAGGAGAATTGCATTT+CGG - chr8_1:91618042-91618061 Msa1225940:intron 40.0%
AAATTGAGCTCACTCAGTGT+TGG + chr8_1:91618688-91618707 None:intergenic 40.0%
! TCTTGACGCTTTTACAAAGC+AGG - chr8_1:91618964-91618983 Msa1225940:CDS 40.0%
! GCTTTTACAAAGCAGGTTGT+TGG - chr8_1:91618971-91618990 Msa1225940:CDS 40.0%
TAGGGAGTGAAACTGATACA+AGG + chr8_1:91619005-91619024 None:intergenic 40.0%
TCGATGAATCAGAAACAACC+AGG - chr8_1:91619220-91619239 Msa1225940:CDS 40.0%
GCTTCAGATGGAAGTTAGGA+GGG - chr8_1:91616978-91616997 Msa1225940:three_prime_UTR 45.0%
AGGAGGGAGCTCATTAGATT+AGG - chr8_1:91616994-91617013 Msa1225940:three_prime_UTR 45.0%
AAGACAAGGTAACGTGCTTG+TGG - chr8_1:91617107-91617126 Msa1225940:CDS 45.0%
ATCAGACCAATTGCTCAAGC+TGG - chr8_1:91617210-91617229 Msa1225940:intron 45.0%
TCAGACCAATTGCTCAAGCT+GGG - chr8_1:91617211-91617230 Msa1225940:intron 45.0%
TGGGGCTATACCTCTTTATG+CGG - chr8_1:91617230-91617249 Msa1225940:intron 45.0%
TGAAGAAGCTCCGCATAAAG+AGG + chr8_1:91617243-91617262 None:intergenic 45.0%
CTTTATGCGGAGCTTCTTCA+AGG - chr8_1:91617243-91617262 Msa1225940:intron 45.0%
GGCGAATGCTGTTGAAATTG+TGG - chr8_1:91617326-91617345 Msa1225940:intron 45.0%
AGCAGCTGTCGATGTGATAT+GGG - chr8_1:91617395-91617414 Msa1225940:intron 45.0%
CATGGCTCAACAACTCAACA+AGG + chr8_1:91617479-91617498 None:intergenic 45.0%
! CTTGTTGAGTTGTTGAGCCA+TGG - chr8_1:91617477-91617496 Msa1225940:intron 45.0%
CTACCAGGAAAGTGGTAGAA+TGG - chr8_1:91617548-91617567 Msa1225940:intron 45.0%
GAAAGTGGTAGAATGGTCCT+TGG - chr8_1:91617555-91617574 Msa1225940:intron 45.0%
GAATTGCTCTTGCATCACCA+AGG + chr8_1:91617575-91617594 None:intergenic 45.0%
! TGGTGATGCAAGAGCAATTC+CGG - chr8_1:91617575-91617594 Msa1225940:intron 45.0%
! ATTGGCCGATTCTGACTCAT+CGG + chr8_1:91617993-91618012 None:intergenic 45.0%
AGCCGAAATGCAATTCTCCT+TGG + chr8_1:91618047-91618066 None:intergenic 45.0%
GGTTGAACTAAAGGCCCAAT+GGG + chr8_1:91618164-91618183 None:intergenic 45.0%
TCTTAGTTCACTCAGTGGAG+AGG + chr8_1:91618726-91618745 None:intergenic 45.0%
!! CGAGTGTTGAGCAACTCATT+TGG - chr8_1:91618901-91618920 Msa1225940:CDS 45.0%
TGAGCAACTCATTTGGATCC+TGG - chr8_1:91618908-91618927 Msa1225940:CDS 45.0%
TGGCGCTTCAGATGGAAGTT+AGG - chr8_1:91616974-91616993 Msa1225940:three_prime_UTR 50.0%
CGCTTCAGATGGAAGTTAGG+AGG - chr8_1:91616977-91616996 Msa1225940:three_prime_UTR 50.0%
ATTGTTGAGGCTGCTGGTGT+TGG - chr8_1:91617030-91617049 Msa1225940:three_prime_UTR 50.0%
! GAAAGGGCGTGTCAATCGAT+TGG - chr8_1:91617069-91617088 Msa1225940:CDS 50.0%
GGCGTGTCAATCGATTGGAT+TGG - chr8_1:91617074-91617093 Msa1225940:CDS 50.0%
CTTGTGGCCGGTAATACTCT+TGG - chr8_1:91617162-91617181 Msa1225940:intron 50.0%
CAGACCAATTGCTCAAGCTG+GGG - chr8_1:91617212-91617231 Msa1225940:intron 50.0%
ATAGCCCCAGCTTGAGCAAT+TGG + chr8_1:91617219-91617238 None:intergenic 50.0%
TTCTGGTAAGGAGATAGCCG+AGG - chr8_1:91617275-91617294 Msa1225940:intron 50.0%
CAGCAGCTGTCGATGTGATA+TGG - chr8_1:91617394-91617413 Msa1225940:intron 50.0%
AGCACACACCTTCTGTGATC+CGG - chr8_1:91617433-91617452 Msa1225940:intron 50.0%
TAAAAGCCGCACCTGAGTTC+CGG + chr8_1:91617455-91617474 None:intergenic 50.0%
CAGATGAGCTACCAGGAAAG+TGG - chr8_1:91617540-91617559 Msa1225940:intron 50.0%
GGACCATTCTACCACTTTCC+TGG + chr8_1:91617554-91617573 None:intergenic 50.0%
! TTGGCCGATTCTGACTCATC+GGG + chr8_1:91617992-91618011 None:intergenic 50.0%
CCTTGGACCTCACTGAAATG+AGG + chr8_1:91618030-91618049 None:intergenic 50.0%
CCTCATTTCAGTGAGGTCCA+AGG - chr8_1:91618027-91618046 Msa1225940:intron 50.0%
CGGTTGAACTAAAGGCCCAA+TGG + chr8_1:91618165-91618184 None:intergenic 50.0%
TAGACGGTGCTTAGTGCGAA+AGG + chr8_1:91618389-91618408 None:intergenic 50.0%
AGACCAAAGAAGTGACTCGC+AGG + chr8_1:91618459-91618478 None:intergenic 50.0%
GACAACAACAACAGGCGGTT+GGG + chr8_1:91618591-91618610 None:intergenic 50.0%
GCTTGGACAACAACAACAGG+CGG + chr8_1:91618596-91618615 None:intergenic 50.0%
!! CTGGCTTGGACAACAACAAC+AGG + chr8_1:91618599-91618618 None:intergenic 50.0%
GCTGTTGTTAGAGTCCATGG+CGG + chr8_1:91618636-91618655 None:intergenic 50.0%
GAGGCTGTTGTTAGAGTCCA+TGG + chr8_1:91618639-91618658 None:intergenic 50.0%
!! AGTGTTGGCGTGGTTAGAGT+GGG + chr8_1:91618673-91618692 None:intergenic 50.0%
! TGCATTTTTCCCTCGGCACG+AGG + chr8_1:91616799-91616818 None:intergenic 55.0%
CCTGAGTTCCGGATCACAGA+AGG + chr8_1:91617444-91617463 None:intergenic 55.0%
CCTTCTGTGATCCGGAACTC+AGG - chr8_1:91617441-91617460 Msa1225940:intron 55.0%
TGTGATCCGGAACTCAGGTG+CGG - chr8_1:91617446-91617465 Msa1225940:intron 55.0%
ACAACAACAGGCGGTTGGGA+GGG + chr8_1:91618587-91618606 None:intergenic 55.0%
AACAACAACAGGCGGTTGGG+AGG + chr8_1:91618588-91618607 None:intergenic 55.0%
GGACAACAACAACAGGCGGT+TGG + chr8_1:91618592-91618611 None:intergenic 55.0%
GTTGTTAGAGTCCATGGCGG+AGG + chr8_1:91618633-91618652 None:intergenic 55.0%
! GTGTTGGCGTGGTTAGAGTG+GGG + chr8_1:91618672-91618691 None:intergenic 55.0%
!! CAGTGTTGGCGTGGTTAGAG+TGG + chr8_1:91618674-91618693 None:intergenic 55.0%
AAGGTTGTTGCCTCGTGCCG+AGG - chr8_1:91616786-91616805 Msa1225940:three_prime_UTR 60.0%
AGGTTGTTGCCTCGTGCCGA+GGG - chr8_1:91616787-91616806 Msa1225940:three_prime_UTR 60.0%
! GAGTGCGTTGGCGCTTCAGA+TGG - chr8_1:91616966-91616985 Msa1225940:three_prime_UTR 60.0%
TGCTCGCTCGTTGCACGAAG+AGG + chr8_1:91617753-91617772 None:intergenic 60.0%
GGGCCTGCGAGTCACTTCTT+TGG - chr8_1:91618453-91618472 Msa1225940:CDS 60.0%
! TAGAGTCCATGGCGGAGGTG+TGG + chr8_1:91618628-91618647 None:intergenic 60.0%
AGAGTGGGGACTGGAGACAG+AGG + chr8_1:91618658-91618677 None:intergenic 60.0%
!! GAGCTCACTCAGTGTTGGCG+TGG + chr8_1:91618683-91618702 None:intergenic 60.0%
AACAGGCGGTTGGGAGGGAG+AGG + chr8_1:91618582-91618601 None:intergenic 65.0%
! AGGTGTGGTGGCGACTGGCT+TGG + chr8_1:91618613-91618632 None:intergenic 65.0%
! AGTCCATGGCGGAGGTGTGG+TGG + chr8_1:91618625-91618644 None:intergenic 65.0%
GGCGTGGTTAGAGTGGGGAC+TGG + chr8_1:91618667-91618686 None:intergenic 65.0%
TCGCCACCACACCTCCGCCA+TGG - chr8_1:91618619-91618638 Msa1225940:CDS 70.0%
! GGCGGAGGTGTGGTGGCGAC+TGG + chr8_1:91618618-91618637 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 91616754 91619252 91616754 ID=Msa1225940;Name=Msa1225940
chr8_1 mRNA 91616754 91619252 91616754 ID=Msa1225940-mRNA-1;Parent=Msa1225940;Name=Msa1225940-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0.5|1|0|0|2|311|350
chr8_1 exon 91616754 91617163 91616754 ID=Msa1225940-mRNA-1:exon:33403;Parent=Msa1225940-mRNA-1
chr8_1 exon 91618299 91619252 91618299 ID=Msa1225940-mRNA-1:exon:33402;Parent=Msa1225940-mRNA-1
chr8_1 CDS 91618299 91619252 91618299 ID=Msa1225940-mRNA-1:cds;Parent=Msa1225940-mRNA-1
chr8_1 CDS 91617065 91617163 91617065 ID=Msa1225940-mRNA-1:cds;Parent=Msa1225940-mRNA-1
chr8_1 three_prime_UTR 91616754 91617064 91616754 ID=Msa1225940-mRNA-1:three_prime_utr;Parent=Msa1225940-mRNA-1
Gene Sequence

>Msa1225940

ATGGGCGAACATGGTGTTGCACGTTCTTTGTTAAGGTTGTTGCCTCGTGCCGAGGGAAAAATGCAAAAAGTGTTGATAGAATGTTTGTTGGTTGTTGTAAGTTTTTGTGATAATAGTAGAACATTTGTTGCAACTAACGGTGGTTTAGAGATTATTATTAGTTTGTTGAGTAGTTGTAGCGATGATGTTAGATTGTATTTGTTAGAGATTTTGAGTGCGTTGGCGCTTCAGATGGAAGTTAGGAGGGAGCTCATTAGATTAGGAGCACTTTGTTTTATTGTTGAGGCTGCTGGTGTTGGTAACATGATTTCGAGGGAAAGGGCGTGTCAATCGATTGGATTGGTTGGTCTTTCAAGACAAGGTAACGTGCTTGTGGAGTTGTTTAGTGATGGAGATAACACTACAAAACTTGTGGCCGGTAATACTCTTGGTGTGCTATTGAACCATGTTGATTACATCAGACCAATTGCTCAAGCTGGGGCTATACCTCTTTATGCGGAGCTTCTTCAAGGAGATGATGTTTCTGGTAAGGAGATAGCCGAGGATGTGTTTTGTATATTGGCTGTTGCAGAGGCGAATGCTGTTGAAATTGTGGGTCATTTGGTGAGACTTCTTAGAGAAGGTGATGACGAAGCTAAAGCAGCAGCTGTCGATGTGATATGGGATCTGTTGCGATACAAGCACACACCTTCTGTGATCCGGAACTCAGGTGCGGCTTTTATCCTTGTTGAGTTGTTGAGCCATGGGACTGAAGAAATAAAGAAAAAGATTTCTGGGGCTTTTTCTCAGATGAGCTACCAGGAAAGTGGTAGAATGGTCCTTGGTGATGCAAGAGCAATTCCGGTTCTTATTGATTTACTACATGACGAGACTGGGGAACTAAGAAATAATGTGGTTGAGGCTCTTTCTAATTTTCATGAGGATCCATTGTATCATGATATATTATCTGATGTAAATAGACTAATTCGTATTCATGCATCGAATGGGCATATGCCTAGATCTTTATGTAGAACGAGTGTTGAGCAACTCATTTGGATCCTGGATCATGTTTAA
Protein sequence

>Msa1225940

MGEHGVARSLLRLLPRAEGKMQKVLIECLLVVVSFCDNSRTFVATNGGLEIIISLLSSCSDDVRLYLLEILSALALQMEVRRELIRLGALCFIVEAAGVGNMISRERACQSIGLVGLSRQGNVLVELFSDGDNTTKLVAGNTLGVLLNHVDYIRPIAQAGAIPLYAELLQGDDVSGKEIAEDVFCILAVAEANAVEIVGHLVRLLREGDDEAKAAAVDVIWDLLRYKHTPSVIRNSGAAFILVELLSHGTEEIKKKISGAFSQMSYQESGRMVLGDARAIPVLIDLLHDETGELRNNVVEALSNFHEDPLYHDILSDVNRLIRIHASNGHMPRSLCRTSVEQLIWILDHV*