Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0503170 | XP_003621910.1 | 63.592 | 206 | 64 | 2 | 1 | 195 | 1 | 206 | 2.33e-84 | 259 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0503170 | G7KXW3 | 63.592 | 206 | 64 | 2 | 1 | 195 | 1 | 206 | 1.11e-84 | 259 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0100080 | Msa0503170 | 0.804954 | 1.685377e-49 | -8.615850e-47 |
| Msa0503170 | Msa0503180 | 0.985929 | 4.041459e-165 | -8.615850e-47 |
| Msa0503170 | Msa0503190 | 0.941510 | 3.666901e-101 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0503170 | MtrunA17_Chr7g0223411 | 63.592 | 206 | 64 | 2 | 1 | 195 | 1 | 206 | 2.14e-88 | 259 |
| Msa0503170 | MtrunA17_Chr7g0223461 | 41.553 | 219 | 103 | 6 | 1 | 195 | 1 | 218 | 1.56e-45 | 150 |
| Msa0503170 | MtrunA17_Chr7g0223421 | 43.689 | 206 | 100 | 7 | 1 | 194 | 4 | 205 | 1.03e-42 | 144 |
| Msa0503170 | MtrunA17_Chr7g0223501 | 37.931 | 232 | 105 | 6 | 1 | 196 | 1 | 229 | 2.93e-42 | 142 |
| Msa0503170 | MtrunA17_Chr1g0162651 | 27.232 | 224 | 113 | 6 | 17 | 196 | 43 | 260 | 3.53e-18 | 80.5 |
| Msa0503170 | MtrunA17_Chr4g0034271 | 28.182 | 220 | 111 | 6 | 17 | 195 | 25 | 238 | 6.39e-16 | 73.9 |
| Msa0503170 | MtrunA17_Chr3g0101771 | 51.923 | 52 | 25 | 0 | 1 | 52 | 1 | 52 | 8.37e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0503170 | AT2G16050.1 | 27.511 | 229 | 123 | 8 | 4 | 195 | 13 | 235 | 4.66e-18 | 80.1 |
Find 30 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGAAGTGTTTCAAGGATA+TGG | 0.264954 | 3_4:+46139315 | Msa0503170:CDS |
| CATATGACCAACCTTCAAAT+TGG | 0.292759 | 3_4:-46139261 | None:intergenic |
| CGTGCTATCATGAAATTTAC+TGG | 0.315601 | 3_4:+46139382 | Msa0503170:CDS |
| TTACAATGAACACACTTTGA+TGG | 0.327459 | 3_4:-46139217 | None:intergenic |
| AATAATAATAAGAGGTCAAA+AGG | 0.360252 | 3_4:+46139352 | Msa0503170:CDS |
| GGTGTAGAGCATAGTCTGTT+TGG | 0.375990 | 3_4:-46139120 | None:intergenic |
| ATCAAAGTGTGTTCATTGTA+AGG | 0.384337 | 3_4:+46139219 | Msa0503170:CDS |
| GAAATTTACTGGCAAATTAG+TGG | 0.406057 | 3_4:+46139393 | Msa0503170:CDS |
| TTGCAATTGCACATTCTGTA+TGG | 0.422174 | 3_4:-46138964 | None:intergenic |
| GCTTGTGGAAAAGATTTGTT+AGG | 0.436947 | 3_4:+46139079 | Msa0503170:CDS |
| GTGTAGAGCATAGTCTGTTT+GGG | 0.456600 | 3_4:-46139119 | None:intergenic |
| AAAGTTTGAATAATAATAAG+AGG | 0.467019 | 3_4:+46139344 | Msa0503170:CDS |
| TGTTCATGTGAAGTGTTTCA+AGG | 0.490839 | 3_4:+46139309 | Msa0503170:CDS |
| TCAACACTAGATCAAGAACC+TGG | 0.506748 | 3_4:+46139750 | Msa0503170:CDS |
| GTTGCGAGGAACCAATTTGA+AGG | 0.507705 | 3_4:+46139250 | Msa0503170:CDS |
| TTTAAGTTACGTGAGAAAGC+AGG | 0.518670 | 3_4:+46139037 | Msa0503170:intron |
| ATGGTAGCCATAAAACTAGT+AGG | 0.537312 | 3_4:-46139448 | None:intergenic |
| ATAATAATAAGAGGTCAAAA+GGG | 0.541908 | 3_4:+46139353 | Msa0503170:CDS |
| TACATTACAATTGAACGTTG+TGG | 0.546479 | 3_4:+46138853 | Msa0503170:CDS |
| CATAGTCTGTTTGGGAACAA+TGG | 0.556656 | 3_4:-46139111 | None:intergenic |
| TAATGTATGTTGATGATCCA+TGG | 0.558887 | 3_4:-46138837 | None:intergenic |
| ATGTAGTTAATTCTGATGAA+AGG | 0.564478 | 3_4:+46139281 | Msa0503170:intron |
| TTACGTGAGAAAGCAGGACC+AGG | 0.592128 | 3_4:+46139043 | Msa0503170:CDS |
| TCTTTCAGCTTCAAACAACA+AGG | 0.592460 | 3_4:-46139142 | None:intergenic |
| CTTTCAGCTTCAAACAACAA+GGG | 0.622276 | 3_4:-46139141 | None:intergenic |
| CAAGCATCACAGAATCCACC+TGG | 0.622946 | 3_4:-46139061 | None:intergenic |
| GGTGGATTCTGTGATGCTTG+TGG | 0.625129 | 3_4:+46139064 | Msa0503170:CDS |
| CAACACTAGATCAAGAACCT+GGG | 0.665440 | 3_4:+46139751 | Msa0503170:CDS |
| CGTGAGAAAGCAGGACCAGG+TGG | 0.698735 | 3_4:+46139046 | Msa0503170:CDS |
| GTAAGGATATGCATGTTGCG+AGG | 0.703293 | 3_4:+46139236 | Msa0503170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAAGCATTTATTGAAAAA+TGG | - | chr3_4:46139019-46139038 | None:intergenic | 15.0% |
| !! | AAAGTTTGAATAATAATAAG+AGG | + | chr3_4:46139344-46139363 | Msa0503170:CDS | 15.0% |
| !! | TATTTGAATACAATCTTAAA+AGG | - | chr3_4:46139606-46139625 | None:intergenic | 15.0% |
| !! | ATTGTATTCAAATATTGTAA+TGG | + | chr3_4:46139612-46139631 | Msa0503170:intron | 15.0% |
| !!! | AAAAAGCATTTATTGAAAAA+TGG | - | chr3_4:46139019-46139038 | None:intergenic | 15.0% |
| !! | AAAGTTTGAATAATAATAAG+AGG | + | chr3_4:46139344-46139363 | Msa0503170:CDS | 15.0% |
| !! | TATTTGAATACAATCTTAAA+AGG | - | chr3_4:46139606-46139625 | None:intergenic | 15.0% |
| !! | ATTGTATTCAAATATTGTAA+TGG | + | chr3_4:46139612-46139631 | Msa0503170:intron | 15.0% |
| !! | TTTATTGAAAAATGGATGAA+CGG | - | chr3_4:46139011-46139030 | None:intergenic | 20.0% |
| !!! | GATAAATTTTGTGATAAAGA+TGG | + | chr3_4:46139163-46139182 | Msa0503170:CDS | 20.0% |
| !! | AATAATAATAAGAGGTCAAA+AGG | + | chr3_4:46139352-46139371 | Msa0503170:CDS | 20.0% |
| !! | ATAATAATAAGAGGTCAAAA+GGG | + | chr3_4:46139353-46139372 | Msa0503170:CDS | 20.0% |
| !! | TAGTACTACCAATATAATTT+CGG | + | chr3_4:46139514-46139533 | Msa0503170:intron | 20.0% |
| !!! | TTAAGTTTTATAAATTTCCC+AGG | - | chr3_4:46139771-46139790 | None:intergenic | 20.0% |
| !! | TTTATTGAAAAATGGATGAA+CGG | - | chr3_4:46139011-46139030 | None:intergenic | 20.0% |
| !!! | GATAAATTTTGTGATAAAGA+TGG | + | chr3_4:46139163-46139182 | Msa0503170:CDS | 20.0% |
| !! | AATAATAATAAGAGGTCAAA+AGG | + | chr3_4:46139352-46139371 | Msa0503170:CDS | 20.0% |
| !! | ATAATAATAAGAGGTCAAAA+GGG | + | chr3_4:46139353-46139372 | Msa0503170:CDS | 20.0% |
| !! | TAGTACTACCAATATAATTT+CGG | + | chr3_4:46139514-46139533 | Msa0503170:intron | 20.0% |
| !!! | TTAAGTTTTATAAATTTCCC+AGG | - | chr3_4:46139771-46139790 | None:intergenic | 20.0% |
| ! | ATGTAGTTAATTCTGATGAA+AGG | + | chr3_4:46139281-46139300 | Msa0503170:intron | 25.0% |
| !! | AGTTGATACCGAAATTATAT+TGG | - | chr3_4:46139525-46139544 | None:intergenic | 25.0% |
| ! | GTACATGTGTATTTCTTTAA+TGG | - | chr3_4:46139674-46139693 | None:intergenic | 25.0% |
| ! | ATGTAGTTAATTCTGATGAA+AGG | + | chr3_4:46139281-46139300 | Msa0503170:intron | 25.0% |
| !! | AGTTGATACCGAAATTATAT+TGG | - | chr3_4:46139525-46139544 | None:intergenic | 25.0% |
| ! | GTACATGTGTATTTCTTTAA+TGG | - | chr3_4:46139674-46139693 | None:intergenic | 25.0% |
| TACATTACAATTGAACGTTG+TGG | + | chr3_4:46138853-46138872 | Msa0503170:CDS | 30.0% | |
| ! | TTTTGTAAAGAGCTAGGTTA+TGG | + | chr3_4:46138899-46138918 | Msa0503170:CDS | 30.0% |
| TTACAATGAACACACTTTGA+TGG | - | chr3_4:46139220-46139239 | None:intergenic | 30.0% | |
| ATCAAAGTGTGTTCATTGTA+AGG | + | chr3_4:46139219-46139238 | Msa0503170:CDS | 30.0% | |
| GAAATTTACTGGCAAATTAG+TGG | + | chr3_4:46139393-46139412 | Msa0503170:CDS | 30.0% | |
| !!! | ACTAATCGACGTTGTTTTAA+TGG | + | chr3_4:46139423-46139442 | Msa0503170:CDS | 30.0% |
| !!! | AATGGATCCTACTAGTTTTA+TGG | + | chr3_4:46139441-46139460 | Msa0503170:CDS | 30.0% |
| ACGAGCTATAAGTTAACTTA+TGG | + | chr3_4:46139550-46139569 | Msa0503170:intron | 30.0% | |
| TACATTACAATTGAACGTTG+TGG | + | chr3_4:46138853-46138872 | Msa0503170:CDS | 30.0% | |
| ! | TTTTGTAAAGAGCTAGGTTA+TGG | + | chr3_4:46138899-46138918 | Msa0503170:CDS | 30.0% |
| TTACAATGAACACACTTTGA+TGG | - | chr3_4:46139220-46139239 | None:intergenic | 30.0% | |
| ATCAAAGTGTGTTCATTGTA+AGG | + | chr3_4:46139219-46139238 | Msa0503170:CDS | 30.0% | |
| GAAATTTACTGGCAAATTAG+TGG | + | chr3_4:46139393-46139412 | Msa0503170:CDS | 30.0% | |
| !!! | ACTAATCGACGTTGTTTTAA+TGG | + | chr3_4:46139423-46139442 | Msa0503170:CDS | 30.0% |
| !!! | AATGGATCCTACTAGTTTTA+TGG | + | chr3_4:46139441-46139460 | Msa0503170:CDS | 30.0% |
| ACGAGCTATAAGTTAACTTA+TGG | + | chr3_4:46139550-46139569 | Msa0503170:intron | 30.0% | |
| ! | TGCAGTTTTTGTAAAGAGCT+AGG | + | chr3_4:46138893-46138912 | Msa0503170:CDS | 35.0% |
| TTGCAATTGCACATTCTGTA+TGG | - | chr3_4:46138967-46138986 | None:intergenic | 35.0% | |
| ! | TTTAAGTTACGTGAGAAAGC+AGG | + | chr3_4:46139037-46139056 | Msa0503170:intron | 35.0% |
| ! | GCTTGTGGAAAAGATTTGTT+AGG | + | chr3_4:46139079-46139098 | Msa0503170:CDS | 35.0% |
| CTTTCAGCTTCAAACAACAA+GGG | - | chr3_4:46139144-46139163 | None:intergenic | 35.0% | |
| TCTTTCAGCTTCAAACAACA+AGG | - | chr3_4:46139145-46139164 | None:intergenic | 35.0% | |
| CATATGACCAACCTTCAAAT+TGG | - | chr3_4:46139264-46139283 | None:intergenic | 35.0% | |
| ! | TGTTCATGTGAAGTGTTTCA+AGG | + | chr3_4:46139309-46139328 | Msa0503170:CDS | 35.0% |
| ! | TGTGAAGTGTTTCAAGGATA+TGG | + | chr3_4:46139315-46139334 | Msa0503170:CDS | 35.0% |
| CGTGCTATCATGAAATTTAC+TGG | + | chr3_4:46139382-46139401 | Msa0503170:CDS | 35.0% | |
| ATGGTAGCCATAAAACTAGT+AGG | - | chr3_4:46139451-46139470 | None:intergenic | 35.0% | |
| !!! | TAGTTTTATGGCTACCATTG+TGG | + | chr3_4:46139453-46139472 | Msa0503170:intron | 35.0% |
| ! | TGCAGTTTTTGTAAAGAGCT+AGG | + | chr3_4:46138893-46138912 | Msa0503170:CDS | 35.0% |
| TTGCAATTGCACATTCTGTA+TGG | - | chr3_4:46138967-46138986 | None:intergenic | 35.0% | |
| ! | TTTAAGTTACGTGAGAAAGC+AGG | + | chr3_4:46139037-46139056 | Msa0503170:intron | 35.0% |
| ! | GCTTGTGGAAAAGATTTGTT+AGG | + | chr3_4:46139079-46139098 | Msa0503170:CDS | 35.0% |
| CTTTCAGCTTCAAACAACAA+GGG | - | chr3_4:46139144-46139163 | None:intergenic | 35.0% | |
| TCTTTCAGCTTCAAACAACA+AGG | - | chr3_4:46139145-46139164 | None:intergenic | 35.0% | |
| CATATGACCAACCTTCAAAT+TGG | - | chr3_4:46139264-46139283 | None:intergenic | 35.0% | |
| ! | TGTTCATGTGAAGTGTTTCA+AGG | + | chr3_4:46139309-46139328 | Msa0503170:CDS | 35.0% |
| ! | TGTGAAGTGTTTCAAGGATA+TGG | + | chr3_4:46139315-46139334 | Msa0503170:CDS | 35.0% |
| CGTGCTATCATGAAATTTAC+TGG | + | chr3_4:46139382-46139401 | Msa0503170:CDS | 35.0% | |
| ATGGTAGCCATAAAACTAGT+AGG | - | chr3_4:46139451-46139470 | None:intergenic | 35.0% | |
| !!! | TAGTTTTATGGCTACCATTG+TGG | + | chr3_4:46139453-46139472 | Msa0503170:intron | 35.0% |
| !!! | TGCAATTGCTGTTTTCACAG+TGG | - | chr3_4:46138934-46138953 | None:intergenic | 40.0% |
| CATAGTCTGTTTGGGAACAA+TGG | - | chr3_4:46139114-46139133 | None:intergenic | 40.0% | |
| GTGTAGAGCATAGTCTGTTT+GGG | - | chr3_4:46139122-46139141 | None:intergenic | 40.0% | |
| TCAACACTAGATCAAGAACC+TGG | + | chr3_4:46139750-46139769 | Msa0503170:CDS | 40.0% | |
| CAACACTAGATCAAGAACCT+GGG | + | chr3_4:46139751-46139770 | Msa0503170:CDS | 40.0% | |
| !!! | TGCAATTGCTGTTTTCACAG+TGG | - | chr3_4:46138934-46138953 | None:intergenic | 40.0% |
| CATAGTCTGTTTGGGAACAA+TGG | - | chr3_4:46139114-46139133 | None:intergenic | 40.0% | |
| GTGTAGAGCATAGTCTGTTT+GGG | - | chr3_4:46139122-46139141 | None:intergenic | 40.0% | |
| TCAACACTAGATCAAGAACC+TGG | + | chr3_4:46139750-46139769 | Msa0503170:CDS | 40.0% | |
| CAACACTAGATCAAGAACCT+GGG | + | chr3_4:46139751-46139770 | Msa0503170:CDS | 40.0% | |
| GGTGTAGAGCATAGTCTGTT+TGG | - | chr3_4:46139123-46139142 | None:intergenic | 45.0% | |
| GTAAGGATATGCATGTTGCG+AGG | + | chr3_4:46139236-46139255 | Msa0503170:CDS | 45.0% | |
| GTTGCGAGGAACCAATTTGA+AGG | + | chr3_4:46139250-46139269 | Msa0503170:CDS | 45.0% | |
| CGAGGAACCAATTTGAAGGT+TGG | + | chr3_4:46139254-46139273 | Msa0503170:intron | 45.0% | |
| GGTGTAGAGCATAGTCTGTT+TGG | - | chr3_4:46139123-46139142 | None:intergenic | 45.0% | |
| GTAAGGATATGCATGTTGCG+AGG | + | chr3_4:46139236-46139255 | Msa0503170:CDS | 45.0% | |
| GTTGCGAGGAACCAATTTGA+AGG | + | chr3_4:46139250-46139269 | Msa0503170:CDS | 45.0% | |
| CGAGGAACCAATTTGAAGGT+TGG | + | chr3_4:46139254-46139273 | Msa0503170:intron | 45.0% | |
| !! | CTTATAAAATTATATTAAAA+AGG | - | chr3_4:46139651-46139670 | None:intergenic | 5.0% |
| !! | CTTATAAAATTATATTAAAA+AGG | - | chr3_4:46139651-46139670 | None:intergenic | 5.0% |
| TTACGTGAGAAAGCAGGACC+AGG | + | chr3_4:46139043-46139062 | Msa0503170:CDS | 50.0% | |
| CAAGCATCACAGAATCCACC+TGG | - | chr3_4:46139064-46139083 | None:intergenic | 50.0% | |
| ! | GGTGGATTCTGTGATGCTTG+TGG | + | chr3_4:46139064-46139083 | Msa0503170:CDS | 50.0% |
| CTCGAAAAAACCTGAAGCCG+CGG | - | chr3_4:46139482-46139501 | None:intergenic | 50.0% | |
| TTACGTGAGAAAGCAGGACC+AGG | + | chr3_4:46139043-46139062 | Msa0503170:CDS | 50.0% | |
| CAAGCATCACAGAATCCACC+TGG | - | chr3_4:46139064-46139083 | None:intergenic | 50.0% | |
| ! | GGTGGATTCTGTGATGCTTG+TGG | + | chr3_4:46139064-46139083 | Msa0503170:CDS | 50.0% |
| CTCGAAAAAACCTGAAGCCG+CGG | - | chr3_4:46139482-46139501 | None:intergenic | 50.0% | |
| CGTGAGAAAGCAGGACCAGG+TGG | + | chr3_4:46139046-46139065 | Msa0503170:CDS | 60.0% | |
| GGCTACCATTGTGGAAGCCG+CGG | + | chr3_4:46139462-46139481 | Msa0503170:intron | 60.0% | |
| TGAAGCCGCGGCTTCCACAA+TGG | - | chr3_4:46139470-46139489 | None:intergenic | 60.0% | |
| ATTGTGGAAGCCGCGGCTTC+AGG | + | chr3_4:46139469-46139488 | Msa0503170:intron | 60.0% | |
| CGTGAGAAAGCAGGACCAGG+TGG | + | chr3_4:46139046-46139065 | Msa0503170:CDS | 60.0% | |
| GGCTACCATTGTGGAAGCCG+CGG | + | chr3_4:46139462-46139481 | Msa0503170:intron | 60.0% | |
| TGAAGCCGCGGCTTCCACAA+TGG | - | chr3_4:46139470-46139489 | None:intergenic | 60.0% | |
| ATTGTGGAAGCCGCGGCTTC+AGG | + | chr3_4:46139469-46139488 | Msa0503170:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 46138839 | 46139794 | 46138839 | ID=Msa0503170;Name=Msa0503170 |
| chr3_4 | mRNA | 46138839 | 46139794 | 46138839 | ID=Msa0503170-mRNA-1;Parent=Msa0503170;Name=Msa0503170-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|0.75|0|0|4|0|220 |
| chr3_4 | exon | 46138839 | 46139009 | 46138839 | ID=Msa0503170-mRNA-1:exon:9869;Parent=Msa0503170-mRNA-1 |
| chr3_4 | exon | 46139043 | 46139271 | 46139043 | ID=Msa0503170-mRNA-1:exon:9870;Parent=Msa0503170-mRNA-1 |
| chr3_4 | exon | 46139287 | 46139471 | 46139287 | ID=Msa0503170-mRNA-1:exon:9871;Parent=Msa0503170-mRNA-1 |
| chr3_4 | exon | 46139717 | 46139794 | 46139717 | ID=Msa0503170-mRNA-1:exon:9872;Parent=Msa0503170-mRNA-1 |
| chr3_4 | CDS | 46138839 | 46139009 | 46138839 | ID=Msa0503170-mRNA-1:cds;Parent=Msa0503170-mRNA-1 |
| chr3_4 | CDS | 46139043 | 46139271 | 46139043 | ID=Msa0503170-mRNA-1:cds;Parent=Msa0503170-mRNA-1 |
| chr3_4 | CDS | 46139287 | 46139471 | 46139287 | ID=Msa0503170-mRNA-1:cds;Parent=Msa0503170-mRNA-1 |
| chr3_4 | CDS | 46139717 | 46139794 | 46139717 | ID=Msa0503170-mRNA-1:cds;Parent=Msa0503170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
