AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0503840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0503840 MtrunA17_Chr8g0392721 71.795 39 11 0 66 104 12 50 1.36e-15 67.0
Msa0503840 MtrunA17_Chr1g0199791 66.000 50 16 1 12 61 1 49 4.30e-12 56.6
Msa0503840 MtrunA17_Chr3g0120701 72.727 33 9 0 66 98 12 44 8.64e-12 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCGCAGCCTACTGCTGTT+GGG 0.324874 3_4:-47484680 Msa0503840:CDS
TGAACTCGGCGGCAGATCTA+TGG 0.342117 3_4:-47487351 Msa0503840:CDS
TTATTCTTCTCACCTCTTGC+CGG 0.378620 3_4:-47487281 Msa0503840:CDS
AACTTCTGTTTGTTCCTGTA+AGG 0.384138 3_4:-47484730 Msa0503840:CDS
TTGTCCACAACAGACCTTAC+AGG 0.423953 3_4:+47484716 None:intergenic
TTTCCGCAGCCTACTGCTGT+TGG 0.429558 3_4:-47484681 Msa0503840:CDS
TTGTGCGGCAGTTGCGGAGT+CGG 0.437749 3_4:+47487222 None:intergenic
ATAACCCTATGTGTTGTGTT+AGG 0.454244 3_4:+47487300 None:intergenic
TCCGCAGCCTACTGCTGTTG+GGG 0.456756 3_4:-47484679 Msa0503840:CDS
TCTTGGTGTGCAGTGTAGGC+TGG 0.481877 3_4:-47484767 Msa0503840:intron
ATCATCTCCTCACTGGAAAC+CGG 0.502488 3_4:-47487377 None:intergenic
TTGTTGTTGTGCGGCAGTTG+CGG 0.505008 3_4:+47487216 None:intergenic
CCGCAGCCTACTGCTGTTGG+GGG 0.543395 3_4:-47484678 Msa0503840:CDS
TGTTCCTGTAAGGTCTGTTG+TGG 0.543606 3_4:-47484720 Msa0503840:CDS
CTGGAAACCGGCGCTGAACT+CGG 0.546923 3_4:-47487365 Msa0503840:CDS
TCTGCCGCCGAGTTCAGCGC+CGG 0.573111 3_4:+47487358 None:intergenic
GGTGTGCAGTGTAGGCTGGA+GGG 0.577637 3_4:-47484763 Msa0503840:intron
GGCTGCGGAAACACAGACCT+GGG 0.579722 3_4:+47484693 None:intergenic
TCGGCGAGATGTGCGTGGAT+CGG 0.580720 3_4:+47487241 None:intergenic
ATGACCTTCGTAACTATCAC+CGG 0.588059 3_4:-47487193 Msa0503840:intron
TGGTGTGCAGTGTAGGCTGG+AGG 0.595874 3_4:-47484764 Msa0503840:intron
AGGCTGCGGAAACACAGACC+TGG 0.597241 3_4:+47484692 None:intergenic
GAAGGTCATTTGTTGTTGTG+CGG 0.601943 3_4:+47487207 None:intergenic
CGGAGTCGGCGAGATGTGCG+TGG 0.602009 3_4:+47487236 None:intergenic
AGGACCTAACACAACACATA+GGG 0.603430 3_4:-47487304 Msa0503840:CDS
GAGGACCTAACACAACACAT+AGG 0.616752 3_4:-47487305 Msa0503840:CDS
AGGTCTGTTGTGGACAACCC+AGG 0.617858 3_4:-47484710 Msa0503840:CDS
GAGTCTCGACGTTCATAACG+AGG 0.648715 3_4:-47487324 Msa0503840:CDS
GTTACCGGTGATAGTTACGA+AGG 0.714605 3_4:+47487189 None:intergenic
GAAACCGGCGCTGAACTCGG+CGG 0.757139 3_4:-47487362 Msa0503840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATATATAAAAAGAAAT+TGG + chr3_4:47487220-47487239 None:intergenic 10.0%
!!! AATTTCTTTTTATATATTCT+TGG - chr3_4:47487219-47487238 Msa0503840:CDS 10.0%
!! CTAAATAATTTCAACAAAAA+TGG + chr3_4:47485141-47485160 None:intergenic 15.0%
!! AATATTAAACAATTCTGAAT+TGG + chr3_4:47486055-47486074 None:intergenic 15.0%
!! TGTATTTCCTTAAATTTAAT+AGG - chr3_4:47486122-47486141 Msa0503840:intron 15.0%
!! TAATTTCGAATTTGACATTA+GGG - chr3_4:47484885-47484904 Msa0503840:intron 20.0%
!!! ATTTATGTTTTTCGAATAGT+AGG - chr3_4:47485167-47485186 Msa0503840:intron 20.0%
!!! CAAACTAACAAAACTTTTTT+TGG + chr3_4:47485504-47485523 None:intergenic 20.0%
!!! CAAAAAAAGTTTTGTTAGTT+TGG - chr3_4:47485502-47485521 Msa0503840:intron 20.0%
!!! TCTTAAAGCTTTTAAATAGA+TGG - chr3_4:47485545-47485564 Msa0503840:intron 20.0%
!! TGTGAAATTATCAAATTTGA+AGG - chr3_4:47485772-47485791 Msa0503840:intron 20.0%
!!! GTCATTTGAGTTAAAATATT+TGG - chr3_4:47485932-47485951 Msa0503840:intron 20.0%
!! TCCTTAAATTTAATAGGATT+TGG - chr3_4:47486128-47486147 Msa0503840:intron 20.0%
!!! GATTTTCAATATTTGCTTTA+TGG - chr3_4:47486506-47486525 Msa0503840:intron 20.0%
!!! TTTTTTTCTTGAATTGACTT+TGG - chr3_4:47486629-47486648 Msa0503840:intron 20.0%
!! ACAAATAAATGTATGTTATG+TGG + chr3_4:47486680-47486699 None:intergenic 20.0%
!! ACATAACATACATTTATTTG+TGG - chr3_4:47486679-47486698 Msa0503840:intron 20.0%
!! CATAACATACATTTATTTGT+GGG - chr3_4:47486680-47486699 Msa0503840:intron 20.0%
!! ATAACATACATTTATTTGTG+GGG - chr3_4:47486681-47486700 Msa0503840:intron 20.0%
!!! TGTAGTTACAATAAGATTTT+GGG - chr3_4:47487165-47487184 Msa0503840:intron 20.0%
! CTAATTTCGAATTTGACATT+AGG - chr3_4:47484884-47484903 Msa0503840:intron 25.0%
! CTCAAACAAGATGATAAAAA+AGG - chr3_4:47485195-47485214 Msa0503840:intron 25.0%
! AGAGTGAAATAAAATGGAAA+AGG + chr3_4:47485267-47485286 None:intergenic 25.0%
!!! CTATTTAAAAGCTTTAAGAC+AGG + chr3_4:47485544-47485563 None:intergenic 25.0%
! TTTCACAATGTCAAAATCAA+AGG + chr3_4:47485759-47485778 None:intergenic 25.0%
!!! AGTTTTGAGAAAATGACTTA+CGG + chr3_4:47485831-47485850 None:intergenic 25.0%
! TAAATGCACTAAGTTACTAT+TGG - chr3_4:47485982-47486001 Msa0503840:intron 25.0%
! GCCAAATCCTATTAAATTTA+AGG + chr3_4:47486132-47486151 None:intergenic 25.0%
! AATTTATAACCACTTTGATC+AGG + chr3_4:47486344-47486363 None:intergenic 25.0%
! CCTAAAAATCAAAGTTTGAT+CGG + chr3_4:47486375-47486394 None:intergenic 25.0%
!!! TTTTTCAATGCAAATCATGA+TGG + chr3_4:47486457-47486476 None:intergenic 25.0%
! TCAATAACATGTAGAAATGA+TGG + chr3_4:47486560-47486579 None:intergenic 25.0%
! TGCTATCATATAATTTGATG+AGG + chr3_4:47486606-47486625 None:intergenic 25.0%
! ATAAATGTATGTTATGTGGA+TGG + chr3_4:47486676-47486695 None:intergenic 25.0%
! ATCAATTGATAAGAAATTGC+AGG + chr3_4:47487060-47487079 None:intergenic 25.0%
! TGCAATTTCTTATCAATTGA+TGG - chr3_4:47487059-47487078 Msa0503840:intron 25.0%
! ATATTTCGTGTATGAAATGT+TGG - chr3_4:47487099-47487118 Msa0503840:intron 25.0%
!! GTGTAGTTACAATAAGATTT+TGG - chr3_4:47487164-47487183 Msa0503840:intron 25.0%
!! GTAGTTACAATAAGATTTTG+GGG - chr3_4:47487166-47487185 Msa0503840:intron 25.0%
!! TAGTTACAATAAGATTTTGG+GGG - chr3_4:47487167-47487186 Msa0503840:intron 25.0%
CAGTCAATATGAACATGAAT+CGG + chr3_4:47484853-47484872 None:intergenic 30.0%
TAGCAGTTGCAATTTAACAT+AGG - chr3_4:47485046-47485065 Msa0503840:intron 30.0%
CTTGGTAGAGTGAAATAAAA+TGG + chr3_4:47485273-47485292 None:intergenic 30.0%
!! AGTTGTGATCTTTGAGTTTT+AGG - chr3_4:47485329-47485348 Msa0503840:intron 30.0%
!! GATCAGAAAGTATTGTAGTT+TGG - chr3_4:47485367-47485386 Msa0503840:intron 30.0%
TGACAATAACTATTTCCCTT+CGG - chr3_4:47485589-47485608 Msa0503840:intron 30.0%
GAATCATCAGAAAAATAAGG+CGG + chr3_4:47485644-47485663 None:intergenic 30.0%
!!! GCCTTATTTTTCTGATGATT+CGG - chr3_4:47485643-47485662 Msa0503840:intron 30.0%
ACCGAATCATCAGAAAAATA+AGG + chr3_4:47485647-47485666 None:intergenic 30.0%
ACATCTAAGAATTACCTCAA+TGG + chr3_4:47485675-47485694 None:intergenic 30.0%
GAGAATGATATGATACCATA+CGG + chr3_4:47485703-47485722 None:intergenic 30.0%
CAAACTGAAGGAAGTAAATT+AGG + chr3_4:47486225-47486244 None:intergenic 30.0%
CTAATTTACTTCCTTCAGTT+TGG - chr3_4:47486223-47486242 Msa0503840:intron 30.0%
TTGGGTTAATATACAACCTA+TGG - chr3_4:47486249-47486268 Msa0503840:intron 30.0%
AAATTGAATCCTGATCAAAG+TGG - chr3_4:47486332-47486351 Msa0503840:intron 30.0%
!! CCGATCAAACTTTGATTTTT+AGG - chr3_4:47486372-47486391 Msa0503840:intron 30.0%
CAACATAAGCAAACAATACA+TGG + chr3_4:47486487-47486506 None:intergenic 30.0%
! TCTAAGACAGAACAGAATAA+GGG + chr3_4:47487000-47487019 None:intergenic 30.0%
! ATCTAAGACAGAACAGAATA+AGG + chr3_4:47487001-47487020 None:intergenic 30.0%
ATAACCCTATGTGTTGTGTT+AGG + chr3_4:47484706-47484725 None:intergenic 35.0%
! GGTGAGAGAAATAGATTTTC+CGG + chr3_4:47484744-47484763 None:intergenic 35.0%
CAATATGAACATGAATCGGT+GGG + chr3_4:47484849-47484868 None:intergenic 35.0%
TCAATATGAACATGAATCGG+TGG + chr3_4:47484850-47484869 None:intergenic 35.0%
CAAAACAAAAGCTTGAAGCT+TGG + chr3_4:47485291-47485310 None:intergenic 35.0%
!!! AGCTTCAAGCTTTTGTTTTG+TGG - chr3_4:47485291-47485310 Msa0503840:intron 35.0%
GAGAGAATAGTAAATTCACC+TGG + chr3_4:47485482-47485501 None:intergenic 35.0%
TCTAAGAATTACCTCAATGG+TGG + chr3_4:47485672-47485691 None:intergenic 35.0%
GCTACAATGTCAAATAGCTA+TGG - chr3_4:47485794-47485813 Msa0503840:intron 35.0%
CTCTCAATCTCTTATTGTAG+TGG - chr3_4:47485849-47485868 Msa0503840:intron 35.0%
TGCTTATGATCACACAAATG+TGG + chr3_4:47486009-47486028 None:intergenic 35.0%
ATATTCTTCACTCTCTCCAT+AGG + chr3_4:47486268-47486287 None:intergenic 35.0%
GAATGCTTGCAAGAACAAAA+TGG + chr3_4:47486421-47486440 None:intergenic 35.0%
! CATTTATTTGTGGGGTTTTG+TGG - chr3_4:47486689-47486708 Msa0503840:intron 35.0%
CTGTGAAAAATTCTAGCAGA+TGG - chr3_4:47486882-47486901 Msa0503840:intron 35.0%
AAAGACAGACACAAAACACT+TGG + chr3_4:47486954-47486973 None:intergenic 35.0%
CTAAGACAGAACAGAATAAG+GGG + chr3_4:47486999-47487018 None:intergenic 35.0%
ATGAAATGTTGGACATGCTT+CGG - chr3_4:47487110-47487129 Msa0503840:intron 35.0%
CTAGTTCATATTCATACCCT+TGG + chr3_4:47487136-47487155 None:intergenic 35.0%
GGTATGAATATGAACTAGCA+TGG - chr3_4:47487138-47487157 Msa0503840:intron 35.0%
AACTTCTGTTTGTTCCTGTA+AGG - chr3_4:47487273-47487292 Msa0503840:CDS 35.0%
AGGACCTAACACAACACATA+GGG - chr3_4:47484699-47484718 Msa0503840:CDS 40.0%
TTATTCTTCTCACCTCTTGC+CGG - chr3_4:47484722-47484741 Msa0503840:CDS 40.0%
! GAAATAGATTTTCCGGCAAG+AGG + chr3_4:47484737-47484756 None:intergenic 40.0%
!! GAAGGTCATTTGTTGTTGTG+CGG + chr3_4:47484799-47484818 None:intergenic 40.0%
ATGACCTTCGTAACTATCAC+CGG - chr3_4:47484810-47484829 Msa0503840:intron 40.0%
TAGTTTGGATTGAGTGCAGA+GGG - chr3_4:47485382-47485401 Msa0503840:intron 40.0%
AATAAGGCGGCTTCACAATT+AGG + chr3_4:47485631-47485650 None:intergenic 40.0%
TCTTAGATGTGCTTACCGTA+TGG - chr3_4:47485685-47485704 Msa0503840:intron 40.0%
GTAGTGGTTGAATAGCTTCA+AGG - chr3_4:47485865-47485884 Msa0503840:intron 40.0%
ACTTCCTTCAGTTTGGTTGT+TGG - chr3_4:47486230-47486249 Msa0503840:intron 40.0%
CTTCCTTCAGTTTGGTTGTT+GGG - chr3_4:47486231-47486250 Msa0503840:intron 40.0%
TAACCCAACAACCAAACTGA+AGG + chr3_4:47486237-47486256 None:intergenic 40.0%
! ATATTCTTGGTGTGCAGTGT+AGG - chr3_4:47487232-47487251 Msa0503840:CDS 40.0%
GAGGACCTAACACAACACAT+AGG - chr3_4:47484698-47484717 Msa0503840:CDS 45.0%
GTTACCGGTGATAGTTACGA+AGG + chr3_4:47484817-47484836 None:intergenic 45.0%
ATCACAACTGTCACTCTGTC+AGG + chr3_4:47485318-47485337 None:intergenic 45.0%
GTAGTTTGGATTGAGTGCAG+AGG - chr3_4:47485381-47485400 Msa0503840:intron 45.0%
! CAGAGGGATGCTTTTGATGT+TGG - chr3_4:47485398-47485417 Msa0503840:intron 45.0%
! GGGATGCTTTTGATGTTGGA+CGG - chr3_4:47485402-47485421 Msa0503840:intron 45.0%
! CTGGCTCTCATATTGTTGTC+AGG + chr3_4:47485463-47485482 None:intergenic 45.0%
CTGACAACAATATGAGAGCC+AGG - chr3_4:47485461-47485480 Msa0503840:intron 45.0%
TTTCCAATGCACTCTCCGAA+GGG + chr3_4:47485607-47485626 None:intergenic 45.0%
! TTTTCCAATGCACTCTCCGA+AGG + chr3_4:47485608-47485627 None:intergenic 45.0%
!! TGATTCGGTTTCCACCATTG+AGG - chr3_4:47485658-47485677 Msa0503840:intron 45.0%
GCAGTGAACTGCATAACAAC+AGG + chr3_4:47486740-47486759 None:intergenic 45.0%
CACTGCGTGCATTTCTGTTA+TGG - chr3_4:47486754-47486773 Msa0503840:intron 45.0%
! ACCAGTTTTGCTGCTATGTG+TGG - chr3_4:47486788-47486807 Msa0503840:intron 45.0%
ACCACACATAGCAGCAAAAC+TGG + chr3_4:47486792-47486811 None:intergenic 45.0%
! TTTTGCTGCTATGTGTGGTC+TGG - chr3_4:47486793-47486812 Msa0503840:intron 45.0%
TTTGCTGCTATGTGTGGTCT+GGG - chr3_4:47486794-47486813 Msa0503840:intron 45.0%
TGTTCCTGTAAGGTCTGTTG+TGG - chr3_4:47487283-47487302 Msa0503840:CDS 45.0%
TTGTCCACAACAGACCTTAC+AGG + chr3_4:47487290-47487309 None:intergenic 45.0%
GAGTCTCGACGTTCATAACG+AGG - chr3_4:47484679-47484698 Msa0503840:CDS 50.0%
! TTGTTGTTGTGCGGCAGTTG+CGG + chr3_4:47484790-47484809 None:intergenic 50.0%
GGTGGGAGAAAGTGAGTTAC+CGG + chr3_4:47484832-47484851 None:intergenic 50.0%
TGATGTTGGACGGTGTTCAG+CGG - chr3_4:47485412-47485431 Msa0503840:intron 50.0%
! TTTCCCTTCGGAGAGTGCAT+TGG - chr3_4:47485601-47485620 Msa0503840:intron 50.0%
!! GGGTTTTGTGGCGTTTGTCT+CGG - chr3_4:47486701-47486720 Msa0503840:intron 50.0%
TGAACTCGGCGGCAGATCTA+TGG - chr3_4:47484652-47484671 Msa0503840:CDS 55.0%
TGGCGTTTGTCTCGGCTGTT+TGG - chr3_4:47486709-47486728 Msa0503840:intron 55.0%
GGCGTTTGTCTCGGCTGTTT+GGG - chr3_4:47486710-47486729 Msa0503840:intron 55.0%
CTATGTGTGGTCTGGGCATG+TGG - chr3_4:47486801-47486820 Msa0503840:intron 55.0%
TGTTGGACATGCTTCGGCCA+AGG - chr3_4:47487116-47487135 Msa0503840:intron 55.0%
GTTGGACATGCTTCGGCCAA+GGG - chr3_4:47487117-47487136 Msa0503840:intron 55.0%
! TCTTGGTGTGCAGTGTAGGC+TGG - chr3_4:47487236-47487255 Msa0503840:CDS 55.0%
AGGTCTGTTGTGGACAACCC+AGG - chr3_4:47487293-47487312 Msa0503840:CDS 55.0%
TTTCCGCAGCCTACTGCTGT+TGG - chr3_4:47487322-47487341 Msa0503840:CDS 55.0%
TTCCGCAGCCTACTGCTGTT+GGG - chr3_4:47487323-47487342 Msa0503840:CDS 55.0%
GGGGGAGTAGGTGATGTTCA+TGG - chr3_4:47487343-47487362 Msa0503840:CDS 55.0%
! CTGGAAACCGGCGCTGAACT+CGG - chr3_4:47484638-47484657 Msa0503840:CDS 60.0%
TCGGCGAGATGTGCGTGGAT+CGG + chr3_4:47484765-47484784 None:intergenic 60.0%
TTGTGCGGCAGTTGCGGAGT+CGG + chr3_4:47484784-47484803 None:intergenic 60.0%
TGGTGTGCAGTGTAGGCTGG+AGG - chr3_4:47487239-47487258 Msa0503840:CDS 60.0%
GGTGTGCAGTGTAGGCTGGA+GGG - chr3_4:47487240-47487259 Msa0503840:CDS 60.0%
GGCTGCGGAAACACAGACCT+GGG + chr3_4:47487313-47487332 None:intergenic 60.0%
AGGCTGCGGAAACACAGACC+TGG + chr3_4:47487314-47487333 None:intergenic 60.0%
TCCGCAGCCTACTGCTGTTG+GGG - chr3_4:47487324-47487343 Msa0503840:CDS 60.0%
CCTACTCCCCCAACAGCAGT+AGG + chr3_4:47487334-47487353 None:intergenic 60.0%
CCTACTGCTGTTGGGGGAGT+AGG - chr3_4:47487331-47487350 Msa0503840:CDS 60.0%
! GAAACCGGCGCTGAACTCGG+CGG - chr3_4:47484641-47484660 Msa0503840:CDS 65.0%
CCCCCAACAGCAGTAGGCTG+CGG + chr3_4:47487328-47487347 None:intergenic 65.0%
CCGCAGCCTACTGCTGTTGG+GGG - chr3_4:47487325-47487344 Msa0503840:CDS 65.0%
TCTGCCGCCGAGTTCAGCGC+CGG + chr3_4:47484648-47484667 None:intergenic 70.0%
CGGAGTCGGCGAGATGTGCG+TGG + chr3_4:47484770-47484789 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 47484638 47487387 47484638 ID=Msa0503840;Name=Msa0503840
chr3_4 mRNA 47484638 47487387 47484638 ID=Msa0503840-mRNA-1;Parent=Msa0503840;Name=Msa0503840-mRNA-1;_AED=0.52;_eAED=0.52;_QI=0|0|0|0.5|1|1|2|0|110
chr3_4 exon 47487194 47487387 47487194 ID=Msa0503840-mRNA-1:exon:10238;Parent=Msa0503840-mRNA-1
chr3_4 exon 47484638 47484776 47484638 ID=Msa0503840-mRNA-1:exon:10237;Parent=Msa0503840-mRNA-1
chr3_4 CDS 47487194 47487387 47487194 ID=Msa0503840-mRNA-1:cds;Parent=Msa0503840-mRNA-1
chr3_4 CDS 47484638 47484776 47484638 ID=Msa0503840-mRNA-1:cds;Parent=Msa0503840-mRNA-1
Gene Sequence

>Msa0503840

CTGGAAACCGGCGCTGAACTCGGCGGCAGATCTATGGCTTTGAGTCTCGACGTTCATAACGAGGACCTAACACAACACATAGGGTTATTCTTCTCACCTCTTGCCGGAAAATCTATTTCTCTCACCGATCCACGCACATCTCGCCGACTCCGCAACTGCCGCACAACAACAAATGACCTTCGTAACTATCACCGTGTAGGCTGGAGGGTGCTGTTAACAACTTCTGTTTGTTCCTGTAAGGTCTGTTGTGGACAACCCAGGTCTGTGTTTCCGCAGCCTACTGCTGTTGGGGGAGTAGGTGATGTTCATGGTTGTGTTCTTTTAGTTTTGTAA

Protein sequence

>Msa0503840

LETGAELGGRSMALSLDVHNEDLTQHIGLFFSPLAGKSISLTDPRTSRRLRNCRTTTNDLRNYHRVGWRVLLTTSVCSCKVCCGQPRSVFPQPTAVGGVGDVHGCVLLVL*