AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0719080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0719080 MtrunA17_Chr3g0125141 46.875 96 31 3 38 132 1 77 8.26e-16 69.3
Msa0719080 MtrunA17_Chr1g0197101 71.111 45 9 1 5 49 55 95 5.17e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTATTTCATTCGGAAAAT+TGG 0.104151 5_1:-13831600 Msa0719080:three_prime_UTR
CTGCAACTCAATTGAAATTA+TGG 0.134418 5_1:-13832067 Msa0719080:CDS
AAGACTCTGGGATGTCTTTA+TGG 0.210146 5_1:-13832026 Msa0719080:CDS
CATAATTTCAATTGAGTTGC+AGG 0.269649 5_1:+13832068 None:intergenic
GCTAATAAGCACACACGATT+TGG 0.289921 5_1:-13831827 Msa0719080:three_prime_UTR
GAAGGATGCTGTGATTATAT+TGG 0.291073 5_1:-13834171 Msa0719080:CDS
AGATTAGGACACATTCTATT+AGG 0.291663 5_1:-13834223 Msa0719080:CDS
TTTATTTCATTCGGAAAATT+GGG 0.295943 5_1:-13831599 Msa0719080:three_prime_UTR
GATGCAGGGCAGCTGGATAT+AGG 0.321032 5_1:-13832191 Msa0719080:CDS
TCTTTATGGTACTTGTGATA+TGG 0.350156 5_1:-13832012 Msa0719080:CDS
TGCAACTCAATTGAAATTAT+GGG 0.353224 5_1:-13832066 Msa0719080:CDS
GATGCAGGGGAGCGAGATGC+AGG 0.370231 5_1:-13832206 Msa0719080:CDS
ACGATGTCGGCCACCCTTGA+TGG 0.373602 5_1:+13831768 None:intergenic
TCTTGAGTAGGAAACATATA+AGG 0.375208 5_1:-13832139 Msa0719080:CDS
GCAGAAACTTGGCTACTATA+TGG 0.376503 5_1:+13831937 None:intergenic
GGAGAGACAACTGAAGGAAT+TGG 0.377514 5_1:-13831806 Msa0719080:three_prime_UTR
ATATTAATGTTTATTTCATT+CGG 0.380924 5_1:-13831608 Msa0719080:three_prime_UTR
AATGAAGGGGAGCGGGATGC+AGG 0.385018 5_1:-13832221 Msa0719080:CDS
AAATTATGGGCTGAATCGGC+TGG 0.391995 5_1:-13832053 Msa0719080:CDS
ATGCAGGGCAGCTGGATATA+GGG 0.408046 5_1:-13832190 Msa0719080:CDS
GGAAACATATAAGGAGAAGA+TGG 0.415058 5_1:-13832130 Msa0719080:CDS
GGAGCGAGATGCAGGGCAGC+TGG 0.422901 5_1:-13832198 Msa0719080:CDS
AGAAGGAAGGCATCTGATAA+TGG 0.449114 5_1:-13832107 Msa0719080:CDS
ATGGAGATACATGCGACTGC+TGG 0.450706 5_1:+13831956 None:intergenic
GCTGGGTAGGGATGATGCAC+CGG 0.458617 5_1:+13831974 None:intergenic
CTGCCGGATTGCTGCACAGA+TGG 0.465200 5_1:-13831918 Msa0719080:CDS
ATTGAAATTATGGGCTGAAT+CGG 0.472148 5_1:-13832057 Msa0719080:CDS
TCTGTGGACCTTTGACCTTA+CGG 0.478369 5_1:+13832369 None:intergenic
ATGGCAAAGCGCTACAAGCC+CGG 0.485664 5_1:-13831993 Msa0719080:CDS
GCAGCAATCCGGCAGAAACT+TGG 0.488047 5_1:+13831926 None:intergenic
TCAGAGACGTCATCATAGTC+TGG 0.489835 5_1:+13831739 None:intergenic
GATGTAAATCAGATGAATGA+AGG 0.491907 5_1:-13832236 Msa0719080:CDS
TCACTTTGATCAATATCGTC+AGG 0.494553 5_1:-13834147 Msa0719080:intron
CTGCCATCTGTGCAGCAATC+CGG 0.494932 5_1:+13831915 None:intergenic
TGAAGGGGAGCGGGATGCAG+GGG 0.496456 5_1:-13832219 Msa0719080:CDS
AATTATGGGCTGAATCGGCT+GGG 0.505977 5_1:-13832052 Msa0719080:CDS
TGAGAGTGTTATTTCCATCA+AGG 0.515577 5_1:-13831782 Msa0719080:three_prime_UTR
CTGGGTAGGGATGATGCACC+GGG 0.521803 5_1:+13831975 None:intergenic
GAATATTGATGAGACTTTGA+AGG 0.524229 5_1:-13834189 Msa0719080:CDS
AATAACTTTCATAACTGCAA+TGG 0.524765 5_1:+13831542 None:intergenic
TCAGATGAATGAAGGGGAGC+GGG 0.530853 5_1:-13832228 Msa0719080:CDS
ATGAAGGGGAGCGGGATGCA+GGG 0.531337 5_1:-13832220 Msa0719080:CDS
ATTATGGGCTGAATCGGCTG+GGG 0.531432 5_1:-13832051 Msa0719080:CDS
TGGAGATACATGCGACTGCT+GGG 0.532764 5_1:+13831957 None:intergenic
AAGGAGAAGATGGAGAAGGA+AGG 0.533403 5_1:-13832120 Msa0719080:CDS
GTCGCTTGTTTCAACTTATG+CGG 0.544680 5_1:+13832326 None:intergenic
GATACATGCGACTGCTGGGT+AGG 0.544893 5_1:+13831961 None:intergenic
ACGCTACAACACCATGGCAG+TGG 0.545516 5_1:-13832259 Msa0719080:CDS
CGCTACAACACCATGGCAGT+GGG 0.546579 5_1:-13832258 Msa0719080:CDS
AGTTGCAGGATCAACCTCCT+TGG 0.556412 5_1:+13832082 None:intergenic
ATGTAAATCAGATGAATGAA+GGG 0.559122 5_1:-13832235 Msa0719080:CDS
TATAGTAGCCAAGTTTCTGC+CGG 0.562202 5_1:-13831934 Msa0719080:CDS
ATCAGATGAATGAAGGGGAG+CGG 0.562953 5_1:-13832229 Msa0719080:CDS
ATACATGCGACTGCTGGGTA+GGG 0.563394 5_1:+13831962 None:intergenic
AAAGAAAACCGTAAGGTCAA+AGG 0.566284 5_1:-13832377 Msa0719080:CDS
ATGCAGGGGAGCGAGATGCA+GGG 0.577717 5_1:-13832205 Msa0719080:CDS
ATATAAGGAGAAGATGGAGA+AGG 0.579875 5_1:-13832124 Msa0719080:CDS
TTATTTCATTCGGAAAATTG+GGG 0.586712 5_1:-13831598 Msa0719080:three_prime_UTR
AGACGTCATCATAGTCTGGA+TGG 0.592265 5_1:+13831743 None:intergenic
CTCTACGCTGTCAACAAAGT+CGG 0.595260 5_1:+13831708 None:intergenic
GAGAGTGTTATTTCCATCAA+GGG 0.600370 5_1:-13831781 Msa0719080:three_prime_UTR
AGAGAGAGCAAATGCAGCAG+AGG 0.606952 5_1:-13831885 Msa0719080:three_prime_UTR
AGTCTGGATGGCGACGATGT+CGG 0.608407 5_1:+13831755 None:intergenic
GCTGTCAACAAAGTCGGCAT+CGG 0.608604 5_1:+13831714 None:intergenic
TGATGCTCGAGTTGCACGTG+AGG 0.614438 5_1:-13831861 Msa0719080:three_prime_UTR
GGCTCCTAAAGAAAACCGTA+AGG 0.618595 5_1:-13832384 Msa0719080:CDS
TGATTTACATCCCACTGCCA+TGG 0.619006 5_1:+13832248 None:intergenic
TGCACGTGAGGAATATTGCA+AGG 0.626270 5_1:-13831849 Msa0719080:three_prime_UTR
CGACTTTGTTGACAGCGTAG+AGG 0.628718 5_1:-13831707 Msa0719080:three_prime_UTR
TTATGGGCTGAATCGGCTGG+GGG 0.634285 5_1:-13832050 Msa0719080:CDS
ACAAAACATAAAACATAGTG+CGG 0.635285 5_1:+13831653 None:intergenic
AGTGTTATTTCCATCAAGGG+TGG 0.645266 5_1:-13831778 Msa0719080:three_prime_UTR
CGATTTGGAGAGACAACTGA+AGG 0.647490 5_1:-13831812 Msa0719080:three_prime_UTR
GGCATCTGATAATGGCACCA+AGG 0.663152 5_1:-13832099 Msa0719080:CDS
TGTAAATCAGATGAATGAAG+GGG 0.684019 5_1:-13832234 Msa0719080:CDS
ATCTGATAATGGCACCAAGG+AGG 0.695887 5_1:-13832096 Msa0719080:CDS
GTGATGACGCTACAACACCA+TGG 0.716250 5_1:-13832265 Msa0719080:CDS
GTTGTAGCGTCATCACTGAG+AGG 0.758690 5_1:+13832272 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTTTATGAATATGAT+AGG - chr5_1:13831626-13831645 Msa0719080:three_prime_UTR 10.0%
!! ATATCATTAAATAATAACAA+TGG + chr5_1:13832622-13832641 None:intergenic 10.0%
!! ATATTAATGTTTATTTCATT+CGG - chr5_1:13834417-13834436 Msa0719080:intron 10.0%
!! AATAACATAATCTATAAAGA+TGG + chr5_1:13832500-13832519 None:intergenic 15.0%
!! TCTTTATAGATTATGTTATT+TGG - chr5_1:13832500-13832519 Msa0719080:intron 15.0%
!!! CTTTATAGATTATGTTATTT+GGG - chr5_1:13832501-13832520 Msa0719080:intron 15.0%
!!! TTTTGACAAATAATTATATC+AGG - chr5_1:13832723-13832742 Msa0719080:intron 15.0%
!! ATATATTTATTACTAAACAC+AGG + chr5_1:13833404-13833423 None:intergenic 15.0%
!!! CTATATTTTCTTATGTTTTT+TGG - chr5_1:13833609-13833628 Msa0719080:intron 15.0%
!!! TATACTACAGTATTGAATAA+AGG - chr5_1:13831584-13831603 Msa0719080:three_prime_UTR 20.0%
!!! TGTGATTTATTTTTGGATTT+GGG - chr5_1:13831672-13831691 Msa0719080:three_prime_UTR 20.0%
!!! AAAGTTGGATTTTTTTGATT+TGG - chr5_1:13831772-13831791 Msa0719080:three_prime_UTR 20.0%
!!! AAGTTGGATTTTTTTGATTT+GGG - chr5_1:13831773-13831792 Msa0719080:three_prime_UTR 20.0%
!!! TGATAATTATCATGGTTTTA+CGG - chr5_1:13831907-13831926 Msa0719080:exon 20.0%
!! ATATCACTTGAGTAAAAATA+TGG + chr5_1:13832978-13832997 None:intergenic 20.0%
!!! TGATTTATGTGTTTTTGTTT+TGG - chr5_1:13833473-13833492 Msa0719080:intron 20.0%
!! TTTATTTCATTCGGAAAATT+GGG - chr5_1:13834426-13834445 Msa0719080:intron 20.0%
! CCAAAAATAAATCACAGATA+GGG + chr5_1:13831668-13831687 None:intergenic 25.0%
! TCCAAAAATAAATCACAGAT+AGG + chr5_1:13831669-13831688 None:intergenic 25.0%
!!! CTGTGATTTATTTTTGGATT+TGG - chr5_1:13831671-13831690 Msa0719080:three_prime_UTR 25.0%
!!! TTATTTTTGGATTTGGGTTT+TGG - chr5_1:13831678-13831697 Msa0719080:three_prime_UTR 25.0%
!!! GATTTGTGGTTTTTAAAAGT+TGG - chr5_1:13831757-13831776 Msa0719080:three_prime_UTR 25.0%
!!! GGTTAGTTTGATAATTATCA+TGG - chr5_1:13831899-13831918 Msa0719080:exon 25.0%
!!! GATAATTATCATGGTTTTAC+GGG - chr5_1:13831908-13831927 Msa0719080:CDS 25.0%
! TTGTAAGAAACATATCATGA+AGG + chr5_1:13831972-13831991 None:intergenic 25.0%
!! TGTTCATTTTCAATCACTAA+GGG - chr5_1:13832061-13832080 Msa0719080:CDS 25.0%
! ATAGCGGTTTAATGTAAAAA+TGG - chr5_1:13832137-13832156 Msa0719080:CDS 25.0%
! GTTATAACTTTCTTAATGCA+TGG - chr5_1:13832174-13832193 Msa0719080:CDS 25.0%
! ATTTCAGTTAAAGATCATCA+TGG + chr5_1:13832252-13832271 None:intergenic 25.0%
!! ATCTTTTCTTTATCTGATTC+AGG - chr5_1:13832411-13832430 Msa0719080:intron 25.0%
! ATATTGCACATATATAGACT+AGG - chr5_1:13832638-13832657 Msa0719080:intron 25.0%
! ACATATATAGACTAGGTATA+TGG - chr5_1:13832645-13832664 Msa0719080:intron 25.0%
!!! AATTTAGTTCTTACAATGCT+TGG - chr5_1:13832670-13832689 Msa0719080:intron 25.0%
!! TTGATTATGCCTTTATTTGA+TGG - chr5_1:13832746-13832765 Msa0719080:intron 25.0%
! TAAATAACAACCATTTCACT+TGG - chr5_1:13832857-13832876 Msa0719080:intron 25.0%
! AAATAACAACCATTTCACTT+GGG - chr5_1:13832858-13832877 Msa0719080:intron 25.0%
! ATAGAAACTACTACCAAATT+CGG + chr5_1:13832940-13832959 None:intergenic 25.0%
! TTGAGTAAAAATATGGTATC+AGG + chr5_1:13832971-13832990 None:intergenic 25.0%
!!! TTATGTGTTTTTGTTTTGGT+TGG - chr5_1:13833477-13833496 Msa0719080:intron 25.0%
! TTAGAGCAATAATTAGTGTA+TGG + chr5_1:13833536-13833555 None:intergenic 25.0%
!!! TATGCAAACTTTAAAACAGA+TGG + chr5_1:13833563-13833582 None:intergenic 25.0%
! ATGTAAATCAGATGAATGAA+GGG - chr5_1:13833790-13833809 Msa0719080:intron 25.0%
! TGCAACTCAATTGAAATTAT+GGG - chr5_1:13833959-13833978 Msa0719080:intron 25.0%
! ACAAAACATAAAACATAGTG+CGG + chr5_1:13834375-13834394 None:intergenic 25.0%
! GTTTATTTCATTCGGAAAAT+TGG - chr5_1:13834425-13834444 Msa0719080:intron 25.0%
! TTATTTCATTCGGAAAATTG+GGG - chr5_1:13834427-13834446 Msa0719080:intron 25.0%
!! CCCTATCTGTGATTTATTTT+TGG - chr5_1:13831665-13831684 Msa0719080:three_prime_UTR 30.0%
!! GTCAAGTAGTGTTGAATTTT+GGG - chr5_1:13831714-13831733 Msa0719080:three_prime_UTR 30.0%
!! TCAAGTAGTGTTGAATTTTG+GGG - chr5_1:13831715-13831734 Msa0719080:three_prime_UTR 30.0%
AGATTAGGACACATTCTATT+AGG - chr5_1:13831802-13831821 Msa0719080:three_prime_UTR 30.0%
GAATATTGATGAGACTTTGA+AGG - chr5_1:13831836-13831855 Msa0719080:three_prime_UTR 30.0%
! CTGTTCATTTTCAATCACTA+AGG - chr5_1:13832060-13832079 Msa0719080:CDS 30.0%
TTCACTTTGCAAATTGCAAT+TGG + chr5_1:13832304-13832323 None:intergenic 30.0%
!!! AGATGTAACTTAGTTTTGTC+TGG - chr5_1:13832375-13832394 Msa0719080:CDS 30.0%
TAATCTATAAAGATGGTGAC+TGG + chr5_1:13832493-13832512 None:intergenic 30.0%
TTGCACTTTCCATCAAATAA+AGG + chr5_1:13832758-13832777 None:intergenic 30.0%
GCTTTCATATGTTGAATTCT+AGG - chr5_1:13832820-13832839 Msa0719080:intron 30.0%
ATGAATGATTGAAGTCCTTA+AGG - chr5_1:13833500-13833519 Msa0719080:intron 30.0%
!!! TATGTTTTTTGGACAGATCA+TGG - chr5_1:13833620-13833639 Msa0719080:intron 30.0%
GATGTAAATCAGATGAATGA+AGG - chr5_1:13833789-13833808 Msa0719080:intron 30.0%
TGTAAATCAGATGAATGAAG+GGG - chr5_1:13833791-13833810 Msa0719080:intron 30.0%
TCTTGAGTAGGAAACATATA+AGG - chr5_1:13833886-13833905 Msa0719080:intron 30.0%
! CATAATTTCAATTGAGTTGC+AGG + chr5_1:13833960-13833979 None:intergenic 30.0%
CTGCAACTCAATTGAAATTA+TGG - chr5_1:13833958-13833977 Msa0719080:intron 30.0%
! ATTGAAATTATGGGCTGAAT+CGG - chr5_1:13833968-13833987 Msa0719080:intron 30.0%
TCTTTATGGTACTTGTGATA+TGG - chr5_1:13834013-13834032 Msa0719080:intron 30.0%
!!! GGGTTTTGGTTGATTTGATT+TGG - chr5_1:13831692-13831711 Msa0719080:three_prime_UTR 35.0%
!! GGTCAAGTAGTGTTGAATTT+TGG - chr5_1:13831713-13831732 Msa0719080:three_prime_UTR 35.0%
!! TTGAATTTTGGGGATCTGAA+GGG - chr5_1:13831725-13831744 Msa0719080:three_prime_UTR 35.0%
GAAGGATGCTGTGATTATAT+TGG - chr5_1:13831854-13831873 Msa0719080:three_prime_UTR 35.0%
TCACTTTGATCAATATCGTC+AGG - chr5_1:13831878-13831897 Msa0719080:three_prime_UTR 35.0%
TCACTAAGGGTCTAAACTAT+AGG - chr5_1:13832074-13832093 Msa0719080:CDS 35.0%
CACTAAGGGTCTAAACTATA+GGG - chr5_1:13832075-13832094 Msa0719080:CDS 35.0%
GTCGAATGCTCTAATTATAG+CGG - chr5_1:13832121-13832140 Msa0719080:CDS 35.0%
!! TGCAGTCAAGTTGACTAAAT+GGG + chr5_1:13832345-13832364 None:intergenic 35.0%
!! ATGCAGTCAAGTTGACTAAA+TGG + chr5_1:13832346-13832365 None:intergenic 35.0%
ATAAAGATGGTGACTGGTAA+GGG + chr5_1:13832487-13832506 None:intergenic 35.0%
TATAAAGATGGTGACTGGTA+AGG + chr5_1:13832488-13832507 None:intergenic 35.0%
CAATGCTTGGAAAATCTTTC+TGG - chr5_1:13832683-13832702 Msa0719080:intron 35.0%
ACTACTACCAAATTCGGAAA+AGG + chr5_1:13832934-13832953 None:intergenic 35.0%
ATTAGCACTACAAGCTCAAT+TGG + chr5_1:13833343-13833362 None:intergenic 35.0%
CTGAAGCATAAGAACAAGTA+TGG + chr5_1:13833373-13833392 None:intergenic 35.0%
ATTACTAAACACAGGCTCAA+GGG + chr5_1:13833396-13833415 None:intergenic 35.0%
TATTACTAAACACAGGCTCA+AGG + chr5_1:13833397-13833416 None:intergenic 35.0%
AGATGAGATGAAATCGACAT+AGG - chr5_1:13833449-13833468 Msa0719080:intron 35.0%
! TTGACCTTACGGTTTTCTTT+AGG + chr5_1:13833648-13833667 None:intergenic 35.0%
AAAGAAAACCGTAAGGTCAA+AGG - chr5_1:13833648-13833667 Msa0719080:intron 35.0%
!!! ATCATACGCTTTTTCATCTG+TGG + chr5_1:13833675-13833694 None:intergenic 35.0%
!! GTGTTTGTTTTCTCTTGAGT+AGG - chr5_1:13833874-13833893 Msa0719080:intron 35.0%
GGAAACATATAAGGAGAAGA+TGG - chr5_1:13833895-13833914 Msa0719080:intron 35.0%
ATATAAGGAGAAGATGGAGA+AGG - chr5_1:13833901-13833920 Msa0719080:intron 35.0%
!! TGAGAGTGTTATTTCCATCA+AGG - chr5_1:13834243-13834262 Msa0719080:intron 35.0%
!! GAGAGTGTTATTTCCATCAA+GGG - chr5_1:13834244-13834263 Msa0719080:intron 35.0%
!! CAGATAGGGCTTTGAAAAAC+AGG + chr5_1:13831654-13831673 None:intergenic 40.0%
!! GTTGAATTTTGGGGATCTGA+AGG - chr5_1:13831724-13831743 Msa0719080:three_prime_UTR 40.0%
!!! AAGGGTGTTTTGTGGATTTG+TGG - chr5_1:13831743-13831762 Msa0719080:three_prime_UTR 40.0%
! TGATTTGGGTGTTGCAGATT+AGG - chr5_1:13831787-13831806 Msa0719080:three_prime_UTR 40.0%
TACCTTGAGAGACCTAATAG+AGG - chr5_1:13832099-13832118 Msa0719080:CDS 40.0%
! TAGAGCATTCGACCTCTATT+AGG + chr5_1:13832114-13832133 None:intergenic 40.0%
ATGATAGTAGTCAGCATTGC+AGG + chr5_1:13832216-13832235 None:intergenic 40.0%
!! TCAAGTTGACTAAATGGGAC+TGG + chr5_1:13832340-13832359 None:intergenic 40.0%
! ATTACAGAAGCGCATGAGTT+AGG + chr5_1:13832544-13832563 None:intergenic 40.0%
! GAGATTTTGCCCAAGTGAAA+TGG + chr5_1:13832870-13832889 None:intergenic 40.0%
! CGACATTCCTTTTCCGAATT+TGG - chr5_1:13832924-13832943 Msa0719080:intron 40.0%
TGTGCATTCAGCACTACTAA+AGG - chr5_1:13833032-13833051 Msa0719080:intron 40.0%
GTTTCACAACAAGCACACTA+AGG - chr5_1:13833159-13833178 Msa0719080:intron 40.0%
AGGAACAGAGGATTTCTCTT+TGG - chr5_1:13833179-13833198 Msa0719080:intron 40.0%
! CAGAGGATTTCTCTTTGGAA+AGG - chr5_1:13833184-13833203 Msa0719080:intron 40.0%
TATGGCGTACATGTTCCTTA+AGG + chr5_1:13833518-13833537 None:intergenic 40.0%
GTCGCTTGTTTCAACTTATG+CGG + chr5_1:13833702-13833721 None:intergenic 40.0%
!! AGAAGGAAGGCATCTGATAA+TGG - chr5_1:13833918-13833937 Msa0719080:intron 40.0%
!! AAGACTCTGGGATGTCTTTA+TGG - chr5_1:13833999-13834018 Msa0719080:intron 40.0%
GCAGAAACTTGGCTACTATA+TGG + chr5_1:13834091-13834110 None:intergenic 40.0%
TATAGTAGCCAAGTTTCTGC+CGG - chr5_1:13834091-13834110 Msa0719080:intron 40.0%
GCTAATAAGCACACACGATT+TGG - chr5_1:13834198-13834217 Msa0719080:CDS 40.0%
!! AGTGTTATTTCCATCAAGGG+TGG - chr5_1:13834247-13834266 Msa0719080:intron 40.0%
GACCTCTATTAGGTCTCTCA+AGG + chr5_1:13832104-13832123 None:intergenic 45.0%
AAGAGCGAAAGCTCTTACAC+AGG - chr5_1:13832798-13832817 Msa0719080:intron 45.0%
ACAAGCACACTAAGGAACAG+AGG - chr5_1:13833167-13833186 Msa0719080:intron 45.0%
GGCTCCTAAAGAAAACCGTA+AGG - chr5_1:13833641-13833660 Msa0719080:intron 45.0%
TCTGTGGACCTTTGACCTTA+CGG + chr5_1:13833659-13833678 None:intergenic 45.0%
TGATTTACATCCCACTGCCA+TGG + chr5_1:13833780-13833799 None:intergenic 45.0%
ATCAGATGAATGAAGGGGAG+CGG - chr5_1:13833796-13833815 Msa0719080:intron 45.0%
AAGGAGAAGATGGAGAAGGA+AGG - chr5_1:13833905-13833924 Msa0719080:intron 45.0%
!! ATCTGATAATGGCACCAAGG+AGG - chr5_1:13833929-13833948 Msa0719080:intron 45.0%
! AAATTATGGGCTGAATCGGC+TGG - chr5_1:13833972-13833991 Msa0719080:intron 45.0%
! AATTATGGGCTGAATCGGCT+GGG - chr5_1:13833973-13833992 Msa0719080:intron 45.0%
TGCACGTGAGGAATATTGCA+AGG - chr5_1:13834176-13834195 Msa0719080:CDS 45.0%
! CGATTTGGAGAGACAACTGA+AGG - chr5_1:13834213-13834232 Msa0719080:CDS 45.0%
GGAGAGACAACTGAAGGAAT+TGG - chr5_1:13834219-13834238 Msa0719080:CDS 45.0%
AGACGTCATCATAGTCTGGA+TGG + chr5_1:13834285-13834304 None:intergenic 45.0%
TCAGAGACGTCATCATAGTC+TGG + chr5_1:13834289-13834308 None:intergenic 45.0%
CTCTACGCTGTCAACAAAGT+CGG + chr5_1:13834320-13834339 None:intergenic 45.0%
!!! GGGATCTGAAGGGTGTTTTG+TGG - chr5_1:13831735-13831754 Msa0719080:three_prime_UTR 50.0%
! TGCAATAGCTCTGCCAGTTC+AGG - chr5_1:13832773-13832792 Msa0719080:intron 50.0%
! GCAATAGCTCTGCCAGTTCA+GGG - chr5_1:13832774-13832793 Msa0719080:intron 50.0%
CTTTCGCTCTTTCCCTGAAC+TGG + chr5_1:13832789-13832808 None:intergenic 50.0%
GTTGTAGCGTCATCACTGAG+AGG + chr5_1:13833756-13833775 None:intergenic 50.0%
! GTGATGACGCTACAACACCA+TGG - chr5_1:13833760-13833779 Msa0719080:intron 50.0%
TCAGATGAATGAAGGGGAGC+GGG - chr5_1:13833797-13833816 Msa0719080:intron 50.0%
ATGCAGGGCAGCTGGATATA+GGG - chr5_1:13833835-13833854 Msa0719080:intron 50.0%
!! GGCATCTGATAATGGCACCA+AGG - chr5_1:13833926-13833945 Msa0719080:intron 50.0%
AGTTGCAGGATCAACCTCCT+TGG + chr5_1:13833946-13833965 None:intergenic 50.0%
! ATTATGGGCTGAATCGGCTG+GGG - chr5_1:13833974-13833993 Msa0719080:intron 50.0%
ATACATGCGACTGCTGGGTA+GGG + chr5_1:13834066-13834085 None:intergenic 50.0%
TGGAGATACATGCGACTGCT+GGG + chr5_1:13834071-13834090 None:intergenic 50.0%
ATGGAGATACATGCGACTGC+TGG + chr5_1:13834072-13834091 None:intergenic 50.0%
AGAGAGAGCAAATGCAGCAG+AGG - chr5_1:13834140-13834159 Msa0719080:intron 50.0%
GCTGTCAACAAAGTCGGCAT+CGG + chr5_1:13834314-13834333 None:intergenic 50.0%
CGACTTTGTTGACAGCGTAG+AGG - chr5_1:13834318-13834337 Msa0719080:intron 50.0%
ACGCTACAACACCATGGCAG+TGG - chr5_1:13833766-13833785 Msa0719080:intron 55.0%
CGCTACAACACCATGGCAGT+GGG - chr5_1:13833767-13833786 Msa0719080:intron 55.0%
GATGCAGGGCAGCTGGATAT+AGG - chr5_1:13833834-13833853 Msa0719080:intron 55.0%
! TTATGGGCTGAATCGGCTGG+GGG - chr5_1:13833975-13833994 Msa0719080:intron 55.0%
! ATGGCAAAGCGCTACAAGCC+CGG - chr5_1:13834032-13834051 Msa0719080:intron 55.0%
GATACATGCGACTGCTGGGT+AGG + chr5_1:13834067-13834086 None:intergenic 55.0%
GCAGCAATCCGGCAGAAACT+TGG + chr5_1:13834102-13834121 None:intergenic 55.0%
CTGCCATCTGTGCAGCAATC+CGG + chr5_1:13834113-13834132 None:intergenic 55.0%
! TGATGCTCGAGTTGCACGTG+AGG - chr5_1:13834164-13834183 Msa0719080:CDS 55.0%
AGTCTGGATGGCGACGATGT+CGG + chr5_1:13834273-13834292 None:intergenic 55.0%
AATGAAGGGGAGCGGGATGC+AGG - chr5_1:13833804-13833823 Msa0719080:intron 60.0%
ATGAAGGGGAGCGGGATGCA+GGG - chr5_1:13833805-13833824 Msa0719080:intron 60.0%
ATGCAGGGGAGCGAGATGCA+GGG - chr5_1:13833820-13833839 Msa0719080:intron 60.0%
ATCGGCTGGGGGAAAGACTC+TGG - chr5_1:13833986-13834005 Msa0719080:intron 60.0%
TCGGCTGGGGGAAAGACTCT+GGG - chr5_1:13833987-13834006 Msa0719080:intron 60.0%
CTGGGTAGGGATGATGCACC+GGG + chr5_1:13834053-13834072 None:intergenic 60.0%
GCTGGGTAGGGATGATGCAC+CGG + chr5_1:13834054-13834073 None:intergenic 60.0%
CTGCCGGATTGCTGCACAGA+TGG - chr5_1:13834107-13834126 Msa0719080:intron 60.0%
ACGATGTCGGCCACCCTTGA+TGG + chr5_1:13834260-13834279 None:intergenic 60.0%
TGAAGGGGAGCGGGATGCAG+GGG - chr5_1:13833806-13833825 Msa0719080:intron 65.0%
GATGCAGGGGAGCGAGATGC+AGG - chr5_1:13833819-13833838 Msa0719080:intron 65.0%
GGAGCGAGATGCAGGGCAGC+TGG - chr5_1:13833827-13833846 Msa0719080:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 13831556 13834491 13831556 ID=Msa0719080;Name=Msa0719080
chr5_1 mRNA 13831556 13834491 13831556 ID=Msa0719080-mRNA-1;Parent=Msa0719080;Name=Msa0719080-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0.33|0.5|0.5|1|1|4|352|203
chr5_1 exon 13831556 13832411 13831556 ID=Msa0719080-mRNA-1:exon:5544;Parent=Msa0719080-mRNA-1
chr5_1 exon 13834148 13834243 13834148 ID=Msa0719080-mRNA-1:exon:5543;Parent=Msa0719080-mRNA-1
chr5_1 exon 13834394 13834399 13834394 ID=Msa0719080-mRNA-1:exon:5542;Parent=Msa0719080-mRNA-1
chr5_1 exon 13834486 13834491 13834486 ID=Msa0719080-mRNA-1:exon:5541;Parent=Msa0719080-mRNA-1
chr5_1 CDS 13834486 13834491 13834486 ID=Msa0719080-mRNA-1:cds;Parent=Msa0719080-mRNA-1
chr5_1 CDS 13834394 13834399 13834394 ID=Msa0719080-mRNA-1:cds;Parent=Msa0719080-mRNA-1
chr5_1 CDS 13834148 13834243 13834148 ID=Msa0719080-mRNA-1:cds;Parent=Msa0719080-mRNA-1
chr5_1 CDS 13831908 13832411 13831908 ID=Msa0719080-mRNA-1:cds;Parent=Msa0719080-mRNA-1
chr5_1 three_prime_UTR 13831556 13831907 13831556 ID=Msa0719080-mRNA-1:three_prime_utr;Parent=Msa0719080-mRNA-1
Gene Sequence

>Msa0719080

ATGAAAGTACCTATTAGGACACATTCTATTAGGAAAGCTCATGAGAATATTGATGAGACTTTGAAGGATGCTGTGATTATATTGGCTCACTTTGATCAATATCGTCAGATCATGGCTCCTAAAGAAAACCGTAAGGTCAAAGGTCCACAGATGAAAAAGCGTATGATTATGCCGCATAAGTTGAAACAAGCGACTAAGCATCTACATGCATCAGCTGTGAAGTCACCTCTCAGTGATGACGCTACAACACCATGGCAGTGGGATGTAAATCAGATGAATGAAGGGGAGCGGGATGCAGGGGAGCGAGATGCAGGGCAGCTGGATATAGGGCATCATATAAAAATGTGTGTTTGTTTTCTCTTGAGTAGGAAACATATAAGGAGAAGATGGAGAAGGAAGGCATCTGATAATGGCACCAAGGAGGTTGATCCTGCAACTCAATTGAAATTATGGGCTGAATCGGCTGGGGGAAAGACTCTGGGATGTCTTTATGGTACTTGTGATATGGCAAAGCGCTACAAGCCCGGTGCATCATCCCTACCCAGCAGTCGCATGTATCTCCATATAGTAGCCAAGTTTCTGCCGGATTGCTGCACAGATGGCAGCAGTTGA
Protein sequence

>Msa0719080

MKVPIRTHSIRKAHENIDETLKDAVIILAHFDQYRQIMAPKENRKVKGPQMKKRMIMPHKLKQATKHLHASAVKSPLSDDATTPWQWDVNQMNEGERDAGERDAGQLDIGHHIKMCVCFLLSRKHIRRRWRRKASDNGTKEVDPATQLKLWAESAGGKTLGCLYGTCDMAKRYKPGASSLPSSRMYLHIVAKFLPDCCTDGSS*