AlfalfaGEDB | Msa0719080

M. sativa cultivar zhongmu-4 / Msa0719080

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719080	RHN69474.1	46.875	96	31	3	38	132	1	77	8.98e-12	69.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719080	A0A396IYI1	46.875	96	31	3	38	132	1	77	4.29e-12	69.3

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010260	Msa0719080	0.802020	6.838946e-49	-8.615850e-47
Msa0063040	Msa0719080	0.810397	1.176590e-50	-8.615850e-47
Msa0213670	Msa0719080	0.811616	6.403480e-51	-8.615850e-47
Msa0242320	Msa0719080	0.804298	2.310725e-49	-8.615850e-47
Msa0269940	Msa0719080	0.823580	1.283334e-53	-8.615850e-47
Msa0427350	Msa0719080	0.835307	1.811137e-56	-8.615850e-47
Msa0439160	Msa0719080	0.804491	2.106203e-49	-8.615850e-47
Msa0500520	Msa0719080	0.875725	2.457382e-68	-8.615850e-47
Msa0500530	Msa0719080	0.830827	2.358745e-55	-8.615850e-47
Msa0500540	Msa0719080	0.822097	2.841866e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719080	MtrunA17_Chr3g0125141	46.875	96	31	3	38	132	1	77	8.26e-16	69.3
Msa0719080	MtrunA17_Chr1g0197101	71.111	45	9	1	5	49	55	95	5.17e-12	63.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 78 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAAACCGTAAGGTCAA+AGG	0.566284	5_1:-13832377	Msa0719080:CDS
AAATTATGGGCTGAATCGGC+TGG	0.391995	5_1:-13832053	Msa0719080:CDS
AAGACTCTGGGATGTCTTTA+TGG	0.210146	5_1:-13832026	Msa0719080:CDS
AAGGAGAAGATGGAGAAGGA+AGG	0.533403	5_1:-13832120	Msa0719080:CDS
AATAACTTTCATAACTGCAA+TGG	0.524765	5_1:+13831542	None:intergenic
AATGAAGGGGAGCGGGATGC+AGG	0.385018	5_1:-13832221	Msa0719080:CDS
AATTATGGGCTGAATCGGCT+GGG	0.505977	5_1:-13832052	Msa0719080:CDS
ACAAAACATAAAACATAGTG+CGG	0.635285	5_1:+13831653	None:intergenic
ACGATGTCGGCCACCCTTGA+TGG	0.373602	5_1:+13831768	None:intergenic
ACGCTACAACACCATGGCAG+TGG	0.545516	5_1:-13832259	Msa0719080:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATTAGGACACATTCTATT+AGG	-	chr5_1:13831802-13831821	Msa0719080:three_prime_UTR	30.0%
GAATATTGATGAGACTTTGA+AGG	-	chr5_1:13831836-13831855	Msa0719080:three_prime_UTR	30.0%
TTCACTTTGCAAATTGCAAT+TGG	+	chr5_1:13832304-13832323	None:intergenic	30.0%
TAATCTATAAAGATGGTGAC+TGG	+	chr5_1:13832493-13832512	None:intergenic	30.0%
TTGCACTTTCCATCAAATAA+AGG	+	chr5_1:13832758-13832777	None:intergenic	30.0%
GCTTTCATATGTTGAATTCT+AGG	-	chr5_1:13832820-13832839	Msa0719080:intron	30.0%
ATGAATGATTGAAGTCCTTA+AGG	-	chr5_1:13833500-13833519	Msa0719080:intron	30.0%
GATGTAAATCAGATGAATGA+AGG	-	chr5_1:13833789-13833808	Msa0719080:intron	30.0%
TGTAAATCAGATGAATGAAG+GGG	-	chr5_1:13833791-13833810	Msa0719080:intron	30.0%
TCTTGAGTAGGAAACATATA+AGG	-	chr5_1:13833886-13833905	Msa0719080:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	13831556	13834491	13831556	ID=Msa0719080;Name=Msa0719080
chr5_1	mRNA	13831556	13834491	13831556	ID=Msa0719080-mRNA-1;Parent=Msa0719080;Name=Msa0719080-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0.33\|0.5\|0.5\|1\|1\|4\|352\|203
chr5_1	exon	13831556	13832411	13831556	ID=Msa0719080-mRNA-1:exon:5544;Parent=Msa0719080-mRNA-1
chr5_1	exon	13834148	13834243	13834148	ID=Msa0719080-mRNA-1:exon:5543;Parent=Msa0719080-mRNA-1
chr5_1	exon	13834394	13834399	13834394	ID=Msa0719080-mRNA-1:exon:5542;Parent=Msa0719080-mRNA-1
chr5_1	exon	13834486	13834491	13834486	ID=Msa0719080-mRNA-1:exon:5541;Parent=Msa0719080-mRNA-1
chr5_1	CDS	13834486	13834491	13834486	ID=Msa0719080-mRNA-1:cds;Parent=Msa0719080-mRNA-1
chr5_1	CDS	13834394	13834399	13834394	ID=Msa0719080-mRNA-1:cds;Parent=Msa0719080-mRNA-1
chr5_1	CDS	13834148	13834243	13834148	ID=Msa0719080-mRNA-1:cds;Parent=Msa0719080-mRNA-1
chr5_1	CDS	13831908	13832411	13831908	ID=Msa0719080-mRNA-1:cds;Parent=Msa0719080-mRNA-1
chr5_1	three_prime_UTR	13831556	13831907	13831556	ID=Msa0719080-mRNA-1:three_prime_utr;Parent=Msa0719080-mRNA-1

Gene Sequence

>Msa0719080

ATGAAAGTACCTATTAGGACACATTCTATTAGGAAAGCTCATGAGAATATTGATGAGACTTTGAAGGATGCTGTGATTATATTGGCTCACTTTGATCAATATCGTCAGATCATGGCTCCTAAAGAAAACCGTAAGGTCAAAGGTCCACAGATGAAAAAGCGTATGATTATGCCGCATAAGTTGAAACAAGCGACTAAGCATCTACATGCATCAGCTGTGAAGTCACCTCTCAGTGATGACGCTACAACACCATGGCAGTGGGATGTAAATCAGATGAATGAAGGGGAGCGGGATGCAGGGGAGCGAGATGCAGGGCAGCTGGATATAGGGCATCATATAAAAATGTGTGTTTGTTTTCTCTTGAGTAGGAAACATATAAGGAGAAGATGGAGAAGGAAGGCATCTGATAATGGCACCAAGGAGGTTGATCCTGCAACTCAATTGAAATTATGGGCTGAATCGGCTGGGGGAAAGACTCTGGGATGTCTTTATGGTACTTGTGATATGGCAAAGCGCTACAAGCCCGGTGCATCATCCCTACCCAGCAGTCGCATGTATCTCCATATAGTAGCCAAGTTTCTGCCGGATTGCTGCACAGATGGCAGCAGTTGA

Protein sequence

>Msa0719080

MKVPIRTHSIRKAHENIDETLKDAVIILAHFDQYRQIMAPKENRKVKGPQMKKRMIMPHKLKQATKHLHASAVKSPLSDDATTPWQWDVNQMNEGERDAGERDAGQLDIGHHIKMCVCFLLSRKHIRRRWRRKASDNGTKEVDPATQLKLWAESAGGKTLGCLYGTCDMAKRYKPGASSLPSSRMYLHIVAKFLPDCCTDGSS*