AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0515280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515280 MtrunA17_Chr3g0124361 89.175 194 10 3 1 193 1 184 3.93e-125 351
Msa0515280 MtrunA17_Chr3g0115551 33.898 177 74 7 1 177 1 134 2.05e-21 86.3
Msa0515280 MtrunA17_Chr8g0336591 53.571 56 25 1 127 181 32 87 2.14e-15 69.3
Msa0515280 MtrunA17_Chr4g0055191 61.702 47 18 0 131 177 71 117 5.80e-15 69.3
Msa0515280 MtrunA17_Chr5g0400051 50.704 71 29 2 131 196 83 152 1.38e-14 68.6
Msa0515280 MtrunA17_Chr8g0344991 44.286 70 36 1 133 202 39 105 6.96e-14 65.5
Msa0515280 MtrunA17_Chr4g0070221 54.902 51 22 1 127 177 172 221 9.15e-12 62.4
Msa0515280 MtrunA17_Chr2g0295651 58.537 41 17 0 133 173 64 104 9.79e-12 60.5
Msa0515280 MtrunA17_Chr2g0301141 40.741 81 45 2 101 179 152 231 1.57e-11 61.6
Msa0515280 MtrunA17_Chr4g0067541 43.548 62 34 1 114 175 44 104 4.42e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515280 AT1G78020.1 33.010 206 94 7 1 206 1 162 1.57e-23 92.4
Msa0515280 AT1G22160.1 35.196 179 61 8 1 179 1 124 2.20e-19 81.3
Msa0515280 AT4G39795.1 45.833 72 36 1 112 183 55 123 6.08e-17 74.3
Msa0515280 AT4G17670.1 62.000 50 19 0 128 177 71 120 1.35e-16 73.9
Msa0515280 AT5G65040.1 51.389 72 31 1 114 185 42 109 1.63e-16 72.8
Msa0515280 AT2G44670.1 62.000 50 18 1 131 179 14 63 6.14e-16 70.5
Msa0515280 AT5G47060.2 63.830 47 17 0 131 177 95 141 5.87e-15 70.1
Msa0515280 AT5G47060.1 63.830 47 17 0 131 177 95 141 5.87e-15 70.1
Msa0515280 AT1G53885.1 48.529 68 33 1 109 176 20 85 1.78e-12 62.4
Msa0515280 AT1G53903.1 48.529 68 33 1 109 176 20 85 1.78e-12 62.4
Msa0515280 AT1G19200.1 49.091 55 27 1 126 180 143 196 4.97e-11 60.1
Msa0515280 AT1G74940.1 45.902 61 32 1 132 192 149 208 5.45e-11 60.1
Msa0515280 AT5G49120.1 39.130 69 42 0 105 173 40 108 5.83e-11 58.9
Msa0515280 AT1G19200.2 49.091 55 27 1 126 180 156 209 6.13e-11 60.1

Find 67 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGTTCTTCTCATAAT+TGG 0.156033 3_4:+66465024 None:intergenic
CTCAATTATTATGCTCCTTA+TGG 0.202337 3_4:-66463829 Msa0515280:three_prime_UTR
CTTCCTCATGATGGGATTCT+AGG 0.211376 3_4:+66464950 None:intergenic
ACACTTGAATAATTATTATA+AGG 0.265097 3_4:-66463980 Msa0515280:three_prime_UTR
AATAATATTGTTAGTGTTTG+TGG 0.326456 3_4:+66464738 None:intergenic
AGTTACAGAGGCCACAAGTT+TGG 0.349033 3_4:+66464650 None:intergenic
ATGATGGGATTCTAGGTTGT+TGG 0.366587 3_4:+66464957 None:intergenic
ACAAGCAGCAGTAGAAGCTT+TGG 0.372677 3_4:+66464020 None:intergenic
TCTAGGTTGTTGGTTTGATC+AGG 0.381479 3_4:+66464967 None:intergenic
TACTAGAAACTTTGTAAAGA+AGG 0.385710 3_4:-66463759 Msa0515280:three_prime_UTR
ATTGTTAGTGTTTGTGGCTG+TGG 0.394454 3_4:+66464744 None:intergenic
TGTAACTGTCGTTTGGCTCC+TGG 0.404985 3_4:-66464634 Msa0515280:CDS
TACACGGTGGTCTTCTTTGT+TGG 0.433148 3_4:+66464065 None:intergenic
ACACATTAAAAGTGAATTAA+CGG 0.437387 3_4:-66464818 Msa0515280:CDS
CTGCTGCTTGTACTTGAAGA+AGG 0.439916 3_4:-66464008 Msa0515280:exon
TGGCCTCTGTAACTGTCGTT+TGG 0.455985 3_4:-66464641 Msa0515280:CDS
CTTGTTCTTCTCATAATTGG+TGG 0.464977 3_4:+66465027 None:intergenic
CACTGATTCTTCCTCATGAT+GGG 0.470672 3_4:+66464942 None:intergenic
AGCATAATAATTGAGACAAA+GGG 0.486017 3_4:+66463837 None:intergenic
GATTAGAAGAACATGTTGCT+TGG 0.489313 3_4:-66465063 Msa0515280:exon
TCACTGATTCTTCCTCATGA+TGG 0.491322 3_4:+66464941 None:intergenic
AAGACAAAAGAAAAGAAAAG+TGG 0.499141 3_4:-66464101 Msa0515280:CDS
GTCTTGATGTTAGAAATAGT+TGG 0.501218 3_4:+66465102 None:intergenic
GTTCCCATCACAACCGTATG+TGG 0.501901 3_4:+66464847 None:intergenic
GAATCCACATACGGTTGTGA+TGG 0.509624 3_4:-66464851 Msa0515280:CDS
TGATCATCATGGTGTGGATG+TGG 0.510086 3_4:+66464895 None:intergenic
GTGGCTGTGGCTGTGTGTGA+AGG 0.511685 3_4:+66464757 None:intergenic
GAGCATAATAATTGAGACAA+AGG 0.520661 3_4:+66463836 None:intergenic
GGTGGCTGATGAACGTTTAG+TGG 0.522207 3_4:-66464797 Msa0515280:CDS
GTCGAGACATCTACATGTAT+AGG 0.522956 3_4:-66464612 Msa0515280:intron
ATCAGGTGGTTGCATGTCCA+CGG 0.524115 3_4:+66464984 None:intergenic
AAGAAAAGAAAAGTGGAAGA+TGG 0.529987 3_4:-66464094 Msa0515280:CDS
TTGTGCACTTACGACAAAGA+AGG 0.537138 3_4:-66463874 Msa0515280:three_prime_UTR
TGTGTGTGACTGAGAAGGAA+TGG 0.540588 3_4:+66465245 None:intergenic
CTTCTCATAATTGGTGGTCG+AGG 0.540724 3_4:+66465033 None:intergenic
AGGTTGTTGGTTTGATCAGG+TGG 0.542107 3_4:+66464970 None:intergenic
TGGCTGTGGCTGTGTGTGAA+GGG 0.543537 3_4:+66464758 None:intergenic
TGCCATGTTCTTGATCATCA+TGG 0.545211 3_4:+66464884 None:intergenic
AGCAGTAGAAGCTTTGGCAG+TGG 0.548192 3_4:+66464026 None:intergenic
ACCAGTGTAAAAGAGCCATA+AGG 0.561274 3_4:+66463814 None:intergenic
GCTTTGGCAGTGGCCGTGCA+CGG 0.562038 3_4:+66464036 None:intergenic
TCATGTGCACTCACACTCAA+AGG 0.576185 3_4:+66464700 None:intergenic
CCGTGTATCACCACCGTGCA+CGG 0.577796 3_4:-66464049 Msa0515280:CDS
AATCCACATACGGTTGTGAT+GGG 0.578470 3_4:-66464850 Msa0515280:CDS
CCTCACTTTCTCCAAACTTG+TGG 0.579241 3_4:-66464661 Msa0515280:CDS
TGCTGCTTGTACTTGAAGAA+GGG 0.586632 3_4:-66464007 Msa0515280:exon
CCGTGCACGGTGGTGATACA+CGG 0.602872 3_4:+66464049 None:intergenic
CACCATGATGATCAAGAACA+TGG 0.605152 3_4:-66464886 Msa0515280:CDS
TGGTGTGGATGTGGCTGCAG+AGG 0.618105 3_4:+66464904 None:intergenic
GTGGCTGATGAACGTTTAGT+GGG 0.621978 3_4:-66464796 Msa0515280:CDS
CCACAAGTTTGGAGAAAGTG+AGG 0.629285 3_4:+66464661 None:intergenic
TGTTCTTGATCATCATGGTG+TGG 0.629577 3_4:+66464889 None:intergenic
CAACATCAAGAATCCACATA+CGG 0.631823 3_4:-66464860 Msa0515280:CDS
CATGAGTGGTGGTTTGTCCG+TGG 0.637137 3_4:-66465001 Msa0515280:CDS
CATGAATGACATTGTAAGCG+TGG 0.637323 3_4:+66463907 None:intergenic
AGAAGAACAAGAAGCATGAG+TGG 0.643370 3_4:-66465015 Msa0515280:CDS
TACATGTAGATGTCTCGACC+AGG 0.645212 3_4:+66464616 None:intergenic
CAACCTAGAATCCCATCATG+AGG 0.662994 3_4:-66464953 Msa0515280:CDS
AGGGGTGTGTGTGACTGAGA+AGG 0.669343 3_4:+66465240 None:intergenic
TTGGCAGTGGCCGTGCACGG+TGG 0.682224 3_4:+66464039 None:intergenic
GAGCCAAACGACAGTTACAG+AGG 0.685876 3_4:+66464638 None:intergenic
AGAACAAGAAGCATGAGTGG+TGG 0.691730 3_4:-66465012 Msa0515280:CDS
CATTAAAAGTGAATTAACGG+TGG 0.697236 3_4:-66464815 Msa0515280:CDS
AGACAAAGGGAGAAACGAGG+TGG 0.714534 3_4:+66463850 None:intergenic
GTGTGACTGAGAAGGAATGG+TGG 0.728901 3_4:+66465248 None:intergenic
TGCACGGTGGTGATACACGG+TGG 0.754526 3_4:+66464052 None:intergenic
TTGAGACAAAGGGAGAAACG+AGG 0.774479 3_4:+66463847 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGAATAATAGAAAAAAA+TGG + chr3_4:66464321-66464340 None:intergenic 10.0%
!!! TTAATTAATTTTTTGTTTAG+AGG - chr3_4:66464653-66464672 Msa0515280:CDS 10.0%
!! TAATGAATAATAGAAAAAAA+TGG + chr3_4:66464321-66464340 None:intergenic 10.0%
!!! TTAATTAATTTTTTGTTTAG+AGG - chr3_4:66464653-66464672 Msa0515280:CDS 10.0%
!! GTTAGTTAGTATAATTAATT+TGG - chr3_4:66464684-66464703 Msa0515280:CDS 15.0%
!!! AAAGAAAATTATTGATTTCA+GGG - chr3_4:66464728-66464747 Msa0515280:CDS 15.0%
!! ACACTTGAATAATTATTATA+AGG - chr3_4:66464920-66464939 Msa0515280:CDS 15.0%
!! GTTAGTTAGTATAATTAATT+TGG - chr3_4:66464684-66464703 Msa0515280:CDS 15.0%
!!! AAAGAAAATTATTGATTTCA+GGG - chr3_4:66464728-66464747 Msa0515280:CDS 15.0%
!! ACACTTGAATAATTATTATA+AGG - chr3_4:66464920-66464939 Msa0515280:CDS 15.0%
!! ACACATTAAAAGTGAATTAA+CGG - chr3_4:66464082-66464101 Msa0515280:CDS 20.0%
!! AATAATATTGTTAGTGTTTG+TGG + chr3_4:66464165-66464184 None:intergenic 20.0%
!! GTAAATATTAATTCTTCCTT+TGG + chr3_4:66464547-66464566 None:intergenic 20.0%
!!! GAAAGAAAATTATTGATTTC+AGG - chr3_4:66464727-66464746 Msa0515280:CDS 20.0%
!!! AAGAAAATTATTGATTTCAG+GGG - chr3_4:66464729-66464748 Msa0515280:CDS 20.0%
!! ACACATTAAAAGTGAATTAA+CGG - chr3_4:66464082-66464101 Msa0515280:CDS 20.0%
!! AATAATATTGTTAGTGTTTG+TGG + chr3_4:66464165-66464184 None:intergenic 20.0%
!! GTAAATATTAATTCTTCCTT+TGG + chr3_4:66464547-66464566 None:intergenic 20.0%
!!! GAAAGAAAATTATTGATTTC+AGG - chr3_4:66464727-66464746 Msa0515280:CDS 20.0%
!!! AAGAAAATTATTGATTTCAG+GGG - chr3_4:66464729-66464748 Msa0515280:CDS 20.0%
!! TGACTTTGATATTCTTGTTT+AGG + chr3_4:66464428-66464447 None:intergenic 25.0%
! TAAACAAGAATATCAAAGTC+AGG - chr3_4:66464427-66464446 Msa0515280:intron 25.0%
! CTGAAAATAAAGAGACAATT+AGG + chr3_4:66464517-66464536 None:intergenic 25.0%
!!! TTTTTGTTTAGAGGAAATTG+TGG - chr3_4:66464662-66464681 Msa0515280:CDS 25.0%
! AAGACAAAAGAAAAGAAAAG+TGG - chr3_4:66464799-66464818 Msa0515280:CDS 25.0%
! AGCATAATAATTGAGACAAA+GGG + chr3_4:66465066-66465085 None:intergenic 25.0%
! TACTAGAAACTTTGTAAAGA+AGG - chr3_4:66465141-66465160 Msa0515280:five_prime_UTR 25.0%
!! TGACTTTGATATTCTTGTTT+AGG + chr3_4:66464428-66464447 None:intergenic 25.0%
! TAAACAAGAATATCAAAGTC+AGG - chr3_4:66464427-66464446 Msa0515280:intron 25.0%
! CTGAAAATAAAGAGACAATT+AGG + chr3_4:66464517-66464536 None:intergenic 25.0%
!!! TTTTTGTTTAGAGGAAATTG+TGG - chr3_4:66464662-66464681 Msa0515280:CDS 25.0%
! AAGACAAAAGAAAAGAAAAG+TGG - chr3_4:66464799-66464818 Msa0515280:CDS 25.0%
! AGCATAATAATTGAGACAAA+GGG + chr3_4:66465066-66465085 None:intergenic 25.0%
! TACTAGAAACTTTGTAAAGA+AGG - chr3_4:66465141-66465160 Msa0515280:five_prime_UTR 25.0%
GTCTTGATGTTAGAAATAGT+TGG + chr3_4:66463801-66463820 None:intergenic 30.0%
CTTCTTGTTCTTCTCATAAT+TGG + chr3_4:66463879-66463898 None:intergenic 30.0%
CATTAAAAGTGAATTAACGG+TGG - chr3_4:66464085-66464104 Msa0515280:CDS 30.0%
ACTAACCATAAGAGTTAGTT+AGG - chr3_4:66464454-66464473 Msa0515280:intron 30.0%
TTGAACCTAACTAACTCTTA+TGG + chr3_4:66464462-66464481 None:intergenic 30.0%
!! TATTTTCAGTATCGAACCAA+AGG - chr3_4:66464528-66464547 Msa0515280:intron 30.0%
TTCATTCTAGTAGTTTGACT+AGG - chr3_4:66464576-66464595 Msa0515280:intron 30.0%
AAGAAAAGAAAAGTGGAAGA+TGG - chr3_4:66464806-66464825 Msa0515280:CDS 30.0%
GAGCATAATAATTGAGACAA+AGG + chr3_4:66465067-66465086 None:intergenic 30.0%
CTCAATTATTATGCTCCTTA+TGG - chr3_4:66465071-66465090 Msa0515280:exon 30.0%
!! ACTAGCTATTTGAGTTTTAC+TGG - chr3_4:66465171-66465190 Msa0515280:five_prime_UTR 30.0%
GTCTTGATGTTAGAAATAGT+TGG + chr3_4:66463801-66463820 None:intergenic 30.0%
CTTCTTGTTCTTCTCATAAT+TGG + chr3_4:66463879-66463898 None:intergenic 30.0%
CATTAAAAGTGAATTAACGG+TGG - chr3_4:66464085-66464104 Msa0515280:CDS 30.0%
ACTAACCATAAGAGTTAGTT+AGG - chr3_4:66464454-66464473 Msa0515280:intron 30.0%
TTGAACCTAACTAACTCTTA+TGG + chr3_4:66464462-66464481 None:intergenic 30.0%
!! TATTTTCAGTATCGAACCAA+AGG - chr3_4:66464528-66464547 Msa0515280:intron 30.0%
TTCATTCTAGTAGTTTGACT+AGG - chr3_4:66464576-66464595 Msa0515280:intron 30.0%
AAGAAAAGAAAAGTGGAAGA+TGG - chr3_4:66464806-66464825 Msa0515280:CDS 30.0%
GAGCATAATAATTGAGACAA+AGG + chr3_4:66465067-66465086 None:intergenic 30.0%
CTCAATTATTATGCTCCTTA+TGG - chr3_4:66465071-66465090 Msa0515280:exon 30.0%
!! ACTAGCTATTTGAGTTTTAC+TGG - chr3_4:66465171-66465190 Msa0515280:five_prime_UTR 30.0%
TGAGTTCACACAAAAACAGA+GGG + chr3_4:66463682-66463701 None:intergenic 35.0%
TTGAGTTCACACAAAAACAG+AGG + chr3_4:66463683-66463702 None:intergenic 35.0%
GATTAGAAGAACATGTTGCT+TGG - chr3_4:66463837-66463856 Msa0515280:three_prime_UTR 35.0%
CTTGTTCTTCTCATAATTGG+TGG + chr3_4:66463876-66463895 None:intergenic 35.0%
CAACATCAAGAATCCACATA+CGG - chr3_4:66464040-66464059 Msa0515280:CDS 35.0%
AGACATCTACATGTATAGGT+AGG - chr3_4:66464292-66464311 Msa0515280:intron 35.0%
! TTTTACCTTTGACTGAACTC+CGG + chr3_4:66464401-66464420 None:intergenic 35.0%
! AGATACAGCATTTTGCAGTT+TGG - chr3_4:66464752-66464771 Msa0515280:CDS 35.0%
TGAGTTCACACAAAAACAGA+GGG + chr3_4:66463682-66463701 None:intergenic 35.0%
TTGAGTTCACACAAAAACAG+AGG + chr3_4:66463683-66463702 None:intergenic 35.0%
GATTAGAAGAACATGTTGCT+TGG - chr3_4:66463837-66463856 Msa0515280:three_prime_UTR 35.0%
CTTGTTCTTCTCATAATTGG+TGG + chr3_4:66463876-66463895 None:intergenic 35.0%
CAACATCAAGAATCCACATA+CGG - chr3_4:66464040-66464059 Msa0515280:CDS 35.0%
AGACATCTACATGTATAGGT+AGG - chr3_4:66464292-66464311 Msa0515280:intron 35.0%
! TTTTACCTTTGACTGAACTC+CGG + chr3_4:66464401-66464420 None:intergenic 35.0%
! AGATACAGCATTTTGCAGTT+TGG - chr3_4:66464752-66464771 Msa0515280:CDS 35.0%
GAGTTCACACAAAAACAGAG+GGG + chr3_4:66463681-66463700 None:intergenic 40.0%
TAGTTAGAGAGAGAGAGAGA+GGG + chr3_4:66463734-66463753 None:intergenic 40.0%
! AGAAGAACAAGAAGCATGAG+TGG - chr3_4:66463885-66463904 Msa0515280:three_prime_UTR 40.0%
!! TCTAGGTTGTTGGTTTGATC+AGG + chr3_4:66463936-66463955 None:intergenic 40.0%
! ATGATGGGATTCTAGGTTGT+TGG + chr3_4:66463946-66463965 None:intergenic 40.0%
!! CACTGATTCTTCCTCATGAT+GGG + chr3_4:66463961-66463980 None:intergenic 40.0%
!! TCACTGATTCTTCCTCATGA+TGG + chr3_4:66463962-66463981 None:intergenic 40.0%
TGTTCTTGATCATCATGGTG+TGG + chr3_4:66464014-66464033 None:intergenic 40.0%
CACCATGATGATCAAGAACA+TGG - chr3_4:66464014-66464033 Msa0515280:CDS 40.0%
TGCCATGTTCTTGATCATCA+TGG + chr3_4:66464019-66464038 None:intergenic 40.0%
AATCCACATACGGTTGTGAT+GGG - chr3_4:66464050-66464069 Msa0515280:CDS 40.0%
ATTGTTAGTGTTTGTGGCTG+TGG + chr3_4:66464159-66464178 None:intergenic 40.0%
GTCGAGACATCTACATGTAT+AGG - chr3_4:66464288-66464307 Msa0515280:intron 40.0%
!! ATTTTGCAGTTTGGAGTGCA+GGG - chr3_4:66464761-66464780 Msa0515280:CDS 40.0%
! TGCTGCTTGTACTTGAAGAA+GGG - chr3_4:66464893-66464912 Msa0515280:CDS 40.0%
CATGAATGACATTGTAAGCG+TGG + chr3_4:66464996-66465015 None:intergenic 40.0%
TTGTGCACTTACGACAAAGA+AGG - chr3_4:66465026-66465045 Msa0515280:CDS 40.0%
! TCCTTATGGCTCTTTTACAC+TGG - chr3_4:66465085-66465104 Msa0515280:five_prime_UTR 40.0%
ACCAGTGTAAAAGAGCCATA+AGG + chr3_4:66465089-66465108 None:intergenic 40.0%
GAGTTCACACAAAAACAGAG+GGG + chr3_4:66463681-66463700 None:intergenic 40.0%
TAGTTAGAGAGAGAGAGAGA+GGG + chr3_4:66463734-66463753 None:intergenic 40.0%
! AGAAGAACAAGAAGCATGAG+TGG - chr3_4:66463885-66463904 Msa0515280:three_prime_UTR 40.0%
!! TCTAGGTTGTTGGTTTGATC+AGG + chr3_4:66463936-66463955 None:intergenic 40.0%
! ATGATGGGATTCTAGGTTGT+TGG + chr3_4:66463946-66463965 None:intergenic 40.0%
!! CACTGATTCTTCCTCATGAT+GGG + chr3_4:66463961-66463980 None:intergenic 40.0%
!! TCACTGATTCTTCCTCATGA+TGG + chr3_4:66463962-66463981 None:intergenic 40.0%
TGTTCTTGATCATCATGGTG+TGG + chr3_4:66464014-66464033 None:intergenic 40.0%
CACCATGATGATCAAGAACA+TGG - chr3_4:66464014-66464033 Msa0515280:CDS 40.0%
TGCCATGTTCTTGATCATCA+TGG + chr3_4:66464019-66464038 None:intergenic 40.0%
AATCCACATACGGTTGTGAT+GGG - chr3_4:66464050-66464069 Msa0515280:CDS 40.0%
ATTGTTAGTGTTTGTGGCTG+TGG + chr3_4:66464159-66464178 None:intergenic 40.0%
GTCGAGACATCTACATGTAT+AGG - chr3_4:66464288-66464307 Msa0515280:intron 40.0%
!! ATTTTGCAGTTTGGAGTGCA+GGG - chr3_4:66464761-66464780 Msa0515280:CDS 40.0%
! TGCTGCTTGTACTTGAAGAA+GGG - chr3_4:66464893-66464912 Msa0515280:CDS 40.0%
CATGAATGACATTGTAAGCG+TGG + chr3_4:66464996-66465015 None:intergenic 40.0%
TTGTGCACTTACGACAAAGA+AGG - chr3_4:66465026-66465045 Msa0515280:CDS 40.0%
! TCCTTATGGCTCTTTTACAC+TGG - chr3_4:66465085-66465104 Msa0515280:five_prime_UTR 40.0%
ACCAGTGTAAAAGAGCCATA+AGG + chr3_4:66465089-66465108 None:intergenic 40.0%
TGTGTGTGACTGAGAAGGAA+TGG + chr3_4:66463658-66463677 None:intergenic 45.0%
AGTTAGAGAGAGAGAGAGAG+GGG + chr3_4:66463733-66463752 None:intergenic 45.0%
CTAGTTAGAGAGAGAGAGAG+AGG + chr3_4:66463735-66463754 None:intergenic 45.0%
! CTTCTCATAATTGGTGGTCG+AGG + chr3_4:66463870-66463889 None:intergenic 45.0%
! AGAACAAGAAGCATGAGTGG+TGG - chr3_4:66463888-66463907 Msa0515280:three_prime_UTR 45.0%
!! AGGTTGTTGGTTTGATCAGG+TGG + chr3_4:66463933-66463952 None:intergenic 45.0%
CAACCTAGAATCCCATCATG+AGG - chr3_4:66463947-66463966 Msa0515280:three_prime_UTR 45.0%
! CTTCCTCATGATGGGATTCT+AGG + chr3_4:66463953-66463972 None:intergenic 45.0%
TGATCATCATGGTGTGGATG+TGG + chr3_4:66464008-66464027 None:intergenic 45.0%
GAATCCACATACGGTTGTGA+TGG - chr3_4:66464049-66464068 Msa0515280:CDS 45.0%
GTGGCTGATGAACGTTTAGT+GGG - chr3_4:66464104-66464123 Msa0515280:CDS 45.0%
TCATGTGCACTCACACTCAA+AGG + chr3_4:66464203-66464222 None:intergenic 45.0%
CCACAAGTTTGGAGAAAGTG+AGG + chr3_4:66464242-66464261 None:intergenic 45.0%
CCTCACTTTCTCCAAACTTG+TGG - chr3_4:66464239-66464258 Msa0515280:intron 45.0%
AGTTACAGAGGCCACAAGTT+TGG + chr3_4:66464253-66464272 None:intergenic 45.0%
TACATGTAGATGTCTCGACC+AGG + chr3_4:66464287-66464306 None:intergenic 45.0%
TGTAAACCCTACTCTGCCTA+GGG - chr3_4:66464355-66464374 Msa0515280:intron 45.0%
GTAATCCGGAGTTCAGTCAA+AGG - chr3_4:66464393-66464412 Msa0515280:intron 45.0%
!! CATTTTGCAGTTTGGAGTGC+AGG - chr3_4:66464760-66464779 Msa0515280:CDS 45.0%
TACACGGTGGTCTTCTTTGT+TGG + chr3_4:66464838-66464857 None:intergenic 45.0%
ACAAGCAGCAGTAGAAGCTT+TGG + chr3_4:66464883-66464902 None:intergenic 45.0%
! CTGCTGCTTGTACTTGAAGA+AGG - chr3_4:66464892-66464911 Msa0515280:CDS 45.0%
TTGAGACAAAGGGAGAAACG+AGG + chr3_4:66465056-66465075 None:intergenic 45.0%
TGTGTGTGACTGAGAAGGAA+TGG + chr3_4:66463658-66463677 None:intergenic 45.0%
AGTTAGAGAGAGAGAGAGAG+GGG + chr3_4:66463733-66463752 None:intergenic 45.0%
CTAGTTAGAGAGAGAGAGAG+AGG + chr3_4:66463735-66463754 None:intergenic 45.0%
! CTTCTCATAATTGGTGGTCG+AGG + chr3_4:66463870-66463889 None:intergenic 45.0%
! AGAACAAGAAGCATGAGTGG+TGG - chr3_4:66463888-66463907 Msa0515280:three_prime_UTR 45.0%
!! AGGTTGTTGGTTTGATCAGG+TGG + chr3_4:66463933-66463952 None:intergenic 45.0%
CAACCTAGAATCCCATCATG+AGG - chr3_4:66463947-66463966 Msa0515280:three_prime_UTR 45.0%
! CTTCCTCATGATGGGATTCT+AGG + chr3_4:66463953-66463972 None:intergenic 45.0%
TGATCATCATGGTGTGGATG+TGG + chr3_4:66464008-66464027 None:intergenic 45.0%
GAATCCACATACGGTTGTGA+TGG - chr3_4:66464049-66464068 Msa0515280:CDS 45.0%
GTGGCTGATGAACGTTTAGT+GGG - chr3_4:66464104-66464123 Msa0515280:CDS 45.0%
TCATGTGCACTCACACTCAA+AGG + chr3_4:66464203-66464222 None:intergenic 45.0%
CCACAAGTTTGGAGAAAGTG+AGG + chr3_4:66464242-66464261 None:intergenic 45.0%
CCTCACTTTCTCCAAACTTG+TGG - chr3_4:66464239-66464258 Msa0515280:intron 45.0%
AGTTACAGAGGCCACAAGTT+TGG + chr3_4:66464253-66464272 None:intergenic 45.0%
TACATGTAGATGTCTCGACC+AGG + chr3_4:66464287-66464306 None:intergenic 45.0%
TGTAAACCCTACTCTGCCTA+GGG - chr3_4:66464355-66464374 Msa0515280:intron 45.0%
GTAATCCGGAGTTCAGTCAA+AGG - chr3_4:66464393-66464412 Msa0515280:intron 45.0%
!! CATTTTGCAGTTTGGAGTGC+AGG - chr3_4:66464760-66464779 Msa0515280:CDS 45.0%
TACACGGTGGTCTTCTTTGT+TGG + chr3_4:66464838-66464857 None:intergenic 45.0%
ACAAGCAGCAGTAGAAGCTT+TGG + chr3_4:66464883-66464902 None:intergenic 45.0%
! CTGCTGCTTGTACTTGAAGA+AGG - chr3_4:66464892-66464911 Msa0515280:CDS 45.0%
TTGAGACAAAGGGAGAAACG+AGG + chr3_4:66465056-66465075 None:intergenic 45.0%
GTTAGAGAGAGAGAGAGAGG+GGG + chr3_4:66463732-66463751 None:intergenic 50.0%
ATCAGGTGGTTGCATGTCCA+CGG + chr3_4:66463919-66463938 None:intergenic 50.0%
GTTCCCATCACAACCGTATG+TGG + chr3_4:66464056-66464075 None:intergenic 50.0%
GGTGGCTGATGAACGTTTAG+TGG - chr3_4:66464103-66464122 Msa0515280:CDS 50.0%
TGGCCTCTGTAACTGTCGTT+TGG - chr3_4:66464259-66464278 Msa0515280:intron 50.0%
GAGCCAAACGACAGTTACAG+AGG + chr3_4:66464265-66464284 None:intergenic 50.0%
! TGTAACTGTCGTTTGGCTCC+TGG - chr3_4:66464266-66464285 Msa0515280:intron 50.0%
GTGTAAACCCTACTCTGCCT+AGG - chr3_4:66464354-66464373 Msa0515280:intron 50.0%
AAGGCAGAGCCTTCGTAATC+CGG - chr3_4:66464379-66464398 Msa0515280:intron 50.0%
GACTGAACTCCGGATTACGA+AGG + chr3_4:66464391-66464410 None:intergenic 50.0%
! AGCAGTAGAAGCTTTGGCAG+TGG + chr3_4:66464877-66464896 None:intergenic 50.0%
AGACAAAGGGAGAAACGAGG+TGG + chr3_4:66465053-66465072 None:intergenic 50.0%
GTTAGAGAGAGAGAGAGAGG+GGG + chr3_4:66463732-66463751 None:intergenic 50.0%
ATCAGGTGGTTGCATGTCCA+CGG + chr3_4:66463919-66463938 None:intergenic 50.0%
GTTCCCATCACAACCGTATG+TGG + chr3_4:66464056-66464075 None:intergenic 50.0%
GGTGGCTGATGAACGTTTAG+TGG - chr3_4:66464103-66464122 Msa0515280:CDS 50.0%
TGGCCTCTGTAACTGTCGTT+TGG - chr3_4:66464259-66464278 Msa0515280:intron 50.0%
GAGCCAAACGACAGTTACAG+AGG + chr3_4:66464265-66464284 None:intergenic 50.0%
! TGTAACTGTCGTTTGGCTCC+TGG - chr3_4:66464266-66464285 Msa0515280:intron 50.0%
GTGTAAACCCTACTCTGCCT+AGG - chr3_4:66464354-66464373 Msa0515280:intron 50.0%
AAGGCAGAGCCTTCGTAATC+CGG - chr3_4:66464379-66464398 Msa0515280:intron 50.0%
GACTGAACTCCGGATTACGA+AGG + chr3_4:66464391-66464410 None:intergenic 50.0%
! AGCAGTAGAAGCTTTGGCAG+TGG + chr3_4:66464877-66464896 None:intergenic 50.0%
AGACAAAGGGAGAAACGAGG+TGG + chr3_4:66465053-66465072 None:intergenic 50.0%
! AGGGGTGTGTGTGACTGAGA+AGG + chr3_4:66463663-66463682 None:intergenic 55.0%
AGAGAGAGGGGGAGAGAGAT+GGG + chr3_4:66463721-66463740 None:intergenic 55.0%
! CATGAGTGGTGGTTTGTCCG+TGG - chr3_4:66463899-66463918 Msa0515280:three_prime_UTR 55.0%
TGGCTGTGGCTGTGTGTGAA+GGG + chr3_4:66464145-66464164 None:intergenic 55.0%
! AGGGGTGTGTGTGACTGAGA+AGG + chr3_4:66463663-66463682 None:intergenic 55.0%
AGAGAGAGGGGGAGAGAGAT+GGG + chr3_4:66463721-66463740 None:intergenic 55.0%
! CATGAGTGGTGGTTTGTCCG+TGG - chr3_4:66463899-66463918 Msa0515280:three_prime_UTR 55.0%
TGGCTGTGGCTGTGTGTGAA+GGG + chr3_4:66464145-66464164 None:intergenic 55.0%
GAGAGAGAGGGGGAGAGAGA+TGG + chr3_4:66463722-66463741 None:intergenic 60.0%
TGGTGTGGATGTGGCTGCAG+AGG + chr3_4:66463999-66464018 None:intergenic 60.0%
GTGGCTGTGGCTGTGTGTGA+AGG + chr3_4:66464146-66464165 None:intergenic 60.0%
ACCCTACTCTGCCTAGGGCA+AGG - chr3_4:66464360-66464379 Msa0515280:intron 60.0%
GCCTTGCCCTAGGCAGAGTA+GGG + chr3_4:66464364-66464383 None:intergenic 60.0%
TGCCTTGCCCTAGGCAGAGT+AGG + chr3_4:66464365-66464384 None:intergenic 60.0%
TGCACGGTGGTGATACACGG+TGG + chr3_4:66464851-66464870 None:intergenic 60.0%
CCGTGCACGGTGGTGATACA+CGG + chr3_4:66464854-66464873 None:intergenic 60.0%
CCGTGTATCACCACCGTGCA+CGG - chr3_4:66464851-66464870 Msa0515280:CDS 60.0%
GAGAGAGAGGGGGAGAGAGA+TGG + chr3_4:66463722-66463741 None:intergenic 60.0%
TGGTGTGGATGTGGCTGCAG+AGG + chr3_4:66463999-66464018 None:intergenic 60.0%
GTGGCTGTGGCTGTGTGTGA+AGG + chr3_4:66464146-66464165 None:intergenic 60.0%
ACCCTACTCTGCCTAGGGCA+AGG - chr3_4:66464360-66464379 Msa0515280:intron 60.0%
GCCTTGCCCTAGGCAGAGTA+GGG + chr3_4:66464364-66464383 None:intergenic 60.0%
TGCCTTGCCCTAGGCAGAGT+AGG + chr3_4:66464365-66464384 None:intergenic 60.0%
TGCACGGTGGTGATACACGG+TGG + chr3_4:66464851-66464870 None:intergenic 60.0%
CCGTGCACGGTGGTGATACA+CGG + chr3_4:66464854-66464873 None:intergenic 60.0%
CCGTGTATCACCACCGTGCA+CGG - chr3_4:66464851-66464870 Msa0515280:CDS 60.0%
GAGAGGGGGAGAGAGATGGG+TGG + chr3_4:66463718-66463737 None:intergenic 65.0%
CGAAGGCTCTGCCTTGCCCT+AGG + chr3_4:66464374-66464393 None:intergenic 65.0%
GCTTTGGCAGTGGCCGTGCA+CGG + chr3_4:66464867-66464886 None:intergenic 65.0%
GAGAGGGGGAGAGAGATGGG+TGG + chr3_4:66463718-66463737 None:intergenic 65.0%
CGAAGGCTCTGCCTTGCCCT+AGG + chr3_4:66464374-66464393 None:intergenic 65.0%
GCTTTGGCAGTGGCCGTGCA+CGG + chr3_4:66464867-66464886 None:intergenic 65.0%
TTGGCAGTGGCCGTGCACGG+TGG + chr3_4:66464864-66464883 None:intergenic 70.0%
TTGGCAGTGGCCGTGCACGG+TGG + chr3_4:66464864-66464883 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 66463653 66465269 66463653 ID=Msa0515280;Name=Msa0515280
chr3_4 mRNA 66463653 66465269 66463653 ID=Msa0515280-mRNA-1;Parent=Msa0515280;Name=Msa0515280-mRNA-1;_AED=0.01;_eAED=0.01;_QI=196|1|1|1|1|1|2|361|206
chr3_4 exon 66463653 66464173 66463653 ID=Msa0515280-mRNA-1:exon:17098;Parent=Msa0515280-mRNA-1
chr3_4 exon 66464613 66465269 66464613 ID=Msa0515280-mRNA-1:exon:17097;Parent=Msa0515280-mRNA-1
chr3_4 five_prime_UTR 66465074 66465269 66465074 ID=Msa0515280-mRNA-1:five_prime_utr;Parent=Msa0515280-mRNA-1
chr3_4 CDS 66464613 66465073 66464613 ID=Msa0515280-mRNA-1:cds;Parent=Msa0515280-mRNA-1
chr3_4 CDS 66464014 66464173 66464014 ID=Msa0515280-mRNA-1:cds;Parent=Msa0515280-mRNA-1
chr3_4 three_prime_UTR 66463653 66464013 66463653 ID=Msa0515280-mRNA-1:three_prime_utr;Parent=Msa0515280-mRNA-1
Gene Sequence

>Msa0515280

ATGTTGCTTGGAAAACGTCCTCGACCACCAATTATGAGAAGAACAAGAAGCATGAGTGGTGGTTTGTCCGTGGACATGCAACCACCTGATCAAACCAACAACCTAGAATCCCATCATGAGGAAGAATCAGTGATGTCTCATCAGAATCCTCTGCAGCCACATCCACACCATGATGATCAAGAACATGGCATCAACATCAAGAATCCACATACGGTTGTGATGGGAACAGAAACACACATTAAAAGTGAATTAACGGTGGCTGATGAACGTTTAGTGGGTAGTGCAGTCATGTTCCCTTCACACACAGCCACAGCCACAAACACTAACAATATTATTAATCATAATAATAATCCTTTGAGTGTGAGTGCACATGATGTCATACATAGTACACCTCACTTTCTCCAAACTTGTGGCCTCTGTAACTGTCGTTTGGCTCCTGGTCGAGACATCTACATGTATAGGGGAGATACAGCATTTTGCAGTTTGGAGTGCAGGGAGCAACAAATAAAACAAGACAAAAGAAAAGAAAAGTGGAAGATGGCGTTCACCAACAAAGAAGACCACCGTGTATCACCACCGTGCACGGCCACTGCCAAAGCTTCTACTGCTGCTTGTACTTGA
Protein sequence

>Msa0515280

MLLGKRPRPPIMRRTRSMSGGLSVDMQPPDQTNNLESHHEEESVMSHQNPLQPHPHHDDQEHGINIKNPHTVVMGTETHIKSELTVADERLVGSAVMFPSHTATATNTNNIINHNNNPLSVSAHDVIHSTPHFLQTCGLCNCRLAPGRDIYMYRGDTAFCSLECREQQIKQDKRKEKWKMAFTNKEDHRVSPPCTATAKASTAACT*