AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0436930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0436930 MtrunA17_Chr3g0124361 89.175 194 10 3 1 193 1 184 3.93e-125 351
Msa0436930 MtrunA17_Chr3g0115551 33.898 177 74 7 1 177 1 134 2.05e-21 86.3
Msa0436930 MtrunA17_Chr8g0336591 53.571 56 25 1 127 181 32 87 2.14e-15 69.3
Msa0436930 MtrunA17_Chr4g0055191 61.702 47 18 0 131 177 71 117 5.80e-15 69.3
Msa0436930 MtrunA17_Chr5g0400051 50.704 71 29 2 131 196 83 152 1.38e-14 68.6
Msa0436930 MtrunA17_Chr8g0344991 44.286 70 36 1 133 202 39 105 6.96e-14 65.5
Msa0436930 MtrunA17_Chr4g0070221 54.902 51 22 1 127 177 172 221 9.15e-12 62.4
Msa0436930 MtrunA17_Chr2g0295651 58.537 41 17 0 133 173 64 104 9.79e-12 60.5
Msa0436930 MtrunA17_Chr2g0301141 40.741 81 45 2 101 179 152 231 1.57e-11 61.6
Msa0436930 MtrunA17_Chr4g0067541 43.548 62 34 1 114 175 44 104 4.42e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0436930 AT1G78020.1 33.010 206 94 7 1 206 1 162 1.57e-23 92.4
Msa0436930 AT1G22160.1 35.196 179 61 8 1 179 1 124 2.20e-19 81.3
Msa0436930 AT4G39795.1 45.833 72 36 1 112 183 55 123 6.08e-17 74.3
Msa0436930 AT4G17670.1 62.000 50 19 0 128 177 71 120 1.35e-16 73.9
Msa0436930 AT5G65040.1 51.389 72 31 1 114 185 42 109 1.63e-16 72.8
Msa0436930 AT2G44670.1 62.000 50 18 1 131 179 14 63 6.14e-16 70.5
Msa0436930 AT5G47060.2 63.830 47 17 0 131 177 95 141 5.87e-15 70.1
Msa0436930 AT5G47060.1 63.830 47 17 0 131 177 95 141 5.87e-15 70.1
Msa0436930 AT1G53885.1 48.529 68 33 1 109 176 20 85 1.78e-12 62.4
Msa0436930 AT1G53903.1 48.529 68 33 1 109 176 20 85 1.78e-12 62.4
Msa0436930 AT1G19200.1 49.091 55 27 1 126 180 143 196 4.97e-11 60.1
Msa0436930 AT1G74940.1 45.902 61 32 1 132 192 149 208 5.45e-11 60.1
Msa0436930 AT5G49120.1 39.130 69 42 0 105 173 40 108 5.83e-11 58.9
Msa0436930 AT1G19200.2 49.091 55 27 1 126 180 156 209 6.13e-11 60.1

Find 67 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGTTCTTCTCATAAT+TGG 0.156033 3_2:+90821790 None:intergenic
CTCAATTATTATGCTCCTTA+TGG 0.202337 3_2:-90820593 Msa0436930:three_prime_UTR
CTTCCTCATGATGGGATTCT+AGG 0.211376 3_2:+90821716 None:intergenic
ACACTTGAATAATTATTATA+AGG 0.265097 3_2:-90820744 Msa0436930:three_prime_UTR
AATAATATTGTTAGTGTTTG+TGG 0.326456 3_2:+90821504 None:intergenic
AGTTACAGAGGCCACAAGTT+TGG 0.349033 3_2:+90821416 None:intergenic
ATGATGGGATTCTAGGTTGT+TGG 0.366587 3_2:+90821723 None:intergenic
ACAAGCAGCAGTAGAAGCTT+TGG 0.372677 3_2:+90820784 None:intergenic
TCTAGGTTGTTGGTTTGATC+AGG 0.381479 3_2:+90821733 None:intergenic
TACTAGAAACTTTGTAAAGA+AGG 0.385710 3_2:-90820523 Msa0436930:three_prime_UTR
ATTGTTAGTGTTTGTGGCTG+TGG 0.394454 3_2:+90821510 None:intergenic
TGTAACTGTCGTTTGGCTCC+TGG 0.404985 3_2:-90821400 Msa0436930:CDS
TACACGGTGGTCTTCTTTGT+TGG 0.433148 3_2:+90820829 None:intergenic
ACACATTAAAAGTGAATTAA+CGG 0.437387 3_2:-90821584 Msa0436930:CDS
CTGCTGCTTGTACTTGAAGA+AGG 0.439916 3_2:-90820772 Msa0436930:exon
TGGCCTCTGTAACTGTCGTT+TGG 0.455985 3_2:-90821407 Msa0436930:CDS
CTTGTTCTTCTCATAATTGG+TGG 0.464977 3_2:+90821793 None:intergenic
CACTGATTCTTCCTCATGAT+GGG 0.470672 3_2:+90821708 None:intergenic
AGCATAATAATTGAGACAAA+GGG 0.486017 3_2:+90820601 None:intergenic
GATTAGAAGAACATGTTGCT+TGG 0.489313 3_2:-90821829 Msa0436930:exon
TCACTGATTCTTCCTCATGA+TGG 0.491322 3_2:+90821707 None:intergenic
AAGACAAAAGAAAAGAAAAG+TGG 0.499141 3_2:-90820865 Msa0436930:CDS
GTCTTGATGTTAGAAATAGT+TGG 0.501218 3_2:+90821868 None:intergenic
GTTCCCATCACAACCGTATG+TGG 0.501901 3_2:+90821613 None:intergenic
GAATCCACATACGGTTGTGA+TGG 0.509624 3_2:-90821617 Msa0436930:CDS
TGATCATCATGGTGTGGATG+TGG 0.510086 3_2:+90821661 None:intergenic
GTGGCTGTGGCTGTGTGTGA+AGG 0.511685 3_2:+90821523 None:intergenic
GAGCATAATAATTGAGACAA+AGG 0.520661 3_2:+90820600 None:intergenic
GGTGGCTGATGAACGTTTAG+TGG 0.522207 3_2:-90821563 Msa0436930:CDS
GTCGAGACATCTACATGTAT+AGG 0.522956 3_2:-90821378 Msa0436930:intron
ATCAGGTGGTTGCATGTCCA+CGG 0.524115 3_2:+90821750 None:intergenic
AAGAAAAGAAAAGTGGAAGA+TGG 0.529987 3_2:-90820858 Msa0436930:CDS
TTGTGCACTTACGACAAAGA+AGG 0.537138 3_2:-90820638 Msa0436930:three_prime_UTR
TGTGTGTGACTGAGAAGGAA+TGG 0.540588 3_2:+90822017 None:intergenic
CTTCTCATAATTGGTGGTCG+AGG 0.540724 3_2:+90821799 None:intergenic
AGGTTGTTGGTTTGATCAGG+TGG 0.542107 3_2:+90821736 None:intergenic
TGGCTGTGGCTGTGTGTGAA+GGG 0.543537 3_2:+90821524 None:intergenic
TGCCATGTTCTTGATCATCA+TGG 0.545211 3_2:+90821650 None:intergenic
AGCAGTAGAAGCTTTGGCAG+TGG 0.548192 3_2:+90820790 None:intergenic
ACCAGTGTAAAAGAGCCATA+AGG 0.561274 3_2:+90820578 None:intergenic
GCTTTGGCAGTGGCCGTGCA+CGG 0.562038 3_2:+90820800 None:intergenic
TCATGTGCACTCACACTCAA+AGG 0.576185 3_2:+90821466 None:intergenic
CCGTGTATCACCACCGTGCA+CGG 0.577796 3_2:-90820813 Msa0436930:CDS
AATCCACATACGGTTGTGAT+GGG 0.578470 3_2:-90821616 Msa0436930:CDS
CCTCACTTTCTCCAAACTTG+TGG 0.579241 3_2:-90821427 Msa0436930:CDS
AGGGGTGTGTGTGACTGAGA+AGG 0.581702 3_2:+90822012 None:intergenic
TGCTGCTTGTACTTGAAGAA+GGG 0.586632 3_2:-90820771 Msa0436930:exon
CCGTGCACGGTGGTGATACA+CGG 0.602872 3_2:+90820813 None:intergenic
CACCATGATGATCAAGAACA+TGG 0.605152 3_2:-90821652 Msa0436930:CDS
TGGTGTGGATGTGGCTGCAG+AGG 0.618105 3_2:+90821670 None:intergenic
GTGGCTGATGAACGTTTAGT+GGG 0.621978 3_2:-90821562 Msa0436930:CDS
CCACAAGTTTGGAGAAAGTG+AGG 0.629285 3_2:+90821427 None:intergenic
TGTTCTTGATCATCATGGTG+TGG 0.629577 3_2:+90821655 None:intergenic
CAACATCAAGAATCCACATA+CGG 0.631823 3_2:-90821626 Msa0436930:CDS
CATGAGTGGTGGTTTGTCCG+TGG 0.637137 3_2:-90821767 Msa0436930:CDS
CATGAATGACATTGTAAGCG+TGG 0.637323 3_2:+90820671 None:intergenic
AGAAGAACAAGAAGCATGAG+TGG 0.643370 3_2:-90821781 Msa0436930:CDS
TACATGTAGATGTCTCGACC+AGG 0.645212 3_2:+90821382 None:intergenic
CAACCTAGAATCCCATCATG+AGG 0.662994 3_2:-90821719 Msa0436930:CDS
TTGGCAGTGGCCGTGCACGG+TGG 0.682224 3_2:+90820803 None:intergenic
GAGCCAAACGACAGTTACAG+AGG 0.685876 3_2:+90821404 None:intergenic
AGAACAAGAAGCATGAGTGG+TGG 0.691730 3_2:-90821778 Msa0436930:CDS
CATTAAAAGTGAATTAACGG+TGG 0.697236 3_2:-90821581 Msa0436930:CDS
AGACAAAGGGAGAAACGAGG+TGG 0.714534 3_2:+90820614 None:intergenic
GTGTGACTGAGAAGGAATGG+TGG 0.728901 3_2:+90822020 None:intergenic
TGCACGGTGGTGATACACGG+TGG 0.754526 3_2:+90820816 None:intergenic
TTGAGACAAAGGGAGAAACG+AGG 0.774479 3_2:+90820611 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGAATAATAGAAAAAAA+TGG + chr3_2:90821091-90821110 None:intergenic 10.0%
!!! TTAATTAATTTTTTGTTTAG+AGG - chr3_2:90821425-90821444 Msa0436930:CDS 10.0%
!! TAATGAATAATAGAAAAAAA+TGG + chr3_2:90821091-90821110 None:intergenic 10.0%
!!! TTAATTAATTTTTTGTTTAG+AGG - chr3_2:90821425-90821444 Msa0436930:CDS 10.0%
!! GTTAGTTAGTATAATTAATT+TGG - chr3_2:90821456-90821475 Msa0436930:CDS 15.0%
!!! AAAGAAAATTATTGATTTCA+GGG - chr3_2:90821500-90821519 Msa0436930:CDS 15.0%
!! ACACTTGAATAATTATTATA+AGG - chr3_2:90821692-90821711 Msa0436930:CDS 15.0%
!! GTTAGTTAGTATAATTAATT+TGG - chr3_2:90821456-90821475 Msa0436930:CDS 15.0%
!!! AAAGAAAATTATTGATTTCA+GGG - chr3_2:90821500-90821519 Msa0436930:CDS 15.0%
!! ACACTTGAATAATTATTATA+AGG - chr3_2:90821692-90821711 Msa0436930:CDS 15.0%
!! ACACATTAAAAGTGAATTAA+CGG - chr3_2:90820852-90820871 Msa0436930:CDS 20.0%
!! AATAATATTGTTAGTGTTTG+TGG + chr3_2:90820935-90820954 None:intergenic 20.0%
!! GTAAATATTAATTCTTCCTT+TGG + chr3_2:90821319-90821338 None:intergenic 20.0%
!!! GAAAGAAAATTATTGATTTC+AGG - chr3_2:90821499-90821518 Msa0436930:CDS 20.0%
!!! AAGAAAATTATTGATTTCAG+GGG - chr3_2:90821501-90821520 Msa0436930:CDS 20.0%
!! ACACATTAAAAGTGAATTAA+CGG - chr3_2:90820852-90820871 Msa0436930:CDS 20.0%
!! AATAATATTGTTAGTGTTTG+TGG + chr3_2:90820935-90820954 None:intergenic 20.0%
!! GTAAATATTAATTCTTCCTT+TGG + chr3_2:90821319-90821338 None:intergenic 20.0%
!!! GAAAGAAAATTATTGATTTC+AGG - chr3_2:90821499-90821518 Msa0436930:CDS 20.0%
!!! AAGAAAATTATTGATTTCAG+GGG - chr3_2:90821501-90821520 Msa0436930:CDS 20.0%
!! TGACTTTGATATTCTTGTTT+AGG + chr3_2:90821200-90821219 None:intergenic 25.0%
! TAAACAAGAATATCAAAGTC+AGG - chr3_2:90821199-90821218 Msa0436930:intron 25.0%
! CTGAAAATAAAGAGACAATT+AGG + chr3_2:90821289-90821308 None:intergenic 25.0%
!!! TTTTTGTTTAGAGGAAATTG+TGG - chr3_2:90821434-90821453 Msa0436930:CDS 25.0%
! AAGACAAAAGAAAAGAAAAG+TGG - chr3_2:90821571-90821590 Msa0436930:CDS 25.0%
! AGCATAATAATTGAGACAAA+GGG + chr3_2:90821838-90821857 None:intergenic 25.0%
! TACTAGAAACTTTGTAAAGA+AGG - chr3_2:90821913-90821932 Msa0436930:five_prime_UTR 25.0%
!! TGACTTTGATATTCTTGTTT+AGG + chr3_2:90821200-90821219 None:intergenic 25.0%
! TAAACAAGAATATCAAAGTC+AGG - chr3_2:90821199-90821218 Msa0436930:intron 25.0%
! CTGAAAATAAAGAGACAATT+AGG + chr3_2:90821289-90821308 None:intergenic 25.0%
!!! TTTTTGTTTAGAGGAAATTG+TGG - chr3_2:90821434-90821453 Msa0436930:CDS 25.0%
! AAGACAAAAGAAAAGAAAAG+TGG - chr3_2:90821571-90821590 Msa0436930:CDS 25.0%
! AGCATAATAATTGAGACAAA+GGG + chr3_2:90821838-90821857 None:intergenic 25.0%
! TACTAGAAACTTTGTAAAGA+AGG - chr3_2:90821913-90821932 Msa0436930:five_prime_UTR 25.0%
GTCTTGATGTTAGAAATAGT+TGG + chr3_2:90820571-90820590 None:intergenic 30.0%
CTTCTTGTTCTTCTCATAAT+TGG + chr3_2:90820649-90820668 None:intergenic 30.0%
CATTAAAAGTGAATTAACGG+TGG - chr3_2:90820855-90820874 Msa0436930:CDS 30.0%
ACTAACCATAAGAGTTAGTT+AGG - chr3_2:90821226-90821245 Msa0436930:intron 30.0%
TTGAACCTAACTAACTCTTA+TGG + chr3_2:90821234-90821253 None:intergenic 30.0%
!! TATTTTCAGTATCGAACCAA+AGG - chr3_2:90821300-90821319 Msa0436930:intron 30.0%
TTCATTCTAGTAGTTTGACT+AGG - chr3_2:90821348-90821367 Msa0436930:intron 30.0%
AAGAAAAGAAAAGTGGAAGA+TGG - chr3_2:90821578-90821597 Msa0436930:CDS 30.0%
GAGCATAATAATTGAGACAA+AGG + chr3_2:90821839-90821858 None:intergenic 30.0%
CTCAATTATTATGCTCCTTA+TGG - chr3_2:90821843-90821862 Msa0436930:five_prime_UTR 30.0%
!! ACTAGCTATTTGAGTTTTAC+TGG - chr3_2:90821943-90821962 Msa0436930:five_prime_UTR 30.0%
GTCTTGATGTTAGAAATAGT+TGG + chr3_2:90820571-90820590 None:intergenic 30.0%
CTTCTTGTTCTTCTCATAAT+TGG + chr3_2:90820649-90820668 None:intergenic 30.0%
CATTAAAAGTGAATTAACGG+TGG - chr3_2:90820855-90820874 Msa0436930:CDS 30.0%
ACTAACCATAAGAGTTAGTT+AGG - chr3_2:90821226-90821245 Msa0436930:intron 30.0%
TTGAACCTAACTAACTCTTA+TGG + chr3_2:90821234-90821253 None:intergenic 30.0%
!! TATTTTCAGTATCGAACCAA+AGG - chr3_2:90821300-90821319 Msa0436930:intron 30.0%
TTCATTCTAGTAGTTTGACT+AGG - chr3_2:90821348-90821367 Msa0436930:intron 30.0%
AAGAAAAGAAAAGTGGAAGA+TGG - chr3_2:90821578-90821597 Msa0436930:CDS 30.0%
GAGCATAATAATTGAGACAA+AGG + chr3_2:90821839-90821858 None:intergenic 30.0%
CTCAATTATTATGCTCCTTA+TGG - chr3_2:90821843-90821862 Msa0436930:five_prime_UTR 30.0%
!! ACTAGCTATTTGAGTTTTAC+TGG - chr3_2:90821943-90821962 Msa0436930:five_prime_UTR 30.0%
AGTTCACACAAAAACAGAGA+GGG + chr3_2:90820446-90820465 None:intergenic 35.0%
GATTAGAAGAACATGTTGCT+TGG - chr3_2:90820607-90820626 Msa0436930:three_prime_UTR 35.0%
CTTGTTCTTCTCATAATTGG+TGG + chr3_2:90820646-90820665 None:intergenic 35.0%
CAACATCAAGAATCCACATA+CGG - chr3_2:90820810-90820829 Msa0436930:CDS 35.0%
AGACATCTACATGTATAGGT+AGG - chr3_2:90821062-90821081 Msa0436930:intron 35.0%
! TTTTACCTTTGACTGAACTC+CGG + chr3_2:90821173-90821192 None:intergenic 35.0%
! AGATACAGCATTTTGCAGTT+TGG - chr3_2:90821524-90821543 Msa0436930:CDS 35.0%
AGTTCACACAAAAACAGAGA+GGG + chr3_2:90820446-90820465 None:intergenic 35.0%
GATTAGAAGAACATGTTGCT+TGG - chr3_2:90820607-90820626 Msa0436930:three_prime_UTR 35.0%
CTTGTTCTTCTCATAATTGG+TGG + chr3_2:90820646-90820665 None:intergenic 35.0%
CAACATCAAGAATCCACATA+CGG - chr3_2:90820810-90820829 Msa0436930:CDS 35.0%
AGACATCTACATGTATAGGT+AGG - chr3_2:90821062-90821081 Msa0436930:intron 35.0%
! TTTTACCTTTGACTGAACTC+CGG + chr3_2:90821173-90821192 None:intergenic 35.0%
! AGATACAGCATTTTGCAGTT+TGG - chr3_2:90821524-90821543 Msa0436930:CDS 35.0%
GTTCACACAAAAACAGAGAG+GGG + chr3_2:90820445-90820464 None:intergenic 40.0%
GAGTTCACACAAAAACAGAG+AGG + chr3_2:90820447-90820466 None:intergenic 40.0%
! AGAAGAACAAGAAGCATGAG+TGG - chr3_2:90820655-90820674 Msa0436930:three_prime_UTR 40.0%
!! TCTAGGTTGTTGGTTTGATC+AGG + chr3_2:90820706-90820725 None:intergenic 40.0%
! ATGATGGGATTCTAGGTTGT+TGG + chr3_2:90820716-90820735 None:intergenic 40.0%
!! CACTGATTCTTCCTCATGAT+GGG + chr3_2:90820731-90820750 None:intergenic 40.0%
!! TCACTGATTCTTCCTCATGA+TGG + chr3_2:90820732-90820751 None:intergenic 40.0%
TGTTCTTGATCATCATGGTG+TGG + chr3_2:90820784-90820803 None:intergenic 40.0%
CACCATGATGATCAAGAACA+TGG - chr3_2:90820784-90820803 Msa0436930:CDS 40.0%
TGCCATGTTCTTGATCATCA+TGG + chr3_2:90820789-90820808 None:intergenic 40.0%
AATCCACATACGGTTGTGAT+GGG - chr3_2:90820820-90820839 Msa0436930:CDS 40.0%
ATTGTTAGTGTTTGTGGCTG+TGG + chr3_2:90820929-90820948 None:intergenic 40.0%
GTCGAGACATCTACATGTAT+AGG - chr3_2:90821058-90821077 Msa0436930:intron 40.0%
!! ATTTTGCAGTTTGGAGTGCA+GGG - chr3_2:90821533-90821552 Msa0436930:CDS 40.0%
! TGCTGCTTGTACTTGAAGAA+GGG - chr3_2:90821665-90821684 Msa0436930:CDS 40.0%
CATGAATGACATTGTAAGCG+TGG + chr3_2:90821768-90821787 None:intergenic 40.0%
TTGTGCACTTACGACAAAGA+AGG - chr3_2:90821798-90821817 Msa0436930:CDS 40.0%
! TCCTTATGGCTCTTTTACAC+TGG - chr3_2:90821857-90821876 Msa0436930:five_prime_UTR 40.0%
ACCAGTGTAAAAGAGCCATA+AGG + chr3_2:90821861-90821880 None:intergenic 40.0%
GTTCACACAAAAACAGAGAG+GGG + chr3_2:90820445-90820464 None:intergenic 40.0%
GAGTTCACACAAAAACAGAG+AGG + chr3_2:90820447-90820466 None:intergenic 40.0%
! AGAAGAACAAGAAGCATGAG+TGG - chr3_2:90820655-90820674 Msa0436930:three_prime_UTR 40.0%
!! TCTAGGTTGTTGGTTTGATC+AGG + chr3_2:90820706-90820725 None:intergenic 40.0%
! ATGATGGGATTCTAGGTTGT+TGG + chr3_2:90820716-90820735 None:intergenic 40.0%
!! CACTGATTCTTCCTCATGAT+GGG + chr3_2:90820731-90820750 None:intergenic 40.0%
!! TCACTGATTCTTCCTCATGA+TGG + chr3_2:90820732-90820751 None:intergenic 40.0%
TGTTCTTGATCATCATGGTG+TGG + chr3_2:90820784-90820803 None:intergenic 40.0%
CACCATGATGATCAAGAACA+TGG - chr3_2:90820784-90820803 Msa0436930:CDS 40.0%
TGCCATGTTCTTGATCATCA+TGG + chr3_2:90820789-90820808 None:intergenic 40.0%
AATCCACATACGGTTGTGAT+GGG - chr3_2:90820820-90820839 Msa0436930:CDS 40.0%
ATTGTTAGTGTTTGTGGCTG+TGG + chr3_2:90820929-90820948 None:intergenic 40.0%
GTCGAGACATCTACATGTAT+AGG - chr3_2:90821058-90821077 Msa0436930:intron 40.0%
!! ATTTTGCAGTTTGGAGTGCA+GGG - chr3_2:90821533-90821552 Msa0436930:CDS 40.0%
! TGCTGCTTGTACTTGAAGAA+GGG - chr3_2:90821665-90821684 Msa0436930:CDS 40.0%
CATGAATGACATTGTAAGCG+TGG + chr3_2:90821768-90821787 None:intergenic 40.0%
TTGTGCACTTACGACAAAGA+AGG - chr3_2:90821798-90821817 Msa0436930:CDS 40.0%
! TCCTTATGGCTCTTTTACAC+TGG - chr3_2:90821857-90821876 Msa0436930:five_prime_UTR 40.0%
ACCAGTGTAAAAGAGCCATA+AGG + chr3_2:90821861-90821880 None:intergenic 40.0%
TGTGTGTGACTGAGAAGGAA+TGG + chr3_2:90820422-90820441 None:intergenic 45.0%
TAGAGAGAGAGAGAGAGAGA+GGG + chr3_2:90820500-90820519 None:intergenic 45.0%
TTAGAGAGAGAGAGAGAGAG+AGG + chr3_2:90820501-90820520 None:intergenic 45.0%
! CTTCTCATAATTGGTGGTCG+AGG + chr3_2:90820640-90820659 None:intergenic 45.0%
! AGAACAAGAAGCATGAGTGG+TGG - chr3_2:90820658-90820677 Msa0436930:three_prime_UTR 45.0%
!! AGGTTGTTGGTTTGATCAGG+TGG + chr3_2:90820703-90820722 None:intergenic 45.0%
CAACCTAGAATCCCATCATG+AGG - chr3_2:90820717-90820736 Msa0436930:three_prime_UTR 45.0%
! CTTCCTCATGATGGGATTCT+AGG + chr3_2:90820723-90820742 None:intergenic 45.0%
TGATCATCATGGTGTGGATG+TGG + chr3_2:90820778-90820797 None:intergenic 45.0%
GAATCCACATACGGTTGTGA+TGG - chr3_2:90820819-90820838 Msa0436930:CDS 45.0%
GTGGCTGATGAACGTTTAGT+GGG - chr3_2:90820874-90820893 Msa0436930:CDS 45.0%
TCATGTGCACTCACACTCAA+AGG + chr3_2:90820973-90820992 None:intergenic 45.0%
CCACAAGTTTGGAGAAAGTG+AGG + chr3_2:90821012-90821031 None:intergenic 45.0%
CCTCACTTTCTCCAAACTTG+TGG - chr3_2:90821009-90821028 Msa0436930:intron 45.0%
AGTTACAGAGGCCACAAGTT+TGG + chr3_2:90821023-90821042 None:intergenic 45.0%
TACATGTAGATGTCTCGACC+AGG + chr3_2:90821057-90821076 None:intergenic 45.0%
TGTAAACCCTACTCTGCCTA+GGG - chr3_2:90821127-90821146 Msa0436930:intron 45.0%
GTAATCCGGAGTTCAGTCAA+AGG - chr3_2:90821165-90821184 Msa0436930:intron 45.0%
!! CATTTTGCAGTTTGGAGTGC+AGG - chr3_2:90821532-90821551 Msa0436930:CDS 45.0%
TACACGGTGGTCTTCTTTGT+TGG + chr3_2:90821610-90821629 None:intergenic 45.0%
ACAAGCAGCAGTAGAAGCTT+TGG + chr3_2:90821655-90821674 None:intergenic 45.0%
! CTGCTGCTTGTACTTGAAGA+AGG - chr3_2:90821664-90821683 Msa0436930:CDS 45.0%
TTGAGACAAAGGGAGAAACG+AGG + chr3_2:90821828-90821847 None:intergenic 45.0%
TGTGTGTGACTGAGAAGGAA+TGG + chr3_2:90820422-90820441 None:intergenic 45.0%
TAGAGAGAGAGAGAGAGAGA+GGG + chr3_2:90820500-90820519 None:intergenic 45.0%
TTAGAGAGAGAGAGAGAGAG+AGG + chr3_2:90820501-90820520 None:intergenic 45.0%
! CTTCTCATAATTGGTGGTCG+AGG + chr3_2:90820640-90820659 None:intergenic 45.0%
! AGAACAAGAAGCATGAGTGG+TGG - chr3_2:90820658-90820677 Msa0436930:three_prime_UTR 45.0%
!! AGGTTGTTGGTTTGATCAGG+TGG + chr3_2:90820703-90820722 None:intergenic 45.0%
CAACCTAGAATCCCATCATG+AGG - chr3_2:90820717-90820736 Msa0436930:three_prime_UTR 45.0%
! CTTCCTCATGATGGGATTCT+AGG + chr3_2:90820723-90820742 None:intergenic 45.0%
TGATCATCATGGTGTGGATG+TGG + chr3_2:90820778-90820797 None:intergenic 45.0%
GAATCCACATACGGTTGTGA+TGG - chr3_2:90820819-90820838 Msa0436930:CDS 45.0%
GTGGCTGATGAACGTTTAGT+GGG - chr3_2:90820874-90820893 Msa0436930:CDS 45.0%
TCATGTGCACTCACACTCAA+AGG + chr3_2:90820973-90820992 None:intergenic 45.0%
CCACAAGTTTGGAGAAAGTG+AGG + chr3_2:90821012-90821031 None:intergenic 45.0%
CCTCACTTTCTCCAAACTTG+TGG - chr3_2:90821009-90821028 Msa0436930:intron 45.0%
AGTTACAGAGGCCACAAGTT+TGG + chr3_2:90821023-90821042 None:intergenic 45.0%
TACATGTAGATGTCTCGACC+AGG + chr3_2:90821057-90821076 None:intergenic 45.0%
TGTAAACCCTACTCTGCCTA+GGG - chr3_2:90821127-90821146 Msa0436930:intron 45.0%
GTAATCCGGAGTTCAGTCAA+AGG - chr3_2:90821165-90821184 Msa0436930:intron 45.0%
!! CATTTTGCAGTTTGGAGTGC+AGG - chr3_2:90821532-90821551 Msa0436930:CDS 45.0%
TACACGGTGGTCTTCTTTGT+TGG + chr3_2:90821610-90821629 None:intergenic 45.0%
ACAAGCAGCAGTAGAAGCTT+TGG + chr3_2:90821655-90821674 None:intergenic 45.0%
! CTGCTGCTTGTACTTGAAGA+AGG - chr3_2:90821664-90821683 Msa0436930:CDS 45.0%
TTGAGACAAAGGGAGAAACG+AGG + chr3_2:90821828-90821847 None:intergenic 45.0%
AGAGAGAGAGAGAGAGAGAG+GGG + chr3_2:90820499-90820518 None:intergenic 50.0%
ATCAGGTGGTTGCATGTCCA+CGG + chr3_2:90820689-90820708 None:intergenic 50.0%
GTTCCCATCACAACCGTATG+TGG + chr3_2:90820826-90820845 None:intergenic 50.0%
GGTGGCTGATGAACGTTTAG+TGG - chr3_2:90820873-90820892 Msa0436930:CDS 50.0%
TGGCCTCTGTAACTGTCGTT+TGG - chr3_2:90821029-90821048 Msa0436930:intron 50.0%
GAGCCAAACGACAGTTACAG+AGG + chr3_2:90821035-90821054 None:intergenic 50.0%
! TGTAACTGTCGTTTGGCTCC+TGG - chr3_2:90821036-90821055 Msa0436930:intron 50.0%
GTGTAAACCCTACTCTGCCT+AGG - chr3_2:90821126-90821145 Msa0436930:intron 50.0%
AAGGCAGAGCCTTCGTAATC+CGG - chr3_2:90821151-90821170 Msa0436930:intron 50.0%
GACTGAACTCCGGATTACGA+AGG + chr3_2:90821163-90821182 None:intergenic 50.0%
! AGCAGTAGAAGCTTTGGCAG+TGG + chr3_2:90821649-90821668 None:intergenic 50.0%
AGACAAAGGGAGAAACGAGG+TGG + chr3_2:90821825-90821844 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+GGG + chr3_2:90820499-90820518 None:intergenic 50.0%
ATCAGGTGGTTGCATGTCCA+CGG + chr3_2:90820689-90820708 None:intergenic 50.0%
GTTCCCATCACAACCGTATG+TGG + chr3_2:90820826-90820845 None:intergenic 50.0%
GGTGGCTGATGAACGTTTAG+TGG - chr3_2:90820873-90820892 Msa0436930:CDS 50.0%
TGGCCTCTGTAACTGTCGTT+TGG - chr3_2:90821029-90821048 Msa0436930:intron 50.0%
GAGCCAAACGACAGTTACAG+AGG + chr3_2:90821035-90821054 None:intergenic 50.0%
! TGTAACTGTCGTTTGGCTCC+TGG - chr3_2:90821036-90821055 Msa0436930:intron 50.0%
GTGTAAACCCTACTCTGCCT+AGG - chr3_2:90821126-90821145 Msa0436930:intron 50.0%
AAGGCAGAGCCTTCGTAATC+CGG - chr3_2:90821151-90821170 Msa0436930:intron 50.0%
GACTGAACTCCGGATTACGA+AGG + chr3_2:90821163-90821182 None:intergenic 50.0%
! AGCAGTAGAAGCTTTGGCAG+TGG + chr3_2:90821649-90821668 None:intergenic 50.0%
AGACAAAGGGAGAAACGAGG+TGG + chr3_2:90821825-90821844 None:intergenic 50.0%
! AGGGGTGTGTGTGACTGAGA+AGG + chr3_2:90820427-90820446 None:intergenic 55.0%
AGAGAGAGGGGGAGAGAGAT+GGG + chr3_2:90820487-90820506 None:intergenic 55.0%
GAGAGAGAGAGAGAGAGAGG+GGG + chr3_2:90820498-90820517 None:intergenic 55.0%
! CATGAGTGGTGGTTTGTCCG+TGG - chr3_2:90820669-90820688 Msa0436930:three_prime_UTR 55.0%
TGGCTGTGGCTGTGTGTGAA+GGG + chr3_2:90820915-90820934 None:intergenic 55.0%
! AGGGGTGTGTGTGACTGAGA+AGG + chr3_2:90820427-90820446 None:intergenic 55.0%
AGAGAGAGGGGGAGAGAGAT+GGG + chr3_2:90820487-90820506 None:intergenic 55.0%
GAGAGAGAGAGAGAGAGAGG+GGG + chr3_2:90820498-90820517 None:intergenic 55.0%
! CATGAGTGGTGGTTTGTCCG+TGG - chr3_2:90820669-90820688 Msa0436930:three_prime_UTR 55.0%
TGGCTGTGGCTGTGTGTGAA+GGG + chr3_2:90820915-90820934 None:intergenic 55.0%
GAGAGAGAGGGGGAGAGAGA+TGG + chr3_2:90820488-90820507 None:intergenic 60.0%
TGGTGTGGATGTGGCTGCAG+AGG + chr3_2:90820769-90820788 None:intergenic 60.0%
GTGGCTGTGGCTGTGTGTGA+AGG + chr3_2:90820916-90820935 None:intergenic 60.0%
ACCCTACTCTGCCTAGGGCA+AGG - chr3_2:90821132-90821151 Msa0436930:intron 60.0%
GCCTTGCCCTAGGCAGAGTA+GGG + chr3_2:90821136-90821155 None:intergenic 60.0%
TGCCTTGCCCTAGGCAGAGT+AGG + chr3_2:90821137-90821156 None:intergenic 60.0%
TGCACGGTGGTGATACACGG+TGG + chr3_2:90821623-90821642 None:intergenic 60.0%
CCGTGCACGGTGGTGATACA+CGG + chr3_2:90821626-90821645 None:intergenic 60.0%
CCGTGTATCACCACCGTGCA+CGG - chr3_2:90821623-90821642 Msa0436930:CDS 60.0%
GAGAGAGAGGGGGAGAGAGA+TGG + chr3_2:90820488-90820507 None:intergenic 60.0%
TGGTGTGGATGTGGCTGCAG+AGG + chr3_2:90820769-90820788 None:intergenic 60.0%
GTGGCTGTGGCTGTGTGTGA+AGG + chr3_2:90820916-90820935 None:intergenic 60.0%
ACCCTACTCTGCCTAGGGCA+AGG - chr3_2:90821132-90821151 Msa0436930:intron 60.0%
GCCTTGCCCTAGGCAGAGTA+GGG + chr3_2:90821136-90821155 None:intergenic 60.0%
TGCCTTGCCCTAGGCAGAGT+AGG + chr3_2:90821137-90821156 None:intergenic 60.0%
TGCACGGTGGTGATACACGG+TGG + chr3_2:90821623-90821642 None:intergenic 60.0%
CCGTGCACGGTGGTGATACA+CGG + chr3_2:90821626-90821645 None:intergenic 60.0%
CCGTGTATCACCACCGTGCA+CGG - chr3_2:90821623-90821642 Msa0436930:CDS 60.0%
GAGAGGGGGAGAGAGATGGG+TGG + chr3_2:90820484-90820503 None:intergenic 65.0%
CGAAGGCTCTGCCTTGCCCT+AGG + chr3_2:90821146-90821165 None:intergenic 65.0%
GCTTTGGCAGTGGCCGTGCA+CGG + chr3_2:90821639-90821658 None:intergenic 65.0%
GAGAGGGGGAGAGAGATGGG+TGG + chr3_2:90820484-90820503 None:intergenic 65.0%
CGAAGGCTCTGCCTTGCCCT+AGG + chr3_2:90821146-90821165 None:intergenic 65.0%
GCTTTGGCAGTGGCCGTGCA+CGG + chr3_2:90821639-90821658 None:intergenic 65.0%
TTGGCAGTGGCCGTGCACGG+TGG + chr3_2:90821636-90821655 None:intergenic 70.0%
TTGGCAGTGGCCGTGCACGG+TGG + chr3_2:90821636-90821655 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 90820417 90822041 90820417 ID=Msa0436930;Name=Msa0436930
chr3_2 mRNA 90820417 90822041 90820417 ID=Msa0436930-mRNA-1;Parent=Msa0436930;Name=Msa0436930-mRNA-1;_AED=0.01;_eAED=0.01;_QI=202|1|1|1|1|1|2|361|206
chr3_2 exon 90820417 90820937 90820417 ID=Msa0436930-mRNA-1:exon:27059;Parent=Msa0436930-mRNA-1
chr3_2 exon 90821379 90822041 90821379 ID=Msa0436930-mRNA-1:exon:27058;Parent=Msa0436930-mRNA-1
chr3_2 five_prime_UTR 90821840 90822041 90821840 ID=Msa0436930-mRNA-1:five_prime_utr;Parent=Msa0436930-mRNA-1
chr3_2 CDS 90821379 90821839 90821379 ID=Msa0436930-mRNA-1:cds;Parent=Msa0436930-mRNA-1
chr3_2 CDS 90820778 90820937 90820778 ID=Msa0436930-mRNA-1:cds;Parent=Msa0436930-mRNA-1
chr3_2 three_prime_UTR 90820417 90820777 90820417 ID=Msa0436930-mRNA-1:three_prime_utr;Parent=Msa0436930-mRNA-1
Gene Sequence

>Msa0436930

ATGTTGCTTGGAAAACGTCCTCGACCACCAATTATGAGAAGAACAAGAAGCATGAGTGGTGGTTTGTCCGTGGACATGCAACCACCTGATCAAACCAACAACCTAGAATCCCATCATGAGGAAGAATCAGTGATGTCTCATCAGAATCCTCTGCAGCCACATCCACACCATGATGATCAAGAACATGGCATCAACATCAAGAATCCACATACGGTTGTGATGGGAACAGAAACACACATTAAAAGTGAATTAACGGTGGCTGATGAACGTTTAGTGGGTAGTGCAGTCATGTTCCCTTCACACACAGCCACAGCCACAAACACTAACAATATTATTAATCATAATAATAATCCTTTGAGTGTGAGTGCACATGATGTCATACATAGTACACCTCACTTTCTCCAAACTTGTGGCCTCTGTAACTGTCGTTTGGCTCCTGGTCGAGACATCTACATGTATAGGGGAGATACAGCATTTTGCAGTTTGGAGTGCAGGGAGCAACAAATAAAACAAGACAAAAGAAAAGAAAAGTGGAAGATGGCGTTCACCAACAAAGAAGACCACCGTGTATCACCACCGTGCACGGCCACTGCCAAAGCTTCTACTGCTGCTTGTACTTGA
Protein sequence

>Msa0436930

MLLGKRPRPPIMRRTRSMSGGLSVDMQPPDQTNNLESHHEEESVMSHQNPLQPHPHHDDQEHGINIKNPHTVVMGTETHIKSELTVADERLVGSAVMFPSHTATATNTNNIINHNNNPLSVSAHDVIHSTPHFLQTCGLCNCRLAPGRDIYMYRGDTAFCSLECREQQIKQDKRKEKWKMAFTNKEDHRVSPPCTATAKASTAACT*