AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0469570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469570 MtrunA17_Chr3g0124361 69.565 184 8 2 1 136 1 184 5.41e-83 242
Msa0469570 MtrunA17_Chr3g0115551 34.074 135 73 6 1 120 1 134 9.78e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGTTCTTCTCATAAT+TGG 0.156033 3_3:+71489196 None:intergenic
CTCAATTATTATGCTCCTTA+TGG 0.202337 3_3:-71487998 Msa0469570:three_prime_UTR
CTTCCTCATGATGGGATTCT+AGG 0.211376 3_3:+71489122 None:intergenic
ACACTTGAATAATTATTATA+AGG 0.265097 3_3:-71488149 Msa0469570:three_prime_UTR
ATGATGGGATTCTAGGTTGT+TGG 0.366587 3_3:+71489129 None:intergenic
ACAAGCAGCAGTAGAAGCTT+TGG 0.372677 3_3:+71488189 None:intergenic
TCTAGGTTGTTGGTTTGATC+AGG 0.381479 3_3:+71489139 None:intergenic
TACTAGAAACTTTGTAAAGA+AGG 0.385710 3_3:-71487928 Msa0469570:three_prime_UTR
TACACGGTGGTCTTCTTTGT+TGG 0.433148 3_3:+71488234 None:intergenic
ACACATTAAAAGTGAATTAA+CGG 0.437387 3_3:-71488990 Msa0469570:CDS
CTGCTGCTTGTACTTGAAGA+AGG 0.439916 3_3:-71488177 Msa0469570:exon
CTTGTTCTTCTCATAATTGG+TGG 0.464977 3_3:+71489199 None:intergenic
CACTGATTCTTCCTCATGAT+GGG 0.470672 3_3:+71489114 None:intergenic
AGCATAATAATTGAGACAAA+GGG 0.486017 3_3:+71488006 None:intergenic
GATTAGAAGAACATGTTGCT+TGG 0.489313 3_3:-71489235 None:intergenic
TCACTGATTCTTCCTCATGA+TGG 0.491322 3_3:+71489113 None:intergenic
AAGACAAGAGAAAAGAAAAG+TGG 0.492253 3_3:-71488270 Msa0469570:CDS
GTTCCCATCACAACCGTATG+TGG 0.501901 3_3:+71489019 None:intergenic
GAATCCACATACGGTTGTGA+TGG 0.509624 3_3:-71489023 Msa0469570:CDS
TGATCATCATGGTGTGGATG+TGG 0.510086 3_3:+71489067 None:intergenic
GAGCATAATAATTGAGACAA+AGG 0.520661 3_3:+71488005 None:intergenic
GGTGGCTGATGAACGTTTAG+TGG 0.522207 3_3:-71488969 Msa0469570:CDS
ATCAGGTGGTTGCATGTCCA+CGG 0.524115 3_3:+71489156 None:intergenic
TTGTGCACTTACGACAAAGA+AGG 0.537138 3_3:-71488043 Msa0469570:three_prime_UTR
CTTCTCATAATTGGTGGTCG+AGG 0.540724 3_3:+71489205 None:intergenic
GAGAAAAGAAAAGTGGAAGA+TGG 0.541333 3_3:-71488263 Msa0469570:CDS
AGGTTGTTGGTTTGATCAGG+TGG 0.542107 3_3:+71489142 None:intergenic
TGCCATGTTCTTGATCATCA+TGG 0.545211 3_3:+71489056 None:intergenic
AGCAGTAGAAGCTTTGGCAG+TGG 0.548192 3_3:+71488195 None:intergenic
ACCAGTGTAAAAGAGCCATA+AGG 0.561274 3_3:+71487983 None:intergenic
GCTTTGGCAGTGGCCGTGCA+CGG 0.562038 3_3:+71488205 None:intergenic
CCGTGTATCACCACCGTGCA+CGG 0.577796 3_3:-71488218 Msa0469570:CDS
AATCCACATACGGTTGTGAT+GGG 0.578470 3_3:-71489022 Msa0469570:CDS
TGCTGCTTGTACTTGAAGAA+GGG 0.586632 3_3:-71488176 Msa0469570:exon
CCGTGCACGGTGGTGATACA+CGG 0.602872 3_3:+71488218 None:intergenic
CACCATGATGATCAAGAACA+TGG 0.605152 3_3:-71489058 Msa0469570:CDS
TGGTGTGGATGTGGCTGCAG+AGG 0.618105 3_3:+71489076 None:intergenic
GTGGCTGATGAACGTTTAGT+GGG 0.621978 3_3:-71488968 Msa0469570:CDS
TGTTCTTGATCATCATGGTG+TGG 0.629577 3_3:+71489061 None:intergenic
CAACATCAAGAATCCACATA+CGG 0.631823 3_3:-71489032 Msa0469570:CDS
CATGAGTGGTGGTTTGTCCG+TGG 0.637137 3_3:-71489173 Msa0469570:CDS
CATGAATGACATTGTAAGCG+TGG 0.637323 3_3:+71488076 None:intergenic
AGAAGAACAAGAAGCATGAG+TGG 0.643370 3_3:-71489187 Msa0469570:CDS
CAACCTAGAATCCCATCATG+AGG 0.662994 3_3:-71489125 Msa0469570:CDS
TTGGCAGTGGCCGTGCACGG+TGG 0.682224 3_3:+71488208 None:intergenic
AGAACAAGAAGCATGAGTGG+TGG 0.691730 3_3:-71489184 Msa0469570:CDS
CATTAAAAGTGAATTAACGG+TGG 0.697236 3_3:-71488987 Msa0469570:CDS
AGACAAAGGGAGAAACGAGG+TGG 0.714534 3_3:+71488019 None:intergenic
TGCACGGTGGTGATACACGG+TGG 0.754526 3_3:+71488221 None:intergenic
TTGAGACAAAGGGAGAAACG+AGG 0.774479 3_3:+71488016 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGAATAATAGAAAAAAA+TGG + chr3_3:71488294-71488313 None:intergenic 10.0%
!!! TTAATTAATTTTTTGTTTAG+AGG - chr3_3:71488629-71488648 Msa0469570:intron 10.0%
!! TAATGAATAATAGAAAAAAA+TGG + chr3_3:71488294-71488313 None:intergenic 10.0%
!!! TTAATTAATTTTTTGTTTAG+AGG - chr3_3:71488629-71488648 Msa0469570:intron 10.0%
!! GTTAGTTAGTATAATTAATT+TGG - chr3_3:71488660-71488679 Msa0469570:intron 15.0%
!!! AAAGAAAATTATTGATTTCA+GGG - chr3_3:71488704-71488723 Msa0469570:intron 15.0%
!! ACACTTGAATAATTATTATA+AGG - chr3_3:71488896-71488915 Msa0469570:intron 15.0%
!! GTTAGTTAGTATAATTAATT+TGG - chr3_3:71488660-71488679 Msa0469570:intron 15.0%
!!! AAAGAAAATTATTGATTTCA+GGG - chr3_3:71488704-71488723 Msa0469570:intron 15.0%
!! ACACTTGAATAATTATTATA+AGG - chr3_3:71488896-71488915 Msa0469570:intron 15.0%
!! ACACATTAAAAGTGAATTAA+CGG - chr3_3:71488055-71488074 Msa0469570:three_prime_UTR 20.0%
!! AATAATATTGTTAGTGTTTG+TGG + chr3_3:71488138-71488157 None:intergenic 20.0%
!! GTAAATATTAATTCTTCCTT+TGG + chr3_3:71488523-71488542 None:intergenic 20.0%
!!! GAAAGAAAATTATTGATTTC+AGG - chr3_3:71488703-71488722 Msa0469570:intron 20.0%
!!! AAGAAAATTATTGATTTCAG+GGG - chr3_3:71488705-71488724 Msa0469570:intron 20.0%
!! ACACATTAAAAGTGAATTAA+CGG - chr3_3:71488055-71488074 Msa0469570:three_prime_UTR 20.0%
!! AATAATATTGTTAGTGTTTG+TGG + chr3_3:71488138-71488157 None:intergenic 20.0%
!! GTAAATATTAATTCTTCCTT+TGG + chr3_3:71488523-71488542 None:intergenic 20.0%
!!! GAAAGAAAATTATTGATTTC+AGG - chr3_3:71488703-71488722 Msa0469570:intron 20.0%
!!! AAGAAAATTATTGATTTCAG+GGG - chr3_3:71488705-71488724 Msa0469570:intron 20.0%
!! TGACTTTGATATTCTTGTTT+AGG + chr3_3:71488404-71488423 None:intergenic 25.0%
! TAAACAAGAATATCAAAGTC+AGG - chr3_3:71488403-71488422 Msa0469570:intron 25.0%
! CTGAAAATAAAGAGACAATT+AGG + chr3_3:71488493-71488512 None:intergenic 25.0%
!!! TTTTTGTTTAGAGGAAATTG+TGG - chr3_3:71488638-71488657 Msa0469570:intron 25.0%
! AGCATAATAATTGAGACAAA+GGG + chr3_3:71489042-71489061 None:intergenic 25.0%
! TACTAGAAACTTTGTAAAGA+AGG - chr3_3:71489117-71489136 Msa0469570:CDS 25.0%
!! TGACTTTGATATTCTTGTTT+AGG + chr3_3:71488404-71488423 None:intergenic 25.0%
! TAAACAAGAATATCAAAGTC+AGG - chr3_3:71488403-71488422 Msa0469570:intron 25.0%
! CTGAAAATAAAGAGACAATT+AGG + chr3_3:71488493-71488512 None:intergenic 25.0%
!!! TTTTTGTTTAGAGGAAATTG+TGG - chr3_3:71488638-71488657 Msa0469570:intron 25.0%
! AGCATAATAATTGAGACAAA+GGG + chr3_3:71489042-71489061 None:intergenic 25.0%
! TACTAGAAACTTTGTAAAGA+AGG - chr3_3:71489117-71489136 Msa0469570:CDS 25.0%
CTTCTTGTTCTTCTCATAAT+TGG + chr3_3:71487852-71487871 None:intergenic 30.0%
CATTAAAAGTGAATTAACGG+TGG - chr3_3:71488058-71488077 Msa0469570:three_prime_UTR 30.0%
! TTTTATCTTTGACTGAACTC+CGG + chr3_3:71488377-71488396 None:intergenic 30.0%
TTGAACCTAACTAACTCTTA+TGG + chr3_3:71488438-71488457 None:intergenic 30.0%
!! TATTTTCAGTATCGAACCAA+AGG - chr3_3:71488504-71488523 Msa0469570:intron 30.0%
TTCATTCTAGTAGTTTGACT+AGG - chr3_3:71488552-71488571 Msa0469570:intron 30.0%
AAGACAAGAGAAAAGAAAAG+TGG - chr3_3:71488775-71488794 Msa0469570:intron 30.0%
GAGCATAATAATTGAGACAA+AGG + chr3_3:71489043-71489062 None:intergenic 30.0%
CTCAATTATTATGCTCCTTA+TGG - chr3_3:71489047-71489066 Msa0469570:CDS 30.0%
!! ACTAGCTATTTGAGTTTTAC+TGG - chr3_3:71489147-71489166 Msa0469570:CDS 30.0%
CTTCTTGTTCTTCTCATAAT+TGG + chr3_3:71487852-71487871 None:intergenic 30.0%
CATTAAAAGTGAATTAACGG+TGG - chr3_3:71488058-71488077 Msa0469570:three_prime_UTR 30.0%
! TTTTATCTTTGACTGAACTC+CGG + chr3_3:71488377-71488396 None:intergenic 30.0%
TTGAACCTAACTAACTCTTA+TGG + chr3_3:71488438-71488457 None:intergenic 30.0%
!! TATTTTCAGTATCGAACCAA+AGG - chr3_3:71488504-71488523 Msa0469570:intron 30.0%
TTCATTCTAGTAGTTTGACT+AGG - chr3_3:71488552-71488571 Msa0469570:intron 30.0%
AAGACAAGAGAAAAGAAAAG+TGG - chr3_3:71488775-71488794 Msa0469570:intron 30.0%
GAGCATAATAATTGAGACAA+AGG + chr3_3:71489043-71489062 None:intergenic 30.0%
CTCAATTATTATGCTCCTTA+TGG - chr3_3:71489047-71489066 Msa0469570:CDS 30.0%
!! ACTAGCTATTTGAGTTTTAC+TGG - chr3_3:71489147-71489166 Msa0469570:CDS 30.0%
CTTGTTCTTCTCATAATTGG+TGG + chr3_3:71487849-71487868 None:intergenic 35.0%
CAACATCAAGAATCCACATA+CGG - chr3_3:71488013-71488032 Msa0469570:three_prime_UTR 35.0%
AGACATCTACATGTATAGGT+AGG - chr3_3:71488265-71488284 Msa0469570:CDS 35.0%
ACTAGCCATAAGAGTTAGTT+AGG - chr3_3:71488430-71488449 Msa0469570:intron 35.0%
! AGATACAGCATTTTGCAGTT+TGG - chr3_3:71488728-71488747 Msa0469570:intron 35.0%
GAGAAAAGAAAAGTGGAAGA+TGG - chr3_3:71488782-71488801 Msa0469570:intron 35.0%
CTTGTTCTTCTCATAATTGG+TGG + chr3_3:71487849-71487868 None:intergenic 35.0%
CAACATCAAGAATCCACATA+CGG - chr3_3:71488013-71488032 Msa0469570:three_prime_UTR 35.0%
AGACATCTACATGTATAGGT+AGG - chr3_3:71488265-71488284 Msa0469570:CDS 35.0%
ACTAGCCATAAGAGTTAGTT+AGG - chr3_3:71488430-71488449 Msa0469570:intron 35.0%
! AGATACAGCATTTTGCAGTT+TGG - chr3_3:71488728-71488747 Msa0469570:intron 35.0%
GAGAAAAGAAAAGTGGAAGA+TGG - chr3_3:71488782-71488801 Msa0469570:intron 35.0%
! AGAAGAACAAGAAGCATGAG+TGG - chr3_3:71487858-71487877 Msa0469570:three_prime_UTR 40.0%
!! TCTAGGTTGTTGGTTTGATC+AGG + chr3_3:71487909-71487928 None:intergenic 40.0%
! ATGATGGGATTCTAGGTTGT+TGG + chr3_3:71487919-71487938 None:intergenic 40.0%
!! CACTGATTCTTCCTCATGAT+GGG + chr3_3:71487934-71487953 None:intergenic 40.0%
!! TCACTGATTCTTCCTCATGA+TGG + chr3_3:71487935-71487954 None:intergenic 40.0%
TGTTCTTGATCATCATGGTG+TGG + chr3_3:71487987-71488006 None:intergenic 40.0%
CACCATGATGATCAAGAACA+TGG - chr3_3:71487987-71488006 Msa0469570:three_prime_UTR 40.0%
TGCCATGTTCTTGATCATCA+TGG + chr3_3:71487992-71488011 None:intergenic 40.0%
AATCCACATACGGTTGTGAT+GGG - chr3_3:71488023-71488042 Msa0469570:three_prime_UTR 40.0%
ATTGTTAGTGTTTGTGGCTG+TGG + chr3_3:71488132-71488151 None:intergenic 40.0%
GTCGAGACATCTACATGTAT+AGG - chr3_3:71488261-71488280 Msa0469570:CDS 40.0%
TGTAAACCCTACTCTTCCTA+GGG - chr3_3:71488331-71488350 Msa0469570:intron 40.0%
!! ATTTTGCAGTTTGGAGTGCA+GGG - chr3_3:71488737-71488756 Msa0469570:intron 40.0%
! TGCTGCTTGTACTTGAAGAA+GGG - chr3_3:71488869-71488888 Msa0469570:intron 40.0%
CATGAATGACATTGTAAGCG+TGG + chr3_3:71488972-71488991 None:intergenic 40.0%
TTGTGCACTTACGACAAAGA+AGG - chr3_3:71489002-71489021 Msa0469570:CDS 40.0%
! TCCTTATGGCTCTTTTACAC+TGG - chr3_3:71489061-71489080 Msa0469570:CDS 40.0%
ACCAGTGTAAAAGAGCCATA+AGG + chr3_3:71489065-71489084 None:intergenic 40.0%
! AGAAGAACAAGAAGCATGAG+TGG - chr3_3:71487858-71487877 Msa0469570:three_prime_UTR 40.0%
!! TCTAGGTTGTTGGTTTGATC+AGG + chr3_3:71487909-71487928 None:intergenic 40.0%
! ATGATGGGATTCTAGGTTGT+TGG + chr3_3:71487919-71487938 None:intergenic 40.0%
!! CACTGATTCTTCCTCATGAT+GGG + chr3_3:71487934-71487953 None:intergenic 40.0%
!! TCACTGATTCTTCCTCATGA+TGG + chr3_3:71487935-71487954 None:intergenic 40.0%
TGTTCTTGATCATCATGGTG+TGG + chr3_3:71487987-71488006 None:intergenic 40.0%
CACCATGATGATCAAGAACA+TGG - chr3_3:71487987-71488006 Msa0469570:three_prime_UTR 40.0%
TGCCATGTTCTTGATCATCA+TGG + chr3_3:71487992-71488011 None:intergenic 40.0%
AATCCACATACGGTTGTGAT+GGG - chr3_3:71488023-71488042 Msa0469570:three_prime_UTR 40.0%
ATTGTTAGTGTTTGTGGCTG+TGG + chr3_3:71488132-71488151 None:intergenic 40.0%
GTCGAGACATCTACATGTAT+AGG - chr3_3:71488261-71488280 Msa0469570:CDS 40.0%
TGTAAACCCTACTCTTCCTA+GGG - chr3_3:71488331-71488350 Msa0469570:intron 40.0%
!! ATTTTGCAGTTTGGAGTGCA+GGG - chr3_3:71488737-71488756 Msa0469570:intron 40.0%
! TGCTGCTTGTACTTGAAGAA+GGG - chr3_3:71488869-71488888 Msa0469570:intron 40.0%
CATGAATGACATTGTAAGCG+TGG + chr3_3:71488972-71488991 None:intergenic 40.0%
TTGTGCACTTACGACAAAGA+AGG - chr3_3:71489002-71489021 Msa0469570:CDS 40.0%
! TCCTTATGGCTCTTTTACAC+TGG - chr3_3:71489061-71489080 Msa0469570:CDS 40.0%
ACCAGTGTAAAAGAGCCATA+AGG + chr3_3:71489065-71489084 None:intergenic 40.0%
! CTTCTCATAATTGGTGGTCG+AGG + chr3_3:71487843-71487862 None:intergenic 45.0%
! AGAACAAGAAGCATGAGTGG+TGG - chr3_3:71487861-71487880 Msa0469570:three_prime_UTR 45.0%
!! AGGTTGTTGGTTTGATCAGG+TGG + chr3_3:71487906-71487925 None:intergenic 45.0%
CAACCTAGAATCCCATCATG+AGG - chr3_3:71487920-71487939 Msa0469570:three_prime_UTR 45.0%
! CTTCCTCATGATGGGATTCT+AGG + chr3_3:71487926-71487945 None:intergenic 45.0%
TGATCATCATGGTGTGGATG+TGG + chr3_3:71487981-71488000 None:intergenic 45.0%
GAATCCACATACGGTTGTGA+TGG - chr3_3:71488022-71488041 Msa0469570:three_prime_UTR 45.0%
GTGGCTGATGAACGTTTAGT+GGG - chr3_3:71488077-71488096 Msa0469570:three_prime_UTR 45.0%
TCATGTGCACTCACACTCAA+AGG + chr3_3:71488176-71488195 None:intergenic 45.0%
CCACAAGTTTGGAGAAAGTG+AGG + chr3_3:71488215-71488234 None:intergenic 45.0%
CCTCACTTTCTCCAAACTTG+TGG - chr3_3:71488212-71488231 Msa0469570:CDS 45.0%
AGTTACAGAGGCCACAAGTT+TGG + chr3_3:71488226-71488245 None:intergenic 45.0%
TACATGTAGATGTCTCGACC+AGG + chr3_3:71488260-71488279 None:intergenic 45.0%
AGGAAGAGTAGGGTTTACAC+AGG + chr3_3:71488330-71488349 None:intergenic 45.0%
GTGTAAACCCTACTCTTCCT+AGG - chr3_3:71488330-71488349 Msa0469570:intron 45.0%
!! CATTTTGCAGTTTGGAGTGC+AGG - chr3_3:71488736-71488755 Msa0469570:intron 45.0%
TACACGGTGGTCTTCTTTGT+TGG + chr3_3:71488814-71488833 None:intergenic 45.0%
ACAAGCAGCAGTAGAAGCTT+TGG + chr3_3:71488859-71488878 None:intergenic 45.0%
! CTGCTGCTTGTACTTGAAGA+AGG - chr3_3:71488868-71488887 Msa0469570:intron 45.0%
TTGAGACAAAGGGAGAAACG+AGG + chr3_3:71489032-71489051 None:intergenic 45.0%
! CTTCTCATAATTGGTGGTCG+AGG + chr3_3:71487843-71487862 None:intergenic 45.0%
! AGAACAAGAAGCATGAGTGG+TGG - chr3_3:71487861-71487880 Msa0469570:three_prime_UTR 45.0%
!! AGGTTGTTGGTTTGATCAGG+TGG + chr3_3:71487906-71487925 None:intergenic 45.0%
CAACCTAGAATCCCATCATG+AGG - chr3_3:71487920-71487939 Msa0469570:three_prime_UTR 45.0%
! CTTCCTCATGATGGGATTCT+AGG + chr3_3:71487926-71487945 None:intergenic 45.0%
TGATCATCATGGTGTGGATG+TGG + chr3_3:71487981-71488000 None:intergenic 45.0%
GAATCCACATACGGTTGTGA+TGG - chr3_3:71488022-71488041 Msa0469570:three_prime_UTR 45.0%
GTGGCTGATGAACGTTTAGT+GGG - chr3_3:71488077-71488096 Msa0469570:three_prime_UTR 45.0%
TCATGTGCACTCACACTCAA+AGG + chr3_3:71488176-71488195 None:intergenic 45.0%
CCACAAGTTTGGAGAAAGTG+AGG + chr3_3:71488215-71488234 None:intergenic 45.0%
CCTCACTTTCTCCAAACTTG+TGG - chr3_3:71488212-71488231 Msa0469570:CDS 45.0%
AGTTACAGAGGCCACAAGTT+TGG + chr3_3:71488226-71488245 None:intergenic 45.0%
TACATGTAGATGTCTCGACC+AGG + chr3_3:71488260-71488279 None:intergenic 45.0%
AGGAAGAGTAGGGTTTACAC+AGG + chr3_3:71488330-71488349 None:intergenic 45.0%
GTGTAAACCCTACTCTTCCT+AGG - chr3_3:71488330-71488349 Msa0469570:intron 45.0%
!! CATTTTGCAGTTTGGAGTGC+AGG - chr3_3:71488736-71488755 Msa0469570:intron 45.0%
TACACGGTGGTCTTCTTTGT+TGG + chr3_3:71488814-71488833 None:intergenic 45.0%
ACAAGCAGCAGTAGAAGCTT+TGG + chr3_3:71488859-71488878 None:intergenic 45.0%
! CTGCTGCTTGTACTTGAAGA+AGG - chr3_3:71488868-71488887 Msa0469570:intron 45.0%
TTGAGACAAAGGGAGAAACG+AGG + chr3_3:71489032-71489051 None:intergenic 45.0%
ATCAGGTGGTTGCATGTCCA+CGG + chr3_3:71487892-71487911 None:intergenic 50.0%
GTTCCCATCACAACCGTATG+TGG + chr3_3:71488029-71488048 None:intergenic 50.0%
GGTGGCTGATGAACGTTTAG+TGG - chr3_3:71488076-71488095 Msa0469570:three_prime_UTR 50.0%
TGGCCTCTGTAACTGTCGTT+TGG - chr3_3:71488232-71488251 Msa0469570:CDS 50.0%
GAGCCAAACGACAGTTACAG+AGG + chr3_3:71488238-71488257 None:intergenic 50.0%
! TGTAACTGTCGTTTGGCTCC+TGG - chr3_3:71488239-71488258 Msa0469570:CDS 50.0%
AAGGCAGAGCCTTCGTAATC+CGG - chr3_3:71488355-71488374 Msa0469570:intron 50.0%
GACTGAACTCCGGATTACGA+AGG + chr3_3:71488367-71488386 None:intergenic 50.0%
! AGCAGTAGAAGCTTTGGCAG+TGG + chr3_3:71488853-71488872 None:intergenic 50.0%
AGACAAAGGGAGAAACGAGG+TGG + chr3_3:71489029-71489048 None:intergenic 50.0%
ATCAGGTGGTTGCATGTCCA+CGG + chr3_3:71487892-71487911 None:intergenic 50.0%
GTTCCCATCACAACCGTATG+TGG + chr3_3:71488029-71488048 None:intergenic 50.0%
GGTGGCTGATGAACGTTTAG+TGG - chr3_3:71488076-71488095 Msa0469570:three_prime_UTR 50.0%
TGGCCTCTGTAACTGTCGTT+TGG - chr3_3:71488232-71488251 Msa0469570:CDS 50.0%
GAGCCAAACGACAGTTACAG+AGG + chr3_3:71488238-71488257 None:intergenic 50.0%
! TGTAACTGTCGTTTGGCTCC+TGG - chr3_3:71488239-71488258 Msa0469570:CDS 50.0%
AAGGCAGAGCCTTCGTAATC+CGG - chr3_3:71488355-71488374 Msa0469570:intron 50.0%
GACTGAACTCCGGATTACGA+AGG + chr3_3:71488367-71488386 None:intergenic 50.0%
! AGCAGTAGAAGCTTTGGCAG+TGG + chr3_3:71488853-71488872 None:intergenic 50.0%
AGACAAAGGGAGAAACGAGG+TGG + chr3_3:71489029-71489048 None:intergenic 50.0%
! CATGAGTGGTGGTTTGTCCG+TGG - chr3_3:71487872-71487891 Msa0469570:three_prime_UTR 55.0%
TGGCTGTGGCTGTGTGTGAA+GGG + chr3_3:71488118-71488137 None:intergenic 55.0%
ACCCTACTCTTCCTAGGGCA+AGG - chr3_3:71488336-71488355 Msa0469570:intron 55.0%
GCCTTGCCCTAGGAAGAGTA+GGG + chr3_3:71488340-71488359 None:intergenic 55.0%
TGCCTTGCCCTAGGAAGAGT+AGG + chr3_3:71488341-71488360 None:intergenic 55.0%
! CATGAGTGGTGGTTTGTCCG+TGG - chr3_3:71487872-71487891 Msa0469570:three_prime_UTR 55.0%
TGGCTGTGGCTGTGTGTGAA+GGG + chr3_3:71488118-71488137 None:intergenic 55.0%
ACCCTACTCTTCCTAGGGCA+AGG - chr3_3:71488336-71488355 Msa0469570:intron 55.0%
GCCTTGCCCTAGGAAGAGTA+GGG + chr3_3:71488340-71488359 None:intergenic 55.0%
TGCCTTGCCCTAGGAAGAGT+AGG + chr3_3:71488341-71488360 None:intergenic 55.0%
TGGTGTGGATGTGGCTGCAG+AGG + chr3_3:71487972-71487991 None:intergenic 60.0%
GTGGCTGTGGCTGTGTGTGA+AGG + chr3_3:71488119-71488138 None:intergenic 60.0%
TGCACGGTGGTGATACACGG+TGG + chr3_3:71488827-71488846 None:intergenic 60.0%
CCGTGCACGGTGGTGATACA+CGG + chr3_3:71488830-71488849 None:intergenic 60.0%
CCGTGTATCACCACCGTGCA+CGG - chr3_3:71488827-71488846 Msa0469570:intron 60.0%
TGGTGTGGATGTGGCTGCAG+AGG + chr3_3:71487972-71487991 None:intergenic 60.0%
GTGGCTGTGGCTGTGTGTGA+AGG + chr3_3:71488119-71488138 None:intergenic 60.0%
TGCACGGTGGTGATACACGG+TGG + chr3_3:71488827-71488846 None:intergenic 60.0%
CCGTGCACGGTGGTGATACA+CGG + chr3_3:71488830-71488849 None:intergenic 60.0%
CCGTGTATCACCACCGTGCA+CGG - chr3_3:71488827-71488846 Msa0469570:intron 60.0%
CGAAGGCTCTGCCTTGCCCT+AGG + chr3_3:71488350-71488369 None:intergenic 65.0%
GCTTTGGCAGTGGCCGTGCA+CGG + chr3_3:71488843-71488862 None:intergenic 65.0%
CGAAGGCTCTGCCTTGCCCT+AGG + chr3_3:71488350-71488369 None:intergenic 65.0%
GCTTTGGCAGTGGCCGTGCA+CGG + chr3_3:71488843-71488862 None:intergenic 65.0%
TTGGCAGTGGCCGTGCACGG+TGG + chr3_3:71488840-71488859 None:intergenic 70.0%
TTGGCAGTGGCCGTGCACGG+TGG + chr3_3:71488840-71488859 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 71487822 71489245 71487822 ID=Msa0469570;Name=Msa0469570
chr3_3 mRNA 71487822 71489245 71487822 ID=Msa0469570-mRNA-1;Parent=Msa0469570;Name=Msa0469570-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0|0.5|1|1|1|2|361|149
chr3_3 exon 71487822 71488342 71487822 ID=Msa0469570-mRNA-1:exon:17549;Parent=Msa0469570-mRNA-1
chr3_3 exon 71488956 71489245 71488956 ID=Msa0469570-mRNA-1:exon:17548;Parent=Msa0469570-mRNA-1
chr3_3 CDS 71488956 71489245 71488956 ID=Msa0469570-mRNA-1:cds;Parent=Msa0469570-mRNA-1
chr3_3 CDS 71488183 71488342 71488183 ID=Msa0469570-mRNA-1:cds;Parent=Msa0469570-mRNA-1
chr3_3 three_prime_UTR 71487822 71488182 71487822 ID=Msa0469570-mRNA-1:three_prime_utr;Parent=Msa0469570-mRNA-1
Gene Sequence

>Msa0469570

ATGTTGCTTGGAAAACGTCCTCGACCACCAATTATGAGAAGAACAAGAAGCATGAGTGGTGGTTTGTCCGTGGACATGCAACCACCTGATCAAACCAACAACCTAGAATCCCATCATGAGGAAGAATCAGTGATGTCTCATCAGAATCCTCTGCAGCCACATCCACACCATGATGATCAAGAACATGGCATCAACATCAAGAATCCACATACGGTTGTGATGGGAACAGAAACACACATTAAAAGTGAATTAACGGTGGCTGATGAACGTTTAGTGGGTAGTGCAGTCATGGGAGATACAGCATTTTGCAGTTTGGAGTGCAGGGAGCAACAAATAAAACAAGACAAGAGAAAAGAAAAGTGGAAGATGGCGTTCACCAACAAAGAAGACCACCGTGTATCACCACCGTGCACGGCCACTGCCAAAGCTTCTACTGCTGCTTGTACTTGA
Protein sequence

>Msa0469570

MLLGKRPRPPIMRRTRSMSGGLSVDMQPPDQTNNLESHHEEESVMSHQNPLQPHPHHDDQEHGINIKNPHTVVMGTETHIKSELTVADERLVGSAVMGDTAFCSLECREQQIKQDKRKEKWKMAFTNKEDHRVSPPCTATAKASTAACT*