AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0518890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0518890 MtrunA17_Chr3g0131211 92.638 163 12 0 1 163 1 163 9.09e-109 306
Msa0518890 MtrunA17_Chr3g0131241 71.698 159 42 1 1 159 1 156 3.78e-82 239
Msa0518890 MtrunA17_Chr3g0131251 69.325 163 47 1 1 163 1 160 2.42e-78 229
Msa0518890 MtrunA17_Chr3g0131201 65.031 163 48 3 1 163 43 196 3.41e-68 205
Msa0518890 MtrunA17_Chr3g0131231 65.278 144 33 2 20 163 9 135 1.65e-65 196
Msa0518890 MtrunA17_Chr3g0131191 61.290 155 58 2 10 163 2 155 8.04e-63 190
Msa0518890 MtrunA17_Chr3g0131261 63.551 107 39 0 9 115 4 110 8.63e-47 149
Msa0518890 MtrunA17_Chr3g0131221 48.077 104 44 2 60 163 1 94 5.52e-21 82.0
Msa0518890 MtrunA17_Chr5g0429471 39.062 128 74 3 37 163 37 161 7.53e-20 80.9
Msa0518890 MtrunA17_Chr8g0350041 27.559 127 92 0 37 163 61 187 8.48e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0518890 AT1G04290.1 65.333 150 48 3 10 157 2 149 8.00e-61 185
Msa0518890 AT1G04290.2 62.385 109 37 3 10 116 2 108 3.98e-36 122
Msa0518890 AT2G29590.1 35.758 165 86 7 11 163 2 158 3.75e-17 74.3

Find 34 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATGGAGTTCAGGAGTTT+CGG 0.344275 3_4:-70756852 Msa0518890:CDS
CATCACTACTTTGGTGGATC+TGG 0.357320 3_4:-70756906 Msa0518890:CDS
CTGTTATAAGCGTGGAGTTA+AGG 0.385303 3_4:-70756466 Msa0518890:CDS
AGGAGAAGAATCAGCATCAA+TGG 0.446669 3_4:-70757156 Msa0518890:CDS
AAGAGGTACTTGGAGAAGAA+AGG 0.461866 3_4:-70757176 Msa0518890:CDS
ACTGATAAAGGGAATATACT+TGG 0.473510 3_4:+70756403 None:intergenic
AGAATCAGTGAAGAGGTACT+TGG 0.475261 3_4:-70757186 Msa0518890:CDS
CGAGATCAATGTCTCATGCT+TGG 0.500260 3_4:-70756828 Msa0518890:CDS
ATTGAGAAAACGATGCGGCC+AGG 0.510535 3_4:+70757062 None:intergenic
TGGTGCCATCACTACTTTGG+TGG 0.522618 3_4:-70756912 Msa0518890:CDS
GCAATGGCAGTTCCCACTGC+GGG 0.542837 3_4:-70756878 Msa0518890:CDS
CGGTGGTGCCATCACTACTT+TGG 0.548373 3_4:-70756915 Msa0518890:CDS
GGTAGACGATGAATTGCCTC+GGG 0.567000 3_4:-70757135 Msa0518890:CDS
TGGTAGACGATGAATTGCCT+CGG 0.567173 3_4:-70757136 Msa0518890:CDS
GGCCAGGTTGTATGAGATCA+AGG 0.567753 3_4:+70757078 None:intergenic
CACCTTGATCTCATACAACC+TGG 0.575066 3_4:-70757080 Msa0518890:CDS
GGTGGATCTGGTAGGCGCAA+TGG 0.579055 3_4:-70756894 Msa0518890:CDS
GACTATAGCTGTTATAAGCG+TGG 0.583205 3_4:-70756474 Msa0518890:CDS
TTGGGACATCTAGTCGTAGG+TGG 0.586154 3_4:-70757107 Msa0518890:CDS
AAGATACGAACGAGTAAATG+TGG 0.592245 3_4:+70757029 None:intergenic
CTATGGTGAAAACAGAAGAA+AGG 0.596425 3_4:-70757220 Msa0518890:CDS
CAGATCCACCAAAGTAGTGA+TGG 0.597903 3_4:+70756907 None:intergenic
CTCCATGAAAATCCCGCAGT+GGG 0.600089 3_4:+70756866 None:intergenic
ACTACTTTGGTGGATCTGGT+AGG 0.605004 3_4:-70756902 Msa0518890:CDS
GAAAACAGAAGAAAGGATGA+CGG 0.627478 3_4:-70757213 Msa0518890:CDS
CGGATTTAGAATCAGTGAAG+AGG 0.636860 3_4:-70757193 Msa0518890:CDS
TAGGAGGAGATTGAGATCGA+TGG 0.637595 3_4:-70756518 Msa0518890:CDS
AATTCAAGTAACTACTTACA+CGG 0.637870 3_4:-70756935 Msa0518890:CDS
GATGGCAGAGTTCTACGAGT+AGG 0.654230 3_4:-70756500 Msa0518890:CDS
CGCAATGGCAGTTCCCACTG+CGG 0.659015 3_4:-70756879 Msa0518890:CDS
ACTCCATGAAAATCCCGCAG+TGG 0.688697 3_4:+70756865 None:intergenic
ATACGAACGAGTAAATGTGG+AGG 0.729121 3_4:+70757032 None:intergenic
TCTTCATTGAGAAAACGATG+CGG 0.782337 3_4:+70757057 None:intergenic
TCAAGTAACTACTTACACGG+TGG 0.786690 3_4:-70756932 Msa0518890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTAAAATTCTCTA+TGG - chr3_4:70757059-70757078 Msa0518890:CDS 10.0%
!!! TTATTTTTTAAAATTCTCTA+TGG - chr3_4:70757059-70757078 Msa0518890:CDS 10.0%
!! ATCCATTAATGTTATTATTA+GGG + chr3_4:70756632-70756651 None:intergenic 15.0%
!! AATCCATTAATGTTATTATT+AGG + chr3_4:70756633-70756652 None:intergenic 15.0%
!! ATCCATTAATGTTATTATTA+GGG + chr3_4:70756632-70756651 None:intergenic 15.0%
!! AATCCATTAATGTTATTATT+AGG + chr3_4:70756633-70756652 None:intergenic 15.0%
!! TTCCCTAATAATAACATTAA+TGG - chr3_4:70756627-70756646 Msa0518890:intron 20.0%
!!! TTCTTTTCTTGGTAAAATAT+TGG - chr3_4:70757007-70757026 Msa0518890:intron 20.0%
!! TTCCCTAATAATAACATTAA+TGG - chr3_4:70756627-70756646 Msa0518890:intron 20.0%
!!! TTCTTTTCTTGGTAAAATAT+TGG - chr3_4:70757007-70757026 Msa0518890:intron 20.0%
! AATTCAAGTAACTACTTACA+CGG - chr3_4:70756677-70756696 Msa0518890:intron 25.0%
!!! TTGTGTTGGTTAATCAATTA+TGG + chr3_4:70756941-70756960 None:intergenic 25.0%
! TTCCTCAAATTATGTAGTAT+CGG + chr3_4:70756980-70756999 None:intergenic 25.0%
! AATTCAAGTAACTACTTACA+CGG - chr3_4:70756677-70756696 Msa0518890:intron 25.0%
!!! TTGTGTTGGTTAATCAATTA+TGG + chr3_4:70756941-70756960 None:intergenic 25.0%
! TTCCTCAAATTATGTAGTAT+CGG + chr3_4:70756980-70756999 None:intergenic 25.0%
!! TTGGTTTGTTACTAAGTGTT+GGG + chr3_4:70756915-70756934 None:intergenic 30.0%
!! ATTGGTTTGTTACTAAGTGT+TGG + chr3_4:70756916-70756935 None:intergenic 30.0%
GGTTAATCAATTATGGTCAT+TGG + chr3_4:70756934-70756953 None:intergenic 30.0%
ATCACTCATTTACTTTGTGT+TGG + chr3_4:70756955-70756974 None:intergenic 30.0%
ATCCGATACTACATAATTTG+AGG - chr3_4:70756975-70756994 Msa0518890:intron 30.0%
! GGAATATGAGATTCTTTTCT+TGG - chr3_4:70756996-70757015 Msa0518890:intron 30.0%
GAGTTAAGGAAGAAAAAAAC+TGG - chr3_4:70757160-70757179 Msa0518890:CDS 30.0%
ACTGATAAAGGGAATATACT+TGG + chr3_4:70757212-70757231 None:intergenic 30.0%
!! TTGGTTTGTTACTAAGTGTT+GGG + chr3_4:70756915-70756934 None:intergenic 30.0%
!! ATTGGTTTGTTACTAAGTGT+TGG + chr3_4:70756916-70756935 None:intergenic 30.0%
GGTTAATCAATTATGGTCAT+TGG + chr3_4:70756934-70756953 None:intergenic 30.0%
ATCACTCATTTACTTTGTGT+TGG + chr3_4:70756955-70756974 None:intergenic 30.0%
ATCCGATACTACATAATTTG+AGG - chr3_4:70756975-70756994 Msa0518890:intron 30.0%
! GGAATATGAGATTCTTTTCT+TGG - chr3_4:70756996-70757015 Msa0518890:intron 30.0%
GAGTTAAGGAAGAAAAAAAC+TGG - chr3_4:70757160-70757179 Msa0518890:CDS 30.0%
ACTGATAAAGGGAATATACT+TGG + chr3_4:70757212-70757231 None:intergenic 30.0%
GAAAACAGAAGAAAGGATGA+CGG - chr3_4:70756399-70756418 Msa0518890:CDS 35.0%
TCTTCATTGAGAAAACGATG+CGG + chr3_4:70756558-70756577 None:intergenic 35.0%
AAGATACGAACGAGTAAATG+TGG + chr3_4:70756586-70756605 None:intergenic 35.0%
TCTATGGCTTATGTGATGAT+AGG - chr3_4:70757075-70757094 Msa0518890:CDS 35.0%
GAAAACAGAAGAAAGGATGA+CGG - chr3_4:70756399-70756418 Msa0518890:CDS 35.0%
TCTTCATTGAGAAAACGATG+CGG + chr3_4:70756558-70756577 None:intergenic 35.0%
AAGATACGAACGAGTAAATG+TGG + chr3_4:70756586-70756605 None:intergenic 35.0%
TCTATGGCTTATGTGATGAT+AGG - chr3_4:70757075-70757094 Msa0518890:CDS 35.0%
! CGGATTTAGAATCAGTGAAG+AGG - chr3_4:70756419-70756438 Msa0518890:CDS 40.0%
!! AGAATCAGTGAAGAGGTACT+TGG - chr3_4:70756426-70756445 Msa0518890:CDS 40.0%
!! AAGAGGTACTTGGAGAAGAA+AGG - chr3_4:70756436-70756455 Msa0518890:CDS 40.0%
AGGAGAAGAATCAGCATCAA+TGG - chr3_4:70756456-70756475 Msa0518890:CDS 40.0%
!!! TTTTTGGGACATCTAGTCGT+AGG - chr3_4:70756502-70756521 Msa0518890:CDS 40.0%
ATACGAACGAGTAAATGTGG+AGG + chr3_4:70756583-70756602 None:intergenic 40.0%
TCAAGTAACTACTTACACGG+TGG - chr3_4:70756680-70756699 Msa0518890:intron 40.0%
TTCATGGAGTTCAGGAGTTT+CGG - chr3_4:70756760-70756779 Msa0518890:intron 40.0%
ATGGCTTATGTGATGATAGG+AGG - chr3_4:70757078-70757097 Msa0518890:CDS 40.0%
GACTATAGCTGTTATAAGCG+TGG - chr3_4:70757138-70757157 Msa0518890:CDS 40.0%
CTGTTATAAGCGTGGAGTTA+AGG - chr3_4:70757146-70757165 Msa0518890:CDS 40.0%
! AACTGGCCAAATTTTTGCTC+AGG - chr3_4:70757177-70757196 Msa0518890:CDS 40.0%
! ACTGGCCAAATTTTTGCTCA+GGG - chr3_4:70757178-70757197 Msa0518890:CDS 40.0%
! CGGATTTAGAATCAGTGAAG+AGG - chr3_4:70756419-70756438 Msa0518890:CDS 40.0%
!! AGAATCAGTGAAGAGGTACT+TGG - chr3_4:70756426-70756445 Msa0518890:CDS 40.0%
!! AAGAGGTACTTGGAGAAGAA+AGG - chr3_4:70756436-70756455 Msa0518890:CDS 40.0%
AGGAGAAGAATCAGCATCAA+TGG - chr3_4:70756456-70756475 Msa0518890:CDS 40.0%
!!! TTTTTGGGACATCTAGTCGT+AGG - chr3_4:70756502-70756521 Msa0518890:CDS 40.0%
ATACGAACGAGTAAATGTGG+AGG + chr3_4:70756583-70756602 None:intergenic 40.0%
TCAAGTAACTACTTACACGG+TGG - chr3_4:70756680-70756699 Msa0518890:intron 40.0%
TTCATGGAGTTCAGGAGTTT+CGG - chr3_4:70756760-70756779 Msa0518890:intron 40.0%
ATGGCTTATGTGATGATAGG+AGG - chr3_4:70757078-70757097 Msa0518890:CDS 40.0%
GACTATAGCTGTTATAAGCG+TGG - chr3_4:70757138-70757157 Msa0518890:CDS 40.0%
CTGTTATAAGCGTGGAGTTA+AGG - chr3_4:70757146-70757165 Msa0518890:CDS 40.0%
! AACTGGCCAAATTTTTGCTC+AGG - chr3_4:70757177-70757196 Msa0518890:CDS 40.0%
! ACTGGCCAAATTTTTGCTCA+GGG - chr3_4:70757178-70757197 Msa0518890:CDS 40.0%
TGGTAGACGATGAATTGCCT+CGG - chr3_4:70756476-70756495 Msa0518890:CDS 45.0%
! TGAATTGCCTCGGGAGTTTT+TGG - chr3_4:70756486-70756505 Msa0518890:CDS 45.0%
! GAATTGCCTCGGGAGTTTTT+GGG - chr3_4:70756487-70756506 Msa0518890:CDS 45.0%
CACCTTGATCTCATACAACC+TGG - chr3_4:70756532-70756551 Msa0518890:intron 45.0%
CAGATCCACCAAAGTAGTGA+TGG + chr3_4:70756708-70756727 None:intergenic 45.0%
! CATCACTACTTTGGTGGATC+TGG - chr3_4:70756706-70756725 Msa0518890:intron 45.0%
! ACTACTTTGGTGGATCTGGT+AGG - chr3_4:70756710-70756729 Msa0518890:intron 45.0%
CGAGATCAATGTCTCATGCT+TGG - chr3_4:70756784-70756803 Msa0518890:intron 45.0%
TAGGAGGAGATTGAGATCGA+TGG - chr3_4:70757094-70757113 Msa0518890:CDS 45.0%
! CTGGCCAAATTTTTGCTCAG+GGG - chr3_4:70757179-70757198 Msa0518890:CDS 45.0%
TGGTAGACGATGAATTGCCT+CGG - chr3_4:70756476-70756495 Msa0518890:CDS 45.0%
! TGAATTGCCTCGGGAGTTTT+TGG - chr3_4:70756486-70756505 Msa0518890:CDS 45.0%
! GAATTGCCTCGGGAGTTTTT+GGG - chr3_4:70756487-70756506 Msa0518890:CDS 45.0%
CACCTTGATCTCATACAACC+TGG - chr3_4:70756532-70756551 Msa0518890:intron 45.0%
CAGATCCACCAAAGTAGTGA+TGG + chr3_4:70756708-70756727 None:intergenic 45.0%
! CATCACTACTTTGGTGGATC+TGG - chr3_4:70756706-70756725 Msa0518890:intron 45.0%
! ACTACTTTGGTGGATCTGGT+AGG - chr3_4:70756710-70756729 Msa0518890:intron 45.0%
CGAGATCAATGTCTCATGCT+TGG - chr3_4:70756784-70756803 Msa0518890:intron 45.0%
TAGGAGGAGATTGAGATCGA+TGG - chr3_4:70757094-70757113 Msa0518890:CDS 45.0%
! CTGGCCAAATTTTTGCTCAG+GGG - chr3_4:70757179-70757198 Msa0518890:CDS 45.0%
GGTAGACGATGAATTGCCTC+GGG - chr3_4:70756477-70756496 Msa0518890:CDS 50.0%
AGATGTCCCAAAAACTCCCG+AGG + chr3_4:70756496-70756515 None:intergenic 50.0%
TTGGGACATCTAGTCGTAGG+TGG - chr3_4:70756505-70756524 Msa0518890:CDS 50.0%
GGCCAGGTTGTATGAGATCA+AGG + chr3_4:70756537-70756556 None:intergenic 50.0%
ATTGAGAAAACGATGCGGCC+AGG + chr3_4:70756553-70756572 None:intergenic 50.0%
!! TGGTGCCATCACTACTTTGG+TGG - chr3_4:70756700-70756719 Msa0518890:intron 50.0%
! TTCCCACTGCGGGATTTTCA+TGG - chr3_4:70756744-70756763 Msa0518890:intron 50.0%
CTCCATGAAAATCCCGCAGT+GGG + chr3_4:70756749-70756768 None:intergenic 50.0%
ACTCCATGAAAATCCCGCAG+TGG + chr3_4:70756750-70756769 None:intergenic 50.0%
! GCGGGATTTTCATGGAGTTC+AGG - chr3_4:70756752-70756771 Msa0518890:intron 50.0%
GATGGCAGAGTTCTACGAGT+AGG - chr3_4:70757112-70757131 Msa0518890:CDS 50.0%
GACGCCCCTGAGCAAAAATT+TGG + chr3_4:70757186-70757205 None:intergenic 50.0%
GGTAGACGATGAATTGCCTC+GGG - chr3_4:70756477-70756496 Msa0518890:CDS 50.0%
AGATGTCCCAAAAACTCCCG+AGG + chr3_4:70756496-70756515 None:intergenic 50.0%
TTGGGACATCTAGTCGTAGG+TGG - chr3_4:70756505-70756524 Msa0518890:CDS 50.0%
GGCCAGGTTGTATGAGATCA+AGG + chr3_4:70756537-70756556 None:intergenic 50.0%
ATTGAGAAAACGATGCGGCC+AGG + chr3_4:70756553-70756572 None:intergenic 50.0%
!! TGGTGCCATCACTACTTTGG+TGG - chr3_4:70756700-70756719 Msa0518890:intron 50.0%
! TTCCCACTGCGGGATTTTCA+TGG - chr3_4:70756744-70756763 Msa0518890:intron 50.0%
CTCCATGAAAATCCCGCAGT+GGG + chr3_4:70756749-70756768 None:intergenic 50.0%
ACTCCATGAAAATCCCGCAG+TGG + chr3_4:70756750-70756769 None:intergenic 50.0%
! GCGGGATTTTCATGGAGTTC+AGG - chr3_4:70756752-70756771 Msa0518890:intron 50.0%
GATGGCAGAGTTCTACGAGT+AGG - chr3_4:70757112-70757131 Msa0518890:CDS 50.0%
GACGCCCCTGAGCAAAAATT+TGG + chr3_4:70757186-70757205 None:intergenic 50.0%
!! CGGTGGTGCCATCACTACTT+TGG - chr3_4:70756697-70756716 Msa0518890:intron 55.0%
!! CGGTGGTGCCATCACTACTT+TGG - chr3_4:70756697-70756716 Msa0518890:intron 55.0%
GGTGGATCTGGTAGGCGCAA+TGG - chr3_4:70756718-70756737 Msa0518890:intron 60.0%
CGCAATGGCAGTTCCCACTG+CGG - chr3_4:70756733-70756752 Msa0518890:intron 60.0%
GCAATGGCAGTTCCCACTGC+GGG - chr3_4:70756734-70756753 Msa0518890:intron 60.0%
GGTGGATCTGGTAGGCGCAA+TGG - chr3_4:70756718-70756737 Msa0518890:intron 60.0%
CGCAATGGCAGTTCCCACTG+CGG - chr3_4:70756733-70756752 Msa0518890:intron 60.0%
GCAATGGCAGTTCCCACTGC+GGG - chr3_4:70756734-70756753 Msa0518890:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 70756394 70757240 70756394 ID=Msa0518890;Name=Msa0518890
chr3_4 mRNA 70756394 70757240 70756394 ID=Msa0518890-mRNA-1;Parent=Msa0518890;Name=Msa0518890-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|3|0|163
chr3_4 exon 70757040 70757240 70757040 ID=Msa0518890-mRNA-1:exon:19354;Parent=Msa0518890-mRNA-1
chr3_4 exon 70756811 70756957 70756811 ID=Msa0518890-mRNA-1:exon:19353;Parent=Msa0518890-mRNA-1
chr3_4 exon 70756394 70756537 70756394 ID=Msa0518890-mRNA-1:exon:19352;Parent=Msa0518890-mRNA-1
chr3_4 CDS 70757040 70757240 70757040 ID=Msa0518890-mRNA-1:cds;Parent=Msa0518890-mRNA-1
chr3_4 CDS 70756811 70756957 70756811 ID=Msa0518890-mRNA-1:cds;Parent=Msa0518890-mRNA-1
chr3_4 CDS 70756394 70756537 70756394 ID=Msa0518890-mRNA-1:cds;Parent=Msa0518890-mRNA-1
Gene Sequence

>Msa0518890

ATGGTGAAAACAGAAGAAAGGATGACGGATTTAGAATCAGTGAAGAGGTACTTGGAGAAGAAAGGAGAAGAATCAGCATCAATGGTAGACGATGAATTGCCTCGGGAGTTTTTGGGACATCTAGTCGTAGGTGGCTTACACCTTGATCTCATACAACCTGGCCGCATCGTTTTCTCAATGAAGATACCTCCACATTTACTCAATTCAAGTAACTACTTACACGGTGGTGCCATCACTACTTTGGTGGATCTGGTAGGCGCAATGGCAGTTCCCACTGCGGGATTTTCATGGAGTTCAGGAGTTTCGGTCGAGATCAATGTCTCATGCTTGGATGCTGCATATGTCGATGAGGAGATTGAGATCGATGGCAGAGTTCTACGAGTAGGAAAGACTATAGCTGTTATAAGCGTGGAGTTAAGGAAGAAAAAAACTGGCCAAATTTTTGCTCAGGGGCGTCATACCAAGTATATTCCCTTTATCAGTAAAATGTGA

Protein sequence

>Msa0518890

MVKTEERMTDLESVKRYLEKKGEESASMVDDELPREFLGHLVVGGLHLDLIQPGRIVFSMKIPPHLLNSSNYLHGGAITTLVDLVGAMAVPTAGFSWSSGVSVEINVSCLDAAYVDEEIEIDGRVLRVGKTIAVISVELRKKKTGQIFAQGRHTKYIPFISKM*