AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0523500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0523500 MtrunA17_Chr4g0035001 84.337 83 13 0 1 83 1 83 2.06e-48 153
Msa0523500 MtrunA17_Chr4g0035051 82.927 82 14 0 2 83 4 85 9.27e-48 150
Msa0523500 MtrunA17_Chr4g0035031 80.723 83 16 0 2 84 4 86 6.66e-45 145
Msa0523500 MtrunA17_Chr2g0278661 60.241 83 33 0 5 87 6 88 9.03e-33 115
Msa0523500 MtrunA17_Chr4g0050781 59.036 83 34 0 3 85 4 86 6.60e-32 112
Msa0523500 MtrunA17_Chr3g0121631 53.165 79 36 1 7 85 37 114 8.09e-25 92.8
Msa0523500 MtrunA17_Chr3g0116631 52.273 88 42 0 3 90 203 290 3.29e-24 94.7
Msa0523500 MtrunA17_Chr3g0116631 41.975 81 47 0 7 87 110 190 1.92e-13 65.5
Msa0523500 MtrunA17_Chr4g0061451 48.101 79 41 0 7 85 43 121 1.53e-23 92.4
Msa0523500 MtrunA17_Chr7g0219021 44.578 83 46 0 7 89 41 123 3.70e-23 91.3
Msa0523500 MtrunA17_Chr1g0210241 49.383 81 41 0 5 85 6 86 4.59e-23 91.3
Msa0523500 MtrunA17_Chr3g0086861 46.835 79 42 0 7 85 45 123 1.25e-22 92.0
Msa0523500 MtrunA17_Chr3g0119311 46.341 82 44 0 7 88 37 118 2.59e-22 85.9
Msa0523500 MtrunA17_Chr4g0058151 51.852 81 39 0 6 86 216 296 5.29e-22 89.0
Msa0523500 MtrunA17_Chr4g0058151 38.235 102 63 0 5 106 121 222 6.92e-17 75.1
Msa0523500 MtrunA17_Chr1g0181111 55.000 80 36 0 7 86 197 276 6.62e-22 88.2
Msa0523500 MtrunA17_Chr1g0181111 35.878 131 77 2 7 137 85 208 1.24e-11 60.5
Msa0523500 MtrunA17_Chr7g0263001 49.367 79 40 0 7 85 36 114 8.20e-20 79.7
Msa0523500 MtrunA17_Chr7g0266751 43.210 81 46 0 4 84 30 110 3.38e-19 78.2
Msa0523500 MtrunA17_Chr4g0017401 45.263 95 44 3 7 95 17 109 1.00e-18 79.7
Msa0523500 MtrunA17_Chr6g0481071 43.750 80 45 0 7 86 96 175 9.09e-18 75.5
Msa0523500 MtrunA17_Chr1g0168601 43.902 82 46 0 2 83 149 230 1.03e-17 76.3
Msa0523500 MtrunA17_Chr4g0042161 43.820 89 50 0 1 89 46 134 3.48e-17 73.2
Msa0523500 MtrunA17_Chr8g0346011 33.077 130 71 2 7 121 38 166 7.16e-17 75.1
Msa0523500 MtrunA17_Chr3g0144311 46.053 76 40 1 7 82 30 104 1.27e-16 74.3
Msa0523500 MtrunA17_Chr3g0127751 32.117 137 79 3 7 132 27 160 1.29e-16 73.9
Msa0523500 MtrunA17_Chr3g0101061 39.241 79 46 1 7 85 41 117 4.33e-16 73.9
Msa0523500 MtrunA17_Chr2g0327921 39.024 82 50 0 7 88 8 89 4.46e-16 68.6
Msa0523500 MtrunA17_Chr7g0219031 42.424 66 38 0 7 72 41 106 1.21e-15 68.2
Msa0523500 MtrunA17_Chr5g0447761 39.241 79 46 2 7 85 7 83 1.40e-14 69.3
Msa0523500 MtrunA17_Chr1g0198431 37.500 88 55 0 7 94 193 280 1.61e-14 68.6
Msa0523500 MtrunA17_Chr3g0110211 43.373 83 46 1 8 89 114 196 2.05e-14 68.9
Msa0523500 MtrunA17_Chr5g0426311 42.105 76 43 1 7 82 17 91 2.25e-14 68.2
Msa0523500 MtrunA17_Chr2g0290191 36.709 79 48 1 7 85 5 81 2.67e-14 68.6
Msa0523500 MtrunA17_Chr1g0186351 35.443 79 49 1 7 85 7 83 2.78e-14 68.6
Msa0523500 MtrunA17_Chr1g0186351 35.294 85 54 1 7 91 113 196 2.65e-13 65.9
Msa0523500 MtrunA17_Chr3g0088511 40.541 74 44 0 7 80 33 106 4.80e-14 64.7
Msa0523500 MtrunA17_Chr3g0120721 33.582 134 80 3 7 134 107 237 5.95e-14 67.4
Msa0523500 MtrunA17_Chr4g0031091 41.096 73 43 0 7 79 34 106 7.51e-14 63.5
Msa0523500 MtrunA17_Chr7g0276531 47.458 59 31 0 7 65 19 77 9.19e-14 66.2
Msa0523500 MtrunA17_Chr4g0065741 35.165 91 58 1 7 97 72 161 8.61e-13 62.0
Msa0523500 MtrunA17_Chr6g0449481 30.392 102 70 1 7 107 134 235 9.45e-13 63.9
Msa0523500 MtrunA17_Chr7g0267571 36.047 86 55 0 6 91 212 297 1.55e-12 63.2
Msa0523500 MtrunA17_Chr4g0038111 36.458 96 59 2 9 104 342 435 2.50e-12 63.2
Msa0523500 MtrunA17_Chr5g0419351 39.535 86 52 0 8 93 12 97 8.32e-12 59.7
Msa0523500 MtrunA17_Chr4g0062931 33.600 125 81 2 8 132 9 131 8.94e-12 61.6
Msa0523500 MtrunA17_Chr7g0265341 43.836 73 40 1 9 81 161 232 2.00e-11 60.5
Msa0523500 MtrunA17_Chr4g0044541 42.857 84 44 2 9 89 125 207 2.65e-11 60.1
Msa0523500 MtrunA17_Chr8g0360551 38.554 83 49 2 7 89 74 154 4.23e-11 59.3
Msa0523500 MtrunA17_Chr8g0344491 38.824 85 51 1 5 89 146 229 4.57e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0523500 AT4G39260.3 74.725 91 23 0 1 91 1 91 1.80e-46 146
Msa0523500 AT2G21660.1 79.012 81 17 0 3 83 5 85 2.55e-43 141
Msa0523500 AT2G21660.2 77.083 96 22 0 3 98 5 100 7.82e-42 136
Msa0523500 AT4G39260.1 72.289 83 23 0 1 83 1 83 1.64e-40 134
Msa0523500 AT4G39260.2 73.469 98 24 1 1 98 1 96 5.99e-38 125
Msa0523500 AT3G26420.1 53.465 101 47 0 3 103 4 104 5.70e-33 116
Msa0523500 AT4G39260.4 70.588 68 20 0 1 68 1 68 3.48e-32 110
Msa0523500 AT3G23830.2 57.317 82 35 0 7 88 36 117 3.06e-28 101
Msa0523500 AT3G23830.1 57.317 82 35 0 7 88 36 117 3.06e-28 101
Msa0523500 AT4G13850.3 54.878 82 37 0 7 88 36 117 5.01e-26 96.3
Msa0523500 AT4G13850.4 55.556 81 36 0 7 87 36 116 5.15e-26 95.5
Msa0523500 AT4G13850.2 54.878 82 37 0 7 88 36 117 6.35e-26 96.3
Msa0523500 AT4G13850.1 54.878 82 37 0 7 88 36 117 7.24e-26 96.3
Msa0523500 AT3G53460.1 51.807 83 40 0 7 89 258 340 1.60e-23 94.0
Msa0523500 AT3G53460.1 42.105 76 44 0 5 80 98 173 5.86e-11 59.3
Msa0523500 AT3G53460.3 51.807 83 40 0 7 89 258 340 1.60e-23 94.0
Msa0523500 AT3G53460.3 42.105 76 44 0 5 80 98 173 5.86e-11 59.3
Msa0523500 AT3G53460.2 51.807 83 40 0 7 89 258 340 1.60e-23 94.0
Msa0523500 AT3G53460.2 42.105 76 44 0 5 80 98 173 5.86e-11 59.3
Msa0523500 AT2G37220.1 50.617 81 40 0 7 87 205 285 1.99e-23 92.8
Msa0523500 AT2G37220.1 43.421 76 43 0 5 80 90 165 5.45e-13 64.7
Msa0523500 AT3G53460.4 51.807 83 40 0 7 89 279 361 2.28e-23 94.0
Msa0523500 AT4G24770.1 50.588 85 42 0 3 87 241 325 9.41e-23 91.7
Msa0523500 AT4G24770.1 40.196 102 61 0 5 106 149 250 1.44e-16 75.1
Msa0523500 AT4G24770.2 50.588 85 42 0 3 87 241 325 9.41e-23 91.7
Msa0523500 AT4G24770.2 40.196 102 61 0 5 106 149 250 1.44e-16 75.1
Msa0523500 AT5G61030.2 54.348 92 42 0 7 98 41 132 1.40e-22 90.9
Msa0523500 AT5G61030.1 54.348 92 42 0 7 98 41 132 1.40e-22 90.9
Msa0523500 AT5G06210.1 44.444 90 50 0 4 93 32 121 2.26e-21 84.3
Msa0523500 AT5G04280.1 45.161 93 51 0 7 99 8 100 2.50e-21 87.8
Msa0523500 AT1G74230.1 52.632 76 35 1 7 82 35 109 5.34e-21 86.3
Msa0523500 AT5G50250.1 50.000 80 40 0 6 85 207 286 5.99e-21 86.3
Msa0523500 AT5G50250.1 39.216 102 62 0 5 106 112 213 3.54e-16 73.6
Msa0523500 AT1G18630.1 50.000 78 39 0 7 84 37 114 1.11e-20 82.8
Msa0523500 AT5G47320.1 49.351 77 39 0 7 83 32 108 2.13e-19 80.9
Msa0523500 AT2G21690.1 47.126 87 41 1 4 85 5 91 2.23e-19 78.2
Msa0523500 AT1G60650.2 42.683 82 47 0 4 85 10 91 4.53e-19 81.3
Msa0523500 AT1G60650.1 42.683 82 47 0 4 85 10 91 4.53e-19 81.3
Msa0523500 AT2G37510.1 51.351 74 36 0 7 80 35 108 3.65e-18 75.9
Msa0523500 AT2G46780.1 43.269 104 52 3 7 104 23 125 4.22e-18 79.3
Msa0523500 AT2G46780.2 43.269 104 52 3 7 104 23 125 4.22e-18 79.3
Msa0523500 AT5G19960.1 35.200 125 79 2 8 132 9 131 5.94e-18 79.0
Msa0523500 AT1G22910.1 44.681 94 46 2 7 95 14 106 8.67e-18 77.4
Msa0523500 AT1G22910.2 44.681 94 46 2 7 95 14 106 1.04e-17 77.0
Msa0523500 AT1G22330.1 41.176 102 51 2 7 100 18 118 1.70e-17 77.0
Msa0523500 AT4G13860.1 45.205 73 40 0 7 79 4 76 3.49e-17 71.6
Msa0523500 AT1G22910.3 44.681 94 46 2 7 95 14 106 6.12e-17 76.3
Msa0523500 AT1G60000.1 45.122 82 45 0 2 83 173 254 6.49e-17 75.1
Msa0523500 AT3G08000.1 44.048 84 47 0 7 90 42 125 7.20e-17 72.4
Msa0523500 AT5G59865.1 45.679 81 44 0 7 87 27 107 8.17e-17 71.6
Msa0523500 AT1G20880.2 46.053 76 40 1 7 82 25 99 2.14e-16 73.9
Msa0523500 AT1G20880.3 46.053 76 40 1 7 82 25 99 2.14e-16 73.9
Msa0523500 AT1G20880.1 46.053 76 40 1 7 82 25 99 2.14e-16 73.9
Msa0523500 AT3G07810.1 40.506 79 45 1 7 85 7 83 2.94e-16 74.7
Msa0523500 AT3G07810.2 40.506 79 45 1 7 85 7 83 2.94e-16 74.7
Msa0523500 AT1G76460.1 46.053 76 40 1 7 82 25 99 8.10e-16 72.4
Msa0523500 AT1G33470.3 46.753 77 38 2 7 82 8 82 9.21e-16 71.6
Msa0523500 AT1G33470.1 46.753 77 38 2 7 82 8 82 1.05e-15 71.6
Msa0523500 AT1G33470.2 46.753 77 38 2 7 82 8 82 1.11e-15 71.6
Msa0523500 AT3G46020.1 38.272 81 50 0 4 84 5 85 1.63e-15 67.8
Msa0523500 AT2G33410.1 32.143 112 74 1 7 118 7 116 2.52e-15 72.0
Msa0523500 AT1G78260.1 43.421 76 42 1 7 82 18 92 5.64e-15 70.1
Msa0523500 AT4G26650.2 37.975 79 47 1 7 85 13 89 5.90e-15 70.9
Msa0523500 AT5G55550.5 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa0523500 AT5G55550.5 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa0523500 AT5G55550.4 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa0523500 AT5G55550.4 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa0523500 AT5G55550.9 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa0523500 AT5G55550.9 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa0523500 AT5G55550.7 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa0523500 AT5G55550.7 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa0523500 AT5G55550.1 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa0523500 AT5G55550.1 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa0523500 AT4G26650.1 37.975 79 47 1 7 85 16 92 6.52e-15 70.9
Msa0523500 AT5G55550.2 39.241 79 46 1 7 85 7 83 7.01e-15 70.9
Msa0523500 AT5G55550.2 31.193 109 74 1 7 115 111 218 7.05e-12 62.0
Msa0523500 AT5G55550.3 39.241 79 46 1 7 85 7 83 7.01e-15 70.9
Msa0523500 AT5G55550.3 31.193 109 74 1 7 115 111 218 7.05e-12 62.0
Msa0523500 AT5G55550.6 39.241 79 46 1 7 85 7 83 7.01e-15 70.9
Msa0523500 AT5G55550.6 31.193 109 74 1 7 115 111 218 7.05e-12 62.0
Msa0523500 AT5G55550.11 39.241 79 46 1 7 85 7 83 7.01e-15 70.9
Msa0523500 AT5G55550.11 31.193 109 74 1 7 115 111 218 7.05e-12 62.0
Msa0523500 AT1G78260.2 43.421 76 42 1 7 82 18 92 8.93e-15 69.3
Msa0523500 AT4G14300.1 37.975 79 47 1 7 85 7 83 1.06e-14 70.1
Msa0523500 AT4G14300.2 37.975 79 47 1 7 85 7 83 1.06e-14 70.1
Msa0523500 AT1G73530.1 36.250 80 51 0 7 86 78 157 3.49e-14 66.6
Msa0523500 AT5G54580.1 44.048 84 47 0 2 85 52 135 5.17e-14 65.5
Msa0523500 AT2G27330.1 29.348 92 65 0 8 99 23 114 5.44e-14 64.3
Msa0523500 AT1G01080.1 41.463 82 45 2 1 81 207 286 6.47e-14 67.4
Msa0523500 AT1G01080.2 41.463 82 45 2 1 81 208 287 7.00e-14 67.4
Msa0523500 AT2G23350.1 48.611 72 36 1 8 79 136 206 1.04e-13 67.4
Msa0523500 AT5G55550.8 40.845 71 40 1 7 77 7 75 1.04e-13 67.4
Msa0523500 AT5G55550.8 31.193 109 74 1 7 115 158 265 6.32e-12 62.4
Msa0523500 AT5G55550.10 40.845 71 40 1 7 77 7 75 1.15e-13 67.4
Msa0523500 AT5G55550.10 31.193 109 74 1 7 115 158 265 6.17e-12 62.4
Msa0523500 AT5G53720.1 43.590 78 43 1 5 82 8 84 1.19e-13 63.2
Msa0523500 AT3G20930.1 37.500 80 50 0 7 86 283 362 1.25e-13 67.0
Msa0523500 AT3G13224.2 34.568 81 53 0 7 87 110 190 1.40e-13 66.6
Msa0523500 AT3G13224.2 34.940 83 52 1 7 89 20 100 4.19e-13 65.5
Msa0523500 AT3G13224.3 34.409 93 55 2 1 89 1 91 2.03e-13 66.2
Msa0523500 AT3G13224.3 34.568 81 53 0 7 87 101 181 2.75e-13 65.9
Msa0523500 AT3G54770.1 42.105 76 43 1 7 82 18 92 3.13e-13 65.1
Msa0523500 AT3G54770.3 42.105 76 43 1 7 82 18 92 3.19e-13 65.1
Msa0523500 AT5G47620.1 35.443 79 49 1 7 85 7 83 4.00e-13 65.5
Msa0523500 AT5G47620.2 35.443 79 49 1 7 85 7 83 4.00e-13 65.5
Msa0523500 AT5G47620.5 35.443 79 49 1 7 85 7 83 4.00e-13 65.5
Msa0523500 AT4G13860.2 47.458 59 31 0 22 80 2 60 4.82e-13 60.8
Msa0523500 AT3G52150.2 44.156 77 43 0 6 82 177 253 7.23e-13 63.9
Msa0523500 AT3G52150.1 44.156 77 43 0 6 82 177 253 7.23e-13 63.9
Msa0523500 AT1G17640.2 38.750 80 47 1 7 86 67 144 8.20e-13 64.7
Msa0523500 AT1G17640.2 32.990 97 64 1 7 103 158 253 9.59e-13 64.3
Msa0523500 AT1G17640.1 38.750 80 47 1 7 86 67 144 8.20e-13 64.7
Msa0523500 AT1G17640.1 32.990 97 64 1 7 103 158 253 9.59e-13 64.3
Msa0523500 AT1G17640.3 38.750 80 47 1 7 86 67 144 8.20e-13 64.7
Msa0523500 AT1G17640.3 32.990 97 64 1 7 103 158 253 9.59e-13 64.3
Msa0523500 AT2G37510.2 38.384 99 36 1 7 80 35 133 1.48e-12 62.0
Msa0523500 AT2G18510.1 40.964 83 48 1 8 89 114 196 1.59e-12 63.9
Msa0523500 AT5G53680.1 49.254 67 27 2 7 66 14 80 2.38e-12 61.2
Msa0523500 AT3G14100.1 41.026 78 46 0 5 82 143 220 2.60e-12 63.2
Msa0523500 AT5G51300.1 42.308 78 45 0 5 82 479 556 4.68e-12 62.8
Msa0523500 AT5G51300.2 42.308 78 45 0 5 82 479 556 4.68e-12 62.8
Msa0523500 AT5G51300.3 42.308 78 45 0 5 82 479 556 4.68e-12 62.8
Msa0523500 AT1G54080.1 41.026 78 46 0 5 82 147 224 5.37e-12 62.4
Msa0523500 AT5G47620.4 36.111 72 44 1 7 78 7 76 5.52e-12 62.4
Msa0523500 AT5G40490.1 32.530 83 56 0 7 89 131 213 8.36e-12 62.0
Msa0523500 AT3G13224.1 34.524 84 53 1 7 90 20 101 1.07e-11 60.5
Msa0523500 AT5G11412.1 42.105 76 43 1 7 82 53 127 1.10e-11 59.7
Msa0523500 AT2G22100.1 38.272 81 49 1 8 88 165 244 1.48e-11 61.2
Msa0523500 AT3G20930.2 35.294 85 50 1 7 86 283 367 1.64e-11 60.8
Msa0523500 AT2G19380.1 45.614 57 31 0 9 65 411 467 2.01e-11 60.8
Msa0523500 AT2G22090.2 39.080 87 51 2 7 93 105 189 2.64e-11 60.5
Msa0523500 AT2G22090.1 39.080 87 51 2 7 93 105 189 2.80e-11 60.1
Msa0523500 AT5G40490.2 32.530 83 56 0 7 89 114 196 3.98e-11 60.1
Msa0523500 AT1G01080.3 42.857 56 32 0 1 56 207 262 4.69e-11 59.3

Find 97 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCCACGTGCCATTAAATT+TGG 0.173275 3_4:+76863741 None:intergenic
TCAACTTCCGCCATGGAAAT+TGG 0.191164 3_4:+76864977 None:intergenic
CATTAAATTTGGACCATTAA+TGG 0.212323 3_4:+76863752 None:intergenic
ATCTATCTTGGTGCCATTAA+TGG 0.250089 3_4:-76863765 Msa0523500:three_prime_UTR
TAATGACACGGTGCCATTAA+TGG 0.253071 3_4:+76863836 None:intergenic
ATTCCAAATCTAAACCAATT+TGG 0.260003 3_4:+76864051 None:intergenic
CATTAATGGTCCAAATTTAA+TGG 0.279553 3_4:-76863751 Msa0523500:three_prime_UTR
TATCTGTTATTATGCCAAAT+TGG 0.291052 3_4:-76864065 Msa0523500:three_prime_UTR
ACTGGAAGATCAAGAGGATT+TGG 0.294131 3_4:-76864547 Msa0523500:CDS
ATGCCAAATTGGTTTAGATT+TGG 0.298687 3_4:-76864054 Msa0523500:three_prime_UTR
TAGCACTTTGGTGCCATTAA+TGG 0.302265 3_4:-76863849 Msa0523500:three_prime_UTR
CGCGGAGGTGGTGGTGGATA+TGG 0.305180 3_4:-76864385 Msa0523500:CDS
CGTCGTGGAGGTGGTGGATA+TGG 0.325761 3_4:-76864349 Msa0523500:CDS
CAACTTCCGCCATGGAAATT+GGG 0.336480 3_4:+76864978 None:intergenic
AAATAAACAGCATAAAGTTT+TGG 0.339669 3_4:+76863926 None:intergenic
AGTGGCGGAGGCGGTGGTGG+TGG 0.340361 3_4:-76864415 Msa0523500:CDS
GAAATTGAATGATCTGAATA+TGG 0.351510 3_4:-76864090 Msa0523500:three_prime_UTR
GTTATCACCGATCGTGAAAC+TGG 0.362798 3_4:-76864565 Msa0523500:CDS
ATCACCGCCTCTCGAGTATC+TGG 0.373006 3_4:+76864180 None:intergenic
GGCGGAGGCGGTGGTGGTGG+TGG 0.379371 3_4:-76864412 Msa0523500:CDS
GGCAGTGGCGGAGGCGGTGG+TGG 0.383736 3_4:-76864418 Msa0523500:CDS
CGTGGCAGTGGCGGAGGCGG+TGG 0.400483 3_4:-76864421 Msa0523500:CDS
GGATACCGCGGAGGTGGTGG+TGG 0.412147 3_4:-76864391 Msa0523500:CDS
AAACACATTGCCATTGCATC+TGG 0.416516 3_4:-76863799 Msa0523500:three_prime_UTR
GGTGGATACCGCGGAGGTGG+TGG 0.418043 3_4:-76864394 Msa0523500:CDS
CGCCATGGAAATTGGGGAAA+GGG 0.422744 3_4:+76864985 None:intergenic
TGTTCTTCATGTGGTTCTCC+TGG 0.437016 3_4:-76863999 Msa0523500:three_prime_UTR
GGTGGCGAACGTCGTGGAGG+TGG 0.444469 3_4:-76864358 Msa0523500:CDS
TAGATTTGGAATTGTGTTTG+TGG 0.450563 3_4:-76864040 Msa0523500:three_prime_UTR
GGTGGTGGATATGGCGGAGG+TGG 0.452527 3_4:-76864376 Msa0523500:CDS
CAGAGTCGTGGCAGTGGCGG+AGG 0.453358 3_4:-76864427 Msa0523500:CDS
GGTGGTGGATACCGCGGAGG+TGG 0.455995 3_4:-76864397 Msa0523500:CDS
AGTCGTGGCAGTGGCGGAGG+CGG 0.456959 3_4:-76864424 Msa0523500:CDS
GTGGCTCTGACAGTAGAAAC+TGG 0.459380 3_4:-76864153 Msa0523500:CDS
GTTGAATACCGATGCTTTGT+TGG 0.464495 3_4:-76864959 Msa0523500:CDS
CTTTGTTGGTGGACTTGCAT+GGG 0.465470 3_4:-76864945 Msa0523500:CDS
GGCGAACGTCGTGGAGGTGG+TGG 0.469494 3_4:-76864355 Msa0523500:CDS
GGATATGGTGGAGGTGGCTA+CGG 0.473053 3_4:-76864214 Msa0523500:intron
CTTAGTGAAGGGTAGCACTT+TGG 0.476115 3_4:-76863861 Msa0523500:three_prime_UTR
GTGTTTGTGGTTCTGAGTAA+TGG 0.478890 3_4:-76864027 Msa0523500:three_prime_UTR
GCCACGCAACACATCTATCT+TGG 0.480265 3_4:-76863777 Msa0523500:three_prime_UTR
TTCACTGCCGGGTAGCACTT+TGG 0.491165 3_4:+76863719 None:intergenic
AACCCTTTCCCCAATTTCCA+TGG 0.492490 3_4:-76864987 Msa0523500:exon
CTCAAGAGCTTCGTTGTCAG+TGG 0.495714 3_4:+76864922 None:intergenic
GGTGGTGGTGGATATGGCGG+AGG 0.496613 3_4:-76864379 Msa0523500:CDS
GCGATGAAAGATGCGATTGA+AGG 0.503882 3_4:-76864499 Msa0523500:CDS
ATGAACGGCCAAGATCTTGA+TGG 0.505893 3_4:-76864475 Msa0523500:CDS
GGTGGAGGTGGCTACGGAGA+TGG 0.509409 3_4:-76864208 Msa0523500:intron
TGTCACATTCTCTACGGAGC+AGG 0.509697 3_4:-76864521 Msa0523500:CDS
CGTGGAGGTGGTGGATATGG+TGG 0.512534 3_4:-76864346 Msa0523500:CDS
GCTTTGTTGGTGGACTTGCA+TGG 0.519425 3_4:-76864946 Msa0523500:CDS
TGGATTTGTCACATTCTCTA+CGG 0.520808 3_4:-76864527 Msa0523500:CDS
TGATCTTCCAGTTTCACGAT+CGG 0.521619 3_4:+76864558 None:intergenic
GAGGCTCAGAGTCGTGGCAG+TGG 0.522443 3_4:-76864433 Msa0523500:CDS
AATTGGGGAAAGGGTTAAAG+TGG 0.527355 3_4:+76864994 None:intergenic
TGTTACGTCCATCAAGATCT+TGG 0.527371 3_4:+76864467 None:intergenic
TCGAGAGGCGGTGATGGCGG+TGG 0.528930 3_4:-76864172 Msa0523500:CDS
AACTTCCGCCATGGAAATTG+GGG 0.531831 3_4:+76864979 None:intergenic
GGAGGTGGTGGATATGGTGG+AGG 0.534655 3_4:-76864343 Msa0523500:intron
CCGCCATGGAAATTGGGGAA+AGG 0.539529 3_4:+76864984 None:intergenic
CGATGAAAGATGCGATTGAA+GGG 0.542649 3_4:-76864498 Msa0523500:CDS
TGGTGAAATCGTCGAATCGA+AGG 0.542807 3_4:-76864882 Msa0523500:intron
GCTCAGAGTCGTGGCAGTGG+CGG 0.545233 3_4:-76864430 Msa0523500:CDS
CGATTCGACGATTTCACCAA+AGG 0.547099 3_4:+76864886 None:intergenic
TATTCATACTTACTTAGTGA+AGG 0.547125 3_4:-76863873 Msa0523500:three_prime_UTR
AGATACTCGAGAGGCGGTGA+TGG 0.555850 3_4:-76864178 Msa0523500:CDS
GGAGGTGGCTACGGAGATGG+TGG 0.556120 3_4:-76864205 Msa0523500:intron
ATGGTTTCGTGTTCTTCATG+TGG 0.561892 3_4:-76864008 Msa0523500:three_prime_UTR
CTTATAGTAGCAATAGAGTA+CGG 0.563266 3_4:+76863898 None:intergenic
CAGTTCATCAAATGAATAGT+CGG 0.565020 3_4:-76863959 Msa0523500:three_prime_UTR
CGTGAAACTGGAAGATCAAG+AGG 0.575579 3_4:-76864553 Msa0523500:CDS
GAATACCGATGCTTTGTTGG+TGG 0.577401 3_4:-76864956 Msa0523500:CDS
TACTCGAGAGGCGGTGATGG+CGG 0.579779 3_4:-76864175 Msa0523500:CDS
ATTCATACTTACTTAGTGAA+GGG 0.580210 3_4:-76863872 Msa0523500:three_prime_UTR
ACGTAACATCACTGTGAATG+AGG 0.581570 3_4:-76864452 Msa0523500:CDS
TCGGTATTCAACTTCCGCCA+TGG 0.595134 3_4:+76864970 None:intergenic
GGAGGTGGTGGTGGATATGG+CGG 0.597374 3_4:-76864382 Msa0523500:CDS
TGTTGCGTGGCCAGATGCAA+TGG 0.617822 3_4:+76863789 None:intergenic
ATTGGGGAAAGGGTTAAAGT+GGG 0.618625 3_4:+76864995 None:intergenic
CTCTGACAGTAGAAACTGGA+GGG 0.619176 3_4:-76864149 Msa0523500:CDS
GATGCGATTGAAGGGATGAA+CGG 0.619661 3_4:-76864490 Msa0523500:CDS
GTGAATGAGGCTCAGAGTCG+TGG 0.619893 3_4:-76864439 Msa0523500:CDS
GGTGGTGGTGGTGGATACCG+CGG 0.621729 3_4:-76864403 Msa0523500:CDS
GGCGGAGGTGGCGAACGTCG+TGG 0.626375 3_4:-76864364 Msa0523500:CDS
CAAGTCCACCAACAAAGCAT+CGG 0.636350 3_4:+76864951 None:intergenic
GGCTCCAGATACTCGAGAGG+CGG 0.636811 3_4:-76864184 Msa0523500:CDS
GGAGGTGGCGAACGTCGTGG+AGG 0.643779 3_4:-76864361 Msa0523500:CDS
GGTGGTGGTGGATACCGCGG+AGG 0.661432 3_4:-76864400 Msa0523500:CDS
GCAATGGCAATGTGTTTGTG+TGG 0.671659 3_4:+76863805 None:intergenic
CCTTTCCCCAATTTCCATGG+CGG 0.683414 3_4:-76864984 Msa0523500:exon
GGTCCAAATTTAATGGCACG+TGG 0.697603 3_4:-76863744 Msa0523500:three_prime_UTR
CATATCCACCACCACCTCCG+CGG 0.713479 3_4:+76864386 None:intergenic
GCTCTGACAGTAGAAACTGG+AGG 0.714944 3_4:-76864150 Msa0523500:CDS
GTTCATATGAACAAACAACC+AGG 0.717060 3_4:+76863981 None:intergenic
GGTGGCTCCAGATACTCGAG+AGG 0.728248 3_4:-76864187 Msa0523500:CDS
GTGGCTCAGATTTAATGACA+CGG 0.739511 3_4:+76863824 None:intergenic
ACCAAGATAGATGTGTTGCG+TGG 0.748458 3_4:+76863776 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTAATCACTTCAAAT+CGG - chr3_4:76864072-76864091 Msa0523500:three_prime_UTR 15.0%
!!! TTTTATTAATCACTTCAAAT+CGG - chr3_4:76864072-76864091 Msa0523500:three_prime_UTR 15.0%
!!! AAATAAACAGCATAAAGTTT+TGG + chr3_4:76864855-76864874 None:intergenic 20.0%
!!! AAATAAACAGCATAAAGTTT+TGG + chr3_4:76864855-76864874 None:intergenic 20.0%
!!! ACGATTCTTTGTTTTTGTTT+AGG - chr3_4:76864191-76864210 Msa0523500:CDS 25.0%
! GAAATTGAATGATCTGAATA+TGG - chr3_4:76864688-76864707 Msa0523500:intron 25.0%
! TATCTGTTATTATGCCAAAT+TGG - chr3_4:76864713-76864732 Msa0523500:intron 25.0%
! ATTCCAAATCTAAACCAATT+TGG + chr3_4:76864730-76864749 None:intergenic 25.0%
! TATTCATACTTACTTAGTGA+AGG - chr3_4:76864905-76864924 Msa0523500:CDS 25.0%
! ATTCATACTTACTTAGTGAA+GGG - chr3_4:76864906-76864925 Msa0523500:CDS 25.0%
! CATTAAATTTGGACCATTAA+TGG + chr3_4:76865029-76865048 None:intergenic 25.0%
! CATTAATGGTCCAAATTTAA+TGG - chr3_4:76865027-76865046 Msa0523500:five_prime_UTR 25.0%
!!! ACGATTCTTTGTTTTTGTTT+AGG - chr3_4:76864191-76864210 Msa0523500:CDS 25.0%
! GAAATTGAATGATCTGAATA+TGG - chr3_4:76864688-76864707 Msa0523500:intron 25.0%
! TATCTGTTATTATGCCAAAT+TGG - chr3_4:76864713-76864732 Msa0523500:intron 25.0%
! ATTCCAAATCTAAACCAATT+TGG + chr3_4:76864730-76864749 None:intergenic 25.0%
! TATTCATACTTACTTAGTGA+AGG - chr3_4:76864905-76864924 Msa0523500:CDS 25.0%
! ATTCATACTTACTTAGTGAA+GGG - chr3_4:76864906-76864925 Msa0523500:CDS 25.0%
! CATTAAATTTGGACCATTAA+TGG + chr3_4:76865029-76865048 None:intergenic 25.0%
! CATTAATGGTCCAAATTTAA+TGG - chr3_4:76865027-76865046 Msa0523500:five_prime_UTR 25.0%
TGTGTAAATTGAAGATCGAT+CGG - chr3_4:76864113-76864132 Msa0523500:three_prime_UTR 30.0%
! ATGCCAAATTGGTTTAGATT+TGG - chr3_4:76864724-76864743 Msa0523500:intron 30.0%
! TAGATTTGGAATTGTGTTTG+TGG - chr3_4:76864738-76864757 Msa0523500:intron 30.0%
CAGTTCATCAAATGAATAGT+CGG - chr3_4:76864819-76864838 Msa0523500:intron 30.0%
CTTATAGTAGCAATAGAGTA+CGG + chr3_4:76864883-76864902 None:intergenic 30.0%
TGTGTAAATTGAAGATCGAT+CGG - chr3_4:76864113-76864132 Msa0523500:three_prime_UTR 30.0%
! ATGCCAAATTGGTTTAGATT+TGG - chr3_4:76864724-76864743 Msa0523500:intron 30.0%
! TAGATTTGGAATTGTGTTTG+TGG - chr3_4:76864738-76864757 Msa0523500:intron 30.0%
CAGTTCATCAAATGAATAGT+CGG - chr3_4:76864819-76864838 Msa0523500:intron 30.0%
CTTATAGTAGCAATAGAGTA+CGG + chr3_4:76864883-76864902 None:intergenic 30.0%
GTGGGAAAACAAGAAAAAAG+AGG + chr3_4:76863768-76863787 None:intergenic 35.0%
TGGATTTGTCACATTCTCTA+CGG - chr3_4:76864251-76864270 Msa0523500:intron 35.0%
GTTCATATGAACAAACAACC+AGG + chr3_4:76864800-76864819 None:intergenic 35.0%
!! ATCTATCTTGGTGCCATTAA+TGG - chr3_4:76865013-76865032 Msa0523500:five_prime_UTR 35.0%
GTGGGAAAACAAGAAAAAAG+AGG + chr3_4:76863768-76863787 None:intergenic 35.0%
TGGATTTGTCACATTCTCTA+CGG - chr3_4:76864251-76864270 Msa0523500:intron 35.0%
GTTCATATGAACAAACAACC+AGG + chr3_4:76864800-76864819 None:intergenic 35.0%
!! ATCTATCTTGGTGCCATTAA+TGG - chr3_4:76865013-76865032 Msa0523500:five_prime_UTR 35.0%
ATTGGGGAAAGGGTTAAAGT+GGG + chr3_4:76863786-76863805 None:intergenic 40.0%
AATTGGGGAAAGGGTTAAAG+TGG + chr3_4:76863787-76863806 None:intergenic 40.0%
GTTGAATACCGATGCTTTGT+TGG - chr3_4:76863819-76863838 Msa0523500:three_prime_UTR 40.0%
!!! GAGAAAGCGTTTTCTTCCTT+TGG - chr3_4:76863876-76863895 Msa0523500:three_prime_UTR 40.0%
TTTCCGATCTCAGCAACAAA+CGG + chr3_4:76863923-76863942 None:intergenic 40.0%
ATCGGAAATCTGACACGATT+CGG - chr3_4:76863935-76863954 Msa0523500:three_prime_UTR 40.0%
! AATCTGACACGATTCGGATT+TGG - chr3_4:76863941-76863960 Msa0523500:three_prime_UTR 40.0%
ACAGAACAGAACAGAACAGA+AGG + chr3_4:76863980-76863999 None:intergenic 40.0%
TGATCTTCCAGTTTCACGAT+CGG + chr3_4:76864223-76864242 None:intergenic 40.0%
ACTGGAAGATCAAGAGGATT+TGG - chr3_4:76864231-76864250 Msa0523500:intron 40.0%
CGATGAAAGATGCGATTGAA+GGG - chr3_4:76864280-76864299 Msa0523500:intron 40.0%
TGTTACGTCCATCAAGATCT+TGG + chr3_4:76864314-76864333 None:intergenic 40.0%
ACGTAACATCACTGTGAATG+AGG - chr3_4:76864326-76864345 Msa0523500:intron 40.0%
!! GTGTTTGTGGTTCTGAGTAA+TGG - chr3_4:76864751-76864770 Msa0523500:intron 40.0%
ATGGTTTCGTGTTCTTCATG+TGG - chr3_4:76864770-76864789 Msa0523500:intron 40.0%
!! TAGCACTTTGGTGCCATTAA+TGG - chr3_4:76864929-76864948 Msa0523500:CDS 40.0%
!! TAATGACACGGTGCCATTAA+TGG + chr3_4:76864945-76864964 None:intergenic 40.0%
GTGGCTCAGATTTAATGACA+CGG + chr3_4:76864957-76864976 None:intergenic 40.0%
AAACACATTGCCATTGCATC+TGG - chr3_4:76864979-76864998 Msa0523500:exon 40.0%
ATTGGGGAAAGGGTTAAAGT+GGG + chr3_4:76863786-76863805 None:intergenic 40.0%
AATTGGGGAAAGGGTTAAAG+TGG + chr3_4:76863787-76863806 None:intergenic 40.0%
GTTGAATACCGATGCTTTGT+TGG - chr3_4:76863819-76863838 Msa0523500:three_prime_UTR 40.0%
!!! GAGAAAGCGTTTTCTTCCTT+TGG - chr3_4:76863876-76863895 Msa0523500:three_prime_UTR 40.0%
TTTCCGATCTCAGCAACAAA+CGG + chr3_4:76863923-76863942 None:intergenic 40.0%
ATCGGAAATCTGACACGATT+CGG - chr3_4:76863935-76863954 Msa0523500:three_prime_UTR 40.0%
! AATCTGACACGATTCGGATT+TGG - chr3_4:76863941-76863960 Msa0523500:three_prime_UTR 40.0%
ACAGAACAGAACAGAACAGA+AGG + chr3_4:76863980-76863999 None:intergenic 40.0%
TGATCTTCCAGTTTCACGAT+CGG + chr3_4:76864223-76864242 None:intergenic 40.0%
ACTGGAAGATCAAGAGGATT+TGG - chr3_4:76864231-76864250 Msa0523500:intron 40.0%
CGATGAAAGATGCGATTGAA+GGG - chr3_4:76864280-76864299 Msa0523500:intron 40.0%
TGTTACGTCCATCAAGATCT+TGG + chr3_4:76864314-76864333 None:intergenic 40.0%
ACGTAACATCACTGTGAATG+AGG - chr3_4:76864326-76864345 Msa0523500:intron 40.0%
!! GTGTTTGTGGTTCTGAGTAA+TGG - chr3_4:76864751-76864770 Msa0523500:intron 40.0%
ATGGTTTCGTGTTCTTCATG+TGG - chr3_4:76864770-76864789 Msa0523500:intron 40.0%
!! TAGCACTTTGGTGCCATTAA+TGG - chr3_4:76864929-76864948 Msa0523500:CDS 40.0%
!! TAATGACACGGTGCCATTAA+TGG + chr3_4:76864945-76864964 None:intergenic 40.0%
GTGGCTCAGATTTAATGACA+CGG + chr3_4:76864957-76864976 None:intergenic 40.0%
AAACACATTGCCATTGCATC+TGG - chr3_4:76864979-76864998 Msa0523500:exon 40.0%
AACCCTTTCCCCAATTTCCA+TGG - chr3_4:76863791-76863810 Msa0523500:three_prime_UTR 45.0%
AACTTCCGCCATGGAAATTG+GGG + chr3_4:76863802-76863821 None:intergenic 45.0%
CAACTTCCGCCATGGAAATT+GGG + chr3_4:76863803-76863822 None:intergenic 45.0%
TCAACTTCCGCCATGGAAAT+TGG + chr3_4:76863804-76863823 None:intergenic 45.0%
! GAATACCGATGCTTTGTTGG+TGG - chr3_4:76863822-76863841 Msa0523500:three_prime_UTR 45.0%
CAAGTCCACCAACAAAGCAT+CGG + chr3_4:76863830-76863849 None:intergenic 45.0%
!! CTTTGTTGGTGGACTTGCAT+GGG - chr3_4:76863833-76863852 Msa0523500:three_prime_UTR 45.0%
CGATTCGACGATTTCACCAA+AGG + chr3_4:76863895-76863914 None:intergenic 45.0%
TGGTGAAATCGTCGAATCGA+AGG - chr3_4:76863896-76863915 Msa0523500:three_prime_UTR 45.0%
GTTATCACCGATCGTGAAAC+TGG - chr3_4:76864213-76864232 Msa0523500:intron 45.0%
CGTGAAACTGGAAGATCAAG+AGG - chr3_4:76864225-76864244 Msa0523500:intron 45.0%
GCGATGAAAGATGCGATTGA+AGG - chr3_4:76864279-76864298 Msa0523500:intron 45.0%
GATGCGATTGAAGGGATGAA+CGG - chr3_4:76864288-76864307 Msa0523500:intron 45.0%
ATGAACGGCCAAGATCTTGA+TGG - chr3_4:76864303-76864322 Msa0523500:intron 45.0%
CTCTGACAGTAGAAACTGGA+GGG - chr3_4:76864629-76864648 Msa0523500:intron 45.0%
TGTTCTTCATGTGGTTCTCC+TGG - chr3_4:76864779-76864798 Msa0523500:intron 45.0%
CTTAGTGAAGGGTAGCACTT+TGG - chr3_4:76864917-76864936 Msa0523500:CDS 45.0%
GCAATGGCAATGTGTTTGTG+TGG + chr3_4:76864976-76864995 None:intergenic 45.0%
ACCAAGATAGATGTGTTGCG+TGG + chr3_4:76865005-76865024 None:intergenic 45.0%
GGTCCAAATTTAATGGCACG+TGG - chr3_4:76865034-76865053 Msa0523500:five_prime_UTR 45.0%
! GTGCCACGTGCCATTAAATT+TGG + chr3_4:76865040-76865059 None:intergenic 45.0%
AACCCTTTCCCCAATTTCCA+TGG - chr3_4:76863791-76863810 Msa0523500:three_prime_UTR 45.0%
AACTTCCGCCATGGAAATTG+GGG + chr3_4:76863802-76863821 None:intergenic 45.0%
CAACTTCCGCCATGGAAATT+GGG + chr3_4:76863803-76863822 None:intergenic 45.0%
TCAACTTCCGCCATGGAAAT+TGG + chr3_4:76863804-76863823 None:intergenic 45.0%
! GAATACCGATGCTTTGTTGG+TGG - chr3_4:76863822-76863841 Msa0523500:three_prime_UTR 45.0%
CAAGTCCACCAACAAAGCAT+CGG + chr3_4:76863830-76863849 None:intergenic 45.0%
!! CTTTGTTGGTGGACTTGCAT+GGG - chr3_4:76863833-76863852 Msa0523500:three_prime_UTR 45.0%
CGATTCGACGATTTCACCAA+AGG + chr3_4:76863895-76863914 None:intergenic 45.0%
TGGTGAAATCGTCGAATCGA+AGG - chr3_4:76863896-76863915 Msa0523500:three_prime_UTR 45.0%
GTTATCACCGATCGTGAAAC+TGG - chr3_4:76864213-76864232 Msa0523500:intron 45.0%
CGTGAAACTGGAAGATCAAG+AGG - chr3_4:76864225-76864244 Msa0523500:intron 45.0%
GCGATGAAAGATGCGATTGA+AGG - chr3_4:76864279-76864298 Msa0523500:intron 45.0%
GATGCGATTGAAGGGATGAA+CGG - chr3_4:76864288-76864307 Msa0523500:intron 45.0%
ATGAACGGCCAAGATCTTGA+TGG - chr3_4:76864303-76864322 Msa0523500:intron 45.0%
CTCTGACAGTAGAAACTGGA+GGG - chr3_4:76864629-76864648 Msa0523500:intron 45.0%
TGTTCTTCATGTGGTTCTCC+TGG - chr3_4:76864779-76864798 Msa0523500:intron 45.0%
CTTAGTGAAGGGTAGCACTT+TGG - chr3_4:76864917-76864936 Msa0523500:CDS 45.0%
GCAATGGCAATGTGTTTGTG+TGG + chr3_4:76864976-76864995 None:intergenic 45.0%
ACCAAGATAGATGTGTTGCG+TGG + chr3_4:76865005-76865024 None:intergenic 45.0%
GGTCCAAATTTAATGGCACG+TGG - chr3_4:76865034-76865053 Msa0523500:five_prime_UTR 45.0%
! GTGCCACGTGCCATTAAATT+TGG + chr3_4:76865040-76865059 None:intergenic 45.0%
CGCCATGGAAATTGGGGAAA+GGG + chr3_4:76863796-76863815 None:intergenic 50.0%
CCTTTCCCCAATTTCCATGG+CGG - chr3_4:76863794-76863813 Msa0523500:three_prime_UTR 50.0%
TCGGTATTCAACTTCCGCCA+TGG + chr3_4:76863811-76863830 None:intergenic 50.0%
!! GCTTTGTTGGTGGACTTGCA+TGG - chr3_4:76863832-76863851 Msa0523500:three_prime_UTR 50.0%
CTCAAGAGCTTCGTTGTCAG+TGG + chr3_4:76863859-76863878 None:intergenic 50.0%
! GGTCCGTTTGTTGCTGAGAT+CGG - chr3_4:76863917-76863936 Msa0523500:three_prime_UTR 50.0%
TGTCACATTCTCTACGGAGC+AGG - chr3_4:76864257-76864276 Msa0523500:intron 50.0%
!! GTGGCTCTGACAGTAGAAAC+TGG - chr3_4:76864625-76864644 Msa0523500:intron 50.0%
GCTCTGACAGTAGAAACTGG+AGG - chr3_4:76864628-76864647 Msa0523500:intron 50.0%
GCCACGCAACACATCTATCT+TGG - chr3_4:76865001-76865020 Msa0523500:five_prime_UTR 50.0%
CGCCATGGAAATTGGGGAAA+GGG + chr3_4:76863796-76863815 None:intergenic 50.0%
CCTTTCCCCAATTTCCATGG+CGG - chr3_4:76863794-76863813 Msa0523500:three_prime_UTR 50.0%
TCGGTATTCAACTTCCGCCA+TGG + chr3_4:76863811-76863830 None:intergenic 50.0%
!! GCTTTGTTGGTGGACTTGCA+TGG - chr3_4:76863832-76863851 Msa0523500:three_prime_UTR 50.0%
CTCAAGAGCTTCGTTGTCAG+TGG + chr3_4:76863859-76863878 None:intergenic 50.0%
! GGTCCGTTTGTTGCTGAGAT+CGG - chr3_4:76863917-76863936 Msa0523500:three_prime_UTR 50.0%
TGTCACATTCTCTACGGAGC+AGG - chr3_4:76864257-76864276 Msa0523500:intron 50.0%
!! GTGGCTCTGACAGTAGAAAC+TGG - chr3_4:76864625-76864644 Msa0523500:intron 50.0%
GCTCTGACAGTAGAAACTGG+AGG - chr3_4:76864628-76864647 Msa0523500:intron 50.0%
GCCACGCAACACATCTATCT+TGG - chr3_4:76865001-76865020 Msa0523500:five_prime_UTR 50.0%
CCGCCATGGAAATTGGGGAA+AGG + chr3_4:76863797-76863816 None:intergenic 55.0%
GTGAATGAGGCTCAGAGTCG+TGG - chr3_4:76864339-76864358 Msa0523500:intron 55.0%
!! CGTGATGGTGGTGGTGGATA+TGG - chr3_4:76864549-76864568 Msa0523500:CDS 55.0%
!! GATGGTGGTGGTGGATATGG+TGG - chr3_4:76864552-76864571 Msa0523500:CDS 55.0%
! GGATATGGTGGAGGTGGCTA+CGG - chr3_4:76864564-76864583 Msa0523500:CDS 55.0%
ATCACCGCCTCTCGAGTATC+TGG + chr3_4:76864601-76864620 None:intergenic 55.0%
AGATACTCGAGAGGCGGTGA+TGG - chr3_4:76864600-76864619 Msa0523500:intron 55.0%
TGTTGCGTGGCCAGATGCAA+TGG + chr3_4:76864992-76865011 None:intergenic 55.0%
CCGCCATGGAAATTGGGGAA+AGG + chr3_4:76863797-76863816 None:intergenic 55.0%
GTGAATGAGGCTCAGAGTCG+TGG - chr3_4:76864339-76864358 Msa0523500:intron 55.0%
!! CGTGATGGTGGTGGTGGATA+TGG - chr3_4:76864549-76864568 Msa0523500:CDS 55.0%
!! GATGGTGGTGGTGGATATGG+TGG - chr3_4:76864552-76864571 Msa0523500:CDS 55.0%
! GGATATGGTGGAGGTGGCTA+CGG - chr3_4:76864564-76864583 Msa0523500:CDS 55.0%
ATCACCGCCTCTCGAGTATC+TGG + chr3_4:76864601-76864620 None:intergenic 55.0%
AGATACTCGAGAGGCGGTGA+TGG - chr3_4:76864600-76864619 Msa0523500:intron 55.0%
TGTTGCGTGGCCAGATGCAA+TGG + chr3_4:76864992-76865011 None:intergenic 55.0%
CATATCCACCACCACCTCCG+CGG + chr3_4:76864395-76864414 None:intergenic 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG - chr3_4:76864396-76864415 Msa0523500:CDS 60.0%
!! CGTCGTGGAGGTGGTGGATA+TGG - chr3_4:76864429-76864448 Msa0523500:CDS 60.0%
!! CGTGGAGGTGGTGGATATGG+TGG - chr3_4:76864432-76864451 Msa0523500:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG - chr3_4:76864435-76864454 Msa0523500:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG - chr3_4:76864438-76864457 Msa0523500:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+CGG - chr3_4:76864441-76864460 Msa0523500:CDS 60.0%
! GTGGATATGGTGGAGGTGGC+GGG - chr3_4:76864442-76864461 Msa0523500:CDS 60.0%
! TGGATATGGTGGAGGTGGCG+GGG - chr3_4:76864443-76864462 Msa0523500:CDS 60.0%
TATGGAGGTGGTGACCGTCG+TGG - chr3_4:76864468-76864487 Msa0523500:CDS 60.0%
ATCCACCACCTCCTCCACGA+CGG + chr3_4:76864485-76864504 None:intergenic 60.0%
!! CGTGGAGGAGGTGGTGGATA+TGG - chr3_4:76864486-76864505 Msa0523500:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG - chr3_4:76864489-76864508 Msa0523500:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG - chr3_4:76864435-76864454 Msa0523500:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG - chr3_4:76864438-76864457 Msa0523500:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+CGG - chr3_4:76864498-76864517 Msa0523500:CDS 60.0%
! GTGGATATGGTGGAGGAGGC+GGG - chr3_4:76864499-76864518 Msa0523500:CDS 60.0%
! TGGATATGGTGGAGGAGGCG+GGG - chr3_4:76864500-76864519 Msa0523500:CDS 60.0%
!! GGTGGTGGTGGATATGGTGG+AGG - chr3_4:76864555-76864574 Msa0523500:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG - chr3_4:76864438-76864457 Msa0523500:CDS 60.0%
GGTGGCTCCAGATACTCGAG+AGG - chr3_4:76864591-76864610 Msa0523500:intron 60.0%
GGCTCCAGATACTCGAGAGG+CGG - chr3_4:76864594-76864613 Msa0523500:intron 60.0%
! TACTCGAGAGGCGGTGATGG+CGG - chr3_4:76864603-76864622 Msa0523500:intron 60.0%
CATATCCACCACCACCTCCG+CGG + chr3_4:76864395-76864414 None:intergenic 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG - chr3_4:76864396-76864415 Msa0523500:CDS 60.0%
!! CGTCGTGGAGGTGGTGGATA+TGG - chr3_4:76864429-76864448 Msa0523500:CDS 60.0%
!! CGTGGAGGTGGTGGATATGG+TGG - chr3_4:76864432-76864451 Msa0523500:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG - chr3_4:76864435-76864454 Msa0523500:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG - chr3_4:76864438-76864457 Msa0523500:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+CGG - chr3_4:76864441-76864460 Msa0523500:CDS 60.0%
! GTGGATATGGTGGAGGTGGC+GGG - chr3_4:76864442-76864461 Msa0523500:CDS 60.0%
! TGGATATGGTGGAGGTGGCG+GGG - chr3_4:76864443-76864462 Msa0523500:CDS 60.0%
TATGGAGGTGGTGACCGTCG+TGG - chr3_4:76864468-76864487 Msa0523500:CDS 60.0%
ATCCACCACCTCCTCCACGA+CGG + chr3_4:76864485-76864504 None:intergenic 60.0%
!! CGTGGAGGAGGTGGTGGATA+TGG - chr3_4:76864486-76864505 Msa0523500:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG - chr3_4:76864489-76864508 Msa0523500:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG - chr3_4:76864435-76864454 Msa0523500:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG - chr3_4:76864438-76864457 Msa0523500:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+CGG - chr3_4:76864498-76864517 Msa0523500:CDS 60.0%
! GTGGATATGGTGGAGGAGGC+GGG - chr3_4:76864499-76864518 Msa0523500:CDS 60.0%
! TGGATATGGTGGAGGAGGCG+GGG - chr3_4:76864500-76864519 Msa0523500:CDS 60.0%
!! GGTGGTGGTGGATATGGTGG+AGG - chr3_4:76864555-76864574 Msa0523500:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG - chr3_4:76864438-76864457 Msa0523500:CDS 60.0%
GGTGGCTCCAGATACTCGAG+AGG - chr3_4:76864591-76864610 Msa0523500:intron 60.0%
GGCTCCAGATACTCGAGAGG+CGG - chr3_4:76864594-76864613 Msa0523500:intron 60.0%
! TACTCGAGAGGCGGTGATGG+CGG - chr3_4:76864603-76864622 Msa0523500:intron 60.0%
GAGGCTCAGAGTCGTGGCAG+TGG - chr3_4:76864345-76864364 Msa0523500:CDS 65.0%
GCTCAGAGTCGTGGCAGTGG+CGG - chr3_4:76864348-76864367 Msa0523500:CDS 65.0%
!! GGTGGTGGTGGTGGATACCG+CGG - chr3_4:76864375-76864394 Msa0523500:CDS 65.0%
!! CGCGGAGGTGGTGGTGGATA+TGG - chr3_4:76864393-76864412 Msa0523500:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG - chr3_4:76864399-76864418 Msa0523500:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG - chr3_4:76864402-76864421 Msa0523500:CDS 65.0%
! GGATATGGTGGAGGTGGCGG+GGG - chr3_4:76864444-76864463 Msa0523500:CDS 65.0%
! GGATATGGTGGAGGAGGCGG+GGG - chr3_4:76864501-76864520 Msa0523500:CDS 65.0%
!! GGTTACGGAGGTGGTGGTGG+CGG - chr3_4:76864522-76864541 Msa0523500:CDS 65.0%
!! GGACGTCGTGATGGTGGTGG+TGG - chr3_4:76864543-76864562 Msa0523500:CDS 65.0%
GGTGGAGGTGGCTACGGAGA+TGG - chr3_4:76864570-76864589 Msa0523500:intron 65.0%
GGAGGTGGCTACGGAGATGG+TGG - chr3_4:76864573-76864592 Msa0523500:intron 65.0%
GAGGCTCAGAGTCGTGGCAG+TGG - chr3_4:76864345-76864364 Msa0523500:CDS 65.0%
GCTCAGAGTCGTGGCAGTGG+CGG - chr3_4:76864348-76864367 Msa0523500:CDS 65.0%
!! GGTGGTGGTGGTGGATACCG+CGG - chr3_4:76864375-76864394 Msa0523500:CDS 65.0%
!! CGCGGAGGTGGTGGTGGATA+TGG - chr3_4:76864393-76864412 Msa0523500:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG - chr3_4:76864399-76864418 Msa0523500:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG - chr3_4:76864402-76864421 Msa0523500:CDS 65.0%
! GGATATGGTGGAGGTGGCGG+GGG - chr3_4:76864444-76864463 Msa0523500:CDS 65.0%
! GGATATGGTGGAGGAGGCGG+GGG - chr3_4:76864501-76864520 Msa0523500:CDS 65.0%
!! GGTTACGGAGGTGGTGGTGG+CGG - chr3_4:76864522-76864541 Msa0523500:CDS 65.0%
!! GGACGTCGTGATGGTGGTGG+TGG - chr3_4:76864543-76864562 Msa0523500:CDS 65.0%
GGTGGAGGTGGCTACGGAGA+TGG - chr3_4:76864570-76864589 Msa0523500:intron 65.0%
GGAGGTGGCTACGGAGATGG+TGG - chr3_4:76864573-76864592 Msa0523500:intron 65.0%
CAGAGTCGTGGCAGTGGCGG+AGG - chr3_4:76864351-76864370 Msa0523500:CDS 70.0%
! AGTCGTGGCAGTGGCGGAGG+CGG - chr3_4:76864354-76864373 Msa0523500:CDS 70.0%
!! GGTGGTGGTGGATACCGCGG+AGG - chr3_4:76864378-76864397 Msa0523500:CDS 70.0%
!! GGTGGTGGATACCGCGGAGG+TGG - chr3_4:76864381-76864400 Msa0523500:CDS 70.0%
GGTGGATACCGCGGAGGTGG+TGG - chr3_4:76864384-76864403 Msa0523500:CDS 70.0%
! GGATACCGCGGAGGTGGTGG+TGG - chr3_4:76864387-76864406 Msa0523500:CDS 70.0%
GGAGGTGGCGAACGTCGTGG+AGG - chr3_4:76864417-76864436 Msa0523500:CDS 70.0%
GGTGGCGAACGTCGTGGAGG+TGG - chr3_4:76864420-76864439 Msa0523500:CDS 70.0%
GGCGAACGTCGTGGAGGTGG+TGG - chr3_4:76864423-76864442 Msa0523500:CDS 70.0%
!! GGTGGAGGTGGCGGGGGTTA+TGG - chr3_4:76864450-76864469 Msa0523500:CDS 70.0%
!! GGAGGTGGCGGGGGTTATGG+AGG - chr3_4:76864453-76864472 Msa0523500:CDS 70.0%
!! GGTGGCGGGGGTTATGGAGG+TGG - chr3_4:76864456-76864475 Msa0523500:CDS 70.0%
GGAGGTGGTGACCGTCGTGG+AGG - chr3_4:76864471-76864490 Msa0523500:CDS 70.0%
GGTGGTGACCGTCGTGGAGG+AGG - chr3_4:76864474-76864493 Msa0523500:CDS 70.0%
GGTGACCGTCGTGGAGGAGG+TGG - chr3_4:76864477-76864496 Msa0523500:CDS 70.0%
GACCGTCGTGGAGGAGGTGG+TGG - chr3_4:76864480-76864499 Msa0523500:CDS 70.0%
GGTGGAGGAGGCGGGGGTTA+CGG - chr3_4:76864507-76864526 Msa0523500:CDS 70.0%
! GGGGGTTACGGAGGTGGTGG+TGG - chr3_4:76864519-76864538 Msa0523500:CDS 70.0%
!! GGTGGTGGCGGACGTCGTGA+TGG - chr3_4:76864534-76864553 Msa0523500:CDS 70.0%
!! GGTGGCGGACGTCGTGATGG+TGG - chr3_4:76864537-76864556 Msa0523500:CDS 70.0%
!! GGCGGACGTCGTGATGGTGG+TGG - chr3_4:76864540-76864559 Msa0523500:CDS 70.0%
!! TCGAGAGGCGGTGATGGCGG+TGG - chr3_4:76864606-76864625 Msa0523500:intron 70.0%
CAGAGTCGTGGCAGTGGCGG+AGG - chr3_4:76864351-76864370 Msa0523500:CDS 70.0%
! AGTCGTGGCAGTGGCGGAGG+CGG - chr3_4:76864354-76864373 Msa0523500:CDS 70.0%
!! GGTGGTGGTGGATACCGCGG+AGG - chr3_4:76864378-76864397 Msa0523500:CDS 70.0%
!! GGTGGTGGATACCGCGGAGG+TGG - chr3_4:76864381-76864400 Msa0523500:CDS 70.0%
GGTGGATACCGCGGAGGTGG+TGG - chr3_4:76864384-76864403 Msa0523500:CDS 70.0%
! GGATACCGCGGAGGTGGTGG+TGG - chr3_4:76864387-76864406 Msa0523500:CDS 70.0%
GGAGGTGGCGAACGTCGTGG+AGG - chr3_4:76864417-76864436 Msa0523500:CDS 70.0%
GGTGGCGAACGTCGTGGAGG+TGG - chr3_4:76864420-76864439 Msa0523500:CDS 70.0%
GGCGAACGTCGTGGAGGTGG+TGG - chr3_4:76864423-76864442 Msa0523500:CDS 70.0%
!! GGTGGAGGTGGCGGGGGTTA+TGG - chr3_4:76864450-76864469 Msa0523500:CDS 70.0%
!! GGAGGTGGCGGGGGTTATGG+AGG - chr3_4:76864453-76864472 Msa0523500:CDS 70.0%
!! GGTGGCGGGGGTTATGGAGG+TGG - chr3_4:76864456-76864475 Msa0523500:CDS 70.0%
GGAGGTGGTGACCGTCGTGG+AGG - chr3_4:76864471-76864490 Msa0523500:CDS 70.0%
GGTGGTGACCGTCGTGGAGG+AGG - chr3_4:76864474-76864493 Msa0523500:CDS 70.0%
GGTGACCGTCGTGGAGGAGG+TGG - chr3_4:76864477-76864496 Msa0523500:CDS 70.0%
GACCGTCGTGGAGGAGGTGG+TGG - chr3_4:76864480-76864499 Msa0523500:CDS 70.0%
GGTGGAGGAGGCGGGGGTTA+CGG - chr3_4:76864507-76864526 Msa0523500:CDS 70.0%
! GGGGGTTACGGAGGTGGTGG+TGG - chr3_4:76864519-76864538 Msa0523500:CDS 70.0%
!! GGTGGTGGCGGACGTCGTGA+TGG - chr3_4:76864534-76864553 Msa0523500:CDS 70.0%
!! GGTGGCGGACGTCGTGATGG+TGG - chr3_4:76864537-76864556 Msa0523500:CDS 70.0%
!! GGCGGACGTCGTGATGGTGG+TGG - chr3_4:76864540-76864559 Msa0523500:CDS 70.0%
!! TCGAGAGGCGGTGATGGCGG+TGG - chr3_4:76864606-76864625 Msa0523500:intron 70.0%
! AGTGGCGGAGGCGGTGGTGG+TGG - chr3_4:76864363-76864382 Msa0523500:CDS 75.0%
GGCGGAGGTGGCGAACGTCG+TGG - chr3_4:76864414-76864433 Msa0523500:CDS 75.0%
GGAGGAGGCGGGGGTTACGG+AGG - chr3_4:76864510-76864529 Msa0523500:CDS 75.0%
GGAGGCGGGGGTTACGGAGG+TGG - chr3_4:76864513-76864532 Msa0523500:CDS 75.0%
GGCGGGGGTTACGGAGGTGG+TGG - chr3_4:76864516-76864535 Msa0523500:CDS 75.0%
! AGTGGCGGAGGCGGTGGTGG+TGG - chr3_4:76864363-76864382 Msa0523500:CDS 75.0%
GGCGGAGGTGGCGAACGTCG+TGG - chr3_4:76864414-76864433 Msa0523500:CDS 75.0%
GGAGGAGGCGGGGGTTACGG+AGG - chr3_4:76864510-76864529 Msa0523500:CDS 75.0%
GGAGGCGGGGGTTACGGAGG+TGG - chr3_4:76864513-76864532 Msa0523500:CDS 75.0%
GGCGGGGGTTACGGAGGTGG+TGG - chr3_4:76864516-76864535 Msa0523500:CDS 75.0%
!! CGTGGCAGTGGCGGAGGCGG+TGG - chr3_4:76864357-76864376 Msa0523500:CDS 80.0%
!! GGCAGTGGCGGAGGCGGTGG+TGG - chr3_4:76864360-76864379 Msa0523500:CDS 80.0%
!!! GGCGGAGGCGGTGGTGGTGG+TGG - chr3_4:76864366-76864385 Msa0523500:CDS 80.0%
!! CGTGGCAGTGGCGGAGGCGG+TGG - chr3_4:76864357-76864376 Msa0523500:CDS 80.0%
!! GGCAGTGGCGGAGGCGGTGG+TGG - chr3_4:76864360-76864379 Msa0523500:CDS 80.0%
!!! GGCGGAGGCGGTGGTGGTGG+TGG - chr3_4:76864366-76864385 Msa0523500:CDS 80.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 76863733 76865067 76863733 ID=Msa0523500;Name=Msa0523500
chr3_4 mRNA 76863733 76865067 76863733 ID=Msa0523500-mRNA-1;Parent=Msa0523500;Name=Msa0523500-mRNA-1;_AED=0.12;_eAED=0.12;_QI=77|1|1|1|0.5|0.33|3|411|143
chr3_4 exon 76863733 76864223 76863733 ID=Msa0523500-mRNA-1:exon:22422;Parent=Msa0523500-mRNA-1
chr3_4 exon 76864344 76864587 76864344 ID=Msa0523500-mRNA-1:exon:22421;Parent=Msa0523500-mRNA-1
chr3_4 exon 76864883 76865067 76864883 ID=Msa0523500-mRNA-1:exon:22420;Parent=Msa0523500-mRNA-1
chr3_4 five_prime_UTR 76864991 76865067 76864991 ID=Msa0523500-mRNA-1:five_prime_utr;Parent=Msa0523500-mRNA-1
chr3_4 CDS 76864883 76864990 76864883 ID=Msa0523500-mRNA-1:cds;Parent=Msa0523500-mRNA-1
chr3_4 CDS 76864344 76864587 76864344 ID=Msa0523500-mRNA-1:cds;Parent=Msa0523500-mRNA-1
chr3_4 CDS 76864144 76864223 76864144 ID=Msa0523500-mRNA-1:cds;Parent=Msa0523500-mRNA-1
chr3_4 three_prime_UTR 76863733 76864143 76863733 ID=Msa0523500-mRNA-1:three_prime_utr;Parent=Msa0523500-mRNA-1
Gene Sequence

>Msa0523500

ATGGCGGAAGTTGAATACCGATGCTTTGTTGGTGGACTTGCATGGGCCACTGACAACGAAGCTCTTGAGAAAGCGTTTTCTTCCTTTGGTGAAATCGTCGAATCGAAGGTTATCACCGATCGTGAAACTGGAAGATCAAGAGGATTTGGATTTGTCACATTCTCTACGGAGCAGGCGATGAAAGATGCGATTGAAGGGATGAACGGCCAAGATCTTGATGGACGTAACATCACTGTGAATGAGGCTCAGAGTCGTGGCAGTGGCGGAGGCGGTGGTGGTGGTGGATACCGCGGAGGTGGTGGTGGATATGGCGGAGGTGGCGAACGTCGTGGAGGTGGTGGATATGGTGGAGGTGGCTACGGAGATGGTGGCTCCAGATACTCGAGAGGCGGTGATGGCGGTGGCTCTGACAGTAGAAACTGGAGGGAATAA
Protein sequence

>Msa0523500

MAEVEYRCFVGGLAWATDNEALEKAFSSFGEIVESKVITDRETGRSRGFGFVTFSTEQAMKDAIEGMNGQDLDGRNITVNEAQSRGSGGGGGGGGYRGGGGGYGGGGERRGGGGYGGGGYGDGGSRYSRGGDGGGSDSRNWRE*