AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1418070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1418070 MtrunA17_Chr4g0035001 84.337 83 13 0 1 83 1 83 2.06e-48 153
Msa1418070 MtrunA17_Chr4g0035051 82.927 82 14 0 2 83 4 85 9.27e-48 150
Msa1418070 MtrunA17_Chr4g0035031 80.723 83 16 0 2 84 4 86 6.66e-45 145
Msa1418070 MtrunA17_Chr2g0278661 60.241 83 33 0 5 87 6 88 9.03e-33 115
Msa1418070 MtrunA17_Chr4g0050781 59.036 83 34 0 3 85 4 86 6.60e-32 112
Msa1418070 MtrunA17_Chr3g0121631 53.165 79 36 1 7 85 37 114 8.09e-25 92.8
Msa1418070 MtrunA17_Chr3g0116631 52.273 88 42 0 3 90 203 290 3.29e-24 94.7
Msa1418070 MtrunA17_Chr3g0116631 41.975 81 47 0 7 87 110 190 1.92e-13 65.5
Msa1418070 MtrunA17_Chr4g0061451 48.101 79 41 0 7 85 43 121 1.53e-23 92.4
Msa1418070 MtrunA17_Chr7g0219021 44.578 83 46 0 7 89 41 123 3.70e-23 91.3
Msa1418070 MtrunA17_Chr1g0210241 49.383 81 41 0 5 85 6 86 4.59e-23 91.3
Msa1418070 MtrunA17_Chr3g0086861 46.835 79 42 0 7 85 45 123 1.25e-22 92.0
Msa1418070 MtrunA17_Chr3g0119311 46.341 82 44 0 7 88 37 118 2.59e-22 85.9
Msa1418070 MtrunA17_Chr4g0058151 51.852 81 39 0 6 86 216 296 5.29e-22 89.0
Msa1418070 MtrunA17_Chr4g0058151 38.235 102 63 0 5 106 121 222 6.92e-17 75.1
Msa1418070 MtrunA17_Chr1g0181111 55.000 80 36 0 7 86 197 276 6.62e-22 88.2
Msa1418070 MtrunA17_Chr1g0181111 35.878 131 77 2 7 137 85 208 1.24e-11 60.5
Msa1418070 MtrunA17_Chr7g0263001 49.367 79 40 0 7 85 36 114 8.20e-20 79.7
Msa1418070 MtrunA17_Chr7g0266751 43.210 81 46 0 4 84 30 110 3.38e-19 78.2
Msa1418070 MtrunA17_Chr4g0017401 45.263 95 44 3 7 95 17 109 1.00e-18 79.7
Msa1418070 MtrunA17_Chr6g0481071 43.750 80 45 0 7 86 96 175 9.09e-18 75.5
Msa1418070 MtrunA17_Chr1g0168601 43.902 82 46 0 2 83 149 230 1.03e-17 76.3
Msa1418070 MtrunA17_Chr4g0042161 43.820 89 50 0 1 89 46 134 3.48e-17 73.2
Msa1418070 MtrunA17_Chr8g0346011 33.077 130 71 2 7 121 38 166 7.16e-17 75.1
Msa1418070 MtrunA17_Chr3g0144311 46.053 76 40 1 7 82 30 104 1.27e-16 74.3
Msa1418070 MtrunA17_Chr3g0127751 32.117 137 79 3 7 132 27 160 1.29e-16 73.9
Msa1418070 MtrunA17_Chr3g0101061 39.241 79 46 1 7 85 41 117 4.33e-16 73.9
Msa1418070 MtrunA17_Chr2g0327921 39.024 82 50 0 7 88 8 89 4.46e-16 68.6
Msa1418070 MtrunA17_Chr7g0219031 42.424 66 38 0 7 72 41 106 1.21e-15 68.2
Msa1418070 MtrunA17_Chr5g0447761 39.241 79 46 2 7 85 7 83 1.40e-14 69.3
Msa1418070 MtrunA17_Chr1g0198431 37.500 88 55 0 7 94 193 280 1.61e-14 68.6
Msa1418070 MtrunA17_Chr3g0110211 43.373 83 46 1 8 89 114 196 2.05e-14 68.9
Msa1418070 MtrunA17_Chr5g0426311 42.105 76 43 1 7 82 17 91 2.25e-14 68.2
Msa1418070 MtrunA17_Chr2g0290191 36.709 79 48 1 7 85 5 81 2.67e-14 68.6
Msa1418070 MtrunA17_Chr1g0186351 35.443 79 49 1 7 85 7 83 2.78e-14 68.6
Msa1418070 MtrunA17_Chr1g0186351 35.294 85 54 1 7 91 113 196 2.65e-13 65.9
Msa1418070 MtrunA17_Chr3g0088511 40.541 74 44 0 7 80 33 106 4.80e-14 64.7
Msa1418070 MtrunA17_Chr3g0120721 33.582 134 80 3 7 134 107 237 5.95e-14 67.4
Msa1418070 MtrunA17_Chr4g0031091 41.096 73 43 0 7 79 34 106 7.51e-14 63.5
Msa1418070 MtrunA17_Chr7g0276531 47.458 59 31 0 7 65 19 77 9.19e-14 66.2
Msa1418070 MtrunA17_Chr4g0065741 35.165 91 58 1 7 97 72 161 8.61e-13 62.0
Msa1418070 MtrunA17_Chr6g0449481 30.392 102 70 1 7 107 134 235 9.45e-13 63.9
Msa1418070 MtrunA17_Chr7g0267571 36.047 86 55 0 6 91 212 297 1.55e-12 63.2
Msa1418070 MtrunA17_Chr4g0038111 36.458 96 59 2 9 104 342 435 2.50e-12 63.2
Msa1418070 MtrunA17_Chr5g0419351 39.535 86 52 0 8 93 12 97 8.32e-12 59.7
Msa1418070 MtrunA17_Chr4g0062931 33.600 125 81 2 8 132 9 131 8.94e-12 61.6
Msa1418070 MtrunA17_Chr7g0265341 43.836 73 40 1 9 81 161 232 2.00e-11 60.5
Msa1418070 MtrunA17_Chr4g0044541 42.857 84 44 2 9 89 125 207 2.65e-11 60.1
Msa1418070 MtrunA17_Chr8g0360551 38.554 83 49 2 7 89 74 154 4.23e-11 59.3
Msa1418070 MtrunA17_Chr8g0344491 38.824 85 51 1 5 89 146 229 4.57e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1418070 AT4G39260.3 74.725 91 23 0 1 91 1 91 1.80e-46 146
Msa1418070 AT2G21660.1 79.012 81 17 0 3 83 5 85 2.55e-43 141
Msa1418070 AT2G21660.2 77.083 96 22 0 3 98 5 100 7.82e-42 136
Msa1418070 AT4G39260.1 72.289 83 23 0 1 83 1 83 1.64e-40 134
Msa1418070 AT4G39260.2 73.469 98 24 1 1 98 1 96 5.99e-38 125
Msa1418070 AT3G26420.1 53.465 101 47 0 3 103 4 104 5.70e-33 116
Msa1418070 AT4G39260.4 70.588 68 20 0 1 68 1 68 3.48e-32 110
Msa1418070 AT3G23830.2 57.317 82 35 0 7 88 36 117 3.06e-28 101
Msa1418070 AT3G23830.1 57.317 82 35 0 7 88 36 117 3.06e-28 101
Msa1418070 AT4G13850.3 54.878 82 37 0 7 88 36 117 5.01e-26 96.3
Msa1418070 AT4G13850.4 55.556 81 36 0 7 87 36 116 5.15e-26 95.5
Msa1418070 AT4G13850.2 54.878 82 37 0 7 88 36 117 6.35e-26 96.3
Msa1418070 AT4G13850.1 54.878 82 37 0 7 88 36 117 7.24e-26 96.3
Msa1418070 AT3G53460.1 51.807 83 40 0 7 89 258 340 1.60e-23 94.0
Msa1418070 AT3G53460.1 42.105 76 44 0 5 80 98 173 5.86e-11 59.3
Msa1418070 AT3G53460.3 51.807 83 40 0 7 89 258 340 1.60e-23 94.0
Msa1418070 AT3G53460.3 42.105 76 44 0 5 80 98 173 5.86e-11 59.3
Msa1418070 AT3G53460.2 51.807 83 40 0 7 89 258 340 1.60e-23 94.0
Msa1418070 AT3G53460.2 42.105 76 44 0 5 80 98 173 5.86e-11 59.3
Msa1418070 AT2G37220.1 50.617 81 40 0 7 87 205 285 1.99e-23 92.8
Msa1418070 AT2G37220.1 43.421 76 43 0 5 80 90 165 5.45e-13 64.7
Msa1418070 AT3G53460.4 51.807 83 40 0 7 89 279 361 2.28e-23 94.0
Msa1418070 AT4G24770.1 50.588 85 42 0 3 87 241 325 9.41e-23 91.7
Msa1418070 AT4G24770.1 40.196 102 61 0 5 106 149 250 1.44e-16 75.1
Msa1418070 AT4G24770.2 50.588 85 42 0 3 87 241 325 9.41e-23 91.7
Msa1418070 AT4G24770.2 40.196 102 61 0 5 106 149 250 1.44e-16 75.1
Msa1418070 AT5G61030.2 54.348 92 42 0 7 98 41 132 1.40e-22 90.9
Msa1418070 AT5G61030.1 54.348 92 42 0 7 98 41 132 1.40e-22 90.9
Msa1418070 AT5G06210.1 44.444 90 50 0 4 93 32 121 2.26e-21 84.3
Msa1418070 AT5G04280.1 45.161 93 51 0 7 99 8 100 2.50e-21 87.8
Msa1418070 AT1G74230.1 52.632 76 35 1 7 82 35 109 5.34e-21 86.3
Msa1418070 AT5G50250.1 50.000 80 40 0 6 85 207 286 5.99e-21 86.3
Msa1418070 AT5G50250.1 39.216 102 62 0 5 106 112 213 3.54e-16 73.6
Msa1418070 AT1G18630.1 50.000 78 39 0 7 84 37 114 1.11e-20 82.8
Msa1418070 AT5G47320.1 49.351 77 39 0 7 83 32 108 2.13e-19 80.9
Msa1418070 AT2G21690.1 47.126 87 41 1 4 85 5 91 2.23e-19 78.2
Msa1418070 AT1G60650.2 42.683 82 47 0 4 85 10 91 4.53e-19 81.3
Msa1418070 AT1G60650.1 42.683 82 47 0 4 85 10 91 4.53e-19 81.3
Msa1418070 AT2G37510.1 51.351 74 36 0 7 80 35 108 3.65e-18 75.9
Msa1418070 AT2G46780.1 43.269 104 52 3 7 104 23 125 4.22e-18 79.3
Msa1418070 AT2G46780.2 43.269 104 52 3 7 104 23 125 4.22e-18 79.3
Msa1418070 AT5G19960.1 35.200 125 79 2 8 132 9 131 5.94e-18 79.0
Msa1418070 AT1G22910.1 44.681 94 46 2 7 95 14 106 8.67e-18 77.4
Msa1418070 AT1G22910.2 44.681 94 46 2 7 95 14 106 1.04e-17 77.0
Msa1418070 AT1G22330.1 41.176 102 51 2 7 100 18 118 1.70e-17 77.0
Msa1418070 AT4G13860.1 45.205 73 40 0 7 79 4 76 3.49e-17 71.6
Msa1418070 AT1G22910.3 44.681 94 46 2 7 95 14 106 6.12e-17 76.3
Msa1418070 AT1G60000.1 45.122 82 45 0 2 83 173 254 6.49e-17 75.1
Msa1418070 AT3G08000.1 44.048 84 47 0 7 90 42 125 7.20e-17 72.4
Msa1418070 AT5G59865.1 45.679 81 44 0 7 87 27 107 8.17e-17 71.6
Msa1418070 AT1G20880.2 46.053 76 40 1 7 82 25 99 2.14e-16 73.9
Msa1418070 AT1G20880.3 46.053 76 40 1 7 82 25 99 2.14e-16 73.9
Msa1418070 AT1G20880.1 46.053 76 40 1 7 82 25 99 2.14e-16 73.9
Msa1418070 AT3G07810.1 40.506 79 45 1 7 85 7 83 2.94e-16 74.7
Msa1418070 AT3G07810.2 40.506 79 45 1 7 85 7 83 2.94e-16 74.7
Msa1418070 AT1G76460.1 46.053 76 40 1 7 82 25 99 8.10e-16 72.4
Msa1418070 AT1G33470.3 46.753 77 38 2 7 82 8 82 9.21e-16 71.6
Msa1418070 AT1G33470.1 46.753 77 38 2 7 82 8 82 1.05e-15 71.6
Msa1418070 AT1G33470.2 46.753 77 38 2 7 82 8 82 1.11e-15 71.6
Msa1418070 AT3G46020.1 38.272 81 50 0 4 84 5 85 1.63e-15 67.8
Msa1418070 AT2G33410.1 32.143 112 74 1 7 118 7 116 2.52e-15 72.0
Msa1418070 AT1G78260.1 43.421 76 42 1 7 82 18 92 5.64e-15 70.1
Msa1418070 AT4G26650.2 37.975 79 47 1 7 85 13 89 5.90e-15 70.9
Msa1418070 AT5G55550.5 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa1418070 AT5G55550.5 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa1418070 AT5G55550.4 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa1418070 AT5G55550.4 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa1418070 AT5G55550.9 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa1418070 AT5G55550.9 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa1418070 AT5G55550.7 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa1418070 AT5G55550.7 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa1418070 AT5G55550.1 39.241 79 46 1 7 85 7 83 6.17e-15 70.9
Msa1418070 AT5G55550.1 31.193 109 74 1 7 115 111 218 7.94e-12 62.0
Msa1418070 AT4G26650.1 37.975 79 47 1 7 85 16 92 6.52e-15 70.9
Msa1418070 AT5G55550.2 39.241 79 46 1 7 85 7 83 7.01e-15 70.9
Msa1418070 AT5G55550.2 31.193 109 74 1 7 115 111 218 7.05e-12 62.0
Msa1418070 AT5G55550.3 39.241 79 46 1 7 85 7 83 7.01e-15 70.9
Msa1418070 AT5G55550.3 31.193 109 74 1 7 115 111 218 7.05e-12 62.0
Msa1418070 AT5G55550.6 39.241 79 46 1 7 85 7 83 7.01e-15 70.9
Msa1418070 AT5G55550.6 31.193 109 74 1 7 115 111 218 7.05e-12 62.0
Msa1418070 AT5G55550.11 39.241 79 46 1 7 85 7 83 7.01e-15 70.9
Msa1418070 AT5G55550.11 31.193 109 74 1 7 115 111 218 7.05e-12 62.0
Msa1418070 AT1G78260.2 43.421 76 42 1 7 82 18 92 8.93e-15 69.3
Msa1418070 AT4G14300.1 37.975 79 47 1 7 85 7 83 1.06e-14 70.1
Msa1418070 AT4G14300.2 37.975 79 47 1 7 85 7 83 1.06e-14 70.1
Msa1418070 AT1G73530.1 36.250 80 51 0 7 86 78 157 3.49e-14 66.6
Msa1418070 AT5G54580.1 44.048 84 47 0 2 85 52 135 5.17e-14 65.5
Msa1418070 AT2G27330.1 29.348 92 65 0 8 99 23 114 5.44e-14 64.3
Msa1418070 AT1G01080.1 41.463 82 45 2 1 81 207 286 6.47e-14 67.4
Msa1418070 AT1G01080.2 41.463 82 45 2 1 81 208 287 7.00e-14 67.4
Msa1418070 AT2G23350.1 48.611 72 36 1 8 79 136 206 1.04e-13 67.4
Msa1418070 AT5G55550.8 40.845 71 40 1 7 77 7 75 1.04e-13 67.4
Msa1418070 AT5G55550.8 31.193 109 74 1 7 115 158 265 6.32e-12 62.4
Msa1418070 AT5G55550.10 40.845 71 40 1 7 77 7 75 1.15e-13 67.4
Msa1418070 AT5G55550.10 31.193 109 74 1 7 115 158 265 6.17e-12 62.4
Msa1418070 AT5G53720.1 43.590 78 43 1 5 82 8 84 1.19e-13 63.2
Msa1418070 AT3G20930.1 37.500 80 50 0 7 86 283 362 1.25e-13 67.0
Msa1418070 AT3G13224.2 34.568 81 53 0 7 87 110 190 1.40e-13 66.6
Msa1418070 AT3G13224.2 34.940 83 52 1 7 89 20 100 4.19e-13 65.5
Msa1418070 AT3G13224.3 34.409 93 55 2 1 89 1 91 2.03e-13 66.2
Msa1418070 AT3G13224.3 34.568 81 53 0 7 87 101 181 2.75e-13 65.9
Msa1418070 AT3G54770.1 42.105 76 43 1 7 82 18 92 3.13e-13 65.1
Msa1418070 AT3G54770.3 42.105 76 43 1 7 82 18 92 3.19e-13 65.1
Msa1418070 AT5G47620.1 35.443 79 49 1 7 85 7 83 4.00e-13 65.5
Msa1418070 AT5G47620.2 35.443 79 49 1 7 85 7 83 4.00e-13 65.5
Msa1418070 AT5G47620.5 35.443 79 49 1 7 85 7 83 4.00e-13 65.5
Msa1418070 AT4G13860.2 47.458 59 31 0 22 80 2 60 4.82e-13 60.8
Msa1418070 AT3G52150.2 44.156 77 43 0 6 82 177 253 7.23e-13 63.9
Msa1418070 AT3G52150.1 44.156 77 43 0 6 82 177 253 7.23e-13 63.9
Msa1418070 AT1G17640.2 38.750 80 47 1 7 86 67 144 8.20e-13 64.7
Msa1418070 AT1G17640.2 32.990 97 64 1 7 103 158 253 9.59e-13 64.3
Msa1418070 AT1G17640.1 38.750 80 47 1 7 86 67 144 8.20e-13 64.7
Msa1418070 AT1G17640.1 32.990 97 64 1 7 103 158 253 9.59e-13 64.3
Msa1418070 AT1G17640.3 38.750 80 47 1 7 86 67 144 8.20e-13 64.7
Msa1418070 AT1G17640.3 32.990 97 64 1 7 103 158 253 9.59e-13 64.3
Msa1418070 AT2G37510.2 38.384 99 36 1 7 80 35 133 1.48e-12 62.0
Msa1418070 AT2G18510.1 40.964 83 48 1 8 89 114 196 1.59e-12 63.9
Msa1418070 AT5G53680.1 49.254 67 27 2 7 66 14 80 2.38e-12 61.2
Msa1418070 AT3G14100.1 41.026 78 46 0 5 82 143 220 2.60e-12 63.2
Msa1418070 AT5G51300.1 42.308 78 45 0 5 82 479 556 4.68e-12 62.8
Msa1418070 AT5G51300.2 42.308 78 45 0 5 82 479 556 4.68e-12 62.8
Msa1418070 AT5G51300.3 42.308 78 45 0 5 82 479 556 4.68e-12 62.8
Msa1418070 AT1G54080.1 41.026 78 46 0 5 82 147 224 5.37e-12 62.4
Msa1418070 AT5G47620.4 36.111 72 44 1 7 78 7 76 5.52e-12 62.4
Msa1418070 AT5G40490.1 32.530 83 56 0 7 89 131 213 8.36e-12 62.0
Msa1418070 AT3G13224.1 34.524 84 53 1 7 90 20 101 1.07e-11 60.5
Msa1418070 AT5G11412.1 42.105 76 43 1 7 82 53 127 1.10e-11 59.7
Msa1418070 AT2G22100.1 38.272 81 49 1 8 88 165 244 1.48e-11 61.2
Msa1418070 AT3G20930.2 35.294 85 50 1 7 86 283 367 1.64e-11 60.8
Msa1418070 AT2G19380.1 45.614 57 31 0 9 65 411 467 2.01e-11 60.8
Msa1418070 AT2G22090.2 39.080 87 51 2 7 93 105 189 2.64e-11 60.5
Msa1418070 AT2G22090.1 39.080 87 51 2 7 93 105 189 2.80e-11 60.1
Msa1418070 AT5G40490.2 32.530 83 56 0 7 89 114 196 3.98e-11 60.1
Msa1418070 AT1G01080.3 42.857 56 32 0 1 56 207 262 4.69e-11 59.3

Find 100 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACTTCCGCCATGGAAAT+TGG 0.191164 tig0024534:+28674 None:intergenic
TAATGGCATGATGCCATTAA+TGG 0.236021 tig0024534:-27436 Msa1418070:three_prime_UTR
ATTCCAAATCTAAACCAATT+TGG 0.260003 tig0024534:+27754 None:intergenic
TAATGGCACGGTGCCATTAA+TGG 0.272692 tig0024534:+27539 None:intergenic
TAGCACTTTGGTGCCATTAA+TGG 0.277270 tig0024534:+27423 None:intergenic
AATTGGGGAAAGGGTTAAAA+TGG 0.277694 tig0024534:+28691 None:intergenic
AATCAATGGCTCAGATTTAA+TGG 0.279785 tig0024534:-27453 Msa1418070:three_prime_UTR
TATCTGTTATTATGCCAAAT+TGG 0.291052 tig0024534:-27768 Msa1418070:three_prime_UTR
ACTGGAAGATCAAGAGGATT+TGG 0.294131 tig0024534:-28250 Msa1418070:CDS
ATGCCAAATTGGTTTAGATT+TGG 0.298687 tig0024534:-27757 Msa1418070:three_prime_UTR
TAGCACTTTGGTGCCATTAA+TGG 0.302265 tig0024534:-27552 Msa1418070:three_prime_UTR
CGCGGAGGTGGTGGTGGATA+TGG 0.305180 tig0024534:-28088 Msa1418070:CDS
CGTCGTGGAGGTGGTGGATA+TGG 0.314693 tig0024534:-28052 Msa1418070:CDS
GAAATTGAATGGTCTGAATA+TGG 0.321676 tig0024534:-27793 Msa1418070:three_prime_UTR
TGGCACCGTGCCATTAAATC+TGG 0.334613 tig0024534:-27532 Msa1418070:three_prime_UTR
CAACTTCCGCCATGGAAATT+GGG 0.336480 tig0024534:+28675 None:intergenic
AAATAAACAGCATAAAGTTT+TGG 0.339669 tig0024534:+27629 None:intergenic
AGTGGCGGAGGCGGTGGTGG+TGG 0.340361 tig0024534:-28118 Msa1418070:CDS
GTTATCACCGATCGTGAAAC+TGG 0.362798 tig0024534:-28268 Msa1418070:CDS
TTTGTGTGGCCCAGATTTAA+TGG 0.364497 tig0024534:+27522 None:intergenic
ATCACCGCCTCTCGAGTATC+TGG 0.373006 tig0024534:+27883 None:intergenic
GGCGGAGGCGGTGGTGGTGG+TGG 0.379371 tig0024534:-28115 Msa1418070:CDS
GGCAGTGGCGGAGGCGGTGG+TGG 0.383736 tig0024534:-28121 Msa1418070:CDS
CGTGGCAGTGGCGGAGGCGG+TGG 0.400483 tig0024534:-28124 Msa1418070:CDS
GGATACCGCGGAGGTGGTGG+TGG 0.412147 tig0024534:-28094 Msa1418070:CDS
GGTGGTGGATATGGCGGAGG+TGG 0.417575 tig0024534:-28079 Msa1418070:CDS
GGTGGATACCGCGGAGGTGG+TGG 0.418043 tig0024534:-28097 Msa1418070:CDS
CGCCATGGAAATTGGGGAAA+GGG 0.422744 tig0024534:+28682 None:intergenic
GTGTTTGTGGTTCTGAGTAT+TGG 0.436138 tig0024534:-27730 Msa1418070:three_prime_UTR
TGTTCTTCATGTGGTTCTCC+TGG 0.437016 tig0024534:-27702 Msa1418070:three_prime_UTR
GGTGGTGAACGTCGTGGAGG+TGG 0.450443 tig0024534:-28061 Msa1418070:CDS
TAGATTTGGAATTGTGTTTG+TGG 0.450563 tig0024534:-27743 Msa1418070:three_prime_UTR
ATCTATCTTGGTGCAATCAA+TGG 0.452939 tig0024534:-27467 Msa1418070:three_prime_UTR
CAGAGTCGTGGCAGTGGCGG+AGG 0.453358 tig0024534:-28130 Msa1418070:CDS
GGTGGTGGATACCGCGGAGG+TGG 0.455995 tig0024534:-28100 Msa1418070:CDS
AGTCGTGGCAGTGGCGGAGG+CGG 0.456959 tig0024534:-28127 Msa1418070:CDS
GTGGCTCTGACAGTAGAAAC+TGG 0.459380 tig0024534:-27856 Msa1418070:CDS
GTTGAATACCGATGCTTTGT+TGG 0.464495 tig0024534:-28656 Msa1418070:CDS
CTTTGTTGGTGGACTTGCAT+GGG 0.465470 tig0024534:-28642 Msa1418070:CDS
GGATATGGTGGAGGTGGCTA+CGG 0.473053 tig0024534:-27917 Msa1418070:intron
GCCACACAACACATCTATCT+TGG 0.476186 tig0024534:-27479 Msa1418070:three_prime_UTR
AACCCTTTCCCCAATTTCCA+TGG 0.491236 tig0024534:-28684 Msa1418070:exon
CTCAAGAGCTTCGTTGTCAG+TGG 0.495714 tig0024534:+28619 None:intergenic
GGTGGTGGTGGATATGGCGG+AGG 0.496613 tig0024534:-28082 Msa1418070:CDS
GCGATGAAAGATGCGATTGA+AGG 0.503882 tig0024534:-28202 Msa1418070:CDS
ATGAACGGCCAAGATCTTGA+TGG 0.505893 tig0024534:-28178 Msa1418070:CDS
GTCAGTGAAGGGTAGCACTT+TGG 0.507921 tig0024534:-27564 Msa1418070:three_prime_UTR
GGTGGAGGTGGCTACGGAGA+TGG 0.509409 tig0024534:-27911 Msa1418070:intron
TGTCACATTCTCTACGGAGC+AGG 0.509697 tig0024534:-28224 Msa1418070:CDS
TTCACTGACGGGTAGCACTT+TGG 0.509972 tig0024534:+27411 None:intergenic
CGTGGAGGTGGTGGATATGG+TGG 0.512534 tig0024534:-28049 Msa1418070:CDS
TGAGTGTGAGTGAAATTGAA+TGG 0.516874 tig0024534:-27804 Msa1418070:three_prime_UTR
GGTGAACGTCGTGGAGGTGG+TGG 0.516929 tig0024534:-28058 Msa1418070:CDS
GCTTTGTTGGTGGACTTGCA+TGG 0.519425 tig0024534:-28643 Msa1418070:CDS
TGGATTTGTCACATTCTCTA+CGG 0.520808 tig0024534:-28230 Msa1418070:CDS
TGATCTTCCAGTTTCACGAT+CGG 0.521619 tig0024534:+28261 None:intergenic
GAGGCTCAGAGTCGTGGCAG+TGG 0.522443 tig0024534:-28136 Msa1418070:CDS
AACACATTGCCATTGCATCT+AGG 0.524549 tig0024534:-27501 Msa1418070:three_prime_UTR
TGTTACGTCCATCAAGATCT+TGG 0.527371 tig0024534:+28170 None:intergenic
TCGAGAGGCGGTGATGGCGG+TGG 0.528930 tig0024534:-27875 Msa1418070:CDS
AACTTCCGCCATGGAAATTG+GGG 0.531831 tig0024534:+28676 None:intergenic
ATTGGGGAAAGGGTTAAAAT+GGG 0.532461 tig0024534:+28692 None:intergenic
TATTCATACTTAGTCAGTGA+AGG 0.532979 tig0024534:-27576 Msa1418070:three_prime_UTR
GGAGGTGGTGGATATGGTGG+AGG 0.534655 tig0024534:-28046 Msa1418070:intron
CCGCCATGGAAATTGGGGAA+AGG 0.539529 tig0024534:+28681 None:intergenic
CGATGAAAGATGCGATTGAA+GGG 0.542649 tig0024534:-28201 Msa1418070:CDS
TGGTGAAATCGTCGAATCGA+AGG 0.542807 tig0024534:-28579 Msa1418070:intron
GCTCAGAGTCGTGGCAGTGG+CGG 0.545233 tig0024534:-28133 Msa1418070:CDS
CGATTCGACGATTTCACCAA+AGG 0.547099 tig0024534:+28583 None:intergenic
AGATACTCGAGAGGCGGTGA+TGG 0.555850 tig0024534:-27881 Msa1418070:CDS
GGAGGTGGCTACGGAGATGG+TGG 0.556120 tig0024534:-27908 Msa1418070:intron
GGCACCGTGCCATTAAATCT+GGG 0.560786 tig0024534:-27531 Msa1418070:three_prime_UTR
TTGGTTTCGTGTTCTTCATG+TGG 0.561892 tig0024534:-27711 Msa1418070:three_prime_UTR
CTTATAGTAGCAATAGAGTA+CGG 0.563266 tig0024534:+27601 None:intergenic
CAGTTCATCAAATGAATAGT+CGG 0.565020 tig0024534:-27662 Msa1418070:three_prime_UTR
CGTGAAACTGGAAGATCAAG+AGG 0.575579 tig0024534:-28256 Msa1418070:CDS
GAATACCGATGCTTTGTTGG+TGG 0.577401 tig0024534:-28653 Msa1418070:CDS
TACTCGAGAGGCGGTGATGG+CGG 0.579779 tig0024534:-27878 Msa1418070:CDS
ACGTAACATCACTGTGAATG+AGG 0.581570 tig0024534:-28155 Msa1418070:CDS
TCGGTATTCAACTTCCGCCA+TGG 0.595134 tig0024534:+28667 None:intergenic
ATTCATACTTAGTCAGTGAA+GGG 0.596444 tig0024534:-27575 Msa1418070:three_prime_UTR
GGAGGTGGTGGTGGATATGG+CGG 0.597374 tig0024534:-28085 Msa1418070:CDS
CTCTGACAGTAGAAACTGGA+GGG 0.619176 tig0024534:-27852 Msa1418070:CDS
GATGCGATTGAAGGGATGAA+CGG 0.619661 tig0024534:-28193 Msa1418070:CDS
GTGAATGAGGCTCAGAGTCG+TGG 0.619893 tig0024534:-28142 Msa1418070:CDS
GGTGGTGGTGGTGGATACCG+CGG 0.621729 tig0024534:-28106 Msa1418070:CDS
CAAGTCCACCAACAAAGCAT+CGG 0.636350 tig0024534:+28648 None:intergenic
GGCTCCAGATACTCGAGAGG+CGG 0.636811 tig0024534:-27887 Msa1418070:CDS
GGAGGTGGTGAACGTCGTGG+AGG 0.637604 tig0024534:-28064 Msa1418070:CDS
GCAATGGCAATGTGTTTGTG+TGG 0.639611 tig0024534:+27508 None:intergenic
GGCGGAGGTGGTGAACGTCG+TGG 0.655000 tig0024534:-28067 Msa1418070:CDS
GGTGGTGGTGGATACCGCGG+AGG 0.661432 tig0024534:-28103 Msa1418070:CDS
GTGGCCCAGATTTAATGGCA+CGG 0.676544 tig0024534:+27527 None:intergenic
GTTGTGTGGCCTAGATGCAA+TGG 0.682752 tig0024534:+27492 None:intergenic
CCTTTCCCCAATTTCCATGG+CGG 0.683414 tig0024534:-28681 Msa1418070:exon
CATATCCACCACCACCTCCG+CGG 0.713479 tig0024534:+28089 None:intergenic
GCTCTGACAGTAGAAACTGG+AGG 0.714944 tig0024534:-27853 Msa1418070:CDS
GTTCATATGAACAAACAACC+AGG 0.717060 tig0024534:+27684 None:intergenic
GGTGGCTCCAGATACTCGAG+AGG 0.728248 tig0024534:-27890 Msa1418070:CDS
ACCAAGATAGATGTGTTGTG+TGG 0.732234 tig0024534:+27478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTAATCACTTCAAAT+CGG - tig0024534:27759-27778 Msa1418070:three_prime_UTR 15.0%
!!! TTTTATTAATCACTTCAAAT+CGG - tig0024534:27759-27778 Msa1418070:three_prime_UTR 15.0%
!!! AAATAAACAGCATAAAGTTT+TGG + tig0024534:28541-28560 None:intergenic 20.0%
!!! AAATAAACAGCATAAAGTTT+TGG + tig0024534:28541-28560 None:intergenic 20.0%
!!! ACGATTCTTTGTTTTTGTTT+AGG - tig0024534:27877-27896 Msa1418070:CDS 25.0%
! TATCTGTTATTATGCCAAAT+TGG - tig0024534:28399-28418 Msa1418070:intron 25.0%
! ATTCCAAATCTAAACCAATT+TGG + tig0024534:28416-28435 None:intergenic 25.0%
!!! ACGATTCTTTGTTTTTGTTT+AGG - tig0024534:27877-27896 Msa1418070:CDS 25.0%
! TATCTGTTATTATGCCAAAT+TGG - tig0024534:28399-28418 Msa1418070:intron 25.0%
! ATTCCAAATCTAAACCAATT+TGG + tig0024534:28416-28435 None:intergenic 25.0%
ATGGGAAAACAAGAAAAAAG+AGG + tig0024534:27460-27479 None:intergenic 30.0%
TGTGTAAATTGAAGATCGAT+CGG - tig0024534:27800-27819 Msa1418070:three_prime_UTR 30.0%
GAAATTGAATGGTCTGAATA+TGG - tig0024534:28374-28393 Msa1418070:intron 30.0%
! ATGCCAAATTGGTTTAGATT+TGG - tig0024534:28410-28429 Msa1418070:intron 30.0%
! TAGATTTGGAATTGTGTTTG+TGG - tig0024534:28424-28443 Msa1418070:intron 30.0%
CAGTTCATCAAATGAATAGT+CGG - tig0024534:28505-28524 Msa1418070:intron 30.0%
CTTATAGTAGCAATAGAGTA+CGG + tig0024534:28569-28588 None:intergenic 30.0%
TATTCATACTTAGTCAGTGA+AGG - tig0024534:28591-28610 Msa1418070:CDS 30.0%
ATTCATACTTAGTCAGTGAA+GGG - tig0024534:28592-28611 Msa1418070:CDS 30.0%
AATCAATGGCTCAGATTTAA+TGG - tig0024534:28714-28733 Msa1418070:five_prime_UTR 30.0%
ATGGGAAAACAAGAAAAAAG+AGG + tig0024534:27460-27479 None:intergenic 30.0%
TGTGTAAATTGAAGATCGAT+CGG - tig0024534:27800-27819 Msa1418070:three_prime_UTR 30.0%
GAAATTGAATGGTCTGAATA+TGG - tig0024534:28374-28393 Msa1418070:intron 30.0%
! ATGCCAAATTGGTTTAGATT+TGG - tig0024534:28410-28429 Msa1418070:intron 30.0%
! TAGATTTGGAATTGTGTTTG+TGG - tig0024534:28424-28443 Msa1418070:intron 30.0%
CAGTTCATCAAATGAATAGT+CGG - tig0024534:28505-28524 Msa1418070:intron 30.0%
CTTATAGTAGCAATAGAGTA+CGG + tig0024534:28569-28588 None:intergenic 30.0%
TATTCATACTTAGTCAGTGA+AGG - tig0024534:28591-28610 Msa1418070:CDS 30.0%
ATTCATACTTAGTCAGTGAA+GGG - tig0024534:28592-28611 Msa1418070:CDS 30.0%
AATCAATGGCTCAGATTTAA+TGG - tig0024534:28714-28733 Msa1418070:five_prime_UTR 30.0%
ATTGGGGAAAGGGTTAAAAT+GGG + tig0024534:27478-27497 None:intergenic 35.0%
AATTGGGGAAAGGGTTAAAA+TGG + tig0024534:27479-27498 None:intergenic 35.0%
TGGATTTGTCACATTCTCTA+CGG - tig0024534:27937-27956 Msa1418070:intron 35.0%
TGAGTGTGAGTGAAATTGAA+TGG - tig0024534:28363-28382 Msa1418070:intron 35.0%
GTTCATATGAACAAACAACC+AGG + tig0024534:28486-28505 None:intergenic 35.0%
! ATCTATCTTGGTGCAATCAA+TGG - tig0024534:28700-28719 Msa1418070:five_prime_UTR 35.0%
TAATGGCATGATGCCATTAA+TGG - tig0024534:28731-28750 Msa1418070:five_prime_UTR 35.0%
ATTGGGGAAAGGGTTAAAAT+GGG + tig0024534:27478-27497 None:intergenic 35.0%
AATTGGGGAAAGGGTTAAAA+TGG + tig0024534:27479-27498 None:intergenic 35.0%
TGGATTTGTCACATTCTCTA+CGG - tig0024534:27937-27956 Msa1418070:intron 35.0%
TGAGTGTGAGTGAAATTGAA+TGG - tig0024534:28363-28382 Msa1418070:intron 35.0%
GTTCATATGAACAAACAACC+AGG + tig0024534:28486-28505 None:intergenic 35.0%
! ATCTATCTTGGTGCAATCAA+TGG - tig0024534:28700-28719 Msa1418070:five_prime_UTR 35.0%
TAATGGCATGATGCCATTAA+TGG - tig0024534:28731-28750 Msa1418070:five_prime_UTR 35.0%
GTTGAATACCGATGCTTTGT+TGG - tig0024534:27511-27530 Msa1418070:three_prime_UTR 40.0%
!!! GAGAAAGCGTTTTCTTCCTT+TGG - tig0024534:27568-27587 Msa1418070:three_prime_UTR 40.0%
TTTCCGATCTCAGCAACAAA+CGG + tig0024534:27615-27634 None:intergenic 40.0%
ATCGGAAATCTGACACGATT+CGG - tig0024534:27627-27646 Msa1418070:three_prime_UTR 40.0%
! AATCTGACACGATTCGGATT+TGG - tig0024534:27633-27652 Msa1418070:three_prime_UTR 40.0%
ACAGAACAGAACAGAACAGA+AGG + tig0024534:27672-27691 None:intergenic 40.0%
TGATCTTCCAGTTTCACGAT+CGG + tig0024534:27909-27928 None:intergenic 40.0%
ACTGGAAGATCAAGAGGATT+TGG - tig0024534:27917-27936 Msa1418070:intron 40.0%
CGATGAAAGATGCGATTGAA+GGG - tig0024534:27966-27985 Msa1418070:intron 40.0%
TGTTACGTCCATCAAGATCT+TGG + tig0024534:28000-28019 None:intergenic 40.0%
ACGTAACATCACTGTGAATG+AGG - tig0024534:28012-28031 Msa1418070:intron 40.0%
!! GTGTTTGTGGTTCTGAGTAT+TGG - tig0024534:28437-28456 Msa1418070:intron 40.0%
TTGGTTTCGTGTTCTTCATG+TGG - tig0024534:28456-28475 Msa1418070:intron 40.0%
!! TAGCACTTTGGTGCCATTAA+TGG - tig0024534:28615-28634 Msa1418070:CDS 40.0%
TTTGTGTGGCCCAGATTTAA+TGG + tig0024534:28648-28667 None:intergenic 40.0%
AACACATTGCCATTGCATCT+AGG - tig0024534:28666-28685 Msa1418070:CDS 40.0%
ACCAAGATAGATGTGTTGTG+TGG + tig0024534:28692-28711 None:intergenic 40.0%
GTTGAATACCGATGCTTTGT+TGG - tig0024534:27511-27530 Msa1418070:three_prime_UTR 40.0%
!!! GAGAAAGCGTTTTCTTCCTT+TGG - tig0024534:27568-27587 Msa1418070:three_prime_UTR 40.0%
TTTCCGATCTCAGCAACAAA+CGG + tig0024534:27615-27634 None:intergenic 40.0%
ATCGGAAATCTGACACGATT+CGG - tig0024534:27627-27646 Msa1418070:three_prime_UTR 40.0%
! AATCTGACACGATTCGGATT+TGG - tig0024534:27633-27652 Msa1418070:three_prime_UTR 40.0%
ACAGAACAGAACAGAACAGA+AGG + tig0024534:27672-27691 None:intergenic 40.0%
TGATCTTCCAGTTTCACGAT+CGG + tig0024534:27909-27928 None:intergenic 40.0%
ACTGGAAGATCAAGAGGATT+TGG - tig0024534:27917-27936 Msa1418070:intron 40.0%
CGATGAAAGATGCGATTGAA+GGG - tig0024534:27966-27985 Msa1418070:intron 40.0%
TGTTACGTCCATCAAGATCT+TGG + tig0024534:28000-28019 None:intergenic 40.0%
ACGTAACATCACTGTGAATG+AGG - tig0024534:28012-28031 Msa1418070:intron 40.0%
!! GTGTTTGTGGTTCTGAGTAT+TGG - tig0024534:28437-28456 Msa1418070:intron 40.0%
TTGGTTTCGTGTTCTTCATG+TGG - tig0024534:28456-28475 Msa1418070:intron 40.0%
!! TAGCACTTTGGTGCCATTAA+TGG - tig0024534:28615-28634 Msa1418070:CDS 40.0%
TTTGTGTGGCCCAGATTTAA+TGG + tig0024534:28648-28667 None:intergenic 40.0%
AACACATTGCCATTGCATCT+AGG - tig0024534:28666-28685 Msa1418070:CDS 40.0%
ACCAAGATAGATGTGTTGTG+TGG + tig0024534:28692-28711 None:intergenic 40.0%
AACCCTTTCCCCAATTTCCA+TGG - tig0024534:27483-27502 Msa1418070:three_prime_UTR 45.0%
AACTTCCGCCATGGAAATTG+GGG + tig0024534:27494-27513 None:intergenic 45.0%
CAACTTCCGCCATGGAAATT+GGG + tig0024534:27495-27514 None:intergenic 45.0%
TCAACTTCCGCCATGGAAAT+TGG + tig0024534:27496-27515 None:intergenic 45.0%
! GAATACCGATGCTTTGTTGG+TGG - tig0024534:27514-27533 Msa1418070:three_prime_UTR 45.0%
CAAGTCCACCAACAAAGCAT+CGG + tig0024534:27522-27541 None:intergenic 45.0%
!! CTTTGTTGGTGGACTTGCAT+GGG - tig0024534:27525-27544 Msa1418070:three_prime_UTR 45.0%
CGATTCGACGATTTCACCAA+AGG + tig0024534:27587-27606 None:intergenic 45.0%
TGGTGAAATCGTCGAATCGA+AGG - tig0024534:27588-27607 Msa1418070:three_prime_UTR 45.0%
GTTATCACCGATCGTGAAAC+TGG - tig0024534:27899-27918 Msa1418070:CDS 45.0%
CGTGAAACTGGAAGATCAAG+AGG - tig0024534:27911-27930 Msa1418070:intron 45.0%
GCGATGAAAGATGCGATTGA+AGG - tig0024534:27965-27984 Msa1418070:intron 45.0%
GATGCGATTGAAGGGATGAA+CGG - tig0024534:27974-27993 Msa1418070:intron 45.0%
ATGAACGGCCAAGATCTTGA+TGG - tig0024534:27989-28008 Msa1418070:intron 45.0%
CTCTGACAGTAGAAACTGGA+GGG - tig0024534:28315-28334 Msa1418070:intron 45.0%
TGTTCTTCATGTGGTTCTCC+TGG - tig0024534:28465-28484 Msa1418070:intron 45.0%
!! TAATGGCACGGTGCCATTAA+TGG + tig0024534:28631-28650 None:intergenic 45.0%
GCAATGGCAATGTGTTTGTG+TGG + tig0024534:28662-28681 None:intergenic 45.0%
GCCACACAACACATCTATCT+TGG - tig0024534:28688-28707 Msa1418070:five_prime_UTR 45.0%
AACCCTTTCCCCAATTTCCA+TGG - tig0024534:27483-27502 Msa1418070:three_prime_UTR 45.0%
AACTTCCGCCATGGAAATTG+GGG + tig0024534:27494-27513 None:intergenic 45.0%
CAACTTCCGCCATGGAAATT+GGG + tig0024534:27495-27514 None:intergenic 45.0%
TCAACTTCCGCCATGGAAAT+TGG + tig0024534:27496-27515 None:intergenic 45.0%
! GAATACCGATGCTTTGTTGG+TGG - tig0024534:27514-27533 Msa1418070:three_prime_UTR 45.0%
CAAGTCCACCAACAAAGCAT+CGG + tig0024534:27522-27541 None:intergenic 45.0%
!! CTTTGTTGGTGGACTTGCAT+GGG - tig0024534:27525-27544 Msa1418070:three_prime_UTR 45.0%
CGATTCGACGATTTCACCAA+AGG + tig0024534:27587-27606 None:intergenic 45.0%
TGGTGAAATCGTCGAATCGA+AGG - tig0024534:27588-27607 Msa1418070:three_prime_UTR 45.0%
GTTATCACCGATCGTGAAAC+TGG - tig0024534:27899-27918 Msa1418070:CDS 45.0%
CGTGAAACTGGAAGATCAAG+AGG - tig0024534:27911-27930 Msa1418070:intron 45.0%
GCGATGAAAGATGCGATTGA+AGG - tig0024534:27965-27984 Msa1418070:intron 45.0%
GATGCGATTGAAGGGATGAA+CGG - tig0024534:27974-27993 Msa1418070:intron 45.0%
ATGAACGGCCAAGATCTTGA+TGG - tig0024534:27989-28008 Msa1418070:intron 45.0%
CTCTGACAGTAGAAACTGGA+GGG - tig0024534:28315-28334 Msa1418070:intron 45.0%
TGTTCTTCATGTGGTTCTCC+TGG - tig0024534:28465-28484 Msa1418070:intron 45.0%
!! TAATGGCACGGTGCCATTAA+TGG + tig0024534:28631-28650 None:intergenic 45.0%
GCAATGGCAATGTGTTTGTG+TGG + tig0024534:28662-28681 None:intergenic 45.0%
GCCACACAACACATCTATCT+TGG - tig0024534:28688-28707 Msa1418070:five_prime_UTR 45.0%
CGCCATGGAAATTGGGGAAA+GGG + tig0024534:27488-27507 None:intergenic 50.0%
CCTTTCCCCAATTTCCATGG+CGG - tig0024534:27486-27505 Msa1418070:three_prime_UTR 50.0%
TCGGTATTCAACTTCCGCCA+TGG + tig0024534:27503-27522 None:intergenic 50.0%
!! GCTTTGTTGGTGGACTTGCA+TGG - tig0024534:27524-27543 Msa1418070:three_prime_UTR 50.0%
CTCAAGAGCTTCGTTGTCAG+TGG + tig0024534:27551-27570 None:intergenic 50.0%
! GGTCCGTTTGTTGCTGAGAT+CGG - tig0024534:27609-27628 Msa1418070:three_prime_UTR 50.0%
TGTCACATTCTCTACGGAGC+AGG - tig0024534:27943-27962 Msa1418070:intron 50.0%
!! GTGGCTCTGACAGTAGAAAC+TGG - tig0024534:28311-28330 Msa1418070:intron 50.0%
GCTCTGACAGTAGAAACTGG+AGG - tig0024534:28314-28333 Msa1418070:intron 50.0%
GTCAGTGAAGGGTAGCACTT+TGG - tig0024534:28603-28622 Msa1418070:CDS 50.0%
! TGGCACCGTGCCATTAAATC+TGG - tig0024534:28635-28654 Msa1418070:CDS 50.0%
! GGCACCGTGCCATTAAATCT+GGG - tig0024534:28636-28655 Msa1418070:CDS 50.0%
GTGGCCCAGATTTAATGGCA+CGG + tig0024534:28643-28662 None:intergenic 50.0%
GTTGTGTGGCCTAGATGCAA+TGG + tig0024534:28678-28697 None:intergenic 50.0%
CGCCATGGAAATTGGGGAAA+GGG + tig0024534:27488-27507 None:intergenic 50.0%
CCTTTCCCCAATTTCCATGG+CGG - tig0024534:27486-27505 Msa1418070:three_prime_UTR 50.0%
TCGGTATTCAACTTCCGCCA+TGG + tig0024534:27503-27522 None:intergenic 50.0%
!! GCTTTGTTGGTGGACTTGCA+TGG - tig0024534:27524-27543 Msa1418070:three_prime_UTR 50.0%
CTCAAGAGCTTCGTTGTCAG+TGG + tig0024534:27551-27570 None:intergenic 50.0%
! GGTCCGTTTGTTGCTGAGAT+CGG - tig0024534:27609-27628 Msa1418070:three_prime_UTR 50.0%
TGTCACATTCTCTACGGAGC+AGG - tig0024534:27943-27962 Msa1418070:intron 50.0%
!! GTGGCTCTGACAGTAGAAAC+TGG - tig0024534:28311-28330 Msa1418070:intron 50.0%
GCTCTGACAGTAGAAACTGG+AGG - tig0024534:28314-28333 Msa1418070:intron 50.0%
GTCAGTGAAGGGTAGCACTT+TGG - tig0024534:28603-28622 Msa1418070:CDS 50.0%
! TGGCACCGTGCCATTAAATC+TGG - tig0024534:28635-28654 Msa1418070:CDS 50.0%
! GGCACCGTGCCATTAAATCT+GGG - tig0024534:28636-28655 Msa1418070:CDS 50.0%
GTGGCCCAGATTTAATGGCA+CGG + tig0024534:28643-28662 None:intergenic 50.0%
GTTGTGTGGCCTAGATGCAA+TGG + tig0024534:28678-28697 None:intergenic 50.0%
CCGCCATGGAAATTGGGGAA+AGG + tig0024534:27489-27508 None:intergenic 55.0%
GTGAATGAGGCTCAGAGTCG+TGG - tig0024534:28025-28044 Msa1418070:intron 55.0%
!! CGTGATGGTGGTGGTGGATA+TGG - tig0024534:28235-28254 Msa1418070:CDS 55.0%
!! GATGGTGGTGGTGGATATGG+TGG - tig0024534:28238-28257 Msa1418070:CDS 55.0%
! GGATATGGTGGAGGTGGCTA+CGG - tig0024534:28250-28269 Msa1418070:CDS 55.0%
ATCACCGCCTCTCGAGTATC+TGG + tig0024534:28287-28306 None:intergenic 55.0%
AGATACTCGAGAGGCGGTGA+TGG - tig0024534:28286-28305 Msa1418070:intron 55.0%
CCGCCATGGAAATTGGGGAA+AGG + tig0024534:27489-27508 None:intergenic 55.0%
GTGAATGAGGCTCAGAGTCG+TGG - tig0024534:28025-28044 Msa1418070:intron 55.0%
!! CGTGATGGTGGTGGTGGATA+TGG - tig0024534:28235-28254 Msa1418070:CDS 55.0%
!! GATGGTGGTGGTGGATATGG+TGG - tig0024534:28238-28257 Msa1418070:CDS 55.0%
! GGATATGGTGGAGGTGGCTA+CGG - tig0024534:28250-28269 Msa1418070:CDS 55.0%
ATCACCGCCTCTCGAGTATC+TGG + tig0024534:28287-28306 None:intergenic 55.0%
AGATACTCGAGAGGCGGTGA+TGG - tig0024534:28286-28305 Msa1418070:intron 55.0%
CATATCCACCACCACCTCCG+CGG + tig0024534:28081-28100 None:intergenic 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG - tig0024534:28082-28101 Msa1418070:CDS 60.0%
!! CGTCGTGGAGGTGGTGGATA+TGG - tig0024534:28115-28134 Msa1418070:CDS 60.0%
!! CGTGGAGGTGGTGGATATGG+TGG - tig0024534:28118-28137 Msa1418070:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG - tig0024534:28121-28140 Msa1418070:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG - tig0024534:28124-28143 Msa1418070:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+CGG - tig0024534:28127-28146 Msa1418070:CDS 60.0%
! GTGGATATGGTGGAGGTGGC+GGG - tig0024534:28128-28147 Msa1418070:CDS 60.0%
! TGGATATGGTGGAGGTGGCG+GGG - tig0024534:28129-28148 Msa1418070:CDS 60.0%
TATGGAGGTGGTGACCGTCG+TGG - tig0024534:28154-28173 Msa1418070:CDS 60.0%
ATCCACCACCTCCTCCACGA+CGG + tig0024534:28171-28190 None:intergenic 60.0%
!! CGTGGAGGAGGTGGTGGATA+TGG - tig0024534:28172-28191 Msa1418070:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG - tig0024534:28175-28194 Msa1418070:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG - tig0024534:28121-28140 Msa1418070:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG - tig0024534:28124-28143 Msa1418070:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+CGG - tig0024534:28184-28203 Msa1418070:CDS 60.0%
! GTGGATATGGTGGAGGAGGC+GGG - tig0024534:28185-28204 Msa1418070:CDS 60.0%
! TGGATATGGTGGAGGAGGCG+GGG - tig0024534:28186-28205 Msa1418070:CDS 60.0%
!! GGTGGTGGTGGATATGGTGG+AGG - tig0024534:28241-28260 Msa1418070:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG - tig0024534:28124-28143 Msa1418070:CDS 60.0%
GGTGGCTCCAGATACTCGAG+AGG - tig0024534:28277-28296 Msa1418070:intron 60.0%
GGCTCCAGATACTCGAGAGG+CGG - tig0024534:28280-28299 Msa1418070:intron 60.0%
! TACTCGAGAGGCGGTGATGG+CGG - tig0024534:28289-28308 Msa1418070:intron 60.0%
CATATCCACCACCACCTCCG+CGG + tig0024534:28081-28100 None:intergenic 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG - tig0024534:28082-28101 Msa1418070:CDS 60.0%
!! CGTCGTGGAGGTGGTGGATA+TGG - tig0024534:28115-28134 Msa1418070:CDS 60.0%
!! CGTGGAGGTGGTGGATATGG+TGG - tig0024534:28118-28137 Msa1418070:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG - tig0024534:28121-28140 Msa1418070:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG - tig0024534:28124-28143 Msa1418070:CDS 60.0%
! GGTGGATATGGTGGAGGTGG+CGG - tig0024534:28127-28146 Msa1418070:CDS 60.0%
! GTGGATATGGTGGAGGTGGC+GGG - tig0024534:28128-28147 Msa1418070:CDS 60.0%
! TGGATATGGTGGAGGTGGCG+GGG - tig0024534:28129-28148 Msa1418070:CDS 60.0%
TATGGAGGTGGTGACCGTCG+TGG - tig0024534:28154-28173 Msa1418070:CDS 60.0%
ATCCACCACCTCCTCCACGA+CGG + tig0024534:28171-28190 None:intergenic 60.0%
!! CGTGGAGGAGGTGGTGGATA+TGG - tig0024534:28172-28191 Msa1418070:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG - tig0024534:28175-28194 Msa1418070:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG - tig0024534:28121-28140 Msa1418070:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG - tig0024534:28124-28143 Msa1418070:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+CGG - tig0024534:28184-28203 Msa1418070:CDS 60.0%
! GTGGATATGGTGGAGGAGGC+GGG - tig0024534:28185-28204 Msa1418070:CDS 60.0%
! TGGATATGGTGGAGGAGGCG+GGG - tig0024534:28186-28205 Msa1418070:CDS 60.0%
!! GGTGGTGGTGGATATGGTGG+AGG - tig0024534:28241-28260 Msa1418070:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG - tig0024534:28124-28143 Msa1418070:CDS 60.0%
GGTGGCTCCAGATACTCGAG+AGG - tig0024534:28277-28296 Msa1418070:intron 60.0%
GGCTCCAGATACTCGAGAGG+CGG - tig0024534:28280-28299 Msa1418070:intron 60.0%
! TACTCGAGAGGCGGTGATGG+CGG - tig0024534:28289-28308 Msa1418070:intron 60.0%
GAGGCTCAGAGTCGTGGCAG+TGG - tig0024534:28031-28050 Msa1418070:intron 65.0%
GCTCAGAGTCGTGGCAGTGG+CGG - tig0024534:28034-28053 Msa1418070:intron 65.0%
!! GGTGGTGGTGGTGGATACCG+CGG - tig0024534:28061-28080 Msa1418070:CDS 65.0%
!! CGCGGAGGTGGTGGTGGATA+TGG - tig0024534:28079-28098 Msa1418070:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG - tig0024534:28085-28104 Msa1418070:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG - tig0024534:28088-28107 Msa1418070:CDS 65.0%
GGAGGTGGTGAACGTCGTGG+AGG - tig0024534:28103-28122 Msa1418070:CDS 65.0%
GGTGGTGAACGTCGTGGAGG+TGG - tig0024534:28106-28125 Msa1418070:CDS 65.0%
GGTGAACGTCGTGGAGGTGG+TGG - tig0024534:28109-28128 Msa1418070:CDS 65.0%
! GGATATGGTGGAGGTGGCGG+GGG - tig0024534:28130-28149 Msa1418070:CDS 65.0%
! GGATATGGTGGAGGAGGCGG+GGG - tig0024534:28187-28206 Msa1418070:CDS 65.0%
!! GGTTACGGAGGTGGTGGTGG+CGG - tig0024534:28208-28227 Msa1418070:CDS 65.0%
!! GGACGTCGTGATGGTGGTGG+TGG - tig0024534:28229-28248 Msa1418070:CDS 65.0%
GGTGGAGGTGGCTACGGAGA+TGG - tig0024534:28256-28275 Msa1418070:CDS 65.0%
GGAGGTGGCTACGGAGATGG+TGG - tig0024534:28259-28278 Msa1418070:CDS 65.0%
GAGGCTCAGAGTCGTGGCAG+TGG - tig0024534:28031-28050 Msa1418070:intron 65.0%
GCTCAGAGTCGTGGCAGTGG+CGG - tig0024534:28034-28053 Msa1418070:intron 65.0%
!! GGTGGTGGTGGTGGATACCG+CGG - tig0024534:28061-28080 Msa1418070:CDS 65.0%
!! CGCGGAGGTGGTGGTGGATA+TGG - tig0024534:28079-28098 Msa1418070:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG - tig0024534:28085-28104 Msa1418070:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG - tig0024534:28088-28107 Msa1418070:CDS 65.0%
GGAGGTGGTGAACGTCGTGG+AGG - tig0024534:28103-28122 Msa1418070:CDS 65.0%
GGTGGTGAACGTCGTGGAGG+TGG - tig0024534:28106-28125 Msa1418070:CDS 65.0%
GGTGAACGTCGTGGAGGTGG+TGG - tig0024534:28109-28128 Msa1418070:CDS 65.0%
! GGATATGGTGGAGGTGGCGG+GGG - tig0024534:28130-28149 Msa1418070:CDS 65.0%
! GGATATGGTGGAGGAGGCGG+GGG - tig0024534:28187-28206 Msa1418070:CDS 65.0%
!! GGTTACGGAGGTGGTGGTGG+CGG - tig0024534:28208-28227 Msa1418070:CDS 65.0%
!! GGACGTCGTGATGGTGGTGG+TGG - tig0024534:28229-28248 Msa1418070:CDS 65.0%
GGTGGAGGTGGCTACGGAGA+TGG - tig0024534:28256-28275 Msa1418070:CDS 65.0%
GGAGGTGGCTACGGAGATGG+TGG - tig0024534:28259-28278 Msa1418070:CDS 65.0%
CAGAGTCGTGGCAGTGGCGG+AGG - tig0024534:28037-28056 Msa1418070:intron 70.0%
! AGTCGTGGCAGTGGCGGAGG+CGG - tig0024534:28040-28059 Msa1418070:intron 70.0%
!! GGTGGTGGTGGATACCGCGG+AGG - tig0024534:28064-28083 Msa1418070:CDS 70.0%
!! GGTGGTGGATACCGCGGAGG+TGG - tig0024534:28067-28086 Msa1418070:CDS 70.0%
GGTGGATACCGCGGAGGTGG+TGG - tig0024534:28070-28089 Msa1418070:CDS 70.0%
! GGATACCGCGGAGGTGGTGG+TGG - tig0024534:28073-28092 Msa1418070:CDS 70.0%
GGCGGAGGTGGTGAACGTCG+TGG - tig0024534:28100-28119 Msa1418070:CDS 70.0%
!! GGTGGAGGTGGCGGGGGTTA+TGG - tig0024534:28136-28155 Msa1418070:CDS 70.0%
!! GGAGGTGGCGGGGGTTATGG+AGG - tig0024534:28139-28158 Msa1418070:CDS 70.0%
!! GGTGGCGGGGGTTATGGAGG+TGG - tig0024534:28142-28161 Msa1418070:CDS 70.0%
GGAGGTGGTGACCGTCGTGG+AGG - tig0024534:28157-28176 Msa1418070:CDS 70.0%
GGTGGTGACCGTCGTGGAGG+AGG - tig0024534:28160-28179 Msa1418070:CDS 70.0%
GGTGACCGTCGTGGAGGAGG+TGG - tig0024534:28163-28182 Msa1418070:CDS 70.0%
GACCGTCGTGGAGGAGGTGG+TGG - tig0024534:28166-28185 Msa1418070:CDS 70.0%
GGTGGAGGAGGCGGGGGTTA+CGG - tig0024534:28193-28212 Msa1418070:CDS 70.0%
! GGGGGTTACGGAGGTGGTGG+TGG - tig0024534:28205-28224 Msa1418070:CDS 70.0%
!! GGTGGTGGCGGACGTCGTGA+TGG - tig0024534:28220-28239 Msa1418070:CDS 70.0%
!! GGTGGCGGACGTCGTGATGG+TGG - tig0024534:28223-28242 Msa1418070:CDS 70.0%
!! GGCGGACGTCGTGATGGTGG+TGG - tig0024534:28226-28245 Msa1418070:CDS 70.0%
!! TCGAGAGGCGGTGATGGCGG+TGG - tig0024534:28292-28311 Msa1418070:intron 70.0%
CAGAGTCGTGGCAGTGGCGG+AGG - tig0024534:28037-28056 Msa1418070:intron 70.0%
! AGTCGTGGCAGTGGCGGAGG+CGG - tig0024534:28040-28059 Msa1418070:intron 70.0%
!! GGTGGTGGTGGATACCGCGG+AGG - tig0024534:28064-28083 Msa1418070:CDS 70.0%
!! GGTGGTGGATACCGCGGAGG+TGG - tig0024534:28067-28086 Msa1418070:CDS 70.0%
GGTGGATACCGCGGAGGTGG+TGG - tig0024534:28070-28089 Msa1418070:CDS 70.0%
! GGATACCGCGGAGGTGGTGG+TGG - tig0024534:28073-28092 Msa1418070:CDS 70.0%
GGCGGAGGTGGTGAACGTCG+TGG - tig0024534:28100-28119 Msa1418070:CDS 70.0%
!! GGTGGAGGTGGCGGGGGTTA+TGG - tig0024534:28136-28155 Msa1418070:CDS 70.0%
!! GGAGGTGGCGGGGGTTATGG+AGG - tig0024534:28139-28158 Msa1418070:CDS 70.0%
!! GGTGGCGGGGGTTATGGAGG+TGG - tig0024534:28142-28161 Msa1418070:CDS 70.0%
GGAGGTGGTGACCGTCGTGG+AGG - tig0024534:28157-28176 Msa1418070:CDS 70.0%
GGTGGTGACCGTCGTGGAGG+AGG - tig0024534:28160-28179 Msa1418070:CDS 70.0%
GGTGACCGTCGTGGAGGAGG+TGG - tig0024534:28163-28182 Msa1418070:CDS 70.0%
GACCGTCGTGGAGGAGGTGG+TGG - tig0024534:28166-28185 Msa1418070:CDS 70.0%
GGTGGAGGAGGCGGGGGTTA+CGG - tig0024534:28193-28212 Msa1418070:CDS 70.0%
! GGGGGTTACGGAGGTGGTGG+TGG - tig0024534:28205-28224 Msa1418070:CDS 70.0%
!! GGTGGTGGCGGACGTCGTGA+TGG - tig0024534:28220-28239 Msa1418070:CDS 70.0%
!! GGTGGCGGACGTCGTGATGG+TGG - tig0024534:28223-28242 Msa1418070:CDS 70.0%
!! GGCGGACGTCGTGATGGTGG+TGG - tig0024534:28226-28245 Msa1418070:CDS 70.0%
!! TCGAGAGGCGGTGATGGCGG+TGG - tig0024534:28292-28311 Msa1418070:intron 70.0%
! AGTGGCGGAGGCGGTGGTGG+TGG - tig0024534:28049-28068 Msa1418070:CDS 75.0%
GGAGGAGGCGGGGGTTACGG+AGG - tig0024534:28196-28215 Msa1418070:CDS 75.0%
GGAGGCGGGGGTTACGGAGG+TGG - tig0024534:28199-28218 Msa1418070:CDS 75.0%
GGCGGGGGTTACGGAGGTGG+TGG - tig0024534:28202-28221 Msa1418070:CDS 75.0%
! AGTGGCGGAGGCGGTGGTGG+TGG - tig0024534:28049-28068 Msa1418070:CDS 75.0%
GGAGGAGGCGGGGGTTACGG+AGG - tig0024534:28196-28215 Msa1418070:CDS 75.0%
GGAGGCGGGGGTTACGGAGG+TGG - tig0024534:28199-28218 Msa1418070:CDS 75.0%
GGCGGGGGTTACGGAGGTGG+TGG - tig0024534:28202-28221 Msa1418070:CDS 75.0%
!! CGTGGCAGTGGCGGAGGCGG+TGG - tig0024534:28043-28062 Msa1418070:intron 80.0%
!! GGCAGTGGCGGAGGCGGTGG+TGG - tig0024534:28046-28065 Msa1418070:intron 80.0%
!!! GGCGGAGGCGGTGGTGGTGG+TGG - tig0024534:28052-28071 Msa1418070:CDS 80.0%
!! CGTGGCAGTGGCGGAGGCGG+TGG - tig0024534:28043-28062 Msa1418070:intron 80.0%
!! GGCAGTGGCGGAGGCGGTGG+TGG - tig0024534:28046-28065 Msa1418070:intron 80.0%
!!! GGCGGAGGCGGTGGTGGTGG+TGG - tig0024534:28052-28071 Msa1418070:CDS 80.0%
Chromosome Type Strat End Strand Name
tig0024534 gene 27425 28764 27425 ID=Msa1418070;Name=Msa1418070
tig0024534 mRNA 27425 28764 27425 ID=Msa1418070-mRNA-1;Parent=Msa1418070;Name=Msa1418070-mRNA-1;_AED=0.12;_eAED=0.12;_QI=77|1|1|1|0.5|0.33|3|422|143
tig0024534 exon 27425 27926 27425 ID=Msa1418070-mRNA-1:exon:195;Parent=Msa1418070-mRNA-1
tig0024534 exon 28047 28290 28047 ID=Msa1418070-mRNA-1:exon:194;Parent=Msa1418070-mRNA-1
tig0024534 exon 28580 28764 28580 ID=Msa1418070-mRNA-1:exon:193;Parent=Msa1418070-mRNA-1
tig0024534 five_prime_UTR 28688 28764 28688 ID=Msa1418070-mRNA-1:five_prime_utr;Parent=Msa1418070-mRNA-1
tig0024534 CDS 28580 28687 28580 ID=Msa1418070-mRNA-1:cds;Parent=Msa1418070-mRNA-1
tig0024534 CDS 28047 28290 28047 ID=Msa1418070-mRNA-1:cds;Parent=Msa1418070-mRNA-1
tig0024534 CDS 27847 27926 27847 ID=Msa1418070-mRNA-1:cds;Parent=Msa1418070-mRNA-1
tig0024534 three_prime_UTR 27425 27846 27425 ID=Msa1418070-mRNA-1:three_prime_utr;Parent=Msa1418070-mRNA-1
Gene Sequence

>Msa1418070

ATGGCGGAAGTTGAATACCGATGCTTTGTTGGTGGACTTGCATGGGCCACTGACAACGAAGCTCTTGAGAAAGCGTTTTCTTCCTTTGGTGAAATCGTCGAATCGAAGGTTATCACCGATCGTGAAACTGGAAGATCAAGAGGATTTGGATTTGTCACATTCTCTACGGAGCAGGCGATGAAAGATGCGATTGAAGGGATGAACGGCCAAGATCTTGATGGACGTAACATCACTGTGAATGAGGCTCAGAGTCGTGGCAGTGGCGGAGGCGGTGGTGGTGGTGGATACCGCGGAGGTGGTGGTGGATATGGCGGAGGTGGTGAACGTCGTGGAGGTGGTGGATATGGTGGAGGTGGCTACGGAGATGGTGGCTCCAGATACTCGAGAGGCGGTGATGGCGGTGGCTCTGACAGTAGAAACTGGAGGGAATAA
Protein sequence

>Msa1418070

MAEVEYRCFVGGLAWATDNEALEKAFSSFGEIVESKVITDRETGRSRGFGFVTFSTEQAMKDAIEGMNGQDLDGRNITVNEAQSRGSGGGGGGGGYRGGGGGYGGGGERRGGGGYGGGGYGDGGSRYSRGGDGGGSDSRNWRE*