AlfalfaGEDB | Msa0523770

M. sativa cultivar zhongmu-4 / Msa0523770

KEGG: -
GO: -
PFAM: PF03087.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0523770	XP_003603947.1	80.000	70	14	0	1	70	54	123	1.58e-27	120
Msa0523770	XP_003603947.1	93.603	297	19	0	72	368	2	298	0.0	541

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0523770	G7J749	80.000	70	14	0	1	70	54	123	7.55e-28	120
Msa0523770	G7J749	93.603	297	19	0	72	368	2	298	0.0	541

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0356450	Msa0523770	0.810336	1.212693e-50	-8.615850e-47
Msa0523770	Msa1368490	0.855774	5.035574e-62	-8.615850e-47
Msa0523770	Msa1383300	0.826616	2.460395e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0523770	MtrunA17_Chr3g0144751	93.603	297	19	0	72	368	2	298	0.0	541
Msa0523770	MtrunA17_Chr3g0144751	80.000	70	14	0	1	70	54	123	1.45e-31	120
Msa0523770	MtrunA17_Chr3g0144781	83.946	299	42	3	72	368	2	296	1.05e-174	487
Msa0523770	MtrunA17_Chr3g0144781	88.889	72	8	0	1	72	56	127	1.23e-38	139
Msa0523770	MtrunA17_Chr4g0035261	58.609	302	114	7	74	368	4	301	4.33e-105	311
Msa0523770	MtrunA17_Chr4g0035261	70.588	68	20	0	4	71	61	128	7.01e-26	105
Msa0523770	MtrunA17_Chr4g0035251	57.333	300	118	6	75	368	8	303	3.03e-103	306
Msa0523770	MtrunA17_Chr4g0035251	69.118	68	21	0	4	71	59	126	8.95e-25	102
Msa0523770	MtrunA17_Chr4g0035231	45.763	295	152	6	77	368	7	296	8.11e-65	207
Msa0523770	MtrunA17_Chr4g0035231	50.704	71	35	0	1	71	55	125	5.18e-15	74.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0523770	AT2G17080.1	44.483	290	129	11	81	368	4	263	2.47e-57	187
Msa0523770	AT2G17080.1	49.296	71	36	0	1	71	48	118	3.23e-13	69.3
Msa0523770	AT2G17070.1	40.830	289	141	10	81	368	4	263	7.93e-54	179
Msa0523770	AT2G17070.1	49.296	71	36	0	1	71	48	118	9.14e-13	68.2
Msa0523770	AT4G35200.1	38.596	285	140	11	81	365	4	253	8.49e-47	160
Msa0523770	AT4G35210.1	37.544	285	140	10	81	365	4	250	2.34e-45	156
Msa0523770	AT4G35690.1	31.944	288	178	6	85	368	11	284	8.68e-37	135
Msa0523770	AT4G35710.1	32.292	288	176	7	85	368	11	283	5.28e-33	124
Msa0523770	AT2G17680.1	30.592	304	171	7	83	367	9	291	9.80e-30	116
Msa0523770	AT3G51400.1	34.158	202	120	5	170	367	82	274	6.17e-22	94.7

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Find 77 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTTGGTAAATTTGAA+GGG	0.535899	3_4:+77224473	Msa0523770:CDS
AAAATGGGTGAATAATTTGT+TGG	0.458594	3_4:+77219942	Msa0523770:CDS
AAACAAAGACCATCAAACAG+TGG	0.714988	3_4:+77224526	Msa0523770:CDS
AAAGTCAATCTCCAAAACCT+TGG	0.577340	3_4:+77224460	Msa0523770:CDS
AAATGAGGTAAATAACCTCT+TGG	0.511160	3_4:+77224262	Msa0523770:CDS
AACTCAAAATCCAATTTCCA+CGG	0.542254	3_4:+77224032	Msa0523770:CDS
AAGAGTTTCTTGCTCCCTAG+TGG	0.621626	3_4:+77224682	Msa0523770:CDS
AAGGGCTTGAGTTTCTATTC+AGG	0.382811	3_4:+77224840	Msa0523770:CDS
ACAAACATCTAATAGCCTTA+AGG	0.458051	3_4:-77224291	None:intergenic
ACAAGGGCATCTTGAGTGAG+TGG	0.601239	3_4:-77219904	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCCATAAGCATCAAGAAAAA+TGG	+	chr3_4:77219926-77219945	Msa0523770:CDS	30.0%
CCATAAGCATCAAGAAAAAT+GGG	+	chr3_4:77219927-77219946	Msa0523770:CDS	30.0%
TTCAATCCATCATAAGAAGA+AGG	+	chr3_4:77220043-77220062	Msa0523770:CDS	30.0%
TCAATCCATCATAAGAAGAA+GGG	+	chr3_4:77220044-77220063	Msa0523770:CDS	30.0%
TAAACACGTATGAATCAATG+AGG	+	chr3_4:77220155-77220174	Msa0523770:intron	30.0%
ATTTATAAAAGATAGCCACG+AGG	+	chr3_4:77220515-77220534	Msa0523770:intron	30.0%
TTTATAAAAGATAGCCACGA+GGG	+	chr3_4:77220516-77220535	Msa0523770:intron	30.0%
TTTGGTCACCGAAAAAAAAA+AGG	-	chr3_4:77220724-77220743	None:intergenic	30.0%
TTCAATGTGATCATAAGTGT+CGG	+	chr3_4:77220852-77220871	Msa0523770:intron	30.0%
CTCTATATCACATAGTAACA+TGG	-	chr3_4:77221020-77221039	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	77219853	77224910	77219853	ID=Msa0523770;Name=Msa0523770
chr3_4	mRNA	77219853	77224910	77219853	ID=Msa0523770-mRNA-1;Parent=Msa0523770;Name=Msa0523770-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|0\|0\|1\|0\|0\|2\|0\|368
chr3_4	exon	77219853	77220069	77219853	ID=Msa0523770-mRNA-1:exon:22598;Parent=Msa0523770-mRNA-1
chr3_4	exon	77224021	77224910	77224021	ID=Msa0523770-mRNA-1:exon:22599;Parent=Msa0523770-mRNA-1
chr3_4	CDS	77219853	77220069	77219853	ID=Msa0523770-mRNA-1:cds;Parent=Msa0523770-mRNA-1
chr3_4	CDS	77224021	77224910	77224021	ID=Msa0523770-mRNA-1:cds;Parent=Msa0523770-mRNA-1

Gene Sequence

>Msa0523770

ATTGGAGGCTTGCGAGATTTGGTCGAGTGCGTTCAAAATTTAATTCAGCTCCCACTCACTCAAGATGCCCTTGTCCATAAGCATCAAGAAAAATGGGTGAATAATTTGTTGGATGGATCCTTAAGACTCTTAGATGTGTGTAGTGCAACTAAAGATGCTTTATTTCACACAAAAGAATGCACAAGGGAACTTCAATCCATCATAAGAAGAAGGGGAGCCTCTCCTTTGAACTCAAAATCCAATTTCCACGGTCGCTCAATCAGCTTGCCATCTAGACCACACCCACTCATTCTTGAATGTAATGAGCATTTAGACACCTTGTTAAGACCTTCCAGTGAAACCTCCTCATCATCTTTGATTTGCCATAAGATTGATGGCTTGCGAGACTTGATCGAGTGTGTTGAAAAATTGGTTCAGCTTCCACTAACACAAGATGCACTTCTACATGAGCATCAAGAAAATGAGGTAAATAACCTCTTGGATGGATCCTTAAGGCTATTAGATGTTTGTAGTGCAGCTAAAGATGCTTTAATTCACACAAGAGAATGCACAAGGGAACTTCAATCCCTCATAAGAAGAAGCAAAAGAAGGGGATGCGGTGAAGTCGAACTCACTACAGAAGTTAAGAAGTTCTTGACATCCAGGAAGGTAGTGAGAAAGTCAATCTCCAAAACCTTGGTAAATTTGAAGGGAAATGCCAAAAATTGCAACATCTTATCTACAAACAAAGACCATCAAACAGTGGCTTTGATTAACTTATTACAAGATGTTGAAGTGGCTACCCTATCCACATTTCAGACAATATTGCAATTTATCTCCGGGACAACACAATCAAAGTCAAACAGCTGGGGTTCCATTTCCAAGCTAATTCAGCCCAAAAGAGTTTCTTGCTCCCTAGTGGCAGATGAAAGTGAATTTACCCAAGTGGATGGAGCATTGCAGTCTTTTGTACTTACCAAGACTAGAAAACTTGAAGCCATCAATGATCTGCAAAACCACTTAGAGAAAGCAGAGTCATGCATTCAAGACCTTGAAGAAGGGCTTGAGTTTCTATTCAGGCGTCTCATTAAAATTAGAGTTTCACTTCTTAATATTCTCAACCATTAA

Protein sequence

>Msa0523770

IGGLRDLVECVQNLIQLPLTQDALVHKHQEKWVNNLLDGSLRLLDVCSATKDALFHTKECTRELQSIIRRRGASPLNSKSNFHGRSISLPSRPHPLILECNEHLDTLLRPSSETSSSSLICHKIDGLRDLIECVEKLVQLPLTQDALLHEHQENEVNNLLDGSLRLLDVCSAAKDALIHTRECTRELQSLIRRSKRRGCGEVELTTEVKKFLTSRKVVRKSISKTLVNLKGNAKNCNILSTNKDHQTVALINLLQDVEVATLSTFQTILQFISGTTQSKSNSWGSISKLIQPKRVSCSLVADESEFTQVDGALQSFVLTKTRKLEAINDLQNHLEKAESCIQDLEEGLEFLFRRLIKIRVSLLNILNH*