AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0356450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0356450 MtrunA17_Chr3g0093001 98.913 92 1 0 1 92 1 92 1.82e-59 176
Msa0356450 MtrunA17_Chr7g0217141 71.277 94 24 2 1 92 1 93 4.75e-37 120
Msa0356450 MtrunA17_Chr5g0416431 71.429 77 20 1 17 91 17 93 8.94e-33 109
Msa0356450 MtrunA17_Chr6g0453041 71.795 78 20 1 17 92 15 92 7.44e-32 107
Msa0356450 MtrunA17_Chr8g0362131 65.476 84 26 2 10 91 10 92 1.18e-30 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0356450 AT3G47710.1 72.043 93 24 2 1 92 1 92 1.58e-38 124
Msa0356450 AT1G74500.1 76.623 77 17 1 17 92 17 93 5.15e-35 115
Msa0356450 AT1G26945.1 78.378 74 14 1 19 90 19 92 1.16e-33 112
Msa0356450 AT3G28857.1 64.894 94 28 3 1 91 1 92 1.02e-31 107
Msa0356450 AT5G39860.1 67.949 78 22 2 17 91 15 92 6.36e-29 99.8
Msa0356450 AT5G15160.1 65.333 75 23 2 19 90 18 92 1.23e-26 94.0

Find 18 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGCTTCAAGGGTGTTGC+GGG 0.358495 3_1:+35406188 Msa0356450:CDS
TGAGCGTTTGTCTGAGCTAT+TGG 0.392203 3_1:+35406260 Msa0356450:CDS
TATATCAGGTTTCAGCTTCA+AGG 0.403696 3_1:+35406178 Msa0356450:intron
ACTATATTAGAAACTTGAAC+AGG 0.414262 3_1:+35406220 Msa0356450:CDS
AGGAAGAAGTTGCTGCAACT+TGG 0.461097 3_1:-35405290 None:intergenic
GAGCTCCTATTGCGAATCTC+AGG 0.466230 3_1:-35405310 None:intergenic
TTCTTCCTGAGATTCGCAAT+AGG 0.484861 3_1:+35405305 Msa0356450:CDS
ATATCAGGTTTCAGCTTCAA+GGG 0.502020 3_1:+35406179 Msa0356450:intron
TTTCAGCTTCAAGGGTGTTG+CGG 0.507976 3_1:+35406187 Msa0356450:CDS
CTAGCAGGAGGTCGCGGTCA+CGG 0.548676 3_1:+35405215 Msa0356450:CDS
GCTCAGACAAACGCTCACTT+AGG 0.559789 3_1:-35406254 None:intergenic
GCAACTTGGAGACAAGATCA+TGG 0.561243 3_1:-35405276 None:intergenic
AGATGTCTAGCAGGAGGTCG+CGG 0.564343 3_1:+35405209 None:intergenic
CACGGCAAACAAGTAGTTCG+AGG 0.585931 3_1:+35405233 Msa0356450:CDS
TCGCAATAGGAGCTCTGACA+AGG 0.593557 3_1:+35405318 Msa0356450:CDS
AAGATCATGGATCTGATCGT+CGG 0.643584 3_1:-35405263 None:intergenic
TTATAAAGATGTCTAGCAGG+AGG 0.648667 3_1:+35405203 None:intergenic
CTATATTAGAAACTTGAACA+GGG 0.660572 3_1:+35406221 Msa0356450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTATAAAAGTCAT+TGG + chr3_1:35405471-35405490 Msa0356450:intron 10.0%
!! TGTATTAAAAATATAGTATA+TGG - chr3_1:35405565-35405584 None:intergenic 10.0%
!!! TTTTTTTTTTTTTCTTTTCT+TGG - chr3_1:35405884-35405903 None:intergenic 10.0%
!!! TTATTTTTTATAAAAGTCAT+TGG + chr3_1:35405471-35405490 Msa0356450:intron 10.0%
!! TGTATTAAAAATATAGTATA+TGG - chr3_1:35405565-35405584 None:intergenic 10.0%
!!! TTTTTTTTTTTTTCTTTTCT+TGG - chr3_1:35405884-35405903 None:intergenic 10.0%
!! GAAAAAAAAAAAAACTTGAA+AGG + chr3_1:35405890-35405909 Msa0356450:intron 15.0%
!!! AAATTGAATTTTACTATATC+AGG + chr3_1:35406164-35406183 Msa0356450:intron 15.0%
!! GAAAAAAAAAAAAACTTGAA+AGG + chr3_1:35405890-35405909 Msa0356450:intron 15.0%
!!! AAATTGAATTTTACTATATC+AGG + chr3_1:35406164-35406183 Msa0356450:intron 15.0%
! CTAGGAAACAAATAATATGT+AGG - chr3_1:35405350-35405369 None:intergenic 25.0%
!!! CTAGTCTTTTGATTAAAACT+AGG - chr3_1:35405368-35405387 None:intergenic 25.0%
!!! AGAAGAGATTAGTTTTATAG+CGG + chr3_1:35405586-35405605 Msa0356450:intron 25.0%
! GATAATGCATAATATATGCA+GGG + chr3_1:35405791-35405810 Msa0356450:intron 25.0%
! ATTTAAACCAACTTAATCCT+AGG + chr3_1:35405978-35405997 Msa0356450:intron 25.0%
! ACTATATTAGAAACTTGAAC+AGG + chr3_1:35406220-35406239 Msa0356450:CDS 25.0%
! CTATATTAGAAACTTGAACA+GGG + chr3_1:35406221-35406240 Msa0356450:CDS 25.0%
! CTAGGAAACAAATAATATGT+AGG - chr3_1:35405350-35405369 None:intergenic 25.0%
!!! CTAGTCTTTTGATTAAAACT+AGG - chr3_1:35405368-35405387 None:intergenic 25.0%
!!! AGAAGAGATTAGTTTTATAG+CGG + chr3_1:35405586-35405605 Msa0356450:intron 25.0%
! GATAATGCATAATATATGCA+GGG + chr3_1:35405791-35405810 Msa0356450:intron 25.0%
! ATTTAAACCAACTTAATCCT+AGG + chr3_1:35405978-35405997 Msa0356450:intron 25.0%
! ACTATATTAGAAACTTGAAC+AGG + chr3_1:35406220-35406239 Msa0356450:CDS 25.0%
! CTATATTAGAAACTTGAACA+GGG + chr3_1:35406221-35406240 Msa0356450:CDS 25.0%
GGATAATGCATAATATATGC+AGG + chr3_1:35405790-35405809 Msa0356450:intron 30.0%
ACCATACTTATTCACCTTTA+AGG + chr3_1:35405835-35405854 Msa0356450:intron 30.0%
ACCTTAAAGGTGAATAAGTA+TGG - chr3_1:35405839-35405858 None:intergenic 30.0%
TACACTAGAGTAAGTATAAG+AGG + chr3_1:35405953-35405972 Msa0356450:intron 30.0%
ACACTAGAGTAAGTATAAGA+GGG + chr3_1:35405954-35405973 Msa0356450:intron 30.0%
CAAGAAAAAGGAACAACAAT+AGG + chr3_1:35406014-35406033 Msa0356450:intron 30.0%
CATCAACAATTCTTGTAGAT+GGG - chr3_1:35406089-35406108 None:intergenic 30.0%
ACATCAACAATTCTTGTAGA+TGG - chr3_1:35406090-35406109 None:intergenic 30.0%
GGATAATGCATAATATATGC+AGG + chr3_1:35405790-35405809 Msa0356450:intron 30.0%
ACCATACTTATTCACCTTTA+AGG + chr3_1:35405835-35405854 Msa0356450:intron 30.0%
ACCTTAAAGGTGAATAAGTA+TGG - chr3_1:35405839-35405858 None:intergenic 30.0%
TACACTAGAGTAAGTATAAG+AGG + chr3_1:35405953-35405972 Msa0356450:intron 30.0%
ACACTAGAGTAAGTATAAGA+GGG + chr3_1:35405954-35405973 Msa0356450:intron 30.0%
CAAGAAAAAGGAACAACAAT+AGG + chr3_1:35406014-35406033 Msa0356450:intron 30.0%
CATCAACAATTCTTGTAGAT+GGG - chr3_1:35406089-35406108 None:intergenic 30.0%
ACATCAACAATTCTTGTAGA+TGG - chr3_1:35406090-35406109 None:intergenic 30.0%
GAGATACCCTATTAATCTAC+CGG + chr3_1:35405630-35405649 Msa0356450:intron 35.0%
TGAGCTTAGTTCATTTGGTA+AGG + chr3_1:35405768-35405787 Msa0356450:intron 35.0%
GAGCTTAGTTCATTTGGTAA+GGG + chr3_1:35405769-35405788 Msa0356450:intron 35.0%
!! TTATTCACCTTTAAGGTCTC+TGG + chr3_1:35405842-35405861 Msa0356450:intron 35.0%
! TTTCTTGGTCAATTAGCCTA+GGG - chr3_1:35405869-35405888 None:intergenic 35.0%
!! TTTTCTTGGTCAATTAGCCT+AGG - chr3_1:35405870-35405889 None:intergenic 35.0%
AATAGGCCTAACAAATACGA+GGG + chr3_1:35406031-35406050 Msa0356450:intron 35.0%
TTAATCCCCTCGTATTTGTT+AGG - chr3_1:35406040-35406059 None:intergenic 35.0%
TCTTGTAGATGGGAAAAAAC+TGG - chr3_1:35406079-35406098 None:intergenic 35.0%
TATATCAGGTTTCAGCTTCA+AGG + chr3_1:35406178-35406197 Msa0356450:intron 35.0%
ATATCAGGTTTCAGCTTCAA+GGG + chr3_1:35406179-35406198 Msa0356450:intron 35.0%
GAGATACCCTATTAATCTAC+CGG + chr3_1:35405630-35405649 Msa0356450:intron 35.0%
TGAGCTTAGTTCATTTGGTA+AGG + chr3_1:35405768-35405787 Msa0356450:intron 35.0%
GAGCTTAGTTCATTTGGTAA+GGG + chr3_1:35405769-35405788 Msa0356450:intron 35.0%
!! TTATTCACCTTTAAGGTCTC+TGG + chr3_1:35405842-35405861 Msa0356450:intron 35.0%
! TTTCTTGGTCAATTAGCCTA+GGG - chr3_1:35405869-35405888 None:intergenic 35.0%
!! TTTTCTTGGTCAATTAGCCT+AGG - chr3_1:35405870-35405889 None:intergenic 35.0%
AATAGGCCTAACAAATACGA+GGG + chr3_1:35406031-35406050 Msa0356450:intron 35.0%
TTAATCCCCTCGTATTTGTT+AGG - chr3_1:35406040-35406059 None:intergenic 35.0%
TCTTGTAGATGGGAAAAAAC+TGG - chr3_1:35406079-35406098 None:intergenic 35.0%
TATATCAGGTTTCAGCTTCA+AGG + chr3_1:35406178-35406197 Msa0356450:intron 35.0%
ATATCAGGTTTCAGCTTCAA+GGG + chr3_1:35406179-35406198 Msa0356450:intron 35.0%
AAGATCATGGATCTGATCGT+CGG - chr3_1:35405266-35405285 None:intergenic 40.0%
TTCTTCCTGAGATTCGCAAT+AGG + chr3_1:35405305-35405324 Msa0356450:CDS 40.0%
GACTCTCCGGTAGATTAATA+GGG - chr3_1:35405639-35405658 None:intergenic 40.0%
TGACTCTCCGGTAGATTAAT+AGG - chr3_1:35405640-35405659 None:intergenic 40.0%
!! GAGCTGTTTTGTGAGCTTTT+AGG - chr3_1:35405682-35405701 None:intergenic 40.0%
AGCTCAGAAGGACTTCTTTA+CGG - chr3_1:35405754-35405773 None:intergenic 40.0%
CCAAATGAACTAAGCTCAGA+AGG - chr3_1:35405766-35405785 None:intergenic 40.0%
CCTTCTGAGCTTAGTTCATT+TGG + chr3_1:35405763-35405782 Msa0356450:intron 40.0%
TGCATAATATATGCAGGGAC+CGG + chr3_1:35405796-35405815 Msa0356450:intron 40.0%
GTGAATAAGTATGGTGTCTG+GGG - chr3_1:35405830-35405849 None:intergenic 40.0%
GGTGAATAAGTATGGTGTCT+GGG - chr3_1:35405831-35405850 None:intergenic 40.0%
AGGTGAATAAGTATGGTGTC+TGG - chr3_1:35405832-35405851 None:intergenic 40.0%
! TTCTTGGTCAATTAGCCTAG+GGG - chr3_1:35405868-35405887 None:intergenic 40.0%
!! CCTTTTTCTTGTTATGTCCC+TGG - chr3_1:35406005-35406024 None:intergenic 40.0%
CCAGGGACATAACAAGAAAA+AGG + chr3_1:35406002-35406021 Msa0356450:intron 40.0%
CAATAGGCCTAACAAATACG+AGG + chr3_1:35406030-35406049 Msa0356450:intron 40.0%
ATAGGCCTAACAAATACGAG+GGG + chr3_1:35406032-35406051 Msa0356450:intron 40.0%
AAGATCATGGATCTGATCGT+CGG - chr3_1:35405266-35405285 None:intergenic 40.0%
TTCTTCCTGAGATTCGCAAT+AGG + chr3_1:35405305-35405324 Msa0356450:CDS 40.0%
GACTCTCCGGTAGATTAATA+GGG - chr3_1:35405639-35405658 None:intergenic 40.0%
TGACTCTCCGGTAGATTAAT+AGG - chr3_1:35405640-35405659 None:intergenic 40.0%
!! GAGCTGTTTTGTGAGCTTTT+AGG - chr3_1:35405682-35405701 None:intergenic 40.0%
AGCTCAGAAGGACTTCTTTA+CGG - chr3_1:35405754-35405773 None:intergenic 40.0%
CCAAATGAACTAAGCTCAGA+AGG - chr3_1:35405766-35405785 None:intergenic 40.0%
CCTTCTGAGCTTAGTTCATT+TGG + chr3_1:35405763-35405782 Msa0356450:intron 40.0%
TGCATAATATATGCAGGGAC+CGG + chr3_1:35405796-35405815 Msa0356450:intron 40.0%
GTGAATAAGTATGGTGTCTG+GGG - chr3_1:35405830-35405849 None:intergenic 40.0%
GGTGAATAAGTATGGTGTCT+GGG - chr3_1:35405831-35405850 None:intergenic 40.0%
AGGTGAATAAGTATGGTGTC+TGG - chr3_1:35405832-35405851 None:intergenic 40.0%
! TTCTTGGTCAATTAGCCTAG+GGG - chr3_1:35405868-35405887 None:intergenic 40.0%
!! CCTTTTTCTTGTTATGTCCC+TGG - chr3_1:35406005-35406024 None:intergenic 40.0%
CCAGGGACATAACAAGAAAA+AGG + chr3_1:35406002-35406021 Msa0356450:intron 40.0%
CAATAGGCCTAACAAATACG+AGG + chr3_1:35406030-35406049 Msa0356450:intron 40.0%
ATAGGCCTAACAAATACGAG+GGG + chr3_1:35406032-35406051 Msa0356450:intron 40.0%
GCAACTTGGAGACAAGATCA+TGG - chr3_1:35405279-35405298 None:intergenic 45.0%
AGGAAGAAGTTGCTGCAACT+TGG - chr3_1:35405293-35405312 None:intergenic 45.0%
ACCAACTTAATCCTAGGTCC+AGG + chr3_1:35405984-35406003 Msa0356450:intron 45.0%
CCAACTTAATCCTAGGTCCA+GGG + chr3_1:35405985-35406004 Msa0356450:intron 45.0%
!! TTTCAGCTTCAAGGGTGTTG+CGG + chr3_1:35406187-35406206 Msa0356450:CDS 45.0%
TGAGCGTTTGTCTGAGCTAT+TGG + chr3_1:35406260-35406279 Msa0356450:CDS 45.0%
GCAACTTGGAGACAAGATCA+TGG - chr3_1:35405279-35405298 None:intergenic 45.0%
AGGAAGAAGTTGCTGCAACT+TGG - chr3_1:35405293-35405312 None:intergenic 45.0%
ACCAACTTAATCCTAGGTCC+AGG + chr3_1:35405984-35406003 Msa0356450:intron 45.0%
CCAACTTAATCCTAGGTCCA+GGG + chr3_1:35405985-35406004 Msa0356450:intron 45.0%
!! TTTCAGCTTCAAGGGTGTTG+CGG + chr3_1:35406187-35406206 Msa0356450:CDS 45.0%
TGAGCGTTTGTCTGAGCTAT+TGG + chr3_1:35406260-35406279 Msa0356450:CDS 45.0%
CACGGCAAACAAGTAGTTCG+AGG + chr3_1:35405233-35405252 Msa0356450:CDS 50.0%
GAGCTCCTATTGCGAATCTC+AGG - chr3_1:35405313-35405332 None:intergenic 50.0%
! TCGCAATAGGAGCTCTGACA+AGG + chr3_1:35405318-35405337 Msa0356450:CDS 50.0%
! CATGGTTAGAGCTGACTCTC+CGG - chr3_1:35405652-35405671 None:intergenic 50.0%
! AGAGTCAGCTCTAACCATGG+TGG + chr3_1:35405653-35405672 Msa0356450:intron 50.0%
!! GAGCTTTTAGGAGTCCACCA+TGG - chr3_1:35405670-35405689 None:intergenic 50.0%
CCCTGGACCTAGGATTAAGT+TGG - chr3_1:35405988-35406007 None:intergenic 50.0%
! CTTGTTATGTCCCTGGACCT+AGG - chr3_1:35405998-35406017 None:intergenic 50.0%
!! TTCAGCTTCAAGGGTGTTGC+GGG + chr3_1:35406188-35406207 Msa0356450:CDS 50.0%
GCTCAGACAAACGCTCACTT+AGG - chr3_1:35406257-35406276 None:intergenic 50.0%
CACGGCAAACAAGTAGTTCG+AGG + chr3_1:35405233-35405252 Msa0356450:CDS 50.0%
GAGCTCCTATTGCGAATCTC+AGG - chr3_1:35405313-35405332 None:intergenic 50.0%
! TCGCAATAGGAGCTCTGACA+AGG + chr3_1:35405318-35405337 Msa0356450:CDS 50.0%
! CATGGTTAGAGCTGACTCTC+CGG - chr3_1:35405652-35405671 None:intergenic 50.0%
! AGAGTCAGCTCTAACCATGG+TGG + chr3_1:35405653-35405672 Msa0356450:intron 50.0%
!! GAGCTTTTAGGAGTCCACCA+TGG - chr3_1:35405670-35405689 None:intergenic 50.0%
CCCTGGACCTAGGATTAAGT+TGG - chr3_1:35405988-35406007 None:intergenic 50.0%
! CTTGTTATGTCCCTGGACCT+AGG - chr3_1:35405998-35406017 None:intergenic 50.0%
!! TTCAGCTTCAAGGGTGTTGC+GGG + chr3_1:35406188-35406207 Msa0356450:CDS 50.0%
GCTCAGACAAACGCTCACTT+AGG - chr3_1:35406257-35406276 None:intergenic 50.0%
! CGGAGAGTCAGCTCTAACCA+TGG + chr3_1:35405650-35405669 Msa0356450:intron 55.0%
CTAGGGGCCAGAGACCTTAA+AGG - chr3_1:35405852-35405871 None:intergenic 55.0%
!! CTTTAAGGTCTCTGGCCCCT+AGG + chr3_1:35405850-35405869 Msa0356450:intron 55.0%
! CGGAGAGTCAGCTCTAACCA+TGG + chr3_1:35405650-35405669 Msa0356450:intron 55.0%
CTAGGGGCCAGAGACCTTAA+AGG - chr3_1:35405852-35405871 None:intergenic 55.0%
!! CTTTAAGGTCTCTGGCCCCT+AGG + chr3_1:35405850-35405869 Msa0356450:intron 55.0%
! GGTGTCTGGGGTTCGAACTC+CGG - chr3_1:35405818-35405837 None:intergenic 60.0%
! GGTGTCTGGGGTTCGAACTC+CGG - chr3_1:35405818-35405837 None:intergenic 60.0%
CTAGCAGGAGGTCGCGGTCA+CGG + chr3_1:35405215-35405234 Msa0356450:CDS 65.0%
CTAGCAGGAGGTCGCGGTCA+CGG + chr3_1:35405215-35405234 Msa0356450:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 35405211 35406335 35405211 ID=Msa0356450;Name=Msa0356450
chr3_1 mRNA 35405211 35406335 35405211 ID=Msa0356450-mRNA-1;Parent=Msa0356450;Name=Msa0356450-mRNA-1;_AED=0.04;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|92
chr3_1 exon 35405211 35405339 35405211 ID=Msa0356450-mRNA-1:exon:6562;Parent=Msa0356450-mRNA-1
chr3_1 exon 35406186 35406335 35406186 ID=Msa0356450-mRNA-1:exon:6563;Parent=Msa0356450-mRNA-1
chr3_1 CDS 35405211 35405339 35405211 ID=Msa0356450-mRNA-1:cds;Parent=Msa0356450-mRNA-1
chr3_1 CDS 35406186 35406335 35406186 ID=Msa0356450-mRNA-1:cds;Parent=Msa0356450-mRNA-1
Gene Sequence

>Msa0356450

ATGTCTAGCAGGAGGTCGCGGTCACGGCAAACAAGTAGTTCGAGGAATATCACCGACGATCAGATCCATGATCTTGTCTCCAAGTTGCAGCAACTTCTTCCTGAGATTCGCAATAGGAGCTCTGACAAGGTTTCAGCTTCAAGGGTGTTGCGGGAGACTTGCAACTATATTAGAAACTTGAACAGGGAAGTTGACGACCTAAGTGAGCGTTTGTCTGAGCTATTGGCTACGACAGACACAGCACAAGCAGCAATAATTAGAAATTTACTTATGCAATAG

Protein sequence

>Msa0356450

MSSRRSRSRQTSSSRNITDDQIHDLVSKLQQLLPEIRNRSSDKVSASRVLRETCNYIRNLNREVDDLSERLSELLATTDTAQAAIIRNLLMQ*