AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0527810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0527810 MtrunA17_Chr4g0012821 98.750 80 1 0 1 80 1 80 2.17e-51 155
Msa0527810 MtrunA17_Chr8g0379051 85.366 82 10 1 1 80 1 82 4.67e-37 119
Msa0527810 MtrunA17_Chr1g0191471 59.494 79 24 1 10 80 26 104 1.67e-24 88.6
Msa0527810 MtrunA17_Chr2g0326321 60.241 83 27 1 4 80 21 103 9.17e-24 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0527810 AT3G60540.2 84.211 76 11 1 3 77 2 77 6.30e-35 114
Msa0527810 AT3G60540.3 84.211 76 11 1 3 77 2 77 6.30e-35 114
Msa0527810 AT3G60540.1 84.211 76 11 1 3 77 2 77 6.30e-35 114
Msa0527810 AT2G45070.1 84.810 79 8 1 3 77 2 80 5.10e-33 109
Msa0527810 AT2G45070.4 84.810 79 8 1 3 77 2 80 5.10e-33 109
Msa0527810 AT2G45070.2 84.810 79 8 1 3 77 2 80 5.10e-33 109
Msa0527810 AT2G45070.3 84.810 79 8 1 3 77 2 80 5.10e-33 109
Msa0527810 AT5G60460.2 58.333 84 27 1 5 80 22 105 1.76e-22 83.6
Msa0527810 AT5G60460.1 58.333 84 27 1 5 80 22 105 1.76e-22 83.6

Find 44 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGCAGCACTTCCTCTTT+GGG 0.197645 4_1:+464053 None:intergenic
TAGCTGCAGCACTTCCTCTT+TGG 0.251750 4_1:+464052 None:intergenic
CTTTGTCAGTACTTAGTAAT+AGG 0.295433 4_1:-463558 Msa0527810:three_prime_UTR
TGTACCTGAATGAGAAATTC+TGG 0.330483 4_1:+465101 None:intergenic
TCATTTCAGAAAATGGCTTT+AGG 0.333821 4_1:-464087 Msa0527810:intron
GTAACCAGAATTTCTCATTC+AGG 0.335677 4_1:-465105 Msa0527810:intron
GGAGCCTCTGGAGGAGCAGC+TGG 0.376820 4_1:-463994 Msa0527810:CDS
TTCTGGTTACTGAGATTTGT+AGG 0.387656 4_1:+465118 None:intergenic
ACACCAGGTGGTGGAGCCTC+TGG 0.397039 4_1:-464006 Msa0527810:CDS
TGTACTTGAGGGAGGCTTAA+GGG 0.407052 4_1:-463852 Msa0527810:exon
CCTCCAGAGGCTCCACCACC+TGG 0.407875 4_1:+464003 None:intergenic
GTTGTCATATATGTCATATA+TGG 0.424824 4_1:+463499 None:intergenic
CTGTACTTGAGGGAGGCTTA+AGG 0.429114 4_1:-463853 Msa0527810:exon
CAGAAAATGGCTTTAGGTGG+AGG 0.431685 4_1:-464081 Msa0527810:intron
GGGATGCAAGGCAAGAAGTT+GGG 0.467110 4_1:-463832 Msa0527810:three_prime_UTR
ATAAAGTATAACGACATATC+AGG 0.468466 4_1:+463477 None:intergenic
CGTGGGAAAGCTGTACTTGA+GGG 0.469860 4_1:-463863 Msa0527810:CDS
CTCATCACAAGAACAACATT+AGG 0.475458 4_1:+463922 None:intergenic
GTTGTTCTTGTGATGAGCAT+TGG 0.493707 4_1:-463916 Msa0527810:CDS
TGTTGCCGTCCTTCATGTCG+TGG 0.502055 4_1:-463881 Msa0527810:CDS
AGGGATGCAAGGCAAGAAGT+TGG 0.502672 4_1:-463833 Msa0527810:three_prime_UTR
GCTGCAGCACTTCCTCTTTG+GGG 0.514244 4_1:+464054 None:intergenic
AAAACAAATACAATATCAAA+GGG 0.529969 4_1:+463736 None:intergenic
ATTGTTCCTAGAAGTAAAAG+TGG 0.534496 4_1:-463645 Msa0527810:three_prime_UTR
CTACTGCAAGCATGAGGAGA+AGG 0.536447 4_1:-464032 Msa0527810:CDS
AGAAGGAAAACACCAGGTGG+TGG 0.545023 4_1:-464015 Msa0527810:CDS
GTTGCCGTCCTTCATGTCGT+GGG 0.549428 4_1:-463880 Msa0527810:CDS
TTTCAGAAAATGGCTTTAGG+TGG 0.554792 4_1:-464084 Msa0527810:intron
TTACTGAGATTTGTAGGAGT+TGG 0.567416 4_1:+465124 None:intergenic
GATTAATGCACACTACTACA+TGG 0.588875 4_1:-463446 Msa0527810:three_prime_UTR
CCAGGTGGTGGAGCCTCTGG+AGG 0.593869 4_1:-464003 Msa0527810:CDS
TTTAAGTCCAGGTGCATCGT+CGG 0.594963 4_1:+463951 None:intergenic
AGTTCCAGCTGCTCCTCCAG+AGG 0.601644 4_1:+463990 None:intergenic
TGTCGTTATACTTTATACAC+AGG 0.604631 4_1:-463470 Msa0527810:three_prime_UTR
AGGGAGGCTTAAGGGATGCA+AGG 0.606445 4_1:-463844 Msa0527810:exon
GGTGGAGGAGCTCCCCAAAG+AGG 0.606488 4_1:-464066 Msa0527810:CDS
ACAGCTTTCCCACGACATGA+AGG 0.608814 4_1:+463872 None:intergenic
ATGTCATATATGGTTAGCAA+CGG 0.621841 4_1:+463509 None:intergenic
TCGTGGGAAAGCTGTACTTG+AGG 0.624214 4_1:-463864 Msa0527810:CDS
CTGCAGCTACTGCAAGCATG+AGG 0.624920 4_1:-464038 Msa0527810:CDS
ATGAGGAGAAGGAAAACACC+AGG 0.632567 4_1:-464021 Msa0527810:CDS
CTTTCCCACGACATGAAGGA+CGG 0.643668 4_1:+463876 None:intergenic
GGGAAAGCTGTACTTGAGGG+AGG 0.648270 4_1:-463860 Msa0527810:CDS
AGGAGAAGGAAAACACCAGG+TGG 0.731216 4_1:-464018 Msa0527810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAATTATCTAATTTTTAAT+CGG - chr4_1:463643-463662 Msa0527810:three_prime_UTR 10.0%
!!! GTAATTATCTAATTTTTAAT+CGG - chr4_1:463643-463662 Msa0527810:three_prime_UTR 10.0%
!!! ATTTTCTGAATTTTGTTTAA+TGG - chr4_1:463547-463566 Msa0527810:three_prime_UTR 15.0%
!! AAAACAAATACAATATCAAA+GGG + chr4_1:464885-464904 None:intergenic 15.0%
!! AAAAACAAATACAATATCAA+AGG + chr4_1:464886-464905 None:intergenic 15.0%
!!! TATTACTTTTAAGTATAACA+AGG - chr4_1:464926-464945 Msa0527810:intron 15.0%
!!! ATTACTTTTAAGTATAACAA+GGG - chr4_1:464927-464946 Msa0527810:intron 15.0%
!!! ATTTTCTGAATTTTGTTTAA+TGG - chr4_1:463547-463566 Msa0527810:three_prime_UTR 15.0%
!! AAAACAAATACAATATCAAA+GGG + chr4_1:464885-464904 None:intergenic 15.0%
!! AAAAACAAATACAATATCAA+AGG + chr4_1:464886-464905 None:intergenic 15.0%
!!! TATTACTTTTAAGTATAACA+AGG - chr4_1:464926-464945 Msa0527810:intron 15.0%
!!! ATTACTTTTAAGTATAACAA+GGG - chr4_1:464927-464946 Msa0527810:intron 15.0%
!! TTCATCAAAATTCATAAACT+TGG + chr4_1:463671-463690 None:intergenic 20.0%
!!! TGTTTATTTGTTGAATGAAT+TGG - chr4_1:464040-464059 Msa0527810:CDS 20.0%
!!! ATTGAGAAACTTTTGTAAAT+TGG - chr4_1:464462-464481 Msa0527810:intron 20.0%
!!! ATTGGAAGTTAATTTGAATT+AGG - chr4_1:464480-464499 Msa0527810:intron 20.0%
!!! TTAATTTGAATTAGGTTTTG+TGG - chr4_1:464488-464507 Msa0527810:intron 20.0%
!!! TGTATTTGTTTTTGTTCTTT+TGG - chr4_1:464893-464912 Msa0527810:intron 20.0%
!! TTCATCAAAATTCATAAACT+TGG + chr4_1:463671-463690 None:intergenic 20.0%
!!! TGTTTATTTGTTGAATGAAT+TGG - chr4_1:464040-464059 Msa0527810:CDS 20.0%
!!! ATTGAGAAACTTTTGTAAAT+TGG - chr4_1:464462-464481 Msa0527810:intron 20.0%
!!! ATTGGAAGTTAATTTGAATT+AGG - chr4_1:464480-464499 Msa0527810:intron 20.0%
!!! TTAATTTGAATTAGGTTTTG+TGG - chr4_1:464488-464507 Msa0527810:intron 20.0%
!!! TGTATTTGTTTTTGTTCTTT+TGG - chr4_1:464893-464912 Msa0527810:intron 20.0%
! ATCTAAAAACCTTAATTGCA+TGG + chr4_1:463609-463628 None:intergenic 25.0%
! AAAACTGTCATAATACTTCT+CGG - chr4_1:463747-463766 Msa0527810:three_prime_UTR 25.0%
! AATGATAAGTATCTGACAAA+GGG + chr4_1:463784-463803 None:intergenic 25.0%
!! ACAATTCATATCAAGTGTTT+TGG + chr4_1:463940-463959 None:intergenic 25.0%
! TCTCATAACAATTTGAAAAC+AGG + chr4_1:464011-464030 None:intergenic 25.0%
!!! TGAATGAATTGGATTTTGAT+AGG - chr4_1:464051-464070 Msa0527810:CDS 25.0%
!! TCGAATATGTCTTTATTTGT+CGG - chr4_1:464211-464230 Msa0527810:intron 25.0%
! TGAACTTTCATTTCAGAAAA+TGG - chr4_1:464524-464543 Msa0527810:intron 25.0%
! AAAGTGAAAAACAGAAATCT+TGG - chr4_1:465086-465105 Msa0527810:intron 25.0%
! GTTGTCATATATGTCATATA+TGG + chr4_1:465122-465141 None:intergenic 25.0%
! ATAAAGTATAACGACATATC+AGG + chr4_1:465144-465163 None:intergenic 25.0%
! ATCTAAAAACCTTAATTGCA+TGG + chr4_1:463609-463628 None:intergenic 25.0%
! AAAACTGTCATAATACTTCT+CGG - chr4_1:463747-463766 Msa0527810:three_prime_UTR 25.0%
! AATGATAAGTATCTGACAAA+GGG + chr4_1:463784-463803 None:intergenic 25.0%
!! ACAATTCATATCAAGTGTTT+TGG + chr4_1:463940-463959 None:intergenic 25.0%
! TCTCATAACAATTTGAAAAC+AGG + chr4_1:464011-464030 None:intergenic 25.0%
!!! TGAATGAATTGGATTTTGAT+AGG - chr4_1:464051-464070 Msa0527810:CDS 25.0%
!! TCGAATATGTCTTTATTTGT+CGG - chr4_1:464211-464230 Msa0527810:intron 25.0%
! TGAACTTTCATTTCAGAAAA+TGG - chr4_1:464524-464543 Msa0527810:intron 25.0%
! AAAGTGAAAAACAGAAATCT+TGG - chr4_1:465086-465105 Msa0527810:intron 25.0%
! GTTGTCATATATGTCATATA+TGG + chr4_1:465122-465141 None:intergenic 25.0%
! ATAAAGTATAACGACATATC+AGG + chr4_1:465144-465163 None:intergenic 25.0%
TTTCGAATTCCATGCAATTA+AGG - chr4_1:463597-463616 Msa0527810:three_prime_UTR 30.0%
CAATGATAAGTATCTGACAA+AGG + chr4_1:463785-463804 None:intergenic 30.0%
! TCATTTTGAATGCAATTCTC+AGG - chr4_1:464101-464120 Msa0527810:intron 30.0%
CTTGATATTGTTGAGAATCA+TGG - chr4_1:464188-464207 Msa0527810:intron 30.0%
! ATATGTCTTTATTTGTCGGT+TGG - chr4_1:464215-464234 Msa0527810:intron 30.0%
TCATTTCAGAAAATGGCTTT+AGG - chr4_1:464531-464550 Msa0527810:intron 30.0%
!!! AAGAAGTTGGGTTCATTTTT+TGG - chr4_1:464798-464817 Msa0527810:intron 30.0%
ATTGTTCCTAGAAGTAAAAG+TGG - chr4_1:464973-464992 Msa0527810:intron 30.0%
!! CTTATACCACTTTTACTTCT+AGG + chr4_1:464982-465001 None:intergenic 30.0%
CTTTGTCAGTACTTAGTAAT+AGG - chr4_1:465060-465079 Msa0527810:intron 30.0%
ATGTCATATATGGTTAGCAA+CGG + chr4_1:465112-465131 None:intergenic 30.0%
TGTCGTTATACTTTATACAC+AGG - chr4_1:465148-465167 Msa0527810:five_prime_UTR 30.0%
TTTCGAATTCCATGCAATTA+AGG - chr4_1:463597-463616 Msa0527810:three_prime_UTR 30.0%
CAATGATAAGTATCTGACAA+AGG + chr4_1:463785-463804 None:intergenic 30.0%
! TCATTTTGAATGCAATTCTC+AGG - chr4_1:464101-464120 Msa0527810:intron 30.0%
CTTGATATTGTTGAGAATCA+TGG - chr4_1:464188-464207 Msa0527810:intron 30.0%
! ATATGTCTTTATTTGTCGGT+TGG - chr4_1:464215-464234 Msa0527810:intron 30.0%
TCATTTCAGAAAATGGCTTT+AGG - chr4_1:464531-464550 Msa0527810:intron 30.0%
!!! AAGAAGTTGGGTTCATTTTT+TGG - chr4_1:464798-464817 Msa0527810:intron 30.0%
ATTGTTCCTAGAAGTAAAAG+TGG - chr4_1:464973-464992 Msa0527810:intron 30.0%
!! CTTATACCACTTTTACTTCT+AGG + chr4_1:464982-465001 None:intergenic 30.0%
CTTTGTCAGTACTTAGTAAT+AGG - chr4_1:465060-465079 Msa0527810:intron 30.0%
ATGTCATATATGGTTAGCAA+CGG + chr4_1:465112-465131 None:intergenic 30.0%
TGTCGTTATACTTTATACAC+AGG - chr4_1:465148-465167 Msa0527810:five_prime_UTR 30.0%
TTACTGAGATTTGTAGGAGT+TGG + chr4_1:463497-463516 None:intergenic 35.0%
! TTCTGGTTACTGAGATTTGT+AGG + chr4_1:463503-463522 None:intergenic 35.0%
GTAACCAGAATTTCTCATTC+AGG - chr4_1:463513-463532 Msa0527810:three_prime_UTR 35.0%
TGTACCTGAATGAGAAATTC+TGG + chr4_1:463520-463539 None:intergenic 35.0%
GTCATAATACTTCTCGGATT+AGG - chr4_1:463753-463772 Msa0527810:three_prime_UTR 35.0%
! ATAGGTATTGCATTTGCATG+TGG - chr4_1:464069-464088 Msa0527810:CDS 35.0%
! TAGGTATTGCATTTGCATGT+GGG - chr4_1:464070-464089 Msa0527810:CDS 35.0%
GGAATACTAGAAAATGCTTG+TGG - chr4_1:464122-464141 Msa0527810:intron 35.0%
! TTGTCGGTTGGATAGAAATA+TGG - chr4_1:464227-464246 Msa0527810:intron 35.0%
ATGTCGTTGTTTGATGAATG+TGG - chr4_1:464266-464285 Msa0527810:intron 35.0%
!! TTTCAGAAAATGGCTTTAGG+TGG - chr4_1:464534-464553 Msa0527810:intron 35.0%
!! TTAGGTGAGATTTTAAGTCC+AGG + chr4_1:464681-464700 None:intergenic 35.0%
CTCATCACAAGAACAACATT+AGG + chr4_1:464699-464718 None:intergenic 35.0%
GATTAATGCACACTACTACA+TGG - chr4_1:465172-465191 Msa0527810:five_prime_UTR 35.0%
TTACTGAGATTTGTAGGAGT+TGG + chr4_1:463497-463516 None:intergenic 35.0%
! TTCTGGTTACTGAGATTTGT+AGG + chr4_1:463503-463522 None:intergenic 35.0%
GTAACCAGAATTTCTCATTC+AGG - chr4_1:463513-463532 Msa0527810:three_prime_UTR 35.0%
TGTACCTGAATGAGAAATTC+TGG + chr4_1:463520-463539 None:intergenic 35.0%
GTCATAATACTTCTCGGATT+AGG - chr4_1:463753-463772 Msa0527810:three_prime_UTR 35.0%
! ATAGGTATTGCATTTGCATG+TGG - chr4_1:464069-464088 Msa0527810:CDS 35.0%
! TAGGTATTGCATTTGCATGT+GGG - chr4_1:464070-464089 Msa0527810:CDS 35.0%
GGAATACTAGAAAATGCTTG+TGG - chr4_1:464122-464141 Msa0527810:intron 35.0%
! TTGTCGGTTGGATAGAAATA+TGG - chr4_1:464227-464246 Msa0527810:intron 35.0%
ATGTCGTTGTTTGATGAATG+TGG - chr4_1:464266-464285 Msa0527810:intron 35.0%
!! TTTCAGAAAATGGCTTTAGG+TGG - chr4_1:464534-464553 Msa0527810:intron 35.0%
!! TTAGGTGAGATTTTAAGTCC+AGG + chr4_1:464681-464700 None:intergenic 35.0%
CTCATCACAAGAACAACATT+AGG + chr4_1:464699-464718 None:intergenic 35.0%
GATTAATGCACACTACTACA+TGG - chr4_1:465172-465191 Msa0527810:five_prime_UTR 35.0%
GATATTGACATGTCTGTGTC+TGG - chr4_1:463894-463913 Msa0527810:CDS 40.0%
ACGTCTTATCCCACTAAGTA+AGG + chr4_1:464296-464315 None:intergenic 40.0%
TTACTTAGTGGGATAAGACG+TGG - chr4_1:464295-464314 Msa0527810:intron 40.0%
GTTGTTCTTGTGATGAGCAT+TGG - chr4_1:464702-464721 Msa0527810:intron 40.0%
GATATTGACATGTCTGTGTC+TGG - chr4_1:463894-463913 Msa0527810:CDS 40.0%
ACGTCTTATCCCACTAAGTA+AGG + chr4_1:464296-464315 None:intergenic 40.0%
TTACTTAGTGGGATAAGACG+TGG - chr4_1:464295-464314 Msa0527810:intron 40.0%
GTTGTTCTTGTGATGAGCAT+TGG - chr4_1:464702-464721 Msa0527810:intron 40.0%
ATGTGGCTGACCTTACTTAG+TGG - chr4_1:464283-464302 Msa0527810:intron 45.0%
TGTGGCTGACCTTACTTAGT+GGG - chr4_1:464284-464303 Msa0527810:intron 45.0%
!! CAGAAAATGGCTTTAGGTGG+AGG - chr4_1:464537-464556 Msa0527810:intron 45.0%
ATGAGGAGAAGGAAAACACC+AGG - chr4_1:464597-464616 Msa0527810:intron 45.0%
TTTAAGTCCAGGTGCATCGT+CGG + chr4_1:464670-464689 None:intergenic 45.0%
TGTACTTGAGGGAGGCTTAA+GGG - chr4_1:464766-464785 Msa0527810:intron 45.0%
ATGTGGCTGACCTTACTTAG+TGG - chr4_1:464283-464302 Msa0527810:intron 45.0%
TGTGGCTGACCTTACTTAGT+GGG - chr4_1:464284-464303 Msa0527810:intron 45.0%
!! CAGAAAATGGCTTTAGGTGG+AGG - chr4_1:464537-464556 Msa0527810:intron 45.0%
ATGAGGAGAAGGAAAACACC+AGG - chr4_1:464597-464616 Msa0527810:intron 45.0%
TTTAAGTCCAGGTGCATCGT+CGG + chr4_1:464670-464689 None:intergenic 45.0%
TGTACTTGAGGGAGGCTTAA+GGG - chr4_1:464766-464785 Msa0527810:intron 45.0%
ATCTTAAACCGCGACTGCGA+CGG + chr4_1:463726-463745 None:intergenic 50.0%
TCGTGTACTCGTCGTGTTCA+CGG + chr4_1:463820-463839 None:intergenic 50.0%
GACATGTCTGTGTCTGGTGT+CGG - chr4_1:463900-463919 Msa0527810:CDS 50.0%
AGCTGCAGCACTTCCTCTTT+GGG + chr4_1:464568-464587 None:intergenic 50.0%
TAGCTGCAGCACTTCCTCTT+TGG + chr4_1:464569-464588 None:intergenic 50.0%
CTACTGCAAGCATGAGGAGA+AGG - chr4_1:464586-464605 Msa0527810:intron 50.0%
AGGAGAAGGAAAACACCAGG+TGG - chr4_1:464600-464619 Msa0527810:intron 50.0%
AGAAGGAAAACACCAGGTGG+TGG - chr4_1:464603-464622 Msa0527810:intron 50.0%
! TTTTACACCGACGATGCACC+TGG - chr4_1:464660-464679 Msa0527810:intron 50.0%
CTTTCCCACGACATGAAGGA+CGG + chr4_1:464745-464764 None:intergenic 50.0%
ACAGCTTTCCCACGACATGA+AGG + chr4_1:464749-464768 None:intergenic 50.0%
TCGTGGGAAAGCTGTACTTG+AGG - chr4_1:464754-464773 Msa0527810:intron 50.0%
CGTGGGAAAGCTGTACTTGA+GGG - chr4_1:464755-464774 Msa0527810:intron 50.0%
CTGTACTTGAGGGAGGCTTA+AGG - chr4_1:464765-464784 Msa0527810:intron 50.0%
AGGGATGCAAGGCAAGAAGT+TGG - chr4_1:464785-464804 Msa0527810:intron 50.0%
GGGATGCAAGGCAAGAAGTT+GGG - chr4_1:464786-464805 Msa0527810:intron 50.0%
ATCTTAAACCGCGACTGCGA+CGG + chr4_1:463726-463745 None:intergenic 50.0%
TCGTGTACTCGTCGTGTTCA+CGG + chr4_1:463820-463839 None:intergenic 50.0%
GACATGTCTGTGTCTGGTGT+CGG - chr4_1:463900-463919 Msa0527810:CDS 50.0%
AGCTGCAGCACTTCCTCTTT+GGG + chr4_1:464568-464587 None:intergenic 50.0%
TAGCTGCAGCACTTCCTCTT+TGG + chr4_1:464569-464588 None:intergenic 50.0%
CTACTGCAAGCATGAGGAGA+AGG - chr4_1:464586-464605 Msa0527810:intron 50.0%
AGGAGAAGGAAAACACCAGG+TGG - chr4_1:464600-464619 Msa0527810:intron 50.0%
AGAAGGAAAACACCAGGTGG+TGG - chr4_1:464603-464622 Msa0527810:intron 50.0%
! TTTTACACCGACGATGCACC+TGG - chr4_1:464660-464679 Msa0527810:intron 50.0%
CTTTCCCACGACATGAAGGA+CGG + chr4_1:464745-464764 None:intergenic 50.0%
ACAGCTTTCCCACGACATGA+AGG + chr4_1:464749-464768 None:intergenic 50.0%
TCGTGGGAAAGCTGTACTTG+AGG - chr4_1:464754-464773 Msa0527810:intron 50.0%
CGTGGGAAAGCTGTACTTGA+GGG - chr4_1:464755-464774 Msa0527810:intron 50.0%
CTGTACTTGAGGGAGGCTTA+AGG - chr4_1:464765-464784 Msa0527810:intron 50.0%
AGGGATGCAAGGCAAGAAGT+TGG - chr4_1:464785-464804 Msa0527810:intron 50.0%
GGGATGCAAGGCAAGAAGTT+GGG - chr4_1:464786-464805 Msa0527810:intron 50.0%
TCTTAAACCGCGACTGCGAC+GGG + chr4_1:463725-463744 None:intergenic 55.0%
ACTCGTCGTGTTCACGGTGT+TGG + chr4_1:463814-463833 None:intergenic 55.0%
GCTGCAGCACTTCCTCTTTG+GGG + chr4_1:464567-464586 None:intergenic 55.0%
CTGCAGCTACTGCAAGCATG+AGG - chr4_1:464580-464599 Msa0527810:intron 55.0%
TGTTGCCGTCCTTCATGTCG+TGG - chr4_1:464737-464756 Msa0527810:intron 55.0%
GTTGCCGTCCTTCATGTCGT+GGG - chr4_1:464738-464757 Msa0527810:intron 55.0%
GGGAAAGCTGTACTTGAGGG+AGG - chr4_1:464758-464777 Msa0527810:intron 55.0%
AGGGAGGCTTAAGGGATGCA+AGG - chr4_1:464774-464793 Msa0527810:intron 55.0%
TCTTAAACCGCGACTGCGAC+GGG + chr4_1:463725-463744 None:intergenic 55.0%
ACTCGTCGTGTTCACGGTGT+TGG + chr4_1:463814-463833 None:intergenic 55.0%
GCTGCAGCACTTCCTCTTTG+GGG + chr4_1:464567-464586 None:intergenic 55.0%
CTGCAGCTACTGCAAGCATG+AGG - chr4_1:464580-464599 Msa0527810:intron 55.0%
TGTTGCCGTCCTTCATGTCG+TGG - chr4_1:464737-464756 Msa0527810:intron 55.0%
GTTGCCGTCCTTCATGTCGT+GGG - chr4_1:464738-464757 Msa0527810:intron 55.0%
GGGAAAGCTGTACTTGAGGG+AGG - chr4_1:464758-464777 Msa0527810:intron 55.0%
AGGGAGGCTTAAGGGATGCA+AGG - chr4_1:464774-464793 Msa0527810:intron 55.0%
CTTAAACCGCGACTGCGACG+GGG + chr4_1:463724-463743 None:intergenic 60.0%
AGTTCCAGCTGCTCCTCCAG+AGG + chr4_1:464631-464650 None:intergenic 60.0%
CTTAAACCGCGACTGCGACG+GGG + chr4_1:463724-463743 None:intergenic 60.0%
AGTTCCAGCTGCTCCTCCAG+AGG + chr4_1:464631-464650 None:intergenic 60.0%
GGTGGAGGAGCTCCCCAAAG+AGG - chr4_1:464552-464571 Msa0527810:intron 65.0%
!! ACACCAGGTGGTGGAGCCTC+TGG - chr4_1:464612-464631 Msa0527810:intron 65.0%
GGTGGAGGAGCTCCCCAAAG+AGG - chr4_1:464552-464571 Msa0527810:intron 65.0%
!! ACACCAGGTGGTGGAGCCTC+TGG - chr4_1:464612-464631 Msa0527810:intron 65.0%
CCTCCAGAGGCTCCACCACC+TGG + chr4_1:464618-464637 None:intergenic 70.0%
!! CCAGGTGGTGGAGCCTCTGG+AGG - chr4_1:464615-464634 Msa0527810:intron 70.0%
! GGAGCCTCTGGAGGAGCAGC+TGG - chr4_1:464624-464643 Msa0527810:intron 70.0%
CCTCCAGAGGCTCCACCACC+TGG + chr4_1:464618-464637 None:intergenic 70.0%
!! CCAGGTGGTGGAGCCTCTGG+AGG - chr4_1:464615-464634 Msa0527810:intron 70.0%
! GGAGCCTCTGGAGGAGCAGC+TGG - chr4_1:464624-464643 Msa0527810:intron 70.0%
GACGTGCCCCGTCGCAGTCG+CGG - chr4_1:463715-463734 Msa0527810:three_prime_UTR 75.0%
GACGTGCCCCGTCGCAGTCG+CGG - chr4_1:463715-463734 Msa0527810:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 463430 465210 463430 ID=Msa0527810;Name=Msa0527810
chr4_1 mRNA 463430 465210 463430 ID=Msa0527810-mRNA-1;Parent=Msa0527810;Name=Msa0527810-mRNA-1;_AED=0.00;_eAED=0.00;_QI=105|0|0.5|1|0|0.5|2|425|80
chr4_1 exon 463430 464097 463430 ID=Msa0527810-mRNA-1:exon:47;Parent=Msa0527810-mRNA-1
chr4_1 exon 465106 465210 465106 ID=Msa0527810-mRNA-1:exon:46;Parent=Msa0527810-mRNA-1
chr4_1 five_prime_UTR 465106 465210 465106 ID=Msa0527810-mRNA-1:five_prime_utr;Parent=Msa0527810-mRNA-1
chr4_1 CDS 463855 464097 463855 ID=Msa0527810-mRNA-1:cds;Parent=Msa0527810-mRNA-1
chr4_1 three_prime_UTR 463430 463854 463430 ID=Msa0527810-mRNA-1:three_prime_utr;Parent=Msa0527810-mRNA-1
Gene Sequence

>Msa0527810

ATGGCTTTAGGTGGAGGAGCTCCCCAAAGAGGAAGTGCTGCAGCTACTGCAAGCATGAGGAGAAGGAAAACACCAGGTGGTGGAGCCTCTGGAGGAGCAGCTGGAACTATGCTTCAATTTTACACCGACGATGCACCTGGACTTAAAATCTCACCTAATGTTGTTCTTGTGATGAGCATTGGCTTTATAGCATTTGTTGCCGTCCTTCATGTCGTGGGAAAGCTGTACTTGAGGGAGGCTTAA
Protein sequence

>Msa0527810

MALGGGAPQRGSAAATASMRRRKTPGGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMSIGFIAFVAVLHVVGKLYLREA*